Construction of a niche-specific spinal white matter-like tissue to promote directional axon regeneration and myelination for rat spinal cord injury repair

Directional axon regeneration and remyelination are crucial for repair of spinal cord injury (SCI), but existing treatments do not effectively promote those processes. Here, we propose a strategy for construction of niche-specific spinal white matter-like tissue (WMLT) using decellularized optic ner...
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Autor*in:	Bi-Qin Lai [verfasserIn] Yu-Rong Bai [verfasserIn] Wei-Tao Han [verfasserIn] Bao Zhang [verfasserIn] Shu Liu [verfasserIn] Jia-Hui Sun [verfasserIn] Jia-Lin Liu [verfasserIn] Ge Li [verfasserIn] Xiang Zeng [verfasserIn] Ying Ding [verfasserIn] Yuan-Huan Ma [verfasserIn] Ling Zhang [verfasserIn] Zheng-Hong Chen [verfasserIn] Jun Wang [verfasserIn] Yuan Xiong [verfasserIn] Jin-Hua Wu [verfasserIn] Qi Quan [verfasserIn] Ling-Yan Xing [verfasserIn] Hong-Bo Zhang [verfasserIn] Yuan-Shan Zeng [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	Extracellular matrix Oligodendroglial lineage cells White matter-like tissue Directional axon regeneration Spinal cord injury

Übergeordnetes Werk:	In: Bioactive Materials - KeAi Communications Co., Ltd., 2017, 11(2022), Seite 15-31
Übergeordnetes Werk:	volume:11 ; year:2022 ; pages:15-31

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.bioactmat.2021.10.005

Katalog-ID:	DOAJ074443518

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allfields	10.1016/j.bioactmat.2021.10.005 doi (DE-627)DOAJ074443518 (DE-599)DOAJ9dcc3b8d01874b4eae6399fdb019bd83 DE-627 ger DE-627 rakwb eng TA401-492 QH301-705.5 Bi-Qin Lai verfasserin aut Construction of a niche-specific spinal white matter-like tissue to promote directional axon regeneration and myelination for rat spinal cord injury repair 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Directional axon regeneration and remyelination are crucial for repair of spinal cord injury (SCI), but existing treatments do not effectively promote those processes. Here, we propose a strategy for construction of niche-specific spinal white matter-like tissue (WMLT) using decellularized optic nerve (DON) loaded with neurotrophin-3 (NT-3)-overexpressing oligodendrocyte precursor cells. A rat model with a white matter defect in the dorsal spinal cord of the T10 segment was used. The WMLT transplantation group showed significant improvement in coordinated motor functions compared with the control groups. WMLT transplants integrated well with host spinal cord white matter, effectively addressing several barriers to directional axonal regeneration and myelination during SCI repair. In WMLT, laminin was found to promote development of oligodendroglial lineage (OL) cells by binding to laminin receptors. Interestingly, laminin could also guide linear axon regeneration via interactions with specific integrins on the axon surface. The WMLT developed here utilizes the unique microstructure and bioactive matrix of DON to create a niche rich in laminin, NT-3 and OL cells to achieve significant structural repair of SCI. Our protocol can help to promote research on repair of nerve injury and construction of neural tissues and organoids that form specific cell niches. Extracellular matrix Oligodendroglial lineage cells White matter-like tissue Directional axon regeneration Spinal cord injury Materials of engineering and construction. Mechanics of materials Biology (General) Yu-Rong Bai verfasserin aut Wei-Tao Han verfasserin aut Bao Zhang verfasserin aut Shu Liu verfasserin aut Jia-Hui Sun verfasserin aut Jia-Lin Liu verfasserin aut Ge Li verfasserin aut Xiang Zeng verfasserin aut Ying Ding verfasserin aut Yuan-Huan Ma verfasserin aut Ling Zhang verfasserin aut Zheng-Hong Chen verfasserin aut Jun Wang verfasserin aut Yuan Xiong verfasserin aut Jin-Hua Wu verfasserin aut Qi Quan verfasserin aut Ling-Yan Xing verfasserin aut Hong-Bo Zhang verfasserin aut Yuan-Shan Zeng verfasserin aut In Bioactive Materials KeAi Communications Co., Ltd., 2017 11(2022), Seite 15-31 (DE-627)1663654956 (DE-600)2970496-0 2452199X nnns volume:11 year:2022 pages:15-31 https://doi.org/10.1016/j.bioactmat.2021.10.005 kostenfrei https://doaj.org/article/9dcc3b8d01874b4eae6399fdb019bd83 kostenfrei http://www.sciencedirect.com/science/article/pii/S2452199X21004564 kostenfrei https://doaj.org/toc/2452-199X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 15-31
