Effect of Helicobacter pylori, black tea and sodium bicarbonate on iron metabolism and MDCK cell survival
Background: Iron deficiency anemia is the most common nutritional disorder in the world. Diet and Helicobacter pylori infection are among the main causes of this disorder. Objective: In this study, the effect of black tea extract and sodium bicarbonate with Helicobacter pylori on the genes involved...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Dariush Hamedi Asl [verfasserIn] Babak Rahmani [verfasserIn] Taghi Naserpour Farivar [verfasserIn] Fatemeh Haj Manoochehri [verfasserIn] Amir Peymani [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Persisch |
| Erschienen: |
2018 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
In: The Journal of Qazvin University of Medical Sciences - Qazvin University of Medical Sciences & Health Services, 2015, 22(2018), 5, Seite 36-43 |
|---|---|
| Übergeordnetes Werk: |
volume:22 ; year:2018 ; number:5 ; pages:36-43 |
| Links: |
Link aufrufen |
|---|
| DOI / URN: |
10.29252/qums.22.5.36 |
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| Katalog-ID: |
DOAJ075009846 |
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| 245 | 1 | 0 | |a Effect of Helicobacter pylori, black tea and sodium bicarbonate on iron metabolism and MDCK cell survival |
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| 520 | |a Background: Iron deficiency anemia is the most common nutritional disorder in the world. Diet and Helicobacter pylori infection are among the main causes of this disorder. Objective: In this study, the effect of black tea extract and sodium bicarbonate with Helicobacter pylori on the genes involved in iron absorption and storage, as well as cell proliferation, were studied. Methods: Simultaneous cultivation of MDCK and Helicobacter pylori cell lines was performed at concentrations of 10, 20, 40 and 80 μg/ml of tea extract and 30, 40, 60 and 100 mM sodium bicarbonate at 24 and 48 hours. The effect of treatment on cell survival was investigated by trypan blue staining and expression of MYC, TFRC, FTH1, IRP2, IRP1, and NDRG1 genes by real-time PCR and analyzed by ANOVA and independent T-test. Findings: There was no significant change in the expression of the genes involved in iron metabolism under the influence of tea, sodium bicarbonate and Helicobacter pylori treatment in MDCK cell line. Upregulation MYC gene expression was observed in the presence of Helicobacter pylori after 24 hours treatment with tea extract, and sodium bicarbonate, and in the absence of Helicobacter pylori upregulation with tea extract after 48 hours (P<0.05). Also upregulation NDRG1 gene expression was seen after tea extract treatment of cells with or without Helicobacter pylori in both 24 and 48 h (P<0.05). Conclusion: Sodium bicarbonate and tea each one alone didn’t not influence iron status. This study suggests that reduction of tea intake could be served as a risk prevention strategy. | ||
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| 650 | 4 | |a Tea | |
| 650 | 4 | |a Sodium bicarbonate | |
| 650 | 4 | |a Helicobacter pylori | |
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10.29252/qums.22.5.36 doi (DE-627)DOAJ075009846 (DE-599)DOAJ4a9a28469721447e9df4ede922ca0fe5 DE-627 ger DE-627 rakwb per Dariush Hamedi Asl verfasserin aut Effect of Helicobacter pylori, black tea and sodium bicarbonate on iron metabolism and MDCK cell survival 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Iron deficiency anemia is the most common nutritional disorder in the world. Diet and Helicobacter pylori infection are among the main causes of this disorder. Objective: In this study, the effect of black tea extract and sodium bicarbonate with Helicobacter pylori on the genes involved in iron absorption and storage, as well as cell proliferation, were studied. Methods: Simultaneous cultivation of MDCK and Helicobacter pylori cell lines was performed at concentrations of 10, 20, 40 and 80 μg/ml of tea extract and 30, 40, 60 and 100 mM sodium bicarbonate at 24 and 48 hours. The effect of treatment on cell survival was investigated by trypan blue staining and expression of MYC, TFRC, FTH1, IRP2, IRP1, and NDRG1 genes by real-time PCR and analyzed by ANOVA and independent T-test. Findings: There was no significant change in the expression of the genes involved in iron metabolism under the influence of tea, sodium bicarbonate and Helicobacter pylori treatment in MDCK cell line. Upregulation MYC gene expression was observed in the presence of Helicobacter pylori after 24 hours treatment with tea extract, and sodium bicarbonate, and