Superoxide Dismutase: A Biomarker for Early Diagnosis of Tuberculosis
Introduction: Tuberculosis (TB) is a major public health concern in developing countries. Traditional diagnostic methods such as microscopic detection of organism and histopathological examination are limited in their capacity to provide information on prognosis and treatment efficiency. Abundantly...
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| Autor*in: |
Dipak Kumar Chattopadhyay [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2019 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
In: Journal of Clinical and Diagnostic Research - JCDR Research and Publications Private Limited, 2009, 13(2019), 7, Seite BC01-BC03 |
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| Übergeordnetes Werk: |
volume:13 ; year:2019 ; number:7 ; pages:BC01-BC03 |
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Link aufrufen |
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| DOI / URN: |
10.7860/JCDR/2019/35298.12968 |
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| Katalog-ID: |
DOAJ076902382 |
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| 520 | |a Introduction: Tuberculosis (TB) is a major public health concern in developing countries. Traditional diagnostic methods such as microscopic detection of organism and histopathological examination are limited in their capacity to provide information on prognosis and treatment efficiency. Abundantly secreted extracellular Superoxide Dismutase (SOD) protects the Mycobacterium tuberculosis (MTB), and elevated levels of the biomolecule are found in tissues and fluids of tubercular patients. Aim: To estimate the levels of serum SOD in lung control disease and tubercular patients; assess the diagnostic potential of SOD assay for detection of tuberculosis at an early onset stage and quantify the serum SOD activity to monitor the course of infection and determine the effectiveness of the anti-Tubercular (anti-TB) drug treatment. Materials and Methods: The present observational study was conducted on 180 participants at B.S. Medical College and Hospital, Bankura, West Bengal, India. The participants were divided into three groups: Group 1: Normal Control (n=30), Group 2: Disease Control/Lung-Disease Control (n=27) and Group 3: Tubercular Subjects (3A- Pulmonary TB and 3B- Extrapulmonary TB) (n=76). Serum SOD levels of the participants were measured spectrophotometrically. The serum SOD levels of the patients were re-measured after one-month of A-TB drug treatment. In addition, to increase the specificity of the test, the serum of tubercular subjects was incubated with different concentrations of Sodium Cyanide (NaCN) and then assayed for SOD activity. The level of significance was assessed using Student’s t-test. Results: The serum SOD level in tubercular subjects (both pulmonary and extra-pulmonary; with treatment started between 0-15 days) were significantly (p-value <0.01) elevated as compared to the control and lung-disease subjects. Significant decrease in serum SOD levels was observed after one-month of A-TB drug treatment signifying a decrease in mycobacterial load in host tissues. The iron co-factored SOD secreted by M.tuberculosisis was found to be resistant to NaCN whereas Copper-Zinc (Cu-Zn) co-factored SOD was inhibited by NaCN. Conclusion: The serum SOD assay, used in the present study, differentiated between human and mycobacterial origin-SOD, on incubation with NaCN, and diagnosed pulmonary as well as extra-pulmonary TB cases with confidence. Thus, it can be used as a simple, rapid, inexpensive, yet highly sensitive and specific- assay for detection of both human and bovine tuberculosis and primary and secondary drug-resistant cases. | ||
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10.7860/JCDR/2019/35298.12968 doi (DE-627)DOAJ076902382 (DE-599)DOAJ4b68fa3cd6eb44f1968f01eb748993d6 DE-627 ger DE-627 rakwb eng Dipak Kumar Chattopadhyay verfasserin aut Superoxide Dismutase: A Biomarker for Early Diagnosis of Tuberculosis 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: Tuberculosis (TB) is a major public health concern in developing countries. Traditional diagnostic methods such as microscopic detection of organism and histopathological examination are limited in their capacity to provide information on prognosis and treatment efficiency. Abundantly secreted extracellular Superoxide Dismutase (SOD) protects the Mycobacterium tuberculosis (MTB), and elevated levels of the biomolecule are found in tissues and fluids of tubercular patients. Aim: To estimate the levels of serum SOD in lung control disease and tubercular patients; assess the diagnostic potential of SOD assay for detection of tuberculosis at an early onset stage and quantify the serum SOD activity to monitor the course of infection and determine the effectiveness of the anti-Tubercular (anti-TB) drug treatment. Materials and Methods: The present observational study was conducted on 180 participants at B.S. Medical College and Hospital, Bankura, West Bengal, India. The participants were divided into three groups: Group 1: Normal Control (n=30), Group 2: Disease Control/Lung-Disease Control (n=27) and Group 3: Tubercular Subjects (3A- Pulmonary TB and 3B- Extrapulmonary TB) (n=76). Serum SOD levels of the participants were measured spectrophotometrically. The serum SOD levels of the patients were re-measured after one-month of A-TB drug treatment. In addition, to increase the specificity of the test, the serum of tubercular subjects was incubated with different concentrations of Sodium Cyanide (NaCN) and then assayed for SOD activity. The level of significance was assessed using Student’s t-test. Results: The serum SOD level in tubercular subjects (both pulmonary and extra-pulmonary; with treatment started between 0-15 days) were significantly (p-value <0.01) elevated as compared to the control and lung-disease subjects. Significant decrease in serum SOD levels was observed after one-month of A-TB drug treatment signifying a decrease in mycobacterial load in host tissues. The iron co-factored SOD secreted by M.tuberculosisis was found to be resistant to NaCN whereas Copper-Zinc (Cu-Zn) co-factored SOD was inhibited by NaCN. Conclusion: The serum SOD assay, used in the present study, differentiated between human and mycobacterial origin-SOD, on incubation with NaCN, and diagnosed pulmonary as well as extra-pulmonary TB cases with confidence. Thus, it can be used as a simple, rapid, inexpensive, yet highly sensitive and specific- assay for detection of both human and bovine tuberculosis and primary and secondary drug-resistant cases. anti-tuberculosis treatment diagnostic marker extra-pulmonary tuberculosis pulmonary tuberculosis sodium-cyanide Medicine R In Journal of Clinical and Diagnostic Research JCDR Research and Publications Private Limited, 2009 13(2019), 7, Seite BC01-BC03 (DE-627)789478048 (DE-600)2775283-5 0973709X nnns volume:13 year:2019 number:7 pages:BC01-BC03 https://doi.org/10.7860/JCDR/2019/35298.12968 kostenfrei https://doaj.org/article/4b68fa3cd6eb44f1968f01eb748993d6 kostenfrei https://jcdr.net/articles/PDF/12968/35298_PD(V-3_NG_KM)_CE[Ra1]_F(KM)_PF1(AJ_SHU)_PFA(AJ_SHU)_PN(SL).pdf kostenfrei https://doaj.org/toc/2249-782X Journal toc kostenfrei https://doaj.org/toc/0973-709X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2019 7 BC01-BC03 |
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10.7860/JCDR/2019/35298.12968 doi (DE-627)DOAJ076902382 (DE-599)DOAJ4b68fa3cd6eb44f1968f01eb748993d6 DE-627 ger DE-627 rakwb eng Dipak Kumar Chattopadhyay verfasserin aut Superoxide Dismutase: A Biomarker for Early Diagnosis of Tuberculosis 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: Tuberculosis (TB) is a major public health concern in developing countries. Traditional diagnostic methods such as microscopic detection of organism and histopathological examination are limited in their capacity to provide information on prognosis and treatment efficiency. Abundantly secreted extracellular Superoxide Dismutase (SOD) protects the Mycobacterium tuberculosis (MTB), and elevated levels of the biomolecule are found in tissues and fluids of tubercular patients. Aim: To estimate the levels of serum SOD in lung control disease and tubercular patients; assess the diagnostic potential of SOD assay for detection of tuberculosis at an early onset stage and quantify the serum SOD activity to monitor the course of infection and determine the effectiveness of the anti-Tubercular (anti-TB) drug treatment. Materials and Methods: The present observational study was conducted on 180 participants at B.S. Medical College and Hospital, Bankura, West Bengal, India. The participants were divided into three groups: Group 1: Normal Control (n=30), Group 2: Disease Control/Lung-Disease Control (n=27) and Group 3: Tubercular Subjects (3A- Pulmonary TB and 3B- Extrapulmonary