In vitro Culture with Cytokines Provides a Tool to Assess the Effector Functions of ILC2s in Peripheral Blood in Asthma
Li Y Drake,1,2 Kathleen R Bartemes,1,3 Kay A Bachman,1 John B Hagan,1 Hirohito Kita1,4 1Division of Allergic Diseases and Department of Medicine, Mayo Clinic, Rochester, MN, USA; 2Department of Anesthesiology and Perioperative Medicine, Mayo Clinic, Rochester, MN, USA; 3Department of Otorhinolaryngo...
Ausführliche Beschreibung
Autor*in: |
Drake LY [verfasserIn] Bartemes KR [verfasserIn] Bachman KA [verfasserIn] Hagan JB [verfasserIn] Kita H [verfasserIn] |
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Englisch |
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2021 |
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In: Journal of Asthma and Allergy - Dove Medical Press, 2009, (2021), Seite 13-22 |
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year:2021 ; pages:13-22 |
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DOAJ078426405 |
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520 | |a Li Y Drake,1,2 Kathleen R Bartemes,1,3 Kay A Bachman,1 John B Hagan,1 Hirohito Kita1,4 1Division of Allergic Diseases and Department of Medicine, Mayo Clinic, Rochester, MN, USA; 2Department of Anesthesiology and Perioperative Medicine, Mayo Clinic, Rochester, MN, USA; 3Department of Otorhinolaryngology, Mayo Clinic, Rochester, MN, USA; 4Division of Allergy, Asthma, and Clinical Immunology and Department of Medicine, Mayo Clinic Arizona, Scottsdale, AZ, USACorrespondence: Hirohito KitaDivision of Allergy, Asthma, and Clinical Immunology and Department of Medicine, Mayo Clinic Arizona, Scottsdale, AZ 85259, USATel +1 480 301 9616Fax +1 480 301 7017Email Kita.hirohitomayo.eduBackground: Group 2 innate lymphoid cells (ILC2s) play crucial roles in type 2 immunity and asthma development. While ILC2s are resident in mucosal tissues, they also circulate in peripheral blood. It remains controversial whether ILC2s are increased in the peripheral blood of patients with asthma.Purpose: The goal of this project was to study the effector functions of ILC2s in peripheral blood samples by in vitro culture with cytokines.Patients and Methods: Peripheral blood mononuclear cells (PBMCs) were collected from 11 adult patients with mild asthma and 12 healthy control subjects. The number of peripheral blood ILC2s in PBMCs was analyzed by flow cytometry. PBMCs were cultured with IL-33 and IL-25 without any antigens, and the amounts of type 2 cytokines in cell-free supernatants were analyzed by ELISA. In selected experiments, production of cytokines by ILC2s was analyzed by intracellular cytokine staining and flow cytometry.Results: In response to either IL-33 or IL-25 stimulation, PBMCs from patients with mild asthma produced larger amounts of IL-5 and IL-13 than PBMCs from healthy control subjects. However, ILC2 numbers or proportions were not significantly different between these two groups. Flow cytometric analysis confirmed production of IL-5 by ILCs when stimulated with IL-33.Conclusion: In vitro culture of PBMCs with a cocktail of cytokines, such as either IL-33 or IL-25 plus IL-2, may provide a valuable tool to assess the effector functions of ILC2s and may serve as a biomarker for human asthma.Keywords: IL-33, IL-25, IL-5, IL-13 | ||
