Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor

A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albuminuria in urine samples. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration o...
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Autor*in:	Wireeya Chawjiraphan [verfasserIn] Chayachon Apiwat [verfasserIn] Khoonsake Segkhoonthod [verfasserIn] Kiatnida Treerattrakoon [verfasserIn] Preedee Pinpradup [verfasserIn] Nuankanya Sathirapongsasuti [verfasserIn] Prapasiri Pongprayoon [verfasserIn] Patraporn Luksirikul [verfasserIn] Patcharee Isarankura-Na-Ayudhya [verfasserIn] Deanpen Japrung [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	Albuminuria Human serum albumin Chronic kidney disease Aptasensor Graphene oxide Fluorescence quenching

Übergeordnetes Werk:	In: MethodsX - Elsevier, 2015, 7(2020), Seite 101114-
Übergeordnetes Werk:	volume:7 ; year:2020 ; pages:101114-

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.mex.2020.101114

Katalog-ID:	DOAJ078412153

Internformat


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520			\|a A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albuminuria in urine samples. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration of human serum albumin in urine. The limit of detection of the developed platform is 0.05 µg.mL−1 and the detection range is 0.1–600 µg.mL−1, which covers the albuminuria concentration range present in normal human urine and the urine of the patient with chronic kidney disease. This approach can be modified to measure albuminuria using a high-throughput quantification platform and portable point of care testing. In addition, the production cost for one reaction is cheaper than those for the standard automated method. Therefore, this aptasensor has significant potential for commercialization and public use. • Our protocol is customized by using the fluorescence quenching property of graphene oxide and specific binding property of human serum albumin aptamer to detect human serum albumin in urine sample • The limit of detection of our developed platform is 0.05 µg.mL−1 • The detection range of our aptasensor is 0.1–600 µg.mL−1
650		4	\|a Albuminuria
650		4	\|a Human serum albumin
650		4	\|a Chronic kidney disease
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650		4	\|a Fluorescence quenching
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publishDate	2020
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spelling	10.1016/j.mex.2020.101114 doi (DE-627)DOAJ078412153 (DE-599)DOAJd604f2bf1f5b4a26baeeec4d18b6858f DE-627 ger DE-627 rakwb eng Wireeya Chawjiraphan verfasserin aut Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albuminuria in urine samples. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration of human serum albumin in urine. The limit of detection of the developed platform is 0.05 µg.mL−1 and the detection range is 0.1–600 µg.mL−1, which covers the albuminuria concentration range present in normal human urine and the urine of the patient with chronic kidney disease. This approach can be modified to measure albuminuria using a high-throughput quantification platform and portable point of care testing. In addition, the production cost for one reaction is cheaper than those for the standard automated method. Therefore, this aptasensor has significant potential for commercialization and public use. • Our protocol is customized by using the fluorescence quenching property of graphene oxide and specific binding property of human serum albumin aptamer to detect human serum albumin in urine sample • The limit of detection of our developed platform is 0.05 µg.mL−1 • The detection range of our aptasensor is 0.1–600 µg.mL−1 Albuminuria Human serum albumin Chronic kidney disease Aptasensor Graphene oxide Fluorescence quenching Science Q Chayachon Apiwat verfasserin aut Khoonsake Segkhoonthod verfasserin aut Kiatnida Treerattrakoon verfasserin aut Preedee Pinpradup verfasserin aut Nuankanya Sathirapongsasuti verfasserin aut Prapasiri Pongprayoon verfasserin aut Patraporn Luksirikul verfasserin aut Patcharee Isarankura-Na-Ayudhya verfasserin aut Deanpen Japrung verfasserin aut In MethodsX Elsevier, 2015 7(2020), Seite 101114- (DE-627)832786675 (DE-600)2830212-6 22150161 nnns volume:7 year:2020 pages:101114- https://doi.org/10.1016/j.mex.2020.101114 kostenfrei https://doaj.org/article/d604f2bf1f5b4a26baeeec4d18b6858f kostenfrei http://www.sciencedirect.com/science/article/pii/S2215016120303344 kostenfrei https://doaj.org/toc/2215-0161 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 7 2020 101114-
