Dental Pulp Stem Cells from Natal Teeth: Isolation and Morphological Study
Introduction: Stem cells have a remarkable capacity for selfregeneration and have the potential to originate different types of cells and tissue. There is a significant occurrence of natal teeth in newborn babies and usually the treatment consists of surgical removal. Aim: To isolate and extensively...
Ausführliche Beschreibung
Autor*in: |
Karla Mayra Rezende [verfasserIn] José Carlos P Imparato [verfasserIn] Daniela Carvalho De Oliveira França [verfasserIn] Marcone Oliveira Rocha [verfasserIn] Marcelo Bönecker [verfasserIn] |
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E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2018 |
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Übergeordnetes Werk: |
In: Journal of Clinical and Diagnostic Research - JCDR Research and Publications Private Limited, 2009, 12(2018), 3, Seite ZC46-ZC49 |
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Übergeordnetes Werk: |
volume:12 ; year:2018 ; number:3 ; pages:ZC46-ZC49 |
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Link aufrufen |
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DOI / URN: |
10.7860/JCDR/2018/30783.11322 |
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Katalog-ID: |
DOAJ076496341 |
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10.7860/JCDR/2018/30783.11322 doi (DE-627)DOAJ076496341 (DE-599)DOAJe37a47fd3ea24384917966659cd6c174 DE-627 ger DE-627 rakwb eng Karla Mayra Rezende verfasserin aut Dental Pulp Stem Cells from Natal Teeth: Isolation and Morphological Study 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: Stem cells have a remarkable capacity for selfregeneration and have the potential to originate different types of cells and tissue. There is a significant occurrence of natal teeth in newborn babies and usually the treatment consists of surgical removal. Aim: To isolate and extensively characterise stem cells derived from human natal dental pulp. For this characterisation, proliferation capacity, ultrastructural morphological evaluation and trace elements were utilised. Materials and Methods: The study was carried out in the oral pathology laboratory during October 2016. Cells from the pulp of two natal teeth were isolated through the explant technique and separated with a STRO-1 marker. The colony forming units, cell proliferation and cell viability after plating and the growth curve were analysed. The cells were morphologically analysed through Scanning Electron Microscopy (SEM) and the trace elements were analysed using Energy Dispersive Spectroscopy (EDS). Results: The predominant cellular morphology, observed in the stem cells separated with the STRO-1 biological marker, was fibroblastic. The study of trace elements using EDS detected chlorine, sodium and sulfur. Conclusion: Natal teeth extracted for medical reasons could be an opportunity for everyone to preserve stem cells, permitting their use in future experimental studies. cell culture colony forming assay cell viability test Medicine R José Carlos P Imparato verfasserin aut Daniela Carvalho De Oliveira França verfasserin aut Marcone Oliveira Rocha verfasserin aut Marcelo Bönecker verfasserin aut In Journal of Clinical and Diagnostic Research JCDR Research and Publications Private Limited, 2009 12(2018), 3, Seite ZC46-ZC49 (DE-627)789478048 (DE-600)2775283-5 0973709X nnns volume:12 year:2018 number:3 pages:ZC46-ZC49 https://doi.org/10.7860/JCDR/2018/30783.11322 kostenfrei https://doaj.org/article/e37a47fd3ea24384917966659cd6c174 kostenfrei https://jcdr.net/articles/PDF/11322/30783_280218_30783_CE[Ra1]%20corrected_F(SL)_PF1(EK_SL)_PFA(EK_SL)_PB(EK_SL)_PN(AP).pdf kostenfrei https://doaj.org/toc/2249-782X Journal toc kostenfrei https://doaj.org/toc/0973-709X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2018 3 ZC46-ZC49 |
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10.7860/JCDR/2018/30783.11322 doi (DE-627)DOAJ076496341 (DE-599)DOAJe37a47fd3ea24384917966659cd6c174 DE-627 ger DE-627 rakwb eng Karla Mayra Rezende verfasserin aut Dental Pulp Stem Cells from Natal Teeth: Isolation and Morphological Study 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: Stem cells have a remarkable capacity for selfregeneration and have the potential to originate different types of cells and tissue. There is a significant occurrence of natal teeth in newborn babies and usually the treatment consists of surgical removal. Aim: To isolate and extensively characterise stem cells derived from human natal dental pulp. For this characterisation, proliferation capacity, ultrastructural