Screening of Porcine Innate Immune Adaptor Signaling Revealed Several Anti-PRRSV Signaling Pathways

Porcine reproductive and respiratory syndrome virus (PRRSV) causes PRRS and is known to effectively suppress host innate immunity. The current strategies for controlling PRRSV are limited and complete understanding of anti-PRRSV innate immunity is needed. Here, we utilized nine porcine innate immune...
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Autor*in:	Yulin Xu [verfasserIn] Mengxue Ye [verfasserIn] Youwen Zhang [verfasserIn] Shaohua Sun [verfasserIn] Jia Luo [verfasserIn] Sen Jiang [verfasserIn] Jiajia Zhang [verfasserIn] Xueliang Liu [verfasserIn] Qi Shao [verfasserIn] Qi Cao [verfasserIn] Wanglong Zheng [verfasserIn] François Meurens [verfasserIn] Nanhua Chen [verfasserIn] Jianzhong Zhu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	PRRSV innate immunity pattern recognition receptor (PRR) signaling adaptors ectopic expression agonist

Übergeordnetes Werk:	In: Vaccines - MDPI AG, 2013, 9(2021), 10, p 1176
Übergeordnetes Werk:	volume:9 ; year:2021 ; number:10, p 1176

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/vaccines9101176

Katalog-ID:	DOAJ079341756

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520			\|a Porcine reproductive and respiratory syndrome virus (PRRSV) causes PRRS and is known to effectively suppress host innate immunity. The current strategies for controlling PRRSV are limited and complete understanding of anti-PRRSV innate immunity is needed. Here, we utilized nine porcine innate immune signaling adaptors which represent all currently known innate immune receptor signaling pathways for screening of anti-PRRSV activity. The analysis of PRRSV N gene transcription and protein expression both suggested that the multiple ectopic adaptors exhibited varying degrees of anti-PRRSV activities, with TRIF and MAVS most effective. To better quantify the PRRSV replication, the GFP signal of PRRSV from reverse genetics were measured by flow cytometry and similarly varying anti-PRRSV activities by different signaling adaptors were observed. Based on the screening data, and considering the importance of viral nucleic acid in innate immune response, endogenous TRIF, MAVS and STING were selected for further examination of anti-PRRSV activity. Agonist stimulation assay showed that MAVS and STING signaling possessed significant anti-PRRSV activities, whereas siRNA knockdown assay showed that TRIF, MAVS and STING are all involved in anti-PRRSV activity, with TLR3-TRIF displaying discrepancy in anti-PRRSV infection. Nevertheless, our work suggests that multiple pattern recognition receptor (PRR) signaling pathways are involved in anti-PRRSV innate immunity, which may have implications for the development of future antiviral strategies.
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spelling	10.3390/vaccines9101176 doi (DE-627)DOAJ079341756 (DE-599)DOAJ7e3dbf53aad04c6f8a219a3e9270bae2 DE-627 ger DE-627 rakwb eng Yulin Xu verfasserin aut Screening of Porcine Innate Immune Adaptor Signaling Revealed Several Anti-PRRSV Signaling Pathways 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Porcine reproductive and respiratory syndrome virus (PRRSV) causes PRRS and is known to effectively suppress host innate immunity. The current strategies for controlling PRRSV are limited and complete understanding of anti-PRRSV innate immunity is needed. Here, we utilized nine porcine innate immune signaling adaptors which represent all currently known innate immune receptor signaling pathways for screening of anti-PRRSV activity. The analysis of PRRSV N gene transcription and protein expression both suggested that the multiple ectopic adaptors exhibited varying degrees of anti-PRRSV activities, with TRIF and MAVS most effective. To better quantify the PRRSV replication, the GFP signal of PRRSV from reverse genetics were measured by flow cytometry and similarly varying anti-PRRSV activities by different signaling adaptors were observed. Based on the screening data, and considering the importance of viral nucleic acid in innate immune response, endogenous