Protective Effects of Eicosapentaenoic Acid Plus Hydroxytyrosol Supplementation Against White Adipose Tissue Abnormalities in Mice Fed a High-Fat Diet

Objective: Obesity induced by high-fat diet (HFD) elicits white adipose tissue dysfunction. In this study, we have hypothesized that the metabolic modulator eicosapentaenoic acid (EPA) combined with the antioxidant hydroxytyrosol (HT) attenuates HFD-induced white adipose tissue (WAT) alterations. Me...
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Autor*in:	Paola Illesca [verfasserIn] Rodrigo Valenzuela [verfasserIn] Alejandra Espinosa [verfasserIn] Francisca Echeverría [verfasserIn] Sandra Soto-Alarcon [verfasserIn] Cristian Campos [verfasserIn] Alicia Rodriguez [verfasserIn] Romina Vargas [verfasserIn] Thea Magrone [verfasserIn] Luis A. Videla [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	high-fat diet white adipose tissue adipocyte hypertrophy metabolic dysfunction eicosapentaenoic acid hydroxytyrosol

Übergeordnetes Werk:	In: Molecules - MDPI AG, 2003, 25(2020), 19, p 4433
Übergeordnetes Werk:	volume:25 ; year:2020 ; number:19, p 4433

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/molecules25194433

Katalog-ID:	DOAJ074337475

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author_variant	p i pi r v rv a e ae f e fe s s a ssa c c cc a r ar r v rv t m tm l a v lav
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allfields	10.3390/molecules25194433 doi (DE-627)DOAJ074337475 (DE-599)DOAJc12b117db0054d87a0bba256d250d9a8 DE-627 ger DE-627 rakwb eng QD241-441 Paola Illesca verfasserin aut Protective Effects of Eicosapentaenoic Acid Plus Hydroxytyrosol Supplementation Against White Adipose Tissue Abnormalities in Mice Fed a High-Fat Diet 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: Obesity induced by high-fat diet (HFD) elicits white adipose tissue dysfunction. In this study, we have hypothesized that the metabolic modulator eicosapentaenoic acid (EPA) combined with the antioxidant hydroxytyrosol (HT) attenuates HFD-induced white adipose tissue (WAT) alterations. Methods: C57BL/6J mice were administered with a HFD (60% fat, 20% protein, 20% carbohydrates) or control diet (CD; 10% fat, 20% protein, 70% carbohydrates), with or without EPA (50 mg/kg/day), HT (5 mg/kg/day), or both for 12 weeks. Determinations in WAT include morphological parameters, EPA and docosahexaenoic acid content in phospholipids (gas chromatography), lipogenesis, oxidative stress (OS) and inflammation markers, and gene expression and activities of transcription factors, such as sterol regulatory element-binding protein-1c (SREBP-1c), peroxisome proliferator-activated receptor-gamma (PPAR-γ), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) (p65 subunit) and nuclear factor erythroid 2-related factor 2 (Nrf2) (quantitative polymerase chain reaction and enzyme linked immunosorbent assay). Results: HFD led to WAT hypertrophy in relation to PPAR-γ downregulation. WAT metabolic dysfunction was characterized by upregulation of lipogenic SREBP-1c system, mitochondrial energy metabolism depression, loss of the antioxidant Nrf2 signaling with OS enhancement, n-3 long-chain polyunsaturated fatty acids depletion and activation of the pro-inflammatory NF-κB system. EPA and HT co-supplementation diminished HFD-dependent effects additively, reaching values close or similar to controls. Conclusion: Data presented strengthen the importance of combined protocols such as EPA plus HT to attenuate metabolic-inflammatory states triggered by obesity. high-fat diet white adipose tissue adipocyte hypertrophy metabolic dysfunction eicosapentaenoic acid hydroxytyrosol Organic chemistry Rodrigo Valenzuela verfasserin aut Alejandra Espinosa verfasserin aut Francisca Echeverría verfasserin aut Sandra Soto-Alarcon verfasserin aut Cristian Campos verfasserin aut Alicia Rodriguez verfasserin aut Romina Vargas verfasserin aut Thea Magrone verfasserin aut Luis A. Videla verfasserin aut In Molecules MDPI AG, 2003 25(2020), 19, p 4433 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:25 year:2020 number:19, p 4433 https://doi.org/10.3390/molecules25194433 kostenfrei https://doaj.org/article/c12b117db0054d87a0bba256d250d9a8 kostenfrei https://www.mdpi.com/1420-3049/25/19/4433 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2020 19, p 4433
