Towards phasing using high X-ray intensity

X-ray free-electron lasers (XFELs) show great promise for macromolecular structure determination from sub-micrometre-sized crystals, using the emerging method of serial femtosecond crystallography. The extreme brightness of the XFEL radiation can multiply ionize most, if not all, atoms in a protein,...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Lorenzo Galli [verfasserIn] Sang-Kil Son [verfasserIn] Thomas R. M. Barends [verfasserIn] Thomas A. White [verfasserIn] Anton Barty [verfasserIn] Sabine Botha [verfasserIn] Sébastien Boutet [verfasserIn] Carl Caleman [verfasserIn] R. Bruce Doak [verfasserIn] Max H. Nanao [verfasserIn] Karol Nass [verfasserIn] Robert L. Shoeman [verfasserIn] Nicusor Timneanu [verfasserIn] Robin Santra [verfasserIn] Ilme Schlichting [verfasserIn] Henry N. Chapman [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2015

Schlagwörter:	serial femtosecond crystallography high-intensity phasing radiation damage electronic damage X-ray free-electron lasers high XFEL doses

Übergeordnetes Werk:	In: IUCrJ - International Union of Crystallography, 2014, 2(2015), 6, Seite 627-634
Übergeordnetes Werk:	volume:2 ; year:2015 ; number:6 ; pages:627-634

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1107/S2052252515014049

Katalog-ID:	DOAJ075583232

Internformat


LEADER	01000caa a22002652 4500
001	DOAJ075583232
003	DE-627
005	20230309134713.0
007	cr uuu---uuuuu
008	230228s2015 xx \|\|\|\|\|o 00\| \|\|eng c
024	7		\|a 10.1107/S2052252515014049 \|2 doi
035			\|a (DE-627)DOAJ075583232
035			\|a (DE-599)DOAJ470b97134cd24a229aa61150852a3784
040			\|a DE-627 \|b ger \|c DE-627 \|e rakwb
041			\|a eng
050		0	\|a QD901-999
100	0		\|a Lorenzo Galli \|e verfasserin \|4 aut
245	1	0	\|a Towards phasing using high X-ray intensity
264		1	\|c 2015
336			\|a Text \|b txt \|2 rdacontent
337			\|a Computermedien \|b c \|2 rdamedia
338			\|a Online-Ressource \|b cr \|2 rdacarrier
520			\|a X-ray free-electron lasers (XFELs) show great promise for macromolecular structure determination from sub-micrometre-sized crystals, using the emerging method of serial femtosecond crystallography. The extreme brightness of the XFEL radiation can multiply ionize most, if not all, atoms in a protein, causing their scattering factors to change during the pulse, with a preferential `bleaching' of heavy atoms. This paper investigates the effects of electronic damage on experimental data collected from a Gd derivative of lysozyme microcrystals at different X-ray intensities, and the degree of ionization of Gd atoms is quantified from phased difference Fourier maps. A pattern sorting scheme is proposed to maximize the ionization contrast and the way in which the local electronic damage can be used for a new experimental phasing method is discussed.
650		4	\|a serial femtosecond crystallography
650		4	\|a high-intensity phasing
650		4	\|a radiation damage
650		4	\|a electronic damage
650		4	\|a X-ray free-electron lasers
650		4	\|a high XFEL doses
653		0	\|a Crystallography
700	0		\|a Sang-Kil Son \|e verfasserin \|4 aut
700	0		\|a Thomas R. M. Barends \|e verfasserin \|4 aut
700	0		\|a Thomas A. White \|e verfasserin \|4 aut
700	0		\|a Anton Barty \|e verfasserin \|4 aut
700	0		\|a Sabine Botha \|e verfasserin \|4 aut
700	0		\|a Sébastien Boutet \|e verfasserin \|4 aut
700	0		\|a Carl Caleman \|e verfasserin \|4 aut
700	0		\|a R. Bruce Doak \|e verfasserin \|4 aut
700	0		\|a Max H. Nanao \|e verfasserin \|4 aut
700	0		\|a Karol Nass \|e verfasserin \|4 aut
700	0		\|a Robert L. Shoeman \|e verfasserin \|4 aut
700	0		\|a Nicusor Timneanu \|e verfasserin \|4 aut
700	0		\|a Robin Santra \|e verfasserin \|4 aut
700	0		\|a Ilme Schlichting \|e verfasserin \|4 aut
700	0		\|a Henry N. Chapman \|e verfasserin \|4 aut
773	0	8	\|i In \|t IUCrJ \|d International Union of Crystallography, 2014 \|g 2(2015), 6, Seite 627-634 \|w (DE-627)777782758 \|w (DE-600)2754953-7 \|x 20522525 \|7 nnns
773	1	8	\|g volume:2 \|g year:2015 \|g number:6 \|g pages:627-634
856	4	0	\|u https://doi.org/10.1107/S2052252515014049 \|z kostenfrei
856	4	0	\|u https://doaj.org/article/470b97134cd24a229aa61150852a3784 \|z kostenfrei
856	4	0	\|u http://scripts.iucr.org/cgi-bin/paper?S2052252515014049 \|z kostenfrei
856	4	2	\|u https://doaj.org/toc/2052-2525 \|y Journal toc \|z kostenfrei
912			\|a GBV_USEFLAG_A
912			\|a SYSFLAG_A
912			\|a GBV_DOAJ
912			\|a GBV_ILN_11
912			\|a GBV_ILN_20
912			\|a GBV_ILN_22
912			\|a GBV_ILN_23
912			\|a GBV_ILN_24
912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_95
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_151
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_213
912			\|a GBV_ILN_230
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4367
912			\|a GBV_ILN_4700
951			\|a AR
952			\|d 2 \|j 2015 \|e 6 \|h 627-634

