Screening of Porcine Innate Immune Adaptor Signaling Revealed Several Anti-PRRSV Signaling Pathways
Porcine reproductive and respiratory syndrome virus (PRRSV) causes PRRS and is known to effectively suppress host innate immunity. The current strategies for controlling PRRSV are limited and complete understanding of anti-PRRSV innate immunity is needed. Here, we utilized nine porcine innate immune...
Ausführliche Beschreibung
Autor*in: |
Yulin Xu [verfasserIn] Mengxue Ye [verfasserIn] Youwen Zhang [verfasserIn] Shaohua Sun [verfasserIn] Jia Luo [verfasserIn] Sen Jiang [verfasserIn] Jiajia Zhang [verfasserIn] Xueliang Liu [verfasserIn] Qi Shao [verfasserIn] Qi Cao [verfasserIn] Wanglong Zheng [verfasserIn] François Meurens [verfasserIn] Nanhua Chen [verfasserIn] Jianzhong Zhu [verfasserIn] |
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Format: |
E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2021 |
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Schlagwörter: |
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Übergeordnetes Werk: |
In: Vaccines - MDPI AG, 2013, 9(2021), 10, p 1176 |
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Übergeordnetes Werk: |
volume:9 ; year:2021 ; number:10, p 1176 |
Links: |
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DOI / URN: |
10.3390/vaccines9101176 |
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Katalog-ID: |
DOAJ079341756 |
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10.3390/vaccines9101176 doi (DE-627)DOAJ079341756 (DE-599)DOAJ7e3dbf53aad04c6f8a219a3e9270bae2 DE-627 ger DE-627 rakwb eng Yulin Xu verfasserin aut Screening of Porcine Innate Immune Adaptor Signaling Revealed Several Anti-PRRSV Signaling Pathways 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Porcine reproductive and respiratory syndrome virus (PRRSV) causes PRRS and is known to effectively suppress host innate immunity. The current strategies for controlling PRRSV are limited and complete understanding of anti-PRRSV innate immunity is needed. Here, we utilized nine porcine innate immune signaling adaptors which represent all currently known innate immune receptor signaling pathways for screening of anti-PRRSV activity. The analysis of PRRSV N gene transcription and protein expression both suggested that the multiple ectopic adaptors exhibited varying degrees of anti-PRRSV activities, with TRIF and MAVS most effective. To better quantify the PRRSV replication, the GFP signal of PRRSV from reverse genetics were measured by flow cytometry and similarly varying anti-PRRSV activities by different signaling adaptors were observed. Based on the screening data, and considering the importance of viral nucleic acid in innate immune response, endogenous TRIF, MAVS and STING were selected for further examination of anti-PRRSV activity. Agonist stimulation assay showed that MAVS and STING signaling possessed significant anti-PRRSV activities, whereas siRNA knockdown assay showed that TRIF, MAVS and STING are all involved in anti-PRRSV activity, with TLR3-TRIF displaying discrepancy in anti-PRRSV infection. Nevertheless, our work suggests that multiple pattern recognition receptor (PRR) signaling pathways are involved in anti-PRRSV innate immunity, which may have implications for the development of future antiviral strategies. PRRSV innate immunity pattern recognition receptor (PRR) signaling adaptors ectopic expression agonist Medicine R Mengxue Ye verfasserin aut Youwen Zhang verfasserin aut Shaohua Sun verfasserin aut Jia Luo verfasserin aut Sen Jiang verfasserin aut Jiajia Zhang verfasserin aut Xueliang Liu verfasserin aut Qi Shao verfasserin aut Qi Cao verfasserin aut Wanglong Zheng verfasserin aut François Meurens verfasserin aut Nanhua Chen verfasserin aut Jianzhong Zhu verfasserin aut In Vaccines MDPI AG, 2013 9(2021), 10, p 1176 (DE-627)736559205 (DE-600)2703319-3 2076393X nnns volume:9 year:2021 number:10, p 1176 https://doi.org/10.3390/vaccines9101176 kostenfrei https://doaj.org/article/7e3dbf53aad04c6f8a219a3e9270bae2 kostenfrei https://www.mdpi.com/2076-393X/9/10/1176 kostenfrei https://doaj.org/toc/2076-393X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 10, p 1176 |
