Characterization of BoaCRTISO Reveals Its Role in Carotenoid Biosynthesis in Chinese Kale

Carotenoids are organic pigments that play an important role in both plant coloration and human health; they are a critical subject in molecular breeding due to growing demand for natural molecules in both food and medicine. In this study, we focus upon characterizing BoaCRTISO, the carotenoid isome...
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Autor*in:	Min Jiang [verfasserIn] Fen Zhang [verfasserIn] Qiao Yuan [verfasserIn] Peixing Lin [verfasserIn] Hao Zheng [verfasserIn] Sha Liang [verfasserIn] Yue Jian [verfasserIn] Huiying Miao [verfasserIn] Huanxiu Li [verfasserIn] Qiaomei Wang [verfasserIn] Bo Sun [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	BoaCRTISO carotenoid biosynthesis Chinese kale color gene expression VIGS

Übergeordnetes Werk:	In: Frontiers in Plant Science - Frontiers Media S.A., 2011, 12(2021)
Übergeordnetes Werk:	volume:12 ; year:2021

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fpls.2021.662684

Katalog-ID:	DOAJ077881060

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allfields	10.3389/fpls.2021.662684 doi (DE-627)DOAJ077881060 (DE-599)DOAJ525c90f397d442849f768eacce96a3b6 DE-627 ger DE-627 rakwb eng SB1-1110 Min Jiang verfasserin aut Characterization of BoaCRTISO Reveals Its Role in Carotenoid Biosynthesis in Chinese Kale 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Carotenoids are organic pigments that play an important role in both plant coloration and human health; they are a critical subject in molecular breeding due to growing demand for natural molecules in both food and medicine. In this study, we focus upon characterizing BoaCRTISO, the carotenoid isomerase gene before the branch of the carotenoid biosynthetic pathway, which is expressed in all organs and developmental stages of Chinese kale, and BoaCRTISO, which is located in the chloroplast. The expression of BoaCRTISO is induced by strong light, red and blue combined light, and gibberellic acid treatment, but it is suppressed by darkness and abscisic acid treatment. We obtained BoaCRTISO-silenced plants via virus-induced gene silencing technology, and the silence efficiencies ranged from 52 to 77%. The expressions of most carotenoid and chlorophyll biosynthetic genes in BoaCRTISO-silenced plants were downregulated, and the contents of carotenoids and chlorophyll were reduced. Meanwhile, BoaCRTISO-silenced plants exhibited phenotypes of yellowing leaves and inhibited growth. This functional characterization of BoaCRTISO provides insight for the biosynthesis and regulation of carotenoid in Chinese kale. BoaCRTISO carotenoid biosynthesis Chinese kale color gene expression VIGS Plant culture Fen Zhang verfasserin aut Qiao Yuan verfasserin aut Peixing Lin verfasserin aut Hao Zheng verfasserin aut Sha Liang verfasserin aut Yue Jian verfasserin aut Huiying Miao verfasserin aut Huanxiu Li verfasserin aut Qiaomei Wang verfasserin aut Bo Sun verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 12(2021) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:12 year:2021 https://doi.org/10.3389/fpls.2021.662684 kostenfrei https://doaj.org/article/525c90f397d442849f768eacce96a3b6 kostenfrei https://www.frontiersin.org/articles/10.3389/fpls.2021.662684/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021
spelling	10.3389/fpls.2021.662684 doi (DE-627)DOAJ077881060 (DE-599)DOAJ525c90f397d442849f768eacce96a3b6 DE-627 ger DE-627 rakwb eng SB1-1110 Min Jiang verfasserin aut Characterization of BoaCRTISO Reveals Its Role in Carotenoid Biosynthesis in Chinese Kale 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Carotenoids are organic pigments that play an important role in both plant coloration and human health; they are a critical subject in molecular breeding due to growing demand for natural molecules in both food and medicine. In this study, we focus upon characterizing BoaCRTISO, the carotenoid isomerase gene before the branch of the carotenoid biosynthetic pathway, which is expressed in all organs and developmental stages of Chinese kale, and BoaCRTISO, which is located in the chloroplast. The expression of BoaCRTISO is induced by strong light, red and blue combined light, and gibberellic acid treatment, but it is suppressed by darkness and abscisic acid treatment. We obtained BoaCRTISO-silenced plants via virus-induced gene silencing technology, and the silence efficiencies ranged from 52 to 77%. The expressions of most carotenoid and chlorophyll biosynthetic genes in BoaCRTISO-silenced plants were downregulated, and the contents of carotenoids and chlorophyll were reduced. Meanwhile, BoaCRTISO-silenced plants exhibited phenotypes of yellowing leaves and inhibited growth. This functional characterization of BoaCRTISO provides insight for the biosynthesis