in the absence of Helicobacter pylori upregulation with tea extract after 48 hours (P<0.05). Also upregulation NDRG1 gene expression was seen after tea extract treatment of cells with or without Helicobacter pylori in both 24 and 48 h (P<0.05). Conclusion: Sodium bicarbonate and tea each one alone didn’t not influence iron status. This study suggests that reduction of tea intake could be served as a risk prevention strategy. Iron Tea Sodium bicarbonate Helicobacter pylori Medicine R Babak Rahmani verfasserin aut Taghi Naserpour Farivar verfasserin aut Fatemeh Haj Manoochehri verfasserin aut Amir Peymani verfasserin aut In The Journal of Qazvin University of Medical Sciences Qazvin University of Medical Sciences & Health Services, 2015 22(2018), 5, Seite 36-43 (DE-627)176059122X 22287213 nnns volume:22 year:2018 number:5 pages:36-43 https://doi.org/10.29252/qums.22.5.36 kostenfrei https://doaj.org/article/4a9a28469721447e9df4ede922ca0fe5 kostenfrei http://journal.qums.ac.ir/article-1-2710-en.html kostenfrei https://doaj.org/toc/1561-3666 Journal toc kostenfrei https://doaj.org/toc/2228-7213 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 22 2018 5 36-43 |
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10.29252/qums.22.5.36 doi (DE-627)DOAJ075009846 (DE-599)DOAJ4a9a28469721447e9df4ede922ca0fe5 DE-627 ger DE-627 rakwb per Dariush Hamedi Asl verfasserin aut Effect of Helicobacter pylori, black tea and sodium bicarbonate on iron metabolism and MDCK cell survival 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Iron deficiency anemia is the most common nutritional disorder in the world. Diet and Helicobacter pylori infection are among the main causes of this disorder. Objective: In this study, the effect of black tea extract and sodium bicarbonate with Helicobacter pylori on the genes involved in iron absorption and storage, as well as cell proliferation, were studied. Methods: Simultaneous cultivation of MDCK and Helicobacter pylori cell lines was performed at concentrations of 10, 20, 40 and 80 μg/ml of tea extract and 30, 40, 60 and 100 mM sodium bicarbonate at 24 and 48 hours. The effect of treatment on cell survival was investigated by trypan blue staining and expression of MYC, TFRC, FTH1, IRP2, IRP1, and NDRG1 genes by real-time PCR and analyzed by ANOVA and independent T-test. Findings: There was no significant change in the expression of the genes involved in iron metabolism under the influence of tea, sodium bicarbonate and Helicobacter pylori treatment in MDCK cell line. Upregulation MYC gene expression was observed in the presence of Helicobacter pylori after 24 hours treatment with tea extract, and sodium bicarbonate, and in the absence of Helicobacter pylori upregulation with tea extract after 48 hours (P<0.05). Also upregulation NDRG1 gene expression was seen after tea extract treatment of cells with or without Helicobacter pylori in both 24 and 48 h (P<0.05). Conclusion: Sodium bicarbonate and tea each one alone didn’t not influence iron status. This study suggests that reduction of tea intake could be served as a risk prevention strategy. Iron Tea Sodium bicarbonate Helicobacter pylori Medicine R Babak Rahmani verfasserin aut Taghi Naserpour Farivar verfasserin aut Fatemeh Haj Manoochehri verfasserin aut Amir Peymani verfasserin aut In The Journal of Qazvin University of Medical Sciences Qazvin University of Medical Sciences & Health Services, 2015 22(2018), 5, Seite 36-43 (DE-627)176059122X 22287213 nnns volume:22 year:2018 number:5 pages:36-43 https://doi.org/10.29252/qums.22.5.36 kostenfrei https://doaj.org/article/4a9a28469721447e9df4ede922ca0fe5 kostenfrei http://journal.qums.ac.ir/article-1-2710-en.html kostenfrei https://doaj.org/toc/1561-3666 Journal toc kostenfrei https://doaj.org/toc/2228-7213 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 22 2018 5 36-43 |
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10.29252/qums.22.5.36 doi (DE-627)DOAJ075009846 (DE-599)DOAJ4a9a28469721447e9df4ede922ca0fe5 DE-627 ger DE-627 rakwb per Dariush Hamedi Asl verfasserin aut Effect of Helicobacter pylori, black tea and sodium bicarbonate on iron metabolism and MDCK cell survival 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Iron deficiency anemia is the most common nutritional disorder in the world. Diet and Helicobacter pylori infection are among the main causes of this disorder. Objective: In this study, the effect of black tea extract and sodium bicarbonate with Helicobacter pylori on the genes involved in iron absorption and storage, as well as cell proliferation, were studied. Methods: Simultaneous cultivation of