TB) (n=76). Serum SOD levels of the participants were measured spectrophotometrically. The serum SOD levels of the patients were re-measured after one-month of A-TB drug treatment. In addition, to increase the specificity of the test, the serum of tubercular subjects was incubated with different concentrations of Sodium Cyanide (NaCN) and then assayed for SOD activity. The level of significance was assessed using Student’s t-test. Results: The serum SOD level in tubercular subjects (both pulmonary and extra-pulmonary; with treatment started between 0-15 days) were significantly (p-value <0.01) elevated as compared to the control and lung-disease subjects. Significant decrease in serum SOD levels was observed after one-month of A-TB drug treatment signifying a decrease in mycobacterial load in host tissues. The iron co-factored SOD secreted by M.tuberculosisis was found to be resistant to NaCN whereas Copper-Zinc (Cu-Zn) co-factored SOD was inhibited by NaCN. Conclusion: The serum SOD assay, used in the present study, differentiated between human and mycobacterial origin-SOD, on incubation with NaCN, and diagnosed pulmonary as well as extra-pulmonary TB cases with confidence. Thus, it can be used as a simple, rapid, inexpensive, yet highly sensitive and specific- assay for detection of both human and bovine tuberculosis and primary and secondary drug-resistant cases. anti-tuberculosis treatment diagnostic marker extra-pulmonary tuberculosis pulmonary tuberculosis sodium-cyanide Medicine R In Journal of Clinical and Diagnostic Research JCDR Research and Publications Private Limited, 2009 13(2019), 7, Seite BC01-BC03 (DE-627)789478048 (DE-600)2775283-5 0973709X nnns volume:13 year:2019 number:7 pages:BC01-BC03 https://doi.org/10.7860/JCDR/2019/35298.12968 kostenfrei https://doaj.org/article/4b68fa3cd6eb44f1968f01eb748993d6 kostenfrei https://jcdr.net/articles/PDF/12968/35298_PD(V-3_NG_KM)_CE[Ra1]_F(KM)_PF1(AJ_SHU)_PFA(AJ_SHU)_PN(SL).pdf kostenfrei https://doaj.org/toc/2249-782X Journal toc kostenfrei https://doaj.org/toc/0973-709X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2019 7 BC01-BC03 |
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10.7860/JCDR/2019/35298.12968 doi (DE-627)DOAJ076902382 (DE-599)DOAJ4b68fa3cd6eb44f1968f01eb748993d6 DE-627 ger DE-627 rakwb eng Dipak Kumar Chattopadhyay verfasserin aut Superoxide Dismutase: A Biomarker for Early Diagnosis of Tuberculosis 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: Tuberculosis (TB) is a major public health concern in developing countries. Traditional diagnostic methods such as microscopic detection of organism and histopathological examination are limited in their capacity to provide information on prognosis and treatment efficiency. Abundantly secreted extracellular Superoxide Dismutase (SOD) protects the Mycobacterium tuberculosis (MTB), and elevated levels of the biomolecule are found in tissues and fluids of tubercular patients. Aim: To estimate the levels of serum SOD in lung control disease and tubercular patients; assess the diagnostic potential of SOD assay for detection of tuberculosis at an early onset stage and quantify the serum SOD activity to monitor the course of infection and determine the effectiveness of the anti-Tubercular (anti-TB) drug treatment. Materials and Methods: The present observational study was conducted on 180 participants at B.S. Medical College and Hospital, Bankura, West Bengal, India. The participants were divided into three groups: Group 1: Normal Control (n=30), Group 2: Disease Control/Lung-Disease Control (n=27) and Group 3: Tubercular Subjects (3A- Pulmonary TB and 3B- Extrapulmonary TB) (n=76). Serum SOD levels of the participants were measured spectrophotometrically. The serum SOD levels of the patients were re-measured after one-month of A-TB drug treatment. In addition, to increase the specificity of the test, the serum of tubercular subjects was incubated with different concentrations of Sodium Cyanide (NaCN) and then assayed for SOD activity. The level of significance was assessed using Student’s t-test. Results: The serum SOD level in tubercular subjects (both pulmonary and extra-pulmonary; with treatment started between 0-15 days) were significantly (p-value <0.01) elevated as compared to the control and lung-disease subjects. Significant decrease in serum SOD levels was observed after one-month of A-TB drug treatment signifying a decrease in mycobacterial load in host