Reduced intraepidermal nerve fibre density, glial activation, and sensory changes in HIV type-1 Tat-expressing female mice: involvement of Tat during early stages of HIV-associated painful sensory neuropathy
Abstract. Introduction:. HIV infection is associated with chronic pain states, including sensory neuropathy, which affects greater than 40% of patients. Objectives and Methods:. To determine the impact of HIV-Tat induction on nociceptive behaviour in female mice conditionally expressing HIV Tat1-86...
Ausführliche Beschreibung
Autor*in: |
Rachel Wodarski [verfasserIn] Deniz Bagdas [verfasserIn] Jason J. Paris [verfasserIn] Tim Pheby [verfasserIn] Wisam Toma [verfasserIn] Ruqiang Xu [verfasserIn] M. Imad Damaj [verfasserIn] Pamela E. Knapp [verfasserIn] Andrew S.C. Rice [verfasserIn] Kurt F. Hauser [verfasserIn] |
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E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2018 |
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Übergeordnetes Werk: |
In: PAIN Reports - Wolters Kluwer, 2017, 3(2018), 3, p e654 |
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Übergeordnetes Werk: |
volume:3 ; year:2018 ; number:3, p e654 |
Links: |
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DOI / URN: |
10.1097/PR9.0000000000000654 |
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Katalog-ID: |
DOAJ07854193X |
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520 | |a Abstract. Introduction:. HIV infection is associated with chronic pain states, including sensory neuropathy, which affects greater than 40% of patients. Objectives and Methods:. To determine the impact of HIV-Tat induction on nociceptive behaviour in female mice conditionally expressing HIV Tat1-86 protein through a doxycycline (DOX)-driven glial fibrillary acidic protein promoter, intraepidermal nerve fibre density and immune cell activation in the dorsal root ganglion (DRG) and spinal cord were assessed by immunohistochemistry. Mice were assessed for mechanical and thermal sensitivity for 9 weeks using von-Frey and Hargreaves tests. Results:. Intraepidermal nerve fibre density was significantly reduced after 6 weeks of Tat induction, similar to sensory neuropathy seen in clinical HIV infection. Tat induction through DOX caused a significant reduction in paw withdrawal thresholds in a time-dependent manner starting the 4th week after Tat induction. No changes in paw withdrawal latencies were seen in Tat(−) control mice lacking the tat transgene. Although reductions in paw withdrawal thresholds increased throughout the study, no significant change in spontaneous motor activity was observed. Spinal cord (cervical and lumbar), DRG, and hind paw skin were collected at 8 days and 6 weeks after Tat induction. HIV-Tat mRNA expression was significantly increased in lumbar DRG and skin samples 8 days after DOX treatment. Tat induced a significant increase in the number of Iba-1 positive cells at 6 weeks, but not after 8 days, of exposure. No differences in glial fibrillary acidic protein immunoreactivity were observed. Conclusion:. These results suggest that Tat protein contributes to painful HIV-related sensory neuropathy during the initial stages of the pathogenesis. | ||
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700 | 0 | |a Wisam Toma |e verfasserin |4 aut | |
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700 | 0 | |a Andrew S.C. Rice |e verfasserin |4 aut | |
700 | 0 | |a Kurt F. Hauser |e verfasserin |4 aut | |
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10.1097/PR9.0000000000000654 doi (DE-627)DOAJ07854193X (DE-599)DOAJ59c0ff0d238a4c6382ece5fd44aabbb5 DE-627 ger DE-627 rakwb eng RD78.3-87.3 Rachel Wodarski verfasserin aut Reduced intraepidermal nerve fibre density, glial activation, and sensory changes in HIV type-1 Tat-expressing female mice: involvement of Tat during early stages of HIV-associated painful sensory neuropathy 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract. Introduction:. HIV infection is associated with chronic pain states, including sensory neuropathy, which affects greater than 40% of patients. Objectives and Methods:. To determine the impact of HIV-Tat induction on nociceptive behaviour in female mice conditionally expressing HIV Tat1-86 protein through a doxycycline (DOX)-driven glial fibrillary acidic protein promoter, intraepidermal nerve fibre density and immune cell activation in the dorsal root ganglion (DRG) and spinal cord were assessed by immunohistochemistry. Mice were assessed for mechanical