MDCK and Helicobacter pylori cell lines was performed at concentrations of 10, 20, 40 and 80 μg/ml of tea extract and 30, 40, 60 and 100 mM sodium bicarbonate at 24 and 48 hours. The effect of treatment on cell survival was investigated by trypan blue staining and expression of MYC, TFRC, FTH1, IRP2, IRP1, and NDRG1 genes by real-time PCR and analyzed by ANOVA and independent T-test. Findings: There was no significant change in the expression of the genes involved in iron metabolism under the influence of tea, sodium bicarbonate and Helicobacter pylori treatment in MDCK cell line. Upregulation MYC gene expression was observed in the presence of Helicobacter pylori after 24 hours treatment with tea extract, and sodium bicarbonate, and in the absence of Helicobacter pylori upregulation with tea extract after 48 hours (P<0.05). Also upregulation NDRG1 gene expression was seen after tea extract treatment of cells with or without Helicobacter pylori in both 24 and 48 h (P<0.05). Conclusion: Sodium bicarbonate and tea each one alone didn’t not influence iron status. This study suggests that reduction of tea intake could be served as a risk prevention strategy. Iron Tea Sodium bicarbonate Helicobacter pylori Medicine R Babak Rahmani verfasserin aut Taghi Naserpour Farivar verfasserin aut Fatemeh Haj Manoochehri verfasserin aut Amir Peymani verfasserin aut In The Journal of Qazvin University of Medical Sciences Qazvin University of Medical Sciences & Health Services, 2015 22(2018), 5, Seite 36-43 (DE-627)176059122X 22287213 nnns volume:22 year:2018 number:5 pages:36-43 https://doi.org/10.29252/qums.22.5.36 kostenfrei https://doaj.org/article/4a9a28469721447e9df4ede922ca0fe5 kostenfrei http://journal.qums.ac.ir/article-1-2710-en.html kostenfrei https://doaj.org/toc/1561-3666 Journal toc kostenfrei https://doaj.org/toc/2228-7213 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 22 2018 5 36-43 |
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10.29252/qums.22.5.36 doi (DE-627)DOAJ075009846 (DE-599)DOAJ4a9a28469721447e9df4ede922ca0fe5 DE-627 ger DE-627 rakwb per Dariush Hamedi Asl verfasserin aut Effect of Helicobacter pylori, black tea and sodium bicarbonate on iron metabolism and MDCK cell survival 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Iron deficiency anemia is the most common nutritional disorder in the world. Diet and Helicobacter pylori infection are among the main causes of this disorder. Objective: In this study, the effect of black tea extract and sodium bicarbonate with Helicobacter pylori on the genes involved in iron absorption and storage, as well as cell proliferation, were studied. Methods: Simultaneous cultivation of MDCK and Helicobacter pylori cell lines was performed at concentrations of 10, 20, 40 and 80 μg/ml of tea extract and 30, 40, 60 and 100 mM sodium bicarbonate at 24 and 48 hours. The effect of treatment on cell survival was investigated by trypan blue staining and expression of MYC, TFRC, FTH1, IRP2, IRP1, and NDRG1 genes by real-time PCR and analyzed by ANOVA and independent T-test. Findings: There was no significant change in the expression of the genes involved in iron metabolism under the influence of tea, sodium bicarbonate and Helicobacter pylori treatment in MDCK cell line. Upregulation MYC gene expression was observed in the presence of Helicobacter pylori after 24 hours treatment with tea extract, and sodium bicarbonate, and in the absence of Helicobacter pylori upregulation with tea extract after 48 hours (P<0.05). Also upregulation NDRG1 gene expression was seen after tea extract treatment of cells with or without Helicobacter pylori in both 24 and 48 h (P<0.05). Conclusion: Sodium bicarbonate and tea each one alone didn’t not influence iron status. This study suggests that reduction of tea intake could be served as a risk prevention strategy. Iron Tea Sodium bicarbonate Helicobacter pylori Medicine R Babak Rahmani verfasserin aut Taghi Naserpour Farivar verfasserin aut Fatemeh Haj Manoochehri verfasserin aut Amir Peymani verfasserin aut In The Journal of Qazvin University of Medical Sciences Qazvin University of Medical Sciences & Health Services, 2015 22(2018), 5, Seite 36-43 (DE-627)176059122X 22287213 nnns volume:22 year:2018 number:5 pages:36-43 https://doi.org/10.29252/qums.22.5.36 kostenfrei https://doaj.org/article/4a9a28469721447e9df4ede922ca0fe5 kostenfrei http://journal.qums.ac.ir/article-1-2710-en.html kostenfrei https://doaj.org/toc/1561-3666 Journal toc kostenfrei https://doaj.org/toc/2228-7213 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 22 2018 5 36-43 |