spelling	10.3390/molecules25194433 doi (DE-627)DOAJ074337475 (DE-599)DOAJc12b117db0054d87a0bba256d250d9a8 DE-627 ger DE-627 rakwb eng QD241-441 Paola Illesca verfasserin aut Protective Effects of Eicosapentaenoic Acid Plus Hydroxytyrosol Supplementation Against White Adipose Tissue Abnormalities in Mice Fed a High-Fat Diet 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: Obesity induced by high-fat diet (HFD) elicits white adipose tissue dysfunction. In this study, we have hypothesized that the metabolic modulator eicosapentaenoic acid (EPA) combined with the antioxidant hydroxytyrosol (HT) attenuates HFD-induced white adipose tissue (WAT) alterations. Methods: C57BL/6J mice were administered with a HFD (60% fat, 20% protein, 20% carbohydrates) or control diet (CD; 10% fat, 20% protein, 70% carbohydrates), with or without EPA (50 mg/kg/day), HT (5 mg/kg/day), or both for 12 weeks. Determinations in WAT include morphological parameters, EPA and docosahexaenoic acid content in phospholipids (gas chromatography), lipogenesis, oxidative stress (OS) and inflammation markers, and gene expression and activities of transcription factors, such as sterol regulatory element-binding protein-1c (SREBP-1c), peroxisome proliferator-activated receptor-gamma (PPAR-γ), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) (p65 subunit) and nuclear factor erythroid 2-related factor 2 (Nrf2) (quantitative polymerase chain reaction and enzyme linked immunosorbent assay). Results: HFD led to WAT hypertrophy in relation to PPAR-γ downregulation. WAT metabolic dysfunction was characterized by upregulation of lipogenic SREBP-1c system, mitochondrial energy metabolism depression, loss of the antioxidant Nrf2 signaling with OS enhancement, n-3 long-chain polyunsaturated fatty acids depletion and activation of the pro-inflammatory NF-κB system. EPA and HT co-supplementation diminished HFD-dependent effects additively, reaching values close or similar to controls. Conclusion: Data presented strengthen the importance of combined protocols such as EPA plus HT to attenuate metabolic-inflammatory states triggered by obesity. high-fat diet white adipose tissue adipocyte hypertrophy metabolic dysfunction eicosapentaenoic acid hydroxytyrosol Organic chemistry Rodrigo Valenzuela verfasserin aut Alejandra Espinosa verfasserin aut Francisca Echeverría verfasserin aut Sandra Soto-Alarcon verfasserin aut Cristian Campos verfasserin aut Alicia Rodriguez verfasserin aut Romina Vargas verfasserin aut Thea Magrone verfasserin aut Luis A. Videla verfasserin aut In Molecules MDPI AG, 2003 25(2020), 19, p 4433 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:25 year:2020 number:19, p 4433 https://doi.org/10.3390/molecules25194433 kostenfrei https://doaj.org/article/c12b117db0054d87a0bba256d250d9a8 kostenfrei https://www.mdpi.com/1420-3049/25/19/4433 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2020 19, p 4433