morphological evaluation and trace elements were utilised. Materials and Methods: The study was carried out in the oral pathology laboratory during October 2016. Cells from the pulp of two natal teeth were isolated through the explant technique and separated with a STRO-1 marker. The colony forming units, cell proliferation and cell viability after plating and the growth curve were analysed. The cells were morphologically analysed through Scanning Electron Microscopy (SEM) and the trace elements were analysed using Energy Dispersive Spectroscopy (EDS). Results: The predominant cellular morphology, observed in the stem cells separated with the STRO-1 biological marker, was fibroblastic. The study of trace elements using EDS detected chlorine, sodium and sulfur. Conclusion: Natal teeth extracted for medical reasons could be an opportunity for everyone to preserve stem cells, permitting their use in future experimental studies. cell culture colony forming assay cell viability test Medicine R José Carlos P Imparato verfasserin aut Daniela Carvalho De Oliveira França verfasserin aut Marcone Oliveira Rocha verfasserin aut Marcelo Bönecker verfasserin aut In Journal of Clinical and Diagnostic Research JCDR Research and Publications Private Limited, 2009 12(2018), 3, Seite ZC46-ZC49 (DE-627)789478048 (DE-600)2775283-5 0973709X nnns volume:12 year:2018 number:3 pages:ZC46-ZC49 https://doi.org/10.7860/JCDR/2018/30783.11322 kostenfrei https://doaj.org/article/e37a47fd3ea24384917966659cd6c174 kostenfrei https://jcdr.net/articles/PDF/11322/30783_280218_30783_CE[Ra1]%20corrected_F(SL)_PF1(EK_SL)_PFA(EK_SL)_PB(EK_SL)_PN(AP).pdf kostenfrei https://doaj.org/toc/2249-782X Journal toc kostenfrei https://doaj.org/toc/0973-709X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2018 3 ZC46-ZC49 |
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10.7860/JCDR/2018/30783.11322 doi (DE-627)DOAJ076496341 (DE-599)DOAJe37a47fd3ea24384917966659cd6c174 DE-627 ger DE-627 rakwb eng Karla Mayra Rezende verfasserin aut Dental Pulp Stem Cells from Natal Teeth: Isolation and Morphological Study 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: Stem cells have a remarkable capacity for selfregeneration and have the potential to originate different types of cells and tissue. There is a significant occurrence of natal teeth in newborn babies and usually the treatment consists of surgical removal. Aim: To isolate and extensively characterise stem cells derived from human natal dental pulp. For this characterisation, proliferation capacity, ultrastructural morphological evaluation and trace elements were utilised. Materials and Methods: The study was carried out in the oral pathology laboratory during October 2016. Cells from the pulp of two natal teeth were isolated through the explant technique and separated with a STRO-1 marker. The colony forming units, cell proliferation and cell viability after plating and the growth curve were analysed. The cells were morphologically analysed through Scanning Electron Microscopy (SEM) and the trace elements were analysed using Energy Dispersive Spectroscopy (EDS). Results: The predominant cellular morphology, observed in the stem cells separated with the STRO-1 biological marker, was fibroblastic. The study of trace elements using EDS detected chlorine, sodium and sulfur. Conclusion: Natal teeth extracted for medical reasons could be an opportunity for everyone to preserve stem cells, permitting their use in future experimental studies. cell culture colony forming assay cell viability test Medicine R José Carlos P Imparato verfasserin aut Daniela Carvalho De Oliveira França verfasserin aut Marcone Oliveira Rocha verfasserin aut Marcelo Bönecker verfasserin aut In Journal of Clinical and Diagnostic Research JCDR Research and Publications Private Limited, 2009 12(2018), 3, Seite ZC46-ZC49 (DE-627)789478048 (DE-600)2775283-5 0973709X nnns volume:12 year:2018 number:3 pages:ZC46-ZC49 https://doi.org/10.7860/JCDR/2018/30783.11322 kostenfrei https://doaj.org/article/e37a47fd3ea24384917966659cd6c174 kostenfrei https://jcdr.net/articles/PDF/11322/30783_280218_30783_CE[Ra1]%20corrected_F(SL)_PF1(EK_SL)_PFA(EK_SL)_PB(EK_SL)_PN(AP).pdf kostenfrei https://doaj.org/toc/2249-782X Journal toc kostenfrei https://doaj.org/toc/0973-709X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2018 3 ZC46-ZC49 |
