Cellular transcripts regulated during infections with Highly Pathogenic H5N1 Avian Influenza virus in 3 host systems

<p<Abstract</p< <p<Background</p< <p<Highly pathogenic Avian Influenza (HPAI) virus is able to infect many hosts and the virus replicates in high levels in the respiratory tract inducing severe lung lesions. The pathogenesis of the disease is actually the outcome of the infection as determined by complex host-virus interactions involving the functional kinetics of large numbers of participating genes. Understanding the genes and proteins involved in host cellular responses are therefore, critical for the elucidation of the mechanisms of infection.</p< <p<Methods</p< <p<Differentially expressed transcripts regulated in a H5N1 infections of whole lung organ of chicken, <it<in-vitro </it<chick embryo lung primary cell culture (CeLu) and a continuous Madin Darby Canine Kidney cell line was undertaken. An improved mRNA differential display technique (Gene Fishing™) using annealing control primers that generates reproducible, authentic and long PCR products that are detectable on agarose gels was used for the identification of differentially expressed genes (DEGs). Seven of the genes have been selected for validation using a TaqMan<sup<® </sup<based real time quantitative PCR assay.</p< <p<Results</p< <p<Thirty seven known and unique differentially expressed genes from lungs of chickens, CeLu and MDCK cells were isolated. Among the genes isolated and identified include heat shock proteins, Cyclin D2, Prenyl (decaprenyl) diphosphate synthase, IL-8 and many other unknown genes. The quantitative real time RT-PCR assay data showed that the transcription kinetics of the selected genes were clearly altered during infection by the Highly Pathogenic Avian Influenza virus.</p< <p<Conclusion</p< <p<The Gene Fishing™ technique has allowed for the first time, the isolation and identification of sequences of host cellular genes regulated during H5N1 virus infection. In this limited study, the differentially expressed genes in the three host systems were not identical, thus suggesting that their responses to the H5N1 infection may not share similar mechanisms and pathways.</p< Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Noor Suriani M [verfasserIn] Mohamed Maizan [verfasserIn] Omar Abdul R [verfasserIn] Hassan Sharifah S [verfasserIn] Balasubramaniam Vinod RMT [verfasserIn] Mohamed Ramlan [verfasserIn] Othman Iekhsan [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2011

Schlagwörter:	Avian Influenza virus mRNA differential display annealing control primer hsp60 gene cyclin D2 gene Interleukin 8 gene

Übergeordnetes Werk:	In: Virology Journal - BMC, 2004, 8(2011), 1, p 196
Übergeordnetes Werk:	volume:8 ; year:2011 ; number:1, p 196

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/1743-422X-8-196

Katalog-ID:	DOAJ077429761

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520			\|a <p<Abstract</p< <p<Background</p< <p<Highly pathogenic Avian Influenza (HPAI) virus is able to infect many hosts and the virus replicates in high levels in the respiratory tract inducing severe lung lesions. The pathogenesis of the disease is actually the outcome of the infection as determined by complex host-virus interactions involving the functional kinetics of large numbers of participating genes. Understanding the genes and proteins involved in host cellular responses are therefore, critical for the elucidation of the mechanisms of infection.</p< <p<Methods</p< <p<Differentially expressed transcripts regulated in a H5N1 infections of whole lung organ of chicken, <it<in-vitro </it<chick embryo lung primary cell culture (CeLu) and a continuous Madin Darby Canine Kidney cell line was undertaken. An improved mRNA differential display technique (Gene Fishing™) using annealing control primers that generates reproducible, authentic and long PCR products that are detectable on agarose gels was used for the identification of differentially expressed genes (DEGs). Seven of the genes have been selected for validation using a TaqMan<sup<® </sup<based real time quantitative PCR assay.