Indexfelder

author_variant	l g lg s k s sks t r m b trmb t a w taw a b ab s b sb s b sb c c cc r b d rbd m h n mhn k n kn r l s rls n t nt r s rs i s is h n c hnc
matchkey_str	article:20522525:2015----::oadpaigsnhgxa
hierarchy_sort_str	2015
callnumber-subject-code	QD
publishDate	2015
allfields	10.1107/S2052252515014049 doi (DE-627)DOAJ075583232 (DE-599)DOAJ470b97134cd24a229aa61150852a3784 DE-627 ger DE-627 rakwb eng QD901-999 Lorenzo Galli verfasserin aut Towards phasing using high X-ray intensity 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier X-ray free-electron lasers (XFELs) show great promise for macromolecular structure determination from sub-micrometre-sized crystals, using the emerging method of serial femtosecond crystallography. The extreme brightness of the XFEL radiation can multiply ionize most, if not all, atoms in a protein, causing their scattering factors to change during the pulse, with a preferential `bleaching' of heavy atoms. This paper investigates the effects of electronic damage on experimental data collected from a Gd derivative of lysozyme microcrystals at different X-ray intensities, and the degree of ionization of Gd atoms is quantified from phased difference Fourier maps. A pattern sorting scheme is proposed to maximize the ionization contrast and the way in which the local electronic damage can be used for a new experimental phasing method is discussed. serial femtosecond crystallography high-intensity phasing radiation damage electronic damage X-ray free-electron lasers high XFEL doses Crystallography Sang-Kil Son verfasserin aut Thomas R. M. Barends verfasserin aut Thomas A. White verfasserin aut Anton Barty verfasserin aut Sabine Botha verfasserin aut Sébastien Boutet verfasserin aut Carl Caleman verfasserin aut R. Bruce Doak verfasserin aut Max H. Nanao verfasserin aut Karol Nass verfasserin aut Robert L. Shoeman verfasserin aut Nicusor Timneanu verfasserin aut Robin Santra verfasserin aut Ilme Schlichting verfasserin aut Henry N. Chapman verfasserin aut In IUCrJ International Union of Crystallography, 2014 2(2015), 6, Seite 627-634 (DE-627)777782758 (DE-600)2754953-7 20522525 nnns volume:2 year:2015 number:6 pages:627-634 https://doi.org/10.1107/S2052252515014049 kostenfrei https://doaj.org/article/470b97134cd24a229aa61150852a3784 kostenfrei http://scripts.iucr.org/cgi-bin/paper?S2052252515014049 kostenfrei https://doaj.org/toc/2052-2525 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2015 6 627-634
spelling	10.1107/S2052252515014049 doi (DE-627)DOAJ075583232 (DE-599)DOAJ470b97134cd24a229aa61150852a3784 DE-627 ger DE-627 rakwb eng QD901-999 Lorenzo Galli verfasserin aut Towards phasing using high X-ray intensity 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier X-ray free-electron lasers (XFELs) show great promise for macromolecular structure determination from sub-micrometre-sized crystals, using the emerging method of serial femtosecond crystallography. The extreme brightness of the XFEL radiation can multiply ionize most, if not all, atoms in a protein, causing their scattering factors to change during the pulse, with a preferential `bleaching' of heavy atoms. This paper investigates the effects of electronic damage on experimental data collected from a Gd derivative of lysozyme microcrystals at different X-ray intensities, and the degree of ionization of Gd atoms is quantified from phased difference Fourier maps. A pattern sorting scheme is proposed to maximize the ionization contrast and the way in which the local electronic damage can be used for a new experimental phasing method is discussed. serial femtosecond crystallography high-intensity phasing radiation damage electronic damage X-ray free-electron lasers high XFEL doses Crystallography Sang-Kil Son verfasserin aut Thomas R. M. Barends verfasserin aut Thomas A. White verfasserin aut Anton Barty verfasserin aut Sabine Botha verfasserin aut Sébastien Boutet verfasserin aut Carl Caleman verfasserin aut R. Bruce Doak verfasserin aut Max H. Nanao verfasserin aut Karol Nass verfasserin aut Robert L. Shoeman verfasserin aut Nicusor Timneanu verfasserin aut Robin Santra verfasserin aut Ilme Schlichting verfasserin aut Henry N. Chapman verfasserin aut In IUCrJ International Union of Crystallography, 2014 2(2015), 6, Seite 627-634 (DE-627)777782758 (DE-600)2754953-7 20522525 nnns volume:2 year:2015 number:6 pages:627-634 https://doi.org/10.1107/S2052252515014049 kostenfrei https://doaj.org/article/470b97134cd24a229aa61150852a3784 kostenfrei http://scripts.iucr.org/cgi-bin/paper?S2052252515014049 kostenfrei https://doaj.org/toc/2052-2525 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2015 6 627-634