allfields_unstemmed	10.1016/j.mex.2020.101114 doi (DE-627)DOAJ078412153 (DE-599)DOAJd604f2bf1f5b4a26baeeec4d18b6858f DE-627 ger DE-627 rakwb eng Wireeya Chawjiraphan verfasserin aut Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albuminuria in urine samples. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration of human serum albumin in urine. The limit of detection of the developed platform is 0.05 µg.mL−1 and the detection range is 0.1–600 µg.mL−1, which covers the albuminuria concentration range present in normal human urine and the urine of the patient with chronic kidney disease. This approach can be modified to measure albuminuria using a high-throughput quantification platform and portable point of care testing. In addition, the production cost for one reaction is cheaper than those for the standard automated method. Therefore, this aptasensor has significant potential for commercialization and public use. • Our protocol is customized by using the fluorescence quenching property of graphene oxide and specific binding property of human serum albumin aptamer to detect human serum albumin in urine sample • The limit of detection of our developed platform is 0.05 µg.mL−1 • The detection range of our aptasensor is 0.1–600 µg.mL−1 Albuminuria Human serum albumin Chronic kidney disease Aptasensor Graphene oxide Fluorescence quenching Science Q Chayachon Apiwat verfasserin aut Khoonsake Segkhoonthod verfasserin aut Kiatnida Treerattrakoon verfasserin aut Preedee Pinpradup verfasserin aut Nuankanya Sathirapongsasuti verfasserin aut Prapasiri Pongprayoon verfasserin aut Patraporn Luksirikul verfasserin aut Patcharee Isarankura-Na-Ayudhya verfasserin aut Deanpen Japrung verfasserin aut In MethodsX Elsevier, 2015 7(2020), Seite 101114- (DE-627)832786675 (DE-600)2830212-6 22150161 nnns volume:7 year:2020 pages:101114- https://doi.org/10.1016/j.mex.2020.101114 kostenfrei https://doaj.org/article/d604f2bf1f5b4a26baeeec4d18b6858f kostenfrei http://www.sciencedirect.com/science/article/pii/S2215016120303344 kostenfrei https://doaj.org/toc/2215-0161 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 7 2020 101114-
allfieldsGer	10.1016/j.mex.2020.101114 doi (DE-627)DOAJ078412153 (DE-599)DOAJd604f2bf1f5b4a26baeeec4d18b6858f DE-627 ger DE-627 rakwb eng Wireeya Chawjiraphan verfasserin aut Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albuminuria in urine samples. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration of human serum albumin in urine. The limit of detection of the developed platform is 0.05 µg.mL−1 and the detection range is 0.1–600 µg.mL−1, which covers the albuminuria concentration range present in normal human urine and the urine of the patient with chronic kidney disease. This approach can be modified to measure albuminuria using a high-throughput quantification platform and portable point of care testing. In addition, the production cost for one reaction is cheaper than those for the standard automated method. Therefore, this aptasensor has significant potential for commercialization and public use. • Our protocol is customized by using the fluorescence quenching property of graphene oxide and specific binding property of human serum albumin aptamer to detect human serum albumin in urine sample • The limit of detection of our developed platform is 0.05 µg.mL−1 • The detection range of our aptasensor is 0.1–600 µg.mL−1 Albuminuria Human serum albumin Chronic kidney disease Aptasensor Graphene oxide Fluorescence quenching Science Q Chayachon Apiwat verfasserin aut Khoonsake Segkhoonthod verfasserin aut Kiatnida Treerattrakoon verfasserin aut Preedee Pinpradup verfasserin aut Nuankanya Sathirapongsasuti verfasserin aut Prapasiri Pongprayoon verfasserin aut Patraporn Luksirikul verfasserin aut Patcharee Isarankura-Na-Ayudhya verfasserin aut Deanpen Japrung verfasserin aut In MethodsX Elsevier, 2015 7(2020), Seite 101114- (DE-627)832786675 (DE-600)2830212-6 22150161 nnns volume:7 year:2020 pages:101114- https://doi.org/10.1016/j.mex.2020.101114 kostenfrei https://doaj.org/article/d604f2bf1f5b4a26baeeec4d18b6858f kostenfrei http://www.sciencedirect.com/science/article/pii/S2215016120303344 kostenfrei https://doaj.org/toc/2215-0161 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 7 2020 101114-