</p< <p<Results</p< <p<Thirty seven known and unique differentially expressed genes from lungs of chickens, CeLu and MDCK cells were isolated. Among the genes isolated and identified include heat shock proteins, Cyclin D2, Prenyl (decaprenyl) diphosphate synthase, IL-8 and many other unknown genes. The quantitative real time RT-PCR assay data showed that the transcription kinetics of the selected genes were clearly altered during infection by the Highly Pathogenic Avian Influenza virus.</p< <p<Conclusion</p< <p<The Gene Fishing™ technique has allowed for the first time, the isolation and identification of sequences of host cellular genes regulated during H5N1 virus infection. In this limited study, the differentially expressed genes in the three host systems were not identical, thus suggesting that their responses to the H5N1 infection may not share similar mechanisms and pathways.</p<
650		4	\|a Avian Influenza virus
650		4	\|a mRNA differential display
650		4	\|a annealing control primer
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650		4	\|a cyclin D2 gene
650		4	\|a Interleukin 8 gene
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700	0		\|a Omar Abdul R \|e verfasserin \|4 aut
700	0		\|a Hassan Sharifah S \|e verfasserin \|4 aut
700	0		\|a Balasubramaniam Vinod RMT \|e verfasserin \|4 aut
700	0		\|a Mohamed Ramlan \|e verfasserin \|4 aut
700	0		\|a Othman Iekhsan \|e verfasserin \|4 aut
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matchkey_str	article:1743422X:2011----::ellrrncitrgltduignetosihihyahgnc51vai
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publishDate	2011
allfields	10.1186/1743-422X-8-196 doi (DE-627)DOAJ077429761 (DE-599)DOAJ80486ebaa77342ba87db18c0f7e2e4ea DE-627 ger DE-627 rakwb eng RC109-216 Noor Suriani M verfasserin aut Cellular transcripts regulated during infections with Highly Pathogenic H5N1 Avian Influenza virus in 3 host systems 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Highly pathogenic Avian Influenza (HPAI) virus is able to infect many hosts and the virus replicates in high levels in the respiratory tract inducing severe lung lesions. The pathogenesis of the disease is actually the outcome of the infection as determined by complex host-virus interactions involving the functional kinetics of large numbers of participating genes. Understanding the genes and proteins involved in host cellular responses are therefore, critical for the elucidation of the mechanisms of infection.</p< <p<Methods</p< <p<Differentially expressed transcripts regulated in a H5N1 infections of whole lung organ of chicken, <it<in-vitro </it<chick embryo lung primary cell culture (CeLu) and a continuous Madin Darby Canine Kidney cell line was undertaken. An improved mRNA differential display technique (Gene Fishing™) using annealing control primers that generates reproducible, authentic and long PCR products that are detectable on agarose gels was used for the identification of differentially expressed genes (DEGs). Seven of the genes have been selected for validation using a TaqMan<sup<® </sup<based real time quantitative PCR assay.</p< <p<Results</p< <p<Thirty seven known and unique differentially expressed genes from lungs of chickens, CeLu and MDCK cells were isolated. Among the genes isolated and identified include heat shock proteins, Cyclin D2, Prenyl (decaprenyl) diphosphate synthase, IL-8 and many other unknown genes. The quantitative real time RT-PCR assay data showed that the transcription kinetics of the selected genes were clearly altered during infection by the Highly Pathogenic Avian Influenza virus.</p< <p<Conclusion</p< <p<The Gene Fishing™ technique has allowed for the first time, the isolation and identification of sequences of host cellular genes regulated during H5N1 virus infection. In this limited study, the differentially expressed genes in the three host systems were not identical, thus suggesting that their responses to the H5N1 infection may not share similar mechanisms and pathways.