Evaluation of Different Antigens in Western Blotting Technique for the Diagnosis of Sheep Haemonchosis

Background: To use different methods for serodiagnosis of ruminants' haemonchosis is important because detection of egg in the faeces is not so reliable. Methods: Peptide bands of 5 different crude antigens of intestine, uterus, cuticle, whole male and whole female of Haemonchus contortus wer...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	B Meshgi [verfasserIn] SH Hosseini [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2007

Schlagwörter:	Haemonchus contortus

Übergeordnetes Werk:	In: Iranian Journal of Parasitology - Tehran University of Medical Sciences, 2010, 2(2007), 4
Übergeordnetes Werk:	volume:2 ; year:2007 ; number:4

Links:	Link aufrufen Link aufrufen Journal toc Journal toc

Katalog-ID:	DOAJ075061880

Internformat


LEADER	01000caa a22002652 4500
001	DOAJ075061880
003	DE-627
005	20230309131943.0
007	cr uuu---uuuuu
008	230228s2007 xx \|\|\|\|\|o 00\| \|\|eng c
035			\|a (DE-627)DOAJ075061880
035			\|a (DE-599)DOAJ325b4477304741bd85cf905296a015a4
040			\|a DE-627 \|b ger \|c DE-627 \|e rakwb
041			\|a eng
050		0	\|a RC109-216
100	0		\|a B Meshgi \|e verfasserin \|4 aut
245	1	0	\|a Evaluation of Different Antigens in Western Blotting Technique for the Diagnosis of Sheep Haemonchosis
264		1	\|c 2007
336			\|a Text \|b txt \|2 rdacontent
337			\|a Computermedien \|b c \|2 rdamedia
338			\|a Online-Ressource \|b cr \|2 rdacarrier
520			\|a Background: To use different methods for serodiagnosis of ruminants' haemonchosis is important because detection of egg in the faeces is not so reliable. Methods: Peptide bands of 5 different crude antigens of intestine, uterus, cuticle, whole male and whole female of Haemonchus contortus were determined using SDS-PAGE and western blotting. Five lambs were infected with 10,000 third stage larvae of H.contortus and 2 parasites free were kept as control. Positive and negative sera collected from infected and no infected animal were tested using western blotting for immunodiagnostic antigens. Results: In electrophoresis the major peptide bands of crud antigens of uterus, intestine, cuticle, whole male and whole female of H. contortus were 7, 2, 6, 8 and 5, respectively at molecular weights of 15 to 110 kDa. In immunoblotting positive and negative sera were compared and the molecular weight of specific protein bands for Haemonchus in sheep was determined. Two major peptide bands belong to intestine and uterus with 35 and 40 kDa molecular weight, respectively, were specific for diagnosis of the parasite infection. Conclusion: Our findings suggest the use of these antigens with different molecular weights for immunodiagnosis of haemonchosis in sheep as a primary screening test is promising.
650		4	\|a Haemonchus contortus
653		0	\|a Infectious and parasitic diseases
700	0		\|a SH Hosseini \|e verfasserin \|4 aut
773	0	8	\|i In \|t Iranian Journal of Parasitology \|d Tehran University of Medical Sciences, 2010 \|g 2(2007), 4 \|w (DE-627)545785421 \|w (DE-600)2388453-8 \|x 2008238X \|7 nnns
773	1	8	\|g volume:2 \|g year:2007 \|g number:4
856	4	0	\|u https://doaj.org/article/325b4477304741bd85cf905296a015a4 \|z kostenfrei
856	4	0	\|u https://ijpa.tums.ac.ir/index.php/ijpa/article/view/37 \|z kostenfrei
856	4	2	\|u https://doaj.org/toc/1735-7020 \|y Journal toc \|z kostenfrei
856	4	2	\|u https://doaj.org/toc/2008-238X \|y Journal toc \|z kostenfrei
912			\|a GBV_USEFLAG_A
912			\|a SYSFLAG_A
912			\|a GBV_DOAJ
912			\|a GBV_ILN_20
912			\|a GBV_ILN_22
912			\|a GBV_ILN_23
912			\|a GBV_ILN_24
912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_95
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_151
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_206
912			\|a GBV_ILN_213
912			\|a GBV_ILN_230
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_602
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4367
912			\|a GBV_ILN_4700
951			\|a AR
952			\|d 2 \|j 2007 \|e 4

