Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells

Human skin-derived precursors (SKP) represent a group of somatic stem/precursor cells that reside in dermal skin throughout life that harbor clinical potential. SKP have a high self-renewal capacity, the ability to differentiate into multiple cell types and low immunogenicity, rendering them key can...
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Autor*in:	Joery De Kock [verfasserIn] Robim Marcelino Rodrigues [verfasserIn] Steven Branson [verfasserIn] Lieven Verhoye [verfasserIn] Haaike Colemonts-Vroninks [verfasserIn] Matthias Rombaut [verfasserIn] Joost Boeckmans [verfasserIn] Jessie Neuckermans [verfasserIn] Sien Lequeue [verfasserIn] Karolien Buyl [verfasserIn] Makram Merimi [verfasserIn] Douaa Moussa Agha [verfasserIn] Veerle De Boe [verfasserIn] Laurence Lagneaux [verfasserIn] Philip Meuleman [verfasserIn] Tamara Vanhaecke [verfasserIn] Mehdi Najar [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	immunogenicity inflammation adult stem cells skin stem cells stem cell-microenvironment interactions cytokines

Übergeordnetes Werk:	In: Cells - MDPI AG, 2012, 9(2020), 4, p 914
Übergeordnetes Werk:	volume:9 ; year:2020 ; number:4, p 914

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/cells9040914

Katalog-ID:	DOAJ074568825

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520			\|a Human skin-derived precursors (SKP) represent a group of somatic stem/precursor cells that reside in dermal skin throughout life that harbor clinical potential. SKP have a high self-renewal capacity, the ability to differentiate into multiple cell types and low immunogenicity, rendering them key candidates for allogeneic cell-based, off-the-shelf therapy. However, potential clinical application of allogeneic SKP requires that these cells retain their therapeutic properties under all circumstances and, in particular, in the presence of an inflammation state. Therefore, in this study, we investigated the impact of pro-inflammatory stimulation on the secretome and immunosuppressive properties of SKP. We demonstrated that pro-inflammatory stimulation of SKP significantly changes their expression and the secretion profile of chemo/cytokines and growth factors. Most importantly, we observed that pro-inflammatory stimulated SKP were still able to suppress the graft-versus-host response when cotransplanted with human PBMC in severe-combined immune deficient (SCID) mice, albeit to a much lesser extent than unstimulated SKP. Altogether, this study demonstrates that an inflammatory microenvironment has a significant impact on the immunological properties of SKP. These alterations need to be taken into account when developing allogeneic SKP-based therapies.
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allfields	10.3390/cells9040914 doi (DE-627)DOAJ074568825 (DE-599)DOAJ0730a559768e4801b47abe21b6c10f1d DE-627 ger DE-627 rakwb eng QH573-671 Joery De Kock verfasserin aut Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Human skin-derived precursors (SKP) represent a group of somatic stem/precursor cells that reside in dermal skin throughout life that harbor clinical potential. SKP have a high self-renewal capacity, the ability to differentiate into multiple cell types and low immunogenicity, rendering them key candidates for allogeneic cell-based, off-the-shelf therapy. However, potential clinical application of allogeneic SKP requires that these cells retain their therapeutic properties under all circumstances and, in particular, in the presence of an inflammation state. Therefore, in this study, we investigated the impact of pro-inflammatory stimulation on the secretome and immunosuppressive properties of SKP. We demonstrated that pro-inflammatory stimulation of SKP significantly changes their expression and the secretion profile of chemo/cytokines and growth factors. Most importantly, we observed that pro-inflammatory stimulated SKP were still able to suppress the graft-versus-host response when cotransplanted with human PBMC in severe-combined immune deficient (SCID) mice, albeit to a much lesser extent than unstimulated SKP. Altogether, this study demonstrates that an inflammatory microenvironment has a significant impact on the immunological properties of SKP. These alterations need to be taken into account when developing allogeneic SKP-based therapies. immunogenicity inflammation adult stem cells skin stem cells stem cell-microenvironment interactions