Applications of capacitation status for litter size enhancement in various pig breeds

Objective Several studies have reported the development of new molecular methods for the prognosis and diagnosis of male fertility based on biomarkers aimed at overcoming the limitations of conventional male fertility analysis tools. However, further studies are needed for the field application of these methods. Therefore, alternative methods based on existing semen analysis methods are required to improve production efficiency in the animal industry. Methods we examined the possibility of improving litter size in various pig breeds using combined Hoechst 33258/chlortetracycline fluorescence (H33258/CTC) staining. The correlation between field fertility and capacitation status by combined H33258/CTC staining in different ejaculates spermatozoa (n = 3) from an individual boar (20 Landrace, 20 Yorkshire, and 20 Duroc) was evaluated as well as overall accuracy. Results The acrosome reacted (AR) pattern after capacitation (%) was positively correlated with the litter size of Landrace, Yorkshire, and Duroc pigs and the overall accuracy was 75%, 75%, and 70% in Landrace, Yorkshire, and Duroc pigs, respectively. The difference (Δ) in AR pattern before and after capacitation was positively correlated with the litter size of Landrace, Yorkshire, and Duroc pigs and the overall accuracy was 80%, 65%, and 55% in Landrace, Yorkshire, and Duroc pigs, respectively. However, the difference (Δ) in capacitated (B) pattern before and after capacitation was negatively correlated with the litter size of Landrace pigs and the overall accuracy was 75%. Moreover, average litter size was significantly altered according to different combined H33258/CTC staining parameters. Conclusion These results show that combined H33258/CTC staining may be used to predict male fertility in various breeds. However, the selection of specific efficiency combined H33258/CTC staining parameters requires further consideration. Taken together, these findings suggest that combined H33258/CTC staining may constitute an alternative method for predicting male fertility until such time as fertility-related biomarkers are further validated. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Woo-Sung Kwon [verfasserIn] Dong-Ha Shin [verfasserIn] Do-Yeal Ryu [verfasserIn] Amena Khatun [verfasserIn] Md Saidur Rahman [verfasserIn] Myung-Geol Pang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Schlagwörter:	Litter Size Capacitation Status Boar Spermatozoa Breeds Fertility

Übergeordnetes Werk:	In: Asian-Australasian Journal of Animal Sciences - Asian-Australasian Association of Animal Production Societies, 2017, 31(2018), 6, Seite 842-850
Übergeordnetes Werk:	volume:31 ; year:2018 ; number:6 ; pages:842-850

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.5713/ajas.17.0760

Katalog-ID:	DOAJ075676133

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520			\|a Objective Several studies have reported the development of new molecular methods for the prognosis and diagnosis of male fertility based on biomarkers aimed at overcoming the limitations of conventional male fertility analysis tools. However, further studies are needed for the field application of these methods. Therefore, alternative methods based on existing semen analysis methods are required to improve production efficiency in the animal industry. Methods we examined the possibility of improving litter size in various pig breeds using combined Hoechst 33258/chlortetracycline fluorescence (H33258/CTC) staining. The correlation between field fertility and capacitation status by combined H33258/CTC staining in different ejaculates spermatozoa (n = 3) from an individual boar (20 Landrace, 20 Yorkshire, and 20 Duroc) was evaluated as well as overall accuracy. Results The acrosome reacted (AR) pattern after capacitation (%) was positively correlated with the litter size of Landrace, Yorkshire, and Duroc pigs and the overall accuracy was 75%, 75%, and 70% in Landrace, Yorkshire, and Duroc pigs, respectively. The difference (Δ) in AR pattern before and after capacitation was positively correlated with the litter size of Landrace, Yorkshire, and Duroc pigs and the overall accuracy was 80%, 65%, and 55% in Landrace, Yorkshire, and Duroc pigs, respectively. However, the difference (Δ) in capacitated (B) pattern before and after capacitation was negatively correlated with the litter size of Landrace pigs and the overall accuracy was 75%. Moreover, average litter size was significantly altered according to different combined H33258/CTC staining parameters. Conclusion These results show that combined H33258/CTC staining may be used to predict male fertility in various breeds. However, the selection of specific efficiency combined H33258/CTC staining parameters requires further consideration. Taken together, these findings suggest that combined H33258/CTC staining may constitute an alternative method for predicting male fertility until such time as fertility-related biomarkers are further validated.
