Plant regeneration of Korean wild ginseng (Panax ginseng Meyer) mutant lines induced by γ-irradiation (60Co) of adventitious roots
An efficient in vitro protocol has been established for somatic embryogenesis and plantlet conversion of Korean wild ginseng (Panax ginseng Meyer). Wild-type and mutant adventitious roots derived from the ginseng produced calluses on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2,4-dic...
Ausführliche Beschreibung
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| Autor*in: |
Jun-Ying Zhang [verfasserIn] Hyeon-Jin Sun [verfasserIn] In-Ja Song [verfasserIn] Tae-Woong Bae [verfasserIn] Hong-Gyu Kang [verfasserIn] Suk-Min Ko [verfasserIn] Yong-Ik Kwon [verfasserIn] Il-Woung Kim [verfasserIn] Jaechun Lee [verfasserIn] Shin-Young Park [verfasserIn] Pyung-Ok Lim [verfasserIn] Yong Hwan Kim [verfasserIn] Hyo-Yeon Lee [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2014 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
In: Journal of Ginseng Research - Elsevier, 2016, 38(2014), 3, Seite 220-225 |
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| Übergeordnetes Werk: |
volume:38 ; year:2014 ; number:3 ; pages:220-225 |
| Links: |
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| DOI / URN: |
10.1016/j.jgr.2014.04.001 |
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| Katalog-ID: |
DOAJ075474905 |
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| 520 | |a An efficient in vitro protocol has been established for somatic embryogenesis and plantlet conversion of Korean wild ginseng (Panax ginseng Meyer). Wild-type and mutant adventitious roots derived from the ginseng produced calluses on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2,4-dichlorophenoxyacetic acid and 0.3 mg/L kinetin; 53.3% of the explants formed callus. Embryogenic callus proliferation and somatic embryo induction occurred on MS medium containing 0.5 mg/L 2,4-dichlorophenoxyacetic acid. The induced somatic embryos further developed to maturity on MS medium with 5 mg/L gibberellic acid, and 85% of them germinated. The germinated embryos were developed to shoots and elongated on MS medium with 5 mg/L gibberellic acid. The shoots developed into plants with well-developed taproots on one-third strength Schenk and Hildebrandt basal medium supplemented with 0.25 mg/L 1-naphthaleneacetic acid. When the plants were transferred to soil, about 30% of the regenerated plants developed into normal plants. | ||
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10.1016/j.jgr.2014.04.001 doi (DE-627)DOAJ075474905 (DE-599)DOAJ69e1a76c3c4f4bbcac7bdcd13c59983f DE-627 ger DE-627 rakwb eng QK1-989 Jun-Ying Zhang verfasserin aut Plant regeneration of Korean wild ginseng (Panax ginseng Meyer) mutant lines induced by γ-irradiation (60Co) of adventitious roots 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier An efficient in vitro protocol has been established for somatic embryogenesis and plantlet conversion of Korean wild ginseng (Panax ginseng Meyer). Wild-type and mutant adventitious roots derived from the ginseng produced calluses on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2,4-dichlorophenoxyacetic acid and 0.3 mg/L kinetin; 53.3% of the explants formed callus. Embryogenic callus proliferation and somatic embryo induction occurred on MS medium containing 0.5 mg/L 2,4-dichlorophenoxyacetic acid. The induced somatic embryos further developed to maturity on MS medium with 5 mg/L gibberellic acid, and 85% of them germinated. The germinated embryos were developed to shoots and elongated on MS medium with 5 mg/L gibberellic acid. The shoots developed into plants with well-developed taproots on one-third strength Schenk and Hildebrandt basal medium