Comparison of Serological and Molecular Assays for <i<Bartonella</i< Species in Dogs with Hemangiosarcoma

Currently, a gold standard diagnostic test for <i<Bartonella</i< infection in dogs is lacking. This represents a critical limitation for the development and evaluation of new diagnostic tests, as well as for the diagnosis of, and research on, bartonellosis in dogs. This retrospective observational study aims to compare the results of commonly performed and newly-reported <i<Bartonella</i< spp. diagnostic tests in banked clinical specimens from 90 dogs with hemangiosarcoma (HSA) using composite reference standard (CRS) and random effects latent class analysis (RE-LCA) techniques. Samples from each dog were tested using six serological or molecular diagnostic assays, including indirect fluorescent antibody (IFA) and Western blot (WB) for the detection of antibodies in serum, and qPCR and droplet digital PCR (ddPCR) in blood and fresh frozen tissue biopsy samples (mainly splenic HSA tumors and histopathologically normal spleen or skin/adipose tissue). <i<Bartonella</i< infection prevalence was estimated to be 78% based on the CRS (parallel testing with all six assays), and 64% based on the RE-LCA model. The assay with the highest diagnostic accuracy was qPCR performed on fresh frozen tissue biopsy samples (sensitivity: 94% by RE-LCA and 80% by CRS; specificity: 100%). When comparing newly-reported to traditional <i<Bartonella</i< diagnostic assays, ddPCR was more sensitive for the detection of <i<Bartonella</i< DNA than qPCR when testing blood samples (36% vs. 0%, <i<p</i< < 0.0001). Dogs that were positive on serological assays alone with negative molecular assays were highly unlikely (<3%) to be classified as infected by the RE-LCA model. These data indicate that <i<Bartonella</i< spp. DNA can be PCR amplified from fresh frozen tissues from a majority of dogs with HSA using both qPCR and ddPCR, supporting the use of these methods for future controlled studies comparing the prevalence of <i<Bartonella</i< spp. DNA in the tissue of dogs with HSA to that of unaffected controls. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Erin Lashnits [verfasserIn] Pradeep Neupane [verfasserIn] Julie M. Bradley [verfasserIn] Toni Richardson [verfasserIn] Ricardo G. Maggi [verfasserIn] Edward B. Breitschwerdt [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	diagnostic testing ddPCR PCR serology sensitivity specificity

Übergeordnetes Werk:	In: Pathogens - MDPI AG, 2012, 10(2021), 7, p 794
Übergeordnetes Werk:	volume:10 ; year:2021 ; number:7, p 794

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/pathogens10070794

Katalog-ID:	DOAJ079363393

Internformat


LEADER	01000caa a22002652 4500
001	DOAJ079363393
003	DE-627
005	20240412173508.0
007	cr uuu---uuuuu
008	230307s2021 xx \|\|\|\|\|o 00\| \|\|eng c
024	7		\|a 10.3390/pathogens10070794 \|2 doi
035			\|a (DE-627)DOAJ079363393
035			\|a (DE-599)DOAJ9caaff90501140c997dca552bb963043
040			\|a DE-627 \|b ger \|c DE-627 \|e rakwb
041			\|a eng
100	0		\|a Erin Lashnits \|e verfasserin \|4 aut
245	1	0	\|a Comparison of Serological and Molecular Assays for <i<Bartonella</i< Species in Dogs with Hemangiosarcoma
264		1	\|c 2021
336			\|a Text \|b txt \|2 rdacontent
337			\|a Computermedien \|b c \|2 rdamedia
338			\|a Online-Ressource \|b cr \|2 rdacarrier
520			\|a Currently, a gold standard diagnostic test for <i<Bartonella</i< infection in dogs is lacking. This represents a critical limitation for the development and evaluation of new diagnostic tests, as well as for the diagnosis of, and research on, bartonellosis in dogs. This retrospective observational study aims to compare the results of commonly performed and newly-reported <i<Bartonella</i< spp. diagnostic tests in banked clinical specimens from 90 dogs with hemangiosarcoma (HSA) using composite reference standard (CRS) and random effects latent class analysis (RE-LCA) techniques. Samples