Cytoglobin expression in oxidative stressed liver during systemic chronic normobaric hypoxia and relation with HIF-1α

<p<<strong<Background:</strong< Liver is sensitive against hypoxia and hypoxia will stabilize HIF-1α. At the same time, hypoxia will produce reactive oxygen species (ROS) which can be scavenged by Cygb. The purpose of our study is to know, if normobaric hypoxia can induce Cygb expr...
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Autor*in:	Sri W.A. Jusman [verfasserIn] Febriana C. Iswanti [verfasserIn] Franciscus D. Suyatna [verfasserIn] Frans Ferdinal [verfasserIn] Septelia I. Wanandi [verfasserIn] Mohamad Sadikin [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2014

Schlagwörter:	cytoglobin HIF-1α liver oxidative stress systemic chronic normobaric hypoxia

Übergeordnetes Werk:	In: Medical Journal of Indonesia - Faculty of Medicine Universitas Indonesia, 2013, 23(2014), 3, Seite 133-8
Übergeordnetes Werk:	volume:23 ; year:2014 ; number:3 ; pages:133-8

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.13181/mji.v23i3.1025

Katalog-ID:	DOAJ077025970

Internformat


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245	1	0	\|a Cytoglobin expression in oxidative stressed liver during systemic chronic normobaric hypoxia and relation with HIF-1α
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520			\|a <p<<strong<Background:</strong< Liver is sensitive against hypoxia and hypoxia will stabilize HIF-1α. At the same time, hypoxia will produce reactive oxygen species (ROS) which can be scavenged by Cygb. The purpose of our study is to know, if normobaric hypoxia can induce Cygb expression and its association with HIF-1α stabilization.</p<<p<<strong<Methods:</strong< This is an experimental study using 28 male Sprague-Dawley rats, 150-200 g weight. Rats are divided into 7 groups: control group and treatment groups that are kept in hypoxic chamber (10% O<sub<2</sub<: 90% N<sub<2</sub<) for 6 hours, 1, 2, 3, 7 and 14 days. All rats are euthanized after treatment and liver tissue are isolated, homogenized and analyzed for oxidative stress parameter, expression of Cygb and HIF-1α.</p<<p<<strong<Results:</strong< Expression of Cygb mRNA and protein was increased on the day-1 after treatment and reach the maximum expression on the day-2 of hypoxia treatment. But, the expression was decreased after the day-3 and slightly increased at the day-14 of hypoxia. The correlation between expression of Cygb and oxidative stress parameter was strongly correlated. Cygb mRNA, as well as protein, showed the same kinetic as the HIF-1, all increased about day-1 and day-2.</p<<div<<strong<Conclusion:</strong< Systemic chronic hypoxia and/or oxidative stress up-regulated HIF-1α mRNA which is correlated with the Cygb mRNA and protein expression. Cygb mRNA as well as Cygb protein showed the same kinetic as the HIF-1, all increased about day-1 and day-2 suggesting that Cygb could be under the regulation of HIF-1, but could be controlled also by other factor than HIF-1.</div<
650		4	\|a cytoglobin
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650		4	\|a systemic chronic normobaric hypoxia
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700	0		\|a Febriana C. Iswanti \|e verfasserin \|4 aut
700	0		\|a Franciscus D. Suyatna \|e verfasserin \|4 aut
700	0		\|a Frans Ferdinal \|e verfasserin \|4 aut
700	0		\|a Septelia I. Wanandi \|e verfasserin \|4 aut
700	0		\|a Mohamad Sadikin \|e verfasserin \|4 aut