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650		4	\|a Capacitation Status
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allfields	10.5713/ajas.17.0760 doi (DE-627)DOAJ075676133 (DE-599)DOAJ4f9053579627438286acffb5821a778c DE-627 ger DE-627 rakwb eng SF1-1100 QP501-801 Woo-Sung Kwon verfasserin aut Applications of capacitation status for litter size enhancement in various pig breeds 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective Several studies have reported the development of new molecular methods for the prognosis and diagnosis of male fertility based on biomarkers aimed at overcoming the limitations of conventional male fertility analysis tools. However, further studies are needed for the field application of these methods. Therefore, alternative methods based on existing semen analysis methods are required to improve production efficiency in the animal industry. Methods we examined the possibility of improving litter size in various pig breeds using combined Hoechst 33258/chlortetracycline fluorescence (H33258/CTC) staining. The correlation between field fertility and capacitation status by combined H33258/CTC staining in different ejaculates spermatozoa (n = 3) from an individual boar (20 Landrace, 20 Yorkshire, and 20 Duroc) was evaluated as well as overall accuracy. Results The acrosome reacted (AR) pattern after capacitation (%) was positively correlated with the litter size of Landrace, Yorkshire, and Duroc pigs and the overall accuracy was 75%, 75%, and 70% in Landrace, Yorkshire, and Duroc pigs, respectively. The difference (Δ) in AR pattern before and after capacitation was positively correlated with the litter size of Landrace, Yorkshire, and Duroc pigs and the overall accuracy was 80%, 65%, and 55% in Landrace, Yorkshire, and Duroc pigs, respectively. However, the difference (Δ) in capacitated (B) pattern before and after capacitation was negatively correlated with the litter size of Landrace pigs and the overall accuracy was 75%. Moreover, average litter size was significantly altered according to different combined H33258/CTC staining parameters. Conclusion These results show that combined H33258/CTC staining may be used to predict male fertility in various breeds. However, the selection of specific efficiency combined H33258/CTC staining parameters requires further consideration. Taken together, these findings suggest that combined H33258/CTC staining may constitute an alternative method for predicting male fertility until such time as fertility-related biomarkers are further validated. Litter Size Capacitation Status Boar Spermatozoa Breeds Fertility Animal culture Animal biochemistry Dong-Ha Shin verfasserin aut Do-Yeal Ryu verfasserin aut Amena Khatun verfasserin aut Md Saidur Rahman verfasserin aut Myung-Geol Pang verfasserin aut In Asian-Australasian Journal of Animal Sciences Asian-Australasian Association of Animal Production Societies, 2017 31(2018), 6, Seite 842-850 (DE-627)756825873 (DE-600)2727534-6 19765517 nnns volume:31 year:2018 number:6 pages:842-850 https://doi.org/10.5713/ajas.17.0760 kostenfrei https://doaj.org/article/4f9053579627438286acffb5821a778c kostenfrei http://www.ajas.info/upload/pdf/ajas-31-6-842.pdf kostenfrei https://doaj.org/toc/1011-2367 Journal toc kostenfrei https://doaj.org/toc/1976-5517 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 31 2018 6 842-850
spelling	10.5713/ajas.17.0760 doi (DE-627)DOAJ075676133 (DE-599)DOAJ4f9053579627438286acffb5821a778c DE-627 ger DE-627 rakwb eng SF1-1100 QP501-801 Woo-Sung Kwon verfasserin aut Applications of capacitation status for litter size enhancement in various pig breeds 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective Several studies have reported the development of new molecular methods for the prognosis and diagnosis of male fertility based on biomarkers aimed at overcoming the limitations of conventional male fertility analysis tools. However, further studies are needed for the field application of these methods. Therefore, alternative methods based on existing semen analysis methods are required to improve production efficiency in the animal industry. Methods we examined the possibility of improving litter size in various pig breeds using combined Hoechst 33258/chlortetracycline fluorescence (H33258/CTC) staining. The correlation between field fertility and capacitation status by