from each dog were tested using six serological or molecular diagnostic assays, including indirect fluorescent antibody (IFA) and Western blot (WB) for the detection of antibodies in serum, and qPCR and droplet digital PCR (ddPCR) in blood and fresh frozen tissue biopsy samples (mainly splenic HSA tumors and histopathologically normal spleen or skin/adipose tissue). <i<Bartonella</i< infection prevalence was estimated to be 78% based on the CRS (parallel testing with all six assays), and 64% based on the RE-LCA model. The assay with the highest diagnostic accuracy was qPCR performed on fresh frozen tissue biopsy samples (sensitivity: 94% by RE-LCA and 80% by CRS; specificity: 100%). When comparing newly-reported to traditional <i<Bartonella</i< diagnostic assays, ddPCR was more sensitive for the detection of <i<Bartonella</i< DNA than qPCR when testing blood samples (36% vs. 0%, <i<p</i< < 0.0001). Dogs that were positive on serological assays alone with negative molecular assays were highly unlikely (<3%) to be classified as infected by the RE-LCA model. These data indicate that <i<Bartonella</i< spp. DNA can be PCR amplified from fresh frozen tissues from a majority of dogs with HSA using both qPCR and ddPCR, supporting the use of these methods for future controlled studies comparing the prevalence of <i<Bartonella</i< spp. DNA in the tissue of dogs with HSA to that of unaffected controls.
650		4	\|a diagnostic testing
650		4	\|a ddPCR
650		4	\|a PCR
650		4	\|a serology
650		4	\|a sensitivity
650		4	\|a specificity
653		0	\|a Medicine
653		0	\|a R
700	0		\|a Pradeep Neupane \|e verfasserin \|4 aut
700	0		\|a Julie M. Bradley \|e verfasserin \|4 aut
700	0		\|a Toni Richardson \|e verfasserin \|4 aut
700	0		\|a Ricardo G. Maggi \|e verfasserin \|4 aut
700	0		\|a Edward B. Breitschwerdt \|e verfasserin \|4 aut
773	0	8	\|i In \|t Pathogens \|d MDPI AG, 2012 \|g 10(2021), 7, p 794 \|w (DE-627)732627885 \|w (DE-600)2695572-6 \|x 20760817 \|7 nnns
773	1	8	\|g volume:10 \|g year:2021 \|g number:7, p 794
856	4	0	\|u https://doi.org/10.3390/pathogens10070794 \|z kostenfrei
856	4	0	\|u https://doaj.org/article/9caaff90501140c997dca552bb963043 \|z kostenfrei
856	4	0	\|u https://www.mdpi.com/2076-0817/10/7/794 \|z kostenfrei
856	4	2	\|u https://doaj.org/toc/2076-0817 \|y Journal toc \|z kostenfrei
912			\|a GBV_USEFLAG_A
912			\|a SYSFLAG_A
912			\|a GBV_DOAJ
912			\|a GBV_ILN_20
912			\|a GBV_ILN_22
912			\|a GBV_ILN_23
912			\|a GBV_ILN_24
912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_95
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_151
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_206
912			\|a GBV_ILN_213
912			\|a GBV_ILN_230
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_602
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4367
912			\|a GBV_ILN_4700
951			\|a AR
952			\|d 10 \|j 2021 \|e 7, p 794

Indexfelder

author_variant	e l el p n pn j m b jmb t r tr r g m rgm e b b ebb
matchkey_str	article:20760817:2021----::oprsnfeooiaadoeuaasyfrbroelipceid
hierarchy_sort_str	2021
publishDate	2021
allfields	10.3390/pathogens10070794 doi (DE-627)DOAJ079363393 (DE-599)DOAJ9caaff90501140c997dca552bb963043 DE-627 ger DE-627 rakwb eng Erin Lashnits verfasserin aut Comparison of Serological and Molecular Assays for <i<Bartonella</i< Species in Dogs with Hemangiosarcoma 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Currently, a gold standard diagnostic test for <i<Bartonella</i< infection in dogs is lacking. This represents a critical limitation for the development and evaluation of new diagnostic tests, as well as for the diagnosis of, and research on, bartonellosis in dogs. This retrospective observational study aims to compare the results of commonly performed and newly-reported <i<Bartonella</i< spp. diagnostic tests in banked clinical specimens from 90 dogs with hemangiosarcoma (HSA) using composite reference standard (CRS) and random effects latent class analysis (RE-LCA) techniques. Samples from each dog were tested using six serological or molecular diagnostic assays, including indirect fluorescent antibody (IFA) and Western blot (WB) for the detection of antibodies in serum, and qPCR and droplet digital PCR (ddPCR) in blood and fresh frozen tissue biopsy samples (mainly splenic HSA tumors and histopathologically normal spleen or skin/adipose tissue). <i<Bartonella</i< infection prevalence was estimated to be 78% based on the CRS (parallel testing with all six assays), and 64% based on the RE-LCA model. The assay with the highest diagnostic accuracy was qPCR performed on fresh frozen tissue biopsy samples (sensitivity: 94% by RE-LCA and 80% by CRS; specificity: 100%). When comparing newly-reported to traditional <i<Bartonella</i< diagnostic assays, ddPCR was more sensitive for the detection of <i<Bartonella</i< DNA than qPCR when testing blood samples (36% vs. 0%, <i<p</i< < 0.0001). Dogs that were positive on serological assays alone with negative molecular assays were highly unlikely (<3%) to be classified as infected by the RE-LCA model. These data indicate that <i<Bartonella</i< spp. DNA can be PCR amplified from fresh frozen tissues from a majority of dogs with HSA using both qPCR and ddPCR, supporting the use of these methods for future controlled studies comparing the prevalence of <i<Bartonella</i< spp. DNA in the tissue of dogs with HSA to that of unaffected controls. diagnostic testing ddPCR PCR serology sensitivity specificity Medicine R Pradeep Neupane verfasserin aut Julie M. Bradley verfasserin aut Toni Richardson verfasserin aut Ricardo G. Maggi verfasserin aut Edward B. Breitschwerdt verfasserin aut In Pathogens MDPI AG, 2012 10(2021), 7, p 794 (DE-627)732627885 (DE-600)2695572-6 20760817 nnns volume:10 year:2021 number:7, p 794 https://doi.org/10.3390/pathogens10070794 kostenfrei https://doaj.org/article/9caaff90501140c997dca552bb963043 kostenfrei https://www.mdpi.com/2076-0817/10/7/794 kostenfrei https://doaj.org/toc/2076-0817 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 7, p 794
spelling	10.3390/pathogens10070794 doi (DE-627)DOAJ079363393 (DE-599)DOAJ9caaff90501140c997dca552bb963043 DE-627 ger DE-627 rakwb eng Erin Lashnits verfasserin aut Comparison of Serological and Molecular Assays for <i<Bartonella</i< Species in Dogs with Hemangiosarcoma 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Currently, a gold standard diagnostic test for <i<Bartonella</i< infection in dogs is lacking. This represents a critical limitation for the development and evaluation of new diagnostic tests, as well as for the diagnosis of, and research on, bartonellosis in dogs. This retrospective observational study aims to compare the results of commonly performed and newly-reported <i<Bartonella</i< spp. diagnostic tests in banked clinical specimens from 90 dogs with hemangiosarcoma (HSA) using composite reference standard (CRS) and random effects latent class analysis (RE-LCA) techniques. Samples from each dog were tested using six serological or molecular diagnostic assays, including indirect fluorescent antibody (IFA) and Western blot (WB) for the detection of antibodies in serum, and qPCR and droplet digital PCR (ddPCR) in blood and fresh frozen tissue biopsy samples (mainly splenic HSA tumors and histopathologically normal spleen or skin/adipose tissue). <i<Bartonella</i< infection prevalence was estimated to be 78% based on the CRS (parallel testing with all six assays), and 64% based on the RE-LCA model. The assay with the highest diagnostic accuracy was qPCR performed on fresh frozen tissue biopsy samples (sensitivity: 94% by RE-LCA and 80% by CRS; specificity: 100%). When comparing newly-reported to traditional <i<Bartonella</i< diagnostic assays, ddPCR was more sensitive for the detection of <i<Bartonella</i< DNA than qPCR when testing blood samples (36% vs. 0%, <i<p</i< < 0.0001). Dogs that were positive on serological assays