An immunomagnetic cell separation system based on a retroviral vector containing a chimeric, recombinant human-murine CD4 gene

Primary B- and T-lymphocytes and neoplastic cells of B- and T-cell origin are particularly hard to transfect with plasmids. Thus, functional and molecular studies in immunology and oncohematology require selection of cells after plasmid transduction, assuring obtaining homogeneous cell populations e...
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Gespeichert in:

Autor*in:	Patryk Górniak [verfasserIn] Przemysław Juszczyński [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Schlagwörter:	cell selection immunomagnetic selection cell transfection CD4 immunomagnetic beads

Übergeordnetes Werk:	In: Central European Journal of Immunology - Termedia Publishing House, 2017, 43(2018), 3, Seite 353-357
Übergeordnetes Werk:	volume:43 ; year:2018 ; number:3 ; pages:353-357

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.5114/ceji.2018.80057

Katalog-ID:	DOAJ075557460

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source	In Central European Journal of Immunology 43(2018), 3, Seite 353-357 volume:43 year:2018 number:3 pages:353-357
sourceStr	In Central European Journal of Immunology 43(2018), 3, Seite 353-357 volume:43 year:2018 number:3 pages:353-357
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author	Patryk Górniak
spellingShingle	Patryk Górniak misc cell selection misc immunomagnetic selection misc cell transfection misc CD4 misc immunomagnetic beads misc Medicine misc R An immunomagnetic cell separation system based on a retroviral vector containing a chimeric, recombinant human-murine CD4 gene
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topic_title	An immunomagnetic cell separation system based on a retroviral vector containing a chimeric, recombinant human-murine CD4 gene cell selection immunomagnetic selection cell transfection CD4 immunomagnetic beads
topic	misc cell selection misc immunomagnetic selection misc cell transfection misc CD4 misc immunomagnetic beads misc Medicine misc R
topic_unstemmed	misc cell selection misc immunomagnetic selection misc cell transfection misc CD4 misc immunomagnetic beads misc Medicine misc R
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title	An immunomagnetic cell separation system based on a retroviral vector containing a chimeric, recombinant human-murine CD4 gene
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title_full	An immunomagnetic cell separation system based on a retroviral vector containing a chimeric, recombinant human-murine CD4 gene
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title_sort	immunomagnetic cell separation system based on a retroviral vector containing a chimeric, recombinant human-murine cd4 gene
title_auth	An immunomagnetic cell separation system based on a retroviral vector containing a chimeric, recombinant human-murine CD4 gene
abstract	Primary B- and T-lymphocytes and neoplastic cells of B- and T-cell origin are particularly hard to transfect with plasmids. Thus, functional and molecular studies in immunology and oncohematology require selection of cells after plasmid transduction, assuring obtaining homogeneous cell populations expressing plasmid-delivered molecules. The selection is usually achieved by incorporation of an antibiotic resistance gene or fluorescent protein into a plasmid and subsequent antibiotic selection or fluorescence-activated cell sorting (FACS). However, these cell sorting methods have significant drawbacks that limit their utilization. An ideal system should be fast, cheap and ensure efficient selection of transfected cells. In an attempt to deliver a sorting system meeting these requirements, we have generated a plasmid for retroviral gene delivery and expression, containing a recombinant human-murine CD4 gene, with a truncated intracellular domain to prevent undesired signaling events. After retroviral infection, cells expressing hmCD4 are selected by immunomagnetic anti-mCD4 beads. The system offers a very robust, efficient, fast and cost-saving cell separation solution. The vector is available for the academic community upon request.
abstractGer	Primary B- and T-lymphocytes and neoplastic cells of B- and T-cell origin are particularly hard to transfect with plasmids. Thus, functional and molecular studies in immunology and oncohematology require selection of cells after plasmid transduction, assuring obtaining homogeneous cell populations expressing plasmid-delivered molecules. The selection is usually achieved by incorporation of an antibiotic resistance gene or fluorescent protein into a plasmid and subsequent antibiotic selection or fluorescence-activated cell sorting (FACS). However, these cell sorting methods have significant drawbacks that limit their utilization. An ideal system should be fast, cheap and ensure efficient selection of transfected cells. In an attempt to deliver a sorting system meeting these requirements, we have generated a plasmid for retroviral gene delivery and expression, containing a recombinant human-murine CD4 gene, with a truncated intracellular domain to prevent undesired signaling events. After retroviral infection, cells expressing hmCD4 are selected by immunomagnetic anti-mCD4 beads. The system offers a very robust, efficient, fast and cost-saving cell separation solution. The vector is available for the academic community upon request.
abstract_unstemmed	Primary B- and T-lymphocytes and neoplastic cells of B- and T-cell origin are particularly hard to transfect with plasmids. Thus, functional and molecular studies in immunology and oncohematology require selection of cells after plasmid transduction, assuring obtaining homogeneous cell populations expressing plasmid-delivered molecules. The selection is usually achieved by incorporation of an antibiotic resistance gene or fluorescent protein into a plasmid and subsequent antibiotic selection or fluorescence-activated cell sorting (FACS). However, these cell sorting methods have significant drawbacks that limit their utilization. An ideal system should be fast, cheap and ensure efficient selection of transfected cells. In an attempt to deliver a sorting system meeting these requirements, we have generated a plasmid for retroviral gene delivery and expression, containing a recombinant human-murine CD4 gene, with a truncated intracellular domain to prevent undesired signaling events. After retroviral infection, cells expressing hmCD4 are selected by immunomagnetic anti-mCD4 beads. The system offers a very robust, efficient, fast and cost-saving cell separation solution. The vector is available for the academic community upon request.
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title_short	An immunomagnetic cell separation system based on a retroviral vector containing a chimeric, recombinant human-murine CD4 gene
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An immunomagnetic cell separation system based on a retroviral vector containing a chimeric, recombinant human-murine CD4 gene

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