A Linear Surface Epitope in a Proline-Rich Region of ORF3 Product of Genotype 1 Hepatitis E Virus

Hepatitis E virus (HEV) is one of the viral pathogens causing hepatitis in humans. HEV open reading frame 3 (ORF3) encodes a small multifunctional protein (VP13), which is essential for HEV infection. In this study, a linear epitope was identified in a polyproline (PXXP) motif from VP13 of genotype...
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Gespeichert in:

Autor*in:	Yonglin Yang [verfasserIn] Shaoli Lin [verfasserIn] Yuchen Nan [verfasserIn] Zexu Ma [verfasserIn] Liping Yang [verfasserIn] Yanjin Zhang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	hepatitis E virus HEV ORF3 VP13 PXXP motif linear epitope monoclonal antibody

Übergeordnetes Werk:	In: Viruses - MDPI AG, 2009, 8(2016), 8, p 227
Übergeordnetes Werk:	volume:8 ; year:2016 ; number:8, p 227

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/v8080227

Katalog-ID:	DOAJ076386597

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520			\|a Hepatitis E virus (HEV) is one of the viral pathogens causing hepatitis in humans. HEV open reading frame 3 (ORF3) encodes a small multifunctional protein (VP13), which is essential for HEV infection. In this study, a linear epitope was identified in a polyproline (PXXP) motif from VP13 of genotype 1 HEV by using a monoclonal antibody. The epitope was detected in enzyme-linked immunosorbent assay (ELISA), immunoblotting and immunofluorescence assays. Epitope mapping showed that the epitope locates in a proline-rich region containing a PXXP motif in amino acid residues 66-75 of VP13. The epitope was also detected in HEV-infected liver cells and reacted with genotype 1-specific antibodies in an HEV-positive human serum sample. The results demonstrated that the epitope in the PXXP motif of the genotype 1 VP13 is linear and surface-oriented, which should facilitate in-depth studies on the viral protein and HEV biology.
650		4	\|a hepatitis E virus
650		4	\|a HEV
650		4	\|a ORF3
650		4	\|a VP13
650		4	\|a PXXP motif
650		4	\|a linear epitope
650		4	\|a monoclonal antibody
653		0	\|a Microbiology
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912			\|a GBV_ILN_63
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912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
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912			\|a GBV_ILN_4305
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912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
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951			\|a AR
952			\|d 8 \|j 2016 \|e 8, p 227