and thermal sensitivity for 9 weeks using von-Frey and Hargreaves tests. Results:. Intraepidermal nerve fibre density was significantly reduced after 6 weeks of Tat induction, similar to sensory neuropathy seen in clinical HIV infection. Tat induction through DOX caused a significant reduction in paw withdrawal thresholds in a time-dependent manner starting the 4th week after Tat induction. No changes in paw withdrawal latencies were seen in Tat(−) control mice lacking the tat transgene. Although reductions in paw withdrawal thresholds increased throughout the study, no significant change in spontaneous motor activity was observed. Spinal cord (cervical and lumbar), DRG, and hind paw skin were collected at 8 days and 6 weeks after Tat induction. HIV-Tat mRNA expression was significantly increased in lumbar DRG and skin samples 8 days after DOX treatment. Tat induced a significant increase in the number of Iba-1 positive cells at 6 weeks, but not after 8 days, of exposure. No differences in glial fibrillary acidic protein immunoreactivity were observed. Conclusion:. These results suggest that Tat protein contributes to painful HIV-related sensory neuropathy during the initial stages of the pathogenesis. Anesthesiology Deniz Bagdas verfasserin aut Jason J. Paris verfasserin aut Tim Pheby verfasserin aut Wisam Toma verfasserin aut Ruqiang Xu verfasserin aut M. Imad Damaj verfasserin aut Pamela E. Knapp verfasserin aut Andrew S.C. Rice verfasserin aut Kurt F. Hauser verfasserin aut In PAIN Reports Wolters Kluwer, 2017 3(2018), 3, p e654 (DE-627)891074198 (DE-600)2898347-6 24712531 nnns volume:3 year:2018 number:3, p e654 https://doi.org/10.1097/PR9.0000000000000654 kostenfrei https://doaj.org/article/59c0ff0d238a4c6382ece5fd44aabbb5 kostenfrei http://journals.lww.com/painrpts/fulltext/10.1097/PR9.0000000000000654 kostenfrei https://doaj.org/toc/2471-2531 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2018 3, p e654 |
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10.1097/PR9.0000000000000654 doi (DE-627)DOAJ07854193X (DE-599)DOAJ59c0ff0d238a4c6382ece5fd44aabbb5 DE-627 ger DE-627 rakwb eng RD78.3-87.3 Rachel Wodarski verfasserin aut Reduced intraepidermal nerve fibre density, glial activation, and sensory changes in HIV type-1 Tat-expressing female mice: involvement of Tat during early stages of HIV-associated painful sensory neuropathy 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract. Introduction:. HIV infection is associated with chronic pain states, including sensory neuropathy, which affects greater than 40% of patients. Objectives and Methods:. To determine the impact of HIV-Tat induction on nociceptive behaviour in female mice conditionally expressing HIV Tat1-86 protein through a doxycycline (DOX)-driven glial fibrillary acidic protein promoter, intraepidermal nerve fibre density and immune cell activation in the dorsal root ganglion (DRG) and spinal cord were assessed by immunohistochemistry. Mice were assessed for mechanical and thermal sensitivity for 9 weeks using von-Frey and Hargreaves tests. Results:. Intraepidermal nerve fibre density was significantly reduced after 6 weeks of Tat induction, similar to sensory neuropathy seen in clinical HIV infection. Tat induction through DOX caused a significant reduction in paw withdrawal thresholds in a time-dependent manner starting the 4th week after Tat induction. No changes in paw withdrawal latencies were seen in Tat(−) control mice lacking the tat transgene. Although reductions in paw withdrawal thresholds increased throughout the study, no significant change in spontaneous motor activity was observed. Spinal cord (cervical and lumbar), DRG, and hind paw skin were collected at 8 days and 6 weeks after Tat induction. HIV-Tat mRNA expression was significantly increased in lumbar DRG and skin samples 8 days after DOX treatment. Tat induced a significant increase in the number of Iba-1 positive cells at 6 weeks, but not after 8 days, of exposure. No differences in glial fibrillary acidic protein immunoreactivity were observed. Conclusion:. These results suggest that Tat protein contributes to painful HIV-related sensory neuropathy during the initial stages of the pathogenesis. Anesthesiology Deniz Bagdas verfasserin aut Jason J. Paris verfasserin aut Tim Pheby verfasserin aut Wisam Toma verfasserin aut Ruqiang Xu verfasserin aut M. Imad Damaj verfasserin aut Pamela E. Knapp verfasserin aut Andrew S.C. Rice verfasserin aut Kurt F. Hauser verfasserin aut In PAIN Reports Wolters Kluwer, 2017 3(2018), 3, p e654 (DE-627)891074198 (DE-600)2898347-6 24712531 nnns volume:3 year:2018 number:3, p e654 https://doi.org/10.1097/PR9.0000000000000654 kostenfrei https://doaj.org/article/59c0ff0d238a4c6382ece5fd44aabbb5 kostenfrei http://journals.lww.com/painrpts/fulltext/10.1097/PR9.0000000000000654 kostenfrei https://doaj.org/toc/2471-2531 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2018 3, p e654 |