Indexfelder

author_variant	b m bm s h sh
matchkey_str	article:2008238X:2007----::vlainfifrnatgniwsenltigehiufrhdan
hierarchy_sort_str	2007
callnumber-subject-code	RC
publishDate	2007
allfields	(DE-627)DOAJ075061880 (DE-599)DOAJ325b4477304741bd85cf905296a015a4 DE-627 ger DE-627 rakwb eng RC109-216 B Meshgi verfasserin aut Evaluation of Different Antigens in Western Blotting Technique for the Diagnosis of Sheep Haemonchosis 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: To use different methods for serodiagnosis of ruminants' haemonchosis is important because detection of egg in the faeces is not so reliable. Methods: Peptide bands of 5 different crude antigens of intestine, uterus, cuticle, whole male and whole female of Haemonchus contortus were determined using SDS-PAGE and western blotting. Five lambs were infected with 10,000 third stage larvae of H.contortus and 2 parasites free were kept as control. Positive and negative sera collected from infected and no infected animal were tested using western blotting for immunodiagnostic antigens. Results: In electrophoresis the major peptide bands of crud antigens of uterus, intestine, cuticle, whole male and whole female of H. contortus were 7, 2, 6, 8 and 5, respectively at molecular weights of 15 to 110 kDa. In immunoblotting positive and negative sera were compared and the molecular weight of specific protein bands for Haemonchus in sheep was determined. Two major peptide bands belong to intestine and uterus with 35 and 40 kDa molecular weight, respectively, were specific for diagnosis of the parasite infection. Conclusion: Our findings suggest the use of these antigens with different molecular weights for immunodiagnosis of haemonchosis in sheep as a primary screening test is promising. Haemonchus contortus Infectious and parasitic diseases SH Hosseini verfasserin aut In Iranian Journal of Parasitology Tehran University of Medical Sciences, 2010 2(2007), 4 (DE-627)545785421 (DE-600)2388453-8 2008238X nnns volume:2 year:2007 number:4 https://doaj.org/article/325b4477304741bd85cf905296a015a4 kostenfrei https://ijpa.tums.ac.ir/index.php/ijpa/article/view/37 kostenfrei https://doaj.org/toc/1735-7020 Journal toc kostenfrei https://doaj.org/toc/2008-238X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2007 4
spelling	(DE-627)DOAJ075061880 (DE-599)DOAJ325b4477304741bd85cf905296a015a4 DE-627 ger DE-627 rakwb eng RC109-216 B Meshgi verfasserin aut Evaluation of Different Antigens in Western Blotting Technique for the Diagnosis of Sheep Haemonchosis 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: To use different methods for serodiagnosis of ruminants' haemonchosis is important because detection of egg in the faeces is not so reliable. Methods: Peptide bands of 5 different crude antigens of intestine, uterus, cuticle, whole male and whole female of Haemonchus contortus were determined using SDS-PAGE and western blotting. Five lambs were infected with 10,000 third stage larvae of H.contortus and 2 parasites free were kept as control. Positive and negative sera collected from infected and no infected animal were tested using western blotting for immunodiagnostic antigens. Results: In electrophoresis the major peptide bands of crud antigens of uterus, intestine, cuticle, whole male and whole female of H. contortus were 7, 2, 6, 8 and 5, respectively at molecular weights of 15 to 110 kDa. In immunoblotting positive and negative sera were compared and the molecular weight of specific protein bands for Haemonchus in sheep was determined. Two major peptide bands belong to intestine and uterus with 35 and 40 kDa molecular weight, respectively, were specific for diagnosis of the parasite infection. Conclusion: Our findings suggest the use of these antigens with different molecular weights for immunodiagnosis of haemonchosis in sheep as a primary screening test is promising. Haemonchus contortus Infectious and parasitic diseases SH Hosseini verfasserin aut In Iranian Journal of Parasitology Tehran University of Medical Sciences, 2010 2(2007), 4 (DE-627)545785421 (DE-600)2388453-8 2008238X nnns volume:2 year:2007 number:4 https://doaj.org/article/325b4477304741bd85cf905296a015a4 kostenfrei https://ijpa.tums.ac.ir/index.php/ijpa/article/view/37 kostenfrei https://doaj.org/toc/1735-7020 Journal toc kostenfrei https://doaj.org/toc/2008-238X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2007 4