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allfields	10.3390/v8080227 doi (DE-627)DOAJ076386597 (DE-599)DOAJce4e687615a94d5d848d60ed424e6c65 DE-627 ger DE-627 rakwb eng QR1-502 Yonglin Yang verfasserin aut A Linear Surface Epitope in a Proline-Rich Region of ORF3 Product of Genotype 1 Hepatitis E Virus 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Hepatitis E virus (HEV) is one of the viral pathogens causing hepatitis in humans. HEV open reading frame 3 (ORF3) encodes a small multifunctional protein (VP13), which is essential for HEV infection. In this study, a linear epitope was identified in a polyproline (PXXP) motif from VP13 of genotype 1 HEV by using a monoclonal antibody. The epitope was detected in enzyme-linked immunosorbent assay (ELISA), immunoblotting and immunofluorescence assays. Epitope mapping showed that the epitope locates in a proline-rich region containing a PXXP motif in amino acid residues 66-75 of VP13. The epitope was also detected in HEV-infected liver cells and reacted with genotype 1-specific antibodies in an HEV-positive human serum sample. The results demonstrated that the epitope in the PXXP motif of the genotype 1 VP13 is linear and surface-oriented, which should facilitate in-depth studies on the viral protein and HEV biology. hepatitis E virus HEV ORF3 VP13 PXXP motif linear epitope monoclonal antibody Microbiology Shaoli Lin verfasserin aut Yuchen Nan verfasserin aut Zexu Ma verfasserin aut Liping Yang verfasserin aut Yanjin Zhang verfasserin aut In Viruses MDPI AG, 2009 8(2016), 8, p 227 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:8 year:2016 number:8, p 227 https://doi.org/10.3390/v8080227 kostenfrei https://doaj.org/article/ce4e687615a94d5d848d60ed424e6c65 kostenfrei http://www.mdpi.com/1999-4915/8/8/227 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2016 8, p 227
spelling	10.3390/v8080227 doi (DE-627)DOAJ076386597 (DE-599)DOAJce4e687615a94d5d848d60ed424e6c65 DE-627 ger DE-627 rakwb eng QR1-502 Yonglin Yang verfasserin aut A Linear Surface Epitope in a Proline-Rich Region of ORF3 Product of Genotype 1 Hepatitis E Virus 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Hepatitis E virus (HEV) is one of the viral pathogens causing hepatitis in humans. HEV open reading frame 3 (ORF3) encodes a small multifunctional protein (VP13), which is essential for HEV infection. In this study, a linear epitope was identified in a polyproline (PXXP) motif from VP13 of genotype 1 HEV by using a monoclonal antibody. The epitope was detected in enzyme-linked immunosorbent assay (ELISA), immunoblotting and immunofluorescence assays. Epitope mapping showed that the epitope locates in a proline-rich region containing a PXXP motif in amino acid residues 66-75 of VP13. The epitope was also detected in HEV-infected liver cells and reacted with genotype 1-specific antibodies in an HEV-positive human serum sample. The results demonstrated that the epitope in the PXXP motif of the genotype 1 VP13 is linear and surface-oriented, which should facilitate in-depth studies on the viral protein and HEV biology. hepatitis E virus HEV ORF3 VP13 PXXP motif linear epitope monoclonal antibody Microbiology Shaoli Lin verfasserin aut Yuchen Nan verfasserin aut Zexu Ma verfasserin aut Liping Yang verfasserin aut Yanjin Zhang verfasserin aut In Viruses MDPI AG, 2009 8(2016), 8, p 227 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:8 year:2016 number:8, p 227 https://doi.org/10.3390/v8080227 kostenfrei https://doaj.org/article/ce4e687615a94d5d848d60ed424e6c65 kostenfrei http://www.mdpi.com/1999-4915/8/8/227 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2016 8, p 227
allfields_unstemmed	10.3390/v8080227 doi (DE-627)DOAJ076386597 (DE-599)DOAJce4e687615a94d5d848d60ed424e6c65 DE-627 ger DE-627 rakwb eng QR1-502 Yonglin Yang verfasserin aut A Linear Surface Epitope in a Proline-Rich Region of ORF3 Product of Genotype 1 Hepatitis E Virus 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Hepatitis E virus (HEV) is one of the viral pathogens causing hepatitis in humans. HEV open reading frame 3 (ORF3) encodes a small multifunctional protein (VP13), which is essential for HEV infection. In this study, a linear epitope was identified in a polyproline (PXXP) motif from VP13 of genotype 1 HEV by using a monoclonal antibody. The epitope was detected in enzyme-linked immunosorbent assay (ELISA), immunoblotting and immunofluorescence assays. Epitope mapping showed that the epitope locates in a proline-rich region containing a PXXP motif in amino acid residues 66-75 of VP13. The epitope was also detected in HEV-infected liver cells and reacted with genotype 1-specific antibodies in an HEV-positive human serum sample. The results demonstrated that the epitope in the PXXP motif of the genotype 1 VP13 is linear and surface-oriented, which should facilitate in-depth studies on the viral protein and HEV biology. hepatitis E virus HEV ORF3 VP13 PXXP motif linear epitope monoclonal antibody Microbiology Shaoli Lin verfasserin aut Yuchen Nan verfasserin aut Zexu Ma verfasserin aut Liping Yang verfasserin aut Yanjin Zhang verfasserin aut In Viruses MDPI AG, 2009 8(2016), 8, p 227 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:8 year:2016 number:8, p 227 https://doi.org/10.3390/v8080227 kostenfrei https://doaj.org/article/ce4e687615a94d5d848d60ed424e6c65 kostenfrei http://www.mdpi.com/1999-4915/8/8/227 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2016 8, p 227
