Determination of voriconazole and its N-oxide in human plasma by LC-MS/MS and its clinical application
Objective To develop liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the content determination of voriconazole (VRCZ) and its N-oxide (VNO) in human plasma, and to apply it to clinical samples. Methods Ketoconazole was used as the internal standard (IS). The plasma samples were treated...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
XIANG Rongfeng [verfasserIn] SHANG Shenglan [verfasserIn] XIONG Lirong [verfasserIn] YU Mingjie [verfasserIn] DAI Qing [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Chinesisch |
| Erschienen: |
2019 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
In: Di-san junyi daxue xuebao - Editorial Office of Journal of Third Military Medical University, 2021, 41(2019), 15, Seite 1478-1484 |
|---|---|
| Übergeordnetes Werk: |
volume:41 ; year:2019 ; number:15 ; pages:1478-1484 |
| Links: |
|---|
| DOI / URN: |
10.16016/j.1000-5404.201902067 |
|---|
| Katalog-ID: |
DOAJ077760611 |
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| 520 | |a Objective To develop liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the content determination of voriconazole (VRCZ) and its N-oxide (VNO) in human plasma, and to apply it to clinical samples. Methods Ketoconazole was used as the internal standard (IS). The plasma samples were treated with methanol to precipitate protein and analyzed by HPLC-MS/MS. The detection was accomplished with electrospray ionization (ESI) operated in positive ionization mode, and ion reaction pairs were selected to be m/z350.1→m/z127.0 (VRCZ), m/z366.1→m/z224.0 (VNO) and m/z531.0→m/z82.1 (Ketoconazole). Chromatographic separation had been achieved on Inertsil ODS-3 (50/2.1) with methanol-10 mmol/L ammonium acetate (90:10) as the mobile phase. Plasma concentrations of VRCZ and VNO in 23 patients were determined. Results Both VRCZ and VNO were linear (r<0.999) over the concentration ranges of 0.4~10.0 μg/mL, inter- and intra-batch relative standard deviations (RSDs) and matrix effect were all less than 15%. The 2 compounds remained stable during sample storage and handling progress. Plasma concentrations of VRCZ in 23 patients ranged from 0.4 to 14.3 μg/mL, and those of VNO ranged from 0.5 to 7.2 μg/mL. Significant variance was found among these patients. Conclusion LC-MS/MS is simple, quick and sensitive for detection of VRCZ and VNO, and can be used for drug monitoring in clinical practice. | ||
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| 650 | 4 | |a voriconazole n-oxide | |
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10.16016/j.1000-5404.201902067 doi (DE-627)DOAJ077760611 (DE-599)DOAJ843572511d9444fba3a10188ae596375 DE-627 ger DE-627 rakwb chi R5-920 XIANG Rongfeng verfasserin aut Determination of voriconazole and its N-oxide in human plasma by LC-MS/MS and its clinical application 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective To develop liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the content determination of voriconazole (VRCZ) and its N-oxide (VNO) in human plasma, and to apply it to clinical samples. Methods Ketoconazole was used as the internal standard (IS). The plasma samples were treated with methanol to precipitate protein and analyzed by HPLC-MS/MS. The detection was accomplished with electrospray ionization (ESI) operated in positive ionization mode, and ion reaction pairs were selected to be m/z350.1→m/z127.0 (VRCZ), m/z366.1→m/z224.0 (VNO) and m/z531.0→m/z82.1 (Ketoconazole). Chromatographic separation had been achieved on Inertsil ODS-3 (50/2.1) with methanol-10 mmol/L ammonium acetate (90:10) as the mobile phase. Plasma concentrations of VRCZ and VNO in 23 patients were determined. Results Both VRCZ and VNO were linear (r<0.999) over the concentration ranges of 0.4~10.0 μg/mL, inter- and intra-batch relative standard deviations (RSDs) and matrix effect were all less than 15%. The 2 compounds remained stable during sample storage and handling progress. Plasma concentrations of VRCZ in 23 patients ranged from 0.4 to 14.3 μg/mL, and those of VNO ranged from 0.5 to 7.2 