</p< Avian Influenza virus mRNA differential display annealing control primer hsp60 gene cyclin D2 gene Interleukin 8 gene Infectious and parasitic diseases Mohamed Maizan verfasserin aut Omar Abdul R verfasserin aut Hassan Sharifah S verfasserin aut Balasubramaniam Vinod RMT verfasserin aut Mohamed Ramlan verfasserin aut Othman Iekhsan verfasserin aut In Virology Journal BMC, 2004 8(2011), 1, p 196 (DE-627)394165004 (DE-600)2160640-7 1743422X nnns volume:8 year:2011 number:1, p 196 https://doi.org/10.1186/1743-422X-8-196 kostenfrei https://doaj.org/article/80486ebaa77342ba87db18c0f7e2e4ea kostenfrei http://www.virologyj.com/content/8/1/196 kostenfrei https://doaj.org/toc/1743-422X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2011 1, p 196
spelling	10.1186/1743-422X-8-196 doi (DE-627)DOAJ077429761 (DE-599)DOAJ80486ebaa77342ba87db18c0f7e2e4ea DE-627 ger DE-627 rakwb eng RC109-216 Noor Suriani M verfasserin aut Cellular transcripts regulated during infections with Highly Pathogenic H5N1 Avian Influenza virus in 3 host systems 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Highly pathogenic Avian Influenza (HPAI) virus is able to infect many hosts and the virus replicates in high levels in the respiratory tract inducing severe lung lesions. The pathogenesis of the disease is actually the outcome of the infection as determined by complex host-virus interactions involving the functional kinetics of large numbers of participating genes. Understanding the genes and proteins involved in host cellular responses are therefore, critical for the elucidation of the mechanisms of infection.</p< <p<Methods</p< <p<Differentially expressed transcripts regulated in a H5N1 infections of whole lung organ of chicken, <it<in-vitro </it<chick embryo lung primary cell culture (CeLu) and a continuous Madin Darby Canine Kidney cell line was undertaken. An improved mRNA differential display technique (Gene Fishing™) using annealing control primers that generates reproducible, authentic and long PCR products that are detectable on agarose gels was used for the identification of differentially expressed genes (DEGs). Seven of the genes have been selected for validation using a TaqMan<sup<® </sup<based real time quantitative PCR assay.</p< <p<Results</p< <p<Thirty seven known and unique differentially expressed genes from lungs of chickens, CeLu and MDCK cells were isolated. Among the genes isolated and identified include heat shock proteins, Cyclin D2, Prenyl (decaprenyl) diphosphate synthase, IL-8 and many other unknown genes. The quantitative real time RT-PCR assay data showed that the transcription kinetics of the selected genes were clearly altered during infection by the Highly Pathogenic Avian Influenza virus.</p< <p<Conclusion</p< <p<The Gene Fishing™ technique has allowed for the first time, the isolation and identification of sequences of host cellular genes regulated during H5N1 virus infection. In this limited study, the differentially expressed genes in the three host systems were not identical, thus suggesting that their responses to the H5N1 infection may not share similar mechanisms and pathways.</p< Avian Influenza virus mRNA differential display annealing control primer hsp60 gene cyclin D2 gene Interleukin 8 gene Infectious and parasitic diseases Mohamed Maizan verfasserin aut Omar Abdul R verfasserin aut Hassan Sharifah S verfasserin aut Balasubramaniam Vinod RMT verfasserin aut Mohamed Ramlan verfasserin aut Othman Iekhsan verfasserin aut In Virology Journal BMC, 2004 8(2011), 1, p 196 (DE-627)394165004 (DE-600)2160640-7 1743422X nnns volume:8 year:2011 number:1, p 196 https://doi.org/10.1186/1743-422X-8-196 kostenfrei https://doaj.org/article/80486ebaa77342ba87db18c0f7e2e4ea kostenfrei http://www.virologyj.com/content/8/1/196 kostenfrei https://doaj.org/toc/1743-422X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2011 1, p 196