tissues. The iron co-factored SOD secreted by M.tuberculosisis was found to be resistant to NaCN whereas Copper-Zinc (Cu-Zn) co-factored SOD was inhibited by NaCN. Conclusion: The serum SOD assay, used in the present study, differentiated between human and mycobacterial origin-SOD, on incubation with NaCN, and diagnosed pulmonary as well as extra-pulmonary TB cases with confidence. Thus, it can be used as a simple, rapid, inexpensive, yet highly sensitive and specific- assay for detection of both human and bovine tuberculosis and primary and secondary drug-resistant cases. anti-tuberculosis treatment diagnostic marker extra-pulmonary tuberculosis pulmonary tuberculosis sodium-cyanide Medicine R In Journal of Clinical and Diagnostic Research JCDR Research and Publications Private Limited, 2009 13(2019), 7, Seite BC01-BC03 (DE-627)789478048 (DE-600)2775283-5 0973709X nnns volume:13 year:2019 number:7 pages:BC01-BC03 https://doi.org/10.7860/JCDR/2019/35298.12968 kostenfrei https://doaj.org/article/4b68fa3cd6eb44f1968f01eb748993d6 kostenfrei https://jcdr.net/articles/PDF/12968/35298_PD(V-3_NG_KM)_CE[Ra1]_F(KM)_PF1(AJ_SHU)_PFA(AJ_SHU)_PN(SL).pdf kostenfrei https://doaj.org/toc/2249-782X Journal toc kostenfrei https://doaj.org/toc/0973-709X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2019 7 BC01-BC03 |
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10.7860/JCDR/2019/35298.12968 doi (DE-627)DOAJ076902382 (DE-599)DOAJ4b68fa3cd6eb44f1968f01eb748993d6 DE-627 ger DE-627 rakwb eng Dipak Kumar Chattopadhyay verfasserin aut Superoxide Dismutase: A Biomarker for Early Diagnosis of Tuberculosis 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: Tuberculosis (TB) is a major public health concern in developing countries. Traditional diagnostic methods such as microscopic detection of organism and histopathological examination are limited in their capacity to provide information on prognosis and treatment efficiency. Abundantly secreted extracellular Superoxide Dismutase (SOD) protects the Mycobacterium tuberculosis (MTB), and elevated levels of the biomolecule are found in tissues and fluids of tubercular patients. Aim: To estimate the levels of serum SOD in lung control disease and tubercular patients; assess the diagnostic potential of SOD assay for detection of tuberculosis at an early onset stage and quantify the serum SOD activity to monitor the course of infection and determine the effectiveness of the anti-Tubercular (anti-TB) drug treatment. Materials and Methods: The present observational study was conducted on 180 participants at B.S. Medical College and Hospital, Bankura, West Bengal, India. The participants were divided into three groups: Group 1: Normal Control (n=30), Group 2: Disease Control/Lung-Disease Control (n=27) and Group 3: Tubercular Subjects (3A- Pulmonary TB and 3B- Extrapulmonary TB) (n=76). Serum SOD levels of the participants were measured spectrophotometrically. The serum SOD levels of the patients were re-measured after one-month of A-TB drug treatment. In addition, to increase the specificity of the test, the serum of tubercular subjects was incubated with different concentrations of Sodium Cyanide (NaCN) and then assayed for SOD activity. The level of significance was assessed using Student’s t-test. Results: The serum SOD level in tubercular subjects (both pulmonary and extra-pulmonary; with treatment started between 0-15 days) were significantly (p-value <0.01) elevated as compared to the control and lung-disease subjects. Significant decrease in serum SOD levels was observed after one-month of A-TB drug treatment signifying a decrease in mycobacterial load in host tissues. The iron co-factored SOD secreted by M.tuberculosisis was found to be resistant to NaCN whereas Copper-Zinc (Cu-Zn) co-factored SOD was inhibited by NaCN. Conclusion: The serum SOD assay, used in the present study, differentiated between human and mycobacterial origin-SOD, on incubation with NaCN, and diagnosed pulmonary as well as extra-pulmonary TB cases with confidence. Thus, it can be used as a simple, rapid, inexpensive, yet highly sensitive and specific- assay for detection of both human and bovine tuberculosis and primary and secondary drug-resistant cases. anti-tuberculosis treatment diagnostic marker extra-pulmonary tuberculosis pulmonary tuberculosis sodium-cyanide Medicine R In Journal of Clinical and Diagnostic Research JCDR Research and Publications Private Limited, 2009 13(2019), 7, Seite BC01-BC03 (DE-627)789478048 (DE-600)2775283-5 0973709X nnns volume:13 year:2019 number:7 pages:BC01-BC03 https://doi.org/10.7860/JCDR/2019/35298.12968 kostenfrei https://doaj.org/article/4b68fa3cd6eb44f1968f01eb748993d6 kostenfrei https://jcdr.net/articles/PDF/12968/35298_PD(V-3_NG_KM)_CE[Ra1]_F(KM)_PF1(AJ_SHU)_PFA(AJ_SHU)_PN(SL).pdf kostenfrei https://doaj.org/toc/2249-782X Journal toc kostenfrei https://doaj.org/toc/0973-709X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2019 7 BC01-BC03 |