cytokines Cytology Robim Marcelino Rodrigues verfasserin aut Steven Branson verfasserin aut Lieven Verhoye verfasserin aut Haaike Colemonts-Vroninks verfasserin aut Matthias Rombaut verfasserin aut Joost Boeckmans verfasserin aut Jessie Neuckermans verfasserin aut Sien Lequeue verfasserin aut Karolien Buyl verfasserin aut Makram Merimi verfasserin aut Douaa Moussa Agha verfasserin aut Veerle De Boe verfasserin aut Laurence Lagneaux verfasserin aut Philip Meuleman verfasserin aut Tamara Vanhaecke verfasserin aut Mehdi Najar verfasserin aut In Cells MDPI AG, 2012 9(2020), 4, p 914 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:9 year:2020 number:4, p 914 https://doi.org/10.3390/cells9040914 kostenfrei https://doaj.org/article/0730a559768e4801b47abe21b6c10f1d kostenfrei https://www.mdpi.com/2073-4409/9/4/914 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 4, p 914
spelling	10.3390/cells9040914 doi (DE-627)DOAJ074568825 (DE-599)DOAJ0730a559768e4801b47abe21b6c10f1d DE-627 ger DE-627 rakwb eng QH573-671 Joery De Kock verfasserin aut Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Human skin-derived precursors (SKP) represent a group of somatic stem/precursor cells that reside in dermal skin throughout life that harbor clinical potential. SKP have a high self-renewal capacity, the ability to differentiate into multiple cell types and low immunogenicity, rendering them key candidates for allogeneic cell-based, off-the-shelf therapy. However, potential clinical application of allogeneic SKP requires that these cells retain their therapeutic properties under all circumstances and, in particular, in the presence of an inflammation state. Therefore, in this study, we investigated the impact of pro-inflammatory stimulation on the secretome and immunosuppressive properties of SKP. We demonstrated that pro-inflammatory stimulation of SKP significantly changes their expression and the secretion profile of chemo/cytokines and growth factors. Most importantly, we observed that pro-inflammatory stimulated SKP were still able to suppress the graft-versus-host response when cotransplanted with human PBMC in severe-combined immune deficient (SCID) mice, albeit to a much lesser extent than unstimulated SKP. Altogether, this study demonstrates that an inflammatory microenvironment has a significant impact on the immunological properties of SKP. These alterations need to be taken into account when developing allogeneic SKP-based therapies. immunogenicity inflammation adult stem cells skin stem cells stem cell-microenvironment interactions cytokines Cytology Robim Marcelino Rodrigues verfasserin aut Steven Branson verfasserin aut Lieven Verhoye verfasserin aut Haaike Colemonts-Vroninks verfasserin aut Matthias Rombaut verfasserin aut Joost Boeckmans verfasserin aut Jessie Neuckermans verfasserin aut Sien Lequeue verfasserin aut Karolien Buyl verfasserin aut Makram Merimi verfasserin aut Douaa Moussa Agha verfasserin aut Veerle De Boe verfasserin aut Laurence Lagneaux verfasserin aut Philip Meuleman verfasserin aut Tamara Vanhaecke verfasserin aut Mehdi Najar verfasserin aut In Cells MDPI AG, 2012 9(2020), 4, p 914 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:9 year:2020 number:4, p 914 https://doi.org/10.3390/cells9040914 kostenfrei https://doaj.org/article/0730a559768e4801b47abe21b6c10f1d kostenfrei https://www.mdpi.com/2073-4409/9/4/914 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 4, p 914
allfields_unstemmed	10.3390/cells9040914 doi (DE-627)DOAJ074568825 (DE-599)DOAJ0730a559768e4801b47abe21b6c10f1d DE-627 ger DE-627 rakwb eng QH573-671 Joery De Kock verfasserin aut Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Human skin-derived precursors (SKP) represent a group of somatic stem/precursor cells that reside in dermal skin throughout life that harbor clinical potential. SKP have a high self-renewal capacity, the ability to differentiate into multiple cell types and low immunogenicity, rendering them key candidates for allogeneic cell-based, off-the-shelf therapy. However, potential clinical application of allogeneic SKP requires that these cells retain their therapeutic properties under all circumstances and, in particular, in the presence of an inflammation state. Therefore, in this study, we investigated the impact of pro-inflammatory stimulation on the secretome and immunosuppressive properties of SKP. We demonstrated that pro-inflammatory stimulation of SKP significantly changes their