Emerging Therapeutic Activity of <i<Davallia formosana</i< on Prostate Cancer Cells through Coordinated Blockade of Lipogenesis and Androgen Receptor Expression

Background: Prostate cancer (PCa) is the most prevalent malignancy diagnosed in men in Western countries. There is currently no effective therapy for advanced PCa aggressiveness, including castration-resistant progression. The aim of this study is to evaluate the potential efficacy and determine the...
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Autor*in:	Po-Fan Hsieh [verfasserIn] Wen-Ping Jiang [verfasserIn] Shih-Yin Huang [verfasserIn] Praveenkumar Basavaraj [verfasserIn] Jin-Bin Wu [verfasserIn] Hui-Ya Ho [verfasserIn] Guan-Jhong Huang [verfasserIn] Wen-Chin Huang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	anti-prostate cancer efficacy SREBP-1/FASN/lipogenesis AR

Übergeordnetes Werk:	In: Cancers - MDPI AG, 2010, 12(2020), 4, p 914
Übergeordnetes Werk:	volume:12 ; year:2020 ; number:4, p 914

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/cancers12040914

Katalog-ID:	DOAJ078439477

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520			\|a Background: Prostate cancer (PCa) is the most prevalent malignancy diagnosed in men in Western countries. There is currently no effective therapy for advanced PCa aggressiveness, including castration-resistant progression. The aim of this study is to evaluate the potential efficacy and determine the molecular basis of <i<Davallia formosana</i< (DF) in PCa. Methods: LNCaP (androgen-sensitive) and C4-2 (androgen-insensitive/castration-resistant) PCa cells were utilized in this study. An MTT-based method, a wound healing assay, and the transwell method were performed to evaluate cell proliferation, migration, and invasion. Intracellular fatty acid levels and lipid droplet accumulation were analyzed to determine lipogenesis. Moreover, apoptotic assays and in vivo experiments were conducted. Results: DF ethanol extract (DFE) suppressed proliferation, migration, and invasion in PCa cells. DFE attenuated lipogenesis through inhibition of the expression of sterol regulatory element-binding protein-1 (SREBP-1) and fatty acid synthase (FASN). Moreover, DFE decreased androgen receptor (AR) and prostate-specific antigen (PSA) expression in PCa cells. We further showed the potent therapeutic activity of DFE by repressing the growth and leading to apoptosis of subcutaneous C4-2 tumors in a xenograft mouse model. Conclusions: These data provide a new molecular basis of DFE in PCa cells, and co-targeting SREBP-1/FASN/lipogenesis and the AR axis by DFE could be employed as a novel and promising strategy for the treatment of PCa.
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allfields	10.3390/cancers12040914 doi (DE-627)DOAJ078439477 (DE-599)DOAJdeb48556692b4ddd93df265db9e9ae23 DE-627 ger DE-627 rakwb eng RC254-282 Po-Fan Hsieh verfasserin aut Emerging Therapeutic Activity of <i<Davallia formosana</i< on Prostate Cancer Cells through Coordinated Blockade of Lipogenesis and Androgen Receptor Expression 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Prostate cancer (PCa) is the most prevalent malignancy diagnosed in men in Western countries. There is currently no effective therapy for advanced PCa aggressiveness, including castration-resistant progression. The aim of this study is to evaluate the potential efficacy and determine the molecular basis of <i<Davallia formosana</i< (DF) in PCa. Methods: LNCaP (androgen-sensitive) and C4-2 (androgen-insensitive/castration-resistant) PCa cells were utilized in this study. An MTT-based method, a wound healing assay, and the transwell method were performed to evaluate cell proliferation, migration, and invasion. Intracellular fatty acid levels and lipid droplet accumulation were analyzed to determine lipogenesis. Moreover, apoptotic assays and in vivo experiments were conducted. Results: DF ethanol extract (DFE) suppressed proliferation, migration, and invasion in PCa cells. DFE attenuated lipogenesis