allfields_unstemmed	10.1107/S2052252515014049 doi (DE-627)DOAJ075583232 (DE-599)DOAJ470b97134cd24a229aa61150852a3784 DE-627 ger DE-627 rakwb eng QD901-999 Lorenzo Galli verfasserin aut Towards phasing using high X-ray intensity 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier X-ray free-electron lasers (XFELs) show great promise for macromolecular structure determination from sub-micrometre-sized crystals, using the emerging method of serial femtosecond crystallography. The extreme brightness of the XFEL radiation can multiply ionize most, if not all, atoms in a protein, causing their scattering factors to change during the pulse, with a preferential `bleaching' of heavy atoms. This paper investigates the effects of electronic damage on experimental data collected from a Gd derivative of lysozyme microcrystals at different X-ray intensities, and the degree of ionization of Gd atoms is quantified from phased difference Fourier maps. A pattern sorting scheme is proposed to maximize the ionization contrast and the way in which the local electronic damage can be used for a new experimental phasing method is discussed. serial femtosecond crystallography high-intensity phasing radiation damage electronic damage X-ray free-electron lasers high XFEL doses Crystallography Sang-Kil Son verfasserin aut Thomas R. M. Barends verfasserin aut Thomas A. White verfasserin aut Anton Barty verfasserin aut Sabine Botha verfasserin aut Sébastien Boutet verfasserin aut Carl Caleman verfasserin aut R. Bruce Doak verfasserin aut Max H. Nanao verfasserin aut Karol Nass verfasserin aut Robert L. Shoeman verfasserin aut Nicusor Timneanu verfasserin aut Robin Santra verfasserin aut Ilme Schlichting verfasserin aut Henry N. Chapman verfasserin aut In IUCrJ International Union of Crystallography, 2014 2(2015), 6, Seite 627-634 (DE-627)777782758 (DE-600)2754953-7 20522525 nnns volume:2 year:2015 number:6 pages:627-634 https://doi.org/10.1107/S2052252515014049 kostenfrei https://doaj.org/article/470b97134cd24a229aa61150852a3784 kostenfrei http://scripts.iucr.org/cgi-bin/paper?S2052252515014049 kostenfrei https://doaj.org/toc/2052-2525 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2015 6 627-634
allfieldsGer	10.1107/S2052252515014049 doi (DE-627)DOAJ075583232 (DE-599)DOAJ470b97134cd24a229aa61150852a3784 DE-627 ger DE-627 rakwb eng QD901-999 Lorenzo Galli verfasserin aut Towards phasing using high X-ray intensity 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier X-ray free-electron lasers (XFELs) show great promise for macromolecular structure determination from sub-micrometre-sized crystals, using the emerging method of serial femtosecond crystallography. The extreme brightness of the XFEL radiation can multiply ionize most, if not all, atoms in a protein, causing their scattering factors to change during the pulse, with a preferential `bleaching' of heavy atoms. This paper investigates the effects of electronic damage on experimental data collected from a Gd derivative of lysozyme microcrystals at different X-ray intensities, and the degree of ionization of Gd atoms is quantified from phased difference Fourier maps. A pattern sorting scheme is proposed to maximize the ionization contrast and the way in which the local electronic damage can be used for a new experimental phasing method is discussed. serial femtosecond crystallography high-intensity phasing radiation damage electronic damage X-ray free-electron lasers high XFEL doses Crystallography Sang-Kil Son verfasserin aut Thomas R. M. Barends verfasserin aut Thomas A. White verfasserin aut Anton Barty verfasserin aut Sabine Botha verfasserin aut Sébastien Boutet verfasserin aut Carl Caleman verfasserin aut R. Bruce Doak verfasserin aut Max H. Nanao verfasserin aut Karol Nass verfasserin aut Robert L. Shoeman verfasserin aut Nicusor Timneanu verfasserin aut Robin Santra verfasserin aut Ilme Schlichting verfasserin aut Henry N. Chapman verfasserin aut In IUCrJ International Union of Crystallography, 2014 2(2015), 6, Seite 627-634 (DE-627)777782758 (DE-600)2754953-7 20522525 nnns volume:2 year:2015 number:6 pages:627-634 https://doi.org/10.1107/S2052252515014049 kostenfrei https://doaj.org/article/470b97134cd24a229aa61150852a3784 kostenfrei http://scripts.iucr.org/cgi-bin/paper?S2052252515014049 kostenfrei https://doaj.org/toc/2052-2525 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2015 6 627-634