expression and the secretion profile of chemo/cytokines and growth factors. Most importantly, we observed that pro-inflammatory stimulated SKP were still able to suppress the graft-versus-host response when cotransplanted with human PBMC in severe-combined immune deficient (SCID) mice, albeit to a much lesser extent than unstimulated SKP. Altogether, this study demonstrates that an inflammatory microenvironment has a significant impact on the immunological properties of SKP. These alterations need to be taken into account when developing allogeneic SKP-based therapies. immunogenicity inflammation adult stem cells skin stem cells stem cell-microenvironment interactions cytokines Cytology Robim Marcelino Rodrigues verfasserin aut Steven Branson verfasserin aut Lieven Verhoye verfasserin aut Haaike Colemonts-Vroninks verfasserin aut Matthias Rombaut verfasserin aut Joost Boeckmans verfasserin aut Jessie Neuckermans verfasserin aut Sien Lequeue verfasserin aut Karolien Buyl verfasserin aut Makram Merimi verfasserin aut Douaa Moussa Agha verfasserin aut Veerle De Boe verfasserin aut Laurence Lagneaux verfasserin aut Philip Meuleman verfasserin aut Tamara Vanhaecke verfasserin aut Mehdi Najar verfasserin aut In Cells MDPI AG, 2012 9(2020), 4, p 914 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:9 year:2020 number:4, p 914 https://doi.org/10.3390/cells9040914 kostenfrei https://doaj.org/article/0730a559768e4801b47abe21b6c10f1d kostenfrei https://www.mdpi.com/2073-4409/9/4/914 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 4, p 914
allfieldsGer	10.3390/cells9040914 doi (DE-627)DOAJ074568825 (DE-599)DOAJ0730a559768e4801b47abe21b6c10f1d DE-627 ger DE-627 rakwb eng QH573-671 Joery De Kock verfasserin aut Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Human skin-derived precursors (SKP) represent a group of somatic stem/precursor cells that reside in dermal skin throughout life that harbor clinical potential. SKP have a high self-renewal capacity, the ability to differentiate into multiple cell types and low immunogenicity, rendering them key candidates for allogeneic cell-based, off-the-shelf therapy. However, potential clinical application of allogeneic SKP requires that these cells retain their therapeutic properties under all circumstances and, in particular, in the presence of an inflammation state. Therefore, in this study, we investigated the impact of pro-inflammatory stimulation on the secretome and immunosuppressive properties of SKP. We demonstrated that pro-inflammatory stimulation of SKP significantly changes their expression and the secretion profile of chemo/cytokines and growth factors. Most importantly, we observed that pro-inflammatory stimulated SKP were still able to suppress the graft-versus-host response when cotransplanted with human PBMC in severe-combined immune deficient (SCID) mice, albeit to a much lesser extent than unstimulated SKP. Altogether, this study demonstrates that an inflammatory microenvironment has a significant impact on the immunological properties of SKP. These alterations need to be taken into account when developing allogeneic SKP-based therapies. immunogenicity inflammation adult stem cells skin stem cells stem cell-microenvironment interactions cytokines Cytology Robim Marcelino Rodrigues verfasserin aut Steven Branson verfasserin aut Lieven Verhoye verfasserin aut Haaike Colemonts-Vroninks verfasserin aut Matthias Rombaut verfasserin aut Joost Boeckmans verfasserin aut Jessie Neuckermans verfasserin aut Sien Lequeue verfasserin aut Karolien Buyl verfasserin aut Makram Merimi verfasserin aut Douaa Moussa Agha verfasserin aut Veerle De Boe verfasserin aut Laurence Lagneaux verfasserin aut Philip Meuleman verfasserin aut Tamara Vanhaecke verfasserin aut Mehdi Najar verfasserin aut In Cells MDPI AG, 2012 9(2020), 4, p 914 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:9 year:2020 number:4, p 914 https://doi.org/10.3390/cells9040914 kostenfrei https://doaj.org/article/0730a559768e4801b47abe21b6c10f1d kostenfrei https://www.mdpi.com/2073-4409/9/4/914 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 4, p 914
