In vitro evaluation of P-glycoprotein functions in human neuroblastoma cell lines
Background: Neuroblastoma (NB) remains one of the most puzzling of paediatric cancers in which most patients develop progressive disease that is refractory to chemotherapy. Effective treatment is hampered by drug resistance related to the expression of multidrug-resistant proteins belonging to the A...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Krai Daowtak [verfasserIn] Suchart Kothan [verfasserIn] Suhaida Doloh [verfasserIn] Wiyada Dankai [verfasserIn] Chatchanok Udomtanakunchai [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2018 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
In: Journal of Associated Medical Sciences - Chaing Mai University, 2019, 51(2018), 2, Seite 72-80 |
|---|---|
| Übergeordnetes Werk: |
volume:51 ; year:2018 ; number:2 ; pages:72-80 |
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DOAJ078078008 |
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| 520 | |a Background: Neuroblastoma (NB) remains one of the most puzzling of paediatric cancers in which most patients develop progressive disease that is refractory to chemotherapy. Effective treatment is hampered by drug resistance related to the expression of multidrug-resistant proteins belonging to the ATP-binding cassette transporters family, especially P-glycoprotein (P-gp). Most previous studies focused on molecular evidence of P-gp expression, however, functional studies of P-gp efflux have not clearly demonstrated. Objectives: The aim of this study was to determine whether human neuroblastoma cell lines (SH-SY5Y and SK-N-SH) express the functionally active P-gp efflux pump. Materials and methods: Functional studies on P-gp–mediated pumping were performed using pirarubicin, a P-gp substrate, with verapamil, a multidrug resistance inhibitor, and analyzed by a spectrofluorometer. To confirm the gene expression, reverse transcription polymerase chain reaction (RT-PCR) was performed with specific primers for human multidrug resistance 1 (MDR1). Results: MDR1 expression was observed in neuroblastoma cell line (SH-SY5Y) in the same degree of expression as in the sensitive K562 cell line, a negative P-gp model. Kinetic analysis showed that there was no difference in drug accumulation in the presence or absence of verapamil, indicating that no function of P-gp influenced the accumulation of pirarubicin (PIRA) in both human neuroblastoma cell lines. Combination treatment of verapamil and PIRA was also not found to increase the sensitivity of PIRA. Conclusion: This study suggests that the existence of P-gp in neuroblastoma cell lines is not significant function. | ||
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(DE-627)DOAJ078078008 (DE-599)DOAJc1b67051245f44abb4ab39b800063003 DE-627 ger DE-627 rakwb eng Krai Daowtak verfasserin aut In vitro evaluation of P-glycoprotein functions in human neuroblastoma cell lines 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Neuroblastoma (NB) remains one of the most puzzling of paediatric cancers in which most patients develop progressive disease that is refractory to chemotherapy. Effective treatment is hampered by drug resistance related to the expression of multidrug-resistant proteins belonging to the ATP-binding cassette transporters family, especially P-glycoprotein (P-gp). Most previous studies focused on molecular evidence of P-gp expression, however, functional studies of P-gp efflux have not clearly demonstrated. Objectives: The aim of this study was to determine whether human neuroblastoma cell lines (SH-SY5Y and SK-N-SH) express the functionally active P-gp efflux pump. Materials and methods: Functional studies on P-gp–mediated pumping were performed using pirarubicin, a P-gp substrate, with verapamil, a multidrug resistance inhibitor, and analyzed by a spectrofluorometer. To confirm the gene expression, reverse transcription polymerase chain reaction (RT-PCR) was performed with specific primers for human multidrug resistance 1 (MDR1). Results: MDR1 expression was observed in neuroblastoma cell line (SH-SY5Y) in the same degree of expression as in the sensitive K562 cell line, a negative P-gp model. Kinetic analysis showed that there was no difference in drug accumulation in the presence or absence of verapamil, indicating that no function of P-gp influenced the accumulation of pirarubicin (PIRA) in both human neuroblastoma cell lines. Combination treatment of verapamil and PIRA was also not found