combined H33258/CTC staining in different ejaculates spermatozoa (n = 3) from an individual boar (20 Landrace, 20 Yorkshire, and 20 Duroc) was evaluated as well as overall accuracy. Results The acrosome reacted (AR) pattern after capacitation (%) was positively correlated with the litter size of Landrace, Yorkshire, and Duroc pigs and the overall accuracy was 75%, 75%, and 70% in Landrace, Yorkshire, and Duroc pigs, respectively. The difference (Δ) in AR pattern before and after capacitation was positively correlated with the litter size of Landrace, Yorkshire, and Duroc pigs and the overall accuracy was 80%, 65%, and 55% in Landrace, Yorkshire, and Duroc pigs, respectively. However, the difference (Δ) in capacitated (B) pattern before and after capacitation was negatively correlated with the litter size of Landrace pigs and the overall accuracy was 75%. Moreover, average litter size was significantly altered according to different combined H33258/CTC staining parameters. Conclusion These results show that combined H33258/CTC staining may be used to predict male fertility in various breeds. However, the selection of specific efficiency combined H33258/CTC staining parameters requires further consideration. Taken together, these findings suggest that combined H33258/CTC staining may constitute an alternative method for predicting male fertility until such time as fertility-related biomarkers are further validated. Litter Size Capacitation Status Boar Spermatozoa Breeds Fertility Animal culture Animal biochemistry Dong-Ha Shin verfasserin aut Do-Yeal Ryu verfasserin aut Amena Khatun verfasserin aut Md Saidur Rahman verfasserin aut Myung-Geol Pang verfasserin aut In Asian-Australasian Journal of Animal Sciences Asian-Australasian Association of Animal Production Societies, 2017 31(2018), 6, Seite 842-850 (DE-627)756825873 (DE-600)2727534-6 19765517 nnns volume:31 year:2018 number:6 pages:842-850 https://doi.org/10.5713/ajas.17.0760 kostenfrei https://doaj.org/article/4f9053579627438286acffb5821a778c kostenfrei http://www.ajas.info/upload/pdf/ajas-31-6-842.pdf kostenfrei https://doaj.org/toc/1011-2367 Journal toc kostenfrei https://doaj.org/toc/1976-5517 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 31 2018 6 842-850
allfields_unstemmed	10.5713/ajas.17.0760 doi (DE-627)DOAJ075676133 (DE-599)DOAJ4f9053579627438286acffb5821a778c DE-627 ger DE-627 rakwb eng SF1-1100 QP501-801 Woo-Sung Kwon verfasserin aut Applications of capacitation status for litter size enhancement in various pig breeds 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective Several studies have reported the development of new molecular methods for the prognosis and diagnosis of male fertility based on biomarkers aimed at overcoming the limitations of conventional male fertility analysis tools. However, further studies are needed for the field application of these methods. Therefore, alternative methods based on existing semen analysis methods are required to improve production efficiency in the animal industry. Methods we examined the possibility of improving litter size in various pig breeds using combined Hoechst 33258/chlortetracycline fluorescence (H33258/CTC) staining. The correlation between field fertility and capacitation status by combined H33258/CTC staining in different ejaculates spermatozoa (n = 3) from an individual boar (20 Landrace, 20 Yorkshire, and 20 Duroc) was evaluated as well as overall accuracy. Results The acrosome reacted (AR) pattern after capacitation (%) was positively correlated with the litter size of Landrace, Yorkshire, and Duroc pigs and the overall accuracy was 75%, 75%, and 70% in Landrace, Yorkshire, and Duroc pigs, respectively. The difference (Δ) in AR pattern before and after capacitation was positively correlated with the litter size of Landrace, Yorkshire, and Duroc pigs and the overall accuracy was 80%, 65%, and 55% in Landrace, Yorkshire, and Duroc pigs, respectively. However, the difference (Δ) in capacitated (B) pattern before and after capacitation was negatively correlated with the litter size of Landrace pigs and the overall accuracy was 75%. Moreover, average litter size was significantly altered according to different combined H33258/CTC staining parameters. Conclusion These results show that combined H33258/CTC staining may be used to predict male fertility in various breeds. However, the selection of