Adaption to glucose limitation is modulated by the pleotropic regulator CcpA, independent of selection pressure strength
Abstract Background A central theme in (micro)biology is understanding the molecular basis of fitness i.e. which strategies are successful under which conditions; how do organisms implement such strategies at the molecular level; and which constraints shape the trade-offs between alternative strateg...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Claire E. Price [verfasserIn] Filipe Branco dos Santos [verfasserIn] Anne Hesseling [verfasserIn] Jaakko J. Uusitalo [verfasserIn] Herwig Bachmann [verfasserIn] Vera Benavente [verfasserIn] Anisha Goel [verfasserIn] Jan Berkhout [verfasserIn] Frank J. Bruggeman [verfasserIn] Siewert-Jan Marrink [verfasserIn] Manolo Montalban-Lopez [verfasserIn] Anne de Jong [verfasserIn] Jan Kok [verfasserIn] Douwe Molenaar [verfasserIn] Bert Poolman [verfasserIn] Bas Teusink [verfasserIn] Oscar P. Kuipers [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2019 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
In: BMC Evolutionary Biology - BMC, 2003, 19(2019), 1, Seite 15 |
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| Übergeordnetes Werk: |
volume:19 ; year:2019 ; number:1 ; pages:15 |
| Links: |
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| DOI / URN: |
10.1186/s12862-018-1331-x |
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| Katalog-ID: |
DOAJ077162498 |
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| 520 | |a Abstract Background A central theme in (micro)biology is understanding the molecular basis of fitness i.e. which strategies are successful under which conditions; how do organisms implement such strategies at the molecular level; and which constraints shape the trade-offs between alternative strategies. Highly standardized microbial laboratory evolution experiments are ideally suited to approach these questions. For example, prolonged chemostats provide a constant environment in which the growth rate can be set, and the adaptive process of the organism to such environment can be subsequently characterized. Results We performed parallel laboratory evolution of Lactococcus lactis in chemostats varying the quantitative value of the selective pressure by imposing two different growth rates. A mutation in one specific amino acid residue of the global transcriptional regulator of carbon metabolism, CcpA, was selected in all of the evolution experiments performed. We subsequently showed that this mutation confers predictable fitness improvements at other glucose-limited growth rates as well. In silico protein structural analysis of wild type and evolved CcpA, as well as biochemical and phenotypic assays, provided the underpinning molecular mechanisms that resulted in the specific reprogramming favored in constant environments. Conclusion This study provides a comprehensive understanding of a case of microbial evolution and hints at the wide dynamic range that a single fitness-enhancing mutation may display. It demonstrates how the modulation of a pleiotropic regulator can be used by cells to improve one trait while simultaneously work around other limiting constraints, by fine-tuning the expression of a wide range of cellular processes. | ||
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| 650 | 4 | |a Systems biology | |
| 650 | 4 | |a Lactic acid bacteria | |
| 700 | 0 | |a Filipe Branco dos Santos |e verfasserin |4 aut | |
| 700 | 0 | |a Anne Hesseling |e verfasserin |4 aut | |
| 700 | 0 | |a Jaakko J. Uusitalo |e verfasserin |4 aut | |
| 700 | 0 | |a Herwig Bachmann |e verfasserin |4 aut | |
| 700 | 0 | |a Vera Benavente |e verfasserin |4 aut | |
| 700 | 0 | |a Anisha Goel |e verfasserin |4 aut | |
| 700 | 0 | |a Jan Berkhout |e verfasserin |4 aut | |
| 700 | 0 | |a Frank J. Bruggeman |e verfasserin |4 aut | |
| 700 | 0 | |a Siewert-Jan Marrink |e verfasserin |4 aut | |
| 700 | 0 | |a Manolo Montalban-Lopez |e verfasserin |4 aut | |
| 700 | 0 | |a Anne de Jong |e verfasserin |4 aut | |
| 700 | 0 | |a Jan Kok |e verfasserin |4 aut | |
| 700 | 0 | |a Douwe Molenaar |e verfasserin |4 aut | |
| 700 | 0 | |a Bert Poolman |e verfasserin |4 aut | |
| 700 | 0 | |a Bas Teusink |e verfasserin |4 aut | |
| 700 | 0 | |a Oscar P. Kuipers |e verfasserin |4 aut | |