allfieldsSound	10.1107/S2052252515014049 doi (DE-627)DOAJ075583232 (DE-599)DOAJ470b97134cd24a229aa61150852a3784 DE-627 ger DE-627 rakwb eng QD901-999 Lorenzo Galli verfasserin aut Towards phasing using high X-ray intensity 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier X-ray free-electron lasers (XFELs) show great promise for macromolecular structure determination from sub-micrometre-sized crystals, using the emerging method of serial femtosecond crystallography. The extreme brightness of the XFEL radiation can multiply ionize most, if not all, atoms in a protein, causing their scattering factors to change during the pulse, with a preferential `bleaching' of heavy atoms. This paper investigates the effects of electronic damage on experimental data collected from a Gd derivative of lysozyme microcrystals at different X-ray intensities, and the degree of ionization of Gd atoms is quantified from phased difference Fourier maps. A pattern sorting scheme is proposed to maximize the ionization contrast and the way in which the local electronic damage can be used for a new experimental phasing method is discussed. serial femtosecond crystallography high-intensity phasing radiation damage electronic damage X-ray free-electron lasers high XFEL doses Crystallography Sang-Kil Son verfasserin aut Thomas R. M. Barends verfasserin aut Thomas A. White verfasserin aut Anton Barty verfasserin aut Sabine Botha verfasserin aut Sébastien Boutet verfasserin aut Carl Caleman verfasserin aut R. Bruce Doak verfasserin aut Max H. Nanao verfasserin aut Karol Nass verfasserin aut Robert L. Shoeman verfasserin aut Nicusor Timneanu verfasserin aut Robin Santra verfasserin aut Ilme Schlichting verfasserin aut Henry N. Chapman verfasserin aut In IUCrJ International Union of Crystallography, 2014 2(2015), 6, Seite 627-634 (DE-627)777782758 (DE-600)2754953-7 20522525 nnns volume:2 year:2015 number:6 pages:627-634 https://doi.org/10.1107/S2052252515014049 kostenfrei https://doaj.org/article/470b97134cd24a229aa61150852a3784 kostenfrei http://scripts.iucr.org/cgi-bin/paper?S2052252515014049 kostenfrei https://doaj.org/toc/2052-2525 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2015 6 627-634
language	English
source	In IUCrJ 2(2015), 6, Seite 627-634 volume:2 year:2015 number:6 pages:627-634
sourceStr	In IUCrJ 2(2015), 6, Seite 627-634 volume:2 year:2015 number:6 pages:627-634
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	serial femtosecond crystallography high-intensity phasing radiation damage electronic damage X-ray free-electron lasers high XFEL doses Crystallography
isfreeaccess_bool	true
container_title	IUCrJ
authorswithroles_txt_mv	Lorenzo Galli @@aut@@ Sang-Kil Son @@aut@@ Thomas R. M. Barends @@aut@@ Thomas A. White @@aut@@ Anton Barty @@aut@@ Sabine Botha @@aut@@ Sébastien Boutet @@aut@@ Carl Caleman @@aut@@ R. Bruce Doak @@aut@@ Max H. Nanao @@aut@@ Karol Nass @@aut@@ Robert L. Shoeman @@aut@@ Nicusor Timneanu @@aut@@ Robin Santra @@aut@@ Ilme Schlichting @@aut@@ Henry N. Chapman @@aut@@
publishDateDaySort_date	2015-01-01T00:00:00Z
hierarchy_top_id	777782758
id	DOAJ075583232
language_de	englisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ075583232</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230309134713.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230228s2015 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1107/S2052252515014049</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ075583232</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ470b97134cd24a229aa61150852a3784</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QD901-999</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Lorenzo Galli</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Towards phasing using high X-ray intensity</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2015</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">X-ray free-electron lasers (XFELs) show great promise for macromolecular structure determination from sub-micrometre-sized crystals, using the emerging method of serial femtosecond crystallography. The extreme brightness of the XFEL radiation can multiply ionize most, if not all, atoms in a protein, causing their scattering factors to change during the pulse, with a preferential `bleaching' of heavy atoms. This paper investigates the effects of electronic damage on experimental data collected from a Gd derivative of lysozyme microcrystals at different X-ray intensities, and the degree of ionization of Gd atoms is quantified from phased difference Fourier maps. A pattern sorting scheme is proposed to maximize the ionization contrast and the way in which the local electronic damage can be used for a new experimental phasing method is discussed.