Genetic Co-Administration of Soluble PD-1 Ectodomains Modifies Immune Responses against Influenza A Virus Induced by DNA Vaccination

Due to the low efficacy and the need for seasonal adaptation of currently licensed influenza A vaccines, the importance of alternative vaccination strategies is increasingly recognized. Considering that DNA vaccines can be rapidly manufactured and readily adapted with novel antigen sequences, geneti...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Pierre Tannig [verfasserIn] Antonia Sophia Peter [verfasserIn] Dennis Lapuente [verfasserIn] Stephan Klessing [verfasserIn] Anna Schmidt [verfasserIn] Dominik Damm [verfasserIn] Matthias Tenbusch [verfasserIn] Klaus Überla [verfasserIn] Vladimir Temchura [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	influenza A DNA vaccine DNA adjuvants checkpoint blockade soluble PD-1 soluble PD-L1

Übergeordnetes Werk:	In: Vaccines - MDPI AG, 2013, 8(2020), 4, p 570
Übergeordnetes Werk:	volume:8 ; year:2020 ; number:4, p 570

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/vaccines8040570

Katalog-ID:	DOAJ079065104

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allfields	10.3390/vaccines8040570 doi (DE-627)DOAJ079065104 (DE-599)DOAJ80074c0243aa4d8b920c3d5c3b9515c0 DE-627 ger DE-627 rakwb eng Pierre Tannig verfasserin aut Genetic Co-Administration of Soluble PD-1 Ectodomains Modifies Immune Responses against Influenza A Virus Induced by DNA Vaccination 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Due to the low efficacy and the need for seasonal adaptation of currently licensed influenza A vaccines, the importance of alternative vaccination strategies is increasingly recognized. Considering that DNA vaccines can be rapidly manufactured and readily adapted with novel antigen sequences, genetic vaccination is a promising immunization platform. However, the applicability of different genetic adjuvants to this approach still represents a complex challenge. Immune checkpoints are a class of molecules involved in adaptive immune responses and germinal center reactions. In this study, we immunized mice by intramuscular electroporation with a DNA-vaccine encoding hemagglutinin (HA) and nucleoprotein (NP) of the influenza A virus. The DNA-vaccine was applied either alone or in combination with genetic adjuvants encoding the soluble ectodomains of programmed cell death protein-1 (sPD-1) or its ligand (sPD-L1). Co-administration of genetic checkpoint adjuvants did not significantly alter immune responses against NP. In contrast, sPD-1 co-electroporation elevated HA-specific CD4<sup<+</sup< T cell responses, decreased regulatory CD4<sup<+</sup< T cell pools, and modulated the IgG2a-biased HA antibody pattern towards an isotype-balanced IgG response with a trend to higher influenza neutralization in vitro. Taken together, our data demonstrate that a genetic DNA-adjuvant encoding soluble ectodomains of sPD-1 was able to modulate immune responses induced by a co-administered influenza DNA vaccine. influenza A DNA vaccine DNA adjuvants checkpoint blockade soluble PD-1 soluble PD-L1 Medicine R Antonia Sophia Peter verfasserin aut Dennis Lapuente verfasserin aut Stephan Klessing verfasserin aut Anna Schmidt verfasserin aut Dominik Damm verfasserin aut Matthias Tenbusch verfasserin aut Klaus Überla verfasserin aut Vladimir Temchura verfasserin aut In Vaccines MDPI AG, 2013 8(2020), 4, p 570 (DE-627)736559205 (DE-600)2703319-3 2076393X nnns volume:8 year:2020 number:4, p 570 https://doi.org/10.3390/vaccines8040570 kostenfrei https://doaj.org/article/80074c0243aa4d8b920c3d5c3b9515c0 kostenfrei https://www.mdpi.com/2076-393X/8/4/570 kostenfrei https://doaj.org/toc/2076-393X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2020 4, p 570