allfields_unstemmed	(DE-627)DOAJ075061880 (DE-599)DOAJ325b4477304741bd85cf905296a015a4 DE-627 ger DE-627 rakwb eng RC109-216 B Meshgi verfasserin aut Evaluation of Different Antigens in Western Blotting Technique for the Diagnosis of Sheep Haemonchosis 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: To use different methods for serodiagnosis of ruminants' haemonchosis is important because detection of egg in the faeces is not so reliable. Methods: Peptide bands of 5 different crude antigens of intestine, uterus, cuticle, whole male and whole female of Haemonchus contortus were determined using SDS-PAGE and western blotting. Five lambs were infected with 10,000 third stage larvae of H.contortus and 2 parasites free were kept as control. Positive and negative sera collected from infected and no infected animal were tested using western blotting for immunodiagnostic antigens. Results: In electrophoresis the major peptide bands of crud antigens of uterus, intestine, cuticle, whole male and whole female of H. contortus were 7, 2, 6, 8 and 5, respectively at molecular weights of 15 to 110 kDa. In immunoblotting positive and negative sera were compared and the molecular weight of specific protein bands for Haemonchus in sheep was determined. Two major peptide bands belong to intestine and uterus with 35 and 40 kDa molecular weight, respectively, were specific for diagnosis of the parasite infection. Conclusion: Our findings suggest the use of these antigens with different molecular weights for immunodiagnosis of haemonchosis in sheep as a primary screening test is promising. Haemonchus contortus Infectious and parasitic diseases SH Hosseini verfasserin aut In Iranian Journal of Parasitology Tehran University of Medical Sciences, 2010 2(2007), 4 (DE-627)545785421 (DE-600)2388453-8 2008238X nnns volume:2 year:2007 number:4 https://doaj.org/article/325b4477304741bd85cf905296a015a4 kostenfrei https://ijpa.tums.ac.ir/index.php/ijpa/article/view/37 kostenfrei https://doaj.org/toc/1735-7020 Journal toc kostenfrei https://doaj.org/toc/2008-238X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2007 4
allfieldsGer	(DE-627)DOAJ075061880 (DE-599)DOAJ325b4477304741bd85cf905296a015a4 DE-627 ger DE-627 rakwb eng RC109-216 B Meshgi verfasserin aut Evaluation of Different Antigens in Western Blotting Technique for the Diagnosis of Sheep Haemonchosis 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: To use different methods for serodiagnosis of ruminants' haemonchosis is important because detection of egg in the faeces is not so reliable. Methods: Peptide bands of 5 different crude antigens of intestine, uterus, cuticle, whole male and whole female of Haemonchus contortus were determined using SDS-PAGE and western blotting. Five lambs were infected with 10,000 third stage larvae of H.contortus and 2 parasites free were kept as control. Positive and negative sera collected from infected and no infected animal were tested using western blotting for immunodiagnostic antigens. Results: In electrophoresis the major peptide bands of crud antigens of uterus, intestine, cuticle, whole male and whole female of H. contortus were 7, 2, 6, 8 and 5, respectively at molecular weights of 15 to 110 kDa. In immunoblotting positive and negative sera were compared and the molecular weight of specific protein bands for Haemonchus in sheep was determined. Two major peptide bands belong to intestine and uterus with 35 and 40 kDa molecular weight, respectively, were specific for diagnosis of the parasite infection. Conclusion: Our findings suggest the use of these antigens with different molecular weights for immunodiagnosis of haemonchosis in sheep as a primary screening test is promising. Haemonchus contortus Infectious and parasitic diseases SH Hosseini verfasserin aut In Iranian Journal of Parasitology Tehran University of Medical Sciences, 2010 2(2007), 4 (DE-627)545785421 (DE-600)2388453-8 2008238X nnns volume:2 year:2007 number:4 https://doaj.org/article/325b4477304741bd85cf905296a015a4 kostenfrei https://ijpa.tums.ac.ir/index.php/ijpa/article/view/37 kostenfrei https://doaj.org/toc/1735-7020 Journal toc kostenfrei https://doaj.org/toc/2008-238X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2007 4
allfieldsSound	(DE-627)DOAJ075061880 (DE-599)DOAJ325b4477304741bd85cf905296a015a4 DE-627 ger DE-627 rakwb eng RC109-216 B Meshgi verfasserin aut Evaluation of Different Antigens in Western Blotting Technique for the Diagnosis of Sheep Haemonchosis 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: To use different methods for serodiagnosis of ruminants' haemonchosis is important because detection of egg in the faeces is not so reliable. Methods: Peptide bands of 5 different crude antigens of intestine, uterus, cuticle, whole male and whole female of Haemonchus contortus were determined using SDS-PAGE and western blotting. Five lambs were infected with 10,000 third stage larvae of H.contortus and 2 parasites free were kept as control. Positive and negative sera collected from infected and no infected animal were tested using western blotting for immunodiagnostic antigens. Results: In electrophoresis the major peptide bands of crud antigens of uterus, intestine, cuticle, whole male and whole female of H. contortus were 7, 2, 6, 8 and 5, respectively at molecular weights of 15 to 110 kDa. In immunoblotting positive and negative sera were compared and the molecular weight of specific protein bands for Haemonchus in sheep was determined. Two major peptide bands belong to intestine