Microencapsulation of growth factors by microfluidic system

The encapsulation of growth factors is an important component of tissue engineer- ing. Using microspheres is a convenient approach in which the dose of factors can be regulated by increasing or decreasing the number of encapsulated microspheres. Moreover, microspheres offer the possibility of delive...
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Autor*in:	Lilith M. Caballero-Aguilar [verfasserIn] Serena Duchi [verfasserIn] Anita Quigley [verfasserIn] Carmine Onofrillo [verfasserIn] Claudia Di Bella [verfasserIn] Simon E. Moulton [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	Microencapsulation Growth Factors Microfluidics Microspheres

Übergeordnetes Werk:	In: MethodsX - Elsevier, 2015, 8(2021), Seite 101324-
Übergeordnetes Werk:	volume:8 ; year:2021 ; pages:101324-

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.mex.2021.101324

Katalog-ID:	DOAJ074252410

Internformat


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520			\|a The encapsulation of growth factors is an important component of tissue engineer- ing. Using microspheres is a convenient approach in which the dose of factors can be regulated by increasing or decreasing the number of encapsulated microspheres. Moreover, microspheres offer the possibility of delivering the growth factors directly to the target site. However, the fabrication of microspheres by traditional emulsion methods is largely variable due to the experimental procedure. We have developed a protocol using a commercially available microfluidic system that allows formation of tunable particle-size droplets loaded with growth factors. The methodology includes a guide for preparing an alginate-growth factors solution followed by the specific set-up needed for using the microfluidic system to form the microspheres. The pro- cedure also includes a unique post-crosslinking process without pH modification. These methods allow the preservation of integrity and bioactivity of the growth factors tested (BMP-6 and TGFβ -3) and their subsequent sustained delivery. • The protocol can be tuned to form particles of various sizes. • The gentle post-crosslinking process allows conformational integrity of various bioactive molecules.
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publishDate	2021
allfields	10.1016/j.mex.2021.101324 doi (DE-627)DOAJ074252410 (DE-599)DOAJe0de673855344a5b986aafe8133fe29f DE-627 ger DE-627 rakwb eng Lilith M. Caballero-Aguilar verfasserin aut Microencapsulation of growth factors by microfluidic system 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The encapsulation of growth factors is an important component of tissue engineer- ing. Using microspheres is a convenient approach in which the dose of factors can be regulated by increasing or decreasing the number of encapsulated microspheres. Moreover, microspheres offer the possibility of delivering the growth factors directly to the target site. However, the fabrication of microspheres by traditional emulsion methods is largely variable due to the experimental procedure. We have developed a protocol using a commercially available microfluidic system that allows formation of tunable particle-size droplets loaded with growth factors. The methodology includes a guide for preparing an alginate-growth factors solution followed by the specific set-up needed for using the microfluidic system to form the microspheres. The pro- cedure also includes a unique post-crosslinking process without pH modification. These methods allow the preservation of integrity and bioactivity of the growth factors tested (BMP-6 and TGFβ -3) and their subsequent sustained delivery. • The protocol can be tuned to form particles of various sizes. • The gentle post-crosslinking process allows conformational integrity of various bioactive molecules. Microencapsulation Growth Factors Microfluidics Microspheres Science Q Serena Duchi verfasserin aut Anita Quigley verfasserin aut Carmine Onofrillo verfasserin aut Claudia Di Bella verfasserin aut Simon E. Moulton verfasserin aut In MethodsX Elsevier, 2015 8(2021), Seite 101324- (DE-627)832786675 (DE-600)2830212-6 22150161 nnns volume:8 year:2021 pages:101324- https://doi.org/10.1016/j.mex.2021.101324 kostenfrei https://doaj.org/article/e0de673855344a5b986aafe8133fe29f kostenfrei http://www.sciencedirect.com/science/article/pii/S2215016121001175 kostenfrei https://doaj.org/toc/2215-0161 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 8 2021 101324-