supplemented with 0.25 mg/L 1-naphthaleneacetic acid. When the plants were transferred to soil, about 30% of the regenerated plants developed into normal plants. mutant adventitious roots Panax ginseng Meyer somatic embryo somatic embryogenesis Botany Hyeon-Jin Sun verfasserin aut In-Ja Song verfasserin aut Tae-Woong Bae verfasserin aut Hong-Gyu Kang verfasserin aut Suk-Min Ko verfasserin aut Yong-Ik Kwon verfasserin aut Il-Woung Kim verfasserin aut Jaechun Lee verfasserin aut Shin-Young Park verfasserin aut Pyung-Ok Lim verfasserin aut Yong Hwan Kim verfasserin aut Hyo-Yeon Lee verfasserin aut In Journal of Ginseng Research Elsevier, 2016 38(2014), 3, Seite 220-225 (DE-627)1760621897 12268453 nnns volume:38 year:2014 number:3 pages:220-225 https://doi.org/10.1016/j.jgr.2014.04.001 kostenfrei https://doaj.org/article/69e1a76c3c4f4bbcac7bdcd13c59983f kostenfrei http://www.sciencedirect.com/science/article/pii/S1226845314000517 kostenfrei https://doaj.org/toc/1226-8453 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 38 2014 3 220-225 |
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10.1016/j.jgr.2014.04.001 doi (DE-627)DOAJ075474905 (DE-599)DOAJ69e1a76c3c4f4bbcac7bdcd13c59983f DE-627 ger DE-627 rakwb eng QK1-989 Jun-Ying Zhang verfasserin aut Plant regeneration of Korean wild ginseng (Panax ginseng Meyer) mutant lines induced by γ-irradiation (60Co) of adventitious roots 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier An efficient in vitro protocol has been established for somatic embryogenesis and plantlet conversion of Korean wild ginseng (Panax ginseng Meyer). Wild-type and mutant adventitious roots derived from the ginseng produced calluses on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2,4-dichlorophenoxyacetic acid and 0.3 mg/L kinetin; 53.3% of the explants formed callus. Embryogenic callus proliferation and somatic embryo induction occurred on MS medium containing 0.5 mg/L 2,4-dichlorophenoxyacetic acid. The induced somatic embryos further developed to maturity on MS medium with 5 mg/L gibberellic acid, and 85% of them germinated. The germinated embryos were developed to shoots and elongated on MS medium with 5 mg/L gibberellic acid. The shoots developed into plants with well-developed taproots on one-third strength Schenk and Hildebrandt basal medium supplemented with 0.25 mg/L 1-naphthaleneacetic acid. When the plants were transferred to soil, about 30% of the regenerated plants developed into normal plants. mutant adventitious roots Panax ginseng Meyer somatic embryo somatic embryogenesis Botany Hyeon-Jin Sun verfasserin aut In-Ja Song verfasserin aut Tae-Woong Bae verfasserin aut Hong-Gyu Kang verfasserin aut Suk-Min Ko verfasserin aut Yong-Ik Kwon verfasserin aut Il-Woung Kim verfasserin aut Jaechun Lee verfasserin aut Shin-Young Park verfasserin aut Pyung-Ok Lim verfasserin aut Yong Hwan Kim verfasserin aut Hyo-Yeon Lee verfasserin aut In Journal of Ginseng Research Elsevier, 2016 38(2014), 3, Seite 220-225 (DE-627)1760621897 12268453 nnns volume:38 year:2014 number:3 pages:220-225 https://doi.org/10.1016/j.jgr.2014.04.001 kostenfrei https://doaj.org/article/69e1a76c3c4f4bbcac7bdcd13c59983f kostenfrei http://www.sciencedirect.com/science/article/pii/S1226845314000517 kostenfrei https://doaj.org/toc/1226-8453 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 38 2014 3 220-225 |