spelling	10.3390/vaccines8040570 doi (DE-627)DOAJ079065104 (DE-599)DOAJ80074c0243aa4d8b920c3d5c3b9515c0 DE-627 ger DE-627 rakwb eng Pierre Tannig verfasserin aut Genetic Co-Administration of Soluble PD-1 Ectodomains Modifies Immune Responses against Influenza A Virus Induced by DNA Vaccination 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Due to the low efficacy and the need for seasonal adaptation of currently licensed influenza A vaccines, the importance of alternative vaccination strategies is increasingly recognized. Considering that DNA vaccines can be rapidly manufactured and readily adapted with novel antigen sequences, genetic vaccination is a promising immunization platform. However, the applicability of different genetic adjuvants to this approach still represents a complex challenge. Immune checkpoints are a class of molecules involved in adaptive immune responses and germinal center reactions. In this study, we immunized mice by intramuscular electroporation with a DNA-vaccine encoding hemagglutinin (HA) and nucleoprotein (NP) of the influenza A virus. The DNA-vaccine was applied either alone or in combination with genetic adjuvants encoding the soluble ectodomains of programmed cell death protein-1 (sPD-1) or its ligand (sPD-L1). Co-administration of genetic checkpoint adjuvants did not significantly alter immune responses against NP. In contrast, sPD-1 co-electroporation elevated HA-specific CD4<sup<+</sup< T cell responses, decreased regulatory CD4<sup<+</sup< T cell pools, and modulated the IgG2a-biased HA antibody pattern towards an isotype-balanced IgG response with a trend to higher influenza neutralization in vitro. Taken together, our data demonstrate that a genetic DNA-adjuvant encoding soluble ectodomains of sPD-1 was able to modulate immune responses induced by a co-administered influenza DNA vaccine. influenza A DNA vaccine DNA adjuvants checkpoint blockade soluble PD-1 soluble PD-L1 Medicine R Antonia Sophia Peter verfasserin aut Dennis Lapuente verfasserin aut Stephan Klessing verfasserin aut Anna Schmidt verfasserin aut Dominik Damm verfasserin aut Matthias Tenbusch verfasserin aut Klaus Überla verfasserin aut Vladimir Temchura verfasserin aut In Vaccines MDPI AG, 2013 8(2020), 4, p 570 (DE-627)736559205 (DE-600)2703319-3 2076393X nnns volume:8 year:2020 number:4, p 570 https://doi.org/10.3390/vaccines8040570 kostenfrei https://doaj.org/article/80074c0243aa4d8b920c3d5c3b9515c0 kostenfrei https://www.mdpi.com/2076-393X/8/4/570 kostenfrei https://doaj.org/toc/2076-393X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2020 4, p 570
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allfieldsGer	10.3390/vaccines8040570 doi (DE-627)DOAJ079065104 (DE-599)DOAJ80074c0243aa4d8b920c3d5c3b9515c0 DE-627 ger DE-627 rakwb eng Pierre Tannig verfasserin aut Genetic Co-Administration of Soluble PD-1 Ectodomains Modifies Immune Responses against Influenza A Virus Induced by DNA Vaccination 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Due to the low efficacy and the need for seasonal adaptation of currently licensed influenza A vaccines, the importance of alternative vaccination strategies is increasingly recognized. Considering that DNA vaccines can be rapidly manufactured and readily adapted with novel antigen sequences, genetic vaccination is a promising immunization platform. However, the applicability of different genetic adjuvants to this approach still represents a complex challenge. Immune checkpoints are a class of molecules involved in adaptive immune responses and germinal center reactions. In this study, we immunized mice by intramuscular electroporation with a DNA-vaccine encoding hemagglutinin (HA) and nucleoprotein (NP) of the influenza A virus. The DNA-vaccine was applied either alone or in combination with genetic adjuvants encoding the soluble ectodomains of programmed cell death protein-1 (sPD-1) or its ligand (sPD-L1). Co-administration of genetic checkpoint adjuvants did