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10.1186/s12862-018-1331-x doi (DE-627)DOAJ077162498 (DE-599)DOAJ268f290335c04c76aeea5bce0f5b920b DE-627 ger DE-627 rakwb eng QH359-425 Claire E. Price verfasserin aut Adaption to glucose limitation is modulated by the pleotropic regulator CcpA, independent of selection pressure strength 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background A central theme in (micro)biology is understanding the molecular basis of fitness i.e. which strategies are successful under which conditions; how do organisms implement such strategies at the molecular level; and which constraints shape the trade-offs between alternative strategies. Highly standardized microbial laboratory evolution experiments are ideally suited to approach these questions. For example, prolonged chemostats provide a constant environment in which the growth rate can be set, and the adaptive process of the organism to such environment can be subsequently characterized. Results We performed parallel laboratory evolution of Lactococcus lactis in chemostats varying the quantitative value of the selective pressure by imposing two different growth rates. A mutation in one specific amino acid residue of the global transcriptional regulator of carbon metabolism, CcpA, was selected in all of the evolution experiments performed. We subsequently showed that this mutation confers predictable fitness improvements at other glucose-limited growth rates as well. In silico protein structural analysis of wild type and evolved CcpA, as well as biochemical and phenotypic assays, provided the underpinning molecular mechanisms that resulted in the specific reprogramming favored in constant environments. Conclusion This study provides a comprehensive understanding of a case of microbial evolution and hints at the wide dynamic range that a single fitness-enhancing mutation may display. It demonstrates how the modulation of a pleiotropic regulator can be used by cells to improve one trait while simultaneously work around other limiting constraints, by fine-tuning the expression of a wide range of cellular processes. Evolution Systems biology Lactic acid bacteria Filipe Branco dos Santos verfasserin aut Anne Hesseling verfasserin aut Jaakko J. Uusitalo verfasserin aut Herwig Bachmann verfasserin aut Vera Benavente verfasserin aut Anisha Goel verfasserin aut Jan Berkhout verfasserin aut Frank J. Bruggeman verfasserin aut Siewert-Jan Marrink verfasserin aut Manolo Montalban-Lopez verfasserin aut Anne de Jong verfasserin aut Jan Kok verfasserin aut Douwe Molenaar verfasserin aut Bert Poolman verfasserin aut Bas Teusink verfasserin aut Oscar P. Kuipers verfasserin aut In BMC Evolutionary Biology BMC, 2003 19(2019), 1, Seite 15 (DE-627)32664489X (DE-600)2041493-6 14712148 nnns volume:19 year:2019 number:1 pages:15 https://doi.org/10.1186/s12862-018-1331-x kostenfrei https://doaj.org/article/268f290335c04c76aeea5bce0f5b920b kostenfrei http://link.springer.com/article/10.1186/s12862-018-1331-x kostenfrei https://doaj.org/toc/1471-2148 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2019 1 15 |
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10.1186/s12862-018-1331-x doi (DE-627)DOAJ077162498 (DE-599)DOAJ268f290335c04c76aeea5bce0f5b920b DE-627 ger DE-627 rakwb eng QH359-425 Claire E. Price verfasserin aut Adaption to glucose limitation is modulated by the pleotropic regulator CcpA, independent of selection pressure strength 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background A central theme in (micro)biology is understanding the molecular basis of fitness i.e. which strategies are successful under which conditions; how do organisms implement such strategies at the molecular level; and which constraints shape the trade-offs between alternative strategies. Highly standardized microbial laboratory evolution experiments are ideally suited to approach these questions. For example, prolonged chemostats provide a constant environment in which the growth rate can be set, and the adaptive process of the organism to such environment can be subsequently characterized. Results We performed parallel laboratory evolution of Lactococcus lactis in chemostats varying the quantitative value of the selective pressure by imposing two different growth rates. A mutation in one specific amino acid residue of the global transcriptional regulator of carbon metabolism, CcpA, was selected in all of the evolution experiments performed. We subsequently showed that this mutation confers predictable fitness improvements at other glucose-limited growth rates as well. In silico protein structural analysis of wild type and evolved