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(DE-627)DOAJ078426405 (DE-599)DOAJ0b3352696efb45b8bc8ff6e31f6c191e DE-627 ger DE-627 rakwb eng RC581-607 Drake LY verfasserin aut In vitro Culture with Cytokines Provides a Tool to Assess the Effector Functions of ILC2s in Peripheral Blood in Asthma 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Li Y Drake,1,2 Kathleen R Bartemes,1,3 Kay A Bachman,1 John B Hagan,1 Hirohito Kita1,4 1Division of Allergic Diseases and Department of Medicine, Mayo Clinic, Rochester, MN, USA; 2Department of Anesthesiology and Perioperative Medicine, Mayo Clinic, Rochester, MN, USA; 3Department of Otorhinolaryngology, Mayo Clinic, Rochester, MN, USA; 4Division of Allergy, Asthma, and Clinical Immunology and Department of Medicine, Mayo Clinic Arizona, Scottsdale, AZ, USACorrespondence: Hirohito KitaDivision of Allergy, Asthma, and Clinical Immunology and Department of Medicine, Mayo Clinic Arizona, Scottsdale, AZ 85259, USATel +1 480 301 9616Fax +1 480 301 7017Email Kita.hirohitomayo.eduBackground: Group 2 innate lymphoid cells (ILC2s) play crucial roles in type 2 immunity and asthma development. While ILC2s are resident in mucosal tissues, they also circulate in peripheral blood. It remains controversial whether ILC2s are increased in the peripheral blood of patients with asthma.Purpose: The goal of this project was to study the effector functions of ILC2s in peripheral blood samples by in vitro culture with cytokines.Patients and Methods: Peripheral blood mononuclear cells (PBMCs) were collected from 11 adult patients with mild asthma and 12 healthy control subjects. The number of peripheral blood ILC2s in PBMCs was analyzed by flow cytometry. PBMCs were cultured with IL-33 and IL-25 without any antigens, and the amounts of type 2 cytokines in cell-free supernatants were analyzed by ELISA. In selected experiments, production of cytokines by ILC2s was analyzed by intracellular cytokine staining and flow cytometry.Results: In response to either IL-33 or IL-25 stimulation, PBMCs from patients with mild asthma produced larger amounts of IL-5 and IL-13 than PBMCs from healthy control subjects. However, ILC2 numbers or proportions were not significantly different between these two groups. Flow cytometric analysis confirmed production of IL-5 by ILCs when stimulated with IL-33.Conclusion: In vitro culture of PBMCs with a cocktail of cytokines, such as either IL-33 or IL-25 plus IL-2, may provide a valuable tool to assess the effector functions of ILC2s and may serve as a biomarker for human asthma.Keywords: IL-33, IL-25, IL-5, IL-13 il-33 il-25 il-5 il-13 Immunologic diseases. Allergy Bartemes KR verfasserin aut Bachman KA verfasserin aut Hagan JB verfasserin aut Kita H verfasserin aut In Journal of Asthma and Allergy Dove Medical Press, 2009 (2021), Seite 13-22 (DE-627)60030616X (DE-600)2494877-9 11786965 nnns year:2021 pages:13-22 https://doaj.org/article/0b3352696efb45b8bc8ff6e31f6c191e kostenfrei https://www.dovepress.com/in-vitro-culture-with-cytokines-provides-a-tool-to-assess-the-effector-peer-reviewed-article-JAA kostenfrei https://doaj.org/toc/1178-6965 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2021 13-22 |
spelling |
(DE-627)DOAJ078426405 (DE-599)DOAJ0b3352696efb45b8bc8ff6e31f6c191e DE-627 ger DE-627 rakwb eng RC581-607 Drake LY verfasserin aut In vitro Culture with Cytokines Provides a Tool to Assess the Effector Functions of ILC2s in Peripheral Blood in Asthma 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Li Y Drake,1,2 Kathleen R Bartemes,1,3 Kay A Bachman,1 John B Hagan,1 Hirohito Kita1,4 1Division of Allergic Diseases and Department of Medicine, Mayo Clinic, Rochester, MN, USA; 2Department of Anesthesiology and Perioperative Medicine, Mayo Clinic, Rochester, MN, USA; 3Department of Otorhinolaryngology, Mayo Clinic, Rochester, MN, USA; 4Division of Allergy, Asthma, and Clinical Immunology and Department of Medicine, Mayo Clinic Arizona, Scottsdale, AZ, USACorrespondence: Hirohito KitaDivision of Allergy, Asthma, and Clinical Immunology and Department of Medicine, Mayo Clinic Arizona, Scottsdale, AZ 85259, USATel +1 480 301 9616Fax +1 480 301 7017Email Kita.hirohitomayo.eduBackground: Group 2 innate lymphoid cells (ILC2s) play crucial roles in type 2 immunity