Transcriptomic Prediction of Pig <i<Liver-Enriched Gene 1</i< Functions in a Liver Cell Line

The newly identified liver-enriched gene 1 (<i<LEG1</i<) encodes a protein with a characteristic domain of unknown function 781 (DUF781/LEG1), constituting a protein family with only one member in mammals. A functional study in zebrafish suggested that <i<LEG1</i< genes are i...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Zhe Zhang [verfasserIn] Zizengchen Wang [verfasserIn] Yanna Dang [verfasserIn] Jinyang Wang [verfasserIn] Sakthidasan Jayaprakash [verfasserIn] Huanan Wang [verfasserIn] Jin He [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	RNA-seq functional prediction pig

Übergeordnetes Werk:	In: Genes - MDPI AG, 2010, 11(2020), 4, p 412
Übergeordnetes Werk:	volume:11 ; year:2020 ; number:4, p 412

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/genes11040412

Katalog-ID:	DOAJ077768353

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520			\|a The newly identified liver-enriched gene 1 (<i<LEG1</i<) encodes a protein with a characteristic domain of unknown function 781 (DUF781/LEG1), constituting a protein family with only one member in mammals. A functional study in zebrafish suggested that <i<LEG1</i< genes are involved in liver development, while the platypus <i<LEG1</i< homolog, Monotreme Lactation Protein (<i<MLP</i<), which is enriched in the mammary gland and milk, acts as an antibacterial substance. However, no functional studies on eutherian <i<LEG1s</i< have been published to date. Thus, we here report the first functional prediction study at the cellular level. As previously reported, eutherian <i<LEG1s</i< can be classified into three paralogous groups. Pigs have all three <i<LEG1</i< genes (<i<pLEG1s</i<), while humans and mice have retained only <i<LEG1a</i<. Hence, <i<pLEG1s</i< might represent an ideal model for studying <i<LEG1</i< gene functions. RNA-seq was performed by the overexpression of <i<pLEG1s</i< and platypus <i<MLP</i< in HepG2 cells. Enrichment analysis showed that <i<pLEG1a</i< and <i<pLEG1b</i< might exhibit little function in liver cells; however, <i<pLEG1c</i< is probably involved in the endoplasmic reticulum (ER) stress response and protein folding. Additionally, gene set enrichment analysis revealed that platypus <i<MLP</i< shows antibacterial activity, confirming the functional study in platypus. Therefore, our study showed from the transcriptomic perspective that mammalian <i<LEG1s</i< have different functions in liver cells due to the subfunctionalization of paralogous genes.
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allfields	10.3390/genes11040412 doi (DE-627)DOAJ077768353 (DE-599)DOAJfab91e5548594a94b730f684f0351795 DE-627 ger DE-627 rakwb eng QH426-470 Zhe Zhang verfasserin aut Transcriptomic Prediction of Pig <i<Liver-Enriched Gene 1</i< Functions in a Liver Cell Line 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The newly identified liver-enriched gene 1 (<i<LEG1</i<) encodes a protein with a characteristic domain of unknown function 781 (DUF781/LEG1), constituting a protein family with only one member in mammals. A functional study in zebrafish suggested that <i<LEG1</i< genes are involved in liver development, while the platypus <i<LEG1</i< homolog, Monotreme Lactation Protein (<i<MLP</i<), which is enriched in the mammary gland and milk, acts as an antibacterial substance. However, no functional studies on eutherian <i<LEG1s</i< have been published to date. Thus, we here report the first functional prediction study at the cellular level. As previously reported, eutherian <i<LEG1s</i< can be classified into three paralogous groups. Pigs have all three <i<LEG1</i< genes (<i<pLEG1s</i<), while humans