and uterus with 35 and 40 kDa molecular weight, respectively, were specific for diagnosis of the parasite infection. Conclusion: Our findings suggest the use of these antigens with different molecular weights for immunodiagnosis of haemonchosis in sheep as a primary screening test is promising. Haemonchus contortus Infectious and parasitic diseases SH Hosseini verfasserin aut In Iranian Journal of Parasitology Tehran University of Medical Sciences, 2010 2(2007), 4 (DE-627)545785421 (DE-600)2388453-8 2008238X nnns volume:2 year:2007 number:4 https://doaj.org/article/325b4477304741bd85cf905296a015a4 kostenfrei https://ijpa.tums.ac.ir/index.php/ijpa/article/view/37 kostenfrei https://doaj.org/toc/1735-7020 Journal toc kostenfrei https://doaj.org/toc/2008-238X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2007 4
language	English
source	In Iranian Journal of Parasitology 2(2007), 4 volume:2 year:2007 number:4
sourceStr	In Iranian Journal of Parasitology 2(2007), 4 volume:2 year:2007 number:4
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Haemonchus contortus Infectious and parasitic diseases
isfreeaccess_bool	true
container_title	Iranian Journal of Parasitology
authorswithroles_txt_mv	B Meshgi @@aut@@ SH Hosseini @@aut@@
publishDateDaySort_date	2007-01-01T00:00:00Z
hierarchy_top_id	545785421
id	DOAJ075061880
language_de	englisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ075061880</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230309131943.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230228s2007 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ075061880</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ325b4477304741bd85cf905296a015a4</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">RC109-216</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">B Meshgi</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Evaluation of Different Antigens in Western Blotting Technique for the Diagnosis of Sheep Haemonchosis</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2007</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Background: To use different methods for serodiagnosis of ruminants' haemonchosis is important because detection of egg in the faeces is not so reliable. Methods: Peptide bands of 5 different crude antigens of intestine, uterus, cuticle, whole male and whole female of Haemonchus contortus were determined using SDS-PAGE and western blotting. Five lambs were infected with 10,000 third stage larvae of H.contortus and 2 parasites free were kept as control. Positive and negative sera collected from infected and no infected animal were tested using western blotting for immunodiagnostic antigens. Results: In electrophoresis the major peptide bands of crud antigens of uterus, intestine, cuticle, whole male and whole female of H. contortus were 7, 2, 6, 8 and 5, respectively at molecular weights of 15 to 110 kDa. In immunoblotting positive and negative sera were compared and the molecular weight of specific protein bands for Haemonchus in sheep was determined. Two major peptide bands belong to intestine and uterus with 35 and 40 kDa molecular weight, respectively, were specific for diagnosis of the parasite infection. Conclusion: Our findings suggest the use of these antigens with different molecular weights for immunodiagnosis of haemonchosis in sheep as a primary screening test is promising.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Haemonchus contortus</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Infectious and parasitic diseases</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">SH Hosseini</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Iranian Journal of Parasitology</subfield><subfield code="d">Tehran University of Medical Sciences, 2010</subfield><subfield code="g">2(2007), 4</subfield><subfield code="w">(DE-627)545785421</subfield><subfield code="w">(DE-600)2388453-8</subfield><subfield code="x">2008238X</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:2</subfield><subfield code="g">year:2007</subfield><subfield code="g">number:4</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/325b4477304741bd85cf905296a015a4</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://ijpa.tums.ac.ir/index.php/ijpa/article/view/37</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/1735-7020</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/2008-238X</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_206</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">2</subfield><subfield code="j">2007</subfield><subfield code="e">4</subfield></datafield></record></collection>
callnumber-first	R - Medicine
author	B Meshgi
spellingShingle	B Meshgi misc RC109-216 misc Haemonchus contortus misc Infectious and parasitic diseases Evaluation of Different Antigens in Western Blotting Technique for the Diagnosis of Sheep Haemonchosis
authorStr	B Meshgi
ppnlink_with_tag_str_mv	@@773@@(DE-627)545785421
format	electronic Article
