Molecular evidence of Borrelia burgdorferi sensu lato in patients in Brazilian central-western region
Abstract We aimed to detect DNA of Borrelia burgdorferi in whole blood and serum samples of patients with clinical symptoms and epidemiology compatible with Brazilian Lyme-like disease. Four patients with positive epidemiological histories were recruited for the study. Blood samples were collected,...
Ausführliche Beschreibung
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| Autor*in: |
Fernando Aguilar Lopes [verfasserIn] Jania de Rezende [verfasserIn] Danielly Beraldo dos Santos Silva [verfasserIn] Fernanda de Cássia Gonçalves Alves [verfasserIn] Carina Elisei de Oliveira [verfasserIn] Izaías Pereira da Costa [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch ; Spanisch ; Portugiesisch |
| Schlagwörter: |
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| Übergeordnetes Werk: |
In: Revista Brasileira de Reumatologia - Sociedade Brasileira de Reumatologia, 2005 |
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| Links: |
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| DOI / URN: |
10.1016/j.rbre.2017.05.001 |
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DOAJ077730305 |
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10.1016/j.rbre.2017.05.001 doi (DE-627)DOAJ077730305 (DE-599)DOAJ4672b52780d947b0a76c48973fd5fbb7 DE-627 ger DE-627 rakwb eng spa por RC925-935 Fernando Aguilar Lopes verfasserin aut Molecular evidence of Borrelia burgdorferi sensu lato in patients in Brazilian central-western region Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We aimed to detect DNA of Borrelia burgdorferi in whole blood and serum samples of patients with clinical symptoms and epidemiology compatible with Brazilian Lyme-like disease. Four patients with positive epidemiological histories were recruited for the study. Blood samples were collected, screened by serologic testing by ELISA and Western blotting and molecular identification of B. burgdorferi by amplifying a fragment of the conserved gene that synthesizes the hook flagellar flgE. The results showed positive serology and for the first time, the presence of B. burgdorferi sensu lato in humans in the Midwest region of Brazil. The resulting sequences were similar to GenBank corresponding sequences of B. burgdorferi flgE gene. By neighbor-joining the phylogenetic analysis, the flgE sequence of the Brazilian strain clustered in a monophyletic group with the sequence of B. burgdorferi sensu lato under 100% bootstrap support. This study opens up promising perspectives and reinforces the need for additional studies to determine the epidemiological characteristics of the disease, as well as the impact of the prevalence of Brazilian borreliosis in Mato Grosso do Sul State, Brazil. Borrelia burgdorferi Lyme disease flgE Baggio-Yoshinari syndrome Brazil Diseases of the musculoskeletal system Jania de Rezende verfasserin aut Danielly Beraldo dos Santos Silva verfasserin aut Fernanda de Cássia Gonçalves Alves verfasserin aut Carina Elisei de Oliveira verfasserin aut Izaías Pereira da Costa verfasserin aut In Revista Brasileira de Reumatologia Sociedade Brasileira de Reumatologia, 2005 (DE-627)508330734 (DE-600)2223192-4 18094570 nnns https://doi.org/10.1016/j.rbre.2017.05.001 kostenfrei https://doaj.org/article/4672b52780d947b0a76c48973fd5fbb7 kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0482-50042017000600641&lng=en&tlng=en kostenfrei https://doaj.org/toc/1809-4570 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR |
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10.1016/j.rbre.2017.05.001 doi (DE-627)DOAJ077730305 (DE-599)DOAJ4672b52780d947b0a76c48973fd5fbb7 DE-627 ger DE-627 rakwb eng spa por RC925-935 Fernando Aguilar Lopes verfasserin aut Molecular evidence of Borrelia burgdorferi sensu lato in patients in Brazilian central-western region Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We aimed to detect DNA of Borrelia burgdorferi in whole blood and serum samples of patients with clinical symptoms and epidemiology compatible with Brazilian Lyme-like disease. Four patients with positive epidemiological histories were recruited for the study. Blood samples were collected, screened by serologic testing by ELISA and Western blotting and molecular identification of B. burgdorferi by amplifying a fragment of the conserved gene that synthesizes the hook flagellar flgE. The results showed positive serology and for the first time, the presence of B. burgdorferi sensu lato in humans in the Midwest region of Brazil. The resulting sequences were similar to GenBank corresponding sequences of B. burgdorferi flgE gene. By neighbor-joining the phylogenetic analysis, the flgE sequence of the Brazilian strain clustered in a monophyletic group with the sequence of B. burgdorferi sensu lato under 100% bootstrap support. This study opens up promising perspectives and reinforces the need for additional studies to determine the epidemiological characteristics of the disease, as well as the impact of the prevalence of Brazilian borreliosis in Mato Grosso do Sul State, Brazil. Borrelia burgdorferi Lyme disease flgE Baggio-Yoshinari syndrome Brazil Diseases of the musculoskeletal system Jania de Rezende verfasserin aut Danielly Beraldo dos Santos Silva verfasserin aut Fernanda de Cássia Gonçalves Alves verfasserin aut Carina Elisei de Oliveira verfasserin aut Izaías Pereira da Costa verfasserin aut In Revista Brasileira de Reumatologia Sociedade Brasileira de Reumatologia, 2005 (DE-627)508330734 (DE-600)2223192-4 18094570 nnns https://doi.org/10.1016/j.rbre.2017.05.001 kostenfrei https://doaj.org/article/4672b52780d947b0a76c48973fd5fbb7 kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0482-50042017000600641&lng=en&tlng=en kostenfrei https://doaj.org/toc/1809-4570 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR |
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Molecular evidence of Borrelia burgdorferi sensu lato in patients in Brazilian central-western region |
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Fernando Aguilar Lopes |
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Revista Brasileira de Reumatologia |
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Fernando Aguilar Lopes Jania de Rezende Danielly Beraldo dos Santos Silva Fernanda de Cássia Gonçalves Alves Carina Elisei de Oliveira Izaías Pereira da Costa |
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Elektronische Aufsätze |
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Fernando Aguilar Lopes |
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10.1016/j.rbre.2017.05.001 |
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verfasserin |
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molecular evidence of borrelia burgdorferi sensu lato in patients in brazilian central-western region |
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RC925-935 |
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Molecular evidence of Borrelia burgdorferi sensu lato in patients in Brazilian central-western region |
| abstract |
Abstract We aimed to detect DNA of Borrelia burgdorferi in whole blood and serum samples of patients with clinical symptoms and epidemiology compatible with Brazilian Lyme-like disease. Four patients with positive epidemiological histories were recruited for the study. Blood samples were collected, screened by serologic testing by ELISA and Western blotting and molecular identification of B. burgdorferi by amplifying a fragment of the conserved gene that synthesizes the hook flagellar flgE. The results showed positive serology and for the first time, the presence of B. burgdorferi sensu lato in humans in the Midwest region of Brazil. The resulting sequences were similar to GenBank corresponding sequences of B. burgdorferi flgE gene. By neighbor-joining the phylogenetic analysis, the flgE sequence of the Brazilian strain clustered in a monophyletic group with the sequence of B. burgdorferi sensu lato under 100% bootstrap support. This study opens up promising perspectives and reinforces the need for additional studies to determine the epidemiological characteristics of the disease, as well as the impact of the prevalence of Brazilian borreliosis in Mato Grosso do Sul State, Brazil. |
| abstractGer |
Abstract We aimed to detect DNA of Borrelia burgdorferi in whole blood and serum samples of patients with clinical symptoms and epidemiology compatible with Brazilian Lyme-like disease. Four patients with positive epidemiological histories were recruited for the study. Blood samples were collected, screened by serologic testing by ELISA and Western blotting and molecular identification of B. burgdorferi by amplifying a fragment of the conserved gene that synthesizes the hook flagellar flgE. The results showed positive serology and for the first time, the presence of B. burgdorferi sensu lato in humans in the Midwest region of Brazil. The resulting sequences were similar to GenBank corresponding sequences of B. burgdorferi flgE gene. By neighbor-joining the phylogenetic analysis, the flgE sequence of the Brazilian strain clustered in a monophyletic group with the sequence of B. burgdorferi sensu lato under 100% bootstrap support. This study opens up promising perspectives and reinforces the need for additional studies to determine the epidemiological characteristics of the disease, as well as the impact of the prevalence of Brazilian borreliosis in Mato Grosso do Sul State, Brazil. |
| abstract_unstemmed |
Abstract We aimed to detect DNA of Borrelia burgdorferi in whole blood and serum samples of patients with clinical symptoms and epidemiology compatible with Brazilian Lyme-like disease. Four patients with positive epidemiological histories were recruited for the study. Blood samples were collected, screened by serologic testing by ELISA and Western blotting and molecular identification of B. burgdorferi by amplifying a fragment of the conserved gene that synthesizes the hook flagellar flgE. The results showed positive serology and for the first time, the presence of B. burgdorferi sensu lato in humans in the Midwest region of Brazil. The resulting sequences were similar to GenBank corresponding sequences of B. burgdorferi flgE gene. By neighbor-joining the phylogenetic analysis, the flgE sequence of the Brazilian strain clustered in a monophyletic group with the sequence of B. burgdorferi sensu lato under 100% bootstrap support. This study opens up promising perspectives and reinforces the need for additional studies to determine the epidemiological characteristics of the disease, as well as the impact of the prevalence of Brazilian borreliosis in Mato Grosso do Sul State, Brazil. |
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GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ |
| title_short |
Molecular evidence of Borrelia burgdorferi sensu lato in patients in Brazilian central-western region |
| url |
https://doi.org/10.1016/j.rbre.2017.05.001 https://doaj.org/article/4672b52780d947b0a76c48973fd5fbb7 http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0482-50042017000600641&lng=en&tlng=en https://doaj.org/toc/1809-4570 |
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Jania de Rezende Danielly Beraldo dos Santos Silva Fernanda de Cássia Gonçalves Alves Carina Elisei de Oliveira Izaías Pereira da Costa |
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Jania de Rezende Danielly Beraldo dos Santos Silva Fernanda de Cássia Gonçalves Alves Carina Elisei de Oliveira Izaías Pereira da Costa |
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2024-07-03T13:46:50.886Z |
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