to increase the sensitivity of PIRA. Conclusion: This study suggests that the existence of P-gp in neuroblastoma cell lines is not significant function. Chemotherapy drug resistance neuroblastoma P-glycoprotein Medicine R Suchart Kothan verfasserin aut Suhaida Doloh verfasserin aut Wiyada Dankai verfasserin aut Chatchanok Udomtanakunchai verfasserin aut In Journal of Associated Medical Sciences Chaing Mai University, 2019 51(2018), 2, Seite 72-80 (DE-627)1760637769 25396056 nnns volume:51 year:2018 number:2 pages:72-80 https://doaj.org/article/c1b67051245f44abb4ab39b800063003 kostenfrei https://www.tci-thaijo.org/index.php/bulletinAMS/article/view/104482 kostenfrei https://doaj.org/toc/2539-6056 Journal toc kostenfrei https://doaj.org/toc/2539-6056 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 51 2018 2 72-80 |
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(DE-627)DOAJ078078008 (DE-599)DOAJc1b67051245f44abb4ab39b800063003 DE-627 ger DE-627 rakwb eng Krai Daowtak verfasserin aut In vitro evaluation of P-glycoprotein functions in human neuroblastoma cell lines 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Neuroblastoma (NB) remains one of the most puzzling of paediatric cancers in which most patients develop progressive disease that is refractory to chemotherapy. Effective treatment is hampered by drug resistance related to the expression of multidrug-resistant proteins belonging to the ATP-binding cassette transporters family, especially P-glycoprotein (P-gp). Most previous studies focused on molecular evidence of P-gp expression, however, functional studies of P-gp efflux have not clearly demonstrated. Objectives: The aim of this study was to determine whether human neuroblastoma cell lines (SH-SY5Y and SK-N-SH) express the functionally active P-gp efflux pump. Materials and methods: Functional studies on P-gp–mediated pumping were performed using pirarubicin, a P-gp substrate, with verapamil, a multidrug resistance inhibitor, and analyzed by a spectrofluorometer. To confirm the gene expression, reverse transcription polymerase chain reaction (RT-PCR) was performed with specific primers for human multidrug resistance 1 (MDR1). Results: MDR1 expression was observed in neuroblastoma cell line (SH-SY5Y) in the same degree of expression as in the sensitive K562 cell line, a negative P-gp model. Kinetic analysis showed that there was no difference in drug accumulation in the presence or absence of verapamil, indicating that no function of P-gp influenced the accumulation of pirarubicin (PIRA) in both human neuroblastoma cell lines. Combination treatment of verapamil and PIRA was also not found to increase the sensitivity of PIRA. Conclusion: This study suggests that the existence of P-gp in neuroblastoma cell lines is not significant function. Chemotherapy drug resistance neuroblastoma P-glycoprotein Medicine R Suchart Kothan verfasserin aut Suhaida Doloh verfasserin aut Wiyada Dankai verfasserin aut Chatchanok Udomtanakunchai verfasserin aut In Journal of Associated Medical Sciences Chaing Mai University, 2019 51(2018), 2, Seite 72-80 (DE-627)1760637769 25396056 nnns volume:51 year:2018 number:2 pages:72-80 https://doaj.org/article/c1b67051245f44abb4ab39b800063003 kostenfrei https://www.tci-thaijo.org/index.php/bulletinAMS/article/view/104482 kostenfrei https://doaj.org/toc/2539-6056 Journal toc kostenfrei https://doaj.org/toc/2539-6056 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 51 2018 2 72-80 |
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(DE-627)DOAJ078078008 (DE-599)DOAJc1b67051245f44abb4ab39b800063003 DE-627 ger DE-627 rakwb eng Krai Daowtak verfasserin aut In vitro evaluation of P-glycoprotein functions in human neuroblastoma cell lines 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Neuroblastoma (NB) remains one of the most puzzling of paediatric cancers in which most patients develop progressive disease that is refractory to chemotherapy. Effective treatment is hampered by drug resistance related to the expression of multidrug-resistant proteins belonging to the ATP-binding cassette transporters family, especially P-glycoprotein (P-gp). Most previous studies focused on molecular evidence of P-gp expression, however, functional studies of P-gp efflux have not clearly demonstrated. Objectives: The aim of this study was to determine whether human neuroblastoma cell lines (SH-SY5Y and SK-N-SH) express the functionally active P-gp efflux pump. Materials and methods: Functional studies on