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allfields	10.13181/mji.v23i3.1025 doi (DE-627)DOAJ077025970 (DE-599)DOAJ3df887e8bb4c4e5393ede7e525ddc0ea DE-627 ger DE-627 rakwb eng R5-920 Sri W.A. Jusman verfasserin aut Cytoglobin expression in oxidative stressed liver during systemic chronic normobaric hypoxia and relation with HIF-1α 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<<strong<Background:</strong< Liver is sensitive against hypoxia and hypoxia will stabilize HIF-1α. At the same time, hypoxia will produce reactive oxygen species (ROS) which can be scavenged by Cygb. The purpose of our study is to know, if normobaric hypoxia can induce Cygb expression and its association with HIF-1α stabilization.</p<<p<<strong<Methods:</strong< This is an experimental study using 28 male Sprague-Dawley rats, 150-200 g weight. Rats are divided into 7 groups: control group and treatment groups that are kept in hypoxic chamber (10% O<sub<2</sub<: 90% N<sub<2</sub<) for 6 hours, 1, 2, 3, 7 and 14 days. All rats are euthanized after treatment and liver tissue are isolated, homogenized and analyzed for oxidative stress parameter, expression of Cygb and HIF-1α.</p<<p<<strong<Results:</strong< Expression of Cygb mRNA and protein was increased on the day-1 after treatment and reach the maximum expression on the day-2 of hypoxia treatment. But, the expression was decreased after the day-3 and slightly increased at the day-14 of hypoxia. The correlation between expression of Cygb and oxidative stress parameter was strongly correlated. Cygb mRNA, as well as protein, showed the same kinetic as the HIF-1, all increased about day-1 and day-2.</p<<div<<strong<Conclusion:</strong< Systemic chronic hypoxia and/or oxidative stress up-regulated HIF-1α mRNA which is correlated with the Cygb mRNA and protein expression. Cygb mRNA as well as Cygb protein showed the same kinetic as the HIF-1, all increased about day-1 and day-2 suggesting that Cygb could be under the regulation of HIF-1, but could be controlled also by other factor than HIF-1.</div< cytoglobin HIF-1α liver oxidative stress systemic chronic normobaric hypoxia Medicine (General) Febriana C. Iswanti verfasserin aut Franciscus D. Suyatna verfasserin aut Frans Ferdinal verfasserin aut Septelia I. Wanandi verfasserin aut Mohamad Sadikin verfasserin aut In Medical Journal of Indonesia Faculty of Medicine Universitas Indonesia, 2013 23(2014), 3, Seite 133-8 (DE-627)747137862 (DE-600)2716886-4 22528083 nnns volume:23 year:2014 number:3 pages:133-8 https://doi.org/10.13181/mji.v23i3.1025 kostenfrei https://doaj.org/article/3df887e8bb4c4e5393ede7e525ddc0ea kostenfrei http://mji.ui.ac.id/journal/index.php/mji/article/view/1025 kostenfrei https://doaj.org/toc/0853-1773 Journal toc kostenfrei https://doaj.org/toc/2252-8083 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2014 3 133-8
spelling	10.13181/mji.v23i3.1025 doi (DE-627)DOAJ077025970 (DE-599)DOAJ3df887e8bb4c4e5393ede7e525ddc0ea DE-627 ger DE-627 rakwb eng R5-920 Sri W.A. Jusman verfasserin aut Cytoglobin expression in oxidative stressed liver during systemic chronic normobaric hypoxia and relation with HIF-1α 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<<strong<Background:</strong< Liver is sensitive against hypoxia and hypoxia will stabilize HIF-1α. At the same time, hypoxia will produce reactive oxygen species (ROS) which can be scavenged by Cygb. The purpose of our study is to know, if normobaric hypoxia can induce Cygb expression and its association with HIF-1α stabilization.</p<<p<<strong<Methods:</strong< This is an experimental study using 28 male Sprague-Dawley rats, 150-200 g weight. Rats are divided into 7 groups: control group and treatment groups that are kept in hypoxic chamber (10% O<sub<2</sub<: 90% N<sub<2</sub<) for 6 hours, 1, 2, 3, 7 and 14 days. All rats are euthanized after treatment and liver tissue are isolated, homogenized and analyzed for oxidative stress parameter, expression of Cygb and HIF-1α.