allfieldsSound	10.3390/cells9040914 doi (DE-627)DOAJ074568825 (DE-599)DOAJ0730a559768e4801b47abe21b6c10f1d DE-627 ger DE-627 rakwb eng QH573-671 Joery De Kock verfasserin aut Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Human skin-derived precursors (SKP) represent a group of somatic stem/precursor cells that reside in dermal skin throughout life that harbor clinical potential. SKP have a high self-renewal capacity, the ability to differentiate into multiple cell types and low immunogenicity, rendering them key candidates for allogeneic cell-based, off-the-shelf therapy. However, potential clinical application of allogeneic SKP requires that these cells retain their therapeutic properties under all circumstances and, in particular, in the presence of an inflammation state. Therefore, in this study, we investigated the impact of pro-inflammatory stimulation on the secretome and immunosuppressive properties of SKP. We demonstrated that pro-inflammatory stimulation of SKP significantly changes their expression and the secretion profile of chemo/cytokines and growth factors. Most importantly, we observed that pro-inflammatory stimulated SKP were still able to suppress the graft-versus-host response when cotransplanted with human PBMC in severe-combined immune deficient (SCID) mice, albeit to a much lesser extent than unstimulated SKP. Altogether, this study demonstrates that an inflammatory microenvironment has a significant impact on the immunological properties of SKP. These alterations need to be taken into account when developing allogeneic SKP-based therapies. immunogenicity inflammation adult stem cells skin stem cells stem cell-microenvironment interactions cytokines Cytology Robim Marcelino Rodrigues verfasserin aut Steven Branson verfasserin aut Lieven Verhoye verfasserin aut Haaike Colemonts-Vroninks verfasserin aut Matthias Rombaut verfasserin aut Joost Boeckmans verfasserin aut Jessie Neuckermans verfasserin aut Sien Lequeue verfasserin aut Karolien Buyl verfasserin aut Makram Merimi verfasserin aut Douaa Moussa Agha verfasserin aut Veerle De Boe verfasserin aut Laurence Lagneaux verfasserin aut Philip Meuleman verfasserin aut Tamara Vanhaecke verfasserin aut Mehdi Najar verfasserin aut In Cells MDPI AG, 2012 9(2020), 4, p 914 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:9 year:2020 number:4, p 914 https://doi.org/10.3390/cells9040914 kostenfrei https://doaj.org/article/0730a559768e4801b47abe21b6c10f1d kostenfrei https://www.mdpi.com/2073-4409/9/4/914 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 4, p 914
language	English
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author	Joery De Kock
spellingShingle	Joery De Kock misc QH573-671 misc immunogenicity misc inflammation misc adult stem cells misc skin stem cells misc stem cell-microenvironment interactions misc cytokines misc Cytology Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells
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topic_title	QH573-671 Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells immunogenicity inflammation adult stem cells skin stem cells stem cell-microenvironment interactions cytokines
topic	misc QH573-671 misc immunogenicity misc inflammation misc adult stem cells misc skin stem cells misc stem cell-microenvironment interactions misc cytokines misc Cytology
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title_auth	Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells
abstract	Human skin-derived precursors (SKP) represent a group of somatic stem/precursor cells that reside in dermal skin throughout life that harbor clinical potential. SKP have a high self-renewal capacity, the ability to differentiate into multiple cell types and low immunogenicity, rendering them key candidates for allogeneic cell-based, off-the-shelf therapy. However, potential clinical application of allogeneic SKP requires that these cells retain their therapeutic properties under all circumstances and, in particular, in the presence of an inflammation state. Therefore, in this study, we investigated the impact of pro-inflammatory stimulation on the secretome and immunosuppressive properties of SKP. We demonstrated that pro-inflammatory stimulation of SKP significantly changes their expression and the secretion profile of chemo/cytokines and growth factors. Most importantly, we observed that pro-inflammatory stimulated SKP were still able to suppress the graft-versus-host response when cotransplanted with human PBMC in severe-combined immune deficient (SCID) mice, albeit to a much lesser extent than unstimulated SKP. Altogether, this study demonstrates that an inflammatory microenvironment has a significant impact on the immunological properties of SKP. These alterations need to be taken into account when developing allogeneic SKP-based therapies.