specific efficiency combined H33258/CTC staining parameters requires further consideration. Taken together, these findings suggest that combined H33258/CTC staining may constitute an alternative method for predicting male fertility until such time as fertility-related biomarkers are further validated. Litter Size Capacitation Status Boar Spermatozoa Breeds Fertility Animal culture Animal biochemistry Dong-Ha Shin verfasserin aut Do-Yeal Ryu verfasserin aut Amena Khatun verfasserin aut Md Saidur Rahman verfasserin aut Myung-Geol Pang verfasserin aut In Asian-Australasian Journal of Animal Sciences Asian-Australasian Association of Animal Production Societies, 2017 31(2018), 6, Seite 842-850 (DE-627)756825873 (DE-600)2727534-6 19765517 nnns volume:31 year:2018 number:6 pages:842-850 https://doi.org/10.5713/ajas.17.0760 kostenfrei https://doaj.org/article/4f9053579627438286acffb5821a778c kostenfrei http://www.ajas.info/upload/pdf/ajas-31-6-842.pdf kostenfrei https://doaj.org/toc/1011-2367 Journal toc kostenfrei https://doaj.org/toc/1976-5517 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 31 2018 6 842-850
allfieldsGer	10.5713/ajas.17.0760 doi (DE-627)DOAJ075676133 (DE-599)DOAJ4f9053579627438286acffb5821a778c DE-627 ger DE-627 rakwb eng SF1-1100 QP501-801 Woo-Sung Kwon verfasserin aut Applications of capacitation status for litter size enhancement in various pig breeds 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective Several studies have reported the development of new molecular methods for the prognosis and diagnosis of male fertility based on biomarkers aimed at overcoming the limitations of conventional male fertility analysis tools. However, further studies are needed for the field application of these methods. Therefore, alternative methods based on existing semen analysis methods are required to improve production efficiency in the animal industry. Methods we examined the possibility of improving litter size in various pig breeds using combined Hoechst 33258/chlortetracycline fluorescence (H33258/CTC) staining. The correlation between field fertility and capacitation status by combined H33258/CTC staining in different ejaculates spermatozoa (n = 3) from an individual boar (20 Landrace, 20 Yorkshire, and 20 Duroc) was evaluated as well as overall accuracy. Results The acrosome reacted (AR) pattern after capacitation (%) was positively correlated with the litter size of Landrace, Yorkshire, and Duroc pigs and the overall accuracy was 75%, 75%, and 70% in Landrace, Yorkshire, and Duroc pigs, respectively. The difference (Δ) in AR pattern before and after capacitation was positively correlated with the litter size of Landrace, Yorkshire, and Duroc pigs and the overall accuracy was 80%, 65%, and 55% in Landrace, Yorkshire, and Duroc pigs, respectively. However, the difference (Δ) in capacitated (B) pattern before and after capacitation was negatively correlated with the litter size of Landrace pigs and the overall accuracy was 75%. Moreover, average litter size was significantly altered according to different combined H33258/CTC staining parameters. Conclusion These results show that combined H33258/CTC staining may be used to predict male fertility in various breeds. However, the selection of specific efficiency combined H33258/CTC staining parameters requires further consideration. Taken together, these findings suggest that combined H33258/CTC staining may constitute an alternative method for predicting male fertility until such time as fertility-related biomarkers are further validated. Litter Size Capacitation Status Boar Spermatozoa Breeds Fertility Animal culture Animal biochemistry Dong-Ha Shin verfasserin aut Do-Yeal Ryu verfasserin aut Amena Khatun verfasserin aut Md Saidur Rahman verfasserin aut Myung-Geol Pang verfasserin aut In Asian-Australasian Journal of Animal Sciences Asian-Australasian Association of Animal Production Societies, 2017 31(2018), 6, Seite 842-850 (DE-627)756825873 (DE-600)2727534-6 19765517 nnns volume:31 year:2018 number:6 pages:842-850 https://doi.org/10.5713/ajas.17.0760 kostenfrei https://doaj.org/article/4f9053579627438286acffb5821a778c kostenfrei http://www.ajas.info/upload/pdf/ajas-31-6-842.pdf kostenfrei https://doaj.org/toc/1011-2367 Journal toc kostenfrei https://doaj.org/toc/1976-5517 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 31 2018 6 842-850
