Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix

Decellularized cardiac extracellular matrix scaffolds with preserved composition and architecture can be used in tissue engineering to reproduce the complex cardiac extracellular matrix. However, evaluating the extent of cardiomyocyte repopulation of decellularized cardiac extracellular matrix scaff...
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Autor*in:	Camila Hochman-Mendez [verfasserIn] Dilza Balteiro Pereira de Campos [verfasserIn] Rafael Serafim Pinto [verfasserIn] Bernardo Jorge da Silva Mendes [verfasserIn] Gustavo Miranda Rocha [verfasserIn] Gustavo Monnerat [verfasserIn] Gilberto Weissmuller [verfasserIn] Luiz C Sampaio [verfasserIn] Adriana Bastos Carvalho [verfasserIn] Doris A Taylor [verfasserIn] Antonio Carlos Campos de Carvalho [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Übergeordnetes Werk:	In: Journal of Tissue Engineering - SAGE Publishing, 2010, 11(2020)
Übergeordnetes Werk:	volume:11 ; year:2020

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1177/2041731420921482

Katalog-ID:	DOAJ077817311

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allfields	10.1177/2041731420921482 doi (DE-627)DOAJ077817311 (DE-599)DOAJ7cfe3cd8bcee454ca01755a9a9aa1c17 DE-627 ger DE-627 rakwb eng QD415-436 Camila Hochman-Mendez verfasserin aut Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Decellularized cardiac extracellular matrix scaffolds with preserved composition and architecture can be used in tissue engineering to reproduce the complex cardiac extracellular matrix. However, evaluating the extent of cardiomyocyte repopulation of decellularized cardiac extracellular matrix scaffolds after recellularization attempts is challenging. Here, we describe a unique combination of biochemical, biomechanical, histological, and physiological parameters for quantifying recellularization efficiency of tissue-engineered cardiac patches compared with native cardiac tissue. Human embryonic stem cell-derived cardiomyocytes were seeded into rat heart atrial and ventricular decellularized cardiac extracellular matrix patches. Confocal and atomic force microscopy showed cell integration within the extracellular matrix basement membrane that was accompanied by restoration of native cardiac tissue passive mechanical properties. Multi-electrode array and immunostaining (connexin 43) were used to determine synchronous field potentials with electrical coupling. Myoglobin content (~60%) and sarcomere length measurement (<45% vs 2D culture) were used to evaluate cardiomyocyte maturation of integrated cells. The combination of these techniques allowed us to demonstrate that as cellularization efficiency improves, cardiomyocytes mature and synchronize electrical activity, and tissue mechanical/biochemical properties improve toward those of native tissue. Biochemistry Dilza Balteiro Pereira de Campos verfasserin aut Rafael Serafim Pinto verfasserin aut Bernardo Jorge da Silva Mendes verfasserin aut Gustavo Miranda Rocha verfasserin aut Gustavo Monnerat verfasserin aut Gilberto Weissmuller verfasserin aut Luiz C Sampaio verfasserin aut Adriana Bastos Carvalho verfasserin aut Doris A Taylor verfasserin aut Antonio Carlos Campos de Carvalho verfasserin aut In Journal of Tissue Engineering SAGE Publishing, 2010 11(2020) (DE-627)635606240 (DE-600)2573915-3 20417314 nnns volume:11 year:2020 https://doi.org/10.1177/2041731420921482 kostenfrei https://doaj.org/article/7cfe3cd8bcee454ca01755a9a9aa1c17 kostenfrei https://doi.org/10.1177/2041731420921482 kostenfrei https://doaj.org/toc/2041-7314 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_374 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2111 GBV_ILN_2704 GBV_ILN_2707 GBV_ILN_2889 GBV_ILN_2890 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2020