allfields_unstemmed	10.1186/1743-422X-8-196 doi (DE-627)DOAJ077429761 (DE-599)DOAJ80486ebaa77342ba87db18c0f7e2e4ea DE-627 ger DE-627 rakwb eng RC109-216 Noor Suriani M verfasserin aut Cellular transcripts regulated during infections with Highly Pathogenic H5N1 Avian Influenza virus in 3 host systems 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Highly pathogenic Avian Influenza (HPAI) virus is able to infect many hosts and the virus replicates in high levels in the respiratory tract inducing severe lung lesions. The pathogenesis of the disease is actually the outcome of the infection as determined by complex host-virus interactions involving the functional kinetics of large numbers of participating genes. Understanding the genes and proteins involved in host cellular responses are therefore, critical for the elucidation of the mechanisms of infection.</p< <p<Methods</p< <p<Differentially expressed transcripts regulated in a H5N1 infections of whole lung organ of chicken, <it<in-vitro </it<chick embryo lung primary cell culture (CeLu) and a continuous Madin Darby Canine Kidney cell line was undertaken. An improved mRNA differential display technique (Gene Fishing™) using annealing control primers that generates reproducible, authentic and long PCR products that are detectable on agarose gels was used for the identification of differentially expressed genes (DEGs). Seven of the genes have been selected for validation using a TaqMan<sup<® </sup<based real time quantitative PCR assay.</p< <p<Results</p< <p<Thirty seven known and unique differentially expressed genes from lungs of chickens, CeLu and MDCK cells were isolated. Among the genes isolated and identified include heat shock proteins, Cyclin D2, Prenyl (decaprenyl) diphosphate synthase, IL-8 and many other unknown genes. The quantitative real time RT-PCR assay data showed that the transcription kinetics of the selected genes were clearly altered during infection by the Highly Pathogenic Avian Influenza virus.</p< <p<Conclusion</p< <p<The Gene Fishing™ technique has allowed for the first time, the isolation and identification of sequences of host cellular genes regulated during H5N1 virus infection. In this limited study, the differentially expressed genes in the three host systems were not identical, thus suggesting that their responses to the H5N1 infection may not share similar mechanisms and pathways.</p< Avian Influenza virus mRNA differential display annealing control primer hsp60 gene cyclin D2 gene Interleukin 8 gene Infectious and parasitic diseases Mohamed Maizan verfasserin aut Omar Abdul R verfasserin aut Hassan Sharifah S verfasserin aut Balasubramaniam Vinod RMT verfasserin aut Mohamed Ramlan verfasserin aut Othman Iekhsan verfasserin aut In Virology Journal BMC, 2004 8(2011), 1, p 196 (DE-627)394165004 (DE-600)2160640-7 1743422X nnns volume:8 year:2011 number:1, p 196 https://doi.org/10.1186/1743-422X-8-196 kostenfrei https://doaj.org/article/80486ebaa77342ba87db18c0f7e2e4ea kostenfrei http://www.virologyj.com/content/8/1/196 kostenfrei https://doaj.org/toc/1743-422X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2011 1, p 196
allfieldsGer	10.1186/1743-422X-8-196 doi (DE-627)DOAJ077429761 (DE-599)DOAJ80486ebaa77342ba87db18c0f7e2e4ea DE-627 ger DE-627 rakwb eng RC109-216 Noor Suriani M verfasserin aut Cellular transcripts regulated during infections with Highly Pathogenic H5N1 Avian Influenza virus in 3 host systems 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Highly pathogenic Avian Influenza (HPAI) virus is able to infect many hosts and the virus replicates in high levels in the respiratory tract inducing severe lung lesions. The pathogenesis of the disease is actually the outcome of the infection as determined by complex host-virus interactions involving the functional kinetics of large numbers of participating genes. Understanding the genes and proteins involved in host cellular responses are therefore, critical for the elucidation of the mechanisms of infection.</p< <p<Methods</p< <p<Differentially expressed transcripts regulated in a H5N1 infections of whole lung organ of chicken, <it<in-vitro </it<chick embryo lung primary cell culture (CeLu) and a continuous Madin Darby Canine Kidney cell line was undertaken. An improved mRNA differential display technique (Gene Fishing™) using annealing control primers that generates reproducible, authentic and long PCR products that are detectable on agarose gels was used for the identification of differentially expressed genes (DEGs). Seven of the genes have been selected for validation using a TaqMan<sup<® </sup<based real time quantitative PCR assay.