Small RNA Profiling in Mycobacterium Provides Insights Into Stress Adaptability

Mycobacteria encounter a number of environmental changes during infection and respond using different mechanisms. Small RNA (sRNA) is a post-transcriptionally regulatory system for gene functions and has been investigated in many other bacteria. This study used Mycobacterium tuberculosis and Mycobac...
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Autor*in:	Yingyu Chen [verfasserIn] Wenjun Zhai [verfasserIn] Kailun Zhang [verfasserIn] Han Liu [verfasserIn] Tingting Zhu [verfasserIn] Li Su [verfasserIn] Luiz Bermudez [verfasserIn] Huanchun Chen [verfasserIn] Aizhen Guo [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	Mycobacterium bovis Mycobacterium bovis BCG sRNA stress mycobacteria

Übergeordnetes Werk:	In: Frontiers in Microbiology - Frontiers Media S.A., 2011, 12(2021)
Übergeordnetes Werk:	volume:12 ; year:2021

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fmicb.2021.752537

Katalog-ID:	DOAJ075651246

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spelling	10.3389/fmicb.2021.752537 doi (DE-627)DOAJ075651246 (DE-599)DOAJ8eef4f7fd8074eddbb5b486d19aa34f4 DE-627 ger DE-627 rakwb eng QR1-502 Yingyu Chen verfasserin aut Small RNA Profiling in Mycobacterium Provides Insights Into Stress Adaptability 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Mycobacteria encounter a number of environmental changes during infection and respond using different mechanisms. Small RNA (sRNA) is a post-transcriptionally regulatory system for gene functions and has been investigated in many other bacteria. This study used Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-Guérin (BCG) infection models and sequenced whole bacterial RNAs before and after host cell infection. A comparison of differentially expressed sRNAs using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) and target prediction was carried out. Six pathogenically relevant stress conditions, growth rate, and morphology were used to screen and identify sRNAs. From these data, a subset of sRNAs was differentially expressed in multiple infection groups and stress conditions. Many were found associated with lipid metabolism. Among them, ncBCG427 was significantly downregulated when BCG entered into macrophages and was associated with increased biofilm formation. The reduction of virulence possibility depends on regulating lipid metabolism. Mycobacterium bovis Mycobacterium bovis BCG sRNA stress mycobacteria Microbiology Yingyu Chen verfasserin aut Wenjun Zhai verfasserin aut Wenjun Zhai verfasserin aut Kailun Zhang verfasserin aut Kailun Zhang verfasserin aut Han Liu verfasserin aut Han Liu verfasserin aut Tingting Zhu verfasserin aut Tingting Zhu verfasserin aut Li Su verfasserin aut Li Su verfasserin aut Luiz Bermudez verfasserin aut Huanchun Chen verfasserin aut Huanchun Chen verfasserin aut Aizhen Guo verfasserin aut Aizhen Guo verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 12(2021) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:12 year:2021 https://doi.org/10.3389/fmicb.2021.752537 kostenfrei https://doaj.org/article/8eef4f7fd8074eddbb5b486d19aa34f4 kostenfrei https://www.frontiersin.org/articles/10.3389/fmicb.2021.752537/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021
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allfieldsSound	10.3389/fmicb.2021.752537 doi (DE-627)DOAJ075651246 (DE-599)DOAJ8eef4f7fd8074eddbb5b486d19aa34f4 DE-627 ger DE-627 rakwb eng QR1-502 Yingyu Chen verfasserin aut Small RNA Profiling in Mycobacterium Provides Insights Into Stress Adaptability 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Mycobacteria encounter a number of environmental changes during infection and respond using different mechanisms. Small RNA (sRNA) is a post-transcriptionally regulatory system for gene functions and has been investigated in many other bacteria. This study used Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-Guérin (BCG) infection models and sequenced whole bacterial RNAs before and after host cell infection. A comparison of differentially expressed sRNAs using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) and target prediction was carried out. Six pathogenically relevant stress conditions, growth rate, and morphology were used to screen and identify sRNAs. From these data, a subset of sRNAs was differentially expressed in multiple infection groups and stress conditions. Many were found associated with lipid metabolism. Among them, ncBCG427 was significantly downregulated when BCG entered into macrophages and was associated with increased biofilm formation. The reduction of virulence possibility depends on regulating lipid metabolism. Mycobacterium bovis Mycobacterium bovis BCG sRNA stress mycobacteria Microbiology Yingyu Chen verfasserin aut Wenjun Zhai verfasserin aut Wenjun Zhai verfasserin aut Kailun Zhang verfasserin aut Kailun Zhang verfasserin aut Han Liu verfasserin aut Han Liu verfasserin aut Tingting Zhu verfasserin aut Tingting Zhu verfasserin aut Li Su verfasserin aut Li Su verfasserin aut Luiz Bermudez verfasserin aut Huanchun Chen verfasserin aut Huanchun Chen verfasserin aut Aizhen Guo verfasserin aut Aizhen Guo verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 12(2021) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:12 year:2021 https://doi.org/10.3389/fmicb.2021.752537 kostenfrei https://doaj.org/article/8eef4f7fd8074eddbb5b486d19aa34f4 kostenfrei https://www.frontiersin.org/articles/10.3389/fmicb.2021.752537/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021