μg/mL. Significant variance was found among these patients. Conclusion LC-MS/MS is simple, quick and sensitive for detection of VRCZ and VNO, and can be used for drug monitoring in clinical practice. voriconazole voriconazole n-oxide lc-ms/ms plasma concentration Medicine (General) SHANG Shenglan verfasserin aut XIONG Lirong verfasserin aut YU Mingjie verfasserin aut DAI Qing verfasserin aut In Di-san junyi daxue xuebao Editorial Office of Journal of Third Military Medical University, 2021 41(2019), 15, Seite 1478-1484 (DE-627)1760645346 10005404 nnns volume:41 year:2019 number:15 pages:1478-1484 https://doi.org/10.16016/j.1000-5404.201902067 kostenfrei https://doaj.org/article/843572511d9444fba3a10188ae596375 kostenfrei http://aammt.tmmu.edu.cn/Upload/rhtml/201902067.htm kostenfrei https://doaj.org/toc/1000-5404 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 41 2019 15 1478-1484 |
| spelling |
10.16016/j.1000-5404.201902067 doi (DE-627)DOAJ077760611 (DE-599)DOAJ843572511d9444fba3a10188ae596375 DE-627 ger DE-627 rakwb chi R5-920 XIANG Rongfeng verfasserin aut Determination of voriconazole and its N-oxide in human plasma by LC-MS/MS and its clinical application 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective To develop liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the content determination of voriconazole (VRCZ) and its N-oxide (VNO) in human plasma, and to apply it to clinical samples. Methods Ketoconazole was used as the internal standard (IS). The plasma samples were treated with methanol to precipitate protein and analyzed by HPLC-MS/MS. The detection was accomplished with electrospray ionization (ESI) operated in positive ionization mode, and ion reaction pairs were selected to be m/z350.1→m/z127.0 (VRCZ), m/z366.1→m/z224.0 (VNO) and m/z531.0→m/z82.1 (Ketoconazole). Chromatographic separation had been achieved on Inertsil ODS-3 (50/2.1) with methanol-10 mmol/L ammonium acetate (90:10) as the mobile phase. Plasma concentrations of VRCZ and VNO in 23 patients were determined. Results Both VRCZ and VNO were linear (r<0.999) over the concentration ranges of 0.4~10.0 μg/mL, inter- and intra-batch relative standard deviations (RSDs) and matrix effect were all less than 15%. The 2 compounds remained stable during sample storage and handling progress. Plasma concentrations of VRCZ in 23 patients ranged from 0.4 to 14.3 μg/mL, and those of VNO ranged from 0.5 to 7.2 μg/mL. Significant variance was found among these patients. Conclusion LC-MS/MS is simple, quick and sensitive for detection of VRCZ and VNO, and can be used for drug monitoring in clinical practice. voriconazole voriconazole n-oxide lc-ms/ms plasma concentration Medicine (General) SHANG Shenglan verfasserin aut XIONG Lirong verfasserin aut YU Mingjie verfasserin aut DAI Qing verfasserin aut In Di-san junyi daxue xuebao Editorial Office of Journal of Third Military Medical University, 2021 41(2019), 15, Seite 1478-1484 (DE-627)1760645346 10005404 nnns volume:41 year:2019 number:15 pages:1478-1484 https://doi.org/10.16016/j.1000-5404.201902067 kostenfrei https://doaj.org/article/843572511d9444fba3a10188ae596375 kostenfrei http://aammt.tmmu.edu.cn/Upload/rhtml/201902067.htm kostenfrei https://doaj.org/toc/1000-5404 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 41 2019 15 1478-1484 |
| allfields_unstemmed |
10.16016/j.1000-5404.201902067 doi (DE-627)DOAJ077760611 (DE-599)DOAJ843572511d9444fba3a10188ae596375 DE-627 ger DE-627 rakwb chi R5-920 XIANG Rongfeng verfasserin aut Determination of voriconazole and its N-oxide in human plasma by LC-MS/MS and its clinical application 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective To develop liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the content determination of voriconazole (VRCZ) and its N-oxide (VNO) in human plasma, and to apply it to clinical samples. Methods Ketoconazole was used as the internal standard (IS). The plasma samples were treated with methanol to precipitate protein and analyzed by HPLC-MS/MS. The detection was accomplished with electrospray ionization (ESI) operated in positive ionization mode, and ion reaction pairs were selected