allfieldsGer	10.3390/v8080227 doi (DE-627)DOAJ076386597 (DE-599)DOAJce4e687615a94d5d848d60ed424e6c65 DE-627 ger DE-627 rakwb eng QR1-502 Yonglin Yang verfasserin aut A Linear Surface Epitope in a Proline-Rich Region of ORF3 Product of Genotype 1 Hepatitis E Virus 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Hepatitis E virus (HEV) is one of the viral pathogens causing hepatitis in humans. HEV open reading frame 3 (ORF3) encodes a small multifunctional protein (VP13), which is essential for HEV infection. In this study, a linear epitope was identified in a polyproline (PXXP) motif from VP13 of genotype 1 HEV by using a monoclonal antibody. The epitope was detected in enzyme-linked immunosorbent assay (ELISA), immunoblotting and immunofluorescence assays. Epitope mapping showed that the epitope locates in a proline-rich region containing a PXXP motif in amino acid residues 66-75 of VP13. The epitope was also detected in HEV-infected liver cells and reacted with genotype 1-specific antibodies in an HEV-positive human serum sample. The results demonstrated that the epitope in the PXXP motif of the genotype 1 VP13 is linear and surface-oriented, which should facilitate in-depth studies on the viral protein and HEV biology. hepatitis E virus HEV ORF3 VP13 PXXP motif linear epitope monoclonal antibody Microbiology Shaoli Lin verfasserin aut Yuchen Nan verfasserin aut Zexu Ma verfasserin aut Liping Yang verfasserin aut Yanjin Zhang verfasserin aut In Viruses MDPI AG, 2009 8(2016), 8, p 227 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:8 year:2016 number:8, p 227 https://doi.org/10.3390/v8080227 kostenfrei https://doaj.org/article/ce4e687615a94d5d848d60ed424e6c65 kostenfrei http://www.mdpi.com/1999-4915/8/8/227 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2016 8, p 227
allfieldsSound	10.3390/v8080227 doi (DE-627)DOAJ076386597 (DE-599)DOAJce4e687615a94d5d848d60ed424e6c65 DE-627 ger DE-627 rakwb eng QR1-502 Yonglin Yang verfasserin aut A Linear Surface Epitope in a Proline-Rich Region of ORF3 Product of Genotype 1 Hepatitis E Virus 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Hepatitis E virus (HEV) is one of the viral pathogens causing hepatitis in humans. HEV open reading frame 3 (ORF3) encodes a small multifunctional protein (VP13), which is essential for HEV infection. In this study, a linear epitope was identified in a polyproline (PXXP) motif from VP13 of genotype 1 HEV by using a monoclonal antibody. The epitope was detected in enzyme-linked immunosorbent assay (ELISA), immunoblotting and immunofluorescence assays. Epitope mapping showed that the epitope locates in a proline-rich region containing a PXXP motif in amino acid residues 66-75 of VP13. The epitope was also detected in HEV-infected liver cells and reacted with genotype 1-specific antibodies in an HEV-positive human serum sample. The results demonstrated that the epitope in the PXXP motif of the genotype 1 VP13 is linear and surface-oriented, which should facilitate in-depth studies on the viral protein and HEV biology. hepatitis E virus HEV ORF3 VP13 PXXP motif linear epitope monoclonal antibody Microbiology Shaoli Lin verfasserin aut Yuchen Nan verfasserin aut Zexu Ma verfasserin aut Liping Yang verfasserin aut Yanjin Zhang verfasserin aut In Viruses MDPI AG, 2009 8(2016), 8, p 227 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:8 year:2016 number:8, p 227 https://doi.org/10.3390/v8080227 kostenfrei https://doaj.org/article/ce4e687615a94d5d848d60ed424e6c65 kostenfrei http://www.mdpi.com/1999-4915/8/8/227 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2016 8, p 227
language	English
source	In Viruses 8(2016), 8, p 227 volume:8 year:2016 number:8, p 227
sourceStr	In Viruses 8(2016), 8, p 227 volume:8 year:2016 number:8, p 227
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	hepatitis E virus HEV ORF3 VP13 PXXP motif linear epitope monoclonal antibody Microbiology
isfreeaccess_bool	true
container_title	Viruses
authorswithroles_txt_mv	Yonglin Yang @@aut@@ Shaoli Lin @@aut@@ Yuchen Nan @@aut@@ Zexu Ma @@aut@@ Liping Yang @@aut@@ Yanjin Zhang @@aut@@
publishDateDaySort_date	2016-01-01T00:00:00Z
hierarchy_top_id	609775871
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callnumber-first	Q - Science