and mice have retained only <i<LEG1a</i<. Hence, <i<pLEG1s</i< might represent an ideal model for studying <i<LEG1</i< gene functions. RNA-seq was performed by the overexpression of <i<pLEG1s</i< and platypus <i<MLP</i< in HepG2 cells. Enrichment analysis showed that <i<pLEG1a</i< and <i<pLEG1b</i< might exhibit little function in liver cells; however, <i<pLEG1c</i< is probably involved in the endoplasmic reticulum (ER) stress response and protein folding. Additionally, gene set enrichment analysis revealed that platypus <i<MLP</i< shows antibacterial activity, confirming the functional study in platypus. Therefore, our study showed from the transcriptomic perspective that mammalian <i<LEG1s</i< have different functions in liver cells due to the subfunctionalization of paralogous genes. <i<LEG1</i< RNA-seq functional prediction pig Genetics Zizengchen Wang verfasserin aut Yanna Dang verfasserin aut Jinyang Wang verfasserin aut Sakthidasan Jayaprakash verfasserin aut Huanan Wang verfasserin aut Jin He verfasserin aut In Genes MDPI AG, 2010 11(2020), 4, p 412 (DE-627)614096537 (DE-600)2527218-4 20734425 nnns volume:11 year:2020 number:4, p 412 https://doi.org/10.3390/genes11040412 kostenfrei https://doaj.org/article/fab91e5548594a94b730f684f0351795 kostenfrei https://www.mdpi.com/2073-4425/11/4/412 kostenfrei https://doaj.org/toc/2073-4425 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2020 4, p 412
spelling	10.3390/genes11040412 doi (DE-627)DOAJ077768353 (DE-599)DOAJfab91e5548594a94b730f684f0351795 DE-627 ger DE-627 rakwb eng QH426-470 Zhe Zhang verfasserin aut Transcriptomic Prediction of Pig <i<Liver-Enriched Gene 1</i< Functions in a Liver Cell Line 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The newly identified liver-enriched gene 1 (<i<LEG1</i<) encodes a protein with a characteristic domain of unknown function 781 (DUF781/LEG1), constituting a protein family with only one member in mammals. A functional study in zebrafish suggested that <i<LEG1</i< genes are involved in liver development, while the platypus <i<LEG1</i< homolog, Monotreme Lactation Protein (<i<MLP</i<), which is enriched in the mammary gland and milk, acts as an antibacterial substance. However, no functional studies on eutherian <i<LEG1s</i< have been published to date. Thus, we here report the first functional prediction study at the cellular level. As previously reported, eutherian <i<LEG1s</i< can be classified into three paralogous groups. Pigs have all three <i<LEG1</i< genes (<i<pLEG1s</i<), while humans and mice have retained only <i<LEG1a</i<. Hence, <i<pLEG1s</i< might represent an ideal model for studying <i<LEG1</i< gene functions. RNA-seq was performed by the overexpression of <i<pLEG1s</i< and platypus <i<MLP</i< in HepG2 cells. Enrichment analysis showed that <i<pLEG1a</i< and <i<pLEG1b</i< might exhibit little function in liver cells; however, <i<pLEG1c</i< is probably involved in the endoplasmic reticulum (ER) stress response and protein folding. Additionally, gene set enrichment analysis revealed that platypus <i<MLP</i< shows antibacterial activity, confirming the functional study in platypus. Therefore, our study showed from the transcriptomic perspective that mammalian <i<LEG1s</i< have different functions in liver cells due to the subfunctionalization of paralogous genes. <i<LEG1</i< RNA-seq functional prediction pig Genetics Zizengchen Wang verfasserin aut Yanna Dang verfasserin aut Jinyang Wang verfasserin aut Sakthidasan Jayaprakash verfasserin aut Huanan Wang verfasserin aut Jin He verfasserin aut In Genes MDPI AG, 2010 11(2020), 4, p 412 (DE-627)614096537 (DE-600)2527218-4 20734425 nnns volume:11 year:2020 number:4, p 412 https://doi.org/10.3390/genes11040412 kostenfrei https://doaj.org/article/fab91e5548594a94b730f684f0351795 kostenfrei https://www.mdpi.com/2073-4425/11/4/412 kostenfrei https://doaj.org/toc/2073-4425 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2020 4, p 412
