Near-Infrared Spectroscopy Evaluations for the Differentiation of Carbapenem-Resistant from Susceptible Enterobacteriaceae Strains

Antimicrobial Resistance (AMR) caused by Carbapenem-Resistant Enterobacteriaceae (CRE) is a global threat. Accurate identification of these bacterial species with associated AMR is critical for their management. While highly accurate methods to detect CRE are available, they are costly, timely and r...
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Gespeichert in:

Autor*in:	Bushra Alharbi [verfasserIn] Maggy Sikulu-Lord [verfasserIn] Anton Lord [verfasserIn] Hosam M. Zowawi [verfasserIn] Ella Trembizki [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	spectroscopy near infrared Enterobacteriaceae carbapenem-resistant Enterobacteriaceae

Übergeordnetes Werk:	In: Diagnostics - MDPI AG, 2012, 10(2020), 10, p 736
Übergeordnetes Werk:	volume:10 ; year:2020 ; number:10, p 736

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/diagnostics10100736

Katalog-ID:	DOAJ079203043

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allfields	10.3390/diagnostics10100736 doi (DE-627)DOAJ079203043 (DE-599)DOAJf005f5c656bb4d83a2d7fd16031dd86d DE-627 ger DE-627 rakwb eng R5-920 Bushra Alharbi verfasserin aut Near-Infrared Spectroscopy Evaluations for the Differentiation of Carbapenem-Resistant from Susceptible Enterobacteriaceae Strains 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Antimicrobial Resistance (AMR) caused by Carbapenem-Resistant Enterobacteriaceae (CRE) is a global threat. Accurate identification of these bacterial species with associated AMR is critical for their management. While highly accurate methods to detect CRE are available, they are costly, timely and require expert skills, making their application infeasible in low-resource settings. Here, we investigated the potential of Near-Infrared Spectroscopy (NIRS) for a range of applications: (i) the detection and differentiation of isolates of two pathogenic Enterobacteriaceae species, <i<Klebsiella pneumoniae</i< and <i<Escherichia coli</i<, and (ii) the differentiation of carbapenem resistant and susceptible <i<K. pneumoniae</i<. NIRS has successfully differentiated between <i<K. pneumoniae</i< and <i<E. coli</i< isolates with a predictive accuracy of 89.04% (95% CI; 88.7–89.4%). <i<K. pneumoniae</i< isolates harbouring carbapenem-resistance determinants were differentiated from susceptible <i<K. pneumoniae</i< strains with an accuracy of 85% (95% CI; 84.2–86.1%). To our knowledge, this is the largest proof of concept demonstration for the utility and feasibility of NIRS to rapidly differentiate between <i<K. pneumoniae</i< and <i<E. coli</i< as well as carbapenem-resistant <i<K. pneumoniae</i< from susceptible strains. spectroscopy near infrared Enterobacteriaceae carbapenem-resistant Enterobacteriaceae Medicine (General) Maggy Sikulu-Lord verfasserin aut Anton Lord verfasserin aut Hosam M. Zowawi verfasserin aut Ella Trembizki verfasserin aut In Diagnostics MDPI AG, 2012 10(2020), 10, p 736 (DE-627)718627814 (DE-600)2662336-5 20754418 nnns volume:10 year:2020 number:10, p 736 https://doi.org/10.3390/diagnostics10100736 kostenfrei https://doaj.org/article/f005f5c656bb4d83a2d7fd16031dd86d kostenfrei https://www.mdpi.com/2075-4418/10/10/736 kostenfrei https://doaj.org/toc/2075-4418 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2020 10, p 736
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allfieldsSound	10.3390/diagnostics10100736 doi (DE-627)DOAJ079203043 (DE-599)DOAJf005f5c656bb4d83a2d7fd16031dd86d DE-627 ger DE-627 rakwb eng R5-920 Bushra Alharbi verfasserin aut Near-Infrared Spectroscopy Evaluations for the Differentiation of Carbapenem-Resistant from Susceptible Enterobacteriaceae Strains 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Antimicrobial Resistance (AMR) caused by Carbapenem-Resistant Enterobacteriaceae (CRE) is a global threat. Accurate identification of these bacterial species with associated AMR is critical for their management. While