Protective Effect of Nasal Colonisation with <i<∆cps/piaA</i< and <i<∆cps/proABC</i<<i<Streptococcus pneumoniae</i< Strains against Recolonisation and Invasive Infection

Rationale: Nasopharyngeal administration of live virulence-attenuated <i<Streptococcus pneumoniae</i< strains is a potential novel preventative strategy. One target for creating reduced virulence <i<S. pneumoniae</i< strains is the capsule, but loss of the capsule reduces the duration of <i<S. pneumoniae</i< colonisation in mice which could impair protective efficacy against subsequent infection. Objectives: To assess protective efficacy of nasopharyngeal administration of unencapsulated <i<S. pneumoniae</i< strains in murine infection models. Methods: Strains containing <i<cps</i< locus deletions combined with the <i<S. pneumoniae</i< virulence factors <i<psaA</i< (reduces colonisation) or <i<proABC</i< (no effect on colonisation) were constructed and their virulence phenotypes and ability to prevent recolonisation or invasive infection assessed using mouse infection models. Serological responses to colonisation were compared between strains using ELISAs, immunoblots and 254 <i<S. pneumoniae</i< protein antigen array. Measurements and Main Results: The <i<∆cps/piaA</i< and <i<∆cps/proABC</i< strains were strongly attenuated in virulence in both invasive infection models and had a reduced ability to colonise the nasopharynx. ELISAs, immunoblots and protein arrays showed colonisation with either strain stimulated weaker serological responses than the wild type strain. Mice previously colonised with these strains were protected against septicaemic pneumonia but, unlike mice colonised with the wild type strain, not against <i<S. pneumoniae</i< recolonisation. Conclusions: Colonisation with the <i<∆cps/piaA</i< and <i<∆cps/proABC</i< strains prevented subsequent septicaemia, but in contrast, to published data for encapsulated double mutant strains they did not prevent recolonisation with <i<S. pneumoniae</i<. These data suggest targeting the <i<cps</i< locus is a less effective option for creating live attenuated strains that prevent <i<S. pneumoniae</i< infections. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Elisa Ramos-Sevillano [verfasserIn] Giuseppe Ercoli [verfasserIn] José Afonso Guerra-Assunção [verfasserIn] Philip Felgner [verfasserIn] Rafael Ramiro de Assis [verfasserIn] Rie Nakajima [verfasserIn] David Goldblatt [verfasserIn] Kevin Kweku Adjei Tetteh [verfasserIn] Robert Simon Heyderman [verfasserIn] Stephen Brian Gordon [verfasserIn] Daniela Mulari Ferreria [verfasserIn] Jeremy Stuart Brown [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	capsule vaccine colonisation

Übergeordnetes Werk:	In: Vaccines - MDPI AG, 2013, 9(2021), 3, p 261
Übergeordnetes Werk:	volume:9 ; year:2021 ; number:3, p 261

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/vaccines9030261

Katalog-ID:	DOAJ079235166

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100	0		\|a Elisa Ramos-Sevillano \|e verfasserin \|4 aut
245	1	0	\|a Protective Effect of Nasal Colonisation with <i<∆cps/piaA</i< and <i<∆cps/proABC</i<<i<Streptococcus pneumoniae</i< Strains against Recolonisation and Invasive Infection
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520			\|a Rationale: Nasopharyngeal administration of live virulence-attenuated <i<Streptococcus pneumoniae</i< strains is a potential novel preventative strategy. One target for creating reduced virulence <i<S. pneumoniae</i< strains is the capsule, but loss of the capsule reduces the duration of <i<S. pneumoniae</i< colonisation in mice which could impair protective efficacy against subsequent infection. Objectives: To assess protective efficacy of nasopharyngeal administration of unencapsulated <i<S. pneumoniae</i< strains in murine infection models. Methods: Strains containing <i<cps</i< locus deletions combined with the <i<S. pneumoniae</i< virulence factors <i<psaA</i< (reduces colonisation) or <i<proABC</i< (no effect on colonisation) were constructed and their virulence phenotypes and ability to prevent recolonisation or invasive infection assessed using mouse infection models. Serological responses to colonisation were compared between strains using ELISAs, immunoblots and 254 <i<S. pneumoniae</i< protein antigen array. Measurements and Main Results: The <i<∆cps/piaA</i< and <i<∆cps/proABC</i< strains were strongly attenuated in virulence in both invasive infection models and had a reduced ability to colonise the nasopharynx. ELISAs, immunoblots and protein arrays showed colonisation with either strain stimulated weaker serological responses than the wild type strain. Mice previously colonised with these strains were protected against septicaemic pneumonia but, unlike mice colonised with the wild type strain, not against <i<S. pneumoniae</i< recolonisation. Conclusions: Colonisation with the <i<∆cps/piaA</i< and <i<∆cps/proABC</i< strains prevented subsequent septicaemia, but in contrast, to published data for encapsulated double mutant strains they did not prevent recolonisation with <i<S. pneumoniae</i<. These data suggest targeting the <i<cps</i< locus is a less effective option for creating live attenuated strains that prevent <i<S. pneumoniae</i< infections.