</p<<p<<strong<Results:</strong< Expression of Cygb mRNA and protein was increased on the day-1 after treatment and reach the maximum expression on the day-2 of hypoxia treatment. But, the expression was decreased after the day-3 and slightly increased at the day-14 of hypoxia. The correlation between expression of Cygb and oxidative stress parameter was strongly correlated. Cygb mRNA, as well as protein, showed the same kinetic as the HIF-1, all increased about day-1 and day-2.</p<<div<<strong<Conclusion:</strong< Systemic chronic hypoxia and/or oxidative stress up-regulated HIF-1α mRNA which is correlated with the Cygb mRNA and protein expression. Cygb mRNA as well as Cygb protein showed the same kinetic as the HIF-1, all increased about day-1 and day-2 suggesting that Cygb could be under the regulation of HIF-1, but could be controlled also by other factor than HIF-1.</div< cytoglobin HIF-1α liver oxidative stress systemic chronic normobaric hypoxia Medicine (General) Febriana C. Iswanti verfasserin aut Franciscus D. Suyatna verfasserin aut Frans Ferdinal verfasserin aut Septelia I. Wanandi verfasserin aut Mohamad Sadikin verfasserin aut In Medical Journal of Indonesia Faculty of Medicine Universitas Indonesia, 2013 23(2014), 3, Seite 133-8 (DE-627)747137862 (DE-600)2716886-4 22528083 nnns volume:23 year:2014 number:3 pages:133-8 https://doi.org/10.13181/mji.v23i3.1025 kostenfrei https://doaj.org/article/3df887e8bb4c4e5393ede7e525ddc0ea kostenfrei http://mji.ui.ac.id/journal/index.php/mji/article/view/1025 kostenfrei https://doaj.org/toc/0853-1773 Journal toc kostenfrei https://doaj.org/toc/2252-8083 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2014 3 133-8
allfields_unstemmed	10.13181/mji.v23i3.1025 doi (DE-627)DOAJ077025970 (DE-599)DOAJ3df887e8bb4c4e5393ede7e525ddc0ea DE-627 ger DE-627 rakwb eng R5-920 Sri W.A. Jusman verfasserin aut Cytoglobin expression in oxidative stressed liver during systemic chronic normobaric hypoxia and relation with HIF-1α 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<<strong<Background:</strong< Liver is sensitive against hypoxia and hypoxia will stabilize HIF-1α. At the same time, hypoxia will produce reactive oxygen species (ROS) which can be scavenged by Cygb. The purpose of our study is to know, if normobaric hypoxia can induce Cygb expression and its association with HIF-1α stabilization.</p<<p<<strong<Methods:</strong< This is an experimental study using 28 male Sprague-Dawley rats, 150-200 g weight. Rats are divided into 7 groups: control group and treatment groups that are kept in hypoxic chamber (10% O<sub<2</sub<: 90% N<sub<2</sub<) for 6 hours, 1, 2, 3, 7 and 14 days. All rats are euthanized after treatment and liver tissue are isolated, homogenized and analyzed for oxidative stress parameter, expression of Cygb and HIF-1α.</p<<p<<strong<Results:</strong< Expression of Cygb mRNA and protein was increased on the day-1 after treatment and reach the maximum expression on the day-2 of hypoxia treatment. But, the expression was decreased after the day-3 and slightly increased at the day-14 of hypoxia. The correlation between expression of Cygb and oxidative stress parameter was strongly correlated. Cygb mRNA, as well as protein, showed the same kinetic as the HIF-1, all increased about day-1 and day-2.</p<<div<<strong<Conclusion:</strong< Systemic chronic hypoxia and/or oxidative stress up-regulated HIF-1α mRNA which is correlated with the Cygb mRNA and protein expression. Cygb mRNA as well as Cygb protein showed the same kinetic as the HIF-1, all increased about day-1 and day-2 suggesting that Cygb could be under the regulation of HIF-1, but could be controlled also by other factor than HIF-1.