spelling	10.1016/j.bioactmat.2021.10.005 doi (DE-627)DOAJ074443518 (DE-599)DOAJ9dcc3b8d01874b4eae6399fdb019bd83 DE-627 ger DE-627 rakwb eng TA401-492 QH301-705.5 Bi-Qin Lai verfasserin aut Construction of a niche-specific spinal white matter-like tissue to promote directional axon regeneration and myelination for rat spinal cord injury repair 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Directional axon regeneration and remyelination are crucial for repair of spinal cord injury (SCI), but existing treatments do not effectively promote those processes. Here, we propose a strategy for construction of niche-specific spinal white matter-like tissue (WMLT) using decellularized optic nerve (DON) loaded with neurotrophin-3 (NT-3)-overexpressing oligodendrocyte precursor cells. A rat model with a white matter defect in the dorsal spinal cord of the T10 segment was used. The WMLT transplantation group showed significant improvement in coordinated motor functions compared with the control groups. WMLT transplants integrated well with host spinal cord white matter, effectively addressing several barriers to directional axonal regeneration and myelination during SCI repair. In WMLT, laminin was found to promote development of oligodendroglial lineage (OL) cells by binding to laminin receptors. Interestingly, laminin could also guide linear axon regeneration via interactions with specific integrins on the axon surface. The WMLT developed here utilizes the unique microstructure and bioactive matrix of DON to create a niche rich in laminin, NT-3 and OL cells to achieve significant structural repair of SCI. Our protocol can help to promote research on repair of nerve injury and construction of neural tissues and organoids that form specific cell niches. Extracellular matrix Oligodendroglial lineage cells White matter-like tissue Directional axon regeneration Spinal cord injury Materials of engineering and construction. Mechanics of materials Biology (General) Yu-Rong Bai verfasserin aut Wei-Tao Han verfasserin aut Bao Zhang verfasserin aut Shu Liu verfasserin aut Jia-Hui Sun verfasserin aut Jia-Lin Liu verfasserin aut Ge Li verfasserin aut Xiang Zeng verfasserin aut Ying Ding verfasserin aut Yuan-Huan Ma verfasserin aut Ling Zhang verfasserin aut Zheng-Hong Chen verfasserin aut Jun Wang verfasserin aut Yuan Xiong verfasserin aut Jin-Hua Wu verfasserin aut Qi Quan verfasserin aut Ling-Yan Xing verfasserin aut Hong-Bo Zhang verfasserin aut Yuan-Shan Zeng verfasserin aut In Bioactive Materials KeAi Communications Co., Ltd., 2017 11(2022), Seite 15-31 (DE-627)1663654956 (DE-600)2970496-0 2452199X nnns volume:11 year:2022 pages:15-31 https://doi.org/10.1016/j.bioactmat.2021.10.005 kostenfrei https://doaj.org/article/9dcc3b8d01874b4eae6399fdb019bd83 kostenfrei http://www.sciencedirect.com/science/article/pii/S2452199X21004564 kostenfrei https://doaj.org/toc/2452-199X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 15-31
allfields_unstemmed	10.1016/j.bioactmat.2021.10.005 doi (DE-627)DOAJ074443518 (DE-599)DOAJ9dcc3b8d01874b4eae6399fdb019bd83 DE-627 ger DE-627 rakwb eng TA401-492 QH301-705.5 Bi-Qin Lai verfasserin aut Construction of a niche-specific spinal white matter-like tissue to promote directional axon regeneration and myelination for rat spinal cord injury repair 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Directional axon regeneration and remyelination are crucial for repair of spinal cord injury (SCI), but existing treatments do not effectively promote those processes. Here, we propose a strategy for construction of niche-specific spinal white matter-like tissue (WMLT) using decellularized optic nerve (DON) loaded with neurotrophin-3 (NT-3)-overexpressing oligodendrocyte precursor cells. A rat model with a white matter defect in the dorsal spinal cord of the T10 segment was used. The WMLT transplantation group showed significant improvement in coordinated motor functions compared with the control groups. WMLT transplants integrated well with host spinal cord white matter, effectively addressing several barriers to directional axonal regeneration and myelination during SCI repair. In WMLT, laminin was found to promote development of oligodendroglial lineage (OL) cells by binding to laminin receptors. Interestingly, laminin could also guide linear axon regeneration via interactions with specific integrins on the axon surface. The WMLT developed here utilizes the unique microstructure and bioactive matrix of DON to create a niche rich in laminin, NT-3 and OL cells to achieve significant structural repair of SCI. Our protocol can help to promote research on repair of nerve injury and construction of neural tissues and organoids that form specific cell niches. Extracellular matrix Oligodendroglial lineage cells White matter-like tissue Directional axon regeneration Spinal cord injury Materials of engineering and construction. Mechanics of materials Biology (General) Yu-Rong Bai verfasserin aut Wei-Tao Han verfasserin aut Bao Zhang verfasserin aut Shu Liu verfasserin aut Jia-Hui Sun verfasserin aut Jia-Lin Liu verfasserin aut Ge Li verfasserin aut Xiang Zeng verfasserin aut Ying Ding verfasserin aut Yuan-Huan Ma verfasserin aut Ling Zhang verfasserin aut Zheng-Hong Chen verfasserin aut Jun Wang verfasserin aut Yuan Xiong verfasserin aut Jin-Hua Wu verfasserin aut Qi Quan verfasserin aut Ling-Yan Xing verfasserin aut Hong-Bo Zhang verfasserin aut Yuan-Shan Zeng verfasserin aut In Bioactive Materials KeAi Communications Co., Ltd., 2017 11(2022), Seite 15-31 (DE-627)1663654956 (DE-600)2970496-0 2452199X nnns volume:11 year:2022 pages:15-31 https://doi.org/10.1016/j.bioactmat.2021.10.005 kostenfrei https://doaj.org/article/9dcc3b8d01874b4eae6399fdb019bd83 kostenfrei http://www.sciencedirect.com/science/article/pii/S2452199X21004564 kostenfrei https://doaj.org/toc/2452-199X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 15-31
allfieldsGer	10.1016/j.bioactmat.2021.10.005 doi (DE-627)DOAJ074443518 (DE-599)DOAJ9dcc3b8d01874b4eae6399fdb019bd83 DE-627 ger DE-627 rakwb eng TA401-492 QH301-705.5 Bi-Qin Lai verfasserin aut Construction of a niche-specific spinal white matter-like tissue to promote directional axon regeneration and myelination for rat spinal cord injury repair 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Directional axon regeneration and remyelination are crucial for repair of spinal cord injury (SCI), but existing treatments do not effectively promote those processes. Here, we propose a strategy for construction of niche-specific spinal white matter-like tissue (WMLT) using decellularized optic nerve (DON) loaded with neurotrophin-3 (NT-3)-overexpressing oligodendrocyte precursor cells. A rat model with a white matter defect in the dorsal spinal cord of the T10 segment was used. The WMLT transplantation group showed significant improvement in coordinated motor functions compared with the control groups. WMLT transplants integrated well with host spinal cord white matter, effectively addressing several barriers to directional axonal regeneration and myelination during SCI repair. In WMLT, laminin was found to promote development of oligodendroglial lineage (OL) cells by binding to laminin receptors. Interestingly, laminin could also guide linear axon regeneration via interactions with specific integrins on the axon surface. The WMLT developed here utilizes the unique microstructure and bioactive matrix of DON to create a niche rich in laminin, NT-3 and OL cells to achieve significant structural repair of SCI. Our protocol can help to promote research on repair of nerve injury and construction of neural tissues and organoids that form specific cell niches. Extracellular matrix Oligodendroglial lineage cells White matter-like tissue Directional axon regeneration Spinal cord injury Materials of engineering and construction. Mechanics of materials Biology (General) Yu-Rong Bai verfasserin aut Wei-Tao Han verfasserin aut Bao Zhang verfasserin aut Shu Liu verfasserin aut Jia-Hui Sun verfasserin aut Jia-Lin Liu verfasserin aut Ge Li verfasserin aut Xiang Zeng verfasserin aut Ying Ding verfasserin aut Yuan-Huan Ma verfasserin aut Ling Zhang verfasserin aut Zheng-Hong Chen verfasserin aut Jun Wang verfasserin aut Yuan Xiong verfasserin aut Jin-Hua Wu verfasserin aut Qi Quan verfasserin aut Ling-Yan Xing verfasserin aut Hong-Bo Zhang verfasserin aut Yuan-Shan Zeng verfasserin aut In Bioactive Materials KeAi Communications Co., Ltd., 2017 11(2022), Seite 15-31 (DE-627)1663654956 (DE-600)2970496-0 2452199X nnns volume:11 year:2022 pages:15-31 https://doi.org/10.1016/j.bioactmat.2021.10.005 kostenfrei https://doaj.org/article/9dcc3b8d01874b4eae6399fdb019bd83 kostenfrei http://www.sciencedirect.com/science/article/pii/S2452199X21004564 kostenfrei https://doaj.org/toc/2452-199X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 15-31