to be m/z350.1→m/z127.0 (VRCZ), m/z366.1→m/z224.0 (VNO) and m/z531.0→m/z82.1 (Ketoconazole). Chromatographic separation had been achieved on Inertsil ODS-3 (50/2.1) with methanol-10 mmol/L ammonium acetate (90:10) as the mobile phase. Plasma concentrations of VRCZ and VNO in 23 patients were determined. Results Both VRCZ and VNO were linear (r<0.999) over the concentration ranges of 0.4~10.0 μg/mL, inter- and intra-batch relative standard deviations (RSDs) and matrix effect were all less than 15%. The 2 compounds remained stable during sample storage and handling progress. Plasma concentrations of VRCZ in 23 patients ranged from 0.4 to 14.3 μg/mL, and those of VNO ranged from 0.5 to 7.2 μg/mL. Significant variance was found among these patients. Conclusion LC-MS/MS is simple, quick and sensitive for detection of VRCZ and VNO, and can be used for drug monitoring in clinical practice. voriconazole voriconazole n-oxide lc-ms/ms plasma concentration Medicine (General) SHANG Shenglan verfasserin aut XIONG Lirong verfasserin aut YU Mingjie verfasserin aut DAI Qing verfasserin aut In Di-san junyi daxue xuebao Editorial Office of Journal of Third Military Medical University, 2021 41(2019), 15, Seite 1478-1484 (DE-627)1760645346 10005404 nnns volume:41 year:2019 number:15 pages:1478-1484 https://doi.org/10.16016/j.1000-5404.201902067 kostenfrei https://doaj.org/article/843572511d9444fba3a10188ae596375 kostenfrei http://aammt.tmmu.edu.cn/Upload/rhtml/201902067.htm kostenfrei https://doaj.org/toc/1000-5404 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 41 2019 15 1478-1484 |
| allfieldsGer |
10.16016/j.1000-5404.201902067 doi (DE-627)DOAJ077760611 (DE-599)DOAJ843572511d9444fba3a10188ae596375 DE-627 ger DE-627 rakwb chi R5-920 XIANG Rongfeng verfasserin aut Determination of voriconazole and its N-oxide in human plasma by LC-MS/MS and its clinical application 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective To develop liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the content determination of voriconazole (VRCZ) and its N-oxide (VNO) in human plasma, and to apply it to clinical samples. Methods Ketoconazole was used as the internal standard (IS). The plasma samples were treated with methanol to precipitate protein and analyzed by HPLC-MS/MS. The detection was accomplished with electrospray ionization (ESI) operated in positive ionization mode, and ion reaction pairs were selected to be m/z350.1→m/z127.0 (VRCZ), m/z366.1→m/z224.0 (VNO) and m/z531.0→m/z82.1 (Ketoconazole). Chromatographic separation had been achieved on Inertsil ODS-3 (50/2.1) with methanol-10 mmol/L ammonium acetate (90:10) as the mobile phase. Plasma concentrations of VRCZ and VNO in 23 patients were determined. Results Both VRCZ and VNO were linear (r<0.999) over the concentration ranges of 0.4~10.0 μg/mL, inter- and intra-batch relative standard deviations (RSDs) and matrix effect were all less than 15%. The 2 compounds remained stable during sample storage and handling progress. Plasma concentrations of VRCZ in 23 patients ranged from 0.4 to 14.3 μg/mL, and those of VNO ranged from 0.5 to 7.2 μg/mL. Significant variance was found among these patients. Conclusion LC-MS/MS is simple, quick and sensitive for detection of VRCZ and VNO, and can be used for drug monitoring in clinical practice. voriconazole voriconazole n-oxide lc-ms/ms plasma concentration Medicine (General) SHANG Shenglan verfasserin aut XIONG Lirong verfasserin aut YU Mingjie verfasserin aut DAI Qing verfasserin aut In Di-san junyi daxue xuebao Editorial Office of Journal of Third Military Medical University, 2021 41(2019), 15, Seite 1478-1484 (DE-627)1760645346 10005404 nnns volume:41 year:2019 number:15 pages:1478-1484 https://doi.org/10.16016/j.1000-5404.201902067 kostenfrei https://doaj.org/article/843572511d9444fba3a10188ae596375 kostenfrei http://aammt.tmmu.edu.cn/Upload/rhtml/201902067.htm kostenfrei https://doaj.org/toc/1000-5404 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 41 2019 15 1478-1484 |
| allfieldsSound |