spelling	10.1177/2041731420921482 doi (DE-627)DOAJ077817311 (DE-599)DOAJ7cfe3cd8bcee454ca01755a9a9aa1c17 DE-627 ger DE-627 rakwb eng QD415-436 Camila Hochman-Mendez verfasserin aut Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Decellularized cardiac extracellular matrix scaffolds with preserved composition and architecture can be used in tissue engineering to reproduce the complex cardiac extracellular matrix. However, evaluating the extent of cardiomyocyte repopulation of decellularized cardiac extracellular matrix scaffolds after recellularization attempts is challenging. Here, we describe a unique combination of biochemical, biomechanical, histological, and physiological parameters for quantifying recellularization efficiency of tissue-engineered cardiac patches compared with native cardiac tissue. Human embryonic stem cell-derived cardiomyocytes were seeded into rat heart atrial and ventricular decellularized cardiac extracellular matrix patches. Confocal and atomic force microscopy showed cell integration within the extracellular matrix basement membrane that was accompanied by restoration of native cardiac tissue passive mechanical properties. Multi-electrode array and immunostaining (connexin 43) were used to determine synchronous field potentials with electrical coupling. Myoglobin content (~60%) and sarcomere length measurement (<45% vs 2D culture) were used to evaluate cardiomyocyte maturation of integrated cells. The combination of these techniques allowed us to demonstrate that as cellularization efficiency improves, cardiomyocytes mature and synchronize electrical activity, and tissue mechanical/biochemical properties improve toward those of native tissue. Biochemistry Dilza Balteiro Pereira de Campos verfasserin aut Rafael Serafim Pinto verfasserin aut Bernardo Jorge da Silva Mendes verfasserin aut Gustavo Miranda Rocha verfasserin aut Gustavo Monnerat verfasserin aut Gilberto Weissmuller verfasserin aut Luiz C Sampaio verfasserin aut Adriana Bastos Carvalho verfasserin aut Doris A Taylor verfasserin aut Antonio Carlos Campos de Carvalho verfasserin aut In Journal of Tissue Engineering SAGE Publishing, 2010 11(2020) (DE-627)635606240 (DE-600)2573915-3 20417314 nnns volume:11 year:2020 https://doi.org/10.1177/2041731420921482 kostenfrei https://doaj.org/article/7cfe3cd8bcee454ca01755a9a9aa1c17 kostenfrei https://doi.org/10.1177/2041731420921482 kostenfrei https://doaj.org/toc/2041-7314 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_374 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2111 GBV_ILN_2704 GBV_ILN_2707 GBV_ILN_2889 GBV_ILN_2890 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2020
allfields_unstemmed	10.1177/2041731420921482 doi (DE-627)DOAJ077817311 (DE-599)DOAJ7cfe3cd8bcee454ca01755a9a9aa1c17 DE-627 ger DE-627 rakwb eng QD415-436 Camila Hochman-Mendez verfasserin aut Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Decellularized cardiac extracellular matrix scaffolds with preserved composition and architecture can be used in tissue engineering to reproduce the complex cardiac extracellular matrix. However, evaluating the extent of cardiomyocyte repopulation of decellularized cardiac extracellular matrix scaffolds after recellularization attempts is challenging. Here, we describe a unique combination of biochemical, biomechanical, histological, and physiological parameters for quantifying recellularization efficiency of tissue-engineered cardiac patches compared with native cardiac tissue. Human embryonic stem cell-derived cardiomyocytes were seeded into rat heart atrial and ventricular decellularized cardiac extracellular matrix patches. Confocal and atomic force microscopy showed cell integration within the extracellular matrix basement membrane that was accompanied by restoration of native cardiac tissue passive mechanical properties. Multi-electrode array and immunostaining (connexin 43) were used to determine synchronous field potentials with electrical coupling. Myoglobin content (~60%) and sarcomere length measurement (<45% vs 2D culture) were used to evaluate cardiomyocyte maturation of integrated cells. The combination of these techniques allowed us to demonstrate that as cellularization efficiency improves, cardiomyocytes mature and synchronize electrical activity, and tissue mechanical/biochemical properties improve toward those of native tissue. Biochemistry Dilza Balteiro Pereira de Campos verfasserin aut Rafael Serafim Pinto verfasserin aut Bernardo Jorge da Silva Mendes verfasserin aut Gustavo Miranda Rocha verfasserin aut Gustavo Monnerat verfasserin aut Gilberto Weissmuller verfasserin aut Luiz C Sampaio verfasserin aut Adriana Bastos Carvalho verfasserin aut Doris A Taylor verfasserin aut Antonio Carlos Campos de Carvalho verfasserin aut In Journal of Tissue Engineering SAGE Publishing, 2010 11(2020) (DE-627)635606240 (DE-600)2573915-3 20417314 nnns volume:11 year:2020 https://doi.org/10.1177/2041731420921482 kostenfrei https://doaj.org/article/7cfe3cd8bcee454ca01755a9a9aa1c17 kostenfrei https://doi.org/10.1177/2041731420921482 kostenfrei https://doaj.org/toc/2041-7314 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_374 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2111 GBV_ILN_2704 GBV_ILN_2707 GBV_ILN_2889 GBV_ILN_2890 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2020
