Monocyte Chemotactic Protein-1 (MCP1) Accumulation in Human Osteoclast Precursor Cultures

In vitro osteoclast methods require constant treatment with macrophage colony stimulating factor (M-CSF) to support precursor survival and addition of the differentiation agent receptor activator of NF-κB ligand (RANKL). Constant exposure to granulocyte macrophage colony stimulating factor (GM-CSF)...
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Gespeichert in:

Autor*in:	Nigel A. Morrison [verfasserIn] Mark R. Forwood [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	monocyte chemotactic protein 1 (MCP-1) osteoclast macrophage colony stimulating factor (M-CSF) granulocyte macrophage colony stimulating factor (GM-CSF) receptor activator of NF-κB ligand (RANKL) human CD14+ mononuclear cells

Übergeordnetes Werk:	In: Life - MDPI AG, 2012, 12(2022), 6, p 789
Übergeordnetes Werk:	volume:12 ; year:2022 ; number:6, p 789

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/life12060789

Katalog-ID:	DOAJ078970687

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spelling	10.3390/life12060789 doi (DE-627)DOAJ078970687 (DE-599)DOAJ2901c2bc7580443b8de37aae19daffc6 DE-627 ger DE-627 rakwb eng Nigel A. Morrison verfasserin aut Monocyte Chemotactic Protein-1 (MCP1) Accumulation in Human Osteoclast Precursor Cultures 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In vitro osteoclast methods require constant treatment with macrophage colony stimulating factor (M-CSF) to support precursor survival and addition of the differentiation agent receptor activator of NF-κB ligand (RANKL). Constant exposure to granulocyte macrophage colony stimulating factor (GM-CSF) suppresses human osteoclast formation in vitro. Addition of the chemokine monocyte chemotactic protein-1 (MCP1) to such cultures dramatically increases osteoclast formation and overcomes GM-CSF mediated suppression. We investigated the effect of M-CSF, GM-CSF and the combination of M-CSF and GM-CSF treatment on the expression of chemokines in human CD14+ cells in culture. Of assayed chemokines, MCP1 was the most abundant in terms of mRNA transcript and protein in M-CSF treated cultures and was suppressed by GM-CSF. MCP1 protein accumulated up to 50 ng/mL in culture medium, greatly exceeding other assayed chemokines. C-C chemokine receptor-2 (CCR2) is the receptor for MCP1: the formation of osteoclast-like cells was inhibited by constant exposure to the CCR2 antagonist RS102895, in part by decreasing expression of RANK, the receptor for RANKL. monocyte chemotactic protein 1 (MCP-1) osteoclast macrophage colony stimulating factor (M-CSF) granulocyte macrophage colony stimulating factor (GM-CSF) receptor activator of NF-κB ligand (RANKL) human CD14+ mononuclear cells Science Q Mark R. Forwood verfasserin aut In Life MDPI AG, 2012 12(2022), 6, p 789 (DE-627)718627156 (DE-600)2662250-6 20751729 nnns volume:12 year:2022 number:6, p 789 https://doi.org/10.3390/life12060789 kostenfrei https://doaj.org/article/2901c2bc7580443b8de37aae19daffc6 kostenfrei https://www.mdpi.com/2075-1729/12/6/789 kostenfrei https://doaj.org/toc/2075-1729 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 6, p 789
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allfieldsSound	10.3390/life12060789 doi (DE-627)DOAJ078970687 (DE-599)DOAJ2901c2bc7580443b8de37aae19daffc6 DE-627 ger DE-627 rakwb eng Nigel A. Morrison verfasserin aut Monocyte Chemotactic Protein-1 (MCP1) Accumulation in Human Osteoclast Precursor Cultures 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In vitro osteoclast methods require constant treatment with macrophage colony stimulating factor (M-CSF) to support precursor survival and addition of the differentiation agent receptor activator of NF-κB ligand (RANKL). Constant exposure to granulocyte macrophage colony stimulating factor (GM-CSF) suppresses human osteoclast formation in vitro. Addition of the chemokine monocyte chemotactic protein-1 (MCP1) to such cultures dramatically increases osteoclast formation and overcomes GM-CSF mediated suppression. We investigated the effect of M-CSF, GM-CSF and the combination of M-CSF and GM-CSF treatment on the expression of chemokines in human CD14+ cells in culture. Of assayed chemokines, MCP1 was the most abundant in terms of mRNA transcript and protein in M-CSF treated cultures and was suppressed by GM-CSF. MCP1 protein accumulated up to 50 ng/mL in culture medium, greatly exceeding other assayed chemokines. C-C chemokine receptor-2 (CCR2) is the receptor for MCP1: the formation of osteoclast-like cells was inhibited by constant exposure to the CCR2 antagonist RS102895, in part by decreasing expression of RANK, the receptor for RANKL. monocyte chemotactic protein 1 (MCP-1) osteoclast macrophage colony stimulating factor (M-CSF) granulocyte macrophage colony stimulating factor (GM-CSF) receptor activator of NF-κB ligand (RANKL) human CD14+ mononuclear cells Science Q Mark R. Forwood verfasserin aut In Life MDPI AG, 2012 12(2022), 6, p 789 (DE-627)718627156 (DE-600)2662250-6 20751729 nnns volume:12 year:2022 number:6, p 789 https://doi.org/10.3390/life12060789 kostenfrei https://doaj.org/article/2901c2bc7580443b8de37aae19daffc6 kostenfrei https://www.mdpi.com/2075-1729/12/6/789 kostenfrei https://doaj.org/toc/2075-1729 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 6, p 789
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topic_facet	monocyte chemotactic protein 1 (MCP-1) osteoclast macrophage colony stimulating factor (M-CSF) granulocyte macrophage colony stimulating factor (GM-CSF) receptor activator of NF-κB ligand (RANKL) human CD14+ mononuclear cells Science Q
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author	Nigel A. Morrison
spellingShingle	Nigel A. Morrison misc monocyte chemotactic protein 1 (MCP-1) misc osteoclast misc macrophage colony stimulating factor (M-CSF) misc granulocyte macrophage colony stimulating factor (GM-CSF) misc receptor activator of NF-κB ligand (RANKL) misc human CD14+ mononuclear cells misc Science misc Q Monocyte Chemotactic Protein-1 (MCP1) Accumulation in Human Osteoclast Precursor Cultures
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topic_title	Monocyte Chemotactic Protein-1 (MCP1) Accumulation in Human Osteoclast Precursor Cultures monocyte chemotactic protein 1 (MCP-1) osteoclast macrophage colony stimulating factor (M-CSF) granulocyte macrophage colony stimulating factor (GM-CSF) receptor activator of NF-κB ligand (RANKL) human CD14+ mononuclear cells
topic	misc monocyte chemotactic protein 1 (MCP-1) misc osteoclast misc macrophage colony stimulating factor (M-CSF) misc granulocyte macrophage colony stimulating factor (GM-CSF) misc receptor activator of NF-κB ligand (RANKL) misc human CD14+ mononuclear cells misc Science misc Q
topic_unstemmed	misc monocyte chemotactic protein 1 (MCP-1) misc osteoclast misc macrophage colony stimulating factor (M-CSF) misc granulocyte macrophage colony stimulating factor (GM-CSF) misc receptor activator of NF-κB ligand (RANKL) misc human CD14+ mononuclear cells misc Science misc Q
topic_browse	misc monocyte chemotactic protein 1 (MCP-1) misc osteoclast misc macrophage colony stimulating factor (M-CSF) misc granulocyte macrophage colony stimulating factor (GM-CSF) misc receptor activator of NF-κB ligand (RANKL) misc human CD14+ mononuclear cells misc Science misc Q
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title	Monocyte Chemotactic Protein-1 (MCP1) Accumulation in Human Osteoclast Precursor Cultures
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title_full	Monocyte Chemotactic Protein-1 (MCP1) Accumulation in Human Osteoclast Precursor Cultures
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author_browse	Nigel A. Morrison Mark R. Forwood
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title_sort	monocyte chemotactic protein-1 (mcp1) accumulation in human osteoclast precursor cultures