alone with negative molecular assays were highly unlikely (<3%) to be classified as infected by the RE-LCA model. These data indicate that <i<Bartonella</i< spp. DNA can be PCR amplified from fresh frozen tissues from a majority of dogs with HSA using both qPCR and ddPCR, supporting the use of these methods for future controlled studies comparing the prevalence of <i<Bartonella</i< spp. DNA in the tissue of dogs with HSA to that of unaffected controls. diagnostic testing ddPCR PCR serology sensitivity specificity Medicine R Pradeep Neupane verfasserin aut Julie M. Bradley verfasserin aut Toni Richardson verfasserin aut Ricardo G. Maggi verfasserin aut Edward B. Breitschwerdt verfasserin aut In Pathogens MDPI AG, 2012 10(2021), 7, p 794 (DE-627)732627885 (DE-600)2695572-6 20760817 nnns volume:10 year:2021 number:7, p 794 https://doi.org/10.3390/pathogens10070794 kostenfrei https://doaj.org/article/9caaff90501140c997dca552bb963043 kostenfrei https://www.mdpi.com/2076-0817/10/7/794 kostenfrei https://doaj.org/toc/2076-0817 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 7, p 794
allfields_unstemmed	10.3390/pathogens10070794 doi (DE-627)DOAJ079363393 (DE-599)DOAJ9caaff90501140c997dca552bb963043 DE-627 ger DE-627 rakwb eng Erin Lashnits verfasserin aut Comparison of Serological and Molecular Assays for <i<Bartonella</i< Species in Dogs with Hemangiosarcoma 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Currently, a gold standard diagnostic test for <i<Bartonella</i< infection in dogs is lacking. This represents a critical limitation for the development and evaluation of new diagnostic tests, as well as for the diagnosis of, and research on, bartonellosis in dogs. This retrospective observational study aims to compare the results of commonly performed and newly-reported <i<Bartonella</i< spp. diagnostic tests in banked clinical specimens from 90 dogs with hemangiosarcoma (HSA) using composite reference standard (CRS) and random effects latent class analysis (RE-LCA) techniques. Samples from each dog were tested using six serological or molecular diagnostic assays, including indirect fluorescent antibody (IFA) and Western blot (WB) for the detection of antibodies in serum, and qPCR and droplet digital PCR (ddPCR) in blood and fresh frozen tissue biopsy samples (mainly splenic HSA tumors and histopathologically normal spleen or skin/adipose tissue). <i<Bartonella</i< infection prevalence was estimated to be 78% based on the CRS (parallel testing with all six assays), and 64% based on the RE-LCA model. The assay with the highest diagnostic accuracy was qPCR performed on fresh frozen tissue biopsy samples (sensitivity: 94% by RE-LCA and 80% by CRS; specificity: 100%). When comparing newly-reported to traditional <i<Bartonella</i< diagnostic assays, ddPCR was more sensitive for the detection of <i<Bartonella</i< DNA than qPCR when testing blood samples (36% vs. 0%, <i<p</i< < 0.0001). Dogs that were positive on serological assays alone with negative molecular assays were highly unlikely (<3%) to be classified as infected by the RE-LCA model. These data indicate that <i<Bartonella</i< spp. DNA can be PCR amplified from fresh frozen tissues from a majority of dogs with HSA using both qPCR and ddPCR, supporting the use of these methods for future controlled studies comparing the prevalence of <i<Bartonella</i< spp. DNA in the tissue of dogs with HSA to that of unaffected controls. diagnostic testing ddPCR PCR serology sensitivity specificity Medicine R Pradeep Neupane verfasserin aut Julie M. Bradley verfasserin aut Toni Richardson verfasserin aut Ricardo G. Maggi verfasserin aut Edward B. Breitschwerdt verfasserin aut In Pathogens MDPI AG, 2012 10(2021), 7, p 794 (DE-627)732627885 (DE-600)2695572-6 20760817 nnns volume:10 year:2021 number:7, p 794 https://doi.org/10.3390/pathogens10070794 kostenfrei https://doaj.org/article/9caaff90501140c997dca552bb963043 kostenfrei https://www.mdpi.com/2076-0817/10/7/794 kostenfrei https://doaj.org/toc/2076-0817 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 7, p 794