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10.7860/JCDR/2018/30783.11322 doi (DE-627)DOAJ076496341 (DE-599)DOAJe37a47fd3ea24384917966659cd6c174 DE-627 ger DE-627 rakwb eng Karla Mayra Rezende verfasserin aut Dental Pulp Stem Cells from Natal Teeth: Isolation and Morphological Study 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: Stem cells have a remarkable capacity for selfregeneration and have the potential to originate different types of cells and tissue. There is a significant occurrence of natal teeth in newborn babies and usually the treatment consists of surgical removal. Aim: To isolate and extensively characterise stem cells derived from human natal dental pulp. For this characterisation, proliferation capacity, ultrastructural morphological evaluation and trace elements were utilised. Materials and Methods: The study was carried out in the oral pathology laboratory during October 2016. Cells from the pulp of two natal teeth were isolated through the explant technique and separated with a STRO-1 marker. The colony forming units, cell proliferation and cell viability after plating and the growth curve were analysed. The cells were morphologically analysed through Scanning Electron Microscopy (SEM) and the trace elements were analysed using Energy Dispersive Spectroscopy (EDS). Results: The predominant cellular morphology, observed in the stem cells separated with the STRO-1 biological marker, was fibroblastic. The study of trace elements using EDS detected chlorine, sodium and sulfur. Conclusion: Natal teeth extracted for medical reasons could be an opportunity for everyone to preserve stem cells, permitting their use in future experimental studies. cell culture colony forming assay cell viability test Medicine R José Carlos P Imparato verfasserin aut Daniela Carvalho De Oliveira França verfasserin aut Marcone Oliveira Rocha verfasserin aut Marcelo Bönecker verfasserin aut In Journal of Clinical and Diagnostic Research JCDR Research and Publications Private Limited, 2009 12(2018), 3, Seite ZC46-ZC49 (DE-627)789478048 (DE-600)2775283-5 0973709X nnns volume:12 year:2018 number:3 pages:ZC46-ZC49 https://doi.org/10.7860/JCDR/2018/30783.11322 kostenfrei https://doaj.org/article/e37a47fd3ea24384917966659cd6c174 kostenfrei https://jcdr.net/articles/PDF/11322/30783_280218_30783_CE[Ra1]%20corrected_F(SL)_PF1(EK_SL)_PFA(EK_SL)_PB(EK_SL)_PN(AP).pdf kostenfrei https://doaj.org/toc/2249-782X Journal toc kostenfrei https://doaj.org/toc/0973-709X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2018 3 ZC46-ZC49 |
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10.7860/JCDR/2018/30783.11322 doi (DE-627)DOAJ076496341 (DE-599)DOAJe37a47fd3ea24384917966659cd6c174 DE-627 ger DE-627 rakwb eng Karla Mayra Rezende verfasserin aut Dental Pulp Stem Cells from Natal Teeth: Isolation and Morphological Study 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: Stem cells have a remarkable capacity for selfregeneration and have the potential to originate different types of cells and tissue. There is a significant occurrence of natal teeth in newborn babies and usually the treatment consists of surgical removal. Aim: To isolate and extensively characterise stem cells derived from human natal dental pulp. For this characterisation, proliferation capacity, ultrastructural morphological evaluation and trace elements were utilised. Materials and Methods: The study was carried out in the oral pathology laboratory during October 2016. Cells from the pulp of two natal teeth were isolated through the explant technique and separated with a STRO-1 marker. The colony forming units, cell proliferation and cell viability after plating and the growth curve were analysed. The cells were morphologically analysed through Scanning Electron Microscopy (SEM) and the trace elements were analysed using Energy Dispersive Spectroscopy (EDS). Results: The predominant cellular morphology, observed in the stem cells separated with the STRO-1 biological marker, was fibroblastic. The study of trace elements using EDS detected chlorine, sodium and sulfur. Conclusion: Natal teeth extracted for medical reasons could be an opportunity for everyone to preserve stem cells, permitting their use in future experimental studies. cell culture colony forming assay cell viability test Medicine R José Carlos P Imparato verfasserin aut Daniela Carvalho De Oliveira França verfasserin aut Marcone Oliveira Rocha verfasserin aut Marcelo Bönecker verfasserin aut In Journal of Clinical and Diagnostic Research JCDR Research and Publications Private Limited, 2009 12(2018), 3, Seite ZC46-ZC49 (DE-627)789478048 (DE-600)2775283-5 0973709X nnns volume:12 year:2018 number:3 pages:ZC46-ZC49 https://doi.org/10.7860/JCDR/2018/30783.11322 kostenfrei https://doaj.org/article/e37a47fd3ea24384917966659cd6c174 kostenfrei https://jcdr.net/articles/PDF/11322/30783_280218_30783_CE[Ra1]%20corrected_F(SL)_PF1(EK_SL)_PFA(EK_SL)_PB(EK_SL)_PN(AP).pdf kostenfrei https://doaj.org/toc/2249-782X Journal toc kostenfrei https://doaj.org/toc/0973-709X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2018 3 ZC46-ZC49 |