CcpA, as well as biochemical and phenotypic assays, provided the underpinning molecular mechanisms that resulted in the specific reprogramming favored in constant environments. Conclusion This study provides a comprehensive understanding of a case of microbial evolution and hints at the wide dynamic range that a single fitness-enhancing mutation may display. It demonstrates how the modulation of a pleiotropic regulator can be used by cells to improve one trait while simultaneously work around other limiting constraints, by fine-tuning the expression of a wide range of cellular processes. Evolution Systems biology Lactic acid bacteria Filipe Branco dos Santos verfasserin aut Anne Hesseling verfasserin aut Jaakko J. Uusitalo verfasserin aut Herwig Bachmann verfasserin aut Vera Benavente verfasserin aut Anisha Goel verfasserin aut Jan Berkhout verfasserin aut Frank J. Bruggeman verfasserin aut Siewert-Jan Marrink verfasserin aut Manolo Montalban-Lopez verfasserin aut Anne de Jong verfasserin aut Jan Kok verfasserin aut Douwe Molenaar verfasserin aut Bert Poolman verfasserin aut Bas Teusink verfasserin aut Oscar P. Kuipers verfasserin aut In BMC Evolutionary Biology BMC, 2003 19(2019), 1, Seite 15 (DE-627)32664489X (DE-600)2041493-6 14712148 nnns volume:19 year:2019 number:1 pages:15 https://doi.org/10.1186/s12862-018-1331-x kostenfrei https://doaj.org/article/268f290335c04c76aeea5bce0f5b920b kostenfrei http://link.springer.com/article/10.1186/s12862-018-1331-x kostenfrei https://doaj.org/toc/1471-2148 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2019 1 15 |
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10.1186/s12862-018-1331-x doi (DE-627)DOAJ077162498 (DE-599)DOAJ268f290335c04c76aeea5bce0f5b920b DE-627 ger DE-627 rakwb eng QH359-425 Claire E. Price verfasserin aut Adaption to glucose limitation is modulated by the pleotropic regulator CcpA, independent of selection pressure strength 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background A central theme in (micro)biology is understanding the molecular basis of fitness i.e. which strategies are successful under which conditions; how do organisms implement such strategies at the molecular level; and which constraints shape the trade-offs between alternative strategies. Highly standardized microbial laboratory evolution experiments are ideally suited to approach these questions. For example, prolonged chemostats provide a constant environment in which the growth rate can be set, and the adaptive process of the organism to such environment can be subsequently characterized. Results We performed parallel laboratory evolution of Lactococcus lactis in chemostats varying the quantitative value of the selective pressure by imposing two different growth rates. A mutation in one specific amino acid residue of the global transcriptional regulator of carbon metabolism, CcpA, was selected in all of the evolution experiments performed. We subsequently showed that this mutation confers predictable fitness improvements at other glucose-limited growth rates as well. In silico protein structural analysis of wild type and evolved CcpA, as well as biochemical and phenotypic assays, provided the underpinning molecular mechanisms that resulted in the specific reprogramming favored in constant environments. Conclusion This study provides a comprehensive understanding of a case of microbial evolution and hints at the wide dynamic range that a single fitness-enhancing mutation may display. It demonstrates how the modulation of a pleiotropic regulator can be used by cells to improve one trait while simultaneously work around other limiting constraints, by fine-tuning the expression of a wide range of cellular processes. Evolution Systems biology Lactic acid bacteria Filipe Branco dos Santos verfasserin aut Anne Hesseling verfasserin aut Jaakko J. Uusitalo verfasserin aut Herwig Bachmann verfasserin aut Vera Benavente verfasserin aut Anisha Goel verfasserin aut Jan Berkhout verfasserin aut Frank J. Bruggeman verfasserin aut Siewert-Jan Marrink verfasserin aut Manolo Montalban-Lopez verfasserin aut Anne de Jong verfasserin aut Jan Kok verfasserin aut Douwe Molenaar verfasserin aut Bert Poolman verfasserin aut Bas Teusink verfasserin aut Oscar P. Kuipers verfasserin aut In BMC Evolutionary Biology BMC, 2003 19(2019), 1, Seite 15 (DE-627)32664489X (DE-600)2041493-6 14712148 nnns volume:19 year:2019 number:1 pages:15 https://doi.org/10.1186/s12862-018-1331-x kostenfrei https://doaj.org/article/268f290335c04c76aeea5bce0f5b920b kostenfrei http://link.springer.com/article/10.1186/s12862-018-1331-x kostenfrei https://doaj.org/toc/1471-2148 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2019 1 15 |