TRIF, MAVS and STING were selected for further examination of anti-PRRSV activity. Agonist stimulation assay showed that MAVS and STING signaling possessed significant anti-PRRSV activities, whereas siRNA knockdown assay showed that TRIF, MAVS and STING are all involved in anti-PRRSV activity, with TLR3-TRIF displaying discrepancy in anti-PRRSV infection. Nevertheless, our work suggests that multiple pattern recognition receptor (PRR) signaling pathways are involved in anti-PRRSV innate immunity, which may have implications for the development of future antiviral strategies. PRRSV innate immunity pattern recognition receptor (PRR) signaling adaptors ectopic expression agonist Medicine R Mengxue Ye verfasserin aut Youwen Zhang verfasserin aut Shaohua Sun verfasserin aut Jia Luo verfasserin aut Sen Jiang verfasserin aut Jiajia Zhang verfasserin aut Xueliang Liu verfasserin aut Qi Shao verfasserin aut Qi Cao verfasserin aut Wanglong Zheng verfasserin aut François Meurens verfasserin aut Nanhua Chen verfasserin aut Jianzhong Zhu verfasserin aut In Vaccines MDPI AG, 2013 9(2021), 10, p 1176 (DE-627)736559205 (DE-600)2703319-3 2076393X nnns volume:9 year:2021 number:10, p 1176 https://doi.org/10.3390/vaccines9101176 kostenfrei https://doaj.org/article/7e3dbf53aad04c6f8a219a3e9270bae2 kostenfrei https://www.mdpi.com/2076-393X/9/10/1176 kostenfrei https://doaj.org/toc/2076-393X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 10, p 1176
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allfieldsSound	10.3390/vaccines9101176 doi (DE-627)DOAJ079341756 (DE-599)DOAJ7e3dbf53aad04c6f8a219a3e9270bae2 DE-627 ger DE-627 rakwb eng Yulin Xu verfasserin aut Screening of Porcine Innate Immune Adaptor Signaling Revealed Several Anti-PRRSV Signaling Pathways 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Porcine reproductive and respiratory syndrome virus (PRRSV) causes PRRS and is known to effectively suppress host innate immunity. The current strategies for controlling PRRSV are limited and complete understanding of anti-PRRSV innate immunity is needed. Here, we utilized nine porcine innate immune signaling adaptors which represent all currently known innate immune receptor signaling pathways for screening of anti-PRRSV activity. The analysis of PRRSV N gene transcription and protein expression both suggested that the multiple ectopic adaptors exhibited varying degrees of anti-PRRSV activities, with TRIF and MAVS most effective. To better quantify the PRRSV replication, the GFP signal of PRRSV from reverse genetics were measured by flow cytometry and similarly varying anti-PRRSV activities by different signaling adaptors were observed. Based on the screening data, and considering the importance of viral nucleic acid in innate immune response, endogenous TRIF, MAVS and STING were selected for further examination of anti-PRRSV activity. Agonist stimulation assay showed that MAVS and STING signaling possessed significant anti-PRRSV activities, whereas siRNA knockdown assay showed that TRIF, MAVS and STING are all involved in anti-PRRSV activity, with TLR3-TRIF displaying discrepancy in anti-PRRSV infection. Nevertheless, our work suggests that multiple pattern recognition receptor (PRR) signaling pathways are involved in anti-PRRSV innate immunity, which may have implications for the development of future antiviral strategies. PRRSV innate immunity pattern recognition receptor (PRR) signaling adaptors ectopic expression agonist Medicine R Mengxue Ye verfasserin aut Youwen Zhang verfasserin aut Shaohua Sun verfasserin aut Jia Luo verfasserin aut Sen Jiang verfasserin aut Jiajia Zhang verfasserin aut Xueliang Liu verfasserin aut Qi Shao verfasserin aut Qi Cao verfasserin aut Wanglong Zheng verfasserin aut François Meurens verfasserin aut Nanhua Chen verfasserin aut Jianzhong Zhu verfasserin aut In Vaccines MDPI AG, 2013 9(2021), 10, p 1176 (DE-627)736559205 (DE-600)2703319-3 2076393X nnns volume:9 year:2021 number:10, p 1176 https://doi.org/10.3390/vaccines9101176 kostenfrei https://doaj.org/article/7e3dbf53aad04c6f8a219a3e9270bae2 kostenfrei https://www.mdpi.com/2076-393X/9/10/1176 kostenfrei https://doaj.org/toc/2076-393X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 10, p 1176