and regulation of carotenoid in Chinese kale. BoaCRTISO carotenoid biosynthesis Chinese kale color gene expression VIGS Plant culture Fen Zhang verfasserin aut Qiao Yuan verfasserin aut Peixing Lin verfasserin aut Hao Zheng verfasserin aut Sha Liang verfasserin aut Yue Jian verfasserin aut Huiying Miao verfasserin aut Huanxiu Li verfasserin aut Qiaomei Wang verfasserin aut Bo Sun verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 12(2021) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:12 year:2021 https://doi.org/10.3389/fpls.2021.662684 kostenfrei https://doaj.org/article/525c90f397d442849f768eacce96a3b6 kostenfrei https://www.frontiersin.org/articles/10.3389/fpls.2021.662684/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021
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allfieldsSound	10.3389/fpls.2021.662684 doi (DE-627)DOAJ077881060 (DE-599)DOAJ525c90f397d442849f768eacce96a3b6 DE-627 ger DE-627 rakwb eng SB1-1110 Min Jiang verfasserin aut Characterization of BoaCRTISO Reveals Its Role in Carotenoid Biosynthesis in Chinese Kale 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Carotenoids are organic pigments that play an important role in both plant coloration and human health; they are a critical subject in molecular breeding due to growing demand for natural molecules in both food and medicine. In this study, we focus upon characterizing BoaCRTISO, the carotenoid isomerase gene before the branch of the carotenoid biosynthetic pathway, which is expressed in all organs and developmental stages of Chinese kale, and BoaCRTISO, which is located in the chloroplast. The expression of BoaCRTISO is induced by strong light, red and blue combined light, and gibberellic acid treatment, but it is suppressed by darkness and abscisic acid treatment. We obtained BoaCRTISO-silenced plants via virus-induced gene silencing technology, and the silence efficiencies ranged from 52 to 77%. The expressions of most carotenoid and chlorophyll biosynthetic genes in BoaCRTISO-silenced plants were downregulated, and the contents of carotenoids and chlorophyll were reduced. Meanwhile, BoaCRTISO-silenced plants exhibited phenotypes of yellowing leaves and inhibited growth. This functional characterization of BoaCRTISO provides insight for the biosynthesis and regulation of carotenoid in Chinese kale. BoaCRTISO carotenoid biosynthesis Chinese kale color gene expression VIGS Plant culture Fen Zhang verfasserin aut Qiao Yuan verfasserin aut Peixing Lin verfasserin aut Hao Zheng verfasserin aut Sha Liang verfasserin aut Yue Jian verfasserin aut Huiying Miao verfasserin aut Huanxiu Li verfasserin aut Qiaomei Wang verfasserin aut Bo Sun verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 12(2021) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:12 year:2021 https://doi.org/10.3389/fpls.2021.662684 kostenfrei https://doaj.org/article/525c90f397d442849f768eacce96a3b6 kostenfrei https://www.frontiersin.org/articles/10.3389/fpls.2021.662684/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021
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topic_facet	BoaCRTISO carotenoid biosynthesis Chinese kale color gene expression VIGS Plant culture
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container_title	Frontiers in Plant Science
authorswithroles_txt_mv	Min Jiang @@aut@@ Fen Zhang @@aut@@ Qiao Yuan @@aut@@ Peixing Lin @@aut@@ Hao Zheng @@aut@@ Sha Liang @@aut@@ Yue Jian @@aut@@ Huiying Miao @@aut@@ Huanxiu Li @@aut@@ Qiaomei Wang @@aut@@ Bo Sun @@aut@@
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callnumber-first	S - Agriculture
author	Min Jiang
spellingShingle	Min Jiang misc SB1-1110 misc BoaCRTISO misc carotenoid biosynthesis misc Chinese kale misc color misc gene expression misc VIGS misc Plant culture Characterization of BoaCRTISO Reveals Its Role in Carotenoid Biosynthesis in Chinese Kale
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topic_title	SB1-1110 Characterization of BoaCRTISO Reveals Its Role in Carotenoid Biosynthesis in Chinese Kale BoaCRTISO carotenoid biosynthesis Chinese kale color gene expression VIGS
topic	misc SB1-1110 misc BoaCRTISO misc carotenoid biosynthesis misc Chinese kale misc color misc gene expression misc VIGS misc Plant culture
topic_unstemmed	misc SB1-1110 misc BoaCRTISO misc carotenoid biosynthesis misc Chinese kale misc color misc gene expression misc VIGS misc Plant culture
topic_browse	misc SB1-1110 misc BoaCRTISO misc carotenoid biosynthesis misc Chinese kale misc color misc gene expression misc VIGS misc Plant culture
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title	Characterization of BoaCRTISO Reveals Its Role in Carotenoid Biosynthesis in Chinese Kale
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title_full	Characterization of BoaCRTISO Reveals Its Role in Carotenoid Biosynthesis in Chinese Kale