</p< <p<Results</p< <p<Thirty seven known and unique differentially expressed genes from lungs of chickens, CeLu and MDCK cells were isolated. Among the genes isolated and identified include heat shock proteins, Cyclin D2, Prenyl (decaprenyl) diphosphate synthase, IL-8 and many other unknown genes. The quantitative real time RT-PCR assay data showed that the transcription kinetics of the selected genes were clearly altered during infection by the Highly Pathogenic Avian Influenza virus.</p< <p<Conclusion</p< <p<The Gene Fishing™ technique has allowed for the first time, the isolation and identification of sequences of host cellular genes regulated during H5N1 virus infection. In this limited study, the differentially expressed genes in the three host systems were not identical, thus suggesting that their responses to the H5N1 infection may not share similar mechanisms and pathways.</p< Avian Influenza virus mRNA differential display annealing control primer hsp60 gene cyclin D2 gene Interleukin 8 gene Infectious and parasitic diseases Mohamed Maizan verfasserin aut Omar Abdul R verfasserin aut Hassan Sharifah S verfasserin aut Balasubramaniam Vinod RMT verfasserin aut Mohamed Ramlan verfasserin aut Othman Iekhsan verfasserin aut In Virology Journal BMC, 2004 8(2011), 1, p 196 (DE-627)394165004 (DE-600)2160640-7 1743422X nnns volume:8 year:2011 number:1, p 196 https://doi.org/10.1186/1743-422X-8-196 kostenfrei https://doaj.org/article/80486ebaa77342ba87db18c0f7e2e4ea kostenfrei http://www.virologyj.com/content/8/1/196 kostenfrei https://doaj.org/toc/1743-422X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2011 1, p 196
allfieldsSound	10.1186/1743-422X-8-196 doi (DE-627)DOAJ077429761 (DE-599)DOAJ80486ebaa77342ba87db18c0f7e2e4ea DE-627 ger DE-627 rakwb eng RC109-216 Noor Suriani M verfasserin aut Cellular transcripts regulated during infections with Highly Pathogenic H5N1 Avian Influenza virus in 3 host systems 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Highly pathogenic Avian Influenza (HPAI) virus is able to infect many hosts and the virus replicates in high levels in the respiratory tract inducing severe lung lesions. The pathogenesis of the disease is actually the outcome of the infection as determined by complex host-virus interactions involving the functional kinetics of large numbers of participating genes. Understanding the genes and proteins involved in host cellular responses are therefore, critical for the elucidation of the mechanisms of infection.</p< <p<Methods</p< <p<Differentially expressed transcripts regulated in a H5N1 infections of whole lung organ of chicken, <it<in-vitro </it<chick embryo lung primary cell culture (CeLu) and a continuous Madin Darby Canine Kidney cell line was undertaken. An improved mRNA differential display technique (Gene Fishing™) using annealing control primers that generates reproducible, authentic and long PCR products that are detectable on agarose gels was used for the identification of differentially expressed genes (DEGs). Seven of the genes have been selected for validation using a TaqMan<sup<® </sup<based real time quantitative PCR assay.</p< <p<Results</p< <p<Thirty seven known and unique differentially expressed genes from lungs of chickens, CeLu and MDCK cells were isolated. Among the genes isolated and identified include heat shock proteins, Cyclin D2, Prenyl (decaprenyl) diphosphate synthase, IL-8 and many other unknown genes. The quantitative real time RT-PCR assay data showed that the transcription kinetics of the selected genes were clearly altered during infection by the Highly Pathogenic Avian Influenza virus.</p< <p<Conclusion</p< <p<The Gene Fishing™ technique has allowed for the first time, the isolation and identification of sequences of host cellular genes regulated during H5N1 virus infection. In this limited study, the differentially expressed genes in the three host systems were not identical, thus suggesting that their responses to the H5N1 infection may not share similar mechanisms and pathways.