Development of a Hadal Microbial In Situ Multi-Stage Filtering and Preserving Device

The unique environment of the hadal zone has created material circulation patterns and biological gene characteristics. Microbes play an irreplaceable role in the ocean ecological environment and material circulation due to their pervasiveness, abundance, and metabolic diversity. In this paper, we d...
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Autor*in:	Dongrui Ruan [verfasserIn] Jiawang Chen [verfasserIn] Hao Wang [verfasserIn] Xiaoqing Peng [verfasserIn] Peng Zhou [verfasserIn] Weitao He [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	hadal in situ multi-stage membrane microorganisms hadal microbial samplers

Übergeordnetes Werk:	In: Journal of Marine Science and Engineering - MDPI AG, 2014, 9(2021), 12, p 1424
Übergeordnetes Werk:	volume:9 ; year:2021 ; number:12, p 1424

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/jmse9121424

Katalog-ID:	DOAJ074360027

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callnumber-first	V - Naval Science
author	Dongrui Ruan
spellingShingle	Dongrui Ruan misc VM1-989 misc GC1-1581 misc hadal misc in situ misc multi-stage membrane misc microorganisms misc hadal microbial samplers misc Naval architecture. Shipbuilding. Marine engineering misc Oceanography Development of a Hadal Microbial In Situ Multi-Stage Filtering and Preserving Device
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topic_title	VM1-989 GC1-1581 Development of a Hadal Microbial In Situ Multi-Stage Filtering and Preserving Device hadal in situ multi-stage membrane microorganisms hadal microbial samplers
topic	misc VM1-989 misc GC1-1581 misc hadal misc in situ misc multi-stage membrane misc microorganisms misc hadal microbial samplers misc Naval architecture. Shipbuilding. Marine engineering misc Oceanography
topic_unstemmed	misc VM1-989 misc GC1-1581 misc hadal misc in situ misc multi-stage membrane misc microorganisms misc hadal microbial samplers misc Naval architecture. Shipbuilding. Marine engineering misc Oceanography
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title	Development of a Hadal Microbial In Situ Multi-Stage Filtering and Preserving Device
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title_full	Development of a Hadal Microbial In Situ Multi-Stage Filtering and Preserving Device
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author_browse	Dongrui Ruan Jiawang Chen Hao Wang Xiaoqing Peng Peng Zhou Weitao He
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title_sort	development of a hadal microbial in situ multi-stage filtering and preserving device
callnumber	VM1-989
title_auth	Development of a Hadal Microbial In Situ Multi-Stage Filtering and Preserving Device
abstract	The unique environment of the hadal zone has created material circulation patterns and biological gene characteristics. Microbes play an irreplaceable role in the ocean ecological environment and material circulation due to their pervasiveness, abundance, and metabolic diversity. In this paper, we designed and developed a microbial sampling device that can be used in a depth of 10,000 m, with its working parts suitable for the full-sea depth. The multi-stage membrane realized the in situ multi-stage filtrations. The samples were in situ fixedly preserved by RNAlater storage solution. At the same time, we modeled and calculated the multi-stage membrane separation and filtration process, simulated the interception phenomenon of particles with different sizes passing through the multi-stage membrane area, and explored the influence of varying inlet velocities. A multi-stage membrane separation and filtration test system was built. The operational characteristics of different filters were compared and analyzed, and the appropriate filter material was selected according to the flow capacity and physical properties. A 100 MPa high-pressure test was carried out to check the device’s performance under a high-pressure environment. The sampler prototype was constructed and tested in the Mariana Trench. The results indicated that the device could work at the deepest point of the Mariana trench.
abstractGer	The unique environment of the hadal zone has created material circulation patterns and biological gene characteristics. Microbes play an irreplaceable role in the ocean ecological environment and material circulation due to their pervasiveness, abundance, and metabolic diversity. In this paper, we designed and developed a microbial sampling device that can be used in a depth of 10,000 m, with its working parts suitable for the full-sea depth. The multi-stage membrane realized the in situ multi-stage filtrations. The samples were in situ fixedly preserved by RNAlater storage solution. At the same time, we modeled and calculated the multi-stage membrane separation and filtration process, simulated the interception phenomenon of particles with different sizes passing through the multi-stage membrane area, and explored the influence of varying inlet velocities. A multi-stage membrane separation and filtration test system was built. The operational characteristics of different filters were compared and analyzed, and the appropriate filter material was selected according to the flow capacity and physical properties. A 100 MPa high-pressure test was carried out to check the device’s performance under a high-pressure environment. The sampler prototype was constructed and tested in the Mariana Trench. The results indicated that the device could work at the deepest point of the Mariana trench.
abstract_unstemmed	The unique environment of the hadal zone has created material circulation patterns and biological gene characteristics. Microbes play an irreplaceable role in the ocean ecological environment and material circulation due to their pervasiveness, abundance, and metabolic diversity. In this paper, we designed and developed a microbial sampling device that can be used in a depth of 10,000 m, with its working parts suitable for the full-sea depth. The multi-stage membrane realized the in situ multi-stage filtrations. The samples were in situ fixedly preserved by RNAlater storage solution. At the same time, we modeled and calculated the multi-stage membrane separation and filtration process, simulated the interception phenomenon of particles with different sizes passing through the multi-stage membrane area, and explored the influence of varying inlet velocities. A multi-stage membrane separation and filtration test system was built. The operational characteristics of different filters were compared and analyzed, and the appropriate filter material was selected according to the flow capacity and physical properties. A 100 MPa high-pressure test was carried out to check the device’s performance under a high-pressure environment. The sampler prototype was constructed and tested in the Mariana Trench. The results indicated that the device could work at the deepest point of the Mariana trench.
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title_short	Development of a Hadal Microbial In Situ Multi-Stage Filtering and Preserving Device
url	https://doi.org/10.3390/jmse9121424 https://doaj.org/article/94c148ffb79f48db9b2a54f1a0a77559 https://www.mdpi.com/2077-1312/9/12/1424 https://doaj.org/toc/2077-1312
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author2	Jiawang Chen Hao Wang Xiaoqing Peng Peng Zhou Weitao He
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Development of a Hadal Microbial In Situ Multi-Stage Filtering and Preserving Device

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