and asthma development. While ILC2s are resident in mucosal tissues, they also circulate in peripheral blood. It remains controversial whether ILC2s are increased in the peripheral blood of patients with asthma.Purpose: The goal of this project was to study the effector functions of ILC2s in peripheral blood samples by in vitro culture with cytokines.Patients and Methods: Peripheral blood mononuclear cells (PBMCs) were collected from 11 adult patients with mild asthma and 12 healthy control subjects. The number of peripheral blood ILC2s in PBMCs was analyzed by flow cytometry. PBMCs were cultured with IL-33 and IL-25 without any antigens, and the amounts of type 2 cytokines in cell-free supernatants were analyzed by ELISA. In selected experiments, production of cytokines by ILC2s was analyzed by intracellular cytokine staining and flow cytometry.Results: In response to either IL-33 or IL-25 stimulation, PBMCs from patients with mild asthma produced larger amounts of IL-5 and IL-13 than PBMCs from healthy control subjects. However, ILC2 numbers or proportions were not significantly different between these two groups. Flow cytometric analysis confirmed production of IL-5 by ILCs when stimulated with IL-33.Conclusion: In vitro culture of PBMCs with a cocktail of cytokines, such as either IL-33 or IL-25 plus IL-2, may provide a valuable tool to assess the effector functions of ILC2s and may serve as a biomarker for human asthma.Keywords: IL-33, IL-25, IL-5, IL-13 il-33 il-25 il-5 il-13 Immunologic diseases. Allergy Bartemes KR verfasserin aut Bachman KA verfasserin aut Hagan JB verfasserin aut Kita H verfasserin aut In Journal of Asthma and Allergy Dove Medical Press, 2009 (2021), Seite 13-22 (DE-627)60030616X (DE-600)2494877-9 11786965 nnns year:2021 pages:13-22 https://doaj.org/article/0b3352696efb45b8bc8ff6e31f6c191e kostenfrei https://www.dovepress.com/in-vitro-culture-with-cytokines-provides-a-tool-to-assess-the-effector-peer-reviewed-article-JAA kostenfrei https://doaj.org/toc/1178-6965 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2021 13-22 |
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(DE-627)DOAJ078426405 (DE-599)DOAJ0b3352696efb45b8bc8ff6e31f6c191e DE-627 ger DE-627 rakwb eng RC581-607 Drake LY verfasserin aut In vitro Culture with Cytokines Provides a Tool to Assess the Effector Functions of ILC2s in Peripheral Blood in Asthma 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Li Y Drake,1,2 Kathleen R Bartemes,1,3 Kay A Bachman,1 John B Hagan,1 Hirohito Kita1,4 1Division of Allergic Diseases and Department of Medicine, Mayo Clinic, Rochester, MN, USA; 2Department of Anesthesiology and Perioperative Medicine, Mayo Clinic, Rochester, MN, USA; 3Department of Otorhinolaryngology, Mayo Clinic, Rochester, MN, USA; 4Division of Allergy, Asthma, and Clinical Immunology and Department of Medicine, Mayo Clinic Arizona, Scottsdale, AZ, USACorrespondence: Hirohito KitaDivision of Allergy, Asthma, and Clinical Immunology and Department of Medicine, Mayo Clinic Arizona, Scottsdale, AZ 85259, USATel +1 480 301 9616Fax +1 480 301 7017Email Kita.hirohitomayo.eduBackground: Group 2 innate lymphoid cells (ILC2s) play crucial roles in type 2 immunity and asthma development. While ILC2s are resident in mucosal tissues, they also circulate in peripheral blood. It remains controversial whether ILC2s are increased in the peripheral blood of patients with asthma.Purpose: The goal of this project was to study the effector functions of ILC2s in peripheral blood samples by in vitro culture with cytokines.Patients and Methods: Peripheral blood mononuclear cells (PBMCs) were collected from 11 adult patients with mild asthma and 12 healthy control subjects. The number of peripheral blood ILC2s in PBMCs was analyzed by flow cytometry. PBMCs