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Indexfelder

author_variant	e r s ers g e ge j a g a jaga p f pf r r d a rrda r n rn d g dg k k a t kkat r s h rsh s b g sbg d m f dmf j s b jsb
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publishDate	2021
allfields	10.3390/vaccines9030261 doi (DE-627)DOAJ079235166 (DE-599)DOAJ49a7fad3616a46729a4e6aada6193bc7 DE-627 ger DE-627 rakwb eng Elisa Ramos-Sevillano verfasserin aut Protective Effect of Nasal Colonisation with <i<∆cps/piaA</i< and <i<∆cps/proABC</i<<i<Streptococcus pneumoniae</i< Strains against Recolonisation and Invasive Infection 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Rationale: Nasopharyngeal administration of live virulence-attenuated <i<Streptococcus pneumoniae</i< strains is a potential novel preventative strategy. One target for creating reduced virulence <i<S. pneumoniae</i< strains is the capsule, but loss of the capsule reduces the duration of <i<S. pneumoniae</i< colonisation in mice which could impair protective efficacy against subsequent infection. Objectives: To assess protective efficacy of nasopharyngeal administration of unencapsulated <i<S. pneumoniae</i< strains in murine infection models. Methods: Strains containing <i<cps</i< locus deletions combined with the <i<S. pneumoniae</i< virulence factors <i<psaA</i< (reduces colonisation) or <i<proABC</i< (no effect on colonisation) were constructed and their virulence phenotypes and ability to prevent recolonisation or invasive infection assessed using mouse infection models. Serological responses to colonisation were compared between strains using ELISAs, immunoblots and 254 <i<S. pneumoniae</i< protein antigen array. Measurements and Main Results: The <i<∆cps/piaA</i< and <i<∆cps/proABC</i< strains were strongly attenuated in virulence in both invasive infection models and had a reduced ability to colonise the nasopharynx. ELISAs, immunoblots and protein arrays showed colonisation with either strain stimulated weaker serological responses than the wild type strain. Mice previously colonised with these strains were protected against septicaemic pneumonia but, unlike mice colonised with the wild type strain, not against <i<S. pneumoniae</i< recolonisation. Conclusions: Colonisation with the <i<∆cps/piaA</i< and <i<∆cps/proABC</i< strains prevented subsequent septicaemia, but in contrast, to published data for encapsulated double mutant strains they did not prevent recolonisation with <i<S. pneumoniae</i<. These data suggest targeting the <i<cps</i< locus is a less effective option for creating live attenuated strains that prevent <i<S. pneumoniae</i< infections. <i<Streptococcus pneumoniae</i< capsule vaccine <i<psaA</i< <i<proABC</i< colonisation Medicine R Giuseppe Ercoli verfasserin aut José Afonso Guerra-Assunção verfasserin aut Philip Felgner verfasserin aut Rafael Ramiro de Assis verfasserin aut Rie Nakajima verfasserin aut David Goldblatt verfasserin aut Kevin Kweku Adjei Tetteh verfasserin aut Robert Simon Heyderman verfasserin aut Stephen Brian Gordon verfasserin aut Daniela Mulari Ferreria verfasserin aut Jeremy Stuart Brown verfasserin aut In Vaccines MDPI AG, 2013 9(2021), 3, p 261 (DE-627)736559205 (DE-600)2703319-3 2076393X nnns volume:9 year:2021 number:3, p 261 https://doi.org/10.3390/vaccines9030261 kostenfrei https://doaj.org/article/49a7fad3616a46729a4e6aada6193bc7 kostenfrei https://www.mdpi.com/2076-393X/9/3/261 kostenfrei https://doaj.org/toc/2076-393X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 3, p 261