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10.3390/vaccines9101176 doi (DE-627)DOAJ079341756 (DE-599)DOAJ7e3dbf53aad04c6f8a219a3e9270bae2 DE-627 ger DE-627 rakwb eng Yulin Xu verfasserin aut Screening of Porcine Innate Immune Adaptor Signaling Revealed Several Anti-PRRSV Signaling Pathways 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Porcine reproductive and respiratory syndrome virus (PRRSV) causes PRRS and is known to effectively suppress host innate immunity. The current strategies for controlling PRRSV are limited and complete understanding of anti-PRRSV innate immunity is needed. Here, we utilized nine porcine innate immune signaling adaptors which represent all currently known innate immune receptor signaling pathways for screening of anti-PRRSV activity. The analysis of PRRSV N gene transcription and protein expression both suggested that the multiple ectopic adaptors exhibited varying degrees of anti-PRRSV activities, with TRIF and MAVS most effective. To better quantify the PRRSV replication, the GFP signal of PRRSV from reverse genetics were measured by flow cytometry and similarly varying anti-PRRSV activities by different signaling adaptors were observed. Based on the screening data, and considering the importance of viral nucleic acid in innate immune response, endogenous TRIF, MAVS and STING were selected for further examination of anti-PRRSV activity. Agonist stimulation assay showed that MAVS and STING signaling possessed significant anti-PRRSV activities, whereas siRNA knockdown assay showed that TRIF, MAVS and STING are all involved in anti-PRRSV activity, with TLR3-TRIF displaying discrepancy in anti-PRRSV infection. Nevertheless, our work suggests that multiple pattern recognition receptor (PRR) signaling pathways are involved in anti-PRRSV innate immunity, which may have implications for the development of future antiviral strategies. PRRSV innate immunity pattern recognition receptor (PRR) signaling adaptors ectopic expression agonist Medicine R Mengxue Ye verfasserin aut Youwen Zhang verfasserin aut Shaohua Sun verfasserin aut Jia Luo verfasserin aut Sen Jiang verfasserin aut Jiajia Zhang verfasserin aut Xueliang Liu verfasserin aut Qi Shao verfasserin aut Qi Cao verfasserin aut Wanglong Zheng verfasserin aut François Meurens verfasserin aut Nanhua Chen verfasserin aut Jianzhong Zhu verfasserin aut In Vaccines MDPI AG, 2013 9(2021), 10, p 1176 (DE-627)736559205 (DE-600)2703319-3 2076393X nnns volume:9 year:2021 number:10, p 1176 https://doi.org/10.3390/vaccines9101176 kostenfrei https://doaj.org/article/7e3dbf53aad04c6f8a219a3e9270bae2 kostenfrei https://www.mdpi.com/2076-393X/9/10/1176 kostenfrei https://doaj.org/toc/2076-393X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 10, p 1176 |
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10.3390/vaccines9101176 doi (DE-627)DOAJ079341756 (DE-599)DOAJ7e3dbf53aad04c6f8a219a3e9270bae2 DE-627 ger DE-627 rakwb eng Yulin Xu verfasserin aut Screening of Porcine Innate Immune Adaptor Signaling Revealed Several Anti-PRRSV Signaling Pathways 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Porcine reproductive and respiratory syndrome virus (PRRSV) causes PRRS and is known to effectively suppress host innate immunity. The current strategies for controlling PRRSV are limited and complete understanding of anti-PRRSV innate immunity is needed. Here, we utilized nine porcine innate immune signaling adaptors which represent all currently known innate immune receptor signaling pathways for screening of anti-PRRSV activity. The analysis of PRRSV N gene transcription and protein expression both suggested that the multiple ectopic adaptors exhibited varying degrees of anti-PRRSV activities, with TRIF and MAVS most effective. To better quantify the PRRSV replication, the GFP signal of PRRSV from reverse genetics were measured by flow cytometry and similarly varying anti-PRRSV activities by different signaling adaptors were observed. Based on the screening data, and considering the importance of viral nucleic acid in innate immune response, endogenous TRIF, MAVS and STING were selected for further examination of anti-PRRSV activity. Agonist stimulation assay showed that MAVS and STING signaling possessed significant anti-PRRSV activities, whereas siRNA knockdown assay showed that TRIF, MAVS and STING are all involved in anti-PRRSV activity, with TLR3-TRIF displaying discrepancy in anti-PRRSV infection. Nevertheless, our work suggests that multiple pattern recognition receptor (PRR) signaling pathways are involved in anti-PRRSV innate immunity, which may have implications for the development of future antiviral strategies. PRRSV innate immunity pattern recognition receptor (PRR) signaling adaptors ectopic expression agonist Medicine R Mengxue Ye verfasserin aut Youwen Zhang verfasserin aut Shaohua Sun verfasserin aut Jia Luo verfasserin aut Sen Jiang verfasserin aut Jiajia Zhang verfasserin aut Xueliang Liu verfasserin aut Qi Shao verfasserin aut Qi Cao verfasserin aut Wanglong Zheng verfasserin aut François Meurens verfasserin aut Nanhua Chen verfasserin aut Jianzhong Zhu verfasserin aut In Vaccines MDPI AG, 2013 9(2021), 10, p 1176 (DE-627)736559205 (DE-600)2703319-3 2076393X nnns volume:9 year:2021 number:10, p 1176 https://doi.org/10.3390/vaccines9101176 kostenfrei https://doaj.org/article/7e3dbf53aad04c6f8a219a3e9270bae2 kostenfrei https://www.mdpi.com/2076-393X/9/10/1176 kostenfrei https://doaj.org/toc/2076-393X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 10, p 1176 |