10.16016/j.1000-5404.201902067 doi (DE-627)DOAJ077760611 (DE-599)DOAJ843572511d9444fba3a10188ae596375 DE-627 ger DE-627 rakwb chi R5-920 XIANG Rongfeng verfasserin aut Determination of voriconazole and its N-oxide in human plasma by LC-MS/MS and its clinical application 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective To develop liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the content determination of voriconazole (VRCZ) and its N-oxide (VNO) in human plasma, and to apply it to clinical samples. Methods Ketoconazole was used as the internal standard (IS). The plasma samples were treated with methanol to precipitate protein and analyzed by HPLC-MS/MS. The detection was accomplished with electrospray ionization (ESI) operated in positive ionization mode, and ion reaction pairs were selected to be m/z350.1→m/z127.0 (VRCZ), m/z366.1→m/z224.0 (VNO) and m/z531.0→m/z82.1 (Ketoconazole). Chromatographic separation had been achieved on Inertsil ODS-3 (50/2.1) with methanol-10 mmol/L ammonium acetate (90:10) as the mobile phase. Plasma concentrations of VRCZ and VNO in 23 patients were determined. Results Both VRCZ and VNO were linear (r<0.999) over the concentration ranges of 0.4~10.0 μg/mL, inter- and intra-batch relative standard deviations (RSDs) and matrix effect were all less than 15%. The 2 compounds remained stable during sample storage and handling progress. Plasma concentrations of VRCZ in 23 patients ranged from 0.4 to 14.3 μg/mL, and those of VNO ranged from 0.5 to 7.2 μg/mL. Significant variance was found among these patients. Conclusion LC-MS/MS is simple, quick and sensitive for detection of VRCZ and VNO, and can be used for drug monitoring in clinical practice. voriconazole voriconazole n-oxide lc-ms/ms plasma concentration Medicine (General) SHANG Shenglan verfasserin aut XIONG Lirong verfasserin aut YU Mingjie verfasserin aut DAI Qing verfasserin aut In Di-san junyi daxue xuebao Editorial Office of Journal of Third Military Medical University, 2021 41(2019), 15, Seite 1478-1484 (DE-627)1760645346 10005404 nnns volume:41 year:2019 number:15 pages:1478-1484 https://doi.org/10.16016/j.1000-5404.201902067 kostenfrei https://doaj.org/article/843572511d9444fba3a10188ae596375 kostenfrei http://aammt.tmmu.edu.cn/Upload/rhtml/201902067.htm kostenfrei https://doaj.org/toc/1000-5404 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 41 2019 15 1478-1484 |
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XIANG Rongfeng misc R5-920 misc voriconazole misc voriconazole n-oxide misc lc-ms/ms misc plasma concentration misc Medicine (General) Determination of voriconazole and its N-oxide in human plasma by LC-MS/MS and its clinical application |
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R5-920 Determination of voriconazole and its N-oxide in human plasma by LC-MS/MS and its clinical application voriconazole voriconazole n-oxide lc-ms/ms plasma concentration |
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Determination of voriconazole and its N-oxide in human plasma by LC-MS/MS and its clinical application |
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Determination of voriconazole and its N-oxide in human plasma by LC-MS/MS and its clinical application |
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XIANG Rongfeng |
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Di-san junyi daxue xuebao |
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XIANG Rongfeng SHANG Shenglan XIONG Lirong YU Mingjie DAI Qing |
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10.16016/j.1000-5404.201902067 |
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determination of voriconazole and its n-oxide in human plasma by lc-ms/ms and its clinical application |
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Determination of voriconazole and its N-oxide in human plasma by LC-MS/MS and its clinical application |