allfields_unstemmed	10.3390/genes11040412 doi (DE-627)DOAJ077768353 (DE-599)DOAJfab91e5548594a94b730f684f0351795 DE-627 ger DE-627 rakwb eng QH426-470 Zhe Zhang verfasserin aut Transcriptomic Prediction of Pig <i<Liver-Enriched Gene 1</i< Functions in a Liver Cell Line 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The newly identified liver-enriched gene 1 (<i<LEG1</i<) encodes a protein with a characteristic domain of unknown function 781 (DUF781/LEG1), constituting a protein family with only one member in mammals. A functional study in zebrafish suggested that <i<LEG1</i< genes are involved in liver development, while the platypus <i<LEG1</i< homolog, Monotreme Lactation Protein (<i<MLP</i<), which is enriched in the mammary gland and milk, acts as an antibacterial substance. However, no functional studies on eutherian <i<LEG1s</i< have been published to date. Thus, we here report the first functional prediction study at the cellular level. As previously reported, eutherian <i<LEG1s</i< can be classified into three paralogous groups. Pigs have all three <i<LEG1</i< genes (<i<pLEG1s</i<), while humans and mice have retained only <i<LEG1a</i<. Hence, <i<pLEG1s</i< might represent an ideal model for studying <i<LEG1</i< gene functions. RNA-seq was performed by the overexpression of <i<pLEG1s</i< and platypus <i<MLP</i< in HepG2 cells. Enrichment analysis showed that <i<pLEG1a</i< and <i<pLEG1b</i< might exhibit little function in liver cells; however, <i<pLEG1c</i< is probably involved in the endoplasmic reticulum (ER) stress response and protein folding. Additionally, gene set enrichment analysis revealed that platypus <i<MLP</i< shows antibacterial activity, confirming the functional study in platypus. Therefore, our study showed from the transcriptomic perspective that mammalian <i<LEG1s</i< have different functions in liver cells due to the subfunctionalization of paralogous genes. <i<LEG1</i< RNA-seq functional prediction pig Genetics Zizengchen Wang verfasserin aut Yanna Dang verfasserin aut Jinyang Wang verfasserin aut Sakthidasan Jayaprakash verfasserin aut Huanan Wang verfasserin aut Jin He verfasserin aut In Genes MDPI AG, 2010 11(2020), 4, p 412 (DE-627)614096537 (DE-600)2527218-4 20734425 nnns volume:11 year:2020 number:4, p 412 https://doi.org/10.3390/genes11040412 kostenfrei https://doaj.org/article/fab91e5548594a94b730f684f0351795 kostenfrei https://www.mdpi.com/2073-4425/11/4/412 kostenfrei https://doaj.org/toc/2073-4425 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2020 4, p 412
allfieldsGer	10.3390/genes11040412 doi (DE-627)DOAJ077768353 (DE-599)DOAJfab91e5548594a94b730f684f0351795 DE-627 ger DE-627 rakwb eng QH426-470 Zhe Zhang verfasserin aut Transcriptomic Prediction of Pig <i<Liver-Enriched Gene 1</i< Functions in a Liver Cell Line 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The newly identified liver-enriched gene 1 (<i<LEG1</i<) encodes a protein with a characteristic domain of unknown function 781 (DUF781/LEG1), constituting a protein family with only one member in mammals. A functional study in zebrafish suggested that <i<LEG1</i< genes are involved in liver development, while the platypus <i<LEG1</i< homolog, Monotreme Lactation Protein (<i<MLP</i<), which is enriched in the mammary gland and milk, acts as an antibacterial substance. However, no functional studies on eutherian <i<LEG1s</i< have been published to date. Thus, we here report the first functional prediction study at the cellular level. As previously