highly accurate methods to detect CRE are available, they are costly, timely and require expert skills, making their application infeasible in low-resource settings. Here, we investigated the potential of Near-Infrared Spectroscopy (NIRS) for a range of applications: (i) the detection and differentiation of isolates of two pathogenic Enterobacteriaceae species, <i<Klebsiella pneumoniae</i< and <i<Escherichia coli</i<, and (ii) the differentiation of carbapenem resistant and susceptible <i<K. pneumoniae</i<. NIRS has successfully differentiated between <i<K. pneumoniae</i< and <i<E. coli</i< isolates with a predictive accuracy of 89.04% (95% CI; 88.7–89.4%). <i<K. pneumoniae</i< isolates harbouring carbapenem-resistance determinants were differentiated from susceptible <i<K. pneumoniae</i< strains with an accuracy of 85% (95% CI; 84.2–86.1%). To our knowledge, this is the largest proof of concept demonstration for the utility and feasibility of NIRS to rapidly differentiate between <i<K. pneumoniae</i< and <i<E. coli</i< as well as carbapenem-resistant <i<K. pneumoniae</i< from susceptible strains. spectroscopy near infrared Enterobacteriaceae carbapenem-resistant Enterobacteriaceae Medicine (General) Maggy Sikulu-Lord verfasserin aut Anton Lord verfasserin aut Hosam M. Zowawi verfasserin aut Ella Trembizki verfasserin aut In Diagnostics MDPI AG, 2012 10(2020), 10, p 736 (DE-627)718627814 (DE-600)2662336-5 20754418 nnns volume:10 year:2020 number:10, p 736 https://doi.org/10.3390/diagnostics10100736 kostenfrei https://doaj.org/article/f005f5c656bb4d83a2d7fd16031dd86d kostenfrei https://www.mdpi.com/2075-4418/10/10/736 kostenfrei https://doaj.org/toc/2075-4418 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2020 10, p 736
language	English
source	In Diagnostics 10(2020), 10, p 736 volume:10 year:2020 number:10, p 736
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callnumber-first	R - Medicine
author	Bushra Alharbi
spellingShingle	Bushra Alharbi misc R5-920 misc spectroscopy misc near infrared misc Enterobacteriaceae misc carbapenem-resistant Enterobacteriaceae misc Medicine (General) Near-Infrared Spectroscopy Evaluations for the Differentiation of Carbapenem-Resistant from Susceptible Enterobacteriaceae Strains
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topic_title	R5-920 Near-Infrared Spectroscopy Evaluations for the Differentiation of Carbapenem-Resistant from Susceptible Enterobacteriaceae Strains spectroscopy near infrared Enterobacteriaceae carbapenem-resistant Enterobacteriaceae
topic	misc R5-920 misc spectroscopy misc near infrared misc Enterobacteriaceae misc carbapenem-resistant Enterobacteriaceae misc Medicine (General)
topic_unstemmed	misc R5-920 misc spectroscopy misc near infrared misc Enterobacteriaceae misc carbapenem-resistant Enterobacteriaceae misc Medicine (General)
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title	Near-Infrared Spectroscopy Evaluations for the Differentiation of Carbapenem-Resistant from Susceptible Enterobacteriaceae Strains
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title_full	Near-Infrared Spectroscopy Evaluations for the Differentiation of Carbapenem-Resistant from Susceptible Enterobacteriaceae Strains
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title_sort	near-infrared spectroscopy evaluations for the differentiation of carbapenem-resistant from susceptible enterobacteriaceae strains
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title_auth	Near-Infrared Spectroscopy Evaluations for the Differentiation of Carbapenem-Resistant from Susceptible Enterobacteriaceae Strains