allfields_unstemmed	10.3390/molecules25194433 doi (DE-627)DOAJ074337475 (DE-599)DOAJc12b117db0054d87a0bba256d250d9a8 DE-627 ger DE-627 rakwb eng QD241-441 Paola Illesca verfasserin aut Protective Effects of Eicosapentaenoic Acid Plus Hydroxytyrosol Supplementation Against White Adipose Tissue Abnormalities in Mice Fed a High-Fat Diet 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: Obesity induced by high-fat diet (HFD) elicits white adipose tissue dysfunction. In this study, we have hypothesized that the metabolic modulator eicosapentaenoic acid (EPA) combined with the antioxidant hydroxytyrosol (HT) attenuates HFD-induced white adipose tissue (WAT) alterations. Methods: C57BL/6J mice were administered with a HFD (60% fat, 20% protein, 20% carbohydrates) or control diet (CD; 10% fat, 20% protein, 70% carbohydrates), with or without EPA (50 mg/kg/day), HT (5 mg/kg/day), or both for 12 weeks. Determinations in WAT include morphological parameters, EPA and docosahexaenoic acid content in phospholipids (gas chromatography), lipogenesis, oxidative stress (OS) and inflammation markers, and gene expression and activities of transcription factors, such as sterol regulatory element-binding protein-1c (SREBP-1c), peroxisome proliferator-activated receptor-gamma (PPAR-γ), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) (p65 subunit) and nuclear factor erythroid 2-related factor 2 (Nrf2) (quantitative polymerase chain reaction and enzyme linked immunosorbent assay). Results: HFD led to WAT hypertrophy in relation to PPAR-γ downregulation. WAT metabolic dysfunction was characterized by upregulation of lipogenic SREBP-1c system, mitochondrial energy metabolism depression, loss of the antioxidant Nrf2 signaling with OS enhancement, n-3 long-chain polyunsaturated fatty acids depletion and activation of the pro-inflammatory NF-κB system. EPA and HT co-supplementation diminished HFD-dependent effects additively, reaching values close or similar to controls. Conclusion: Data presented strengthen the importance of combined protocols such as EPA plus HT to attenuate metabolic-inflammatory states triggered by obesity. high-fat diet white adipose tissue adipocyte hypertrophy metabolic dysfunction eicosapentaenoic acid hydroxytyrosol Organic chemistry Rodrigo Valenzuela verfasserin aut Alejandra Espinosa verfasserin aut Francisca Echeverría verfasserin aut Sandra Soto-Alarcon verfasserin aut Cristian Campos verfasserin aut Alicia Rodriguez verfasserin aut Romina Vargas verfasserin aut Thea Magrone verfasserin aut Luis A. Videla verfasserin aut In Molecules MDPI AG, 2003 25(2020), 19, p 4433 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:25 year:2020 number:19, p 4433 https://doi.org/10.3390/molecules25194433 kostenfrei https://doaj.org/article/c12b117db0054d87a0bba256d250d9a8 kostenfrei https://www.mdpi.com/1420-3049/25/19/4433 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2020 19, p 4433
allfieldsGer	10.3390/molecules25194433 doi (DE-627)DOAJ074337475 (DE-599)DOAJc12b117db0054d87a0bba256d250d9a8 DE-627 ger DE-627 rakwb eng QD241-441 Paola Illesca verfasserin aut Protective Effects of Eicosapentaenoic Acid Plus Hydroxytyrosol Supplementation Against White Adipose Tissue Abnormalities in Mice Fed a High-Fat Diet 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: Obesity induced by high-fat diet (HFD) elicits white adipose tissue dysfunction. In this study, we have hypothesized that the metabolic modulator eicosapentaenoic acid (EPA) combined with the antioxidant hydroxytyrosol (HT) attenuates HFD-induced white adipose tissue (WAT) alterations. Methods: C57BL/6J mice were administered with a HFD (60% fat, 20% protein, 20% carbohydrates) or control diet (CD; 10% fat, 20% protein, 70% carbohydrates), with or without EPA (50 mg/kg/day), HT (5 mg/kg/day), or both for 12 weeks. Determinations in WAT include morphological parameters, EPA and docosahexaenoic acid content in phospholipids (gas chromatography), lipogenesis, oxidative stress (OS) and inflammation markers, and gene expression and activities of transcription factors, such as sterol regulatory element-binding protein-1c (SREBP-1c), peroxisome proliferator-activated receptor-gamma (PPAR-γ), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) (p65 subunit) and nuclear factor erythroid 2-related factor 2 (Nrf2) (quantitative polymerase chain reaction and enzyme linked immunosorbent assay). Results: HFD led to WAT hypertrophy in relation to PPAR-γ downregulation. WAT metabolic dysfunction was characterized by upregulation of lipogenic SREBP-1c system, mitochondrial energy metabolism depression, loss of the antioxidant Nrf2 signaling with OS enhancement, n-3 long-chain polyunsaturated fatty acids depletion and activation of the pro-inflammatory NF-κB system. EPA and HT co-supplementation diminished HFD-dependent effects additively, reaching values close or similar to controls. Conclusion: Data presented strengthen the importance of combined protocols such as EPA plus HT to attenuate metabolic-inflammatory states triggered by obesity. high-fat diet white adipose tissue adipocyte hypertrophy metabolic dysfunction eicosapentaenoic acid hydroxytyrosol Organic chemistry Rodrigo Valenzuela verfasserin aut Alejandra Espinosa verfasserin aut Francisca Echeverría verfasserin aut Sandra Soto-Alarcon verfasserin aut Cristian Campos verfasserin aut Alicia Rodriguez verfasserin aut Romina Vargas verfasserin aut Thea Magrone verfasserin aut Luis A. Videla verfasserin aut In Molecules MDPI AG, 2003 25(2020), 19, p 4433 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:25 year:2020 number:19, p 4433 https://doi.org/10.3390/molecules25194433 kostenfrei https://doaj.org/article/c12b117db0054d87a0bba256d250d9a8 kostenfrei https://www.mdpi.com/1420-3049/25/19/4433 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2020 19, p 4433
allfieldsSound	10.3390/molecules25194433 doi (DE-627)DOAJ074337475 (DE-599)DOAJc12b117db0054d87a0bba256d250d9a8 DE-627 ger DE-627 rakwb eng QD241-441 Paola Illesca verfasserin aut Protective Effects of Eicosapentaenoic Acid Plus Hydroxytyrosol Supplementation Against White Adipose Tissue Abnormalities in Mice Fed a High-Fat Diet 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: Obesity induced by high-fat diet (HFD) elicits white adipose tissue dysfunction. In this study, we have hypothesized that the metabolic modulator eicosapentaenoic acid (EPA) combined with the antioxidant hydroxytyrosol (HT) attenuates HFD-induced white adipose tissue (WAT) alterations. Methods: C57BL/6J mice were administered with a HFD (60% fat, 20% protein, 20% carbohydrates) or control diet (CD; 10% fat, 20% protein, 70% carbohydrates), with or without EPA (50 mg/kg/day), HT (5 mg/kg/day), or both for 12 weeks. Determinations in WAT include morphological parameters, EPA and docosahexaenoic acid content in phospholipids (gas chromatography), lipogenesis, oxidative stress (OS) and inflammation markers, and gene expression and activities of transcription factors, such as sterol regulatory element-binding protein-1c (SREBP-1c), peroxisome proliferator-activated receptor-gamma (PPAR-γ), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) (p65 subunit) and nuclear factor erythroid 2-related factor 2 (Nrf2) (quantitative polymerase chain reaction and enzyme linked immunosorbent assay). Results: HFD led to WAT hypertrophy in relation to PPAR-γ downregulation. WAT metabolic dysfunction was characterized by upregulation of lipogenic SREBP-1c system, mitochondrial energy metabolism depression, loss of the antioxidant Nrf2 signaling with OS enhancement, n-3 long-chain polyunsaturated fatty acids depletion and activation of the pro-inflammatory NF-κB system. EPA and HT co-supplementation diminished HFD-dependent effects additively, reaching values close or similar to controls. Conclusion: Data presented strengthen the importance of combined protocols such as EPA plus HT to