were cultured with IL-33 and IL-25 without any antigens, and the amounts of type 2 cytokines in cell-free supernatants were analyzed by ELISA. In selected experiments, production of cytokines by ILC2s was analyzed by intracellular cytokine staining and flow cytometry.Results: In response to either IL-33 or IL-25 stimulation, PBMCs from patients with mild asthma produced larger amounts of IL-5 and IL-13 than PBMCs from healthy control subjects. However, ILC2 numbers or proportions were not significantly different between these two groups. Flow cytometric analysis confirmed production of IL-5 by ILCs when stimulated with IL-33.Conclusion: In vitro culture of PBMCs with a cocktail of cytokines, such as either IL-33 or IL-25 plus IL-2, may provide a valuable tool to assess the effector functions of ILC2s and may serve as a biomarker for human asthma.Keywords: IL-33, IL-25, IL-5, IL-13 il-33 il-25 il-5 il-13 Immunologic diseases. Allergy Bartemes KR verfasserin aut Bachman KA verfasserin aut Hagan JB verfasserin aut Kita H verfasserin aut In Journal of Asthma and Allergy Dove Medical Press, 2009 (2021), Seite 13-22 (DE-627)60030616X (DE-600)2494877-9 11786965 nnns year:2021 pages:13-22 https://doaj.org/article/0b3352696efb45b8bc8ff6e31f6c191e kostenfrei https://www.dovepress.com/in-vitro-culture-with-cytokines-provides-a-tool-to-assess-the-effector-peer-reviewed-article-JAA kostenfrei https://doaj.org/toc/1178-6965 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2021 13-22 |
allfieldsGer |
(DE-627)DOAJ078426405 (DE-599)DOAJ0b3352696efb45b8bc8ff6e31f6c191e DE-627 ger DE-627 rakwb eng RC581-607 Drake LY verfasserin aut In vitro Culture with Cytokines Provides a Tool to Assess the Effector Functions of ILC2s in Peripheral Blood in Asthma 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Li Y Drake,1,2 Kathleen R Bartemes,1,3 Kay A Bachman,1 John B Hagan,1 Hirohito Kita1,4 1Division of Allergic Diseases and Department of Medicine, Mayo Clinic, Rochester, MN, USA; 2Department of Anesthesiology and Perioperative Medicine, Mayo Clinic, Rochester, MN, USA; 3Department of Otorhinolaryngology, Mayo Clinic, Rochester, MN, USA; 4Division of Allergy, Asthma, and Clinical Immunology and Department of Medicine, Mayo Clinic Arizona, Scottsdale, AZ, USACorrespondence: Hirohito KitaDivision of Allergy, Asthma, and Clinical Immunology and Department of Medicine, Mayo Clinic Arizona, Scottsdale, AZ 85259, USATel +1 480 301 9616Fax +1 480 301 7017Email Kita.hirohitomayo.eduBackground: Group 2 innate lymphoid cells (ILC2s) play crucial roles in type 2 immunity and asthma development. While ILC2s are resident in mucosal tissues, they also circulate in peripheral blood. It remains controversial whether ILC2s are increased in the peripheral blood of patients with asthma.Purpose: The goal of this project was to study the effector functions of ILC2s in peripheral blood samples by in vitro culture with cytokines.Patients and Methods: Peripheral blood mononuclear cells (PBMCs) were collected from 11 adult patients with mild asthma and 12 healthy control subjects. The number of peripheral blood ILC2s in PBMCs was analyzed by flow cytometry. PBMCs were cultured with IL-33 and IL-25 without any antigens, and the amounts of type 2 cytokines in cell-free supernatants were analyzed by ELISA. In selected experiments, production of cytokines by ILC2s was analyzed by intracellular cytokine staining and flow cytometry.Results: In response to either IL-33 or IL-25 stimulation, PBMCs from patients with mild asthma produced larger amounts of IL-5 and IL-13 than PBMCs from healthy control subjects. However, ILC2 numbers or proportions were not significantly different between these two groups. Flow cytometric analysis confirmed production of IL-5 by ILCs when stimulated