</p< Avian Influenza virus mRNA differential display annealing control primer hsp60 gene cyclin D2 gene Interleukin 8 gene Infectious and parasitic diseases Mohamed Maizan verfasserin aut Omar Abdul R verfasserin aut Hassan Sharifah S verfasserin aut Balasubramaniam Vinod RMT verfasserin aut Mohamed Ramlan verfasserin aut Othman Iekhsan verfasserin aut In Virology Journal BMC, 2004 8(2011), 1, p 196 (DE-627)394165004 (DE-600)2160640-7 1743422X nnns volume:8 year:2011 number:1, p 196 https://doi.org/10.1186/1743-422X-8-196 kostenfrei https://doaj.org/article/80486ebaa77342ba87db18c0f7e2e4ea kostenfrei http://www.virologyj.com/content/8/1/196 kostenfrei https://doaj.org/toc/1743-422X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2011 1, p 196
language	English
source	In Virology Journal 8(2011), 1, p 196 volume:8 year:2011 number:1, p 196
sourceStr	In Virology Journal 8(2011), 1, p 196 volume:8 year:2011 number:1, p 196
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Avian Influenza virus mRNA differential display annealing control primer hsp60 gene cyclin D2 gene Interleukin 8 gene Infectious and parasitic diseases
isfreeaccess_bool	true
container_title	Virology Journal
authorswithroles_txt_mv	Noor Suriani M @@aut@@ Mohamed Maizan @@aut@@ Omar Abdul R @@aut@@ Hassan Sharifah S @@aut@@ Balasubramaniam Vinod RMT @@aut@@ Mohamed Ramlan @@aut@@ Othman Iekhsan @@aut@@
publishDateDaySort_date	2011-01-01T00:00:00Z
hierarchy_top_id	394165004
id	DOAJ077429761
language_de	englisch
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The pathogenesis of the disease is actually the outcome of the infection as determined by complex host-virus interactions involving the functional kinetics of large numbers of participating genes. Understanding the genes and proteins involved in host cellular responses are therefore, critical for the elucidation of the mechanisms of infection.</p< <p<Methods</p< <p<Differentially expressed transcripts regulated in a H5N1 infections of whole lung organ of chicken, <it<in-vitro </it<chick embryo lung primary cell culture (CeLu) and a continuous Madin Darby Canine Kidney cell line was undertaken. An improved mRNA differential display technique (Gene Fishing™) using annealing control primers that generates reproducible, authentic and long PCR products that are detectable on agarose gels was used for the identification of differentially expressed genes (DEGs). 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callnumber-first	R - Medicine
author	Noor Suriani M
spellingShingle	Noor Suriani M misc RC109-216 misc Avian Influenza virus misc mRNA differential display misc annealing control primer misc hsp60 gene misc cyclin D2 gene misc Interleukin 8 gene misc Infectious and parasitic diseases Cellular transcripts regulated during infections with Highly Pathogenic H5N1 Avian Influenza virus in 3 host systems
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topic_title	RC109-216 Cellular transcripts regulated during infections with Highly Pathogenic H5N1 Avian Influenza virus in 3 host systems Avian Influenza virus mRNA differential display annealing control primer hsp60 gene cyclin D2 gene Interleukin 8 gene
topic	misc RC109-216 misc Avian Influenza virus misc mRNA differential display misc annealing control primer misc hsp60 gene misc cyclin D2 gene misc Interleukin 8 gene misc Infectious and parasitic diseases
topic_unstemmed	misc RC109-216 misc Avian Influenza virus misc mRNA differential display misc annealing control primer misc hsp60 gene misc cyclin D2 gene misc Interleukin 8 gene misc Infectious and parasitic diseases
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title	Cellular transcripts regulated during infections with Highly Pathogenic H5N1 Avian Influenza virus in 3 host systems
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title_full	Cellular transcripts regulated during infections with Highly Pathogenic H5N1 Avian Influenza virus in 3 host systems
author_sort	Noor Suriani M
journal	Virology Journal
journalStr	Virology Journal