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">serial femtosecond crystallography</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">high-intensity phasing</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">radiation damage</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">electronic damage</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">X-ray free-electron lasers</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">high XFEL doses</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Crystallography</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Sang-Kil Son</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Thomas R. M. Barends</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Thomas A. White</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Anton Barty</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Sabine Botha</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Sébastien Boutet</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Carl Caleman</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">R. Bruce Doak</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Max H. Nanao</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Karol Nass</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Robert L. Shoeman</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Nicusor Timneanu</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Robin Santra</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Ilme Schlichting</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Henry N. Chapman</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">IUCrJ</subfield><subfield code="d">International Union of Crystallography, 2014</subfield><subfield code="g">2(2015), 6, Seite 627-634</subfield><subfield code="w">(DE-627)777782758</subfield><subfield code="w">(DE-600)2754953-7</subfield><subfield code="x">20522525</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:2</subfield><subfield code="g">year:2015</subfield><subfield code="g">number:6</subfield><subfield code="g">pages:627-634</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1107/S2052252515014049</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/470b97134cd24a229aa61150852a3784</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://scripts.iucr.org/cgi-bin/paper?S2052252515014049</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/2052-2525</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_11</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_370</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2003</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4335</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">2</subfield><subfield code="j">2015</subfield><subfield code="e">6</subfield><subfield code="h">627-634</subfield></datafield></record></collection>
callnumber-first	Q - Science
author	Lorenzo Galli
spellingShingle	Lorenzo Galli misc QD901-999 misc serial femtosecond crystallography misc high-intensity phasing misc radiation damage misc electronic damage misc X-ray free-electron lasers misc high XFEL doses misc Crystallography Towards phasing using high X-ray intensity
authorStr	Lorenzo Galli
ppnlink_with_tag_str_mv	@@773@@(DE-627)777782758
format	electronic Article
delete_txt_mv	keep
author_role	aut aut aut aut aut aut aut aut aut aut aut aut aut aut aut aut
collection	DOAJ
remote_str	true
callnumber-label	QD901-999
illustrated	Not Illustrated
issn	20522525
topic_title	QD901-999 Towards phasing using high X-ray intensity serial femtosecond crystallography high-intensity phasing radiation damage electronic damage X-ray free-electron lasers high XFEL doses
topic	misc QD901-999 misc serial femtosecond crystallography misc high-intensity phasing misc radiation damage misc electronic damage misc X-ray free-electron lasers misc high XFEL doses misc Crystallography
topic_unstemmed	misc QD901-999 misc serial femtosecond crystallography misc high-intensity phasing misc radiation damage misc electronic damage misc X-ray free-electron lasers misc high XFEL doses misc Crystallography
topic_browse	misc QD901-999 misc serial femtosecond crystallography misc high-intensity phasing misc radiation damage misc electronic damage misc X-ray free-electron lasers misc high XFEL doses misc Crystallography
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
format_main_str_mv	Text Zeitschrift/Artikel
carriertype_str_mv	cr
hierarchy_parent_title	IUCrJ
hierarchy_parent_id	777782758
hierarchy_top_title	IUCrJ
isfreeaccess_txt	true
familylinks_str_mv	(DE-627)777782758 (DE-600)2754953-7