attenuate metabolic-inflammatory states triggered by obesity. high-fat diet white adipose tissue adipocyte hypertrophy metabolic dysfunction eicosapentaenoic acid hydroxytyrosol Organic chemistry Rodrigo Valenzuela verfasserin aut Alejandra Espinosa verfasserin aut Francisca Echeverría verfasserin aut Sandra Soto-Alarcon verfasserin aut Cristian Campos verfasserin aut Alicia Rodriguez verfasserin aut Romina Vargas verfasserin aut Thea Magrone verfasserin aut Luis A. Videla verfasserin aut In Molecules MDPI AG, 2003 25(2020), 19, p 4433 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:25 year:2020 number:19, p 4433 https://doi.org/10.3390/molecules25194433 kostenfrei https://doaj.org/article/c12b117db0054d87a0bba256d250d9a8 kostenfrei https://www.mdpi.com/1420-3049/25/19/4433 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2020 19, p 4433
language	English
source	In Molecules 25(2020), 19, p 4433 volume:25 year:2020 number:19, p 4433
sourceStr	In Molecules 25(2020), 19, p 4433 volume:25 year:2020 number:19, p 4433
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	high-fat diet white adipose tissue adipocyte hypertrophy metabolic dysfunction eicosapentaenoic acid hydroxytyrosol Organic chemistry
isfreeaccess_bool	true
container_title	Molecules
authorswithroles_txt_mv	Paola Illesca @@aut@@ Rodrigo Valenzuela @@aut@@ Alejandra Espinosa @@aut@@ Francisca Echeverría @@aut@@ Sandra Soto-Alarcon @@aut@@ Cristian Campos @@aut@@ Alicia Rodriguez @@aut@@ Romina Vargas @@aut@@ Thea Magrone @@aut@@ Luis A. Videla @@aut@@
publishDateDaySort_date	2020-01-01T00:00:00Z
hierarchy_top_id	311313132
id	DOAJ074337475
language_de	englisch
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In this study, we have hypothesized that the metabolic modulator eicosapentaenoic acid (EPA) combined with the antioxidant hydroxytyrosol (HT) attenuates HFD-induced white adipose tissue (WAT) alterations. Methods: C57BL/6J mice were administered with a HFD (60% fat, 20% protein, 20% carbohydrates) or control diet (CD; 10% fat, 20% protein, 70% carbohydrates), with or without EPA (50 mg/kg/day), HT (5 mg/kg/day), or both for 12 weeks. Determinations in WAT include morphological parameters, EPA and docosahexaenoic acid content in phospholipids (gas chromatography), lipogenesis, oxidative stress (OS) and inflammation markers, and gene expression and activities of transcription factors, such as sterol regulatory element-binding protein-1c (SREBP-1c), peroxisome proliferator-activated receptor-gamma (PPAR-γ), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) (p65 subunit) and nuclear factor erythroid 2-related factor 2 (Nrf2) (quantitative polymerase chain reaction and enzyme linked immunosorbent assay). Results: HFD led to WAT hypertrophy in relation to PPAR-γ downregulation. WAT metabolic dysfunction was characterized by upregulation of lipogenic SREBP-1c system, mitochondrial energy metabolism depression, loss of the antioxidant Nrf2 signaling with OS enhancement, n-3 long-chain polyunsaturated fatty acids depletion and activation of the pro-inflammatory NF-κB system. EPA and HT co-supplementation diminished HFD-dependent effects additively, reaching values close or similar to controls. 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callnumber-first	Q - Science
author	Paola Illesca
spellingShingle	Paola Illesca misc QD241-441 misc high-fat diet misc white adipose tissue misc adipocyte hypertrophy misc metabolic dysfunction misc eicosapentaenoic acid misc hydroxytyrosol misc Organic chemistry Protective Effects of Eicosapentaenoic Acid Plus Hydroxytyrosol Supplementation Against White Adipose Tissue Abnormalities in Mice Fed a High-Fat Diet
