Towards phasing using high X-ray intensity
X-ray free-electron lasers (XFELs) show great promise for macromolecular structure determination from sub-micrometre-sized crystals, using the emerging method of serial femtosecond crystallography. The extreme brightness of the XFEL radiation can multiply ionize most, if not all, atoms in a protein,...
Ausführliche Beschreibung
Autor*in: |
Lorenzo Galli [verfasserIn] Sang-Kil Son [verfasserIn] Thomas R. M. Barends [verfasserIn] Thomas A. White [verfasserIn] Anton Barty [verfasserIn] Sabine Botha [verfasserIn] Sébastien Boutet [verfasserIn] Carl Caleman [verfasserIn] R. Bruce Doak [verfasserIn] Max H. Nanao [verfasserIn] Karol Nass [verfasserIn] Robert L. Shoeman [verfasserIn] Nicusor Timneanu [verfasserIn] Robin Santra [verfasserIn] Ilme Schlichting [verfasserIn] Henry N. Chapman [verfasserIn] |
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E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2015 |
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Übergeordnetes Werk: |
In: IUCrJ - International Union of Crystallography, 2014, 2(2015), 6, Seite 627-634 |
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Übergeordnetes Werk: |
volume:2 ; year:2015 ; number:6 ; pages:627-634 |
Links: |
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DOI / URN: |
10.1107/S2052252515014049 |
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Katalog-ID: |
DOAJ075583232 |
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10.1107/S2052252515014049 doi (DE-627)DOAJ075583232 (DE-599)DOAJ470b97134cd24a229aa61150852a3784 DE-627 ger DE-627 rakwb eng QD901-999 Lorenzo Galli verfasserin aut Towards phasing using high X-ray intensity 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier X-ray free-electron lasers (XFELs) show great promise for macromolecular structure determination from sub-micrometre-sized crystals, using the emerging method of serial femtosecond crystallography. The extreme brightness of the XFEL radiation can multiply ionize most, if not all, atoms in a protein, causing their scattering factors to change during the pulse, with a preferential `bleaching' of heavy atoms. This paper investigates the effects of electronic damage on experimental data collected from a Gd derivative of lysozyme microcrystals at different X-ray intensities, and the degree of ionization of Gd atoms is quantified from phased difference Fourier maps. A pattern sorting scheme is proposed to maximize the ionization contrast and the way in which the local electronic damage can be used for a new experimental phasing method is discussed. serial femtosecond crystallography high-intensity phasing radiation damage electronic damage X-ray free-electron lasers high XFEL doses Crystallography Sang-Kil Son verfasserin aut Thomas R. M. Barends verfasserin aut Thomas A. White verfasserin aut Anton Barty verfasserin aut Sabine Botha verfasserin aut Sébastien Boutet verfasserin aut Carl Caleman verfasserin aut R. Bruce Doak verfasserin aut Max H. Nanao verfasserin aut Karol Nass verfasserin aut Robert L. Shoeman verfasserin aut Nicusor Timneanu verfasserin aut Robin Santra verfasserin aut Ilme Schlichting verfasserin aut Henry N. Chapman verfasserin aut In IUCrJ International Union of Crystallography, 2014 2(2015), 6, Seite 627-634 (DE-627)777782758 (DE-600)2754953-7 20522525 nnns volume:2 year:2015 number:6 pages:627-634 https://doi.org/10.1107/S2052252515014049 kostenfrei https://doaj.org/article/470b97134cd24a229aa61150852a3784 kostenfrei http://scripts.iucr.org/cgi-bin/paper?S2052252515014049 kostenfrei https://doaj.org/toc/2052-2525 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2015 6 627-634 |
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10.1107/S2052252515014049 doi (DE-627)DOAJ075583232 (DE-599)DOAJ470b97134cd24a229aa61150852a3784 DE-627 ger DE-627 rakwb eng QD901-999 Lorenzo Galli verfasserin aut Towards phasing using high X-ray intensity 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier X-ray free-electron lasers (XFELs) show great promise for macromolecular structure determination from sub-micrometre-sized crystals, using the emerging method of serial femtosecond crystallography. The extreme brightness of the XFEL radiation can multiply ionize most, if not all, atoms in a protein, causing their scattering factors to change during the pulse, with a preferential `bleaching' of heavy