abstractGer	Human skin-derived precursors (SKP) represent a group of somatic stem/precursor cells that reside in dermal skin throughout life that harbor clinical potential. SKP have a high self-renewal capacity, the ability to differentiate into multiple cell types and low immunogenicity, rendering them key candidates for allogeneic cell-based, off-the-shelf therapy. However, potential clinical application of allogeneic SKP requires that these cells retain their therapeutic properties under all circumstances and, in particular, in the presence of an inflammation state. Therefore, in this study, we investigated the impact of pro-inflammatory stimulation on the secretome and immunosuppressive properties of SKP. We demonstrated that pro-inflammatory stimulation of SKP significantly changes their expression and the secretion profile of chemo/cytokines and growth factors. Most importantly, we observed that pro-inflammatory stimulated SKP were still able to suppress the graft-versus-host response when cotransplanted with human PBMC in severe-combined immune deficient (SCID) mice, albeit to a much lesser extent than unstimulated SKP. Altogether, this study demonstrates that an inflammatory microenvironment has a significant impact on the immunological properties of SKP. These alterations need to be taken into account when developing allogeneic SKP-based therapies.
abstract_unstemmed	Human skin-derived precursors (SKP) represent a group of somatic stem/precursor cells that reside in dermal skin throughout life that harbor clinical potential. SKP have a high self-renewal capacity, the ability to differentiate into multiple cell types and low immunogenicity, rendering them key candidates for allogeneic cell-based, off-the-shelf therapy. However, potential clinical application of allogeneic SKP requires that these cells retain their therapeutic properties under all circumstances and, in particular, in the presence of an inflammation state. Therefore, in this study, we investigated the impact of pro-inflammatory stimulation on the secretome and immunosuppressive properties of SKP. We demonstrated that pro-inflammatory stimulation of SKP significantly changes their expression and the secretion profile of chemo/cytokines and growth factors. Most importantly, we observed that pro-inflammatory stimulated SKP were still able to suppress the graft-versus-host response when cotransplanted with human PBMC in severe-combined immune deficient (SCID) mice, albeit to a much lesser extent than unstimulated SKP. Altogether, this study demonstrates that an inflammatory microenvironment has a significant impact on the immunological properties of SKP. These alterations need to be taken into account when developing allogeneic SKP-based therapies.
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title_short	Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells
url	https://doi.org/10.3390/cells9040914 https://doaj.org/article/0730a559768e4801b47abe21b6c10f1d https://www.mdpi.com/2073-4409/9/4/914 https://doaj.org/toc/2073-4409
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author2	Robim Marcelino Rodrigues Steven Branson Lieven Verhoye Haaike Colemonts-Vroninks Matthias Rombaut Joost Boeckmans Jessie Neuckermans Sien Lequeue Karolien Buyl Makram Merimi Douaa Moussa Agha Veerle De Boe Laurence Lagneaux Philip Meuleman Tamara Vanhaecke Mehdi Najar
author2Str	Robim Marcelino Rodrigues Steven Branson Lieven Verhoye Haaike Colemonts-Vroninks Matthias Rombaut Joost Boeckmans Jessie Neuckermans Sien Lequeue Karolien Buyl Makram Merimi Douaa Moussa Agha Veerle De Boe Laurence Lagneaux Philip Meuleman Tamara Vanhaecke Mehdi Najar
ppnlink	718622081
callnumber-subject	QH - Natural History and Biology
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doi_str	10.3390/cells9040914
callnumber-a	QH573-671
up_date	2024-07-03T23:42:14.822Z
_version_	1803603290172162048
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SKP have a high self-renewal capacity, the ability to differentiate into multiple cell types and low immunogenicity, rendering them key candidates for allogeneic cell-based, off-the-shelf therapy. However, potential clinical application of allogeneic SKP requires that these cells retain their therapeutic properties under all circumstances and, in particular, in the presence of an inflammation state. Therefore, in this study, we investigated the impact of pro-inflammatory stimulation on the secretome and immunosuppressive properties of SKP. We demonstrated that pro-inflammatory stimulation of SKP significantly changes their expression and the secretion profile of chemo/cytokines and growth factors. Most importantly, we observed that pro-inflammatory stimulated SKP were still able to suppress the graft-versus-host response when cotransplanted with human PBMC in severe-combined immune deficient (SCID) mice, albeit to a much lesser extent than unstimulated SKP. 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