allfieldsSound	10.1016/j.bioactmat.2021.10.005 doi (DE-627)DOAJ074443518 (DE-599)DOAJ9dcc3b8d01874b4eae6399fdb019bd83 DE-627 ger DE-627 rakwb eng TA401-492 QH301-705.5 Bi-Qin Lai verfasserin aut Construction of a niche-specific spinal white matter-like tissue to promote directional axon regeneration and myelination for rat spinal cord injury repair 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Directional axon regeneration and remyelination are crucial for repair of spinal cord injury (SCI), but existing treatments do not effectively promote those processes. Here, we propose a strategy for construction of niche-specific spinal white matter-like tissue (WMLT) using decellularized optic nerve (DON) loaded with neurotrophin-3 (NT-3)-overexpressing oligodendrocyte precursor cells. A rat model with a white matter defect in the dorsal spinal cord of the T10 segment was used. The WMLT transplantation group showed significant improvement in coordinated motor functions compared with the control groups. WMLT transplants integrated well with host spinal cord white matter, effectively addressing several barriers to directional axonal regeneration and myelination during SCI repair. In WMLT, laminin was found to promote development of oligodendroglial lineage (OL) cells by binding to laminin receptors. Interestingly, laminin could also guide linear axon regeneration via interactions with specific integrins on the axon surface. The WMLT developed here utilizes the unique microstructure and bioactive matrix of DON to create a niche rich in laminin, NT-3 and OL cells to achieve significant structural repair of SCI. Our protocol can help to promote research on repair of nerve injury and construction of neural tissues and organoids that form specific cell niches. Extracellular matrix Oligodendroglial lineage cells White matter-like tissue Directional axon regeneration Spinal cord injury Materials of engineering and construction. Mechanics of materials Biology (General) Yu-Rong Bai verfasserin aut Wei-Tao Han verfasserin aut Bao Zhang verfasserin aut Shu Liu verfasserin aut Jia-Hui Sun verfasserin aut Jia-Lin Liu verfasserin aut Ge Li verfasserin aut Xiang Zeng verfasserin aut Ying Ding verfasserin aut Yuan-Huan Ma verfasserin aut Ling Zhang verfasserin aut Zheng-Hong Chen verfasserin aut Jun Wang verfasserin aut Yuan Xiong verfasserin aut Jin-Hua Wu verfasserin aut Qi Quan verfasserin aut Ling-Yan Xing verfasserin aut Hong-Bo Zhang verfasserin aut Yuan-Shan Zeng verfasserin aut In Bioactive Materials KeAi Communications Co., Ltd., 2017 11(2022), Seite 15-31 (DE-627)1663654956 (DE-600)2970496-0 2452199X nnns volume:11 year:2022 pages:15-31 https://doi.org/10.1016/j.bioactmat.2021.10.005 kostenfrei https://doaj.org/article/9dcc3b8d01874b4eae6399fdb019bd83 kostenfrei http://www.sciencedirect.com/science/article/pii/S2452199X21004564 kostenfrei https://doaj.org/toc/2452-199X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 15-31
language	English
source	In Bioactive Materials 11(2022), Seite 15-31 volume:11 year:2022 pages:15-31
sourceStr	In Bioactive Materials 11(2022), Seite 15-31 volume:11 year:2022 pages:15-31
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Extracellular matrix Oligodendroglial lineage cells White matter-like tissue Directional axon regeneration Spinal cord injury Materials of engineering and construction. Mechanics of materials Biology (General)
isfreeaccess_bool	true
container_title	Bioactive Materials
authorswithroles_txt_mv	Bi-Qin Lai @@aut@@ Yu-Rong Bai @@aut@@ Wei-Tao Han @@aut@@ Bao Zhang @@aut@@ Shu Liu @@aut@@ Jia-Hui Sun @@aut@@ Jia-Lin Liu @@aut@@ Ge Li @@aut@@ Xiang Zeng @@aut@@ Ying Ding @@aut@@ Yuan-Huan Ma @@aut@@ Ling Zhang @@aut@@ Zheng-Hong Chen @@aut@@ Jun Wang @@aut@@ Yuan Xiong @@aut@@ Jin-Hua Wu @@aut@@ Qi Quan @@aut@@ Ling-Yan Xing @@aut@@ Hong-Bo Zhang @@aut@@ Yuan-Shan Zeng @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	1663654956
id	DOAJ074443518
language_de	englisch
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callnumber-first	T - Technology