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10.29252/qums.22.5.36 doi (DE-627)DOAJ075009846 (DE-599)DOAJ4a9a28469721447e9df4ede922ca0fe5 DE-627 ger DE-627 rakwb per Dariush Hamedi Asl verfasserin aut Effect of Helicobacter pylori, black tea and sodium bicarbonate on iron metabolism and MDCK cell survival 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Iron deficiency anemia is the most common nutritional disorder in the world. Diet and Helicobacter pylori infection are among the main causes of this disorder. Objective: In this study, the effect of black tea extract and sodium bicarbonate with Helicobacter pylori on the genes involved in iron absorption and storage, as well as cell proliferation, were studied. Methods: Simultaneous cultivation of MDCK and Helicobacter pylori cell lines was performed at concentrations of 10, 20, 40 and 80 μg/ml of tea extract and 30, 40, 60 and 100 mM sodium bicarbonate at 24 and 48 hours. The effect of treatment on cell survival was investigated by trypan blue staining and expression of MYC, TFRC, FTH1, IRP2, IRP1, and NDRG1 genes by real-time PCR and analyzed by ANOVA and independent T-test. Findings: There was no significant change in the expression of the genes involved in iron metabolism under the influence of tea, sodium bicarbonate and Helicobacter pylori treatment in MDCK cell line. Upregulation MYC gene expression was observed in the presence of Helicobacter pylori after 24 hours treatment with tea extract, and sodium bicarbonate, and in the absence of Helicobacter pylori upregulation with tea extract after 48 hours (P<0.05). Also upregulation NDRG1 gene expression was seen after tea extract treatment of cells with or without Helicobacter pylori in both 24 and 48 h (P<0.05). Conclusion: Sodium bicarbonate and tea each one alone didn’t not influence iron status. This study suggests that reduction of tea intake could be served as a risk prevention strategy. Iron Tea Sodium bicarbonate Helicobacter pylori Medicine R Babak Rahmani verfasserin aut Taghi Naserpour Farivar verfasserin aut Fatemeh Haj Manoochehri verfasserin aut Amir Peymani verfasserin aut In The Journal of Qazvin University of Medical Sciences Qazvin University of Medical Sciences & Health Services, 2015 22(2018), 5, Seite 36-43 (DE-627)176059122X 22287213 nnns volume:22 year:2018 number:5 pages:36-43 https://doi.org/10.29252/qums.22.5.36 kostenfrei https://doaj.org/article/4a9a28469721447e9df4ede922ca0fe5 kostenfrei http://journal.qums.ac.ir/article-1-2710-en.html kostenfrei https://doaj.org/toc/1561-3666 Journal toc kostenfrei https://doaj.org/toc/2228-7213 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 22 2018 5 36-43 |
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| author |
Dariush Hamedi Asl |
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Effect of Helicobacter pylori, black tea and sodium bicarbonate on iron metabolism and MDCK cell survival Iron Tea Sodium bicarbonate Helicobacter pylori |
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Effect of Helicobacter pylori, black tea and sodium bicarbonate on iron metabolism and MDCK cell survival |
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Effect of Helicobacter pylori, black tea and sodium bicarbonate on iron metabolism and MDCK cell survival |
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Dariush Hamedi Asl |
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Dariush Hamedi Asl Babak Rahmani Taghi Naserpour Farivar Fatemeh Haj Manoochehri Amir Peymani |
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effect of helicobacter pylori, black tea and sodium bicarbonate on iron metabolism and mdck cell survival |
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Effect of Helicobacter pylori, black tea and sodium bicarbonate on iron metabolism and MDCK cell survival |
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Background: Iron deficiency anemia is the most common nutritional disorder in the world. Diet and Helicobacter pylori infection are among the main causes of this disorder. Objective: In this study, the effect of black tea extract and sodium bicarbonate with Helicobacter pylori on the genes involved in iron absorption and storage, as well as cell proliferation, were studied. Methods: Simultaneous cultivation of MDCK and Helicobacter pylori cell lines was performed at concentrations of 10, 20, 40 and 80 μg/ml of tea extract and 30, 40, 60 and 100 mM sodium bicarbonate at 24 and 48 hours. The effect of treatment on cell survival was investigated by trypan blue staining and expression of MYC, TFRC, FTH1, IRP2, IRP1, and NDRG1 genes by real-time PCR and analyzed