allfieldsSound	10.5713/ajas.17.0760 doi (DE-627)DOAJ075676133 (DE-599)DOAJ4f9053579627438286acffb5821a778c DE-627 ger DE-627 rakwb eng SF1-1100 QP501-801 Woo-Sung Kwon verfasserin aut Applications of capacitation status for litter size enhancement in various pig breeds 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective Several studies have reported the development of new molecular methods for the prognosis and diagnosis of male fertility based on biomarkers aimed at overcoming the limitations of conventional male fertility analysis tools. However, further studies are needed for the field application of these methods. Therefore, alternative methods based on existing semen analysis methods are required to improve production efficiency in the animal industry. Methods we examined the possibility of improving litter size in various pig breeds using combined Hoechst 33258/chlortetracycline fluorescence (H33258/CTC) staining. The correlation between field fertility and capacitation status by combined H33258/CTC staining in different ejaculates spermatozoa (n = 3) from an individual boar (20 Landrace, 20 Yorkshire, and 20 Duroc) was evaluated as well as overall accuracy. Results The acrosome reacted (AR) pattern after capacitation (%) was positively correlated with the litter size of Landrace, Yorkshire, and Duroc pigs and the overall accuracy was 75%, 75%, and 70% in Landrace, Yorkshire, and Duroc pigs, respectively. The difference (Δ) in AR pattern before and after capacitation was positively correlated with the litter size of Landrace, Yorkshire, and Duroc pigs and the overall accuracy was 80%, 65%, and 55% in Landrace, Yorkshire, and Duroc pigs, respectively. However, the difference (Δ) in capacitated (B) pattern before and after capacitation was negatively correlated with the litter size of Landrace pigs and the overall accuracy was 75%. Moreover, average litter size was significantly altered according to different combined H33258/CTC staining parameters. Conclusion These results show that combined H33258/CTC staining may be used to predict male fertility in various breeds. However, the selection of specific efficiency combined H33258/CTC staining parameters requires further consideration. Taken together, these findings suggest that combined H33258/CTC staining may constitute an alternative method for predicting male fertility until such time as fertility-related biomarkers are further validated. Litter Size Capacitation Status Boar Spermatozoa Breeds Fertility Animal culture Animal biochemistry Dong-Ha Shin verfasserin aut Do-Yeal Ryu verfasserin aut Amena Khatun verfasserin aut Md Saidur Rahman verfasserin aut Myung-Geol Pang verfasserin aut In Asian-Australasian Journal of Animal Sciences Asian-Australasian Association of Animal Production Societies, 2017 31(2018), 6, Seite 842-850 (DE-627)756825873 (DE-600)2727534-6 19765517 nnns volume:31 year:2018 number:6 pages:842-850 https://doi.org/10.5713/ajas.17.0760 kostenfrei https://doaj.org/article/4f9053579627438286acffb5821a778c kostenfrei http://www.ajas.info/upload/pdf/ajas-31-6-842.pdf kostenfrei https://doaj.org/toc/1011-2367 Journal toc kostenfrei https://doaj.org/toc/1976-5517 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 31 2018 6 842-850
language	English
source	In Asian-Australasian Journal of Animal Sciences 31(2018), 6, Seite 842-850 volume:31 year:2018 number:6 pages:842-850
sourceStr	In Asian-Australasian Journal of Animal Sciences 31(2018), 6, Seite 842-850 volume:31 year:2018 number:6 pages:842-850
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topic_facet	Litter Size Capacitation Status Boar Spermatozoa Breeds Fertility Animal culture Animal biochemistry
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container_title	Asian-Australasian Journal of Animal Sciences
authorswithroles_txt_mv	Woo-Sung Kwon @@aut@@ Dong-Ha Shin @@aut@@ Do-Yeal Ryu @@aut@@ Amena Khatun @@aut@@ Md Saidur Rahman @@aut@@ Myung-Geol Pang @@aut@@