author	Bi-Qin Lai
spellingShingle	Bi-Qin Lai misc TA401-492 misc QH301-705.5 misc Extracellular matrix misc Oligodendroglial lineage cells misc White matter-like tissue misc Directional axon regeneration misc Spinal cord injury misc Materials of engineering and construction. Mechanics of materials misc Biology (General) Construction of a niche-specific spinal white matter-like tissue to promote directional axon regeneration and myelination for rat spinal cord injury repair
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topic_title	TA401-492 QH301-705.5 Construction of a niche-specific spinal white matter-like tissue to promote directional axon regeneration and myelination for rat spinal cord injury repair Extracellular matrix Oligodendroglial lineage cells White matter-like tissue Directional axon regeneration Spinal cord injury
topic	misc TA401-492 misc QH301-705.5 misc Extracellular matrix misc Oligodendroglial lineage cells misc White matter-like tissue misc Directional axon regeneration misc Spinal cord injury misc Materials of engineering and construction. Mechanics of materials misc Biology (General)
topic_unstemmed	misc TA401-492 misc QH301-705.5 misc Extracellular matrix misc Oligodendroglial lineage cells misc White matter-like tissue misc Directional axon regeneration misc Spinal cord injury misc Materials of engineering and construction. Mechanics of materials misc Biology (General)
topic_browse	misc TA401-492 misc QH301-705.5 misc Extracellular matrix misc Oligodendroglial lineage cells misc White matter-like tissue misc Directional axon regeneration misc Spinal cord injury misc Materials of engineering and construction. Mechanics of materials misc Biology (General)
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title	Construction of a niche-specific spinal white matter-like tissue to promote directional axon regeneration and myelination for rat spinal cord injury repair
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title_full	Construction of a niche-specific spinal white matter-like tissue to promote directional axon regeneration and myelination for rat spinal cord injury repair
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author_browse	Bi-Qin Lai Yu-Rong Bai Wei-Tao Han Bao Zhang Shu Liu Jia-Hui Sun Jia-Lin Liu Ge Li Xiang Zeng Ying Ding Yuan-Huan Ma Ling Zhang Zheng-Hong Chen Jun Wang Yuan Xiong Jin-Hua Wu Qi Quan Ling-Yan Xing Hong-Bo Zhang Yuan-Shan Zeng
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author-letter	Bi-Qin Lai
doi_str_mv	10.1016/j.bioactmat.2021.10.005
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title_sort	construction of a niche-specific spinal white matter-like tissue to promote directional axon regeneration and myelination for rat spinal cord injury repair
callnumber	TA401-492
title_auth	Construction of a niche-specific spinal white matter-like tissue to promote directional axon regeneration and myelination for rat spinal cord injury repair
abstract	Directional axon regeneration and remyelination are crucial for repair of spinal cord injury (SCI), but existing treatments do not effectively promote those processes. Here, we propose a strategy for construction of niche-specific spinal white matter-like tissue (WMLT) using decellularized optic nerve (DON) loaded with neurotrophin-3 (NT-3)-overexpressing oligodendrocyte precursor cells. A rat model with a white matter defect in the dorsal spinal cord of the T10 segment was used. The WMLT transplantation group showed significant improvement in coordinated motor functions compared with the control groups. WMLT transplants integrated well with host spinal cord white matter, effectively addressing several barriers to directional axonal regeneration and myelination during SCI repair. In WMLT, laminin was found to promote development of oligodendroglial lineage (OL) cells by binding to laminin receptors. Interestingly, laminin could also guide linear axon regeneration via interactions with specific integrins on the axon surface. The WMLT developed here utilizes the unique microstructure and bioactive matrix of DON to create a niche rich in laminin, NT-3 and OL cells to achieve significant structural repair of SCI. Our protocol can help to promote research on repair of nerve injury and construction of neural tissues and organoids that form specific cell niches.