allfieldsGer	10.1177/2041731420921482 doi (DE-627)DOAJ077817311 (DE-599)DOAJ7cfe3cd8bcee454ca01755a9a9aa1c17 DE-627 ger DE-627 rakwb eng QD415-436 Camila Hochman-Mendez verfasserin aut Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Decellularized cardiac extracellular matrix scaffolds with preserved composition and architecture can be used in tissue engineering to reproduce the complex cardiac extracellular matrix. However, evaluating the extent of cardiomyocyte repopulation of decellularized cardiac extracellular matrix scaffolds after recellularization attempts is challenging. Here, we describe a unique combination of biochemical, biomechanical, histological, and physiological parameters for quantifying recellularization efficiency of tissue-engineered cardiac patches compared with native cardiac tissue. Human embryonic stem cell-derived cardiomyocytes were seeded into rat heart atrial and ventricular decellularized cardiac extracellular matrix patches. Confocal and atomic force microscopy showed cell integration within the extracellular matrix basement membrane that was accompanied by restoration of native cardiac tissue passive mechanical properties. Multi-electrode array and immunostaining (connexin 43) were used to determine synchronous field potentials with electrical coupling. Myoglobin content (~60%) and sarcomere length measurement (<45% vs 2D culture) were used to evaluate cardiomyocyte maturation of integrated cells. The combination of these techniques allowed us to demonstrate that as cellularization efficiency improves, cardiomyocytes mature and synchronize electrical activity, and tissue mechanical/biochemical properties improve toward those of native tissue. Biochemistry Dilza Balteiro Pereira de Campos verfasserin aut Rafael Serafim Pinto verfasserin aut Bernardo Jorge da Silva Mendes verfasserin aut Gustavo Miranda Rocha verfasserin aut Gustavo Monnerat verfasserin aut Gilberto Weissmuller verfasserin aut Luiz C Sampaio verfasserin aut Adriana Bastos Carvalho verfasserin aut Doris A Taylor verfasserin aut Antonio Carlos Campos de Carvalho verfasserin aut In Journal of Tissue Engineering SAGE Publishing, 2010 11(2020) (DE-627)635606240 (DE-600)2573915-3 20417314 nnns volume:11 year:2020 https://doi.org/10.1177/2041731420921482 kostenfrei https://doaj.org/article/7cfe3cd8bcee454ca01755a9a9aa1c17 kostenfrei https://doi.org/10.1177/2041731420921482 kostenfrei https://doaj.org/toc/2041-7314 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_374 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2111 GBV_ILN_2704 GBV_ILN_2707 GBV_ILN_2889 GBV_ILN_2890 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2020
allfieldsSound	10.1177/2041731420921482 doi (DE-627)DOAJ077817311 (DE-599)DOAJ7cfe3cd8bcee454ca01755a9a9aa1c17 DE-627 ger DE-627 rakwb eng QD415-436 Camila Hochman-Mendez verfasserin aut Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Decellularized cardiac extracellular matrix scaffolds with preserved composition and architecture can be used in tissue engineering to reproduce the complex cardiac extracellular matrix. However, evaluating the extent of cardiomyocyte repopulation of decellularized cardiac extracellular matrix scaffolds after recellularization attempts is challenging. Here, we describe a unique combination of biochemical, biomechanical, histological, and physiological parameters for quantifying recellularization efficiency of tissue-engineered cardiac patches compared with native cardiac tissue. Human embryonic stem cell-derived cardiomyocytes were seeded into rat heart atrial and ventricular decellularized cardiac extracellular matrix patches. Confocal and atomic force microscopy showed cell integration within the extracellular matrix basement membrane that was accompanied by restoration of native cardiac tissue passive mechanical properties. Multi-electrode array and immunostaining (connexin 43) were used to determine synchronous field potentials with electrical coupling. Myoglobin