Fourier-Transform Infrared Microspectroscopy (FT-IR) Study on Caput and Cauda Mouse Spermatozoa
Fourier-Transform Infrared micro-spectroscopy (µFT-IR) was used for an in vitro investigation on spermatozoa (SPZ) samples separately collected from caput and cauda of mouse epididymis. SPZ are characterized by deep biochemical changes during the transit along the epididymis and they can constitute...
Ausführliche Beschreibung
Autor*in: |
Marianna Portaccio [verfasserIn] Sonia Errico [verfasserIn] Teresa Chioccarelli [verfasserIn] Gilda Cobellis [verfasserIn] Maria Lepore [verfasserIn] |
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E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2020 |
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Übergeordnetes Werk: |
In: Proceedings - MDPI AG, 2018, 42(2020), 1, p 19 |
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Übergeordnetes Werk: |
volume:42 ; year:2020 ; number:1, p 19 |
Links: |
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DOI / URN: |
10.3390/ecsa-6-06537 |
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DOAJ07797414X |
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10.3390/ecsa-6-06537 doi (DE-627)DOAJ07797414X (DE-599)DOAJ08d07f6ab56e47cbbb4dd645e987df95 DE-627 ger DE-627 rakwb eng Marianna Portaccio verfasserin aut Fourier-Transform Infrared Microspectroscopy (FT-IR) Study on Caput and Cauda Mouse Spermatozoa 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Fourier-Transform Infrared micro-spectroscopy (µFT-IR) was used for an in vitro investigation on spermatozoa (SPZ) samples separately collected from caput and cauda of mouse epididymis. SPZ are characterized by deep biochemical changes during the transit along the epididymis and they can constitute ideal candidates for a µFT-IR investigation, thanks to the ability of this technique in analyzing cells at a molecular level. Appreciable differences were reported in the infrared spectra from caput and cauda SPZ, and biochemical changes in protein, nucleic acid, lipid, and carbohydrate content of cells were evidenced. The present investigation indicates that µFT-IR can constitute a valuable tool for monitoring, in an easy and fast way, the changes suffered by SPZ during the transit along the epididymis. caput and cauda spermatozoa µft-ir microspectroscopy protein nucleic acid lipid and carbohydrate components General Works A Sonia Errico verfasserin aut Teresa Chioccarelli verfasserin aut Gilda Cobellis verfasserin aut Maria Lepore verfasserin aut In Proceedings MDPI AG, 2018 42(2020), 1, p 19 (DE-627)896671828 (DE-600)2904077-2 25043900 nnns volume:42 year:2020 number:1, p 19 https://doi.org/10.3390/ecsa-6-06537 kostenfrei https://doaj.org/article/08d07f6ab56e47cbbb4dd645e987df95 kostenfrei https://www.mdpi.com/2504-3900/42/1/19 kostenfrei https://doaj.org/toc/2504-3900 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 42 2020 1, p 19 |
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10.3390/ecsa-6-06537 doi (DE-627)DOAJ07797414X (DE-599)DOAJ08d07f6ab56e47cbbb4dd645e987df95 DE-627 ger DE-627 rakwb eng Marianna Portaccio verfasserin aut Fourier-Transform Infrared Microspectroscopy (FT-IR) Study on Caput and Cauda Mouse Spermatozoa 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Fourier-Transform Infrared micro-spectroscopy (µFT-IR) was used for an in vitro investigation on spermatozoa (SPZ) samples separately collected from caput and cauda of mouse epididymis. SPZ are characterized by deep biochemical changes during the transit along the epididymis and they can constitute ideal candidates for a µFT-IR investigation, thanks to the ability of this technique in analyzing cells at a molecular level. Appreciable differences were reported in the infrared spectra from caput and cauda SPZ, and biochemical changes in protein, nucleic acid, lipid, and carbohydrate content of cells were evidenced. The present investigation indicates that µFT-IR can constitute a valuable tool for monitoring, in an easy and fast way, the changes suffered by SPZ during the transit along the epididymis. caput and cauda spermatozoa µft-ir microspectroscopy protein nucleic acid lipid and carbohydrate components General Works A Sonia Errico verfasserin aut Teresa Chioccarelli verfasserin aut Gilda Cobellis verfasserin aut Maria Lepore verfasserin aut In Proceedings MDPI AG, 2018 42(2020), 1, p 19 (DE-627)896671828 (DE-600)2904077-2 25043900 nnns volume:42 year:2020 number:1, p 19 https://doi.org/10.3390/ecsa-6-06537 kostenfrei https://doaj.org/article/08d07f6ab56e47cbbb4dd645e987df95 kostenfrei https://www.mdpi.com/2504-3900/42/1/19 kostenfrei https://doaj.org/toc/2504-3900 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 42 2020 1, p 19 |
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10.3390/ecsa-6-06537 doi (DE-627)DOAJ07797414X (DE-599)DOAJ08d07f6ab56e47cbbb4dd645e987df95 DE-627 ger DE-627 rakwb eng Marianna Portaccio verfasserin aut Fourier-Transform Infrared Microspectroscopy (FT-IR) Study on Caput and Cauda Mouse Spermatozoa 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Fourier-Transform Infrared micro-spectroscopy (µFT-IR) was used for an in vitro investigation on spermatozoa (SPZ) samples separately collected from caput and cauda of mouse epididymis. SPZ are characterized by deep biochemical changes during the transit along the epididymis and they can constitute ideal candidates for a µFT-IR investigation, thanks to the ability of this technique in analyzing cells at a molecular level. Appreciable differences were reported in the infrared spectra from caput and cauda SPZ, and biochemical changes in protein, nucleic acid, lipid, and carbohydrate