P-gp–mediated pumping were performed using pirarubicin, a P-gp substrate, with verapamil, a multidrug resistance inhibitor, and analyzed by a spectrofluorometer. To confirm the gene expression, reverse transcription polymerase chain reaction (RT-PCR) was performed with specific primers for human multidrug resistance 1 (MDR1). Results: MDR1 expression was observed in neuroblastoma cell line (SH-SY5Y) in the same degree of expression as in the sensitive K562 cell line, a negative P-gp model. Kinetic analysis showed that there was no difference in drug accumulation in the presence or absence of verapamil, indicating that no function of P-gp influenced the accumulation of pirarubicin (PIRA) in both human neuroblastoma cell lines. Combination treatment of verapamil and PIRA was also not found to increase the sensitivity of PIRA. Conclusion: This study suggests that the existence of P-gp in neuroblastoma cell lines is not significant function. Chemotherapy drug resistance neuroblastoma P-glycoprotein Medicine R Suchart Kothan verfasserin aut Suhaida Doloh verfasserin aut Wiyada Dankai verfasserin aut Chatchanok Udomtanakunchai verfasserin aut In Journal of Associated Medical Sciences Chaing Mai University, 2019 51(2018), 2, Seite 72-80 (DE-627)1760637769 25396056 nnns volume:51 year:2018 number:2 pages:72-80 https://doaj.org/article/c1b67051245f44abb4ab39b800063003 kostenfrei https://www.tci-thaijo.org/index.php/bulletinAMS/article/view/104482 kostenfrei https://doaj.org/toc/2539-6056 Journal toc kostenfrei https://doaj.org/toc/2539-6056 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 51 2018 2 72-80 |
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(DE-627)DOAJ078078008 (DE-599)DOAJc1b67051245f44abb4ab39b800063003 DE-627 ger DE-627 rakwb eng Krai Daowtak verfasserin aut In vitro evaluation of P-glycoprotein functions in human neuroblastoma cell lines 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Neuroblastoma (NB) remains one of the most puzzling of paediatric cancers in which most patients develop progressive disease that is refractory to chemotherapy. Effective treatment is hampered by drug resistance related to the expression of multidrug-resistant proteins belonging to the ATP-binding cassette transporters family, especially P-glycoprotein (P-gp). Most previous studies focused on molecular evidence of P-gp expression, however, functional studies of P-gp efflux have not clearly demonstrated. Objectives: The aim of this study was to determine whether human neuroblastoma cell lines (SH-SY5Y and SK-N-SH) express the functionally active P-gp efflux pump. Materials and methods: Functional studies on P-gp–mediated pumping were performed using pirarubicin, a P-gp substrate, with verapamil, a multidrug resistance inhibitor, and analyzed by a spectrofluorometer. To confirm the gene expression, reverse transcription polymerase chain reaction (RT-PCR) was performed with specific primers for human multidrug resistance 1 (MDR1). Results: MDR1 expression was observed in neuroblastoma cell line (SH-SY5Y) in the same degree of expression as in the sensitive K562 cell line, a negative P-gp model. Kinetic analysis showed that there was no difference in drug accumulation in the presence or absence of verapamil, indicating that no function of P-gp influenced the accumulation of pirarubicin (PIRA) in both human neuroblastoma cell lines. Combination treatment of verapamil and PIRA was also not found to increase the sensitivity of PIRA. Conclusion: This study suggests that the existence of P-gp in neuroblastoma cell lines is not significant function. Chemotherapy drug resistance neuroblastoma P-glycoprotein Medicine R Suchart Kothan verfasserin aut Suhaida Doloh verfasserin aut Wiyada Dankai verfasserin aut Chatchanok Udomtanakunchai verfasserin aut In Journal of Associated Medical Sciences Chaing Mai University, 2019 51(2018), 2, Seite 72-80 (DE-627)1760637769 25396056 nnns volume:51 year:2018 number:2 pages:72-80 https://doaj.org/article/c1b67051245f44abb4ab39b800063003 kostenfrei https://www.tci-thaijo.org/index.php/bulletinAMS/article/view/104482 kostenfrei https://doaj.org/toc/2539-6056 Journal toc kostenfrei https://doaj.org/toc/2539-6056 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 51 2018 2 72-80 |