content (~60%) and sarcomere length measurement (<45% vs 2D culture) were used to evaluate cardiomyocyte maturation of integrated cells. The combination of these techniques allowed us to demonstrate that as cellularization efficiency improves, cardiomyocytes mature and synchronize electrical activity, and tissue mechanical/biochemical properties improve toward those of native tissue. Biochemistry Dilza Balteiro Pereira de Campos verfasserin aut Rafael Serafim Pinto verfasserin aut Bernardo Jorge da Silva Mendes verfasserin aut Gustavo Miranda Rocha verfasserin aut Gustavo Monnerat verfasserin aut Gilberto Weissmuller verfasserin aut Luiz C Sampaio verfasserin aut Adriana Bastos Carvalho verfasserin aut Doris A Taylor verfasserin aut Antonio Carlos Campos de Carvalho verfasserin aut In Journal of Tissue Engineering SAGE Publishing, 2010 11(2020) (DE-627)635606240 (DE-600)2573915-3 20417314 nnns volume:11 year:2020 https://doi.org/10.1177/2041731420921482 kostenfrei https://doaj.org/article/7cfe3cd8bcee454ca01755a9a9aa1c17 kostenfrei https://doi.org/10.1177/2041731420921482 kostenfrei https://doaj.org/toc/2041-7314 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_374 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2111 GBV_ILN_2704 GBV_ILN_2707 GBV_ILN_2889 GBV_ILN_2890 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2020
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authorswithroles_txt_mv	Camila Hochman-Mendez @@aut@@ Dilza Balteiro Pereira de Campos @@aut@@ Rafael Serafim Pinto @@aut@@ Bernardo Jorge da Silva Mendes @@aut@@ Gustavo Miranda Rocha @@aut@@ Gustavo Monnerat @@aut@@ Gilberto Weissmuller @@aut@@ Luiz C Sampaio @@aut@@ Adriana Bastos Carvalho @@aut@@ Doris A Taylor @@aut@@ Antonio Carlos Campos de Carvalho @@aut@@
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author	Camila Hochman-Mendez
spellingShingle	Camila Hochman-Mendez misc QD415-436 misc Biochemistry Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix
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topic_title	QD415-436 Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix
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title	Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix
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title_full	Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix
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author_browse	Camila Hochman-Mendez Dilza Balteiro Pereira de Campos Rafael Serafim Pinto Bernardo Jorge da Silva Mendes Gustavo Miranda Rocha Gustavo Monnerat Gilberto Weissmuller Luiz C Sampaio Adriana Bastos Carvalho Doris A Taylor Antonio Carlos Campos de Carvalho
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title_sort	tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix
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title_auth	Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix
abstract	Decellularized cardiac extracellular matrix scaffolds with preserved composition and architecture can be used in tissue engineering to reproduce the complex cardiac extracellular matrix. However, evaluating the extent of cardiomyocyte repopulation of decellularized cardiac extracellular matrix scaffolds after recellularization attempts is challenging. Here, we describe a unique combination of biochemical, biomechanical, histological, and physiological parameters for quantifying recellularization efficiency of tissue-engineered cardiac patches compared with native cardiac tissue. Human embryonic stem cell-derived cardiomyocytes were seeded into rat heart atrial and ventricular decellularized cardiac extracellular matrix patches. Confocal and atomic force microscopy showed cell integration within the extracellular matrix basement membrane that was accompanied by restoration of native cardiac tissue passive mechanical properties. Multi-electrode array and immunostaining (connexin 43) were used to determine synchronous field potentials with electrical coupling. Myoglobin content (~60%) and sarcomere length measurement (<45% vs 2D culture) were used to evaluate cardiomyocyte maturation of integrated cells. The combination of these techniques allowed us to demonstrate that as cellularization efficiency improves, cardiomyocytes mature and synchronize electrical activity, and tissue mechanical/biochemical properties improve toward those of native tissue.