not significantly alter immune responses against NP. In contrast, sPD-1 co-electroporation elevated HA-specific CD4<sup<+</sup< T cell responses, decreased regulatory CD4<sup<+</sup< T cell pools, and modulated the IgG2a-biased HA antibody pattern towards an isotype-balanced IgG response with a trend to higher influenza neutralization in vitro. Taken together, our data demonstrate that a genetic DNA-adjuvant encoding soluble ectodomains of sPD-1 was able to modulate immune responses induced by a co-administered influenza DNA vaccine. influenza A DNA vaccine DNA adjuvants checkpoint blockade soluble PD-1 soluble PD-L1 Medicine R Antonia Sophia Peter verfasserin aut Dennis Lapuente verfasserin aut Stephan Klessing verfasserin aut Anna Schmidt verfasserin aut Dominik Damm verfasserin aut Matthias Tenbusch verfasserin aut Klaus Überla verfasserin aut Vladimir Temchura verfasserin aut In Vaccines MDPI AG, 2013 8(2020), 4, p 570 (DE-627)736559205 (DE-600)2703319-3 2076393X nnns volume:8 year:2020 number:4, p 570 https://doi.org/10.3390/vaccines8040570 kostenfrei https://doaj.org/article/80074c0243aa4d8b920c3d5c3b9515c0 kostenfrei https://www.mdpi.com/2076-393X/8/4/570 kostenfrei https://doaj.org/toc/2076-393X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2020 4, p 570
allfieldsSound	10.3390/vaccines8040570 doi (DE-627)DOAJ079065104 (DE-599)DOAJ80074c0243aa4d8b920c3d5c3b9515c0 DE-627 ger DE-627 rakwb eng Pierre Tannig verfasserin aut Genetic Co-Administration of Soluble PD-1 Ectodomains Modifies Immune Responses against Influenza A Virus Induced by DNA Vaccination 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Due to the low efficacy and the need for seasonal adaptation of currently licensed influenza A vaccines, the importance of alternative vaccination strategies is increasingly recognized. Considering that DNA vaccines can be rapidly manufactured and readily adapted with novel antigen sequences, genetic vaccination is a promising immunization platform. However, the applicability of different genetic adjuvants to this approach still represents a complex challenge. Immune checkpoints are a class of molecules involved in adaptive immune responses and germinal center reactions. In this study, we immunized mice by intramuscular electroporation with a DNA-vaccine encoding hemagglutinin (HA) and nucleoprotein (NP) of the influenza A virus. The DNA-vaccine was applied either alone or in combination with genetic adjuvants encoding the soluble ectodomains of programmed cell death protein-1 (sPD-1) or its ligand (sPD-L1). Co-administration of genetic checkpoint adjuvants did not significantly alter immune responses against NP. In contrast, sPD-1 co-electroporation elevated HA-specific CD4<sup<+</sup< T cell responses, decreased regulatory CD4<sup<+</sup< T cell pools, and modulated the IgG2a-biased HA antibody pattern towards an isotype-balanced IgG response with a trend to higher influenza neutralization in vitro. Taken together, our data demonstrate that a genetic DNA-adjuvant encoding soluble ectodomains of sPD-1 was able to modulate immune responses induced by a co-administered influenza DNA vaccine. influenza A DNA vaccine DNA adjuvants checkpoint blockade soluble PD-1 soluble PD-L1 Medicine R Antonia Sophia Peter verfasserin aut Dennis Lapuente verfasserin aut Stephan Klessing verfasserin aut Anna Schmidt verfasserin aut Dominik Damm verfasserin aut Matthias Tenbusch verfasserin aut Klaus Überla verfasserin aut Vladimir Temchura verfasserin aut In Vaccines MDPI AG, 2013 8(2020), 4, p 570 (DE-627)736559205 (DE-600)2703319-3 2076393X nnns volume:8 year:2020 number:4, p 570 https://doi.org/10.3390/vaccines8040570 kostenfrei https://doaj.org/article/80074c0243aa4d8b920c3d5c3b9515c0 kostenfrei https://www.mdpi.com/2076-393X/8/4/570 kostenfrei https://doaj.org/toc/2076-393X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2020 4, p 570