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source	In Vaccines 9(2021), 10, p 1176 volume:9 year:2021 number:10, p 1176
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authorswithroles_txt_mv	Yulin Xu @@aut@@ Mengxue Ye @@aut@@ Youwen Zhang @@aut@@ Shaohua Sun @@aut@@ Jia Luo @@aut@@ Sen Jiang @@aut@@ Jiajia Zhang @@aut@@ Xueliang Liu @@aut@@ Qi Shao @@aut@@ Qi Cao @@aut@@ Wanglong Zheng @@aut@@ François Meurens @@aut@@ Nanhua Chen @@aut@@ Jianzhong Zhu @@aut@@
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author	Yulin Xu
spellingShingle	Yulin Xu misc PRRSV misc innate immunity misc pattern recognition receptor (PRR) misc signaling adaptors misc ectopic expression misc agonist misc Medicine misc R Screening of Porcine Innate Immune Adaptor Signaling Revealed Several Anti-PRRSV Signaling Pathways
authorStr	Yulin Xu
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topic_title	Screening of Porcine Innate Immune Adaptor Signaling Revealed Several Anti-PRRSV Signaling Pathways PRRSV innate immunity pattern recognition receptor (PRR) signaling adaptors ectopic expression agonist
topic	misc PRRSV misc innate immunity misc pattern recognition receptor (PRR) misc signaling adaptors misc ectopic expression misc agonist misc Medicine misc R
topic_unstemmed	misc PRRSV misc innate immunity misc pattern recognition receptor (PRR) misc signaling adaptors misc ectopic expression misc agonist misc Medicine misc R
topic_browse	misc PRRSV misc innate immunity misc pattern recognition receptor (PRR) misc signaling adaptors misc ectopic expression misc agonist misc Medicine misc R
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title	Screening of Porcine Innate Immune Adaptor Signaling Revealed Several Anti-PRRSV Signaling Pathways
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title_full	Screening of Porcine Innate Immune Adaptor Signaling Revealed Several Anti-PRRSV Signaling Pathways
author_sort	Yulin Xu
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author_browse	Yulin Xu Mengxue Ye Youwen Zhang Shaohua Sun Jia Luo Sen Jiang Jiajia Zhang Xueliang Liu Qi Shao Qi Cao Wanglong Zheng François Meurens Nanhua Chen Jianzhong Zhu
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title_sort	screening of porcine innate immune adaptor signaling revealed several anti-prrsv signaling pathways
title_auth	Screening of Porcine Innate Immune Adaptor Signaling Revealed Several Anti-PRRSV Signaling Pathways
abstract	Porcine reproductive and respiratory syndrome virus (PRRSV) causes PRRS and is known to effectively suppress host innate immunity. The current strategies for controlling PRRSV are limited and complete understanding of anti-PRRSV innate immunity is needed. Here, we utilized nine porcine innate immune signaling adaptors which represent all currently known innate immune receptor signaling pathways for screening of anti-PRRSV activity. The analysis of PRRSV N gene transcription and protein expression both suggested that the multiple ectopic adaptors exhibited varying degrees of anti-PRRSV activities, with TRIF and MAVS most effective. To better quantify the PRRSV replication, the GFP signal of PRRSV from reverse genetics were measured by flow cytometry and similarly varying anti-PRRSV activities by different signaling adaptors were observed. Based on the screening data, and considering the importance of viral nucleic acid in innate immune response, endogenous TRIF, MAVS and STING were selected for further examination of anti-PRRSV activity. Agonist stimulation assay showed that MAVS and STING signaling possessed significant anti-PRRSV activities, whereas siRNA knockdown assay showed that TRIF, MAVS and STING are all involved in anti-PRRSV activity, with TLR3-TRIF displaying discrepancy in anti-PRRSV infection. Nevertheless, our work suggests that multiple pattern recognition receptor (PRR) signaling pathways are involved in anti-PRRSV innate immunity, which may have implications for the development of future antiviral strategies.