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Superoxide Dismutase: A Biomarker for Early Diagnosis of Tuberculosis |
| abstract |
Introduction: Tuberculosis (TB) is a major public health concern in developing countries. Traditional diagnostic methods such as microscopic detection of organism and histopathological examination are limited in their capacity to provide information on prognosis and treatment efficiency. Abundantly secreted extracellular Superoxide Dismutase (SOD) protects the Mycobacterium tuberculosis (MTB), and elevated levels of the biomolecule are found in tissues and fluids of tubercular patients. Aim: To estimate the levels of serum SOD in lung control disease and tubercular patients; assess the diagnostic potential of SOD assay for detection of tuberculosis at an early onset stage and quantify the serum SOD activity to monitor the course of infection and determine the effectiveness of the anti-Tubercular (anti-TB) drug treatment. Materials and Methods: The present observational study was conducted on 180 participants at B.S. Medical College and Hospital, Bankura, West Bengal, India. The participants were divided into three groups: Group 1: Normal Control (n=30), Group 2: Disease Control/Lung-Disease Control (n=27) and Group 3: Tubercular Subjects (3A- Pulmonary TB and 3B- Extrapulmonary TB) (n=76). Serum SOD levels of the participants were measured spectrophotometrically. The serum SOD levels of the patients were re-measured after one-month of A-TB drug treatment. In addition, to increase the specificity of the test, the serum of tubercular subjects was incubated with different concentrations of Sodium Cyanide (NaCN) and then assayed for SOD activity. The level of significance was assessed using Student’s t-test. Results: The serum SOD level in tubercular subjects (both pulmonary and extra-pulmonary; with treatment started between 0-15 days) were significantly (p-value <0.01) elevated as compared to the control and lung-disease subjects. Significant decrease in serum SOD levels was observed after one-month of A-TB drug treatment signifying a decrease in mycobacterial load in host tissues. The iron co-factored SOD secreted by M.tuberculosisis was found to be resistant to NaCN whereas Copper-Zinc (Cu-Zn) co-factored SOD was inhibited by NaCN. Conclusion: The serum SOD assay, used in the present study, differentiated between human and mycobacterial origin-SOD, on incubation with NaCN, and diagnosed pulmonary as well as extra-pulmonary TB cases with confidence. Thus, it can be used as a simple, rapid, inexpensive, yet highly sensitive and specific- assay for detection of both human and bovine tuberculosis and primary and secondary drug-resistant cases. |
| abstractGer |
Introduction: Tuberculosis (TB) is a major public health concern in developing countries. Traditional diagnostic methods such as microscopic detection of organism and histopathological examination are limited in their capacity to provide information on prognosis and treatment efficiency. Abundantly secreted extracellular Superoxide Dismutase (SOD) protects the Mycobacterium tuberculosis (MTB), and elevated levels of the biomolecule are found in tissues and fluids of tubercular patients. Aim: To estimate the levels of serum SOD in lung control disease and tubercular patients; assess the diagnostic potential of SOD assay for detection of tuberculosis at an early onset stage and quantify the serum SOD activity to monitor the course of infection and determine the effectiveness of the anti-Tubercular (anti-TB) drug treatment. Materials and Methods: The present observational study was conducted on 180 participants at B.S. Medical College and Hospital, Bankura, West Bengal, India. The participants were divided into three groups: Group 1: Normal Control (n=30), Group 2: Disease Control/Lung-Disease Control (n=27) and Group 3: Tubercular Subjects (3A- Pulmonary TB and 3B- Extrapulmonary TB) (n=76). Serum SOD levels