reported, eutherian <i<LEG1s</i< can be classified into three paralogous groups. Pigs have all three <i<LEG1</i< genes (<i<pLEG1s</i<), while humans and mice have retained only <i<LEG1a</i<. Hence, <i<pLEG1s</i< might represent an ideal model for studying <i<LEG1</i< gene functions. RNA-seq was performed by the overexpression of <i<pLEG1s</i< and platypus <i<MLP</i< in HepG2 cells. Enrichment analysis showed that <i<pLEG1a</i< and <i<pLEG1b</i< might exhibit little function in liver cells; however, <i<pLEG1c</i< is probably involved in the endoplasmic reticulum (ER) stress response and protein folding. Additionally, gene set enrichment analysis revealed that platypus <i<MLP</i< shows antibacterial activity, confirming the functional study in platypus. Therefore, our study showed from the transcriptomic perspective that mammalian <i<LEG1s</i< have different functions in liver cells due to the subfunctionalization of paralogous genes. <i<LEG1</i< RNA-seq functional prediction pig Genetics Zizengchen Wang verfasserin aut Yanna Dang verfasserin aut Jinyang Wang verfasserin aut Sakthidasan Jayaprakash verfasserin aut Huanan Wang verfasserin aut Jin He verfasserin aut In Genes MDPI AG, 2010 11(2020), 4, p 412 (DE-627)614096537 (DE-600)2527218-4 20734425 nnns volume:11 year:2020 number:4, p 412 https://doi.org/10.3390/genes11040412 kostenfrei https://doaj.org/article/fab91e5548594a94b730f684f0351795 kostenfrei https://www.mdpi.com/2073-4425/11/4/412 kostenfrei https://doaj.org/toc/2073-4425 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2020 4, p 412
allfieldsSound	10.3390/genes11040412 doi (DE-627)DOAJ077768353 (DE-599)DOAJfab91e5548594a94b730f684f0351795 DE-627 ger DE-627 rakwb eng QH426-470 Zhe Zhang verfasserin aut Transcriptomic Prediction of Pig <i<Liver-Enriched Gene 1</i< Functions in a Liver Cell Line 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The newly identified liver-enriched gene 1 (<i<LEG1</i<) encodes a protein with a characteristic domain of unknown function 781 (DUF781/LEG1), constituting a protein family with only one member in mammals. A functional study in zebrafish suggested that <i<LEG1</i< genes are involved in liver development, while the platypus <i<LEG1</i< homolog, Monotreme Lactation Protein (<i<MLP</i<), which is enriched in the mammary gland and milk, acts as an antibacterial substance. However, no functional studies on eutherian <i<LEG1s</i< have been published to date. Thus, we here report the first functional prediction study at the cellular level. As previously reported, eutherian <i<LEG1s</i< can be classified into three paralogous groups. Pigs have all three <i<LEG1</i< genes (<i<pLEG1s</i<), while humans and mice have retained only <i<LEG1a</i<. Hence, <i<pLEG1s</i< might represent an ideal model for studying <i<LEG1</i< gene functions. RNA-seq was performed by the overexpression of <i<pLEG1s</i< and platypus <i<MLP</i< in HepG2 cells. Enrichment analysis showed that <i<pLEG1a</i< and <i<pLEG1b</i< might exhibit little function in liver cells; however, <i<pLEG1c</i< is probably involved in the endoplasmic reticulum (ER) stress response and protein folding. Additionally, gene set enrichment analysis revealed that platypus <i<MLP</i< shows antibacterial activity, confirming the functional study in platypus. Therefore, our study showed from the transcriptomic perspective that mammalian <i<LEG1s</i< have different functions in liver cells due to the subfunctionalization of paralogous genes. <i<LEG1</i< RNA-seq functional prediction pig Genetics Zizengchen Wang verfasserin aut Yanna Dang verfasserin aut Jinyang Wang verfasserin aut Sakthidasan Jayaprakash verfasserin aut Huanan Wang verfasserin aut Jin He verfasserin aut In Genes MDPI AG, 2010 11(2020), 4, p 412 (DE-627)614096537 (DE-600)2527218-4 20734425 nnns volume:11 year:2020 number:4, p 412 https://doi.org/10.3390/genes11040412 kostenfrei https://doaj.org/article/fab91e5548594a94b730f684f0351795 kostenfrei https://www.mdpi.com/2073-4425/11/4/412 kostenfrei https://doaj.org/toc/2073-4425 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2020 4, p 412