abstractGer	Decellularized cardiac extracellular matrix scaffolds with preserved composition and architecture can be used in tissue engineering to reproduce the complex cardiac extracellular matrix. However, evaluating the extent of cardiomyocyte repopulation of decellularized cardiac extracellular matrix scaffolds after recellularization attempts is challenging. Here, we describe a unique combination of biochemical, biomechanical, histological, and physiological parameters for quantifying recellularization efficiency of tissue-engineered cardiac patches compared with native cardiac tissue. Human embryonic stem cell-derived cardiomyocytes were seeded into rat heart atrial and ventricular decellularized cardiac extracellular matrix patches. Confocal and atomic force microscopy showed cell integration within the extracellular matrix basement membrane that was accompanied by restoration of native cardiac tissue passive mechanical properties. Multi-electrode array and immunostaining (connexin 43) were used to determine synchronous field potentials with electrical coupling. Myoglobin content (~60%) and sarcomere length measurement (<45% vs 2D culture) were used to evaluate cardiomyocyte maturation of integrated cells. The combination of these techniques allowed us to demonstrate that as cellularization efficiency improves, cardiomyocytes mature and synchronize electrical activity, and tissue mechanical/biochemical properties improve toward those of native tissue.
abstract_unstemmed	Decellularized cardiac extracellular matrix scaffolds with preserved composition and architecture can be used in tissue engineering to reproduce the complex cardiac extracellular matrix. However, evaluating the extent of cardiomyocyte repopulation of decellularized cardiac extracellular matrix scaffolds after recellularization attempts is challenging. Here, we describe a unique combination of biochemical, biomechanical, histological, and physiological parameters for quantifying recellularization efficiency of tissue-engineered cardiac patches compared with native cardiac tissue. Human embryonic stem cell-derived cardiomyocytes were seeded into rat heart atrial and ventricular decellularized cardiac extracellular matrix patches. Confocal and atomic force microscopy showed cell integration within the extracellular matrix basement membrane that was accompanied by restoration of native cardiac tissue passive mechanical properties. Multi-electrode array and immunostaining (connexin 43) were used to determine synchronous field potentials with electrical coupling. Myoglobin content (~60%) and sarcomere length measurement (<45% vs 2D culture) were used to evaluate cardiomyocyte maturation of integrated cells. The combination of these techniques allowed us to demonstrate that as cellularization efficiency improves, cardiomyocytes mature and synchronize electrical activity, and tissue mechanical/biochemical properties improve toward those of native tissue.
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title_short	Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix
url	https://doi.org/10.1177/2041731420921482 https://doaj.org/article/7cfe3cd8bcee454ca01755a9a9aa1c17 https://doaj.org/toc/2041-7314
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up_date	2024-07-03T14:18:10.467Z
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Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix

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