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(DE-627)DOAJ078078008 (DE-599)DOAJc1b67051245f44abb4ab39b800063003 DE-627 ger DE-627 rakwb eng Krai Daowtak verfasserin aut In vitro evaluation of P-glycoprotein functions in human neuroblastoma cell lines 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Neuroblastoma (NB) remains one of the most puzzling of paediatric cancers in which most patients develop progressive disease that is refractory to chemotherapy. Effective treatment is hampered by drug resistance related to the expression of multidrug-resistant proteins belonging to the ATP-binding cassette transporters family, especially P-glycoprotein (P-gp). Most previous studies focused on molecular evidence of P-gp expression, however, functional studies of P-gp efflux have not clearly demonstrated. Objectives: The aim of this study was to determine whether human neuroblastoma cell lines (SH-SY5Y and SK-N-SH) express the functionally active P-gp efflux pump. Materials and methods: Functional studies on P-gp–mediated pumping were performed using pirarubicin, a P-gp substrate, with verapamil, a multidrug resistance inhibitor, and analyzed by a spectrofluorometer. To confirm the gene expression, reverse transcription polymerase chain reaction (RT-PCR) was performed with specific primers for human multidrug resistance 1 (MDR1). Results: MDR1 expression was observed in neuroblastoma cell line (SH-SY5Y) in the same degree of expression as in the sensitive K562 cell line, a negative P-gp model. Kinetic analysis showed that there was no difference in drug accumulation in the presence or absence of verapamil, indicating that no function of P-gp influenced the accumulation of pirarubicin (PIRA) in both human neuroblastoma cell lines. Combination treatment of verapamil and PIRA was also not found to increase the sensitivity of PIRA. Conclusion: This study suggests that the existence of P-gp in neuroblastoma cell lines is not significant function. Chemotherapy drug resistance neuroblastoma P-glycoprotein Medicine R Suchart Kothan verfasserin aut Suhaida Doloh verfasserin aut Wiyada Dankai verfasserin aut Chatchanok Udomtanakunchai verfasserin aut In Journal of Associated Medical Sciences Chaing Mai University, 2019 51(2018), 2, Seite 72-80 (DE-627)1760637769 25396056 nnns volume:51 year:2018 number:2 pages:72-80 https://doaj.org/article/c1b67051245f44abb4ab39b800063003 kostenfrei https://www.tci-thaijo.org/index.php/bulletinAMS/article/view/104482 kostenfrei https://doaj.org/toc/2539-6056 Journal toc kostenfrei https://doaj.org/toc/2539-6056 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 51 2018 2 72-80 |
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In vitro evaluation of P-glycoprotein functions in human neuroblastoma cell lines |
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Krai Daowtak |
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Journal of Associated Medical Sciences |
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Journal of Associated Medical Sciences |
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Krai Daowtak Suchart Kothan Suhaida Doloh Wiyada Dankai Chatchanok Udomtanakunchai |
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Krai Daowtak |
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verfasserin |
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in vitro evaluation of p-glycoprotein functions in human neuroblastoma cell lines |
| title_auth |
In vitro evaluation of P-glycoprotein functions in human neuroblastoma cell lines |
| abstract |
Background: Neuroblastoma (NB) remains one of the most puzzling of paediatric cancers in which most patients develop progressive disease that is refractory to chemotherapy. Effective treatment is hampered by drug resistance related to the expression of multidrug-resistant proteins belonging to the ATP-binding cassette transporters family, especially P-glycoprotein (P-gp). Most previous studies focused on molecular evidence of P-gp expression, however, functional studies of P-gp efflux have not clearly demonstrated. Objectives: The aim of this study was to determine whether human neuroblastoma cell lines (SH-SY5Y and SK-N-SH) express the functionally active P-gp efflux pump. Materials and methods: Functional studies on P-gp–mediated pumping were performed using pirarubicin, a P-gp substrate, with verapamil, a multidrug resistance inhibitor, and analyzed by a spectrofluorometer. To confirm the gene expression, reverse transcription polymerase chain reaction (RT-PCR) was performed with specific primers for human multidrug resistance 1 (MDR1). Results: MDR1 expression was observed in neuroblastoma cell line (SH-SY5Y) in the same degree of expression as in the sensitive K562 cell line, a negative P-gp model. Kinetic analysis showed that there was no difference in drug accumulation in the presence or absence of verapamil, indicating that no function of P-gp influenced the accumulation of pirarubicin (PIRA) in both human neuroblastoma cell lines. Combination treatment of verapamil and PIRA was also not found to increase the sensitivity of PIRA. Conclusion: This study suggests that the existence of P-gp in neuroblastoma cell lines is not significant function. |