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author_browse	Noor Suriani M Mohamed Maizan Omar Abdul R Hassan Sharifah S Balasubramaniam Vinod RMT Mohamed Ramlan Othman Iekhsan
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title_sort	cellular transcripts regulated during infections with highly pathogenic h5n1 avian influenza virus in 3 host systems
callnumber	RC109-216
title_auth	Cellular transcripts regulated during infections with Highly Pathogenic H5N1 Avian Influenza virus in 3 host systems
abstract	<p<Abstract</p< <p<Background</p< <p<Highly pathogenic Avian Influenza (HPAI) virus is able to infect many hosts and the virus replicates in high levels in the respiratory tract inducing severe lung lesions. The pathogenesis of the disease is actually the outcome of the infection as determined by complex host-virus interactions involving the functional kinetics of large numbers of participating genes. Understanding the genes and proteins involved in host cellular responses are therefore, critical for the elucidation of the mechanisms of infection.</p< <p<Methods</p< <p<Differentially expressed transcripts regulated in a H5N1 infections of whole lung organ of chicken, <it<in-vitro </it<chick embryo lung primary cell culture (CeLu) and a continuous Madin Darby Canine Kidney cell line was undertaken. An improved mRNA differential display technique (Gene Fishing™) using annealing control primers that generates reproducible, authentic and long PCR products that are detectable on agarose gels was used for the identification of differentially expressed genes (DEGs). Seven of the genes have been selected for validation using a TaqMan<sup<® </sup<based real time quantitative PCR assay.</p< <p<Results</p< <p<Thirty seven known and unique differentially expressed genes from lungs of chickens, CeLu and MDCK cells were isolated. Among the genes isolated and identified include heat shock proteins, Cyclin D2, Prenyl (decaprenyl) diphosphate synthase, IL-8 and many other unknown genes. The quantitative real time RT-PCR assay data showed that the transcription kinetics of the selected genes were clearly altered during infection by the Highly Pathogenic Avian Influenza virus.</p< <p<Conclusion</p< <p<The Gene Fishing™ technique has allowed for the first time, the isolation and identification of sequences of host cellular genes regulated during H5N1 virus infection. In this limited study, the differentially expressed genes in the three host systems were not identical, thus suggesting that their responses to the H5N1 infection may not share similar mechanisms and pathways.</p<
abstractGer	<p<Abstract</p< <p<Background</p< <p<Highly pathogenic Avian Influenza (HPAI) virus is able to infect many hosts and the virus replicates in high levels in the respiratory tract inducing severe lung lesions. The pathogenesis of the disease is actually the outcome of the infection as determined by complex host-virus interactions involving the functional kinetics of large numbers of participating genes. Understanding the genes and proteins involved in host cellular responses are therefore, critical for the elucidation of the mechanisms of infection.</p< <p<Methods</p< <p<Differentially expressed transcripts regulated in a H5N1 infections of whole lung organ of chicken, <it<in-vitro </it<chick embryo lung primary cell culture (CeLu) and a continuous Madin Darby Canine Kidney cell line was undertaken. An improved mRNA differential display technique (Gene Fishing™) using annealing control primers that generates reproducible, authentic and long PCR products that are detectable on agarose gels was used for the identification of differentially expressed genes (DEGs). Seven of the genes have been selected for validation using a TaqMan<sup<® </sup<based real time quantitative PCR assay.</p< <p<Results</p< <p<Thirty seven known and unique differentially expressed genes from lungs of chickens, CeLu and MDCK cells were isolated. Among the genes isolated and identified include heat shock proteins, Cyclin D2, Prenyl (decaprenyl) diphosphate synthase, IL-8 and many other unknown genes. The quantitative real time RT-PCR assay data showed that the transcription kinetics of the selected genes were clearly altered during infection by the Highly Pathogenic Avian Influenza virus.</p< <p<Conclusion</p< <p<The Gene Fishing™ technique has allowed for the first time, the isolation and identification of sequences of host cellular genes regulated during H5N1 virus infection. In this limited study, the differentially expressed genes in the three host systems were not identical, thus suggesting that their responses to the H5N1 infection may not share similar mechanisms and pathways.</p<