through inhibition of the expression of sterol regulatory element-binding protein-1 (SREBP-1) and fatty acid synthase (FASN). Moreover, DFE decreased androgen receptor (AR) and prostate-specific antigen (PSA) expression in PCa cells. We further showed the potent therapeutic activity of DFE by repressing the growth and leading to apoptosis of subcutaneous C4-2 tumors in a xenograft mouse model. Conclusions: These data provide a new molecular basis of DFE in PCa cells, and co-targeting SREBP-1/FASN/lipogenesis and the AR axis by DFE could be employed as a novel and promising strategy for the treatment of PCa. <i<Davallia formosana</i< anti-prostate cancer efficacy SREBP-1/FASN/lipogenesis AR Neoplasms. Tumors. Oncology. Including cancer and carcinogens Wen-Ping Jiang verfasserin aut Shih-Yin Huang verfasserin aut Praveenkumar Basavaraj verfasserin aut Jin-Bin Wu verfasserin aut Hui-Ya Ho verfasserin aut Guan-Jhong Huang verfasserin aut Wen-Chin Huang verfasserin aut In Cancers MDPI AG, 2010 12(2020), 4, p 914 (DE-627)614095670 (DE-600)2527080-1 20726694 nnns volume:12 year:2020 number:4, p 914 https://doi.org/10.3390/cancers12040914 kostenfrei https://doaj.org/article/deb48556692b4ddd93df265db9e9ae23 kostenfrei https://www.mdpi.com/2072-6694/12/4/914 kostenfrei https://doaj.org/toc/2072-6694 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 4, p 914
spelling	10.3390/cancers12040914 doi (DE-627)DOAJ078439477 (DE-599)DOAJdeb48556692b4ddd93df265db9e9ae23 DE-627 ger DE-627 rakwb eng RC254-282 Po-Fan Hsieh verfasserin aut Emerging Therapeutic Activity of <i<Davallia formosana</i< on Prostate Cancer Cells through Coordinated Blockade of Lipogenesis and Androgen Receptor Expression 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Prostate cancer (PCa) is the most prevalent malignancy diagnosed in men in Western countries. There is currently no effective therapy for advanced PCa aggressiveness, including castration-resistant progression. The aim of this study is to evaluate the potential efficacy and determine the molecular basis of <i<Davallia formosana</i< (DF) in PCa. Methods: LNCaP (androgen-sensitive) and C4-2 (androgen-insensitive/castration-resistant) PCa cells were utilized in this study. An MTT-based method, a wound healing assay, and the transwell method were performed to evaluate cell proliferation, migration, and invasion. Intracellular fatty acid levels and lipid droplet accumulation were analyzed to determine lipogenesis. Moreover, apoptotic assays and in vivo experiments were conducted. Results: DF ethanol extract (DFE) suppressed proliferation, migration, and invasion in PCa cells. DFE attenuated lipogenesis through inhibition of the expression of sterol regulatory element-binding protein-1 (SREBP-1) and fatty acid synthase (FASN). Moreover, DFE decreased androgen receptor (AR) and prostate-specific antigen (PSA) expression in PCa cells. We further showed the potent therapeutic activity of DFE by repressing the growth and leading to apoptosis of subcutaneous C4-2 tumors in a xenograft mouse model. Conclusions: These data provide a new molecular basis of DFE in PCa cells, and co-targeting SREBP-1/FASN/lipogenesis and the AR axis by DFE could be employed as a novel and promising strategy for the treatment of PCa. <i<Davallia formosana</i< anti-prostate cancer efficacy SREBP-1/FASN/lipogenesis AR Neoplasms. Tumors. Oncology. Including cancer and carcinogens Wen-Ping Jiang verfasserin aut Shih-Yin Huang verfasserin aut Praveenkumar Basavaraj verfasserin aut Jin-Bin Wu verfasserin aut Hui-Ya Ho verfasserin aut Guan-Jhong Huang verfasserin aut Wen-Chin Huang verfasserin aut In Cancers MDPI AG, 2010 12(2020), 4, p 914 (DE-627)614095670 (DE-600)2527080-1 20726694 nnns volume:12 year:2020 number:4, p 914 https://doi.org/10.3390/cancers12040914 kostenfrei https://doaj.org/article/deb48556692b4ddd93df265db9e9ae23 kostenfrei https://www.mdpi.com/2072-6694/12/4/914 kostenfrei https://doaj.org/toc/2072-6694 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 4, p 914