spelling	10.3390/vaccines9030261 doi (DE-627)DOAJ079235166 (DE-599)DOAJ49a7fad3616a46729a4e6aada6193bc7 DE-627 ger DE-627 rakwb eng Elisa Ramos-Sevillano verfasserin aut Protective Effect of Nasal Colonisation with <i<∆cps/piaA</i< and <i<∆cps/proABC</i<<i<Streptococcus pneumoniae</i< Strains against Recolonisation and Invasive Infection 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Rationale: Nasopharyngeal administration of live virulence-attenuated <i<Streptococcus pneumoniae</i< strains is a potential novel preventative strategy. One target for creating reduced virulence <i<S. pneumoniae</i< strains is the capsule, but loss of the capsule reduces the duration of <i<S. pneumoniae</i< colonisation in mice which could impair protective efficacy against subsequent infection. Objectives: To assess protective efficacy of nasopharyngeal administration of unencapsulated <i<S. pneumoniae</i< strains in murine infection models. Methods: Strains containing <i<cps</i< locus deletions combined with the <i<S. pneumoniae</i< virulence factors <i<psaA</i< (reduces colonisation) or <i<proABC</i< (no effect on colonisation) were constructed and their virulence phenotypes and ability to prevent recolonisation or invasive infection assessed using mouse infection models. Serological responses to colonisation were compared between strains using ELISAs, immunoblots and 254 <i<S. pneumoniae</i< protein antigen array. Measurements and Main Results: The <i<∆cps/piaA</i< and <i<∆cps/proABC</i< strains were strongly attenuated in virulence in both invasive infection models and had a reduced ability to colonise the nasopharynx. ELISAs, immunoblots and protein arrays showed colonisation with either strain stimulated weaker serological responses than the wild type strain. Mice previously colonised with these strains were protected against septicaemic pneumonia but, unlike mice colonised with the wild type strain, not against <i<S. pneumoniae</i< recolonisation. Conclusions: Colonisation with the <i<∆cps/piaA</i< and <i<∆cps/proABC</i< strains prevented subsequent septicaemia, but in contrast, to published data for encapsulated double mutant strains they did not prevent recolonisation with <i<S. pneumoniae</i<. These data suggest targeting the <i<cps</i< locus is a less effective option for creating live attenuated strains that prevent <i<S. pneumoniae</i< infections. <i<Streptococcus pneumoniae</i< capsule vaccine <i<psaA</i< <i<proABC</i< colonisation Medicine R Giuseppe Ercoli verfasserin aut José Afonso Guerra-Assunção verfasserin aut Philip Felgner verfasserin aut Rafael Ramiro de Assis verfasserin aut Rie Nakajima verfasserin aut David Goldblatt verfasserin aut Kevin Kweku Adjei Tetteh verfasserin aut Robert Simon Heyderman verfasserin aut Stephen Brian Gordon verfasserin aut Daniela Mulari Ferreria verfasserin aut Jeremy Stuart Brown verfasserin aut In Vaccines MDPI AG, 2013 9(2021), 3, p 261 (DE-627)736559205 (DE-600)2703319-3 2076393X nnns volume:9 year:2021 number:3, p 261 https://doi.org/10.3390/vaccines9030261 kostenfrei https://doaj.org/article/49a7fad3616a46729a4e6aada6193bc7 kostenfrei https://www.mdpi.com/2076-393X/9/3/261 kostenfrei https://doaj.org/toc/2076-393X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 3, p 261
allfields_unstemmed	10.3390/vaccines9030261 doi (DE-627)DOAJ079235166 (DE-599)DOAJ49a7fad3616a46729a4e6aada6193bc7 DE-627 ger DE-627 rakwb eng Elisa Ramos-Sevillano verfasserin aut Protective Effect of Nasal Colonisation with <i<∆cps/piaA</i< and <i<∆cps/proABC</i<<i<Streptococcus pneumoniae</i< Strains against Recolonisation and Invasive Infection 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Rationale: Nasopharyngeal administration of live virulence-attenuated <i<Streptococcus pneumoniae</i< strains is a potential novel preventative strategy. One target for creating reduced virulence <i<S. pneumoniae</i< strains is the capsule, but loss of the capsule reduces the duration of <i<S. pneumoniae</i< colonisation in mice which could impair protective efficacy against subsequent infection. Objectives: To assess protective efficacy of nasopharyngeal administration of unencapsulated <i<S. pneumoniae</i< strains in murine infection models. Methods: Strains containing <i<cps</i< locus deletions combined with the <i<S. pneumoniae</i< virulence factors <i<psaA</i< (reduces colonisation) or <i<proABC</i< (no effect on colonisation) were constructed and their virulence phenotypes and ability to prevent recolonisation or invasive infection assessed using mouse infection models. Serological responses to colonisation were compared between strains using ELISAs, immunoblots and 254 <i<S. pneumoniae</i< protein antigen array. Measurements and Main Results: The <i<∆cps/piaA</i< and <i<∆cps/proABC</i< strains were strongly attenuated in virulence in both invasive infection models and had a reduced ability to colonise the nasopharynx. ELISAs, immunoblots and protein arrays showed colonisation with either strain stimulated weaker serological responses than the wild type strain. Mice previously colonised with these strains were protected against septicaemic pneumonia but, unlike mice colonised with the wild type strain, not against <i<S. pneumoniae</i< recolonisation. Conclusions: Colonisation with the <i<∆cps/piaA</i< and <i<∆cps/proABC</i< strains prevented subsequent septicaemia, but in contrast, to published data for encapsulated double mutant strains they did not prevent recolonisation with <i<S. pneumoniae</i<. These data suggest targeting the <i<cps</i< locus is a less effective option for creating live attenuated strains that prevent <i<S. pneumoniae</i< infections. <i<Streptococcus pneumoniae</i< capsule vaccine <i<psaA</i< <i<proABC</i< colonisation Medicine R Giuseppe Ercoli verfasserin aut José Afonso Guerra-Assunção verfasserin aut Philip Felgner verfasserin aut Rafael Ramiro de Assis verfasserin aut Rie Nakajima verfasserin aut David Goldblatt verfasserin aut Kevin Kweku Adjei Tetteh verfasserin aut Robert Simon Heyderman verfasserin aut Stephen Brian Gordon verfasserin aut Daniela Mulari Ferreria verfasserin aut Jeremy Stuart Brown verfasserin aut In Vaccines MDPI AG, 2013 9(2021), 3, p 261 (DE-627)736559205 (DE-600)2703319-3 2076393X nnns volume:9 year:2021 number:3, p 261 https://doi.org/10.3390/vaccines9030261 kostenfrei https://doaj.org/article/49a7fad3616a46729a4e6aada6193bc7 kostenfrei https://www.mdpi.com/2076-393X/9/3/261 kostenfrei https://doaj.org/toc/2076-393X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 3, p 261
allfieldsGer	10.3390/vaccines9030261 doi (DE-627)DOAJ079235166 (DE-599)DOAJ49a7fad3616a46729a4e6aada6193bc7 DE-627 ger DE-627 rakwb eng Elisa Ramos-Sevillano verfasserin aut Protective Effect of Nasal Colonisation with <i<∆cps/piaA</i< and <i<∆cps/proABC</i<<i<Streptococcus pneumoniae</i< Strains against Recolonisation and Invasive Infection 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Rationale: Nasopharyngeal administration of live virulence-attenuated <i<Streptococcus pneumoniae</i< strains is a potential novel preventative strategy. One target for creating reduced virulence <i<S. pneumoniae</i< strains is the capsule, but loss of the capsule reduces the duration of <i<S. pneumoniae</i< colonisation in mice which could impair protective efficacy against subsequent infection. Objectives: To assess protective efficacy of nasopharyngeal administration of unencapsulated <i<S. pneumoniae</i< strains in murine infection models. Methods: Strains containing <i<cps</i< locus deletions combined with the <i<S. pneumoniae</i< virulence factors <i<psaA</i< (reduces colonisation) or <i<proABC</i< (no effect on colonisation) were constructed and their virulence phenotypes and ability to prevent recolonisation or invasive infection assessed using mouse infection models. Serological responses to colonisation were compared between strains using ELISAs, immunoblots and 254 <i<S. pneumoniae</i< protein antigen array. Measurements and Main Results: The <i<∆cps/piaA</i< and <i<∆cps/proABC</i< strains were strongly attenuated in virulence in both invasive infection models and had a reduced ability to colonise the nasopharynx. ELISAs, immunoblots and protein arrays showed colonisation with either strain stimulated weaker serological responses than the wild type strain. Mice