with IL-33.Conclusion: In vitro culture of PBMCs with a cocktail of cytokines, such as either IL-33 or IL-25 plus IL-2, may provide a valuable tool to assess the effector functions of ILC2s and may serve as a biomarker for human asthma.Keywords: IL-33, IL-25, IL-5, IL-13 il-33 il-25 il-5 il-13 Immunologic diseases. Allergy Bartemes KR verfasserin aut Bachman KA verfasserin aut Hagan JB verfasserin aut Kita H verfasserin aut In Journal of Asthma and Allergy Dove Medical Press, 2009 (2021), Seite 13-22 (DE-627)60030616X (DE-600)2494877-9 11786965 nnns year:2021 pages:13-22 https://doaj.org/article/0b3352696efb45b8bc8ff6e31f6c191e kostenfrei https://www.dovepress.com/in-vitro-culture-with-cytokines-provides-a-tool-to-assess-the-effector-peer-reviewed-article-JAA kostenfrei https://doaj.org/toc/1178-6965 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2021 13-22 |
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(DE-627)DOAJ078426405 (DE-599)DOAJ0b3352696efb45b8bc8ff6e31f6c191e DE-627 ger DE-627 rakwb eng RC581-607 Drake LY verfasserin aut In vitro Culture with Cytokines Provides a Tool to Assess the Effector Functions of ILC2s in Peripheral Blood in Asthma 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Li Y Drake,1,2 Kathleen R Bartemes,1,3 Kay A Bachman,1 John B Hagan,1 Hirohito Kita1,4 1Division of Allergic Diseases and Department of Medicine, Mayo Clinic, Rochester, MN, USA; 2Department of Anesthesiology and Perioperative Medicine, Mayo Clinic, Rochester, MN, USA; 3Department of Otorhinolaryngology, Mayo Clinic, Rochester, MN, USA; 4Division of Allergy, Asthma, and Clinical Immunology and Department of Medicine, Mayo Clinic Arizona, Scottsdale, AZ, USACorrespondence: Hirohito KitaDivision of Allergy, Asthma, and Clinical Immunology and Department of Medicine, Mayo Clinic Arizona, Scottsdale, AZ 85259, USATel +1 480 301 9616Fax +1 480 301 7017Email Kita.hirohitomayo.eduBackground: Group 2 innate lymphoid cells (ILC2s) play crucial roles in type 2 immunity and asthma development. While ILC2s are resident in mucosal tissues, they also circulate in peripheral blood. It remains controversial whether ILC2s are increased in the peripheral blood of patients with asthma.Purpose: The goal of this project was to study the effector functions of ILC2s in peripheral blood samples by in vitro culture with cytokines.Patients and Methods: Peripheral blood mononuclear cells (PBMCs) were collected from 11 adult patients with mild asthma and 12 healthy control subjects. The number of peripheral blood ILC2s in PBMCs was analyzed by flow cytometry. PBMCs were cultured with IL-33 and IL-25 without any antigens, and the amounts of type 2 cytokines in cell-free supernatants were analyzed by ELISA. In selected experiments, production of cytokines by ILC2s was analyzed by intracellular cytokine staining and flow cytometry.Results: In response to either IL-33 or IL-25 stimulation, PBMCs from patients with mild asthma produced larger amounts of IL-5 and IL-13 than PBMCs from healthy control subjects. However, ILC2 numbers or proportions were not significantly different between these two groups. Flow cytometric analysis confirmed production of IL-5 by ILCs when stimulated with IL-33.Conclusion: In vitro culture of PBMCs with a cocktail of cytokines, such as either IL-33 or IL-25 plus IL-2, may provide a valuable tool to assess the effector functions of ILC2s and may serve as a biomarker for human asthma.Keywords: IL-33, IL-25, IL-5, IL-13 il-33 il-25 il-5 il-13 Immunologic diseases. Allergy Bartemes KR verfasserin aut Bachman KA verfasserin aut Hagan JB verfasserin aut Kita H verfasserin aut In Journal of Asthma and Allergy Dove Medical Press, 2009 (2021), Seite 13-22 (DE-627)60030616X (DE-600)2494877-9 11786965 nnns year:2021 pages:13-22 https://doaj.org/article/0b3352696efb45b8bc8ff6e31f6c191e kostenfrei https://www.dovepress.com/in-vitro-culture-with-cytokines-provides-a-tool-to-assess-the-effector-peer-reviewed-article-JAA kostenfrei https://doaj.org/toc/1178-6965 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2021 13-22 |