allfields_unstemmed	10.3390/cancers12040914 doi (DE-627)DOAJ078439477 (DE-599)DOAJdeb48556692b4ddd93df265db9e9ae23 DE-627 ger DE-627 rakwb eng RC254-282 Po-Fan Hsieh verfasserin aut Emerging Therapeutic Activity of <i<Davallia formosana</i< on Prostate Cancer Cells through Coordinated Blockade of Lipogenesis and Androgen Receptor Expression 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Prostate cancer (PCa) is the most prevalent malignancy diagnosed in men in Western countries. There is currently no effective therapy for advanced PCa aggressiveness, including castration-resistant progression. The aim of this study is to evaluate the potential efficacy and determine the molecular basis of <i<Davallia formosana</i< (DF) in PCa. Methods: LNCaP (androgen-sensitive) and C4-2 (androgen-insensitive/castration-resistant) PCa cells were utilized in this study. An MTT-based method, a wound healing assay, and the transwell method were performed to evaluate cell proliferation, migration, and invasion. Intracellular fatty acid levels and lipid droplet accumulation were analyzed to determine lipogenesis. Moreover, apoptotic assays and in vivo experiments were conducted. Results: DF ethanol extract (DFE) suppressed proliferation, migration, and invasion in PCa cells. DFE attenuated lipogenesis through inhibition of the expression of sterol regulatory element-binding protein-1 (SREBP-1) and fatty acid synthase (FASN). Moreover, DFE decreased androgen receptor (AR) and prostate-specific antigen (PSA) expression in PCa cells. We further showed the potent therapeutic activity of DFE by repressing the growth and leading to apoptosis of subcutaneous C4-2 tumors in a xenograft mouse model. Conclusions: These data provide a new molecular basis of DFE in PCa cells, and co-targeting SREBP-1/FASN/lipogenesis and the AR axis by DFE could be employed as a novel and promising strategy for the treatment of PCa. <i<Davallia formosana</i< anti-prostate cancer efficacy SREBP-1/FASN/lipogenesis AR Neoplasms. Tumors. Oncology. Including cancer and carcinogens Wen-Ping Jiang verfasserin aut Shih-Yin Huang verfasserin aut Praveenkumar Basavaraj verfasserin aut Jin-Bin Wu verfasserin aut Hui-Ya Ho verfasserin aut Guan-Jhong Huang verfasserin aut Wen-Chin Huang verfasserin aut In Cancers MDPI AG, 2010 12(2020), 4, p 914 (DE-627)614095670 (DE-600)2527080-1 20726694 nnns volume:12 year:2020 number:4, p 914 https://doi.org/10.3390/cancers12040914 kostenfrei https://doaj.org/article/deb48556692b4ddd93df265db9e9ae23 kostenfrei https://www.mdpi.com/2072-6694/12/4/914 kostenfrei https://doaj.org/toc/2072-6694 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 4, p 914
allfieldsGer	10.3390/cancers12040914 doi (DE-627)DOAJ078439477 (DE-599)DOAJdeb48556692b4ddd93df265db9e9ae23 DE-627 ger DE-627 rakwb eng RC254-282 Po-Fan Hsieh verfasserin aut Emerging Therapeutic Activity of <i<Davallia formosana</i< on Prostate Cancer Cells through Coordinated Blockade of Lipogenesis and Androgen Receptor Expression 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Prostate cancer (PCa) is the most prevalent malignancy diagnosed in men in Western countries. There is currently no effective therapy for advanced PCa aggressiveness, including castration-resistant progression. The aim of this study is to evaluate the potential efficacy and determine the molecular basis of <i<Davallia formosana</i< (DF) in PCa. Methods: LNCaP (androgen-sensitive) and C4-2 (androgen-insensitive/castration-resistant) PCa cells were utilized in this study. An MTT-based method, a wound healing assay, and the transwell method were performed to evaluate cell proliferation, migration, and invasion. Intracellular fatty acid levels and lipid droplet accumulation were analyzed to determine lipogenesis. Moreover, apoptotic assays and in vivo experiments were conducted. Results: DF ethanol extract (DFE) suppressed proliferation, migration, and invasion in PCa cells. DFE attenuated lipogenesis through inhibition of the expression of sterol