previously colonised with these strains were protected against septicaemic pneumonia but, unlike mice colonised with the wild type strain, not against <i<S. pneumoniae</i< recolonisation. Conclusions: Colonisation with the <i<∆cps/piaA</i< and <i<∆cps/proABC</i< strains prevented subsequent septicaemia, but in contrast, to published data for encapsulated double mutant strains they did not prevent recolonisation with <i<S. pneumoniae</i<. These data suggest targeting the <i<cps</i< locus is a less effective option for creating live attenuated strains that prevent <i<S. pneumoniae</i< infections. <i<Streptococcus pneumoniae</i< capsule vaccine <i<psaA</i< <i<proABC</i< colonisation Medicine R Giuseppe Ercoli verfasserin aut José Afonso Guerra-Assunção verfasserin aut Philip Felgner verfasserin aut Rafael Ramiro de Assis verfasserin aut Rie Nakajima verfasserin aut David Goldblatt verfasserin aut Kevin Kweku Adjei Tetteh verfasserin aut Robert Simon Heyderman verfasserin aut Stephen Brian Gordon verfasserin aut Daniela Mulari Ferreria verfasserin aut Jeremy Stuart Brown verfasserin aut In Vaccines MDPI AG, 2013 9(2021), 3, p 261 (DE-627)736559205 (DE-600)2703319-3 2076393X nnns volume:9 year:2021 number:3, p 261 https://doi.org/10.3390/vaccines9030261 kostenfrei https://doaj.org/article/49a7fad3616a46729a4e6aada6193bc7 kostenfrei https://www.mdpi.com/2076-393X/9/3/261 kostenfrei https://doaj.org/toc/2076-393X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 3, p 261
allfieldsSound	10.3390/vaccines9030261 doi (DE-627)DOAJ079235166 (DE-599)DOAJ49a7fad3616a46729a4e6aada6193bc7 DE-627 ger DE-627 rakwb eng Elisa Ramos-Sevillano verfasserin aut Protective Effect of Nasal Colonisation with <i<∆cps/piaA</i< and <i<∆cps/proABC</i<<i<Streptococcus pneumoniae</i< Strains against Recolonisation and Invasive Infection 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Rationale: Nasopharyngeal administration of live virulence-attenuated <i<Streptococcus pneumoniae</i< strains is a potential novel preventative strategy. One target for creating reduced virulence <i<S. pneumoniae</i< strains is the capsule, but loss of the capsule reduces the duration of <i<S. pneumoniae</i< colonisation in mice which could impair protective efficacy against subsequent infection. Objectives: To assess protective efficacy of nasopharyngeal administration of unencapsulated <i<S. pneumoniae</i< strains in murine infection models. Methods: Strains containing <i<cps</i< locus deletions combined with the <i<S. pneumoniae</i< virulence factors <i<psaA</i< (reduces colonisation) or <i<proABC</i< (no effect on colonisation) were constructed and their virulence phenotypes and ability to prevent recolonisation or invasive infection assessed using mouse infection models. Serological responses to colonisation were compared between strains using ELISAs, immunoblots and 254 <i<S. pneumoniae</i< protein antigen array. Measurements and Main Results: The <i<∆cps/piaA</i< and <i<∆cps/proABC</i< strains were strongly attenuated in virulence in both invasive infection models and had a reduced ability to colonise the nasopharynx. ELISAs, immunoblots and protein arrays showed colonisation with either strain stimulated weaker serological responses than the wild type strain. Mice previously colonised with these strains were protected against septicaemic pneumonia but, unlike mice colonised with the wild type strain, not against <i<S. pneumoniae</i< recolonisation. Conclusions: Colonisation with the <i<∆cps/piaA</i< and <i<∆cps/proABC</i< strains prevented subsequent septicaemia, but in contrast, to published data for encapsulated double mutant strains they did not prevent recolonisation with <i<S. pneumoniae</i<. These data suggest targeting the <i<cps</i< locus is a less effective option for creating live attenuated strains that prevent <i<S. pneumoniae</i< infections. <i<Streptococcus pneumoniae</i< capsule vaccine <i<psaA</i< <i<proABC</i< colonisation