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authorswithroles_txt_mv	Zhe Zhang @@aut@@ Zizengchen Wang @@aut@@ Yanna Dang @@aut@@ Jinyang Wang @@aut@@ Sakthidasan Jayaprakash @@aut@@ Huanan Wang @@aut@@ Jin He @@aut@@
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callnumber-first	Q - Science
author	Zhe Zhang
spellingShingle	Zhe Zhang misc QH426-470 misc <i<LEG1</i< misc RNA-seq misc functional prediction misc pig misc Genetics Transcriptomic Prediction of Pig <i<Liver-Enriched Gene 1</i< Functions in a Liver Cell Line
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topic_title	QH426-470 Transcriptomic Prediction of Pig <i<Liver-Enriched Gene 1</i< Functions in a Liver Cell Line <i<LEG1</i< RNA-seq functional prediction pig
topic	misc QH426-470 misc <i<LEG1</i< misc RNA-seq misc functional prediction misc pig misc Genetics
topic_unstemmed	misc QH426-470 misc <i<LEG1</i< misc RNA-seq misc functional prediction misc pig misc Genetics
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title	Transcriptomic Prediction of Pig <i<Liver-Enriched Gene 1</i< Functions in a Liver Cell Line
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title_full	Transcriptomic Prediction of Pig <i<Liver-Enriched Gene 1</i< Functions in a Liver Cell Line
author_sort	Zhe Zhang
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author_browse	Zhe Zhang Zizengchen Wang Yanna Dang Jinyang Wang Sakthidasan Jayaprakash Huanan Wang Jin He
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title_sort	transcriptomic prediction of pig <i<liver-enriched gene 1</i< functions in a liver cell line
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title_auth	Transcriptomic Prediction of Pig <i<Liver-Enriched Gene 1</i< Functions in a Liver Cell Line
abstract	The newly identified liver-enriched gene 1 (<i<LEG1</i<) encodes a protein with a characteristic domain of unknown function 781 (DUF781/LEG1), constituting a protein family with only one member in mammals. A functional study in zebrafish suggested that <i<LEG1</i< genes are involved in liver development, while the platypus <i<LEG1</i< homolog, Monotreme Lactation Protein (<i<MLP</i<), which is enriched in the mammary gland and milk, acts as an antibacterial substance. However, no functional studies on eutherian <i<LEG1s</i< have been published to date. Thus, we here report the first functional prediction study at the cellular level. As previously reported, eutherian <i<LEG1s</i< can be classified into three paralogous groups. Pigs have all three <i<LEG1</i< genes (<i<pLEG1s</i<), while humans and mice have retained only <i<LEG1a</i<. Hence, <i<pLEG1s</i< might represent an ideal model for studying <i<LEG1</i< gene functions. RNA-seq was performed by the overexpression of <i<pLEG1s</i< and platypus <i<MLP</i< in HepG2 cells. Enrichment analysis showed that <i<pLEG1a</i< and <i<pLEG1b</i< might exhibit little function in liver cells; however, <i<pLEG1c</i< is probably involved in the endoplasmic reticulum (ER) stress response and protein folding. Additionally, gene set enrichment analysis revealed that platypus <i<MLP</i< shows antibacterial activity, confirming the functional study in platypus. Therefore, our study showed from the transcriptomic perspective that mammalian <i<LEG1s</i< have different functions in liver cells due to the subfunctionalization of paralogous genes.