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Li Y Drake,1,2 Kathleen R Bartemes,1,3 Kay A Bachman,1 John B Hagan,1 Hirohito Kita1,4 1Division of Allergic Diseases and Department of Medicine, Mayo Clinic, Rochester, MN, USA; 2Department of Anesthesiology and Perioperative Medicine, Mayo Clinic, Rochester, MN, USA; 3Department of Otorhinolaryngology, Mayo Clinic, Rochester, MN, USA; 4Division of Allergy, Asthma, and Clinical Immunology and Department of Medicine, Mayo Clinic Arizona, Scottsdale, AZ, USACorrespondence: Hirohito KitaDivision of Allergy, Asthma, and Clinical Immunology and Department of Medicine, Mayo Clinic Arizona, Scottsdale, AZ 85259, USATel +1 480 301 9616Fax +1 480 301 7017Email Kita.hirohitomayo.eduBackground: Group 2 innate lymphoid cells (ILC2s) play crucial roles in type 2 immunity and asthma development. While ILC2s are resident in mucosal tissues, they also circulate in peripheral blood. It remains controversial whether ILC2s are increased in the peripheral blood of patients with asthma.Purpose: The goal of this project was to study the effector functions of ILC2s in peripheral blood samples by in vitro culture with cytokines.Patients and Methods: Peripheral blood mononuclear cells (PBMCs) were collected from 11 adult patients with mild asthma and 12 healthy control subjects. The number of peripheral blood ILC2s in PBMCs was analyzed by flow cytometry. PBMCs were cultured with IL-33 and IL-25 without any antigens, and the amounts of type 2 cytokines in cell-free supernatants were analyzed by ELISA. In selected experiments, production of cytokines by ILC2s was analyzed by intracellular cytokine staining and flow cytometry.Results: In response to either IL-33 or IL-25 stimulation, PBMCs from patients with mild asthma produced larger amounts of IL-5 and IL-13 than PBMCs from healthy control subjects. However, ILC2 numbers or proportions were not significantly different between these two groups. Flow cytometric analysis confirmed production of IL-5 by ILCs when stimulated with IL-33.Conclusion: In vitro culture of PBMCs with a cocktail of cytokines, such as either IL-33 or IL-25 plus IL-2, may provide a valuable tool to assess the effector functions of ILC2s and may serve as a biomarker for human asthma.Keywords: IL-33, IL-25, IL-5, IL-13 |
abstractGer |
Li Y Drake,1,2 Kathleen R Bartemes,1,3 Kay A Bachman,1 John B Hagan,1 Hirohito Kita1,4 1Division of Allergic Diseases and Department of Medicine, Mayo Clinic, Rochester, MN, USA; 2Department of Anesthesiology and Perioperative Medicine, Mayo Clinic, Rochester, MN, USA; 3Department of Otorhinolaryngology, Mayo Clinic, Rochester, MN, USA; 4Division of Allergy, Asthma, and Clinical Immunology and Department of Medicine, Mayo Clinic Arizona, Scottsdale, AZ, USACorrespondence: Hirohito KitaDivision of Allergy, Asthma, and Clinical Immunology and Department of Medicine, Mayo Clinic Arizona, Scottsdale, AZ 85259, USATel +1 480 301 9616Fax +1 480 301 7017Email Kita.hirohitomayo.eduBackground: Group 2 innate lymphoid cells (ILC2s) play crucial roles in type 2 immunity and asthma development. While ILC2s are resident in mucosal tissues, they also circulate in peripheral blood. It remains controversial whether ILC2s are increased in the peripheral blood of patients with asthma.Purpose: The goal of this project was to study the effector functions of ILC2s in peripheral blood samples by in vitro culture with cytokines.Patients and Methods: Peripheral blood mononuclear cells (PBMCs) were