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10.1097/PR9.0000000000000654 doi (DE-627)DOAJ07854193X (DE-599)DOAJ59c0ff0d238a4c6382ece5fd44aabbb5 DE-627 ger DE-627 rakwb eng RD78.3-87.3 Rachel Wodarski verfasserin aut Reduced intraepidermal nerve fibre density, glial activation, and sensory changes in HIV type-1 Tat-expressing female mice: involvement of Tat during early stages of HIV-associated painful sensory neuropathy 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract. Introduction:. HIV infection is associated with chronic pain states, including sensory neuropathy, which affects greater than 40% of patients. Objectives and Methods:. To determine the impact of HIV-Tat induction on nociceptive behaviour in female mice conditionally expressing HIV Tat1-86 protein through a doxycycline (DOX)-driven glial fibrillary acidic protein promoter, intraepidermal nerve fibre density and immune cell activation in the dorsal root ganglion (DRG) and spinal cord were assessed by immunohistochemistry. Mice were assessed for mechanical and thermal sensitivity for 9 weeks using von-Frey and Hargreaves tests. Results:. Intraepidermal nerve fibre density was significantly reduced after 6 weeks of Tat induction, similar to sensory neuropathy seen in clinical HIV infection. Tat induction through DOX caused a significant reduction in paw withdrawal thresholds in a time-dependent manner starting the 4th week after Tat induction. No changes in paw withdrawal latencies were seen in Tat(−) control mice lacking the tat transgene. Although reductions in paw withdrawal thresholds increased throughout the study, no significant change in spontaneous motor activity was observed. Spinal cord (cervical and lumbar), DRG, and hind paw skin were collected at 8 days and 6 weeks after Tat induction. HIV-Tat mRNA expression was significantly increased in lumbar DRG and skin samples 8 days after DOX treatment. Tat induced a significant increase in the number of Iba-1 positive cells at 6 weeks, but not after 8 days, of exposure. No differences in glial fibrillary acidic protein immunoreactivity were observed. Conclusion:. These results suggest that Tat protein contributes to painful HIV-related sensory neuropathy during the initial stages of the pathogenesis. Anesthesiology Deniz Bagdas verfasserin aut Jason J. Paris verfasserin aut Tim Pheby verfasserin aut Wisam Toma verfasserin aut Ruqiang Xu verfasserin aut M. Imad Damaj verfasserin aut Pamela E. Knapp verfasserin aut Andrew S.C. Rice verfasserin aut Kurt F. Hauser verfasserin aut In PAIN Reports Wolters Kluwer, 2017 3(2018), 3, p e654 (DE-627)891074198 (DE-600)2898347-6 24712531 nnns volume:3 year:2018 number:3, p e654 https://doi.org/10.1097/PR9.0000000000000654 kostenfrei https://doaj.org/article/59c0ff0d238a4c6382ece5fd44aabbb5 kostenfrei http://journals.lww.com/painrpts/fulltext/10.1097/PR9.0000000000000654 kostenfrei https://doaj.org/toc/2471-2531 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2018 3, p e654 |
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10.1097/PR9.0000000000000654 doi (DE-627)DOAJ07854193X (DE-599)DOAJ59c0ff0d238a4c6382ece5fd44aabbb5 DE-627 ger DE-627 rakwb eng RD78.3-87.3 Rachel Wodarski verfasserin aut Reduced intraepidermal nerve fibre density, glial activation, and sensory changes in HIV type-1 Tat-expressing female mice: involvement of Tat during early stages of HIV-associated painful sensory neuropathy 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract. Introduction:. HIV infection is associated with chronic pain states, including sensory neuropathy, which affects greater than 40% of patients. Objectives and Methods:. To determine the impact of HIV-Tat induction on nociceptive behaviour in female mice conditionally expressing HIV Tat1-86 protein through a doxycycline (DOX)-driven glial fibrillary acidic protein promoter, intraepidermal nerve fibre density and immune cell activation in the dorsal root ganglion (DRG) and spinal cord were assessed by immunohistochemistry. Mice were assessed for mechanical and thermal sensitivity for 9 weeks using von-Frey and Hargreaves tests. Results:. Intraepidermal nerve