spelling	10.1016/j.mex.2021.101324 doi (DE-627)DOAJ074252410 (DE-599)DOAJe0de673855344a5b986aafe8133fe29f DE-627 ger DE-627 rakwb eng Lilith M. Caballero-Aguilar verfasserin aut Microencapsulation of growth factors by microfluidic system 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The encapsulation of growth factors is an important component of tissue engineer- ing. Using microspheres is a convenient approach in which the dose of factors can be regulated by increasing or decreasing the number of encapsulated microspheres. Moreover, microspheres offer the possibility of delivering the growth factors directly to the target site. However, the fabrication of microspheres by traditional emulsion methods is largely variable due to the experimental procedure. We have developed a protocol using a commercially available microfluidic system that allows formation of tunable particle-size droplets loaded with growth factors. The methodology includes a guide for preparing an alginate-growth factors solution followed by the specific set-up needed for using the microfluidic system to form the microspheres. The pro- cedure also includes a unique post-crosslinking process without pH modification. These methods allow the preservation of integrity and bioactivity of the growth factors tested (BMP-6 and TGFβ -3) and their subsequent sustained delivery. • The protocol can be tuned to form particles of various sizes. • The gentle post-crosslinking process allows conformational integrity of various bioactive molecules. Microencapsulation Growth Factors Microfluidics Microspheres Science Q Serena Duchi verfasserin aut Anita Quigley verfasserin aut Carmine Onofrillo verfasserin aut Claudia Di Bella verfasserin aut Simon E. Moulton verfasserin aut In MethodsX Elsevier, 2015 8(2021), Seite 101324- (DE-627)832786675 (DE-600)2830212-6 22150161 nnns volume:8 year:2021 pages:101324- https://doi.org/10.1016/j.mex.2021.101324 kostenfrei https://doaj.org/article/e0de673855344a5b986aafe8133fe29f kostenfrei http://www.sciencedirect.com/science/article/pii/S2215016121001175 kostenfrei https://doaj.org/toc/2215-0161 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 8 2021 101324-
allfields_unstemmed	10.1016/j.mex.2021.101324 doi (DE-627)DOAJ074252410 (DE-599)DOAJe0de673855344a5b986aafe8133fe29f DE-627 ger DE-627 rakwb eng Lilith M. Caballero-Aguilar verfasserin aut Microencapsulation of growth factors by microfluidic system 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The encapsulation of growth factors is an important component of tissue engineer- ing. Using microspheres is a convenient approach in which the dose of factors can be regulated by increasing or decreasing the number of encapsulated microspheres. Moreover, microspheres offer the possibility of delivering the growth factors directly to the target site. However, the fabrication of microspheres by traditional emulsion methods is largely variable due to the experimental procedure. We have developed a protocol using a commercially available microfluidic system that allows formation of tunable particle-size droplets loaded with growth factors. The methodology includes a guide for preparing an alginate-growth factors solution followed by the specific set-up needed for using the microfluidic system to form the microspheres. The pro- cedure also includes a unique post-crosslinking process without pH modification. These methods allow the preservation of integrity and bioactivity of the growth factors tested (BMP-6 and TGFβ -3) and their subsequent sustained delivery. • The protocol can be tuned to form particles of various sizes. • The gentle post-crosslinking process allows conformational integrity of various bioactive molecules. Microencapsulation Growth Factors Microfluidics Microspheres Science Q Serena Duchi verfasserin aut Anita Quigley verfasserin aut Carmine Onofrillo verfasserin aut Claudia Di Bella verfasserin aut Simon E. Moulton verfasserin aut In MethodsX Elsevier, 2015 8(2021), Seite 101324- (DE-627)832786675 (DE-600)2830212-6 22150161 nnns volume:8 year:2021 pages:101324- https://doi.org/10.1016/j.mex.2021.101324 kostenfrei https://doaj.org/article/e0de673855344a5b986aafe8133fe29f kostenfrei http://www.sciencedirect.com/science/article/pii/S2215016121001175 kostenfrei https://doaj.org/toc/2215-0161 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 8 2021 101324-
