Serial femtosecond crystallography of soluble proteins in lipidic cubic phase

Serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs) enables high-resolution protein structure determination using micrometre-sized crystals at room temperature with minimal effects from radiation damage. SFX requires a steady supply of microcrystals intersecting the XFEL b...
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Autor*in:	Raimund Fromme [verfasserIn] Andrii Ishchenko [verfasserIn] Markus Metz [verfasserIn] Shatabdi Roy Chowdhury [verfasserIn] Shibom Basu [verfasserIn] Sébastien Boutet [verfasserIn] Petra Fromme [verfasserIn] Thomas A. White [verfasserIn] Anton Barty [verfasserIn] John C. H. Spence [verfasserIn] Uwe Weierstall [verfasserIn] Wei Liu [verfasserIn] Vadim Cherezov [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2015

Schlagwörter:	serial femtosecond crystallography X-ray free-electron laser lipidic cubic phase soluble protein

Übergeordnetes Werk:	In: IUCrJ - International Union of Crystallography, 2014, 2(2015), 5, Seite 545-551
Übergeordnetes Werk:	volume:2 ; year:2015 ; number:5 ; pages:545-551

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1107/S2052252515013160

Katalog-ID:	DOAJ075837854

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callnumber-first	Q - Science
author	Raimund Fromme
spellingShingle	Raimund Fromme misc QD901-999 misc serial femtosecond crystallography misc X-ray free-electron laser misc lipidic cubic phase misc soluble protein misc Crystallography Serial femtosecond crystallography of soluble proteins in lipidic cubic phase
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topic_title	QD901-999 Serial femtosecond crystallography of soluble proteins in lipidic cubic phase serial femtosecond crystallography X-ray free-electron laser lipidic cubic phase soluble protein
topic	misc QD901-999 misc serial femtosecond crystallography misc X-ray free-electron laser misc lipidic cubic phase misc soluble protein misc Crystallography
topic_unstemmed	misc QD901-999 misc serial femtosecond crystallography misc X-ray free-electron laser misc lipidic cubic phase misc soluble protein misc Crystallography
topic_browse	misc QD901-999 misc serial femtosecond crystallography misc X-ray free-electron laser misc lipidic cubic phase misc soluble protein misc Crystallography
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title	Serial femtosecond crystallography of soluble proteins in lipidic cubic phase
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title_full	Serial femtosecond crystallography of soluble proteins in lipidic cubic phase
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author_browse	Raimund Fromme Andrii Ishchenko Markus Metz Shatabdi Roy Chowdhury Shibom Basu Sébastien Boutet Petra Fromme Thomas A. White Anton Barty John C. H. Spence Uwe Weierstall Wei Liu Vadim Cherezov
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title_sort	serial femtosecond crystallography of soluble proteins in lipidic cubic phase
callnumber	QD901-999
title_auth	Serial femtosecond crystallography of soluble proteins in lipidic cubic phase
abstract	Serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs) enables high-resolution protein structure determination using micrometre-sized crystals at room temperature with minimal effects from radiation damage. SFX requires a steady supply of microcrystals intersecting the XFEL beam at random orientations. An LCP–SFX method has recently been introduced in which microcrystals of membrane proteins are grown and delivered for SFX data collection inside a gel-like membrane-mimetic matrix, known as lipidic cubic phase (LCP), using a special LCP microextrusion injector. Here, it is demonstrated that LCP can also be used as a suitable carrier medium for microcrystals of soluble proteins, enabling a dramatic reduction in the amount of crystallized protein required for data collection compared with crystals delivered by liquid injectors. High-quality LCP–SFX data sets were collected for two soluble proteins, lysozyme and phycocyanin, using less than 0.1 mg of each protein.
abstractGer	Serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs) enables high-resolution protein structure determination using micrometre-sized crystals at room temperature with minimal effects from radiation damage. SFX requires a steady supply of microcrystals intersecting the XFEL beam at random orientations. An LCP–SFX method has recently been introduced in which microcrystals of membrane proteins are grown and delivered for SFX data collection inside a gel-like membrane-mimetic matrix, known as lipidic cubic phase (LCP), using a special LCP microextrusion injector. Here, it is demonstrated that LCP can also be used as a suitable carrier medium for microcrystals of soluble proteins, enabling a dramatic reduction in the amount of crystallized protein required for data collection compared with crystals delivered by liquid injectors. High-quality LCP–SFX data sets were collected for two soluble proteins, lysozyme and phycocyanin, using less than 0.1 mg of each protein.
abstract_unstemmed	Serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs) enables high-resolution protein structure determination using micrometre-sized crystals at room temperature with minimal effects from radiation damage. SFX requires a steady supply of microcrystals intersecting the XFEL beam at random orientations. An LCP–SFX method has recently been introduced in which microcrystals of membrane proteins are grown and delivered for SFX data collection inside a gel-like membrane-mimetic matrix, known as lipidic cubic phase (LCP), using a special LCP microextrusion injector. Here, it is demonstrated that LCP can also be used as a suitable carrier medium for microcrystals of soluble proteins, enabling a dramatic reduction in the amount of crystallized protein required for data collection compared with crystals delivered by liquid injectors. High-quality LCP–SFX data sets were collected for two soluble proteins, lysozyme and phycocyanin, using less than 0.1 mg of each protein.
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title_short	Serial femtosecond crystallography of soluble proteins in lipidic cubic phase
url	https://doi.org/10.1107/S2052252515013160 https://doaj.org/article/edceb6aa19b247f3a5c91329a740b0a7 http://scripts.iucr.org/cgi-bin/paper?S2052252515013160 https://doaj.org/toc/2052-2525
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author2	Andrii Ishchenko Markus Metz Shatabdi Roy Chowdhury Shibom Basu Sébastien Boutet Petra Fromme Thomas A. White Anton Barty John C. H. Spence Uwe Weierstall Wei Liu Vadim Cherezov
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up_date	2024-07-03T17:06:43.378Z
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Serial femtosecond crystallography of soluble proteins in lipidic cubic phase

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