fibre density was significantly reduced after 6 weeks of Tat induction, similar to sensory neuropathy seen in clinical HIV infection. Tat induction through DOX caused a significant reduction in paw withdrawal thresholds in a time-dependent manner starting the 4th week after Tat induction. No changes in paw withdrawal latencies were seen in Tat(−) control mice lacking the tat transgene. Although reductions in paw withdrawal thresholds increased throughout the study, no significant change in spontaneous motor activity was observed. Spinal cord (cervical and lumbar), DRG, and hind paw skin were collected at 8 days and 6 weeks after Tat induction. HIV-Tat mRNA expression was significantly increased in lumbar DRG and skin samples 8 days after DOX treatment. Tat induced a significant increase in the number of Iba-1 positive cells at 6 weeks, but not after 8 days, of exposure. No differences in glial fibrillary acidic protein immunoreactivity were observed. Conclusion:. These results suggest that Tat protein contributes to painful HIV-related sensory neuropathy during the initial stages of the pathogenesis. Anesthesiology Deniz Bagdas verfasserin aut Jason J. Paris verfasserin aut Tim Pheby verfasserin aut Wisam Toma verfasserin aut Ruqiang Xu verfasserin aut M. Imad Damaj verfasserin aut Pamela E. Knapp verfasserin aut Andrew S.C. Rice verfasserin aut Kurt F. Hauser verfasserin aut In PAIN Reports Wolters Kluwer, 2017 3(2018), 3, p e654 (DE-627)891074198 (DE-600)2898347-6 24712531 nnns volume:3 year:2018 number:3, p e654 https://doi.org/10.1097/PR9.0000000000000654 kostenfrei https://doaj.org/article/59c0ff0d238a4c6382ece5fd44aabbb5 kostenfrei http://journals.lww.com/painrpts/fulltext/10.1097/PR9.0000000000000654 kostenfrei https://doaj.org/toc/2471-2531 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2018 3, p e654 |
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10.1097/PR9.0000000000000654 doi (DE-627)DOAJ07854193X (DE-599)DOAJ59c0ff0d238a4c6382ece5fd44aabbb5 DE-627 ger DE-627 rakwb eng RD78.3-87.3 Rachel Wodarski verfasserin aut Reduced intraepidermal nerve fibre density, glial activation, and sensory changes in HIV type-1 Tat-expressing female mice: involvement of Tat during early stages of HIV-associated painful sensory neuropathy 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract. Introduction:. HIV infection is associated with chronic pain states, including sensory neuropathy, which affects greater than 40% of patients. Objectives and Methods:. To determine the impact of HIV-Tat induction on nociceptive behaviour in female mice conditionally expressing HIV Tat1-86 protein through a doxycycline (DOX)-driven glial fibrillary acidic protein promoter, intraepidermal nerve fibre density and immune cell activation in the dorsal root ganglion (DRG) and spinal cord were assessed by immunohistochemistry. Mice were assessed for mechanical and thermal sensitivity for 9 weeks using von-Frey and Hargreaves tests. Results:. Intraepidermal nerve fibre density was significantly reduced after 6 weeks of Tat induction, similar to sensory neuropathy seen in clinical HIV infection. Tat induction through DOX caused a significant reduction in paw withdrawal thresholds in a time-dependent manner starting the 4th week after Tat induction. No changes in paw withdrawal latencies were seen in Tat(−) control mice lacking the tat transgene. Although reductions in paw withdrawal thresholds increased throughout the study, no significant change in spontaneous motor activity was observed. Spinal cord (cervical and lumbar), DRG, and hind paw skin were collected at 8 days and 6 weeks after Tat induction. HIV-Tat mRNA expression was significantly increased in lumbar DRG and skin samples 8 days after DOX treatment. Tat induced a significant increase in the number of Iba-1 positive cells at 6 weeks, but not after 8 days, of exposure. No differences in glial fibrillary acidic protein immunoreactivity were observed. Conclusion:. These results suggest that Tat protein contributes to painful HIV-related sensory neuropathy during the initial stages of the pathogenesis. Anesthesiology Deniz Bagdas verfasserin aut Jason J. Paris verfasserin aut Tim Pheby verfasserin aut Wisam Toma verfasserin aut Ruqiang Xu verfasserin aut M. Imad Damaj verfasserin aut Pamela E. Knapp verfasserin aut Andrew S.C. Rice verfasserin aut Kurt F. Hauser verfasserin aut In PAIN Reports Wolters Kluwer, 2017 3(2018), 3, p e654 (DE-627)891074198 (DE-600)2898347-6 24712531 nnns volume:3 year:2018 number:3, p e654 https://doi.org/10.1097/PR9.0000000000000654 kostenfrei https://doaj.org/article/59c0ff0d238a4c6382ece5fd44aabbb5 kostenfrei http://journals.lww.com/painrpts/fulltext/10.1097/PR9.0000000000000654 kostenfrei https://doaj.org/toc/2471-2531 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2018 3, p e654 |