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Dental Pulp Stem Cells from Natal Teeth: Isolation and Morphological Study |
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Introduction: Stem cells have a remarkable capacity for selfregeneration and have the potential to originate different types of cells and tissue. There is a significant occurrence of natal teeth in newborn babies and usually the treatment consists of surgical removal. Aim: To isolate and extensively characterise stem cells derived from human natal dental pulp. For this characterisation, proliferation capacity, ultrastructural morphological evaluation and trace elements were utilised. Materials and Methods: The study was carried out in the oral pathology laboratory during October 2016. Cells from the pulp of two natal teeth were isolated through the explant technique and separated with a STRO-1 marker. The colony forming units, cell proliferation and cell viability after plating and the growth curve were analysed. The cells were morphologically analysed through Scanning Electron Microscopy (SEM) and the trace elements were analysed using Energy Dispersive Spectroscopy (EDS). Results: The predominant cellular morphology, observed in the stem cells separated with the STRO-1 biological marker, was fibroblastic. The study of trace elements using EDS detected chlorine, sodium and sulfur. Conclusion: Natal teeth extracted for medical reasons could be an opportunity for everyone to preserve stem cells, permitting their use in future experimental studies. |
abstractGer |
Introduction: Stem cells have a remarkable capacity for selfregeneration and have the potential to originate different types of cells and tissue. There is a significant occurrence of natal teeth in newborn babies and usually the treatment consists of surgical removal. Aim: To isolate and extensively characterise stem cells derived from human natal dental pulp. For this characterisation, proliferation capacity, ultrastructural morphological evaluation and trace elements were utilised. Materials and Methods: The study was carried out in the oral pathology laboratory during October 2016. Cells from the pulp of two natal teeth were isolated through the explant technique and separated with a STRO-1 marker. The colony forming units, cell proliferation and cell viability after plating and the growth curve were analysed. The cells were morphologically analysed through Scanning Electron Microscopy (SEM) and the trace elements were analysed using Energy Dispersive Spectroscopy (EDS). Results: The predominant cellular morphology, observed in the stem cells separated with the STRO-1 biological marker, was fibroblastic. The study of trace elements using EDS detected chlorine, sodium and sulfur. Conclusion: Natal teeth extracted for medical reasons could be an opportunity for everyone to preserve stem cells, permitting their use in future experimental studies. |
abstract_unstemmed |
Introduction: Stem cells have a remarkable capacity for selfregeneration and have the potential to originate different types of cells and tissue. There is a significant occurrence of natal teeth in newborn babies and usually the treatment consists of surgical removal. Aim: To isolate and extensively characterise stem cells derived from human natal dental pulp. For this characterisation, proliferation capacity, ultrastructural morphological evaluation and trace elements were utilised. Materials and Methods: The study was carried out in the oral pathology laboratory during October 2016. Cells from the pulp of two natal teeth were isolated through the explant technique and separated with a STRO-1 marker. The colony forming units, cell proliferation and cell viability after plating and the growth curve were analysed. The cells were morphologically analysed through Scanning Electron Microscopy (SEM) and the trace elements were analysed using Energy Dispersive Spectroscopy (EDS). Results: The predominant cellular morphology, observed in the stem cells separated with the STRO-1 biological marker, was fibroblastic. The study of trace elements using EDS detected chlorine, sodium and sulfur. Conclusion: Natal teeth extracted for medical reasons could be an opportunity for everyone to preserve stem cells, permitting their use in future experimental studies. |
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