title	Towards phasing using high X-ray intensity
ctrlnum	(DE-627)DOAJ075583232 (DE-599)DOAJ470b97134cd24a229aa61150852a3784
title_full	Towards phasing using high X-ray intensity
author_sort	Lorenzo Galli
journal	IUCrJ
journalStr	IUCrJ
callnumber-first-code	Q
lang_code	eng
isOA_bool	true
recordtype	marc
publishDateSort	2015
contenttype_str_mv	txt
container_start_page	627
author_browse	Lorenzo Galli Sang-Kil Son Thomas R. M. Barends Thomas A. White Anton Barty Sabine Botha Sébastien Boutet Carl Caleman R. Bruce Doak Max H. Nanao Karol Nass Robert L. Shoeman Nicusor Timneanu Robin Santra Ilme Schlichting Henry N. Chapman
container_volume	2
class	QD901-999
format_se	Elektronische Aufsätze
author-letter	Lorenzo Galli
doi_str_mv	10.1107/S2052252515014049
author2-role	verfasserin
title_sort	towards phasing using high x-ray intensity
callnumber	QD901-999
title_auth	Towards phasing using high X-ray intensity
abstract	X-ray free-electron lasers (XFELs) show great promise for macromolecular structure determination from sub-micrometre-sized crystals, using the emerging method of serial femtosecond crystallography. The extreme brightness of the XFEL radiation can multiply ionize most, if not all, atoms in a protein, causing their scattering factors to change during the pulse, with a preferential `bleaching' of heavy atoms. This paper investigates the effects of electronic damage on experimental data collected from a Gd derivative of lysozyme microcrystals at different X-ray intensities, and the degree of ionization of Gd atoms is quantified from phased difference Fourier maps. A pattern sorting scheme is proposed to maximize the ionization contrast and the way in which the local electronic damage can be used for a new experimental phasing method is discussed.
abstractGer	X-ray free-electron lasers (XFELs) show great promise for macromolecular structure determination from sub-micrometre-sized crystals, using the emerging method of serial femtosecond crystallography. The extreme brightness of the XFEL radiation can multiply ionize most, if not all, atoms in a protein, causing their scattering factors to change during the pulse, with a preferential `bleaching' of heavy atoms. This paper investigates the effects of electronic damage on experimental data collected from a Gd derivative of lysozyme microcrystals at different X-ray intensities, and the degree of ionization of Gd atoms is quantified from phased difference Fourier maps. A pattern sorting scheme is proposed to maximize the ionization contrast and the way in which the local electronic damage can be used for a new experimental phasing method is discussed.
abstract_unstemmed	X-ray free-electron lasers (XFELs) show great promise for macromolecular structure determination from sub-micrometre-sized crystals, using the emerging method of serial femtosecond crystallography. The extreme brightness of the XFEL radiation can multiply ionize most, if not all, atoms in a protein, causing their scattering factors to change during the pulse, with a preferential `bleaching' of heavy atoms. This paper investigates the effects of electronic damage on experimental data collected from a Gd derivative of lysozyme microcrystals at different X-ray intensities, and the degree of ionization of Gd atoms is quantified from phased difference Fourier maps. A pattern sorting scheme is proposed to maximize the ionization contrast and the way in which the local electronic damage can be used for a new experimental phasing method is discussed.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
container_issue	6
title_short	Towards phasing using high X-ray intensity
url	https://doi.org/10.1107/S2052252515014049 https://doaj.org/article/470b97134cd24a229aa61150852a3784 http://scripts.iucr.org/cgi-bin/paper?S2052252515014049 https://doaj.org/toc/2052-2525
remote_bool	true
author2	Sang-Kil Son Thomas R. M. Barends Thomas A. White Anton Barty Sabine Botha Sébastien Boutet Carl Caleman R. Bruce Doak Max H. Nanao Karol Nass Robert L. Shoeman Nicusor Timneanu Robin Santra Ilme Schlichting Henry N. Chapman
author2Str	Sang-Kil Son Thomas R. M. Barends Thomas A. White Anton Barty Sabine Botha Sébastien Boutet Carl Caleman R. Bruce Doak Max H. Nanao Karol Nass Robert L. Shoeman Nicusor Timneanu Robin Santra Ilme Schlichting Henry N. Chapman
ppnlink	777782758
callnumber-subject	QD - Chemistry
mediatype_str_mv	c
isOA_txt	true
hochschulschrift_bool	false
doi_str	10.1107/S2052252515014049
callnumber-a	QD901-999
up_date	2024-07-03T15:43:38.201Z
_version_	1803573178614677504