allfieldsGer	10.1016/j.mex.2021.101324 doi (DE-627)DOAJ074252410 (DE-599)DOAJe0de673855344a5b986aafe8133fe29f DE-627 ger DE-627 rakwb eng Lilith M. Caballero-Aguilar verfasserin aut Microencapsulation of growth factors by microfluidic system 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The encapsulation of growth factors is an important component of tissue engineer- ing. Using microspheres is a convenient approach in which the dose of factors can be regulated by increasing or decreasing the number of encapsulated microspheres. Moreover, microspheres offer the possibility of delivering the growth factors directly to the target site. However, the fabrication of microspheres by traditional emulsion methods is largely variable due to the experimental procedure. We have developed a protocol using a commercially available microfluidic system that allows formation of tunable particle-size droplets loaded with growth factors. The methodology includes a guide for preparing an alginate-growth factors solution followed by the specific set-up needed for using the microfluidic system to form the microspheres. The pro- cedure also includes a unique post-crosslinking process without pH modification. These methods allow the preservation of integrity and bioactivity of the growth factors tested (BMP-6 and TGFβ -3) and their subsequent sustained delivery. • The protocol can be tuned to form particles of various sizes. • The gentle post-crosslinking process allows conformational integrity of various bioactive molecules. Microencapsulation Growth Factors Microfluidics Microspheres Science Q Serena Duchi verfasserin aut Anita Quigley verfasserin aut Carmine Onofrillo verfasserin aut Claudia Di Bella verfasserin aut Simon E. Moulton verfasserin aut In MethodsX Elsevier, 2015 8(2021), Seite 101324- (DE-627)832786675 (DE-600)2830212-6 22150161 nnns volume:8 year:2021 pages:101324- https://doi.org/10.1016/j.mex.2021.101324 kostenfrei https://doaj.org/article/e0de673855344a5b986aafe8133fe29f kostenfrei http://www.sciencedirect.com/science/article/pii/S2215016121001175 kostenfrei https://doaj.org/toc/2215-0161 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 8 2021 101324-
allfieldsSound	10.1016/j.mex.2021.101324 doi (DE-627)DOAJ074252410 (DE-599)DOAJe0de673855344a5b986aafe8133fe29f DE-627 ger DE-627 rakwb eng Lilith M. Caballero-Aguilar verfasserin aut Microencapsulation of growth factors by microfluidic system 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The encapsulation of growth factors is an important component of tissue engineer- ing. Using microspheres is a convenient approach in which the dose of factors can be regulated by increasing or decreasing the number of encapsulated microspheres. Moreover, microspheres offer the possibility of delivering the growth factors directly to the target site. However, the fabrication of microspheres by traditional emulsion methods is largely variable due to the experimental procedure. We have developed a protocol using a commercially available microfluidic system that allows formation of tunable particle-size droplets loaded with growth factors. The methodology includes a guide for preparing an alginate-growth factors solution followed by the specific set-up needed for using the microfluidic system to form the microspheres. The pro- cedure also includes a unique post-crosslinking process without pH modification. These methods allow the preservation of integrity and bioactivity of the growth factors tested (BMP-6 and TGFβ -3) and their subsequent sustained delivery. • The protocol can be tuned to form particles of various sizes. • The gentle post-crosslinking process allows conformational integrity of various bioactive molecules. Microencapsulation Growth Factors Microfluidics Microspheres Science Q Serena Duchi verfasserin aut Anita Quigley verfasserin aut Carmine Onofrillo verfasserin aut Claudia Di Bella verfasserin aut Simon E. Moulton verfasserin aut In MethodsX Elsevier, 2015 8(2021), Seite 101324- (DE-627)832786675 (DE-600)2830212-6 22150161 nnns volume:8 year:2021 pages:101324- https://doi.org/10.1016/j.mex.2021.101324 kostenfrei https://doaj.org/article/e0de673855344a5b986aafe8133fe29f kostenfrei http://www.sciencedirect.com/science/article/pii/S2215016121001175 kostenfrei https://doaj.org/toc/2215-0161 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 8 2021 101324-
language	English
source	In MethodsX 8(2021), Seite 101324- volume:8 year:2021 pages:101324-
sourceStr	In MethodsX 8(2021), Seite 101324- volume:8 year:2021 pages:101324-
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Microencapsulation Growth Factors Microfluidics Microspheres Science Q
isfreeaccess_bool	true
container_title	MethodsX
authorswithroles_txt_mv	Lilith M. Caballero-Aguilar @@aut@@ Serena Duchi @@aut@@ Anita Quigley @@aut@@ Carmine Onofrillo @@aut@@ Claudia Di Bella @@aut@@ Simon E. Moulton @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	832786675
id	DOAJ074252410
language_de	englisch
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author	Lilith M. Caballero-Aguilar
spellingShingle	Lilith M. Caballero-Aguilar misc Microencapsulation misc Growth Factors misc Microfluidics misc Microspheres misc Science misc Q Microencapsulation of growth factors by microfluidic system
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topic_title	Microencapsulation of growth factors by microfluidic system Microencapsulation Growth Factors Microfluidics Microspheres