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Reduced intraepidermal nerve fibre density, glial activation, and sensory changes in HIV type-1 Tat-expressing female mice: involvement of Tat during early stages of HIV-associated painful sensory neuropathy |
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reduced intraepidermal nerve fibre density, glial activation, and sensory changes in hiv type-1 tat-expressing female mice: involvement of tat during early stages of hiv-associated painful sensory neuropathy |
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Reduced intraepidermal nerve fibre density, glial activation, and sensory changes in HIV type-1 Tat-expressing female mice: involvement of Tat during early stages of HIV-associated painful sensory neuropathy |
abstract |
Abstract. Introduction:. HIV infection is associated with chronic pain states, including sensory neuropathy, which affects greater than 40% of patients. Objectives and Methods:. To determine the impact of HIV-Tat induction on nociceptive behaviour in female mice conditionally expressing HIV Tat1-86 protein through a doxycycline (DOX)-driven glial fibrillary acidic protein promoter, intraepidermal nerve fibre density and immune cell activation in the dorsal root ganglion (DRG) and spinal cord were assessed by immunohistochemistry. Mice were assessed for mechanical and thermal sensitivity for 9 weeks using von-Frey and Hargreaves tests. Results:. Intraepidermal nerve fibre density was significantly reduced after 6 weeks of Tat induction, similar to sensory neuropathy seen in clinical HIV infection. Tat induction through DOX caused a significant reduction in paw withdrawal thresholds in a time-dependent manner starting the 4th week after Tat induction. No changes in paw withdrawal latencies were seen in Tat(−) control mice lacking the tat transgene. Although reductions in paw withdrawal thresholds increased throughout the study, no significant change in spontaneous motor activity was observed. Spinal cord (cervical and lumbar), DRG, and hind paw skin were collected at 8 days and 6 weeks after Tat induction. HIV-Tat mRNA expression was significantly increased in lumbar DRG and skin samples 8 days after DOX treatment. Tat induced a significant increase in the number of Iba-1 positive cells at 6 weeks, but not after 8 days, of exposure. No differences in glial fibrillary acidic protein immunoreactivity were observed. Conclusion:. These results suggest that Tat protein contributes to painful HIV-related sensory neuropathy during the initial stages of the pathogenesis. |
abstractGer |
Abstract. Introduction:. HIV infection is associated with chronic pain states, including sensory neuropathy, which affects greater than 40% of patients. Objectives and Methods:. To determine the impact of HIV-Tat induction on nociceptive behaviour in female mice conditionally expressing HIV Tat1-86 protein through a doxycycline (DOX)-driven glial fibrillary acidic protein promoter, intraepidermal nerve fibre density and immune cell activation in the dorsal root ganglion (DRG) and spinal cord were assessed by immunohistochemistry. Mice were assessed for mechanical and thermal sensitivity for 9 weeks using von-Frey and Hargreaves tests. Results:. Intraepidermal nerve fibre density was significantly reduced after 6 weeks of Tat induction, similar to sensory neuropathy seen in clinical HIV infection. Tat induction through DOX caused a significant reduction in paw withdrawal thresholds in a time-dependent manner starting the 4th week after Tat induction. No changes in paw withdrawal latencies were seen in Tat(−) control mice lacking the tat transgene. Although reductions in paw withdrawal thresholds increased throughout the study, no significant change in spontaneous motor activity was observed. Spinal cord (cervical and lumbar), DRG, and hind paw skin were collected at 8 days and 6 weeks after Tat induction. HIV-Tat mRNA expression was significantly increased in lumbar DRG and skin samples 8 days after DOX treatment. Tat induced a significant increase in the number of Iba-1 positive cells at 6 weeks, but not after 8 days, of exposure. No differences in glial fibrillary acidic protein immunoreactivity were observed. Conclusion:. These results suggest that Tat protein contributes to painful HIV-related sensory neuropathy during the initial stages of the pathogenesis. |