language	English
source	In Vaccines 8(2020), 4, p 570 volume:8 year:2020 number:4, p 570
sourceStr	In Vaccines 8(2020), 4, p 570 volume:8 year:2020 number:4, p 570
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authorswithroles_txt_mv	Pierre Tannig @@aut@@ Antonia Sophia Peter @@aut@@ Dennis Lapuente @@aut@@ Stephan Klessing @@aut@@ Anna Schmidt @@aut@@ Dominik Damm @@aut@@ Matthias Tenbusch @@aut@@ Klaus Überla @@aut@@ Vladimir Temchura @@aut@@
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author	Pierre Tannig
spellingShingle	Pierre Tannig misc influenza A misc DNA vaccine misc DNA adjuvants misc checkpoint blockade misc soluble PD-1 misc soluble PD-L1 misc Medicine misc R Genetic Co-Administration of Soluble PD-1 Ectodomains Modifies Immune Responses against Influenza A Virus Induced by DNA Vaccination
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topic_title	Genetic Co-Administration of Soluble PD-1 Ectodomains Modifies Immune Responses against Influenza A Virus Induced by DNA Vaccination influenza A DNA vaccine DNA adjuvants checkpoint blockade soluble PD-1 soluble PD-L1
topic	misc influenza A misc DNA vaccine misc DNA adjuvants misc checkpoint blockade misc soluble PD-1 misc soluble PD-L1 misc Medicine misc R
topic_unstemmed	misc influenza A misc DNA vaccine misc DNA adjuvants misc checkpoint blockade misc soluble PD-1 misc soluble PD-L1 misc Medicine misc R
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title	Genetic Co-Administration of Soluble PD-1 Ectodomains Modifies Immune Responses against Influenza A Virus Induced by DNA Vaccination
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title_full	Genetic Co-Administration of Soluble PD-1 Ectodomains Modifies Immune Responses against Influenza A Virus Induced by DNA Vaccination
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author_browse	Pierre Tannig Antonia Sophia Peter Dennis Lapuente Stephan Klessing Anna Schmidt Dominik Damm Matthias Tenbusch Klaus Überla Vladimir Temchura
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title_sort	genetic co-administration of soluble pd-1 ectodomains modifies immune responses against influenza a virus induced by dna vaccination
title_auth	Genetic Co-Administration of Soluble PD-1 Ectodomains Modifies Immune Responses against Influenza A Virus Induced by DNA Vaccination
abstract	Due to the low efficacy and the need for seasonal adaptation of currently licensed influenza A vaccines, the importance of alternative vaccination strategies is increasingly recognized. Considering that DNA vaccines can be rapidly manufactured and readily adapted with novel antigen sequences, genetic vaccination is a promising immunization platform. However, the applicability of different genetic adjuvants to this approach still represents a complex challenge. Immune checkpoints are a class of molecules involved in adaptive immune responses and germinal center reactions. In this study, we immunized mice by intramuscular electroporation with a DNA-vaccine encoding hemagglutinin (HA) and nucleoprotein (NP) of the influenza A virus. The DNA-vaccine was applied either alone or in combination with genetic adjuvants encoding the soluble ectodomains of programmed cell death protein-1 (sPD-1) or its ligand (sPD-L1). Co-administration of genetic checkpoint adjuvants did not significantly alter immune responses against NP. In contrast, sPD-1 co-electroporation elevated HA-specific CD4<sup<+</sup< T cell responses, decreased regulatory CD4<sup<+</sup< T cell pools, and modulated the IgG2a-biased HA antibody pattern towards an isotype-balanced IgG response with a trend to higher influenza neutralization in vitro. Taken together, our data demonstrate that a genetic DNA-adjuvant encoding soluble ectodomains of sPD-1 was able to modulate immune responses induced by a co-administered influenza DNA vaccine.