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10.1186/s12862-018-1331-x doi (DE-627)DOAJ077162498 (DE-599)DOAJ268f290335c04c76aeea5bce0f5b920b DE-627 ger DE-627 rakwb eng QH359-425 Claire E. Price verfasserin aut Adaption to glucose limitation is modulated by the pleotropic regulator CcpA, independent of selection pressure strength 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background A central theme in (micro)biology is understanding the molecular basis of fitness i.e. which strategies are successful under which conditions; how do organisms implement such strategies at the molecular level; and which constraints shape the trade-offs between alternative strategies. Highly standardized microbial laboratory evolution experiments are ideally suited to approach these questions. For example, prolonged chemostats provide a constant environment in which the growth rate can be set, and the adaptive process of the organism to such environment can be subsequently characterized. Results We performed parallel laboratory evolution of Lactococcus lactis in chemostats varying the quantitative value of the selective pressure by imposing two different growth rates. A mutation in one specific amino acid residue of the global transcriptional regulator of carbon metabolism, CcpA, was selected in all of the evolution experiments performed. We subsequently showed that this mutation confers predictable fitness improvements at other glucose-limited growth rates as well. In silico protein structural analysis of wild type and evolved CcpA, as well as biochemical and phenotypic assays, provided the underpinning molecular mechanisms that resulted in the specific reprogramming favored in constant environments. Conclusion This study provides a comprehensive understanding of a case of microbial evolution and hints at the wide dynamic range that a single fitness-enhancing mutation may display. It demonstrates how the modulation of a pleiotropic regulator can be used by cells to improve one trait while simultaneously work around other limiting constraints, by fine-tuning the expression of a wide range of cellular processes. Evolution Systems biology Lactic acid bacteria Filipe Branco dos Santos verfasserin aut Anne Hesseling verfasserin aut Jaakko J. Uusitalo verfasserin aut Herwig Bachmann verfasserin aut Vera Benavente verfasserin aut Anisha Goel verfasserin aut Jan Berkhout verfasserin aut Frank J. Bruggeman verfasserin aut Siewert-Jan Marrink verfasserin aut Manolo Montalban-Lopez verfasserin aut Anne de Jong verfasserin aut Jan Kok verfasserin aut Douwe Molenaar verfasserin aut Bert Poolman verfasserin aut Bas Teusink verfasserin aut Oscar P. Kuipers verfasserin aut In BMC Evolutionary Biology BMC, 2003 19(2019), 1, Seite 15 (DE-627)32664489X (DE-600)2041493-6 14712148 nnns volume:19 year:2019 number:1 pages:15 https://doi.org/10.1186/s12862-018-1331-x kostenfrei https://doaj.org/article/268f290335c04c76aeea5bce0f5b920b kostenfrei http://link.springer.com/article/10.1186/s12862-018-1331-x kostenfrei https://doaj.org/toc/1471-2148 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2019 1 15 |
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10.1186/s12862-018-1331-x doi (DE-627)DOAJ077162498 (DE-599)DOAJ268f290335c04c76aeea5bce0f5b920b DE-627 ger DE-627 rakwb eng QH359-425 Claire E. Price verfasserin aut Adaption to glucose limitation is modulated by the pleotropic regulator CcpA, independent of selection pressure strength 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background A central theme in (micro)biology is understanding the molecular basis of fitness i.e. which strategies are successful under which conditions; how do organisms implement such strategies at the molecular level; and which constraints shape the trade-offs between alternative strategies. Highly standardized microbial laboratory evolution experiments are ideally suited to approach these questions. For example, prolonged chemostats provide a constant environment in which the growth rate can be set, and the adaptive process of the organism to such environment can be subsequently characterized. Results We performed parallel laboratory evolution of Lactococcus lactis in chemostats varying the quantitative value of the selective pressure by imposing two