abstractGer	Directional axon regeneration and remyelination are crucial for repair of spinal cord injury (SCI), but existing treatments do not effectively promote those processes. Here, we propose a strategy for construction of niche-specific spinal white matter-like tissue (WMLT) using decellularized optic nerve (DON) loaded with neurotrophin-3 (NT-3)-overexpressing oligodendrocyte precursor cells. A rat model with a white matter defect in the dorsal spinal cord of the T10 segment was used. The WMLT transplantation group showed significant improvement in coordinated motor functions compared with the control groups. WMLT transplants integrated well with host spinal cord white matter, effectively addressing several barriers to directional axonal regeneration and myelination during SCI repair. In WMLT, laminin was found to promote development of oligodendroglial lineage (OL) cells by binding to laminin receptors. Interestingly, laminin could also guide linear axon regeneration via interactions with specific integrins on the axon surface. The WMLT developed here utilizes the unique microstructure and bioactive matrix of DON to create a niche rich in laminin, NT-3 and OL cells to achieve significant structural repair of SCI. Our protocol can help to promote research on repair of nerve injury and construction of neural tissues and organoids that form specific cell niches.
abstract_unstemmed	Directional axon regeneration and remyelination are crucial for repair of spinal cord injury (SCI), but existing treatments do not effectively promote those processes. Here, we propose a strategy for construction of niche-specific spinal white matter-like tissue (WMLT) using decellularized optic nerve (DON) loaded with neurotrophin-3 (NT-3)-overexpressing oligodendrocyte precursor cells. A rat model with a white matter defect in the dorsal spinal cord of the T10 segment was used. The WMLT transplantation group showed significant improvement in coordinated motor functions compared with the control groups. WMLT transplants integrated well with host spinal cord white matter, effectively addressing several barriers to directional axonal regeneration and myelination during SCI repair. In WMLT, laminin was found to promote development of oligodendroglial lineage (OL) cells by binding to laminin receptors. Interestingly, laminin could also guide linear axon regeneration via interactions with specific integrins on the axon surface. The WMLT developed here utilizes the unique microstructure and bioactive matrix of DON to create a niche rich in laminin, NT-3 and OL cells to achieve significant structural repair of SCI. Our protocol can help to promote research on repair of nerve injury and construction of neural tissues and organoids that form specific cell niches.
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title_short	Construction of a niche-specific spinal white matter-like tissue to promote directional axon regeneration and myelination for rat spinal cord injury repair
url	https://doi.org/10.1016/j.bioactmat.2021.10.005 https://doaj.org/article/9dcc3b8d01874b4eae6399fdb019bd83 http://www.sciencedirect.com/science/article/pii/S2452199X21004564 https://doaj.org/toc/2452-199X
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author2Str	Yu-Rong Bai Wei-Tao Han Bao Zhang Shu Liu Jia-Hui Sun Jia-Lin Liu Ge Li Xiang Zeng Ying Ding Yuan-Huan Ma Ling Zhang Zheng-Hong Chen Jun Wang Yuan Xiong Jin-Hua Wu Qi Quan Ling-Yan Xing Hong-Bo Zhang Yuan-Shan Zeng
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up_date	2024-07-03T23:08:32.910Z
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Construction of a niche-specific spinal white matter-like tissue to promote directional axon regeneration and myelination for rat spinal cord injury repair

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