abstractGer	The newly identified liver-enriched gene 1 (<i<LEG1</i<) encodes a protein with a characteristic domain of unknown function 781 (DUF781/LEG1), constituting a protein family with only one member in mammals. A functional study in zebrafish suggested that <i<LEG1</i< genes are involved in liver development, while the platypus <i<LEG1</i< homolog, Monotreme Lactation Protein (<i<MLP</i<), which is enriched in the mammary gland and milk, acts as an antibacterial substance. However, no functional studies on eutherian <i<LEG1s</i< have been published to date. Thus, we here report the first functional prediction study at the cellular level. As previously reported, eutherian <i<LEG1s</i< can be classified into three paralogous groups. Pigs have all three <i<LEG1</i< genes (<i<pLEG1s</i<), while humans and mice have retained only <i<LEG1a</i<. Hence, <i<pLEG1s</i< might represent an ideal model for studying <i<LEG1</i< gene functions. RNA-seq was performed by the overexpression of <i<pLEG1s</i< and platypus <i<MLP</i< in HepG2 cells. Enrichment analysis showed that <i<pLEG1a</i< and <i<pLEG1b</i< might exhibit little function in liver cells; however, <i<pLEG1c</i< is probably involved in the endoplasmic reticulum (ER) stress response and protein folding. Additionally, gene set enrichment analysis revealed that platypus <i<MLP</i< shows antibacterial activity, confirming the functional study in platypus. Therefore, our study showed from the transcriptomic perspective that mammalian <i<LEG1s</i< have different functions in liver cells due to the subfunctionalization of paralogous genes.
abstract_unstemmed	The newly identified liver-enriched gene 1 (<i<LEG1</i<) encodes a protein with a characteristic domain of unknown function 781 (DUF781/LEG1), constituting a protein family with only one member in mammals. A functional study in zebrafish suggested that <i<LEG1</i< genes are involved in liver development, while the platypus <i<LEG1</i< homolog, Monotreme Lactation Protein (<i<MLP</i<), which is enriched in the mammary gland and milk, acts as an antibacterial substance. However, no functional studies on eutherian <i<LEG1s</i< have been published to date. Thus, we here report the first functional prediction study at the cellular level. As previously reported, eutherian <i<LEG1s</i< can be classified into three paralogous groups. Pigs have all three <i<LEG1</i< genes (<i<pLEG1s</i<), while humans and mice have retained only <i<LEG1a</i<. Hence, <i<pLEG1s</i< might represent an ideal model for studying <i<LEG1</i< gene functions. RNA-seq was performed by the overexpression of <i<pLEG1s</i< and platypus <i<MLP</i< in HepG2 cells. Enrichment analysis showed that <i<pLEG1a</i< and <i<pLEG1b</i< might exhibit little function in liver cells; however, <i<pLEG1c</i< is probably involved in the endoplasmic reticulum (ER) stress response and protein folding. Additionally, gene set enrichment analysis revealed that platypus <i<MLP</i< shows antibacterial activity, confirming the functional study in platypus. Therefore, our study showed from the transcriptomic perspective that mammalian <i<LEG1s</i< have different functions in liver cells due to the subfunctionalization of paralogous genes.
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title_short	Transcriptomic Prediction of Pig <i<Liver-Enriched Gene 1</i< Functions in a Liver Cell Line
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up_date	2024-07-03T13:59:38.112Z
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Enrichment analysis showed that <i<pLEG1a</i< and <i<pLEG1b</i< might exhibit little function in liver cells; however, <i<pLEG1c</i< is probably involved in the endoplasmic reticulum (ER) stress response and protein folding. Additionally, gene set enrichment analysis revealed that platypus <i<MLP</i< shows antibacterial activity, confirming the functional study in platypus. 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Transcriptomic Prediction of Pig <i<Liver-Enriched Gene 1</i< Functions in a Liver Cell Line

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