</div< cytoglobin HIF-1α liver oxidative stress systemic chronic normobaric hypoxia Medicine (General) Febriana C. Iswanti verfasserin aut Franciscus D. Suyatna verfasserin aut Frans Ferdinal verfasserin aut Septelia I. Wanandi verfasserin aut Mohamad Sadikin verfasserin aut In Medical Journal of Indonesia Faculty of Medicine Universitas Indonesia, 2013 23(2014), 3, Seite 133-8 (DE-627)747137862 (DE-600)2716886-4 22528083 nnns volume:23 year:2014 number:3 pages:133-8 https://doi.org/10.13181/mji.v23i3.1025 kostenfrei https://doaj.org/article/3df887e8bb4c4e5393ede7e525ddc0ea kostenfrei http://mji.ui.ac.id/journal/index.php/mji/article/view/1025 kostenfrei https://doaj.org/toc/0853-1773 Journal toc kostenfrei https://doaj.org/toc/2252-8083 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2014 3 133-8
allfieldsGer	10.13181/mji.v23i3.1025 doi (DE-627)DOAJ077025970 (DE-599)DOAJ3df887e8bb4c4e5393ede7e525ddc0ea DE-627 ger DE-627 rakwb eng R5-920 Sri W.A. Jusman verfasserin aut Cytoglobin expression in oxidative stressed liver during systemic chronic normobaric hypoxia and relation with HIF-1α 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<<strong<Background:</strong< Liver is sensitive against hypoxia and hypoxia will stabilize HIF-1α. At the same time, hypoxia will produce reactive oxygen species (ROS) which can be scavenged by Cygb. The purpose of our study is to know, if normobaric hypoxia can induce Cygb expression and its association with HIF-1α stabilization.</p<<p<<strong<Methods:</strong< This is an experimental study using 28 male Sprague-Dawley rats, 150-200 g weight. Rats are divided into 7 groups: control group and treatment groups that are kept in hypoxic chamber (10% O<sub<2</sub<: 90% N<sub<2</sub<) for 6 hours, 1, 2, 3, 7 and 14 days. All rats are euthanized after treatment and liver tissue are isolated, homogenized and analyzed for oxidative stress parameter, expression of Cygb and HIF-1α.</p<<p<<strong<Results:</strong< Expression of Cygb mRNA and protein was increased on the day-1 after treatment and reach the maximum expression on the day-2 of hypoxia treatment. But, the expression was decreased after the day-3 and slightly increased at the day-14 of hypoxia. The correlation between expression of Cygb and oxidative stress parameter was strongly correlated. Cygb mRNA, as well as protein, showed the same kinetic as the HIF-1, all increased about day-1 and day-2.</p<<div<<strong<Conclusion:</strong< Systemic chronic hypoxia and/or oxidative stress up-regulated HIF-1α mRNA which is correlated with the Cygb mRNA and protein expression. Cygb mRNA as well as Cygb protein showed the same kinetic as the HIF-1, all increased about day-1 and day-2 suggesting that Cygb could be under the regulation of HIF-1, but could be controlled also by other factor than HIF-1.</div< cytoglobin HIF-1α liver oxidative stress systemic chronic normobaric hypoxia Medicine (General) Febriana C. Iswanti verfasserin aut Franciscus D. Suyatna verfasserin aut Frans Ferdinal verfasserin aut Septelia I. Wanandi verfasserin aut Mohamad Sadikin verfasserin aut In Medical Journal of Indonesia Faculty of Medicine Universitas Indonesia, 2013 23(2014), 3, Seite 133-8 (DE-627)747137862 (DE-600)2716886-4 22528083 nnns volume:23 year:2014 number:3 pages:133-8 https://doi.org/10.13181/mji.v23i3.1025 kostenfrei https://doaj.org/article/3df887e8bb4c4e5393ede7e525ddc0ea kostenfrei http://mji.ui.ac.id/journal/index.php/mji/article/view/1025 kostenfrei https://doaj.org/toc/0853-1773 Journal toc kostenfrei https://doaj.org/toc/2252-8083 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2014 3 133-8