atoms. This paper investigates the effects of electronic damage on experimental data collected from a Gd derivative of lysozyme microcrystals at different X-ray intensities, and the degree of ionization of Gd atoms is quantified from phased difference Fourier maps. A pattern sorting scheme is proposed to maximize the ionization contrast and the way in which the local electronic damage can be used for a new experimental phasing method is discussed. serial femtosecond crystallography high-intensity phasing radiation damage electronic damage X-ray free-electron lasers high XFEL doses Crystallography Sang-Kil Son verfasserin aut Thomas R. M. Barends verfasserin aut Thomas A. White verfasserin aut Anton Barty verfasserin aut Sabine Botha verfasserin aut Sébastien Boutet verfasserin aut Carl Caleman verfasserin aut R. Bruce Doak verfasserin aut Max H. Nanao verfasserin aut Karol Nass verfasserin aut Robert L. Shoeman verfasserin aut Nicusor Timneanu verfasserin aut Robin Santra verfasserin aut Ilme Schlichting verfasserin aut Henry N. Chapman verfasserin aut In IUCrJ International Union of Crystallography, 2014 2(2015), 6, Seite 627-634 (DE-627)777782758 (DE-600)2754953-7 20522525 nnns volume:2 year:2015 number:6 pages:627-634 https://doi.org/10.1107/S2052252515014049 kostenfrei https://doaj.org/article/470b97134cd24a229aa61150852a3784 kostenfrei http://scripts.iucr.org/cgi-bin/paper?S2052252515014049 kostenfrei https://doaj.org/toc/2052-2525 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2015 6 627-634 |
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10.1107/S2052252515014049 doi (DE-627)DOAJ075583232 (DE-599)DOAJ470b97134cd24a229aa61150852a3784 DE-627 ger DE-627 rakwb eng QD901-999 Lorenzo Galli verfasserin aut Towards phasing using high X-ray intensity 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier X-ray free-electron lasers (XFELs) show great promise for macromolecular structure determination from sub-micrometre-sized crystals, using the emerging method of serial femtosecond crystallography. The extreme brightness of the XFEL radiation can multiply ionize most, if not all, atoms in a protein, causing their scattering factors to change during the pulse, with a preferential `bleaching' of heavy atoms. This paper investigates the effects of electronic damage on experimental data collected from a Gd derivative of lysozyme microcrystals at different X-ray intensities, and the degree of ionization of Gd atoms is quantified from phased difference Fourier maps. A pattern sorting scheme is proposed to maximize the ionization contrast and the way in which the local electronic damage can be used for a new experimental phasing method is discussed. serial femtosecond crystallography high-intensity phasing radiation damage electronic damage X-ray free-electron lasers high XFEL doses Crystallography Sang-Kil Son verfasserin aut Thomas R. M. Barends verfasserin aut Thomas A. White verfasserin aut Anton Barty verfasserin aut Sabine Botha verfasserin aut Sébastien Boutet verfasserin aut Carl Caleman verfasserin aut R. Bruce Doak verfasserin aut Max H. Nanao verfasserin aut Karol Nass verfasserin aut Robert L. Shoeman verfasserin aut Nicusor Timneanu verfasserin aut Robin Santra verfasserin aut Ilme Schlichting verfasserin aut Henry N. Chapman verfasserin aut In IUCrJ International Union of Crystallography, 2014 2(2015), 6, Seite 627-634 (DE-627)777782758 (DE-600)2754953-7 20522525 nnns volume:2 year:2015 number:6 pages:627-634 https://doi.org/10.1107/S2052252515014049 kostenfrei https://doaj.org/article/470b97134cd24a229aa61150852a3784 kostenfrei http://scripts.iucr.org/cgi-bin/paper?S2052252515014049 kostenfrei https://doaj.org/toc/2052-2525 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2015 6 627-634 |