fullrecord_marcxml	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ075583232</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230309134713.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230228s2015 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1107/S2052252515014049</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ075583232</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ470b97134cd24a229aa61150852a3784</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QD901-999</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Lorenzo Galli</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Towards phasing using high X-ray intensity</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2015</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">X-ray free-electron lasers (XFELs) show great promise for macromolecular structure determination from sub-micrometre-sized crystals, using the emerging method of serial femtosecond crystallography. The extreme brightness of the XFEL radiation can multiply ionize most, if not all, atoms in a protein, causing their scattering factors to change during the pulse, with a preferential `bleaching' of heavy atoms. This paper investigates the effects of electronic damage on experimental data collected from a Gd derivative of lysozyme microcrystals at different X-ray intensities, and the degree of ionization of Gd atoms is quantified from phased difference Fourier maps. A pattern sorting scheme is proposed to maximize the ionization contrast and the way in which the local electronic damage can be used for a new experimental phasing method is discussed.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">serial femtosecond crystallography</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">high-intensity phasing</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">radiation damage</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">electronic damage</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">X-ray free-electron lasers</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">high XFEL doses</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Crystallography</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Sang-Kil Son</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Thomas R. M. Barends</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Thomas A. White</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Anton Barty</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Sabine Botha</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Sébastien Boutet</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Carl Caleman</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">R. Bruce Doak</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Max H. Nanao</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Karol Nass</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Robert L. Shoeman</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Nicusor Timneanu</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Robin Santra</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Ilme Schlichting</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Henry N. Chapman</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">IUCrJ</subfield><subfield code="d">International Union of Crystallography, 2014</subfield><subfield code="g">2(2015), 6, Seite 627-634</subfield><subfield code="w">(DE-627)777782758</subfield><subfield code="w">(DE-600)2754953-7</subfield><subfield code="x">20522525</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:2</subfield><subfield code="g">year:2015</subfield><subfield code="g">number:6</subfield><subfield code="g">pages:627-634</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1107/S2052252515014049</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/470b97134cd24a229aa61150852a3784</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://scripts.iucr.org/cgi-bin/paper?S2052252515014049</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/2052-2525</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_11</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_370</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2003</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4335</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">2</subfield><subfield code="j">2015</subfield><subfield code="e">6</subfield><subfield code="h">627-634</subfield></datafield></record></collection>
score	7.3994513

Nicht das Richtige dabei?

Schreiben Sie uns!

Towards phasing using high X-ray intensity

Nicht das Richtige dabei?

Zugang & Verfügbarkeit

Vorhandene Bände

Nicht das Richtige dabei?