allfieldsSound	10.13181/mji.v23i3.1025 doi (DE-627)DOAJ077025970 (DE-599)DOAJ3df887e8bb4c4e5393ede7e525ddc0ea DE-627 ger DE-627 rakwb eng R5-920 Sri W.A. Jusman verfasserin aut Cytoglobin expression in oxidative stressed liver during systemic chronic normobaric hypoxia and relation with HIF-1α 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<<strong<Background:</strong< Liver is sensitive against hypoxia and hypoxia will stabilize HIF-1α. At the same time, hypoxia will produce reactive oxygen species (ROS) which can be scavenged by Cygb. The purpose of our study is to know, if normobaric hypoxia can induce Cygb expression and its association with HIF-1α stabilization.</p<<p<<strong<Methods:</strong< This is an experimental study using 28 male Sprague-Dawley rats, 150-200 g weight. Rats are divided into 7 groups: control group and treatment groups that are kept in hypoxic chamber (10% O<sub<2</sub<: 90% N<sub<2</sub<) for 6 hours, 1, 2, 3, 7 and 14 days. All rats are euthanized after treatment and liver tissue are isolated, homogenized and analyzed for oxidative stress parameter, expression of Cygb and HIF-1α.</p<<p<<strong<Results:</strong< Expression of Cygb mRNA and protein was increased on the day-1 after treatment and reach the maximum expression on the day-2 of hypoxia treatment. But, the expression was decreased after the day-3 and slightly increased at the day-14 of hypoxia. The correlation between expression of Cygb and oxidative stress parameter was strongly correlated. Cygb mRNA, as well as protein, showed the same kinetic as the HIF-1, all increased about day-1 and day-2.</p<<div<<strong<Conclusion:</strong< Systemic chronic hypoxia and/or oxidative stress up-regulated HIF-1α mRNA which is correlated with the Cygb mRNA and protein expression. Cygb mRNA as well as Cygb protein showed the same kinetic as the HIF-1, all increased about day-1 and day-2 suggesting that Cygb could be under the regulation of HIF-1, but could be controlled also by other factor than HIF-1.</div< cytoglobin HIF-1α liver oxidative stress systemic chronic normobaric hypoxia Medicine (General) Febriana C. Iswanti verfasserin aut Franciscus D. Suyatna verfasserin aut Frans Ferdinal verfasserin aut Septelia I. Wanandi verfasserin aut Mohamad Sadikin verfasserin aut In Medical Journal of Indonesia Faculty of Medicine Universitas Indonesia, 2013 23(2014), 3, Seite 133-8 (DE-627)747137862 (DE-600)2716886-4 22528083 nnns volume:23 year:2014 number:3 pages:133-8 https://doi.org/10.13181/mji.v23i3.1025 kostenfrei https://doaj.org/article/3df887e8bb4c4e5393ede7e525ddc0ea kostenfrei http://mji.ui.ac.id/journal/index.php/mji/article/view/1025 kostenfrei https://doaj.org/toc/0853-1773 Journal toc kostenfrei https://doaj.org/toc/2252-8083 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2014 3 133-8
language	English
source	In Medical Journal of Indonesia 23(2014), 3, Seite 133-8 volume:23 year:2014 number:3 pages:133-8
sourceStr	In Medical Journal of Indonesia 23(2014), 3, Seite 133-8 volume:23 year:2014 number:3 pages:133-8
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	cytoglobin HIF-1α liver oxidative stress systemic chronic normobaric hypoxia Medicine (General)
isfreeaccess_bool	true
container_title	Medical Journal of Indonesia
authorswithroles_txt_mv	Sri W.A. Jusman @@aut@@ Febriana C. Iswanti @@aut@@ Franciscus D. Suyatna @@aut@@ Frans Ferdinal @@aut@@ Septelia I. Wanandi @@aut@@ Mohamad Sadikin @@aut@@
publishDateDaySort_date	2014-01-01T00:00:00Z
hierarchy_top_id	747137862
id	DOAJ077025970
language_de	englisch
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callnumber-first	R - Medicine
author	Sri W.A. Jusman
spellingShingle	Sri W.A. Jusman misc R5-920 misc cytoglobin misc HIF-1α misc liver misc oxidative stress misc systemic chronic normobaric hypoxia misc Medicine (General) Cytoglobin expression in oxidative stressed liver during systemic chronic normobaric hypoxia and relation with HIF-1α
authorStr	Sri W.A. Jusman