delete_txt_mv	keep
author_role	aut aut
collection	DOAJ
remote_str	true
callnumber-label	RC109-216
illustrated	Not Illustrated
issn	2008238X
topic_title	RC109-216 Evaluation of Different Antigens in Western Blotting Technique for the Diagnosis of Sheep Haemonchosis Haemonchus contortus
topic	misc RC109-216 misc Haemonchus contortus misc Infectious and parasitic diseases
topic_unstemmed	misc RC109-216 misc Haemonchus contortus misc Infectious and parasitic diseases
topic_browse	misc RC109-216 misc Haemonchus contortus misc Infectious and parasitic diseases
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
format_main_str_mv	Text Zeitschrift/Artikel
carriertype_str_mv	cr
hierarchy_parent_title	Iranian Journal of Parasitology
hierarchy_parent_id	545785421
hierarchy_top_title	Iranian Journal of Parasitology
isfreeaccess_txt	true
familylinks_str_mv	(DE-627)545785421 (DE-600)2388453-8
title	Evaluation of Different Antigens in Western Blotting Technique for the Diagnosis of Sheep Haemonchosis
ctrlnum	(DE-627)DOAJ075061880 (DE-599)DOAJ325b4477304741bd85cf905296a015a4
title_full	Evaluation of Different Antigens in Western Blotting Technique for the Diagnosis of Sheep Haemonchosis
author_sort	B Meshgi
journal	Iranian Journal of Parasitology
journalStr	Iranian Journal of Parasitology
callnumber-first-code	R
lang_code	eng
isOA_bool	true
recordtype	marc
publishDateSort	2007
contenttype_str_mv	txt
author_browse	B Meshgi SH Hosseini
container_volume	2
class	RC109-216
format_se	Elektronische Aufsätze
author-letter	B Meshgi
author2-role	verfasserin
title_sort	evaluation of different antigens in western blotting technique for the diagnosis of sheep haemonchosis
callnumber	RC109-216
title_auth	Evaluation of Different Antigens in Western Blotting Technique for the Diagnosis of Sheep Haemonchosis
abstract	Background: To use different methods for serodiagnosis of ruminants' haemonchosis is important because detection of egg in the faeces is not so reliable. Methods: Peptide bands of 5 different crude antigens of intestine, uterus, cuticle, whole male and whole female of Haemonchus contortus were determined using SDS-PAGE and western blotting. Five lambs were infected with 10,000 third stage larvae of H.contortus and 2 parasites free were kept as control. Positive and negative sera collected from infected and no infected animal were tested using western blotting for immunodiagnostic antigens. Results: In electrophoresis the major peptide bands of crud antigens of uterus, intestine, cuticle, whole male and whole female of H. contortus were 7, 2, 6, 8 and 5, respectively at molecular weights of 15 to 110 kDa. In immunoblotting positive and negative sera were compared and the molecular weight of specific protein bands for Haemonchus in sheep was determined. Two major peptide bands belong to intestine and uterus with 35 and 40 kDa molecular weight, respectively, were specific for diagnosis of the parasite infection. Conclusion: Our findings suggest the use of these antigens with different molecular weights for immunodiagnosis of haemonchosis in sheep as a primary screening test is promising.
abstractGer	Background: To use different methods for serodiagnosis of ruminants' haemonchosis is important because detection of egg in the faeces is not so reliable. Methods: Peptide bands of 5 different crude antigens of intestine, uterus, cuticle, whole male and whole female of Haemonchus contortus were determined using SDS-PAGE and western blotting. Five lambs were infected with 10,000 third stage larvae of H.contortus and 2 parasites free were kept as control. Positive and negative sera collected from infected and no infected animal were tested using western blotting for immunodiagnostic antigens. Results: In electrophoresis the major peptide bands of crud antigens of uterus, intestine, cuticle, whole male and whole female of H. contortus were 7, 2, 6, 8 and 5, respectively at molecular weights of 15 to 110 kDa. In immunoblotting positive and negative sera were compared and the molecular weight of specific protein bands for Haemonchus in sheep was determined. Two major peptide bands belong to intestine and uterus with 35 and 40 kDa molecular weight, respectively, were specific for diagnosis of the parasite infection. Conclusion: Our findings suggest the use of these antigens with different molecular weights for immunodiagnosis of haemonchosis in sheep as a primary screening test is promising.