regulatory element-binding protein-1 (SREBP-1) and fatty acid synthase (FASN). Moreover, DFE decreased androgen receptor (AR) and prostate-specific antigen (PSA) expression in PCa cells. We further showed the potent therapeutic activity of DFE by repressing the growth and leading to apoptosis of subcutaneous C4-2 tumors in a xenograft mouse model. Conclusions: These data provide a new molecular basis of DFE in PCa cells, and co-targeting SREBP-1/FASN/lipogenesis and the AR axis by DFE could be employed as a novel and promising strategy for the treatment of PCa. <i<Davallia formosana</i< anti-prostate cancer efficacy SREBP-1/FASN/lipogenesis AR Neoplasms. Tumors. Oncology. Including cancer and carcinogens Wen-Ping Jiang verfasserin aut Shih-Yin Huang verfasserin aut Praveenkumar Basavaraj verfasserin aut Jin-Bin Wu verfasserin aut Hui-Ya Ho verfasserin aut Guan-Jhong Huang verfasserin aut Wen-Chin Huang verfasserin aut In Cancers MDPI AG, 2010 12(2020), 4, p 914 (DE-627)614095670 (DE-600)2527080-1 20726694 nnns volume:12 year:2020 number:4, p 914 https://doi.org/10.3390/cancers12040914 kostenfrei https://doaj.org/article/deb48556692b4ddd93df265db9e9ae23 kostenfrei https://www.mdpi.com/2072-6694/12/4/914 kostenfrei https://doaj.org/toc/2072-6694 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 4, p 914
allfieldsSound	10.3390/cancers12040914 doi (DE-627)DOAJ078439477 (DE-599)DOAJdeb48556692b4ddd93df265db9e9ae23 DE-627 ger DE-627 rakwb eng RC254-282 Po-Fan Hsieh verfasserin aut Emerging Therapeutic Activity of <i<Davallia formosana</i< on Prostate Cancer Cells through Coordinated Blockade of Lipogenesis and Androgen Receptor Expression 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Prostate cancer (PCa) is the most prevalent malignancy diagnosed in men in Western countries. There is currently no effective therapy for advanced PCa aggressiveness, including castration-resistant progression. The aim of this study is to evaluate the potential efficacy and determine the molecular basis of <i<Davallia formosana</i< (DF) in PCa. Methods: LNCaP (androgen-sensitive) and C4-2 (androgen-insensitive/castration-resistant) PCa cells were utilized in this study. An MTT-based method, a wound healing assay, and the transwell method were performed to evaluate cell proliferation, migration, and invasion. Intracellular fatty acid levels and lipid droplet accumulation were analyzed to determine lipogenesis. Moreover, apoptotic assays and in vivo experiments were conducted. Results: DF ethanol extract (DFE) suppressed proliferation, migration, and invasion in PCa cells. DFE attenuated lipogenesis through inhibition of the expression of sterol regulatory element-binding protein-1 (SREBP-1) and fatty acid synthase (FASN). Moreover, DFE decreased androgen receptor (AR) and prostate-specific antigen (PSA) expression in PCa cells. We further showed the potent therapeutic activity of DFE by repressing the growth and leading to apoptosis of subcutaneous C4-2 tumors in a xenograft mouse model. Conclusions: These data provide a new molecular basis of DFE in PCa cells, and co-targeting SREBP-1/FASN/lipogenesis and the AR axis by DFE could be employed as a novel and promising strategy for the treatment of PCa. <i<Davallia formosana</i< anti-prostate cancer efficacy SREBP-1/FASN/lipogenesis AR Neoplasms. Tumors. Oncology. Including cancer and carcinogens Wen-Ping Jiang verfasserin aut Shih-Yin Huang verfasserin aut Praveenkumar Basavaraj verfasserin aut Jin-Bin Wu verfasserin aut Hui-Ya Ho verfasserin aut Guan-Jhong Huang verfasserin aut Wen-Chin Huang verfasserin aut In Cancers MDPI AG, 2010 12(2020), 4, p 914 (DE-627)614095670 (DE-600)2527080-1 20726694 nnns volume:12 year:2020 number:4, p 914 https://doi.org/10.3390/cancers12040914 kostenfrei https://doaj.org/article/deb48556692b4ddd93df265db9e9ae23 kostenfrei https://www.mdpi.com/2072-6694/12/4/914 kostenfrei https://doaj.org/toc/2072-6694 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 4, p 914