| abstractGer |
Background: Neuroblastoma (NB) remains one of the most puzzling of paediatric cancers in which most patients develop progressive disease that is refractory to chemotherapy. Effective treatment is hampered by drug resistance related to the expression of multidrug-resistant proteins belonging to the ATP-binding cassette transporters family, especially P-glycoprotein (P-gp). Most previous studies focused on molecular evidence of P-gp expression, however, functional studies of P-gp efflux have not clearly demonstrated. Objectives: The aim of this study was to determine whether human neuroblastoma cell lines (SH-SY5Y and SK-N-SH) express the functionally active P-gp efflux pump. Materials and methods: Functional studies on P-gp–mediated pumping were performed using pirarubicin, a P-gp substrate, with verapamil, a multidrug resistance inhibitor, and analyzed by a spectrofluorometer. To confirm the gene expression, reverse transcription polymerase chain reaction (RT-PCR) was performed with specific primers for human multidrug resistance 1 (MDR1). Results: MDR1 expression was observed in neuroblastoma cell line (SH-SY5Y) in the same degree of expression as in the sensitive K562 cell line, a negative P-gp model. Kinetic analysis showed that there was no difference in drug accumulation in the presence or absence of verapamil, indicating that no function of P-gp influenced the accumulation of pirarubicin (PIRA) in both human neuroblastoma cell lines. Combination treatment of verapamil and PIRA was also not found to increase the sensitivity of PIRA. Conclusion: This study suggests that the existence of P-gp in neuroblastoma cell lines is not significant function. |
| abstract_unstemmed |
Background: Neuroblastoma (NB) remains one of the most puzzling of paediatric cancers in which most patients develop progressive disease that is refractory to chemotherapy. Effective treatment is hampered by drug resistance related to the expression of multidrug-resistant proteins belonging to the ATP-binding cassette transporters family, especially P-glycoprotein (P-gp). Most previous studies focused on molecular evidence of P-gp expression, however, functional studies of P-gp efflux have not clearly demonstrated. Objectives: The aim of this study was to determine whether human neuroblastoma cell lines (SH-SY5Y and SK-N-SH) express the functionally active P-gp efflux pump. Materials and methods: Functional studies on P-gp–mediated pumping were performed using pirarubicin, a P-gp substrate, with verapamil, a multidrug resistance inhibitor, and analyzed by a spectrofluorometer. To confirm the gene expression, reverse transcription polymerase chain reaction (RT-PCR) was performed with specific primers for human multidrug resistance 1 (MDR1). Results: MDR1 expression was observed in neuroblastoma cell line (SH-SY5Y) in the same degree of expression as in the sensitive K562 cell line, a negative P-gp model. Kinetic analysis showed that there was no difference in drug accumulation in the presence or absence of verapamil, indicating that no function of P-gp influenced the accumulation of pirarubicin (PIRA) in both human neuroblastoma cell lines. Combination treatment of verapamil and PIRA was also not found to increase the sensitivity of PIRA. Conclusion: This study suggests that the existence of P-gp in neuroblastoma cell lines is not significant function. |
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In vitro evaluation of P-glycoprotein functions in human neuroblastoma cell lines |
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https://doaj.org/article/c1b67051245f44abb4ab39b800063003 https://www.tci-thaijo.org/index.php/bulletinAMS/article/view/104482 https://doaj.org/toc/2539-6056 |
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Suchart Kothan Suhaida Doloh Wiyada Dankai Chatchanok Udomtanakunchai |
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2024-07-03T15:49:30.544Z |
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