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10.3390/vaccines9101176 doi (DE-627)DOAJ079341756 (DE-599)DOAJ7e3dbf53aad04c6f8a219a3e9270bae2 DE-627 ger DE-627 rakwb eng Yulin Xu verfasserin aut Screening of Porcine Innate Immune Adaptor Signaling Revealed Several Anti-PRRSV Signaling Pathways 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Porcine reproductive and respiratory syndrome virus (PRRSV) causes PRRS and is known to effectively suppress host innate immunity. The current strategies for controlling PRRSV are limited and complete understanding of anti-PRRSV innate immunity is needed. Here, we utilized nine porcine innate immune signaling adaptors which represent all currently known innate immune receptor signaling pathways for screening of anti-PRRSV activity. The analysis of PRRSV N gene transcription and protein expression both suggested that the multiple ectopic adaptors exhibited varying degrees of anti-PRRSV activities, with TRIF and MAVS most effective. To better quantify the PRRSV replication, the GFP signal of PRRSV from reverse genetics were measured by flow cytometry and similarly varying anti-PRRSV activities by different signaling adaptors were observed. Based on the screening data, and considering the importance of viral nucleic acid in innate immune response, endogenous TRIF, MAVS and STING were selected for further examination of anti-PRRSV activity. Agonist stimulation assay showed that MAVS and STING signaling possessed significant anti-PRRSV activities, whereas siRNA knockdown assay showed that TRIF, MAVS and STING are all involved in anti-PRRSV activity, with TLR3-TRIF displaying discrepancy in anti-PRRSV infection. Nevertheless, our work suggests that multiple pattern recognition receptor (PRR) signaling pathways are involved in anti-PRRSV innate immunity, which may have implications for the development of future antiviral strategies. PRRSV innate immunity pattern recognition receptor (PRR) signaling adaptors ectopic expression agonist Medicine R Mengxue Ye verfasserin aut Youwen Zhang verfasserin aut Shaohua Sun verfasserin aut Jia Luo verfasserin aut Sen Jiang verfasserin aut Jiajia Zhang verfasserin aut Xueliang Liu verfasserin aut Qi Shao verfasserin aut Qi Cao verfasserin aut Wanglong Zheng verfasserin aut François Meurens verfasserin aut Nanhua Chen verfasserin aut Jianzhong Zhu verfasserin aut In Vaccines MDPI AG, 2013 9(2021), 10, p 1176 (DE-627)736559205 (DE-600)2703319-3 2076393X nnns volume:9 year:2021 number:10, p 1176 https://doi.org/10.3390/vaccines9101176 kostenfrei https://doaj.org/article/7e3dbf53aad04c6f8a219a3e9270bae2 kostenfrei https://www.mdpi.com/2076-393X/9/10/1176 kostenfrei https://doaj.org/toc/2076-393X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 10, p 1176 |
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10.3390/vaccines9101176 doi (DE-627)DOAJ079341756 (DE-599)DOAJ7e3dbf53aad04c6f8a219a3e9270bae2 DE-627 ger DE-627 rakwb eng Yulin Xu verfasserin aut Screening of Porcine Innate Immune Adaptor Signaling Revealed Several Anti-PRRSV Signaling Pathways 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Porcine reproductive and respiratory syndrome virus (PRRSV) causes PRRS and is known to effectively suppress host innate immunity. The current strategies for controlling PRRSV are limited and complete understanding of anti-PRRSV innate immunity is needed. Here, we utilized nine porcine innate immune signaling adaptors which represent all currently known innate immune receptor signaling pathways for screening of anti-PRRSV activity. The analysis of PRRSV N gene transcription and protein expression both suggested that the multiple ectopic adaptors exhibited varying degrees of anti-PRRSV activities, with TRIF and MAVS most effective. To better quantify the PRRSV replication, the GFP signal of PRRSV from reverse genetics were measured by flow cytometry and similarly varying anti-PRRSV activities by different signaling adaptors were observed. Based on the screening data, and considering the importance of viral nucleic acid in innate immune response, endogenous TRIF, MAVS and STING were