collected from 11 adult patients with mild asthma and 12 healthy control subjects. The number of peripheral blood ILC2s in PBMCs was analyzed by flow cytometry. PBMCs were cultured with IL-33 and IL-25 without any antigens, and the amounts of type 2 cytokines in cell-free supernatants were analyzed by ELISA. In selected experiments, production of cytokines by ILC2s was analyzed by intracellular cytokine staining and flow cytometry.Results: In response to either IL-33 or IL-25 stimulation, PBMCs from patients with mild asthma produced larger amounts of IL-5 and IL-13 than PBMCs from healthy control subjects. However, ILC2 numbers or proportions were not significantly different between these two groups. Flow cytometric analysis confirmed production of IL-5 by ILCs when stimulated with IL-33.Conclusion: In vitro culture of PBMCs with a cocktail of cytokines, such as either IL-33 or IL-25 plus IL-2, may provide a valuable tool to assess the effector functions of ILC2s and may serve as a biomarker for human asthma.Keywords: IL-33, IL-25, IL-5, IL-13 |
abstract_unstemmed |
Li Y Drake,1,2 Kathleen R Bartemes,1,3 Kay A Bachman,1 John B Hagan,1 Hirohito Kita1,4 1Division of Allergic Diseases and Department of Medicine, Mayo Clinic, Rochester, MN, USA; 2Department of Anesthesiology and Perioperative Medicine, Mayo Clinic, Rochester, MN, USA; 3Department of Otorhinolaryngology, Mayo Clinic, Rochester, MN, USA; 4Division of Allergy, Asthma, and Clinical Immunology and Department of Medicine, Mayo Clinic Arizona, Scottsdale, AZ, USACorrespondence: Hirohito KitaDivision of Allergy, Asthma, and Clinical Immunology and Department of Medicine, Mayo Clinic Arizona, Scottsdale, AZ 85259, USATel +1 480 301 9616Fax +1 480 301 7017Email Kita.hirohitomayo.eduBackground: Group 2 innate lymphoid cells (ILC2s) play crucial roles in type 2 immunity and asthma development. While ILC2s are resident in mucosal tissues, they also circulate in peripheral blood. It remains controversial whether ILC2s are increased in the peripheral blood of patients with asthma.Purpose: The goal of this project was to study the effector functions of ILC2s in peripheral blood samples by in vitro culture with cytokines.Patients and Methods: Peripheral blood mononuclear cells (PBMCs) were collected from 11 adult patients with mild asthma and 12 healthy control subjects. The number of peripheral blood ILC2s in PBMCs was analyzed by flow cytometry. PBMCs were cultured with IL-33 and IL-25 without any antigens, and the amounts of type 2 cytokines in cell-free supernatants were analyzed by ELISA. In selected experiments, production of cytokines by ILC2s was analyzed by intracellular cytokine staining and flow cytometry.Results: In response to either IL-33 or IL-25 stimulation, PBMCs from patients with mild asthma produced larger amounts of IL-5 and IL-13 than PBMCs from healthy control subjects. However, ILC2 numbers or proportions were not significantly different between these two groups. Flow cytometric analysis confirmed production of IL-5 by ILCs when stimulated with IL-33.Conclusion: In vitro culture of PBMCs with a cocktail of cytokines, such as either IL-33 or IL-25 plus IL-2, may provide a valuable tool to assess the effector functions of ILC2s and may serve as a biomarker for human asthma.Keywords: IL-33, IL-25, IL-5, IL-13 |
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title_short |
In vitro Culture with Cytokines Provides a Tool to Assess the Effector Functions of ILC2s in Peripheral Blood in Asthma |
url |
https://doaj.org/article/0b3352696efb45b8bc8ff6e31f6c191e https://www.dovepress.com/in-vitro-culture-with-cytokines-provides-a-tool-to-assess-the-effector-peer-reviewed-article-JAA https://doaj.org/toc/1178-6965 |
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