allfieldsSound	10.1016/j.mex.2020.101114 doi (DE-627)DOAJ078412153 (DE-599)DOAJd604f2bf1f5b4a26baeeec4d18b6858f DE-627 ger DE-627 rakwb eng Wireeya Chawjiraphan verfasserin aut Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albuminuria in urine samples. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration of human serum albumin in urine. The limit of detection of the developed platform is 0.05 µg.mL−1 and the detection range is 0.1–600 µg.mL−1, which covers the albuminuria concentration range present in normal human urine and the urine of the patient with chronic kidney disease. This approach can be modified to measure albuminuria using a high-throughput quantification platform and portable point of care testing. In addition, the production cost for one reaction is cheaper than those for the standard automated method. Therefore, this aptasensor has significant potential for commercialization and public use. • Our protocol is customized by using the fluorescence quenching property of graphene oxide and specific binding property of human serum albumin aptamer to detect human serum albumin in urine sample • The limit of detection of our developed platform is 0.05 µg.mL−1 • The detection range of our aptasensor is 0.1–600 µg.mL−1 Albuminuria Human serum albumin Chronic kidney disease Aptasensor Graphene oxide Fluorescence quenching Science Q Chayachon Apiwat verfasserin aut Khoonsake Segkhoonthod verfasserin aut Kiatnida Treerattrakoon verfasserin aut Preedee Pinpradup verfasserin aut Nuankanya Sathirapongsasuti verfasserin aut Prapasiri Pongprayoon verfasserin aut Patraporn Luksirikul verfasserin aut Patcharee Isarankura-Na-Ayudhya verfasserin aut Deanpen Japrung verfasserin aut In MethodsX Elsevier, 2015 7(2020), Seite 101114- (DE-627)832786675 (DE-600)2830212-6 22150161 nnns volume:7 year:2020 pages:101114- https://doi.org/10.1016/j.mex.2020.101114 kostenfrei https://doaj.org/article/d604f2bf1f5b4a26baeeec4d18b6858f kostenfrei http://www.sciencedirect.com/science/article/pii/S2215016120303344 kostenfrei https://doaj.org/toc/2215-0161 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 7 2020 101114-
language	English
source	In MethodsX 7(2020), Seite 101114- volume:7 year:2020 pages:101114-
sourceStr	In MethodsX 7(2020), Seite 101114- volume:7 year:2020 pages:101114-
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Albuminuria Human serum albumin Chronic kidney disease Aptasensor Graphene oxide Fluorescence quenching Science Q
isfreeaccess_bool	true
container_title	MethodsX
authorswithroles_txt_mv	Wireeya Chawjiraphan @@aut@@ Chayachon Apiwat @@aut@@ Khoonsake Segkhoonthod @@aut@@ Kiatnida Treerattrakoon @@aut@@ Preedee Pinpradup @@aut@@ Nuankanya Sathirapongsasuti @@aut@@ Prapasiri Pongprayoon @@aut@@ Patraporn Luksirikul @@aut@@ Patcharee Isarankura-Na-Ayudhya @@aut@@ Deanpen Japrung @@aut@@
publishDateDaySort_date	2020-01-01T00:00:00Z
hierarchy_top_id	832786675
id	DOAJ078412153
language_de	englisch
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author	Wireeya Chawjiraphan
spellingShingle	Wireeya Chawjiraphan misc Albuminuria misc Human serum albumin misc Chronic kidney disease misc Aptasensor misc Graphene oxide misc Fluorescence quenching misc Science misc Q Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor
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topic_title	Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor Albuminuria Human serum albumin Chronic kidney disease Aptasensor Graphene oxide Fluorescence quenching
topic	misc Albuminuria misc Human serum albumin misc Chronic kidney disease misc Aptasensor misc Graphene oxide misc Fluorescence quenching misc Science misc Q
topic_unstemmed	misc Albuminuria misc Human serum albumin misc Chronic kidney disease misc Aptasensor misc Graphene oxide misc Fluorescence quenching misc Science misc Q
topic_browse	misc Albuminuria misc Human serum albumin misc Chronic kidney disease misc Aptasensor misc Graphene oxide misc Fluorescence quenching misc Science misc Q
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title	Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor
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title_full	Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor
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author_browse	Wireeya Chawjiraphan Chayachon Apiwat Khoonsake Segkhoonthod Kiatnida Treerattrakoon Preedee Pinpradup Nuankanya Sathirapongsasuti Prapasiri Pongprayoon Patraporn Luksirikul Patcharee Isarankura-Na-Ayudhya Deanpen Japrung
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title_sort	albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor
title_auth	Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor
abstract	A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albuminuria in urine samples. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration of human serum albumin in urine. The limit of detection of the developed platform is 0.05 µg.mL−1 and the detection range is 0.1–600 µg.mL−1, which covers the albuminuria concentration range present in normal human urine and the urine of the patient with chronic kidney disease. This approach can be modified to measure albuminuria using a high-throughput quantification platform and portable point of care testing. In addition, the production cost for one reaction is cheaper than those for the standard automated method. Therefore, this aptasensor has significant potential for commercialization and public use. • Our protocol is customized by using the fluorescence quenching property of graphene oxide and specific binding property of human serum albumin aptamer to detect human serum albumin in urine sample • The limit of detection of our developed platform is 0.05 µg.mL−1 • The detection range of our aptasensor is 0.1–600 µg.mL−1
abstractGer	A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albuminuria in urine samples. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration of human serum albumin in urine. The limit of detection of the developed platform is 0.05 µg.mL−1 and the detection range is 0.1–600 µg.mL−1, which covers the albuminuria concentration range present in normal human urine and the urine of the patient with chronic kidney disease. This approach can be modified to measure albuminuria using a high-throughput quantification platform and portable point of care testing. In addition, the production cost for one reaction is cheaper than those for the standard automated method. Therefore, this aptasensor has significant potential for commercialization and public use. • Our protocol is customized by using the fluorescence quenching property of graphene oxide and specific binding property of human serum albumin aptamer to detect human serum albumin in urine sample • The limit of detection of our developed platform is 0.05 µg.mL−1 • The detection range of our aptasensor is 0.1–600 µg.mL−1
abstract_unstemmed	A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albuminuria in urine samples. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration of human serum albumin in urine. The limit of detection of the developed platform is 0.05 µg.mL−1 and the detection range is 0.1–600 µg.mL−1, which covers the albuminuria concentration range present in normal human urine and the urine of the patient with chronic kidney disease. This approach can be modified to measure albuminuria using a high-throughput quantification platform and portable point of care testing. In addition, the production cost for one reaction is cheaper than those for the standard automated method. Therefore, this aptasensor has significant potential for commercialization and public use. • Our protocol is customized by using the fluorescence quenching property of graphene oxide and specific binding property of human serum albumin aptamer to detect human serum albumin in urine sample • The limit of detection of our developed platform is 0.05 µg.mL−1 • The detection range of our aptasensor is 0.1–600 µg.mL−1
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title_short	Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor
url	https://doi.org/10.1016/j.mex.2020.101114 https://doaj.org/article/d604f2bf1f5b4a26baeeec4d18b6858f http://www.sciencedirect.com/science/article/pii/S2215016120303344 https://doaj.org/toc/2215-0161
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author2Str	Chayachon Apiwat Khoonsake Segkhoonthod Kiatnida Treerattrakoon Preedee Pinpradup Nuankanya Sathirapongsasuti Prapasiri Pongprayoon Patraporn Luksirikul Patcharee Isarankura-Na-Ayudhya Deanpen Japrung
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The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration of human serum albumin in urine. The limit of detection of the developed platform is 0.05 µg.mL−1 and the detection range is 0.1–600 µg.mL−1, which covers the albuminuria concentration range present in normal human urine and the urine of the patient with chronic kidney disease. This approach can be modified to measure albuminuria using a high-throughput quantification platform and portable point of care testing. In addition, the production cost for one reaction is cheaper than those for the standard automated method. 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Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor

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