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topic_title	QD241-441 Protective Effects of Eicosapentaenoic Acid Plus Hydroxytyrosol Supplementation Against White Adipose Tissue Abnormalities in Mice Fed a High-Fat Diet high-fat diet white adipose tissue adipocyte hypertrophy metabolic dysfunction eicosapentaenoic acid hydroxytyrosol
topic	misc QD241-441 misc high-fat diet misc white adipose tissue misc adipocyte hypertrophy misc metabolic dysfunction misc eicosapentaenoic acid misc hydroxytyrosol misc Organic chemistry
topic_unstemmed	misc QD241-441 misc high-fat diet misc white adipose tissue misc adipocyte hypertrophy misc metabolic dysfunction misc eicosapentaenoic acid misc hydroxytyrosol misc Organic chemistry
topic_browse	misc QD241-441 misc high-fat diet misc white adipose tissue misc adipocyte hypertrophy misc metabolic dysfunction misc eicosapentaenoic acid misc hydroxytyrosol misc Organic chemistry
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title	Protective Effects of Eicosapentaenoic Acid Plus Hydroxytyrosol Supplementation Against White Adipose Tissue Abnormalities in Mice Fed a High-Fat Diet
ctrlnum	(DE-627)DOAJ074337475 (DE-599)DOAJc12b117db0054d87a0bba256d250d9a8
title_full	Protective Effects of Eicosapentaenoic Acid Plus Hydroxytyrosol Supplementation Against White Adipose Tissue Abnormalities in Mice Fed a High-Fat Diet
author_sort	Paola Illesca
journal	Molecules
journalStr	Molecules
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author_browse	Paola Illesca Rodrigo Valenzuela Alejandra Espinosa Francisca Echeverría Sandra Soto-Alarcon Cristian Campos Alicia Rodriguez Romina Vargas Thea Magrone Luis A. Videla
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doi_str_mv	10.3390/molecules25194433
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title_sort	protective effects of eicosapentaenoic acid plus hydroxytyrosol supplementation against white adipose tissue abnormalities in mice fed a high-fat diet
callnumber	QD241-441
title_auth	Protective Effects of Eicosapentaenoic Acid Plus Hydroxytyrosol Supplementation Against White Adipose Tissue Abnormalities in Mice Fed a High-Fat Diet
abstract	Objective: Obesity induced by high-fat diet (HFD) elicits white adipose tissue dysfunction. In this study, we have hypothesized that the metabolic modulator eicosapentaenoic acid (EPA) combined with the antioxidant hydroxytyrosol (HT) attenuates HFD-induced white adipose tissue (WAT) alterations. Methods: C57BL/6J mice were administered with a HFD (60% fat, 20% protein, 20% carbohydrates) or control diet (CD; 10% fat, 20% protein, 70% carbohydrates), with or without EPA (50 mg/kg/day), HT (5 mg/kg/day), or both for 12 weeks. Determinations in WAT include morphological parameters, EPA and docosahexaenoic acid content in phospholipids (gas chromatography), lipogenesis, oxidative stress (OS) and inflammation markers, and gene expression and activities of transcription factors, such as sterol regulatory element-binding protein-1c (SREBP-1c), peroxisome proliferator-activated receptor-gamma (PPAR-γ), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) (p65 subunit) and nuclear factor erythroid 2-related factor 2 (Nrf2) (quantitative polymerase chain reaction and enzyme linked immunosorbent assay). Results: HFD led to WAT hypertrophy in relation to PPAR-γ downregulation. WAT metabolic dysfunction was characterized by upregulation of lipogenic SREBP-1c system, mitochondrial energy metabolism depression, loss of the antioxidant Nrf2 signaling with OS enhancement, n-3 long-chain polyunsaturated fatty acids depletion and activation of the pro-inflammatory NF-κB system. EPA and HT co-supplementation diminished HFD-dependent effects additively, reaching values close or similar to controls. Conclusion: Data presented strengthen the importance of combined protocols such as EPA plus HT to attenuate metabolic-inflammatory states triggered by obesity.