Medicine R Giuseppe Ercoli verfasserin aut José Afonso Guerra-Assunção verfasserin aut Philip Felgner verfasserin aut Rafael Ramiro de Assis verfasserin aut Rie Nakajima verfasserin aut David Goldblatt verfasserin aut Kevin Kweku Adjei Tetteh verfasserin aut Robert Simon Heyderman verfasserin aut Stephen Brian Gordon verfasserin aut Daniela Mulari Ferreria verfasserin aut Jeremy Stuart Brown verfasserin aut In Vaccines MDPI AG, 2013 9(2021), 3, p 261 (DE-627)736559205 (DE-600)2703319-3 2076393X nnns volume:9 year:2021 number:3, p 261 https://doi.org/10.3390/vaccines9030261 kostenfrei https://doaj.org/article/49a7fad3616a46729a4e6aada6193bc7 kostenfrei https://www.mdpi.com/2076-393X/9/3/261 kostenfrei https://doaj.org/toc/2076-393X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 3, p 261
language	English
source	In Vaccines 9(2021), 3, p 261 volume:9 year:2021 number:3, p 261
sourceStr	In Vaccines 9(2021), 3, p 261 volume:9 year:2021 number:3, p 261
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	<i<Streptococcus pneumoniae</i< capsule vaccine <i<psaA</i< <i<proABC</i< colonisation Medicine R
isfreeaccess_bool	true
container_title	Vaccines
authorswithroles_txt_mv	Elisa Ramos-Sevillano @@aut@@ Giuseppe Ercoli @@aut@@ José Afonso Guerra-Assunção @@aut@@ Philip Felgner @@aut@@ Rafael Ramiro de Assis @@aut@@ Rie Nakajima @@aut@@ David Goldblatt @@aut@@ Kevin Kweku Adjei Tetteh @@aut@@ Robert Simon Heyderman @@aut@@ Stephen Brian Gordon @@aut@@ Daniela Mulari Ferreria @@aut@@ Jeremy Stuart Brown @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	736559205
id	DOAJ079235166
language_de	englisch
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One target for creating reduced virulence <i<S. pneumoniae</i< strains is the capsule, but loss of the capsule reduces the duration of <i<S. pneumoniae</i< colonisation in mice which could impair protective efficacy against subsequent infection. Objectives: To assess protective efficacy of nasopharyngeal administration of unencapsulated <i<S. pneumoniae</i< strains in murine infection models. Methods: Strains containing <i<cps</i< locus deletions combined with the <i<S. pneumoniae</i< virulence factors <i<psaA</i< (reduces colonisation) or <i<proABC</i< (no effect on colonisation) were constructed and their virulence phenotypes and ability to prevent recolonisation or invasive infection assessed using mouse infection models. Serological responses to colonisation were compared between strains using ELISAs, immunoblots and 254 <i<S. pneumoniae</i< protein antigen array. Measurements and Main Results: The <i<∆cps/piaA</i< and <i<∆cps/proABC</i< strains were strongly attenuated in virulence in both invasive infection models and had a reduced ability to colonise the nasopharynx. ELISAs, immunoblots and protein arrays showed colonisation with either strain stimulated weaker serological responses than the wild type strain. Mice previously colonised with these strains were protected against septicaemic pneumonia but, unlike mice colonised with the wild type strain, not against <i<S. pneumoniae</i< recolonisation. Conclusions: Colonisation with the <i<∆cps/piaA</i< and <i<∆cps/proABC</i< strains prevented subsequent septicaemia, but in contrast, to published data for encapsulated double mutant strains they did not prevent recolonisation with <i<S. pneumoniae</i<. 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author	Elisa Ramos-Sevillano
spellingShingle	Elisa Ramos-Sevillano misc <i<Streptococcus pneumoniae</i< misc capsule misc vaccine misc <i<psaA</i< misc <i<proABC</i< misc colonisation misc Medicine misc R Protective Effect of Nasal Colonisation with <i<∆cps/piaA</i< and <i<∆cps/proABC</i<<i<Streptococcus pneumoniae</i< Strains against Recolonisation and Invasive Infection
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topic_title	Protective Effect of Nasal Colonisation with <i<∆cps/piaA</i< and <i<∆cps/proABC</i<<i<Streptococcus pneumoniae</i< Strains against Recolonisation and Invasive Infection <i<Streptococcus pneumoniae</i< capsule vaccine <i<psaA</i< <i<proABC</i< colonisation