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topic_title	R5-920 Cytoglobin expression in oxidative stressed liver during systemic chronic normobaric hypoxia and relation with HIF-1α cytoglobin HIF-1α liver oxidative stress systemic chronic normobaric hypoxia
topic	misc R5-920 misc cytoglobin misc HIF-1α misc liver misc oxidative stress misc systemic chronic normobaric hypoxia misc Medicine (General)
topic_unstemmed	misc R5-920 misc cytoglobin misc HIF-1α misc liver misc oxidative stress misc systemic chronic normobaric hypoxia misc Medicine (General)
topic_browse	misc R5-920 misc cytoglobin misc HIF-1α misc liver misc oxidative stress misc systemic chronic normobaric hypoxia misc Medicine (General)
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title	Cytoglobin expression in oxidative stressed liver during systemic chronic normobaric hypoxia and relation with HIF-1α
ctrlnum	(DE-627)DOAJ077025970 (DE-599)DOAJ3df887e8bb4c4e5393ede7e525ddc0ea
title_full	Cytoglobin expression in oxidative stressed liver during systemic chronic normobaric hypoxia and relation with HIF-1α
author_sort	Sri W.A. Jusman
journal	Medical Journal of Indonesia
journalStr	Medical Journal of Indonesia
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lang_code	eng
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container_start_page	133
author_browse	Sri W.A. Jusman Febriana C. Iswanti Franciscus D. Suyatna Frans Ferdinal Septelia I. Wanandi Mohamad Sadikin
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author-letter	Sri W.A. Jusman
doi_str_mv	10.13181/mji.v23i3.1025
author2-role	verfasserin
title_sort	cytoglobin expression in oxidative stressed liver during systemic chronic normobaric hypoxia and relation with hif-1α
callnumber	R5-920
title_auth	Cytoglobin expression in oxidative stressed liver during systemic chronic normobaric hypoxia and relation with HIF-1α
abstract	<p<<strong<Background:</strong< Liver is sensitive against hypoxia and hypoxia will stabilize HIF-1α. At the same time, hypoxia will produce reactive oxygen species (ROS) which can be scavenged by Cygb. The purpose of our study is to know, if normobaric hypoxia can induce Cygb expression and its association with HIF-1α stabilization.</p<<p<<strong<Methods:</strong< This is an experimental study using 28 male Sprague-Dawley rats, 150-200 g weight. Rats are divided into 7 groups: control group and treatment groups that are kept in hypoxic chamber (10% O<sub<2</sub<: 90% N<sub<2</sub<) for 6 hours, 1, 2, 3, 7 and 14 days. All rats are euthanized after treatment and liver tissue are isolated, homogenized and analyzed for oxidative stress parameter, expression of Cygb and HIF-1α.</p<<p<<strong<Results:</strong< Expression of Cygb mRNA and protein was increased on the day-1 after treatment and reach the maximum expression on the day-2 of hypoxia treatment. But, the expression was decreased after the day-3 and slightly increased at the day-14 of hypoxia. The correlation between expression of Cygb and oxidative stress parameter was strongly correlated. Cygb mRNA, as well as protein, showed the same kinetic as the HIF-1, all increased about day-1 and day-2.</p<<div<<strong<Conclusion:</strong< Systemic chronic hypoxia and/or oxidative stress up-regulated HIF-1α mRNA which is correlated with the Cygb mRNA and protein expression. Cygb mRNA as well as Cygb protein showed the same kinetic as the HIF-1, all increased about day-1 and day-2 suggesting that Cygb could be under the regulation of HIF-1, but could be controlled also by other factor than HIF-1.</div<