abstractGer	Due to the low efficacy and the need for seasonal adaptation of currently licensed influenza A vaccines, the importance of alternative vaccination strategies is increasingly recognized. Considering that DNA vaccines can be rapidly manufactured and readily adapted with novel antigen sequences, genetic vaccination is a promising immunization platform. However, the applicability of different genetic adjuvants to this approach still represents a complex challenge. Immune checkpoints are a class of molecules involved in adaptive immune responses and germinal center reactions. In this study, we immunized mice by intramuscular electroporation with a DNA-vaccine encoding hemagglutinin (HA) and nucleoprotein (NP) of the influenza A virus. The DNA-vaccine was applied either alone or in combination with genetic adjuvants encoding the soluble ectodomains of programmed cell death protein-1 (sPD-1) or its ligand (sPD-L1). Co-administration of genetic checkpoint adjuvants did not significantly alter immune responses against NP. In contrast, sPD-1 co-electroporation elevated HA-specific CD4<sup<+</sup< T cell responses, decreased regulatory CD4<sup<+</sup< T cell pools, and modulated the IgG2a-biased HA antibody pattern towards an isotype-balanced IgG response with a trend to higher influenza neutralization in vitro. Taken together, our data demonstrate that a genetic DNA-adjuvant encoding soluble ectodomains of sPD-1 was able to modulate immune responses induced by a co-administered influenza DNA vaccine.
abstract_unstemmed	Due to the low efficacy and the need for seasonal adaptation of currently licensed influenza A vaccines, the importance of alternative vaccination strategies is increasingly recognized. Considering that DNA vaccines can be rapidly manufactured and readily adapted with novel antigen sequences, genetic vaccination is a promising immunization platform. However, the applicability of different genetic adjuvants to this approach still represents a complex challenge. Immune checkpoints are a class of molecules involved in adaptive immune responses and germinal center reactions. In this study, we immunized mice by intramuscular electroporation with a DNA-vaccine encoding hemagglutinin (HA) and nucleoprotein (NP) of the influenza A virus. The DNA-vaccine was applied either alone or in combination with genetic adjuvants encoding the soluble ectodomains of programmed cell death protein-1 (sPD-1) or its ligand (sPD-L1). Co-administration of genetic checkpoint adjuvants did not significantly alter immune responses against NP. In contrast, sPD-1 co-electroporation elevated HA-specific CD4<sup<+</sup< T cell responses, decreased regulatory CD4<sup<+</sup< T cell pools, and modulated the IgG2a-biased HA antibody pattern towards an isotype-balanced IgG response with a trend to higher influenza neutralization in vitro. Taken together, our data demonstrate that a genetic DNA-adjuvant encoding soluble ectodomains of sPD-1 was able to modulate immune responses induced by a co-administered influenza DNA vaccine.
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title_short	Genetic Co-Administration of Soluble PD-1 Ectodomains Modifies Immune Responses against Influenza A Virus Induced by DNA Vaccination
url	https://doi.org/10.3390/vaccines8040570 https://doaj.org/article/80074c0243aa4d8b920c3d5c3b9515c0 https://www.mdpi.com/2076-393X/8/4/570 https://doaj.org/toc/2076-393X
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author2	Antonia Sophia Peter Dennis Lapuente Stephan Klessing Anna Schmidt Dominik Damm Matthias Tenbusch Klaus Überla Vladimir Temchura
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up_date	2024-07-03T21:31:17.804Z
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Genetic Co-Administration of Soluble PD-1 Ectodomains Modifies Immune Responses against Influenza A Virus Induced by DNA Vaccination

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