abstractGer	Porcine reproductive and respiratory syndrome virus (PRRSV) causes PRRS and is known to effectively suppress host innate immunity. The current strategies for controlling PRRSV are limited and complete understanding of anti-PRRSV innate immunity is needed. Here, we utilized nine porcine innate immune signaling adaptors which represent all currently known innate immune receptor signaling pathways for screening of anti-PRRSV activity. The analysis of PRRSV N gene transcription and protein expression both suggested that the multiple ectopic adaptors exhibited varying degrees of anti-PRRSV activities, with TRIF and MAVS most effective. To better quantify the PRRSV replication, the GFP signal of PRRSV from reverse genetics were measured by flow cytometry and similarly varying anti-PRRSV activities by different signaling adaptors were observed. Based on the screening data, and considering the importance of viral nucleic acid in innate immune response, endogenous TRIF, MAVS and STING were selected for further examination of anti-PRRSV activity. Agonist stimulation assay showed that MAVS and STING signaling possessed significant anti-PRRSV activities, whereas siRNA knockdown assay showed that TRIF, MAVS and STING are all involved in anti-PRRSV activity, with TLR3-TRIF displaying discrepancy in anti-PRRSV infection. Nevertheless, our work suggests that multiple pattern recognition receptor (PRR) signaling pathways are involved in anti-PRRSV innate immunity, which may have implications for the development of future antiviral strategies.
abstract_unstemmed	Porcine reproductive and respiratory syndrome virus (PRRSV) causes PRRS and is known to effectively suppress host innate immunity. The current strategies for controlling PRRSV are limited and complete understanding of anti-PRRSV innate immunity is needed. Here, we utilized nine porcine innate immune signaling adaptors which represent all currently known innate immune receptor signaling pathways for screening of anti-PRRSV activity. The analysis of PRRSV N gene transcription and protein expression both suggested that the multiple ectopic adaptors exhibited varying degrees of anti-PRRSV activities, with TRIF and MAVS most effective. To better quantify the PRRSV replication, the GFP signal of PRRSV from reverse genetics were measured by flow cytometry and similarly varying anti-PRRSV activities by different signaling adaptors were observed. Based on the screening data, and considering the importance of viral nucleic acid in innate immune response, endogenous TRIF, MAVS and STING were selected for further examination of anti-PRRSV activity. Agonist stimulation assay showed that MAVS and STING signaling possessed significant anti-PRRSV activities, whereas siRNA knockdown assay showed that TRIF, MAVS and STING are all involved in anti-PRRSV activity, with TLR3-TRIF displaying discrepancy in anti-PRRSV infection. Nevertheless, our work suggests that multiple pattern recognition receptor (PRR) signaling pathways are involved in anti-PRRSV innate immunity, which may have implications for the development of future antiviral strategies.
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container_issue	10, p 1176
title_short	Screening of Porcine Innate Immune Adaptor Signaling Revealed Several Anti-PRRSV Signaling Pathways
url	https://doi.org/10.3390/vaccines9101176 https://doaj.org/article/7e3dbf53aad04c6f8a219a3e9270bae2 https://www.mdpi.com/2076-393X/9/10/1176 https://doaj.org/toc/2076-393X
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author2	Mengxue Ye Youwen Zhang Shaohua Sun Jia Luo Sen Jiang Jiajia Zhang Xueliang Liu Qi Shao Qi Cao Wanglong Zheng François Meurens Nanhua Chen Jianzhong Zhu
author2Str	Mengxue Ye Youwen Zhang Shaohua Sun Jia Luo Sen Jiang Jiajia Zhang Xueliang Liu Qi Shao Qi Cao Wanglong Zheng François Meurens Nanhua Chen Jianzhong Zhu
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doi_str	10.3390/vaccines9101176
up_date	2024-07-03T22:59:23.266Z
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Agonist stimulation assay showed that MAVS and STING signaling possessed significant anti-PRRSV activities, whereas siRNA knockdown assay showed that TRIF, MAVS and STING are all involved in anti-PRRSV activity, with TLR3-TRIF displaying discrepancy in anti-PRRSV infection. 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Screening of Porcine Innate Immune Adaptor Signaling Revealed Several Anti-PRRSV Signaling Pathways

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