different growth rates. A mutation in one specific amino acid residue of the global transcriptional regulator of carbon metabolism, CcpA, was selected in all of the evolution experiments performed. We subsequently showed that this mutation confers predictable fitness improvements at other glucose-limited growth rates as well. In silico protein structural analysis of wild type and evolved CcpA, as well as biochemical and phenotypic assays, provided the underpinning molecular mechanisms that resulted in the specific reprogramming favored in constant environments. Conclusion This study provides a comprehensive understanding of a case of microbial evolution and hints at the wide dynamic range that a single fitness-enhancing mutation may display. It demonstrates how the modulation of a pleiotropic regulator can be used by cells to improve one trait while simultaneously work around other limiting constraints, by fine-tuning the expression of a wide range of cellular processes. Evolution Systems biology Lactic acid bacteria Filipe Branco dos Santos verfasserin aut Anne Hesseling verfasserin aut Jaakko J. Uusitalo verfasserin aut Herwig Bachmann verfasserin aut Vera Benavente verfasserin aut Anisha Goel verfasserin aut Jan Berkhout verfasserin aut Frank J. Bruggeman verfasserin aut Siewert-Jan Marrink verfasserin aut Manolo Montalban-Lopez verfasserin aut Anne de Jong verfasserin aut Jan Kok verfasserin aut Douwe Molenaar verfasserin aut Bert Poolman verfasserin aut Bas Teusink verfasserin aut Oscar P. Kuipers verfasserin aut In BMC Evolutionary Biology BMC, 2003 19(2019), 1, Seite 15 (DE-627)32664489X (DE-600)2041493-6 14712148 nnns volume:19 year:2019 number:1 pages:15 https://doi.org/10.1186/s12862-018-1331-x kostenfrei https://doaj.org/article/268f290335c04c76aeea5bce0f5b920b kostenfrei http://link.springer.com/article/10.1186/s12862-018-1331-x kostenfrei https://doaj.org/toc/1471-2148 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2019 1 15 |
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Claire E. Price @@aut@@ Filipe Branco dos Santos @@aut@@ Anne Hesseling @@aut@@ Jaakko J. Uusitalo @@aut@@ Herwig Bachmann @@aut@@ Vera Benavente @@aut@@ Anisha Goel @@aut@@ Jan Berkhout @@aut@@ Frank J. Bruggeman @@aut@@ Siewert-Jan Marrink @@aut@@ Manolo Montalban-Lopez @@aut@@ Anne de Jong @@aut@@ Jan Kok @@aut@@ Douwe Molenaar @@aut@@ Bert Poolman @@aut@@ Bas Teusink @@aut@@ Oscar P. Kuipers @@aut@@ |
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QH359-425 Adaption to glucose limitation is modulated by the pleotropic regulator CcpA, independent of selection pressure strength Evolution Systems biology Lactic acid bacteria |
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Claire E. Price Filipe Branco dos Santos Anne Hesseling Jaakko J. Uusitalo Herwig Bachmann Vera Benavente Anisha Goel Jan Berkhout Frank J. Bruggeman Siewert-Jan Marrink Manolo Montalban-Lopez Anne de Jong Jan Kok Douwe Molenaar Bert Poolman Bas Teusink Oscar P. Kuipers |
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adaption to glucose limitation is modulated by the pleotropic regulator ccpa, independent of selection pressure strength |
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Adaption to glucose limitation is modulated by the pleotropic regulator CcpA, independent of selection pressure strength |
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Abstract Background A central theme in (micro)biology is understanding the molecular basis of fitness i.e. which strategies are successful under which conditions; how do organisms implement such strategies at the molecular level; and which constraints shape the trade-offs between alternative strategies. Highly standardized microbial laboratory evolution experiments are ideally suited to approach these questions. For example, prolonged chemostats provide a constant environment in which the growth rate can be set, and the adaptive process of the organism to such environment can be subsequently characterized. Results We performed parallel laboratory evolution of Lactococcus lactis in chemostats varying the quantitative value of the selective pressure by imposing two different growth rates. A mutation in one specific amino acid residue of the global transcriptional regulator of carbon metabolism, CcpA, was selected in all of the evolution experiments performed. We subsequently showed that this mutation confers predictable fitness improvements at other glucose-limited growth rates as well. In silico protein structural analysis of wild type and evolved CcpA, as well as biochemical and phenotypic assays, provided the underpinning molecular mechanisms that resulted in the specific reprogramming favored in constant environments. Conclusion This study provides a comprehensive understanding of a case of microbial evolution and hints at the wide dynamic range that a single fitness-enhancing mutation may display. It demonstrates how the modulation of a pleiotropic regulator can be used by cells to improve one trait while simultaneously work around other limiting constraints, by fine-tuning the expression of a wide range of cellular processes. |