abstract	Antimicrobial Resistance (AMR) caused by Carbapenem-Resistant Enterobacteriaceae (CRE) is a global threat. Accurate identification of these bacterial species with associated AMR is critical for their management. While highly accurate methods to detect CRE are available, they are costly, timely and require expert skills, making their application infeasible in low-resource settings. Here, we investigated the potential of Near-Infrared Spectroscopy (NIRS) for a range of applications: (i) the detection and differentiation of isolates of two pathogenic Enterobacteriaceae species, <i<Klebsiella pneumoniae</i< and <i<Escherichia coli</i<, and (ii) the differentiation of carbapenem resistant and susceptible <i<K. pneumoniae</i<. NIRS has successfully differentiated between <i<K. pneumoniae</i< and <i<E. coli</i< isolates with a predictive accuracy of 89.04% (95% CI; 88.7–89.4%). <i<K. pneumoniae</i< isolates harbouring carbapenem-resistance determinants were differentiated from susceptible <i<K. pneumoniae</i< strains with an accuracy of 85% (95% CI; 84.2–86.1%). To our knowledge, this is the largest proof of concept demonstration for the utility and feasibility of NIRS to rapidly differentiate between <i<K. pneumoniae</i< and <i<E. coli</i< as well as carbapenem-resistant <i<K. pneumoniae</i< from susceptible strains.
abstractGer	Antimicrobial Resistance (AMR) caused by Carbapenem-Resistant Enterobacteriaceae (CRE) is a global threat. Accurate identification of these bacterial species with associated AMR is critical for their management. While highly accurate methods to detect CRE are available, they are costly, timely and require expert skills, making their application infeasible in low-resource settings. Here, we investigated the potential of Near-Infrared Spectroscopy (NIRS) for a range of applications: (i) the detection and differentiation of isolates of two pathogenic Enterobacteriaceae species, <i<Klebsiella pneumoniae</i< and <i<Escherichia coli</i<, and (ii) the differentiation of carbapenem resistant and susceptible <i<K. pneumoniae</i<. NIRS has successfully differentiated between <i<K. pneumoniae</i< and <i<E. coli</i< isolates with a predictive accuracy of 89.04% (95% CI; 88.7–89.4%). <i<K. pneumoniae</i< isolates harbouring carbapenem-resistance determinants were differentiated from susceptible <i<K. pneumoniae</i< strains with an accuracy of 85% (95% CI; 84.2–86.1%). To our knowledge, this is the largest proof of concept demonstration for the utility and feasibility of NIRS to rapidly differentiate between <i<K. pneumoniae</i< and <i<E. coli</i< as well as carbapenem-resistant <i<K. pneumoniae</i< from susceptible strains.
abstract_unstemmed	Antimicrobial Resistance (AMR) caused by Carbapenem-Resistant Enterobacteriaceae (CRE) is a global threat. Accurate identification of these bacterial species with associated AMR is critical for their management. While highly accurate methods to detect CRE are available, they are costly, timely and require expert skills, making their application infeasible in low-resource settings. Here, we investigated the potential of Near-Infrared Spectroscopy (NIRS) for a range of applications: (i) the detection and differentiation of isolates of two pathogenic Enterobacteriaceae species, <i<Klebsiella pneumoniae</i< and <i<Escherichia coli</i<, and (ii) the differentiation of carbapenem resistant and susceptible <i<K. pneumoniae</i<. NIRS has successfully differentiated between <i<K. pneumoniae</i< and <i<E. coli</i< isolates with a predictive accuracy of 89.04% (95% CI; 88.7–89.4%). <i<K. pneumoniae</i< isolates harbouring carbapenem-resistance determinants were differentiated from susceptible <i<K. pneumoniae</i< strains with an accuracy of 85% (95% CI; 84.2–86.1%). To our knowledge, this is the largest proof of concept demonstration for the utility and feasibility of NIRS to rapidly differentiate between <i<K. pneumoniae</i< and <i<E. coli</i< as well as carbapenem-resistant <i<K. pneumoniae</i< from susceptible strains.
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title_short	Near-Infrared Spectroscopy Evaluations for the Differentiation of Carbapenem-Resistant from Susceptible Enterobacteriaceae Strains
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Near-Infrared Spectroscopy Evaluations for the Differentiation of Carbapenem-Resistant from Susceptible Enterobacteriaceae Strains

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