title_auth	Monocyte Chemotactic Protein-1 (MCP1) Accumulation in Human Osteoclast Precursor Cultures
abstract	In vitro osteoclast methods require constant treatment with macrophage colony stimulating factor (M-CSF) to support precursor survival and addition of the differentiation agent receptor activator of NF-κB ligand (RANKL). Constant exposure to granulocyte macrophage colony stimulating factor (GM-CSF) suppresses human osteoclast formation in vitro. Addition of the chemokine monocyte chemotactic protein-1 (MCP1) to such cultures dramatically increases osteoclast formation and overcomes GM-CSF mediated suppression. We investigated the effect of M-CSF, GM-CSF and the combination of M-CSF and GM-CSF treatment on the expression of chemokines in human CD14+ cells in culture. Of assayed chemokines, MCP1 was the most abundant in terms of mRNA transcript and protein in M-CSF treated cultures and was suppressed by GM-CSF. MCP1 protein accumulated up to 50 ng/mL in culture medium, greatly exceeding other assayed chemokines. C-C chemokine receptor-2 (CCR2) is the receptor for MCP1: the formation of osteoclast-like cells was inhibited by constant exposure to the CCR2 antagonist RS102895, in part by decreasing expression of RANK, the receptor for RANKL.
abstractGer	In vitro osteoclast methods require constant treatment with macrophage colony stimulating factor (M-CSF) to support precursor survival and addition of the differentiation agent receptor activator of NF-κB ligand (RANKL). Constant exposure to granulocyte macrophage colony stimulating factor (GM-CSF) suppresses human osteoclast formation in vitro. Addition of the chemokine monocyte chemotactic protein-1 (MCP1) to such cultures dramatically increases osteoclast formation and overcomes GM-CSF mediated suppression. We investigated the effect of M-CSF, GM-CSF and the combination of M-CSF and GM-CSF treatment on the expression of chemokines in human CD14+ cells in culture. Of assayed chemokines, MCP1 was the most abundant in terms of mRNA transcript and protein in M-CSF treated cultures and was suppressed by GM-CSF. MCP1 protein accumulated up to 50 ng/mL in culture medium, greatly exceeding other assayed chemokines. C-C chemokine receptor-2 (CCR2) is the receptor for MCP1: the formation of osteoclast-like cells was inhibited by constant exposure to the CCR2 antagonist RS102895, in part by decreasing expression of RANK, the receptor for RANKL.
abstract_unstemmed	In vitro osteoclast methods require constant treatment with macrophage colony stimulating factor (M-CSF) to support precursor survival and addition of the differentiation agent receptor activator of NF-κB ligand (RANKL). Constant exposure to granulocyte macrophage colony stimulating factor (GM-CSF) suppresses human osteoclast formation in vitro. Addition of the chemokine monocyte chemotactic protein-1 (MCP1) to such cultures dramatically increases osteoclast formation and overcomes GM-CSF mediated suppression. We investigated the effect of M-CSF, GM-CSF and the combination of M-CSF and GM-CSF treatment on the expression of chemokines in human CD14+ cells in culture. Of assayed chemokines, MCP1 was the most abundant in terms of mRNA transcript and protein in M-CSF treated cultures and was suppressed by GM-CSF. MCP1 protein accumulated up to 50 ng/mL in culture medium, greatly exceeding other assayed chemokines. C-C chemokine receptor-2 (CCR2) is the receptor for MCP1: the formation of osteoclast-like cells was inhibited by constant exposure to the CCR2 antagonist RS102895, in part by decreasing expression of RANK, the receptor for RANKL.
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title_short	Monocyte Chemotactic Protein-1 (MCP1) Accumulation in Human Osteoclast Precursor Cultures
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Monocyte Chemotactic Protein-1 (MCP1) Accumulation in Human Osteoclast Precursor Cultures

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