Serological identification of house dust mite allergens in dogs with atopic dermatitis

House dust mite antigens have been used for decades to diagnose allergic diseases in humans and animals. The objective of this study was to identify allergens in commercial Dermatophagoides farinae and Blomia tropicalis extracts by immunoblotting using sera from allergic dogs and anti-dog IgE conjug...
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Autor*in:	Victor E.S. Cunha [verfasserIn] Maria Helena Silva [verfasserIn] João Luiz H. Faccini [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch ; Portugiesisch

Erschienen:	2012

Schlagwörter:	Dermatite atópica alérgenos cães Dermatophagoides farinae Blomia tropicalis

Übergeordnetes Werk:	In: Pesquisa Veterinária Brasileira - Colégio Brasileiro de Patologia Animal (CBPA), 2004, 32(2012), 9, Seite 917-921
Übergeordnetes Werk:	volume:32 ; year:2012 ; number:9 ; pages:917-921

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1590/S0100-736X2012000900016

Katalog-ID:	DOAJ075635232

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language	English Portuguese
source	In Pesquisa Veterinária Brasileira 32(2012), 9, Seite 917-921 volume:32 year:2012 number:9 pages:917-921
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topic_facet	Dermatite atópica alérgenos cães Dermatophagoides farinae Blomia tropicalis Veterinary medicine
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container_title	Pesquisa Veterinária Brasileira
authorswithroles_txt_mv	Victor E.S. Cunha @@aut@@ Maria Helena Silva @@aut@@ João Luiz H. Faccini @@aut@@
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callnumber-first	S - Agriculture
author	Victor E.S. Cunha
spellingShingle	Victor E.S. Cunha misc SF600-1100 misc Dermatite atópica misc alérgenos misc cães misc Dermatophagoides farinae misc Blomia tropicalis misc Veterinary medicine Serological identification of house dust mite allergens in dogs with atopic dermatitis
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topic_title	SF600-1100 Serological identification of house dust mite allergens in dogs with atopic dermatitis Dermatite atópica alérgenos cães Dermatophagoides farinae Blomia tropicalis
topic	misc SF600-1100 misc Dermatite atópica misc alérgenos misc cães misc Dermatophagoides farinae misc Blomia tropicalis misc Veterinary medicine
topic_unstemmed	misc SF600-1100 misc Dermatite atópica misc alérgenos misc cães misc Dermatophagoides farinae misc Blomia tropicalis misc Veterinary medicine
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title	Serological identification of house dust mite allergens in dogs with atopic dermatitis
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title_full	Serological identification of house dust mite allergens in dogs with atopic dermatitis
author_sort	Victor E.S. Cunha
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title_sort	serological identification of house dust mite allergens in dogs with atopic dermatitis
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title_auth	Serological identification of house dust mite allergens in dogs with atopic dermatitis
abstract	House dust mite antigens have been used for decades to diagnose allergic diseases in humans and animals. The objective of this study was to identify allergens in commercial Dermatophagoides farinae and Blomia tropicalis extracts by immunoblotting using sera from allergic dogs and anti-dog IgE conjugate. The analysis of antigens present in the D. farinae extract (FDA Allergenic) using sera from 10 dogs allergic to D. farinae showed that eight sera recognized a band of approximately 102 kDa, eight recognized two bands of 52 to 76 kDa, five recognized one band of approximately 76 kDa, four recognized one band of 31 to 38 kDa, and two recognized one band of 12 to 17 kDa. Immunoblot assays of the B. tropicalis extract (FDA Allergenic) using sera from 10 animals allergic to B. tropicalis showed that five sera recognized two bands of 52 to 76 kDa. These results demonstrate the importance of the two house dust mite species for the pathogenesis of canine atopic dermatitis in Brazil. In addition, the results indicate which allergens should be present in allergenic extracts used for diagnosis and allergen-specific immunotherapy.
abstractGer	House dust mite antigens have been used for decades to diagnose allergic diseases in humans and animals. The objective of this study was to identify allergens in commercial Dermatophagoides farinae and Blomia tropicalis extracts by immunoblotting using sera from allergic dogs and anti-dog IgE conjugate. The analysis of antigens present in the D. farinae extract (FDA Allergenic) using sera from 10 dogs allergic to D. farinae showed that eight sera recognized a band of approximately 102 kDa, eight recognized two bands of 52 to 76 kDa, five recognized one band of approximately 76 kDa, four recognized one band of 31 to 38 kDa, and two recognized one band of 12 to 17 kDa. Immunoblot assays of the B. tropicalis extract (FDA Allergenic) using sera from 10 animals allergic to B. tropicalis showed that five sera recognized two bands of 52 to 76 kDa. These results demonstrate the importance of the two house dust mite species for the pathogenesis of canine atopic dermatitis in Brazil. In addition, the results indicate which allergens should be present in allergenic extracts used for diagnosis and allergen-specific immunotherapy.
abstract_unstemmed	House dust mite antigens have been used for decades to diagnose allergic diseases in humans and animals. The objective of this study was to identify allergens in commercial Dermatophagoides farinae and Blomia tropicalis extracts by immunoblotting using sera from allergic dogs and anti-dog IgE conjugate. The analysis of antigens present in the D. farinae extract (FDA Allergenic) using sera from 10 dogs allergic to D. farinae showed that eight sera recognized a band of approximately 102 kDa, eight recognized two bands of 52 to 76 kDa, five recognized one band of approximately 76 kDa, four recognized one band of 31 to 38 kDa, and two recognized one band of 12 to 17 kDa. Immunoblot assays of the B. tropicalis extract (FDA Allergenic) using sera from 10 animals allergic to B. tropicalis showed that five sera recognized two bands of 52 to 76 kDa. These results demonstrate the importance of the two house dust mite species for the pathogenesis of canine atopic dermatitis in Brazil. In addition, the results indicate which allergens should be present in allergenic extracts used for diagnosis and allergen-specific immunotherapy.
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title_short	Serological identification of house dust mite allergens in dogs with atopic dermatitis
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Serological identification of house dust mite allergens in dogs with atopic dermatitis

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