abstractGer	<p<<strong<Background:</strong< Liver is sensitive against hypoxia and hypoxia will stabilize HIF-1α. At the same time, hypoxia will produce reactive oxygen species (ROS) which can be scavenged by Cygb. The purpose of our study is to know, if normobaric hypoxia can induce Cygb expression and its association with HIF-1α stabilization.</p<<p<<strong<Methods:</strong< This is an experimental study using 28 male Sprague-Dawley rats, 150-200 g weight. Rats are divided into 7 groups: control group and treatment groups that are kept in hypoxic chamber (10% O<sub<2</sub<: 90% N<sub<2</sub<) for 6 hours, 1, 2, 3, 7 and 14 days. All rats are euthanized after treatment and liver tissue are isolated, homogenized and analyzed for oxidative stress parameter, expression of Cygb and HIF-1α.</p<<p<<strong<Results:</strong< Expression of Cygb mRNA and protein was increased on the day-1 after treatment and reach the maximum expression on the day-2 of hypoxia treatment. But, the expression was decreased after the day-3 and slightly increased at the day-14 of hypoxia. The correlation between expression of Cygb and oxidative stress parameter was strongly correlated. Cygb mRNA, as well as protein, showed the same kinetic as the HIF-1, all increased about day-1 and day-2.</p<<div<<strong<Conclusion:</strong< Systemic chronic hypoxia and/or oxidative stress up-regulated HIF-1α mRNA which is correlated with the Cygb mRNA and protein expression. Cygb mRNA as well as Cygb protein showed the same kinetic as the HIF-1, all increased about day-1 and day-2 suggesting that Cygb could be under the regulation of HIF-1, but could be controlled also by other factor than HIF-1.</div<
abstract_unstemmed	<p<<strong<Background:</strong< Liver is sensitive against hypoxia and hypoxia will stabilize HIF-1α. At the same time, hypoxia will produce reactive oxygen species (ROS) which can be scavenged by Cygb. The purpose of our study is to know, if normobaric hypoxia can induce Cygb expression and its association with HIF-1α stabilization.</p<<p<<strong<Methods:</strong< This is an experimental study using 28 male Sprague-Dawley rats, 150-200 g weight. Rats are divided into 7 groups: control group and treatment groups that are kept in hypoxic chamber (10% O<sub<2</sub<: 90% N<sub<2</sub<) for 6 hours, 1, 2, 3, 7 and 14 days. All rats are euthanized after treatment and liver tissue are isolated, homogenized and analyzed for oxidative stress parameter, expression of Cygb and HIF-1α.</p<<p<<strong<Results:</strong< Expression of Cygb mRNA and protein was increased on the day-1 after treatment and reach the maximum expression on the day-2 of hypoxia treatment. But, the expression was decreased after the day-3 and slightly increased at the day-14 of hypoxia. The correlation between expression of Cygb and oxidative stress parameter was strongly correlated. Cygb mRNA, as well as protein, showed the same kinetic as the HIF-1, all increased about day-1 and day-2.</p<<div<<strong<Conclusion:</strong< Systemic chronic hypoxia and/or oxidative stress up-regulated HIF-1α mRNA which is correlated with the Cygb mRNA and protein expression. Cygb mRNA as well as Cygb protein showed the same kinetic as the HIF-1, all increased about day-1 and day-2 suggesting that Cygb could be under the regulation of HIF-1, but could be controlled also by other factor than HIF-1.</div<
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container_issue	3
title_short	Cytoglobin expression in oxidative stressed liver during systemic chronic normobaric hypoxia and relation with HIF-1α
url	https://doi.org/10.13181/mji.v23i3.1025 https://doaj.org/article/3df887e8bb4c4e5393ede7e525ddc0ea http://mji.ui.ac.id/journal/index.php/mji/article/view/1025 https://doaj.org/toc/0853-1773 https://doaj.org/toc/2252-8083
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author2	Febriana C. Iswanti Franciscus D. Suyatna Frans Ferdinal Septelia I. Wanandi Mohamad Sadikin
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up_date	2024-07-03T23:34:34.111Z
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