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Abstract Background A central theme in (micro)biology is understanding the molecular basis of fitness i.e. which strategies are successful under which conditions; how do organisms implement such strategies at the molecular level; and which constraints shape the trade-offs between alternative strategies. Highly standardized microbial laboratory evolution experiments are ideally suited to approach these questions. For example, prolonged chemostats provide a constant environment in which the growth rate can be set, and the adaptive process of the organism to such environment can be subsequently characterized. Results We performed parallel laboratory evolution of Lactococcus lactis in chemostats varying the quantitative value of the selective pressure by imposing two different growth rates. A mutation in one specific amino acid residue of the global transcriptional regulator of carbon metabolism, CcpA, was selected in all of the evolution experiments performed. We subsequently showed that this mutation confers predictable fitness improvements at other glucose-limited growth rates as well. In silico protein structural analysis of wild type and evolved CcpA, as well as biochemical and phenotypic assays, provided the underpinning molecular mechanisms that resulted in the specific reprogramming favored in constant environments. Conclusion This study provides a comprehensive understanding of a case of microbial evolution and hints at the wide dynamic range that a single fitness-enhancing mutation may display. It demonstrates how the modulation of a pleiotropic regulator can be used by cells to improve one trait while simultaneously work around other limiting constraints, by fine-tuning the expression of a wide range of cellular processes. |
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Abstract Background A central theme in (micro)biology is understanding the molecular basis of fitness i.e. which strategies are successful under which conditions; how do organisms implement such strategies at the molecular level; and which constraints shape the trade-offs between alternative strategies. Highly standardized microbial laboratory evolution experiments are ideally suited to approach these questions. For example, prolonged chemostats provide a constant environment in which the growth rate can be set, and the adaptive process of the organism to such environment can be subsequently characterized. Results We performed parallel laboratory evolution of Lactococcus lactis in chemostats varying the quantitative value of the selective pressure by imposing two different growth rates. A mutation in one specific amino acid residue of the global transcriptional regulator of carbon metabolism, CcpA, was selected in all of the evolution experiments performed. We subsequently showed that this mutation confers predictable fitness improvements at other glucose-limited growth rates as well. In silico protein structural analysis of wild type and evolved CcpA, as well as biochemical and phenotypic assays, provided the underpinning molecular mechanisms that resulted in the specific reprogramming favored in constant environments. Conclusion This study provides a comprehensive understanding of a case of microbial evolution and hints at the wide dynamic range that a single fitness-enhancing mutation may display. It demonstrates how the modulation of a pleiotropic regulator can be used by cells to improve one trait while simultaneously work around other limiting constraints, by fine-tuning the expression of a wide range of cellular processes. |
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Adaption to glucose limitation is modulated by the pleotropic regulator CcpA, independent of selection pressure strength |
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