abstract_unstemmed |
Abstract. Introduction:. HIV infection is associated with chronic pain states, including sensory neuropathy, which affects greater than 40% of patients. Objectives and Methods:. To determine the impact of HIV-Tat induction on nociceptive behaviour in female mice conditionally expressing HIV Tat1-86 protein through a doxycycline (DOX)-driven glial fibrillary acidic protein promoter, intraepidermal nerve fibre density and immune cell activation in the dorsal root ganglion (DRG) and spinal cord were assessed by immunohistochemistry. Mice were assessed for mechanical and thermal sensitivity for 9 weeks using von-Frey and Hargreaves tests. Results:. Intraepidermal nerve fibre density was significantly reduced after 6 weeks of Tat induction, similar to sensory neuropathy seen in clinical HIV infection. Tat induction through DOX caused a significant reduction in paw withdrawal thresholds in a time-dependent manner starting the 4th week after Tat induction. No changes in paw withdrawal latencies were seen in Tat(−) control mice lacking the tat transgene. Although reductions in paw withdrawal thresholds increased throughout the study, no significant change in spontaneous motor activity was observed. Spinal cord (cervical and lumbar), DRG, and hind paw skin were collected at 8 days and 6 weeks after Tat induction. HIV-Tat mRNA expression was significantly increased in lumbar DRG and skin samples 8 days after DOX treatment. Tat induced a significant increase in the number of Iba-1 positive cells at 6 weeks, but not after 8 days, of exposure. No differences in glial fibrillary acidic protein immunoreactivity were observed. Conclusion:. These results suggest that Tat protein contributes to painful HIV-related sensory neuropathy during the initial stages of the pathogenesis. |
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Reduced intraepidermal nerve fibre density, glial activation, and sensory changes in HIV type-1 Tat-expressing female mice: involvement of Tat during early stages of HIV-associated painful sensory neuropathy |
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https://doi.org/10.1097/PR9.0000000000000654 https://doaj.org/article/59c0ff0d238a4c6382ece5fd44aabbb5 http://journals.lww.com/painrpts/fulltext/10.1097/PR9.0000000000000654 https://doaj.org/toc/2471-2531 |
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Introduction:. HIV infection is associated with chronic pain states, including sensory neuropathy, which affects greater than 40% of patients. Objectives and Methods:. To determine the impact of HIV-Tat induction on nociceptive behaviour in female mice conditionally expressing HIV Tat1-86 protein through a doxycycline (DOX)-driven glial fibrillary acidic protein promoter, intraepidermal nerve fibre density and immune cell activation in the dorsal root ganglion (DRG) and spinal cord were assessed by immunohistochemistry. Mice were assessed for mechanical and thermal sensitivity for 9 weeks using von-Frey and Hargreaves tests. Results:. Intraepidermal nerve fibre density was significantly reduced after 6 weeks of Tat induction, similar to sensory neuropathy seen in clinical HIV infection. Tat induction through DOX caused a significant reduction in paw withdrawal thresholds in a time-dependent manner starting the 4th week after Tat induction. No changes in paw withdrawal latencies were seen in Tat(−) control mice lacking the tat transgene. Although reductions in paw withdrawal thresholds increased throughout the study, no significant change in spontaneous motor activity was observed. Spinal cord (cervical and lumbar), DRG, and hind paw skin were collected at 8 days and 6 weeks after Tat induction. HIV-Tat mRNA expression was significantly increased in lumbar DRG and skin samples 8 days after DOX treatment. Tat induced a significant increase in the number of Iba-1 positive cells at 6 weeks, but not after 8 days, of exposure. No differences in glial fibrillary acidic protein immunoreactivity were observed. Conclusion:. These results suggest that Tat protein contributes to painful HIV-related sensory neuropathy during the initial stages of the pathogenesis.</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Anesthesiology</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Deniz Bagdas</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Jason J. 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