content of cells were evidenced. The present investigation indicates that µFT-IR can constitute a valuable tool for monitoring, in an easy and fast way, the changes suffered by SPZ during the transit along the epididymis. caput and cauda spermatozoa µft-ir microspectroscopy protein nucleic acid lipid and carbohydrate components General Works A Sonia Errico verfasserin aut Teresa Chioccarelli verfasserin aut Gilda Cobellis verfasserin aut Maria Lepore verfasserin aut In Proceedings MDPI AG, 2018 42(2020), 1, p 19 (DE-627)896671828 (DE-600)2904077-2 25043900 nnns volume:42 year:2020 number:1, p 19 https://doi.org/10.3390/ecsa-6-06537 kostenfrei https://doaj.org/article/08d07f6ab56e47cbbb4dd645e987df95 kostenfrei https://www.mdpi.com/2504-3900/42/1/19 kostenfrei https://doaj.org/toc/2504-3900 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 42 2020 1, p 19 |
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10.3390/ecsa-6-06537 doi (DE-627)DOAJ07797414X (DE-599)DOAJ08d07f6ab56e47cbbb4dd645e987df95 DE-627 ger DE-627 rakwb eng Marianna Portaccio verfasserin aut Fourier-Transform Infrared Microspectroscopy (FT-IR) Study on Caput and Cauda Mouse Spermatozoa 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Fourier-Transform Infrared micro-spectroscopy (µFT-IR) was used for an in vitro investigation on spermatozoa (SPZ) samples separately collected from caput and cauda of mouse epididymis. SPZ are characterized by deep biochemical changes during the transit along the epididymis and they can constitute ideal candidates for a µFT-IR investigation, thanks to the ability of this technique in analyzing cells at a molecular level. Appreciable differences were reported in the infrared spectra from caput and cauda SPZ, and biochemical changes in protein, nucleic acid, lipid, and carbohydrate content of cells were evidenced. The present investigation indicates that µFT-IR can constitute a valuable tool for monitoring, in an easy and fast way, the changes suffered by SPZ during the transit along the epididymis. caput and cauda spermatozoa µft-ir microspectroscopy protein nucleic acid lipid and carbohydrate components General Works A Sonia Errico verfasserin aut Teresa Chioccarelli verfasserin aut Gilda Cobellis verfasserin aut Maria Lepore verfasserin aut In Proceedings MDPI AG, 2018 42(2020), 1, p 19 (DE-627)896671828 (DE-600)2904077-2 25043900 nnns volume:42 year:2020 number:1, p 19 https://doi.org/10.3390/ecsa-6-06537 kostenfrei https://doaj.org/article/08d07f6ab56e47cbbb4dd645e987df95 kostenfrei https://www.mdpi.com/2504-3900/42/1/19 kostenfrei https://doaj.org/toc/2504-3900 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 42 2020 1, p 19 |
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Fourier-Transform Infrared Microspectroscopy (FT-IR) Study on Caput and Cauda Mouse Spermatozoa caput and cauda spermatozoa µft-ir microspectroscopy protein nucleic acid lipid and carbohydrate components |
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Fourier-Transform Infrared Microspectroscopy (FT-IR) Study on Caput and Cauda Mouse Spermatozoa |
abstract |
Fourier-Transform Infrared micro-spectroscopy (µFT-IR) was used for an in vitro investigation on spermatozoa (SPZ) samples separately collected from caput and cauda of mouse epididymis. SPZ are characterized by deep biochemical changes during the transit along the epididymis and they can constitute ideal candidates for a µFT-IR investigation, thanks to the ability of this technique in analyzing cells at a molecular level. Appreciable differences were reported in the infrared spectra from caput and cauda SPZ, and biochemical changes in protein, nucleic acid, lipid, and carbohydrate content of cells were evidenced. The present investigation indicates that µFT-IR can constitute a valuable tool for monitoring, in an easy and fast way, the changes suffered by SPZ during the transit along the epididymis. |
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Fourier-Transform Infrared micro-spectroscopy (µFT-IR) was used for an in vitro investigation on spermatozoa (SPZ) samples separately collected from caput and cauda of mouse epididymis. SPZ are characterized by deep biochemical changes during the transit along the epididymis and they can constitute ideal candidates for a µFT-IR investigation, thanks to the ability of this technique in analyzing cells at a molecular level. Appreciable differences were reported in the infrared spectra from caput and cauda SPZ, and biochemical changes in protein, nucleic acid, lipid, and carbohydrate content of cells were evidenced. The present investigation indicates that µFT-IR can constitute a valuable tool for monitoring, in an easy and fast way, the changes suffered by SPZ during the transit along the epididymis. |
abstract_unstemmed |
Fourier-Transform Infrared micro-spectroscopy (µFT-IR) was used for an in vitro investigation on spermatozoa (SPZ) samples separately collected from caput and cauda of mouse epididymis. SPZ are characterized by deep biochemical changes during the transit along the epididymis and they can constitute ideal candidates for a µFT-IR investigation, thanks to the ability of this technique in analyzing cells at a molecular level. Appreciable differences were reported in the infrared spectra from caput and cauda SPZ, and biochemical changes in protein, nucleic acid, lipid, and carbohydrate content of cells were evidenced. The present investigation indicates that µFT-IR can constitute a valuable tool for monitoring, in an easy and fast way, the changes suffered by SPZ during the transit along the epididymis. |
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|
score |
7.4032135 |