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10.1107/S2052252515014049 doi (DE-627)DOAJ075583232 (DE-599)DOAJ470b97134cd24a229aa61150852a3784 DE-627 ger DE-627 rakwb eng QD901-999 Lorenzo Galli verfasserin aut Towards phasing using high X-ray intensity 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier X-ray free-electron lasers (XFELs) show great promise for macromolecular structure determination from sub-micrometre-sized crystals, using the emerging method of serial femtosecond crystallography. The extreme brightness of the XFEL radiation can multiply ionize most, if not all, atoms in a protein, causing their scattering factors to change during the pulse, with a preferential `bleaching' of heavy atoms. This paper investigates the effects of electronic damage on experimental data collected from a Gd derivative of lysozyme microcrystals at different X-ray intensities, and the degree of ionization of Gd atoms is quantified from phased difference Fourier maps. A pattern sorting scheme is proposed to maximize the ionization contrast and the way in which the local electronic damage can be used for a new experimental phasing method is discussed. serial femtosecond crystallography high-intensity phasing radiation damage electronic damage X-ray free-electron lasers high XFEL doses Crystallography Sang-Kil Son verfasserin aut Thomas R. M. Barends verfasserin aut Thomas A. White verfasserin aut Anton Barty verfasserin aut Sabine Botha verfasserin aut Sébastien Boutet verfasserin aut Carl Caleman verfasserin aut R. Bruce Doak verfasserin aut Max H. Nanao verfasserin aut Karol Nass verfasserin aut Robert L. Shoeman verfasserin aut Nicusor Timneanu verfasserin aut Robin Santra verfasserin aut Ilme Schlichting verfasserin aut Henry N. Chapman verfasserin aut In IUCrJ International Union of Crystallography, 2014 2(2015), 6, Seite 627-634 (DE-627)777782758 (DE-600)2754953-7 20522525 nnns volume:2 year:2015 number:6 pages:627-634 https://doi.org/10.1107/S2052252515014049 kostenfrei https://doaj.org/article/470b97134cd24a229aa61150852a3784 kostenfrei http://scripts.iucr.org/cgi-bin/paper?S2052252515014049 kostenfrei https://doaj.org/toc/2052-2525 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2015 6 627-634 |
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X-ray free-electron lasers (XFELs) show great promise for macromolecular structure determination from sub-micrometre-sized crystals, using the emerging method of serial femtosecond crystallography. The extreme brightness of the XFEL radiation can multiply ionize most, if not all, atoms in a protein, causing their scattering factors to change during the pulse, with a preferential `bleaching' of heavy atoms. This paper investigates the effects of electronic damage on experimental data collected from a Gd derivative of lysozyme microcrystals at different X-ray intensities, and the degree of ionization of Gd atoms is quantified from phased difference Fourier maps. A pattern sorting scheme is proposed to maximize the ionization contrast and the way in which the local electronic damage can be used for a new experimental phasing method is discussed. |
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X-ray free-electron lasers (XFELs) show great promise for macromolecular structure determination from sub-micrometre-sized crystals, using the emerging method of serial femtosecond crystallography. The extreme brightness of the XFEL radiation can multiply ionize most, if not all, atoms in a protein, causing their scattering factors to change during the pulse, with a preferential `bleaching' of heavy atoms. This paper investigates the effects of electronic damage on experimental data collected from a Gd derivative of lysozyme microcrystals at different X-ray intensities, and the degree of ionization of Gd atoms is quantified from phased difference Fourier maps. A pattern sorting scheme is proposed to maximize the ionization contrast and the way in which the local electronic damage can be used for a new experimental phasing method is discussed. |
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X-ray free-electron lasers (XFELs) show great promise for macromolecular structure determination from sub-micrometre-sized crystals, using the emerging method of serial femtosecond crystallography. The extreme brightness of the XFEL radiation can multiply ionize most, if not all, atoms in a protein, causing their scattering factors to change during the pulse, with a preferential `bleaching' of heavy atoms. This paper investigates the effects of electronic damage on experimental data collected from a Gd derivative of lysozyme microcrystals at different X-ray intensities, and the degree of ionization of Gd atoms is quantified from phased difference Fourier maps. A pattern sorting scheme is proposed to maximize the ionization contrast and the way in which the local electronic damage can be used for a new experimental phasing method is discussed. |
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