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10.1016/j.jgr.2014.04.001 doi (DE-627)DOAJ075474905 (DE-599)DOAJ69e1a76c3c4f4bbcac7bdcd13c59983f DE-627 ger DE-627 rakwb eng QK1-989 Jun-Ying Zhang verfasserin aut Plant regeneration of Korean wild ginseng (Panax ginseng Meyer) mutant lines induced by γ-irradiation (60Co) of adventitious roots 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier An efficient in vitro protocol has been established for somatic embryogenesis and plantlet conversion of Korean wild ginseng (Panax ginseng Meyer). Wild-type and mutant adventitious roots derived from the ginseng produced calluses on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2,4-dichlorophenoxyacetic acid and 0.3 mg/L kinetin; 53.3% of the explants formed callus. Embryogenic callus proliferation and somatic embryo induction occurred on MS medium containing 0.5 mg/L 2,4-dichlorophenoxyacetic acid. The induced somatic embryos further developed to maturity on MS medium with 5 mg/L gibberellic acid, and 85% of them germinated. The germinated embryos were developed to shoots and elongated on MS medium with 5 mg/L gibberellic acid. The shoots developed into plants with well-developed taproots on one-third strength Schenk and Hildebrandt basal medium supplemented with 0.25 mg/L 1-naphthaleneacetic acid. When the plants were transferred to soil, about 30% of the regenerated plants developed into normal plants. mutant adventitious roots Panax ginseng Meyer somatic embryo somatic embryogenesis Botany Hyeon-Jin Sun verfasserin aut In-Ja Song verfasserin aut Tae-Woong Bae verfasserin aut Hong-Gyu Kang verfasserin aut Suk-Min Ko verfasserin aut Yong-Ik Kwon verfasserin aut Il-Woung Kim verfasserin aut Jaechun Lee verfasserin aut Shin-Young Park verfasserin aut Pyung-Ok Lim verfasserin aut Yong Hwan Kim verfasserin aut Hyo-Yeon Lee verfasserin aut In Journal of Ginseng Research Elsevier, 2016 38(2014), 3, Seite 220-225 (DE-627)1760621897 12268453 nnns volume:38 year:2014 number:3 pages:220-225 https://doi.org/10.1016/j.jgr.2014.04.001 kostenfrei https://doaj.org/article/69e1a76c3c4f4bbcac7bdcd13c59983f kostenfrei http://www.sciencedirect.com/science/article/pii/S1226845314000517 kostenfrei https://doaj.org/toc/1226-8453 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 38 2014 3 220-225 |
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10.1016/j.jgr.2014.04.001 doi (DE-627)DOAJ075474905 (DE-599)DOAJ69e1a76c3c4f4bbcac7bdcd13c59983f DE-627 ger DE-627 rakwb eng QK1-989 Jun-Ying Zhang verfasserin aut Plant regeneration of Korean wild ginseng (Panax ginseng Meyer) mutant lines induced by γ-irradiation (60Co) of adventitious roots 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier An efficient in vitro protocol has been established for somatic embryogenesis and plantlet conversion of Korean wild ginseng (Panax ginseng Meyer). Wild-type and mutant adventitious roots derived from the ginseng produced calluses on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2,4-dichlorophenoxyacetic acid and 0.3 mg/L kinetin; 53.3% of the explants formed callus. Embryogenic callus proliferation and somatic embryo induction occurred on MS medium containing 0.5 mg/L 2,4-dichlorophenoxyacetic acid. The induced somatic embryos further developed to maturity on MS medium with 5 mg/L gibberellic acid, and 85% of them germinated. The germinated embryos were developed to shoots and elongated on MS medium with 5 mg/L gibberellic acid. The shoots developed into plants with well-developed taproots on one-third strength Schenk and Hildebrandt basal medium supplemented with 0.25 mg/L 1-naphthaleneacetic acid. When the plants were transferred to soil, about 30% of the regenerated plants developed into normal plants. mutant adventitious roots Panax ginseng Meyer somatic embryo somatic embryogenesis Botany Hyeon-Jin Sun verfasserin aut In-Ja Song verfasserin aut Tae-Woong Bae verfasserin aut Hong-Gyu Kang verfasserin aut Suk-Min Ko verfasserin aut Yong-Ik Kwon verfasserin aut Il-Woung Kim verfasserin aut Jaechun Lee verfasserin aut Shin-Young Park verfasserin aut Pyung-Ok Lim verfasserin aut Yong Hwan Kim verfasserin aut Hyo-Yeon Lee verfasserin aut In Journal of Ginseng Research Elsevier, 2016 38(2014), 3, Seite 220-225 (DE-627)1760621897 12268453 nnns volume:38 year:2014 number:3 pages:220-225 https://doi.org/10.1016/j.jgr.2014.04.001 kostenfrei https://doaj.org/article/69e1a76c3c4f4bbcac7bdcd13c59983f kostenfrei http://www.sciencedirect.com/science/article/pii/S1226845314000517 kostenfrei https://doaj.org/toc/1226-8453 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 38 2014 3 220-225 |