topic	misc <i<Streptococcus pneumoniae</i< misc capsule misc vaccine misc <i<psaA</i< misc <i<proABC</i< misc colonisation misc Medicine misc R
topic_unstemmed	misc <i<Streptococcus pneumoniae</i< misc capsule misc vaccine misc <i<psaA</i< misc <i<proABC</i< misc colonisation misc Medicine misc R
topic_browse	misc <i<Streptococcus pneumoniae</i< misc capsule misc vaccine misc <i<psaA</i< misc <i<proABC</i< misc colonisation misc Medicine misc R
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title	Protective Effect of Nasal Colonisation with <i<∆cps/piaA</i< and <i<∆cps/proABC</i<<i<Streptococcus pneumoniae</i< Strains against Recolonisation and Invasive Infection
ctrlnum	(DE-627)DOAJ079235166 (DE-599)DOAJ49a7fad3616a46729a4e6aada6193bc7
title_full	Protective Effect of Nasal Colonisation with <i<∆cps/piaA</i< and <i<∆cps/proABC</i<<i<Streptococcus pneumoniae</i< Strains against Recolonisation and Invasive Infection
author_sort	Elisa Ramos-Sevillano
journal	Vaccines
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author_browse	Elisa Ramos-Sevillano Giuseppe Ercoli José Afonso Guerra-Assunção Philip Felgner Rafael Ramiro de Assis Rie Nakajima David Goldblatt Kevin Kweku Adjei Tetteh Robert Simon Heyderman Stephen Brian Gordon Daniela Mulari Ferreria Jeremy Stuart Brown
container_volume	9
format_se	Elektronische Aufsätze
author-letter	Elisa Ramos-Sevillano
doi_str_mv	10.3390/vaccines9030261
author2-role	verfasserin
title_sort	protective effect of nasal colonisation with <i<∆cps/piaa</i< and <i<∆cps/proabc</i<<i<streptococcus pneumoniae</i< strains against recolonisation and invasive infection
title_auth	Protective Effect of Nasal Colonisation with <i<∆cps/piaA</i< and <i<∆cps/proABC</i<<i<Streptococcus pneumoniae</i< Strains against Recolonisation and Invasive Infection
abstract	Rationale: Nasopharyngeal administration of live virulence-attenuated <i<Streptococcus pneumoniae</i< strains is a potential novel preventative strategy. One target for creating reduced virulence <i<S. pneumoniae</i< strains is the capsule, but loss of the capsule reduces the duration of <i<S. pneumoniae</i< colonisation in mice which could impair protective efficacy against subsequent infection. Objectives: To assess protective efficacy of nasopharyngeal administration of unencapsulated <i<S. pneumoniae</i< strains in murine infection models. Methods: Strains containing <i<cps</i< locus deletions combined with the <i<S. pneumoniae</i< virulence factors <i<psaA</i< (reduces colonisation) or <i<proABC</i< (no effect on colonisation) were constructed and their virulence phenotypes and ability to prevent recolonisation or invasive infection assessed using mouse infection models. Serological responses to colonisation were compared between strains using ELISAs, immunoblots and 254 <i<S. pneumoniae</i< protein antigen array. Measurements and Main Results: The <i<∆cps/piaA</i< and <i<∆cps/proABC</i< strains were strongly attenuated in virulence in both invasive infection models and had a reduced ability to colonise the nasopharynx. ELISAs, immunoblots and protein arrays showed colonisation with either strain stimulated weaker serological responses than the wild type strain. Mice previously colonised with these strains were protected against septicaemic pneumonia but, unlike mice colonised with the wild type strain, not against <i<S. pneumoniae</i< recolonisation. Conclusions: Colonisation with the <i<∆cps/piaA</i< and <i<∆cps/proABC</i< strains prevented subsequent septicaemia, but in contrast, to published data for encapsulated double mutant strains they did not prevent recolonisation with <i<S. pneumoniae</i<. These data suggest targeting the <i<cps</i< locus is a less effective option for creating live attenuated strains that prevent <i<S. pneumoniae</i< infections.