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The difference (Δ) in AR pattern before and after capacitation was positively correlated with the litter size of Landrace, Yorkshire, and Duroc pigs and the overall accuracy was 80%, 65%, and 55% in Landrace, Yorkshire, and Duroc pigs, respectively. However, the difference (Δ) in capacitated (B) pattern before and after capacitation was negatively correlated with the litter size of Landrace pigs and the overall accuracy was 75%. Moreover, average litter size was significantly altered according to different combined H33258/CTC staining parameters. Conclusion These results show that combined H33258/CTC staining may be used to predict male fertility in various breeds. However, the selection of specific efficiency combined H33258/CTC staining parameters requires further consideration. 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topic_title	SF1-1100 QP501-801 Applications of capacitation status for litter size enhancement in various pig breeds Litter Size Capacitation Status Boar Spermatozoa Breeds Fertility
topic	misc SF1-1100 misc QP501-801 misc Litter Size misc Capacitation Status misc Boar Spermatozoa misc Breeds misc Fertility misc Animal culture misc Animal biochemistry
topic_unstemmed	misc SF1-1100 misc QP501-801 misc Litter Size misc Capacitation Status misc Boar Spermatozoa misc Breeds misc Fertility misc Animal culture misc Animal biochemistry
topic_browse	misc SF1-1100 misc QP501-801 misc Litter Size misc Capacitation Status misc Boar Spermatozoa misc Breeds misc Fertility misc Animal culture misc Animal biochemistry
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hierarchy_parent_title	Asian-Australasian Journal of Animal Sciences
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title	Applications of capacitation status for litter size enhancement in various pig breeds
ctrlnum	(DE-627)DOAJ075676133 (DE-599)DOAJ4f9053579627438286acffb5821a778c
title_full	Applications of capacitation status for litter size enhancement in various pig breeds
author_sort	Woo-Sung Kwon
journal	Asian-Australasian Journal of Animal Sciences
journalStr	Asian-Australasian Journal of Animal Sciences
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author_browse	Woo-Sung Kwon Dong-Ha Shin Do-Yeal Ryu Amena Khatun Md Saidur Rahman Myung-Geol Pang
container_volume	31
class	SF1-1100 QP501-801
format_se	Elektronische Aufsätze
author-letter	Woo-Sung Kwon
doi_str_mv	10.5713/ajas.17.0760
author2-role	verfasserin
title_sort	applications of capacitation status for litter size enhancement in various pig breeds
callnumber	SF1-1100
title_auth	Applications of capacitation status for litter size enhancement in various pig breeds
abstract	Objective Several studies have reported the development of new molecular methods for the prognosis and diagnosis of male fertility based on biomarkers aimed at overcoming the limitations of conventional male fertility analysis tools. However, further studies are needed for the field application of these methods. Therefore, alternative methods based on existing semen analysis methods are required to improve production efficiency in the animal industry. Methods we examined the possibility of improving litter size in various pig breeds using combined Hoechst 33258/chlortetracycline fluorescence (H33258/CTC) staining. The correlation between field fertility and capacitation status by combined H33258/CTC staining in different ejaculates spermatozoa (n = 3) from an individual boar (20 Landrace, 20 Yorkshire, and 20 Duroc) was evaluated as well as overall accuracy. Results The acrosome reacted (AR) pattern after capacitation (%) was positively correlated with the litter size of Landrace, Yorkshire, and Duroc pigs and the overall accuracy was 75%, 75%, and 70% in Landrace, Yorkshire, and Duroc pigs, respectively. The difference (Δ) in AR pattern before and after capacitation was positively correlated with the litter size of Landrace, Yorkshire, and Duroc pigs and the overall accuracy was 80%, 65%, and 55% in Landrace, Yorkshire, and Duroc pigs, respectively. However, the difference (Δ) in capacitated (B) pattern before and after capacitation was negatively correlated with the litter size of Landrace pigs and the overall accuracy was 75%. Moreover, average litter size was significantly altered according to different combined H33258/CTC staining parameters. Conclusion These results show that combined H33258/CTC staining may be used to predict male fertility in various breeds. However, the selection of specific efficiency combined H33258/CTC staining parameters requires further consideration. Taken together, these findings suggest that combined H33258/CTC staining may constitute an alternative method for predicting male fertility until such time as fertility-related biomarkers are further validated.