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10.1016/j.jgr.2014.04.001 doi (DE-627)DOAJ075474905 (DE-599)DOAJ69e1a76c3c4f4bbcac7bdcd13c59983f DE-627 ger DE-627 rakwb eng QK1-989 Jun-Ying Zhang verfasserin aut Plant regeneration of Korean wild ginseng (Panax ginseng Meyer) mutant lines induced by γ-irradiation (60Co) of adventitious roots 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier An efficient in vitro protocol has been established for somatic embryogenesis and plantlet conversion of Korean wild ginseng (Panax ginseng Meyer). Wild-type and mutant adventitious roots derived from the ginseng produced calluses on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2,4-dichlorophenoxyacetic acid and 0.3 mg/L kinetin; 53.3% of the explants formed callus. Embryogenic callus proliferation and somatic embryo induction occurred on MS medium containing 0.5 mg/L 2,4-dichlorophenoxyacetic acid. The induced somatic embryos further developed to maturity on MS medium with 5 mg/L gibberellic acid, and 85% of them germinated. The germinated embryos were developed to shoots and elongated on MS medium with 5 mg/L gibberellic acid. The shoots developed into plants with well-developed taproots on one-third strength Schenk and Hildebrandt basal medium supplemented with 0.25 mg/L 1-naphthaleneacetic acid. When the plants were transferred to soil, about 30% of the regenerated plants developed into normal plants. mutant adventitious roots Panax ginseng Meyer somatic embryo somatic embryogenesis Botany Hyeon-Jin Sun verfasserin aut In-Ja Song verfasserin aut Tae-Woong Bae verfasserin aut Hong-Gyu Kang verfasserin aut Suk-Min Ko verfasserin aut Yong-Ik Kwon verfasserin aut Il-Woung Kim verfasserin aut Jaechun Lee verfasserin aut Shin-Young Park verfasserin aut Pyung-Ok Lim verfasserin aut Yong Hwan Kim verfasserin aut Hyo-Yeon Lee verfasserin aut In Journal of Ginseng Research Elsevier, 2016 38(2014), 3, Seite 220-225 (DE-627)1760621897 12268453 nnns volume:38 year:2014 number:3 pages:220-225 https://doi.org/10.1016/j.jgr.2014.04.001 kostenfrei https://doaj.org/article/69e1a76c3c4f4bbcac7bdcd13c59983f kostenfrei http://www.sciencedirect.com/science/article/pii/S1226845314000517 kostenfrei https://doaj.org/toc/1226-8453 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 38 2014 3 220-225 |
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Jun-Ying Zhang @@aut@@ Hyeon-Jin Sun @@aut@@ In-Ja Song @@aut@@ Tae-Woong Bae @@aut@@ Hong-Gyu Kang @@aut@@ Suk-Min Ko @@aut@@ Yong-Ik Kwon @@aut@@ Il-Woung Kim @@aut@@ Jaechun Lee @@aut@@ Shin-Young Park @@aut@@ Pyung-Ok Lim @@aut@@ Yong Hwan Kim @@aut@@ Hyo-Yeon Lee @@aut@@ |
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Plant regeneration of Korean wild ginseng (Panax ginseng Meyer) mutant lines induced by γ-irradiation (60Co) of adventitious roots |
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Jun-Ying Zhang Hyeon-Jin Sun In-Ja Song Tae-Woong Bae Hong-Gyu Kang Suk-Min Ko Yong-Ik Kwon Il-Woung Kim Jaechun Lee Shin-Young Park Pyung-Ok Lim Yong Hwan Kim Hyo-Yeon Lee |
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plant regeneration of korean wild ginseng (panax ginseng meyer) mutant lines induced by γ-irradiation (60co) of adventitious roots |
