Comprehensive Transcriptomic Analysis of Cordyceps militaris Cultivated on Germinated Soybeans

The ascomycete fungus Cordyceps militaris infects lepidopteran larvae and pupae and forms characteristic fruiting bodies. Owing to its immune-enhancing effects, the fungus has been used as a medicine. For industrial application, this fungus can be grown on geminated soybeans as an alternative protei...
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Autor*in:	Chang-Hyuk Yoo [verfasserIn] Md. Abu Sadat [verfasserIn] Wonjae Kim [verfasserIn] Tae-Sik Park [verfasserIn] Dong Ki Park [verfasserIn] Jaehyuk Choi [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	cordyceps militaris rna-seq germinated soybeans bioinformatics

Übergeordnetes Werk:	In: Mycobiology - Taylor & Francis Group, 2018, (2022), 0, Seite 11
Übergeordnetes Werk:	year:2022 ; number:0 ; pages:11

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.1080/12298093.2022.2035906

Katalog-ID:	DOAJ075090171

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authorswithroles_txt_mv	Chang-Hyuk Yoo @@aut@@ Md. Abu Sadat @@aut@@ Wonjae Kim @@aut@@ Tae-Sik Park @@aut@@ Dong Ki Park @@aut@@ Jaehyuk Choi @@aut@@
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author	Chang-Hyuk Yoo
spellingShingle	Chang-Hyuk Yoo misc QK1-989 misc cordyceps militaris misc rna-seq misc germinated soybeans misc bioinformatics misc Botany Comprehensive Transcriptomic Analysis of Cordyceps militaris Cultivated on Germinated Soybeans
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topic_title	QK1-989 Comprehensive Transcriptomic Analysis of Cordyceps militaris Cultivated on Germinated Soybeans cordyceps militaris rna-seq germinated soybeans bioinformatics
topic	misc QK1-989 misc cordyceps militaris misc rna-seq misc germinated soybeans misc bioinformatics misc Botany
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title	Comprehensive Transcriptomic Analysis of Cordyceps militaris Cultivated on Germinated Soybeans
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title_full	Comprehensive Transcriptomic Analysis of Cordyceps militaris Cultivated on Germinated Soybeans
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author_browse	Chang-Hyuk Yoo Md. Abu Sadat Wonjae Kim Tae-Sik Park Dong Ki Park Jaehyuk Choi
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title_sort	comprehensive transcriptomic analysis of cordyceps militaris cultivated on germinated soybeans
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title_auth	Comprehensive Transcriptomic Analysis of Cordyceps militaris Cultivated on Germinated Soybeans
abstract	The ascomycete fungus Cordyceps militaris infects lepidopteran larvae and pupae and forms characteristic fruiting bodies. Owing to its immune-enhancing effects, the fungus has been used as a medicine. For industrial application, this fungus can be grown on geminated soybeans as an alternative protein source. In our study, we performed a comprehensive transcriptomic analysis to identify core gene sets during C. militaris cultivation on germinated soybeans. RNA-Seq technology was applied to the fungal cultures at seven-time points (2, 4, and 7-day and 2, 3, 5, 7-week old cultures) to investigate the global transcriptomic change. We conducted a time-series analysis using a two-step regression strategy and chose 1460 significant genes and assigned them into five clusters. Characterization of each cluster based on Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases revealed that transcription profiles changed after two weeks of incubation. Gene mapping of cordycepin biosynthesis and isoflavone modification pathways also confirmed that gene expression in the early stage of GSC cultivation is important for these metabolic pathways. Our transcriptomic analysis and selected genes provided a comprehensive molecular basis for the cultivation of C. militaris on germinated soybeans.
abstractGer	The ascomycete fungus Cordyceps militaris infects lepidopteran larvae and pupae and forms characteristic fruiting bodies. Owing to its immune-enhancing effects, the fungus has been used as a medicine. For industrial application, this fungus can be grown on geminated soybeans as an alternative protein source. In our study, we performed a comprehensive transcriptomic analysis to identify core gene sets during C. militaris cultivation on germinated soybeans. RNA-Seq technology was applied to the fungal cultures at seven-time points (2, 4, and 7-day and 2, 3, 5, 7-week old cultures) to investigate the global transcriptomic change. We conducted a time-series analysis using a two-step regression strategy and chose 1460 significant genes and assigned them into five clusters. Characterization of each cluster based on Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases revealed that transcription profiles changed after two weeks of incubation. Gene mapping of cordycepin biosynthesis and isoflavone modification pathways also confirmed that gene expression in the early stage of GSC cultivation is important for these metabolic pathways. Our transcriptomic analysis and selected genes provided a comprehensive molecular basis for the cultivation of C. militaris on germinated soybeans.
abstract_unstemmed	The ascomycete fungus Cordyceps militaris infects lepidopteran larvae and pupae and forms characteristic fruiting bodies. Owing to its immune-enhancing effects, the fungus has been used as a medicine. For industrial application, this fungus can be grown on geminated soybeans as an alternative protein source. In our study, we performed a comprehensive transcriptomic analysis to identify core gene sets during C. militaris cultivation on germinated soybeans. RNA-Seq technology was applied to the fungal cultures at seven-time points (2, 4, and 7-day and 2, 3, 5, 7-week old cultures) to investigate the global transcriptomic change. We conducted a time-series analysis using a two-step regression strategy and chose 1460 significant genes and assigned them into five clusters. Characterization of each cluster based on Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases revealed that transcription profiles changed after two weeks of incubation. Gene mapping of cordycepin biosynthesis and isoflavone modification pathways also confirmed that gene expression in the early stage of GSC cultivation is important for these metabolic pathways. Our transcriptomic analysis and selected genes provided a comprehensive molecular basis for the cultivation of C. militaris on germinated soybeans.
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title_short	Comprehensive Transcriptomic Analysis of Cordyceps militaris Cultivated on Germinated Soybeans
url	https://doi.org/10.1080/12298093.2022.2035906 https://doaj.org/article/3ba3e207882f4b809a72e57dd762a022 http://dx.doi.org/10.1080/12298093.2022.2035906 https://doaj.org/toc/1229-8093 https://doaj.org/toc/2092-9323
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Comprehensive Transcriptomic Analysis of Cordyceps militaris Cultivated on Germinated Soybeans

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