abstractGer	Rationale: Nasopharyngeal administration of live virulence-attenuated <i<Streptococcus pneumoniae</i< strains is a potential novel preventative strategy. One target for creating reduced virulence <i<S. pneumoniae</i< strains is the capsule, but loss of the capsule reduces the duration of <i<S. pneumoniae</i< colonisation in mice which could impair protective efficacy against subsequent infection. Objectives: To assess protective efficacy of nasopharyngeal administration of unencapsulated <i<S. pneumoniae</i< strains in murine infection models. Methods: Strains containing <i<cps</i< locus deletions combined with the <i<S. pneumoniae</i< virulence factors <i<psaA</i< (reduces colonisation) or <i<proABC</i< (no effect on colonisation) were constructed and their virulence phenotypes and ability to prevent recolonisation or invasive infection assessed using mouse infection models. Serological responses to colonisation were compared between strains using ELISAs, immunoblots and 254 <i<S. pneumoniae</i< protein antigen array. Measurements and Main Results: The <i<∆cps/piaA</i< and <i<∆cps/proABC</i< strains were strongly attenuated in virulence in both invasive infection models and had a reduced ability to colonise the nasopharynx. ELISAs, immunoblots and protein arrays showed colonisation with either strain stimulated weaker serological responses than the wild type strain. Mice previously colonised with these strains were protected against septicaemic pneumonia but, unlike mice colonised with the wild type strain, not against <i<S. pneumoniae</i< recolonisation. Conclusions: Colonisation with the <i<∆cps/piaA</i< and <i<∆cps/proABC</i< strains prevented subsequent septicaemia, but in contrast, to published data for encapsulated double mutant strains they did not prevent recolonisation with <i<S. pneumoniae</i<. These data suggest targeting the <i<cps</i< locus is a less effective option for creating live attenuated strains that prevent <i<S. pneumoniae</i< infections.
abstract_unstemmed	Rationale: Nasopharyngeal administration of live virulence-attenuated <i<Streptococcus pneumoniae</i< strains is a potential novel preventative strategy. One target for creating reduced virulence <i<S. pneumoniae</i< strains is the capsule, but loss of the capsule reduces the duration of <i<S. pneumoniae</i< colonisation in mice which could impair protective efficacy against subsequent infection. Objectives: To assess protective efficacy of nasopharyngeal administration of unencapsulated <i<S. pneumoniae</i< strains in murine infection models. Methods: Strains containing <i<cps</i< locus deletions combined with the <i<S. pneumoniae</i< virulence factors <i<psaA</i< (reduces colonisation) or <i<proABC</i< (no effect on colonisation) were constructed and their virulence phenotypes and ability to prevent recolonisation or invasive infection assessed using mouse infection models. Serological responses to colonisation were compared between strains using ELISAs, immunoblots and 254 <i<S. pneumoniae</i< protein antigen array. Measurements and Main Results: The <i<∆cps/piaA</i< and <i<∆cps/proABC</i< strains were strongly attenuated in virulence in both invasive infection models and had a reduced ability to colonise the nasopharynx. ELISAs, immunoblots and protein arrays showed colonisation with either strain stimulated weaker serological responses than the wild type strain. Mice previously colonised with these strains were protected against septicaemic pneumonia but, unlike mice colonised with the wild type strain, not against <i<S. pneumoniae</i< recolonisation. Conclusions: Colonisation with the <i<∆cps/piaA</i< and <i<∆cps/proABC</i< strains prevented subsequent septicaemia, but in contrast, to published data for encapsulated double mutant strains they did not prevent recolonisation with <i<S. pneumoniae</i<. These data suggest targeting the <i<cps</i< locus is a less effective option for creating live attenuated strains that prevent <i<S. pneumoniae</i< infections.
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container_issue	3, p 261
title_short	Protective Effect of Nasal Colonisation with <i<∆cps/piaA</i< and <i<∆cps/proABC</i<<i<Streptococcus pneumoniae</i< Strains against Recolonisation and Invasive Infection
url	https://doi.org/10.3390/vaccines9030261 https://doaj.org/article/49a7fad3616a46729a4e6aada6193bc7 https://www.mdpi.com/2076-393X/9/3/261 https://doaj.org/toc/2076-393X
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author2	Giuseppe Ercoli José Afonso Guerra-Assunção Philip Felgner Rafael Ramiro de Assis Rie Nakajima David Goldblatt Kevin Kweku Adjei Tetteh Robert Simon Heyderman Stephen Brian Gordon Daniela Mulari Ferreria Jeremy Stuart Brown
author2Str	Giuseppe Ercoli José Afonso Guerra-Assunção Philip Felgner Rafael Ramiro de Assis Rie Nakajima David Goldblatt Kevin Kweku Adjei Tetteh Robert Simon Heyderman Stephen Brian Gordon Daniela Mulari Ferreria Jeremy Stuart Brown
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doi_str	10.3390/vaccines9030261
up_date	2024-07-03T22:25:31.239Z
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Protective Effect of Nasal Colonisation with <i<∆cps/piaA</i< and <i<∆cps/proABC</i<<i<Streptococcus pneumoniae</i< Strains against Recolonisation and Invasive Infection

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