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Plant regeneration of Korean wild ginseng (Panax ginseng Meyer) mutant lines induced by γ-irradiation (60Co) of adventitious roots |
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An efficient in vitro protocol has been established for somatic embryogenesis and plantlet conversion of Korean wild ginseng (Panax ginseng Meyer). Wild-type and mutant adventitious roots derived from the ginseng produced calluses on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2,4-dichlorophenoxyacetic acid and 0.3 mg/L kinetin; 53.3% of the explants formed callus. Embryogenic callus proliferation and somatic embryo induction occurred on MS medium containing 0.5 mg/L 2,4-dichlorophenoxyacetic acid. The induced somatic embryos further developed to maturity on MS medium with 5 mg/L gibberellic acid, and 85% of them germinated. The germinated embryos were developed to shoots and elongated on MS medium with 5 mg/L gibberellic acid. The shoots developed into plants with well-developed taproots on one-third strength Schenk and Hildebrandt basal medium supplemented with 0.25 mg/L 1-naphthaleneacetic acid. When the plants were transferred to soil, about 30% of the regenerated plants developed into normal plants. |
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An efficient in vitro protocol has been established for somatic embryogenesis and plantlet conversion of Korean wild ginseng (Panax ginseng Meyer). Wild-type and mutant adventitious roots derived from the ginseng produced calluses on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2,4-dichlorophenoxyacetic acid and 0.3 mg/L kinetin; 53.3% of the explants formed callus. Embryogenic callus proliferation and somatic embryo induction occurred on MS medium containing 0.5 mg/L 2,4-dichlorophenoxyacetic acid. The induced somatic embryos further developed to maturity on MS medium with 5 mg/L gibberellic acid, and 85% of them germinated. The germinated embryos were developed to shoots and elongated on MS medium with 5 mg/L gibberellic acid. The shoots developed into plants with well-developed taproots on one-third strength Schenk and Hildebrandt basal medium supplemented with 0.25 mg/L 1-naphthaleneacetic acid. When the plants were transferred to soil, about 30% of the regenerated plants developed into normal plants. |
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An efficient in vitro protocol has been established for somatic embryogenesis and plantlet conversion of Korean wild ginseng (Panax ginseng Meyer). Wild-type and mutant adventitious roots derived from the ginseng produced calluses on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2,4-dichlorophenoxyacetic acid and 0.3 mg/L kinetin; 53.3% of the explants formed callus. Embryogenic callus proliferation and somatic embryo induction occurred on MS medium containing 0.5 mg/L 2,4-dichlorophenoxyacetic acid. The induced somatic embryos further developed to maturity on MS medium with 5 mg/L gibberellic acid, and 85% of them germinated. The germinated embryos were developed to shoots and elongated on MS medium with 5 mg/L gibberellic acid. The shoots developed into plants with well-developed taproots on one-third strength Schenk and Hildebrandt basal medium supplemented with 0.25 mg/L 1-naphthaleneacetic acid. When the plants were transferred to soil, about 30% of the regenerated plants developed into normal plants. |
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Plant regeneration of Korean wild ginseng (Panax ginseng Meyer) mutant lines induced by γ-irradiation (60Co) of adventitious roots |
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