abstract_unstemmed	Background: To use different methods for serodiagnosis of ruminants' haemonchosis is important because detection of egg in the faeces is not so reliable. Methods: Peptide bands of 5 different crude antigens of intestine, uterus, cuticle, whole male and whole female of Haemonchus contortus were determined using SDS-PAGE and western blotting. Five lambs were infected with 10,000 third stage larvae of H.contortus and 2 parasites free were kept as control. Positive and negative sera collected from infected and no infected animal were tested using western blotting for immunodiagnostic antigens. Results: In electrophoresis the major peptide bands of crud antigens of uterus, intestine, cuticle, whole male and whole female of H. contortus were 7, 2, 6, 8 and 5, respectively at molecular weights of 15 to 110 kDa. In immunoblotting positive and negative sera were compared and the molecular weight of specific protein bands for Haemonchus in sheep was determined. Two major peptide bands belong to intestine and uterus with 35 and 40 kDa molecular weight, respectively, were specific for diagnosis of the parasite infection. Conclusion: Our findings suggest the use of these antigens with different molecular weights for immunodiagnosis of haemonchosis in sheep as a primary screening test is promising.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
container_issue	4
title_short	Evaluation of Different Antigens in Western Blotting Technique for the Diagnosis of Sheep Haemonchosis
url	https://doaj.org/article/325b4477304741bd85cf905296a015a4 https://ijpa.tums.ac.ir/index.php/ijpa/article/view/37 https://doaj.org/toc/1735-7020 https://doaj.org/toc/2008-238X
remote_bool	true
author2	SH Hosseini
author2Str	SH Hosseini
ppnlink	545785421
callnumber-subject	RC - Internal Medicine
mediatype_str_mv	c
isOA_txt	true
hochschulschrift_bool	false
callnumber-a	RC109-216
up_date	2024-07-04T01:42:03.261Z
_version_	1803610827797823488
fullrecord_marcxml	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ075061880</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230309131943.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230228s2007 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ075061880</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ325b4477304741bd85cf905296a015a4</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">RC109-216</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">B Meshgi</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Evaluation of Different Antigens in Western Blotting Technique for the Diagnosis of Sheep Haemonchosis</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2007</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Background: To use different methods for serodiagnosis of ruminants' haemonchosis is important because detection of egg in the faeces is not so reliable. Methods: Peptide bands of 5 different crude antigens of intestine, uterus, cuticle, whole male and whole female of Haemonchus contortus were determined using SDS-PAGE and western blotting. Five lambs were infected with 10,000 third stage larvae of H.contortus and 2 parasites free were kept as control. Positive and negative sera collected from infected and no infected animal were tested using western blotting for immunodiagnostic antigens. Results: In electrophoresis the major peptide bands of crud antigens of uterus, intestine, cuticle, whole male and whole female of H. contortus were 7, 2, 6, 8 and 5, respectively at molecular weights of 15 to 110 kDa. In immunoblotting positive and negative sera were compared and the molecular weight of specific protein bands for Haemonchus in sheep was determined. Two major peptide bands belong to intestine and uterus with 35 and 40 kDa molecular weight, respectively, were specific for diagnosis of the parasite infection. Conclusion: Our findings suggest the use of these antigens with different molecular weights for immunodiagnosis of haemonchosis in sheep as a primary screening test is promising.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Haemonchus contortus</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Infectious and parasitic diseases</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">SH Hosseini</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Iranian Journal of Parasitology</subfield><subfield code="d">Tehran University of Medical Sciences, 2010</subfield><subfield code="g">2(2007), 4</subfield><subfield code="w">(DE-627)545785421</subfield><subfield code="w">(DE-600)2388453-8</subfield><subfield code="x">2008238X</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:2</subfield><subfield code="g">year:2007</subfield><subfield code="g">number:4</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/325b4477304741bd85cf905296a015a4</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://ijpa.tums.ac.ir/index.php/ijpa/article/view/37</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/1735-7020</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/2008-238X</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_206</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">2</subfield><subfield code="j">2007</subfield><subfield code="e">4</subfield></datafield></record></collection>
score	7.40226

Nicht das Richtige dabei?

Schreiben Sie uns!

Evaluation of Different Antigens in Western Blotting Technique for the Diagnosis of Sheep Haemonchosis

Nicht das Richtige dabei?

Zugang & Verfügbarkeit

Vorhandene Bände

Nicht das Richtige dabei?