of the participants were measured spectrophotometrically. The serum SOD levels of the patients were re-measured after one-month of A-TB drug treatment. In addition, to increase the specificity of the test, the serum of tubercular subjects was incubated with different concentrations of Sodium Cyanide (NaCN) and then assayed for SOD activity. The level of significance was assessed using Student’s t-test. Results: The serum SOD level in tubercular subjects (both pulmonary and extra-pulmonary; with treatment started between 0-15 days) were significantly (p-value <0.01) elevated as compared to the control and lung-disease subjects. Significant decrease in serum SOD levels was observed after one-month of A-TB drug treatment signifying a decrease in mycobacterial load in host tissues. The iron co-factored SOD secreted by M.tuberculosisis was found to be resistant to NaCN whereas Copper-Zinc (Cu-Zn) co-factored SOD was inhibited by NaCN. Conclusion: The serum SOD assay, used in the present study, differentiated between human and mycobacterial origin-SOD, on incubation with NaCN, and diagnosed pulmonary as well as extra-pulmonary TB cases with confidence. Thus, it can be used as a simple, rapid, inexpensive, yet highly sensitive and specific- assay for detection of both human and bovine tuberculosis and primary and secondary drug-resistant cases. |
| abstract_unstemmed |
Introduction: Tuberculosis (TB) is a major public health concern in developing countries. Traditional diagnostic methods such as microscopic detection of organism and histopathological examination are limited in their capacity to provide information on prognosis and treatment efficiency. Abundantly secreted extracellular Superoxide Dismutase (SOD) protects the Mycobacterium tuberculosis (MTB), and elevated levels of the biomolecule are found in tissues and fluids of tubercular patients. Aim: To estimate the levels of serum SOD in lung control disease and tubercular patients; assess the diagnostic potential of SOD assay for detection of tuberculosis at an early onset stage and quantify the serum SOD activity to monitor the course of infection and determine the effectiveness of the anti-Tubercular (anti-TB) drug treatment. Materials and Methods: The present observational study was conducted on 180 participants at B.S. Medical College and Hospital, Bankura, West Bengal, India. The participants were divided into three groups: Group 1: Normal Control (n=30), Group 2: Disease Control/Lung-Disease Control (n=27) and Group 3: Tubercular Subjects (3A- Pulmonary TB and 3B- Extrapulmonary TB) (n=76). Serum SOD levels of the participants were measured spectrophotometrically. The serum SOD levels of the patients were re-measured after one-month of A-TB drug treatment. In addition, to increase the specificity of the test, the serum of tubercular subjects was incubated with different concentrations of Sodium Cyanide (NaCN) and then assayed for SOD activity. The level of significance was assessed using Student’s t-test. Results: The serum SOD level in tubercular subjects (both pulmonary and extra-pulmonary; with treatment started between 0-15 days) were significantly (p-value <0.01) elevated as compared to the control and lung-disease subjects. Significant decrease in serum SOD levels was observed after one-month of A-TB drug treatment signifying a decrease in mycobacterial load in host tissues. The iron co-factored SOD secreted by M.tuberculosisis was found to be resistant to NaCN whereas Copper-Zinc (Cu-Zn) co-factored SOD was inhibited by NaCN. Conclusion: The serum SOD assay, used in the present study, differentiated between human and mycobacterial origin-SOD, on incubation with NaCN, and diagnosed pulmonary as well as extra-pulmonary TB cases with confidence. Thus, it can be used as a simple, rapid, inexpensive, yet highly sensitive and specific- assay for detection of both human and bovine tuberculosis and primary and secondary drug-resistant cases. |
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Superoxide Dismutase: A Biomarker for Early Diagnosis of Tuberculosis |
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https://doi.org/10.7860/JCDR/2019/35298.12968 https://doaj.org/article/4b68fa3cd6eb44f1968f01eb748993d6 https://jcdr.net/articles/PDF/12968/35298_PD(V-3_NG_KM)_CE[Ra1]_F(KM)_PF1(AJ_SHU)_PFA(AJ_SHU)_PN(SL).pdf https://doaj.org/toc/2249-782X https://doaj.org/toc/0973-709X |
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