selected for further examination of anti-PRRSV activity. Agonist stimulation assay showed that MAVS and STING signaling possessed significant anti-PRRSV activities, whereas siRNA knockdown assay showed that TRIF, MAVS and STING are all involved in anti-PRRSV activity, with TLR3-TRIF displaying discrepancy in anti-PRRSV infection. Nevertheless, our work suggests that multiple pattern recognition receptor (PRR) signaling pathways are involved in anti-PRRSV innate immunity, which may have implications for the development of future antiviral strategies. PRRSV innate immunity pattern recognition receptor (PRR) signaling adaptors ectopic expression agonist Medicine R Mengxue Ye verfasserin aut Youwen Zhang verfasserin aut Shaohua Sun verfasserin aut Jia Luo verfasserin aut Sen Jiang verfasserin aut Jiajia Zhang verfasserin aut Xueliang Liu verfasserin aut Qi Shao verfasserin aut Qi Cao verfasserin aut Wanglong Zheng verfasserin aut François Meurens verfasserin aut Nanhua Chen verfasserin aut Jianzhong Zhu verfasserin aut In Vaccines MDPI AG, 2013 9(2021), 10, p 1176 (DE-627)736559205 (DE-600)2703319-3 2076393X nnns volume:9 year:2021 number:10, p 1176 https://doi.org/10.3390/vaccines9101176 kostenfrei https://doaj.org/article/7e3dbf53aad04c6f8a219a3e9270bae2 kostenfrei https://www.mdpi.com/2076-393X/9/10/1176 kostenfrei https://doaj.org/toc/2076-393X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 10, p 1176 |
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Yulin Xu @@aut@@ Mengxue Ye @@aut@@ Youwen Zhang @@aut@@ Shaohua Sun @@aut@@ Jia Luo @@aut@@ Sen Jiang @@aut@@ Jiajia Zhang @@aut@@ Xueliang Liu @@aut@@ Qi Shao @@aut@@ Qi Cao @@aut@@ Wanglong Zheng @@aut@@ François Meurens @@aut@@ Nanhua Chen @@aut@@ Jianzhong Zhu @@aut@@ |
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2021-01-01T00:00:00Z |
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Yulin Xu |
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Screening of Porcine Innate Immune Adaptor Signaling Revealed Several Anti-PRRSV Signaling Pathways PRRSV innate immunity pattern recognition receptor (PRR) signaling adaptors ectopic expression agonist |
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screening of porcine innate immune adaptor signaling revealed several anti-prrsv signaling pathways |
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Screening of Porcine Innate Immune Adaptor Signaling Revealed Several Anti-PRRSV Signaling Pathways |
abstract |
Porcine reproductive and respiratory syndrome virus (PRRSV) causes PRRS and is known to effectively suppress host innate immunity. The current strategies for controlling PRRSV are limited and complete understanding of anti-PRRSV innate immunity is needed. Here, we utilized nine porcine innate immune signaling adaptors which represent all currently known innate immune receptor signaling pathways for screening of anti-PRRSV activity. The analysis of PRRSV N gene transcription and protein expression both suggested that the multiple ectopic adaptors exhibited varying degrees of anti-PRRSV activities, with TRIF and MAVS most effective. To better quantify the PRRSV replication, the GFP signal of PRRSV from reverse genetics were measured by flow cytometry and similarly varying anti-PRRSV activities by different signaling adaptors were observed. Based on the screening data, and considering the importance of viral nucleic acid in innate immune response, endogenous TRIF, MAVS and STING were selected for further examination of anti-PRRSV activity. Agonist stimulation assay showed that MAVS and STING signaling possessed significant anti-PRRSV activities, whereas siRNA knockdown assay showed that TRIF, MAVS and STING are all involved in anti-PRRSV activity, with TLR3-TRIF displaying discrepancy in anti-PRRSV infection. Nevertheless, our work suggests that multiple pattern recognition receptor (PRR) signaling pathways are involved in anti-PRRSV innate immunity, which may have implications for the development of future antiviral strategies. |
abstractGer |