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Objective To develop liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the content determination of voriconazole (VRCZ) and its N-oxide (VNO) in human plasma, and to apply it to clinical samples. Methods Ketoconazole was used as the internal standard (IS). The plasma samples were treated with methanol to precipitate protein and analyzed by HPLC-MS/MS. The detection was accomplished with electrospray ionization (ESI) operated in positive ionization mode, and ion reaction pairs were selected to be m/z350.1→m/z127.0 (VRCZ), m/z366.1→m/z224.0 (VNO) and m/z531.0→m/z82.1 (Ketoconazole). Chromatographic separation had been achieved on Inertsil ODS-3 (50/2.1) with methanol-10 mmol/L ammonium acetate (90:10) as the mobile phase. Plasma concentrations of VRCZ and VNO in 23 patients were determined. Results Both VRCZ and VNO were linear (r<0.999) over the concentration ranges of 0.4~10.0 μg/mL, inter- and intra-batch relative standard deviations (RSDs) and matrix effect were all less than 15%. The 2 compounds remained stable during sample storage and handling progress. Plasma concentrations of VRCZ in 23 patients ranged from 0.4 to 14.3 μg/mL, and those of VNO ranged from 0.5 to 7.2 μg/mL. Significant variance was found among these patients. Conclusion LC-MS/MS is simple, quick and sensitive for detection of VRCZ and VNO, and can be used for drug monitoring in clinical practice. |
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Objective To develop liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the content determination of voriconazole (VRCZ) and its N-oxide (VNO) in human plasma, and to apply it to clinical samples. Methods Ketoconazole was used as the internal standard (IS). The plasma samples were treated with methanol to precipitate protein and analyzed by HPLC-MS/MS. The detection was accomplished with electrospray ionization (ESI) operated in positive ionization mode, and ion reaction pairs were selected to be m/z350.1→m/z127.0 (VRCZ), m/z366.1→m/z224.0 (VNO) and m/z531.0→m/z82.1 (Ketoconazole). Chromatographic separation had been achieved on Inertsil ODS-3 (50/2.1) with methanol-10 mmol/L ammonium acetate (90:10) as the mobile phase. Plasma concentrations of VRCZ and VNO in 23 patients were determined. Results Both VRCZ and VNO were linear (r<0.999) over the concentration ranges of 0.4~10.0 μg/mL, inter- and intra-batch relative standard deviations (RSDs) and matrix effect were all less than 15%. The 2 compounds remained stable during sample storage and handling progress. Plasma concentrations of VRCZ in 23 patients ranged from 0.4 to 14.3 μg/mL, and those of VNO ranged from 0.5 to 7.2 μg/mL. Significant variance was found among these patients. Conclusion LC-MS/MS is simple, quick and sensitive for detection of VRCZ and VNO, and can be used for drug monitoring in clinical practice. |
| abstract_unstemmed |
Objective To develop liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the content determination of voriconazole (VRCZ) and its N-oxide (VNO) in human plasma, and to apply it to clinical samples. Methods Ketoconazole was used as the internal standard (IS). The plasma samples were treated with methanol to precipitate protein and analyzed by HPLC-MS/MS. The detection was accomplished with electrospray ionization (ESI) operated in positive ionization mode, and ion reaction pairs were selected to be m/z350.1→m/z127.0 (VRCZ), m/z366.1→m/z224.0 (VNO) and m/z531.0→m/z82.1 (Ketoconazole). Chromatographic separation had been achieved on Inertsil ODS-3 (50/2.1) with methanol-10 mmol/L ammonium acetate (90:10) as the mobile phase. Plasma concentrations of VRCZ and VNO in 23 patients were determined. Results Both VRCZ and VNO were linear (r<0.999) over the concentration ranges of 0.4~10.0 μg/mL, inter- and intra-batch relative standard deviations (RSDs) and matrix effect were all less than 15%. The 2 compounds remained stable during sample storage and handling progress. Plasma concentrations of VRCZ in 23 patients ranged from 0.4 to 14.3 μg/mL, and those of VNO ranged from 0.5 to 7.2 μg/mL. Significant variance was found among these patients. Conclusion LC-MS/MS is simple, quick and sensitive for detection of VRCZ and VNO, and can be used for drug monitoring in clinical practice. |
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Determination of voriconazole and its N-oxide in human plasma by LC-MS/MS and its clinical application |
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