abstractGer	Objective: Obesity induced by high-fat diet (HFD) elicits white adipose tissue dysfunction. In this study, we have hypothesized that the metabolic modulator eicosapentaenoic acid (EPA) combined with the antioxidant hydroxytyrosol (HT) attenuates HFD-induced white adipose tissue (WAT) alterations. Methods: C57BL/6J mice were administered with a HFD (60% fat, 20% protein, 20% carbohydrates) or control diet (CD; 10% fat, 20% protein, 70% carbohydrates), with or without EPA (50 mg/kg/day), HT (5 mg/kg/day), or both for 12 weeks. Determinations in WAT include morphological parameters, EPA and docosahexaenoic acid content in phospholipids (gas chromatography), lipogenesis, oxidative stress (OS) and inflammation markers, and gene expression and activities of transcription factors, such as sterol regulatory element-binding protein-1c (SREBP-1c), peroxisome proliferator-activated receptor-gamma (PPAR-γ), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) (p65 subunit) and nuclear factor erythroid 2-related factor 2 (Nrf2) (quantitative polymerase chain reaction and enzyme linked immunosorbent assay). Results: HFD led to WAT hypertrophy in relation to PPAR-γ downregulation. WAT metabolic dysfunction was characterized by upregulation of lipogenic SREBP-1c system, mitochondrial energy metabolism depression, loss of the antioxidant Nrf2 signaling with OS enhancement, n-3 long-chain polyunsaturated fatty acids depletion and activation of the pro-inflammatory NF-κB system. EPA and HT co-supplementation diminished HFD-dependent effects additively, reaching values close or similar to controls. Conclusion: Data presented strengthen the importance of combined protocols such as EPA plus HT to attenuate metabolic-inflammatory states triggered by obesity.
abstract_unstemmed	Objective: Obesity induced by high-fat diet (HFD) elicits white adipose tissue dysfunction. In this study, we have hypothesized that the metabolic modulator eicosapentaenoic acid (EPA) combined with the antioxidant hydroxytyrosol (HT) attenuates HFD-induced white adipose tissue (WAT) alterations. Methods: C57BL/6J mice were administered with a HFD (60% fat, 20% protein, 20% carbohydrates) or control diet (CD; 10% fat, 20% protein, 70% carbohydrates), with or without EPA (50 mg/kg/day), HT (5 mg/kg/day), or both for 12 weeks. Determinations in WAT include morphological parameters, EPA and docosahexaenoic acid content in phospholipids (gas chromatography), lipogenesis, oxidative stress (OS) and inflammation markers, and gene expression and activities of transcription factors, such as sterol regulatory element-binding protein-1c (SREBP-1c), peroxisome proliferator-activated receptor-gamma (PPAR-γ), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) (p65 subunit) and nuclear factor erythroid 2-related factor 2 (Nrf2) (quantitative polymerase chain reaction and enzyme linked immunosorbent assay). Results: HFD led to WAT hypertrophy in relation to PPAR-γ downregulation. WAT metabolic dysfunction was characterized by upregulation of lipogenic SREBP-1c system, mitochondrial energy metabolism depression, loss of the antioxidant Nrf2 signaling with OS enhancement, n-3 long-chain polyunsaturated fatty acids depletion and activation of the pro-inflammatory NF-κB system. EPA and HT co-supplementation diminished HFD-dependent effects additively, reaching values close or similar to controls. Conclusion: Data presented strengthen the importance of combined protocols such as EPA plus HT to attenuate metabolic-inflammatory states triggered by obesity.
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container_issue	19, p 4433
title_short	Protective Effects of Eicosapentaenoic Acid Plus Hydroxytyrosol Supplementation Against White Adipose Tissue Abnormalities in Mice Fed a High-Fat Diet
url	https://doi.org/10.3390/molecules25194433 https://doaj.org/article/c12b117db0054d87a0bba256d250d9a8 https://www.mdpi.com/1420-3049/25/19/4433 https://doaj.org/toc/1420-3049
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author2	Rodrigo Valenzuela Alejandra Espinosa Francisca Echeverría Sandra Soto-Alarcon Cristian Campos Alicia Rodriguez Romina Vargas Thea Magrone Luis A. Videla
author2Str	Rodrigo Valenzuela Alejandra Espinosa Francisca Echeverría Sandra Soto-Alarcon Cristian Campos Alicia Rodriguez Romina Vargas Thea Magrone Luis A. Videla
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doi_str	10.3390/molecules25194433
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up_date	2024-07-03T22:36:09.358Z
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Protective Effects of Eicosapentaenoic Acid Plus Hydroxytyrosol Supplementation Against White Adipose Tissue Abnormalities in Mice Fed a High-Fat Diet

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