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authorswithroles_txt_mv	Po-Fan Hsieh @@aut@@ Wen-Ping Jiang @@aut@@ Shih-Yin Huang @@aut@@ Praveenkumar Basavaraj @@aut@@ Jin-Bin Wu @@aut@@ Hui-Ya Ho @@aut@@ Guan-Jhong Huang @@aut@@ Wen-Chin Huang @@aut@@
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callnumber-first	R - Medicine
author	Po-Fan Hsieh
spellingShingle	Po-Fan Hsieh misc RC254-282 misc <i<Davallia formosana</i< misc anti-prostate cancer efficacy misc SREBP-1/FASN/lipogenesis misc AR misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens Emerging Therapeutic Activity of <i<Davallia formosana</i< on Prostate Cancer Cells through Coordinated Blockade of Lipogenesis and Androgen Receptor Expression
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topic_title	RC254-282 Emerging Therapeutic Activity of <i<Davallia formosana</i< on Prostate Cancer Cells through Coordinated Blockade of Lipogenesis and Androgen Receptor Expression <i<Davallia formosana</i< anti-prostate cancer efficacy SREBP-1/FASN/lipogenesis AR
topic	misc RC254-282 misc <i<Davallia formosana</i< misc anti-prostate cancer efficacy misc SREBP-1/FASN/lipogenesis misc AR misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens
topic_unstemmed	misc RC254-282 misc <i<Davallia formosana</i< misc anti-prostate cancer efficacy misc SREBP-1/FASN/lipogenesis misc AR misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens
topic_browse	misc RC254-282 misc <i<Davallia formosana</i< misc anti-prostate cancer efficacy misc SREBP-1/FASN/lipogenesis misc AR misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens
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title	Emerging Therapeutic Activity of <i<Davallia formosana</i< on Prostate Cancer Cells through Coordinated Blockade of Lipogenesis and Androgen Receptor Expression
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title_full	Emerging Therapeutic Activity of <i<Davallia formosana</i< on Prostate Cancer Cells through Coordinated Blockade of Lipogenesis and Androgen Receptor Expression
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title_sort	emerging therapeutic activity of <i<davallia formosana</i< on prostate cancer cells through coordinated blockade of lipogenesis and androgen receptor expression
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title_auth	Emerging Therapeutic Activity of <i<Davallia formosana</i< on Prostate Cancer Cells through Coordinated Blockade of Lipogenesis and Androgen Receptor Expression
abstract	Background: Prostate cancer (PCa) is the most prevalent malignancy diagnosed in men in Western countries. There is currently no effective therapy for advanced PCa aggressiveness, including castration-resistant progression. The aim of this study is to evaluate the potential efficacy and determine the molecular basis of <i<Davallia formosana</i< (DF) in PCa. Methods: LNCaP (androgen-sensitive) and C4-2 (androgen-insensitive/castration-resistant) PCa cells were utilized in this study. An MTT-based method, a wound healing assay, and the transwell method were performed to evaluate cell proliferation, migration, and invasion. Intracellular fatty acid levels and lipid droplet accumulation were analyzed to determine lipogenesis. Moreover, apoptotic assays and in vivo experiments were conducted. Results: DF ethanol extract (DFE) suppressed proliferation, migration, and invasion in PCa cells. DFE attenuated lipogenesis through inhibition of the expression of sterol regulatory element-binding protein-1 (SREBP-1) and fatty acid synthase (FASN). Moreover, DFE decreased androgen receptor (AR) and prostate-specific antigen (PSA) expression in PCa cells. We further showed the potent therapeutic activity of DFE by repressing the growth and leading to apoptosis of subcutaneous C4-2 tumors in a xenograft mouse model. Conclusions: These data provide a new molecular basis of DFE in PCa cells, and co-targeting SREBP-1/FASN/lipogenesis and the AR axis by DFE could be employed as a novel and promising strategy for the treatment of PCa.