by ANOVA and independent T-test. Findings: There was no significant change in the expression of the genes involved in iron metabolism under the influence of tea, sodium bicarbonate and Helicobacter pylori treatment in MDCK cell line. Upregulation MYC gene expression was observed in the presence of Helicobacter pylori after 24 hours treatment with tea extract, and sodium bicarbonate, and in the absence of Helicobacter pylori upregulation with tea extract after 48 hours (P<0.05). Also upregulation NDRG1 gene expression was seen after tea extract treatment of cells with or without Helicobacter pylori in both 24 and 48 h (P<0.05). Conclusion: Sodium bicarbonate and tea each one alone didn’t not influence iron status. This study suggests that reduction of tea intake could be served as a risk prevention strategy. |
| abstractGer |
Background: Iron deficiency anemia is the most common nutritional disorder in the world. Diet and Helicobacter pylori infection are among the main causes of this disorder. Objective: In this study, the effect of black tea extract and sodium bicarbonate with Helicobacter pylori on the genes involved in iron absorption and storage, as well as cell proliferation, were studied. Methods: Simultaneous cultivation of MDCK and Helicobacter pylori cell lines was performed at concentrations of 10, 20, 40 and 80 μg/ml of tea extract and 30, 40, 60 and 100 mM sodium bicarbonate at 24 and 48 hours. The effect of treatment on cell survival was investigated by trypan blue staining and expression of MYC, TFRC, FTH1, IRP2, IRP1, and NDRG1 genes by real-time PCR and analyzed by ANOVA and independent T-test. Findings: There was no significant change in the expression of the genes involved in iron metabolism under the influence of tea, sodium bicarbonate and Helicobacter pylori treatment in MDCK cell line. Upregulation MYC gene expression was observed in the presence of Helicobacter pylori after 24 hours treatment with tea extract, and sodium bicarbonate, and in the absence of Helicobacter pylori upregulation with tea extract after 48 hours (P<0.05). Also upregulation NDRG1 gene expression was seen after tea extract treatment of cells with or without Helicobacter pylori in both 24 and 48 h (P<0.05). Conclusion: Sodium bicarbonate and tea each one alone didn’t not influence iron status. This study suggests that reduction of tea intake could be served as a risk prevention strategy. |
| abstract_unstemmed |
Background: Iron deficiency anemia is the most common nutritional disorder in the world. Diet and Helicobacter pylori infection are among the main causes of this disorder. Objective: In this study, the effect of black tea extract and sodium bicarbonate with Helicobacter pylori on the genes involved in iron absorption and storage, as well as cell proliferation, were studied. Methods: Simultaneous cultivation of MDCK and Helicobacter pylori cell lines was performed at concentrations of 10, 20, 40 and 80 μg/ml of tea extract and 30, 40, 60 and 100 mM sodium bicarbonate at 24 and 48 hours. The effect of treatment on cell survival was investigated by trypan blue staining and expression of MYC, TFRC, FTH1, IRP2, IRP1, and NDRG1 genes by real-time PCR and analyzed by ANOVA and independent T-test. Findings: There was no significant change in the expression of the genes involved in iron metabolism under the influence of tea, sodium bicarbonate and Helicobacter pylori treatment in MDCK cell line. Upregulation MYC gene expression was observed in the presence of Helicobacter pylori after 24 hours treatment with tea extract, and sodium bicarbonate, and in the absence of Helicobacter pylori upregulation with tea extract after 48 hours (P<0.05). Also upregulation NDRG1 gene expression was seen after tea extract treatment of cells with or without Helicobacter pylori in both 24 and 48 h (P<0.05). Conclusion: Sodium bicarbonate and tea each one alone didn’t not influence iron status. This study suggests that reduction of tea intake could be served as a risk prevention strategy. |
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Effect of Helicobacter pylori, black tea and sodium bicarbonate on iron metabolism and MDCK cell survival |
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https://doi.org/10.29252/qums.22.5.36 https://doaj.org/article/4a9a28469721447e9df4ede922ca0fe5 http://journal.qums.ac.ir/article-1-2710-en.html https://doaj.org/toc/1561-3666 https://doaj.org/toc/2228-7213 |
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Babak Rahmani Taghi Naserpour Farivar Fatemeh Haj Manoochehri Amir Peymani |
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