Porcine reproductive and respiratory syndrome virus (PRRSV) causes PRRS and is known to effectively suppress host innate immunity. The current strategies for controlling PRRSV are limited and complete understanding of anti-PRRSV innate immunity is needed. Here, we utilized nine porcine innate immune signaling adaptors which represent all currently known innate immune receptor signaling pathways for screening of anti-PRRSV activity. The analysis of PRRSV N gene transcription and protein expression both suggested that the multiple ectopic adaptors exhibited varying degrees of anti-PRRSV activities, with TRIF and MAVS most effective. To better quantify the PRRSV replication, the GFP signal of PRRSV from reverse genetics were measured by flow cytometry and similarly varying anti-PRRSV activities by different signaling adaptors were observed. Based on the screening data, and considering the importance of viral nucleic acid in innate immune response, endogenous TRIF, MAVS and STING were selected for further examination of anti-PRRSV activity. Agonist stimulation assay showed that MAVS and STING signaling possessed significant anti-PRRSV activities, whereas siRNA knockdown assay showed that TRIF, MAVS and STING are all involved in anti-PRRSV activity, with TLR3-TRIF displaying discrepancy in anti-PRRSV infection. Nevertheless, our work suggests that multiple pattern recognition receptor (PRR) signaling pathways are involved in anti-PRRSV innate immunity, which may have implications for the development of future antiviral strategies. |
abstract_unstemmed |
Porcine reproductive and respiratory syndrome virus (PRRSV) causes PRRS and is known to effectively suppress host innate immunity. The current strategies for controlling PRRSV are limited and complete understanding of anti-PRRSV innate immunity is needed. Here, we utilized nine porcine innate immune signaling adaptors which represent all currently known innate immune receptor signaling pathways for screening of anti-PRRSV activity. The analysis of PRRSV N gene transcription and protein expression both suggested that the multiple ectopic adaptors exhibited varying degrees of anti-PRRSV activities, with TRIF and MAVS most effective. To better quantify the PRRSV replication, the GFP signal of PRRSV from reverse genetics were measured by flow cytometry and similarly varying anti-PRRSV activities by different signaling adaptors were observed. Based on the screening data, and considering the importance of viral nucleic acid in innate immune response, endogenous TRIF, MAVS and STING were selected for further examination of anti-PRRSV activity. Agonist stimulation assay showed that MAVS and STING signaling possessed significant anti-PRRSV activities, whereas siRNA knockdown assay showed that TRIF, MAVS and STING are all involved in anti-PRRSV activity, with TLR3-TRIF displaying discrepancy in anti-PRRSV infection. Nevertheless, our work suggests that multiple pattern recognition receptor (PRR) signaling pathways are involved in anti-PRRSV innate immunity, which may have implications for the development of future antiviral strategies. |
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The current strategies for controlling PRRSV are limited and complete understanding of anti-PRRSV innate immunity is needed. Here, we utilized nine porcine innate immune signaling adaptors which represent all currently known innate immune receptor signaling pathways for screening of anti-PRRSV activity. The analysis of PRRSV N gene transcription and protein expression both suggested that the multiple ectopic adaptors exhibited varying degrees of anti-PRRSV activities, with TRIF and MAVS most effective. To better quantify the PRRSV replication, the GFP signal of PRRSV from reverse genetics were measured by flow cytometry and similarly varying anti-PRRSV activities by different signaling adaptors were observed. Based on the screening data, and considering the importance of viral nucleic acid in innate immune response, endogenous TRIF, MAVS and STING were selected for further examination of anti-PRRSV activity. Agonist stimulation assay showed that MAVS and STING signaling possessed significant anti-PRRSV activities, whereas siRNA knockdown assay showed that TRIF, MAVS and STING are all involved in anti-PRRSV activity, with TLR3-TRIF displaying discrepancy in anti-PRRSV infection. Nevertheless, our work suggests that multiple pattern recognition receptor (PRR) signaling pathways are involved in anti-PRRSV innate immunity, which may have implications for the development of future antiviral strategies.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">PRRSV</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">innate immunity</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">pattern recognition receptor (PRR)</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">signaling adaptors</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">ectopic expression</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">agonist</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Medicine</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield 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7.400717 |