abstract_unstemmed	<p<Abstract</p< <p<Background</p< <p<Highly pathogenic Avian Influenza (HPAI) virus is able to infect many hosts and the virus replicates in high levels in the respiratory tract inducing severe lung lesions. The pathogenesis of the disease is actually the outcome of the infection as determined by complex host-virus interactions involving the functional kinetics of large numbers of participating genes. Understanding the genes and proteins involved in host cellular responses are therefore, critical for the elucidation of the mechanisms of infection.</p< <p<Methods</p< <p<Differentially expressed transcripts regulated in a H5N1 infections of whole lung organ of chicken, <it<in-vitro </it<chick embryo lung primary cell culture (CeLu) and a continuous Madin Darby Canine Kidney cell line was undertaken. An improved mRNA differential display technique (Gene Fishing™) using annealing control primers that generates reproducible, authentic and long PCR products that are detectable on agarose gels was used for the identification of differentially expressed genes (DEGs). Seven of the genes have been selected for validation using a TaqMan<sup<® </sup<based real time quantitative PCR assay.</p< <p<Results</p< <p<Thirty seven known and unique differentially expressed genes from lungs of chickens, CeLu and MDCK cells were isolated. Among the genes isolated and identified include heat shock proteins, Cyclin D2, Prenyl (decaprenyl) diphosphate synthase, IL-8 and many other unknown genes. The quantitative real time RT-PCR assay data showed that the transcription kinetics of the selected genes were clearly altered during infection by the Highly Pathogenic Avian Influenza virus.</p< <p<Conclusion</p< <p<The Gene Fishing™ technique has allowed for the first time, the isolation and identification of sequences of host cellular genes regulated during H5N1 virus infection. In this limited study, the differentially expressed genes in the three host systems were not identical, thus suggesting that their responses to the H5N1 infection may not share similar mechanisms and pathways.</p<
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title_short	Cellular transcripts regulated during infections with Highly Pathogenic H5N1 Avian Influenza virus in 3 host systems
url	https://doi.org/10.1186/1743-422X-8-196 https://doaj.org/article/80486ebaa77342ba87db18c0f7e2e4ea http://www.virologyj.com/content/8/1/196 https://doaj.org/toc/1743-422X
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Seven of the genes have been selected for validation using a TaqMan<sup<® </sup<based real time quantitative PCR assay.</p< <p<Results</p< <p<Thirty seven known and unique differentially expressed genes from lungs of chickens, CeLu and MDCK cells were isolated. Among the genes isolated and identified include heat shock proteins, Cyclin D2, Prenyl (decaprenyl) diphosphate synthase, IL-8 and many other unknown genes. The quantitative real time RT-PCR assay data showed that the transcription kinetics of the selected genes were clearly altered during infection by the Highly Pathogenic Avian Influenza virus.</p< <p<Conclusion</p< <p<The Gene Fishing™ technique has allowed for the first time, the isolation and identification of sequences of host cellular genes regulated during H5N1 virus infection. 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Cellular transcripts regulated during infections with Highly Pathogenic H5N1 Avian Influenza virus in 3 host systems

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