author	Yonglin Yang
spellingShingle	Yonglin Yang misc QR1-502 misc hepatitis E virus misc HEV misc ORF3 misc VP13 misc PXXP motif misc linear epitope misc monoclonal antibody misc Microbiology A Linear Surface Epitope in a Proline-Rich Region of ORF3 Product of Genotype 1 Hepatitis E Virus
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topic_title	QR1-502 A Linear Surface Epitope in a Proline-Rich Region of ORF3 Product of Genotype 1 Hepatitis E Virus hepatitis E virus HEV ORF3 VP13 PXXP motif linear epitope monoclonal antibody
topic	misc QR1-502 misc hepatitis E virus misc HEV misc ORF3 misc VP13 misc PXXP motif misc linear epitope misc monoclonal antibody misc Microbiology
topic_unstemmed	misc QR1-502 misc hepatitis E virus misc HEV misc ORF3 misc VP13 misc PXXP motif misc linear epitope misc monoclonal antibody misc Microbiology
topic_browse	misc QR1-502 misc hepatitis E virus misc HEV misc ORF3 misc VP13 misc PXXP motif misc linear epitope misc monoclonal antibody misc Microbiology
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title	A Linear Surface Epitope in a Proline-Rich Region of ORF3 Product of Genotype 1 Hepatitis E Virus
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title_full	A Linear Surface Epitope in a Proline-Rich Region of ORF3 Product of Genotype 1 Hepatitis E Virus
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title_sort	linear surface epitope in a proline-rich region of orf3 product of genotype 1 hepatitis e virus
callnumber	QR1-502
title_auth	A Linear Surface Epitope in a Proline-Rich Region of ORF3 Product of Genotype 1 Hepatitis E Virus
abstract	Hepatitis E virus (HEV) is one of the viral pathogens causing hepatitis in humans. HEV open reading frame 3 (ORF3) encodes a small multifunctional protein (VP13), which is essential for HEV infection. In this study, a linear epitope was identified in a polyproline (PXXP) motif from VP13 of genotype 1 HEV by using a monoclonal antibody. The epitope was detected in enzyme-linked immunosorbent assay (ELISA), immunoblotting and immunofluorescence assays. Epitope mapping showed that the epitope locates in a proline-rich region containing a PXXP motif in amino acid residues 66-75 of VP13. The epitope was also detected in HEV-infected liver cells and reacted with genotype 1-specific antibodies in an HEV-positive human serum sample. The results demonstrated that the epitope in the PXXP motif of the genotype 1 VP13 is linear and surface-oriented, which should facilitate in-depth studies on the viral protein and HEV biology.
abstractGer	Hepatitis E virus (HEV) is one of the viral pathogens causing hepatitis in humans. HEV open reading frame 3 (ORF3) encodes a small multifunctional protein (VP13), which is essential for HEV infection. In this study, a linear epitope was identified in a polyproline (PXXP) motif from VP13 of genotype 1 HEV by using a monoclonal antibody. The epitope was detected in enzyme-linked immunosorbent assay (ELISA), immunoblotting and immunofluorescence assays. Epitope mapping showed that the epitope locates in a proline-rich region containing a PXXP motif in amino acid residues 66-75 of VP13. The epitope was also detected in HEV-infected liver cells and reacted with genotype 1-specific antibodies in an HEV-positive human serum sample. The results demonstrated that the epitope in the PXXP motif of the genotype 1 VP13 is linear and surface-oriented, which should facilitate in-depth studies on the viral protein and HEV biology.
abstract_unstemmed	Hepatitis E virus (HEV) is one of the viral pathogens causing hepatitis in humans. HEV open reading frame 3 (ORF3) encodes a small multifunctional protein (VP13), which is essential for HEV infection. In this study, a linear epitope was identified in a polyproline (PXXP) motif from VP13 of genotype 1 HEV by using a monoclonal antibody. The epitope was detected in enzyme-linked immunosorbent assay (ELISA), immunoblotting and immunofluorescence assays. Epitope mapping showed that the epitope locates in a proline-rich region containing a PXXP motif in amino acid residues 66-75 of VP13. The epitope was also detected in HEV-infected liver cells and reacted with genotype 1-specific antibodies in an HEV-positive human serum sample. The results demonstrated that the epitope in the PXXP motif of the genotype 1 VP13 is linear and surface-oriented, which should facilitate in-depth studies on the viral protein and HEV biology.
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container_issue	8, p 227
title_short	A Linear Surface Epitope in a Proline-Rich Region of ORF3 Product of Genotype 1 Hepatitis E Virus
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A Linear Surface Epitope in a Proline-Rich Region of ORF3 Product of Genotype 1 Hepatitis E Virus

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