abstractGer	Background: Prostate cancer (PCa) is the most prevalent malignancy diagnosed in men in Western countries. There is currently no effective therapy for advanced PCa aggressiveness, including castration-resistant progression. The aim of this study is to evaluate the potential efficacy and determine the molecular basis of <i<Davallia formosana</i< (DF) in PCa. Methods: LNCaP (androgen-sensitive) and C4-2 (androgen-insensitive/castration-resistant) PCa cells were utilized in this study. An MTT-based method, a wound healing assay, and the transwell method were performed to evaluate cell proliferation, migration, and invasion. Intracellular fatty acid levels and lipid droplet accumulation were analyzed to determine lipogenesis. Moreover, apoptotic assays and in vivo experiments were conducted. Results: DF ethanol extract (DFE) suppressed proliferation, migration, and invasion in PCa cells. DFE attenuated lipogenesis through inhibition of the expression of sterol regulatory element-binding protein-1 (SREBP-1) and fatty acid synthase (FASN). Moreover, DFE decreased androgen receptor (AR) and prostate-specific antigen (PSA) expression in PCa cells. We further showed the potent therapeutic activity of DFE by repressing the growth and leading to apoptosis of subcutaneous C4-2 tumors in a xenograft mouse model. Conclusions: These data provide a new molecular basis of DFE in PCa cells, and co-targeting SREBP-1/FASN/lipogenesis and the AR axis by DFE could be employed as a novel and promising strategy for the treatment of PCa.
abstract_unstemmed	Background: Prostate cancer (PCa) is the most prevalent malignancy diagnosed in men in Western countries. There is currently no effective therapy for advanced PCa aggressiveness, including castration-resistant progression. The aim of this study is to evaluate the potential efficacy and determine the molecular basis of <i<Davallia formosana</i< (DF) in PCa. Methods: LNCaP (androgen-sensitive) and C4-2 (androgen-insensitive/castration-resistant) PCa cells were utilized in this study. An MTT-based method, a wound healing assay, and the transwell method were performed to evaluate cell proliferation, migration, and invasion. Intracellular fatty acid levels and lipid droplet accumulation were analyzed to determine lipogenesis. Moreover, apoptotic assays and in vivo experiments were conducted. Results: DF ethanol extract (DFE) suppressed proliferation, migration, and invasion in PCa cells. DFE attenuated lipogenesis through inhibition of the expression of sterol regulatory element-binding protein-1 (SREBP-1) and fatty acid synthase (FASN). Moreover, DFE decreased androgen receptor (AR) and prostate-specific antigen (PSA) expression in PCa cells. We further showed the potent therapeutic activity of DFE by repressing the growth and leading to apoptosis of subcutaneous C4-2 tumors in a xenograft mouse model. Conclusions: These data provide a new molecular basis of DFE in PCa cells, and co-targeting SREBP-1/FASN/lipogenesis and the AR axis by DFE could be employed as a novel and promising strategy for the treatment of PCa.
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title_short	Emerging Therapeutic Activity of <i<Davallia formosana</i< on Prostate Cancer Cells through Coordinated Blockade of Lipogenesis and Androgen Receptor Expression
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There is currently no effective therapy for advanced PCa aggressiveness, including castration-resistant progression. The aim of this study is to evaluate the potential efficacy and determine the molecular basis of <i<Davallia formosana</i< (DF) in PCa. Methods: LNCaP (androgen-sensitive) and C4-2 (androgen-insensitive/castration-resistant) PCa cells were utilized in this study. An MTT-based method, a wound healing assay, and the transwell method were performed to evaluate cell proliferation, migration, and invasion. Intracellular fatty acid levels and lipid droplet accumulation were analyzed to determine lipogenesis. Moreover, apoptotic assays and in vivo experiments were conducted. Results: DF ethanol extract (DFE) suppressed proliferation, migration, and invasion in PCa cells. DFE attenuated lipogenesis through inhibition of the expression of sterol regulatory element-binding protein-1 (SREBP-1) and fatty acid synthase (FASN). Moreover, DFE decreased androgen receptor (AR) and prostate-specific antigen (PSA) expression in PCa cells. We further showed the potent therapeutic activity of DFE by repressing the growth and leading to apoptosis of subcutaneous C4-2 tumors in a xenograft mouse model. Conclusions: These data provide a new molecular basis of DFE in PCa cells, and co-targeting SREBP-1/FASN/lipogenesis and the AR axis by DFE could be employed as a novel and promising strategy for the treatment of PCa.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a"><i<Davallia formosana</i<</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">anti-prostate cancer efficacy</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">SREBP-1/FASN/lipogenesis</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">AR</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Neoplasms. Tumors. 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Emerging Therapeutic Activity of <i<Davallia formosana</i< on Prostate Cancer Cells through Coordinated Blockade of Lipogenesis and Androgen Receptor Expression

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