language	English
source	In Frontiers in Microbiology 12(2021) volume:12 year:2021
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topic_facet	Mycobacterium bovis Mycobacterium bovis BCG sRNA stress mycobacteria Microbiology
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container_title	Frontiers in Microbiology
authorswithroles_txt_mv	Yingyu Chen @@aut@@ Wenjun Zhai @@aut@@ Kailun Zhang @@aut@@ Han Liu @@aut@@ Tingting Zhu @@aut@@ Li Su @@aut@@ Luiz Bermudez @@aut@@ Huanchun Chen @@aut@@ Aizhen Guo @@aut@@
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callnumber-first	Q - Science
author	Yingyu Chen
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topic_title	QR1-502 Small RNA Profiling in Mycobacterium Provides Insights Into Stress Adaptability Mycobacterium bovis Mycobacterium bovis BCG sRNA stress mycobacteria
topic	misc QR1-502 misc Mycobacterium bovis misc Mycobacterium bovis BCG misc sRNA misc stress misc mycobacteria misc Microbiology
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title	Small RNA Profiling in Mycobacterium Provides Insights Into Stress Adaptability
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title_full	Small RNA Profiling in Mycobacterium Provides Insights Into Stress Adaptability
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title_sort	small rna profiling in mycobacterium provides insights into stress adaptability
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title_auth	Small RNA Profiling in Mycobacterium Provides Insights Into Stress Adaptability
abstract	Mycobacteria encounter a number of environmental changes during infection and respond using different mechanisms. Small RNA (sRNA) is a post-transcriptionally regulatory system for gene functions and has been investigated in many other bacteria. This study used Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-Guérin (BCG) infection models and sequenced whole bacterial RNAs before and after host cell infection. A comparison of differentially expressed sRNAs using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) and target prediction was carried out. Six pathogenically relevant stress conditions, growth rate, and morphology were used to screen and identify sRNAs. From these data, a subset of sRNAs was differentially expressed in multiple infection groups and stress conditions. Many were found associated with lipid metabolism. Among them, ncBCG427 was significantly downregulated when BCG entered into macrophages and was associated with increased biofilm formation. The reduction of virulence possibility depends on regulating lipid metabolism.
abstractGer	Mycobacteria encounter a number of environmental changes during infection and respond using different mechanisms. Small RNA (sRNA) is a post-transcriptionally regulatory system for gene functions and has been investigated in many other bacteria. This study used Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-Guérin (BCG) infection models and sequenced whole bacterial RNAs before and after host cell infection. A comparison of differentially expressed sRNAs using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) and target prediction was carried out. Six pathogenically relevant stress conditions, growth rate, and morphology were used to screen and identify sRNAs. From these data, a subset of sRNAs was differentially expressed in multiple infection groups and stress conditions. Many were found associated with lipid metabolism. Among them, ncBCG427 was significantly downregulated when BCG entered into macrophages and was associated with increased biofilm formation. The reduction of virulence possibility depends on regulating lipid metabolism.
abstract_unstemmed	Mycobacteria encounter a number of environmental changes during infection and respond using different mechanisms. Small RNA (sRNA) is a post-transcriptionally regulatory system for gene functions and has been investigated in many other bacteria. This study used Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-Guérin (BCG) infection models and sequenced whole bacterial RNAs before and after host cell infection. A comparison of differentially expressed sRNAs using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) and target prediction was carried out. Six pathogenically relevant stress conditions, growth rate, and morphology were used to screen and identify sRNAs. From these data, a subset of sRNAs was differentially expressed in multiple infection groups and stress conditions. Many were found associated with lipid metabolism. Among them, ncBCG427 was significantly downregulated when BCG entered into macrophages and was associated with increased biofilm formation. The reduction of virulence possibility depends on regulating lipid metabolism.
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title_short	Small RNA Profiling in Mycobacterium Provides Insights Into Stress Adaptability
url	https://doi.org/10.3389/fmicb.2021.752537 https://doaj.org/article/8eef4f7fd8074eddbb5b486d19aa34f4 https://www.frontiersin.org/articles/10.3389/fmicb.2021.752537/full https://doaj.org/toc/1664-302X
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up_date	2024-07-03T16:05:32.835Z
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