abstractGer	Objective Several studies have reported the development of new molecular methods for the prognosis and diagnosis of male fertility based on biomarkers aimed at overcoming the limitations of conventional male fertility analysis tools. However, further studies are needed for the field application of these methods. Therefore, alternative methods based on existing semen analysis methods are required to improve production efficiency in the animal industry. Methods we examined the possibility of improving litter size in various pig breeds using combined Hoechst 33258/chlortetracycline fluorescence (H33258/CTC) staining. The correlation between field fertility and capacitation status by combined H33258/CTC staining in different ejaculates spermatozoa (n = 3) from an individual boar (20 Landrace, 20 Yorkshire, and 20 Duroc) was evaluated as well as overall accuracy. Results The acrosome reacted (AR) pattern after capacitation (%) was positively correlated with the litter size of Landrace, Yorkshire, and Duroc pigs and the overall accuracy was 75%, 75%, and 70% in Landrace, Yorkshire, and Duroc pigs, respectively. The difference (Δ) in AR pattern before and after capacitation was positively correlated with the litter size of Landrace, Yorkshire, and Duroc pigs and the overall accuracy was 80%, 65%, and 55% in Landrace, Yorkshire, and Duroc pigs, respectively. However, the difference (Δ) in capacitated (B) pattern before and after capacitation was negatively correlated with the litter size of Landrace pigs and the overall accuracy was 75%. Moreover, average litter size was significantly altered according to different combined H33258/CTC staining parameters. Conclusion These results show that combined H33258/CTC staining may be used to predict male fertility in various breeds. However, the selection of specific efficiency combined H33258/CTC staining parameters requires further consideration. Taken together, these findings suggest that combined H33258/CTC staining may constitute an alternative method for predicting male fertility until such time as fertility-related biomarkers are further validated.
abstract_unstemmed	Objective Several studies have reported the development of new molecular methods for the prognosis and diagnosis of male fertility based on biomarkers aimed at overcoming the limitations of conventional male fertility analysis tools. However, further studies are needed for the field application of these methods. Therefore, alternative methods based on existing semen analysis methods are required to improve production efficiency in the animal industry. Methods we examined the possibility of improving litter size in various pig breeds using combined Hoechst 33258/chlortetracycline fluorescence (H33258/CTC) staining. The correlation between field fertility and capacitation status by combined H33258/CTC staining in different ejaculates spermatozoa (n = 3) from an individual boar (20 Landrace, 20 Yorkshire, and 20 Duroc) was evaluated as well as overall accuracy. Results The acrosome reacted (AR) pattern after capacitation (%) was positively correlated with the litter size of Landrace, Yorkshire, and Duroc pigs and the overall accuracy was 75%, 75%, and 70% in Landrace, Yorkshire, and Duroc pigs, respectively. The difference (Δ) in AR pattern before and after capacitation was positively correlated with the litter size of Landrace, Yorkshire, and Duroc pigs and the overall accuracy was 80%, 65%, and 55% in Landrace, Yorkshire, and Duroc pigs, respectively. However, the difference (Δ) in capacitated (B) pattern before and after capacitation was negatively correlated with the litter size of Landrace pigs and the overall accuracy was 75%. Moreover, average litter size was significantly altered according to different combined H33258/CTC staining parameters. Conclusion These results show that combined H33258/CTC staining may be used to predict male fertility in various breeds. However, the selection of specific efficiency combined H33258/CTC staining parameters requires further consideration. Taken together, these findings suggest that combined H33258/CTC staining may constitute an alternative method for predicting male fertility until such time as fertility-related biomarkers are further validated.
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title_short	Applications of capacitation status for litter size enhancement in various pig breeds
url	https://doi.org/10.5713/ajas.17.0760 https://doaj.org/article/4f9053579627438286acffb5821a778c http://www.ajas.info/upload/pdf/ajas-31-6-842.pdf https://doaj.org/toc/1011-2367 https://doaj.org/toc/1976-5517
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author2	Dong-Ha Shin Do-Yeal Ryu Amena Khatun Md Saidur Rahman Myung-Geol Pang
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up_date	2024-07-03T16:13:24.943Z
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