topic	misc Microencapsulation misc Growth Factors misc Microfluidics misc Microspheres misc Science misc Q
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title	Microencapsulation of growth factors by microfluidic system
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title_full	Microencapsulation of growth factors by microfluidic system
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title_sort	microencapsulation of growth factors by microfluidic system
title_auth	Microencapsulation of growth factors by microfluidic system
abstract	The encapsulation of growth factors is an important component of tissue engineer- ing. Using microspheres is a convenient approach in which the dose of factors can be regulated by increasing or decreasing the number of encapsulated microspheres. Moreover, microspheres offer the possibility of delivering the growth factors directly to the target site. However, the fabrication of microspheres by traditional emulsion methods is largely variable due to the experimental procedure. We have developed a protocol using a commercially available microfluidic system that allows formation of tunable particle-size droplets loaded with growth factors. The methodology includes a guide for preparing an alginate-growth factors solution followed by the specific set-up needed for using the microfluidic system to form the microspheres. The pro- cedure also includes a unique post-crosslinking process without pH modification. These methods allow the preservation of integrity and bioactivity of the growth factors tested (BMP-6 and TGFβ -3) and their subsequent sustained delivery. • The protocol can be tuned to form particles of various sizes. • The gentle post-crosslinking process allows conformational integrity of various bioactive molecules.
abstractGer	The encapsulation of growth factors is an important component of tissue engineer- ing. Using microspheres is a convenient approach in which the dose of factors can be regulated by increasing or decreasing the number of encapsulated microspheres. Moreover, microspheres offer the possibility of delivering the growth factors directly to the target site. However, the fabrication of microspheres by traditional emulsion methods is largely variable due to the experimental procedure. We have developed a protocol using a commercially available microfluidic system that allows formation of tunable particle-size droplets loaded with growth factors. The methodology includes a guide for preparing an alginate-growth factors solution followed by the specific set-up needed for using the microfluidic system to form the microspheres. The pro- cedure also includes a unique post-crosslinking process without pH modification. These methods allow the preservation of integrity and bioactivity of the growth factors tested (BMP-6 and TGFβ -3) and their subsequent sustained delivery. • The protocol can be tuned to form particles of various sizes. • The gentle post-crosslinking process allows conformational integrity of various bioactive molecules.
abstract_unstemmed	The encapsulation of growth factors is an important component of tissue engineer- ing. Using microspheres is a convenient approach in which the dose of factors can be regulated by increasing or decreasing the number of encapsulated microspheres. Moreover, microspheres offer the possibility of delivering the growth factors directly to the target site. However, the fabrication of microspheres by traditional emulsion methods is largely variable due to the experimental procedure. We have developed a protocol using a commercially available microfluidic system that allows formation of tunable particle-size droplets loaded with growth factors. The methodology includes a guide for preparing an alginate-growth factors solution followed by the specific set-up needed for using the microfluidic system to form the microspheres. The pro- cedure also includes a unique post-crosslinking process without pH modification. These methods allow the preservation of integrity and bioactivity of the growth factors tested (BMP-6 and TGFβ -3) and their subsequent sustained delivery. • The protocol can be tuned to form particles of various sizes. • The gentle post-crosslinking process allows conformational integrity of various bioactive molecules.
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title_short	Microencapsulation of growth factors by microfluidic system
url	https://doi.org/10.1016/j.mex.2021.101324 https://doaj.org/article/e0de673855344a5b986aafe8133fe29f http://www.sciencedirect.com/science/article/pii/S2215016121001175 https://doaj.org/toc/2215-0161
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Microencapsulation of growth factors by microfluidic system

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