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author_browse	Min Jiang Fen Zhang Qiao Yuan Peixing Lin Hao Zheng Sha Liang Yue Jian Huiying Miao Huanxiu Li Qiaomei Wang Bo Sun
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title_sort	characterization of boacrtiso reveals its role in carotenoid biosynthesis in chinese kale
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title_auth	Characterization of BoaCRTISO Reveals Its Role in Carotenoid Biosynthesis in Chinese Kale
abstract	Carotenoids are organic pigments that play an important role in both plant coloration and human health; they are a critical subject in molecular breeding due to growing demand for natural molecules in both food and medicine. In this study, we focus upon characterizing BoaCRTISO, the carotenoid isomerase gene before the branch of the carotenoid biosynthetic pathway, which is expressed in all organs and developmental stages of Chinese kale, and BoaCRTISO, which is located in the chloroplast. The expression of BoaCRTISO is induced by strong light, red and blue combined light, and gibberellic acid treatment, but it is suppressed by darkness and abscisic acid treatment. We obtained BoaCRTISO-silenced plants via virus-induced gene silencing technology, and the silence efficiencies ranged from 52 to 77%. The expressions of most carotenoid and chlorophyll biosynthetic genes in BoaCRTISO-silenced plants were downregulated, and the contents of carotenoids and chlorophyll were reduced. Meanwhile, BoaCRTISO-silenced plants exhibited phenotypes of yellowing leaves and inhibited growth. This functional characterization of BoaCRTISO provides insight for the biosynthesis and regulation of carotenoid in Chinese kale.
abstractGer	Carotenoids are organic pigments that play an important role in both plant coloration and human health; they are a critical subject in molecular breeding due to growing demand for natural molecules in both food and medicine. In this study, we focus upon characterizing BoaCRTISO, the carotenoid isomerase gene before the branch of the carotenoid biosynthetic pathway, which is expressed in all organs and developmental stages of Chinese kale, and BoaCRTISO, which is located in the chloroplast. The expression of BoaCRTISO is induced by strong light, red and blue combined light, and gibberellic acid treatment, but it is suppressed by darkness and abscisic acid treatment. We obtained BoaCRTISO-silenced plants via virus-induced gene silencing technology, and the silence efficiencies ranged from 52 to 77%. The expressions of most carotenoid and chlorophyll biosynthetic genes in BoaCRTISO-silenced plants were downregulated, and the contents of carotenoids and chlorophyll were reduced. Meanwhile, BoaCRTISO-silenced plants exhibited phenotypes of yellowing leaves and inhibited growth. This functional characterization of BoaCRTISO provides insight for the biosynthesis and regulation of carotenoid in Chinese kale.
abstract_unstemmed	Carotenoids are organic pigments that play an important role in both plant coloration and human health; they are a critical subject in molecular breeding due to growing demand for natural molecules in both food and medicine. In this study, we focus upon characterizing BoaCRTISO, the carotenoid isomerase gene before the branch of the carotenoid biosynthetic pathway, which is expressed in all organs and developmental stages of Chinese kale, and BoaCRTISO, which is located in the chloroplast. The expression of BoaCRTISO is induced by strong light, red and blue combined light, and gibberellic acid treatment, but it is suppressed by darkness and abscisic acid treatment. We obtained BoaCRTISO-silenced plants via virus-induced gene silencing technology, and the silence efficiencies ranged from 52 to 77%. The expressions of most carotenoid and chlorophyll biosynthetic genes in BoaCRTISO-silenced plants were downregulated, and the contents of carotenoids and chlorophyll were reduced. Meanwhile, BoaCRTISO-silenced plants exhibited phenotypes of yellowing leaves and inhibited growth. This functional characterization of BoaCRTISO provides insight for the biosynthesis and regulation of carotenoid in Chinese kale.
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title_short	Characterization of BoaCRTISO Reveals Its Role in Carotenoid Biosynthesis in Chinese Kale
url	https://doi.org/10.3389/fpls.2021.662684 https://doaj.org/article/525c90f397d442849f768eacce96a3b6 https://www.frontiersin.org/articles/10.3389/fpls.2021.662684/full https://doaj.org/toc/1664-462X
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up_date	2024-07-03T14:41:43.326Z
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Characterization of BoaCRTISO Reveals Its Role in Carotenoid Biosynthesis in Chinese Kale

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