allfieldsGer	10.3390/pathogens10070794 doi (DE-627)DOAJ079363393 (DE-599)DOAJ9caaff90501140c997dca552bb963043 DE-627 ger DE-627 rakwb eng Erin Lashnits verfasserin aut Comparison of Serological and Molecular Assays for <i<Bartonella</i< Species in Dogs with Hemangiosarcoma 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Currently, a gold standard diagnostic test for <i<Bartonella</i< infection in dogs is lacking. This represents a critical limitation for the development and evaluation of new diagnostic tests, as well as for the diagnosis of, and research on, bartonellosis in dogs. This retrospective observational study aims to compare the results of commonly performed and newly-reported <i<Bartonella</i< spp. diagnostic tests in banked clinical specimens from 90 dogs with hemangiosarcoma (HSA) using composite reference standard (CRS) and random effects latent class analysis (RE-LCA) techniques. Samples from each dog were tested using six serological or molecular diagnostic assays, including indirect fluorescent antibody (IFA) and Western blot (WB) for the detection of antibodies in serum, and qPCR and droplet digital PCR (ddPCR) in blood and fresh frozen tissue biopsy samples (mainly splenic HSA tumors and histopathologically normal spleen or skin/adipose tissue). <i<Bartonella</i< infection prevalence was estimated to be 78% based on the CRS (parallel testing with all six assays), and 64% based on the RE-LCA model. The assay with the highest diagnostic accuracy was qPCR performed on fresh frozen tissue biopsy samples (sensitivity: 94% by RE-LCA and 80% by CRS; specificity: 100%). When comparing newly-reported to traditional <i<Bartonella</i< diagnostic assays, ddPCR was more sensitive for the detection of <i<Bartonella</i< DNA than qPCR when testing blood samples (36% vs. 0%, <i<p</i< < 0.0001). Dogs that were positive on serological assays alone with negative molecular assays were highly unlikely (<3%) to be classified as infected by the RE-LCA model. These data indicate that <i<Bartonella</i< spp. DNA can be PCR amplified from fresh frozen tissues from a majority of dogs with HSA using both qPCR and ddPCR, supporting the use of these methods for future controlled studies comparing the prevalence of <i<Bartonella</i< spp. DNA in the tissue of dogs with HSA to that of unaffected controls. diagnostic testing ddPCR PCR serology sensitivity specificity Medicine R Pradeep Neupane verfasserin aut Julie M. Bradley verfasserin aut Toni Richardson verfasserin aut Ricardo G. Maggi verfasserin aut Edward B. Breitschwerdt verfasserin aut In Pathogens MDPI AG, 2012 10(2021), 7, p 794 (DE-627)732627885 (DE-600)2695572-6 20760817 nnns volume:10 year:2021 number:7, p 794 https://doi.org/10.3390/pathogens10070794 kostenfrei https://doaj.org/article/9caaff90501140c997dca552bb963043 kostenfrei https://www.mdpi.com/2076-0817/10/7/794 kostenfrei https://doaj.org/toc/2076-0817 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 7, p 794
allfieldsSound	10.3390/pathogens10070794 doi (DE-627)DOAJ079363393 (DE-599)DOAJ9caaff90501140c997dca552bb963043 DE-627 ger DE-627 rakwb eng Erin Lashnits verfasserin aut Comparison of Serological and Molecular Assays for <i<Bartonella</i< Species in Dogs with Hemangiosarcoma 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Currently, a gold standard diagnostic test for <i<Bartonella</i< infection in dogs is lacking. This represents a critical limitation for the development and evaluation of new diagnostic tests, as well as for the diagnosis of, and research on, bartonellosis in dogs. This retrospective observational study aims to compare the results of commonly performed and newly-reported <i<Bartonella</i< spp. diagnostic tests in banked clinical specimens from 90 dogs with hemangiosarcoma (HSA) using composite reference standard (CRS) and random effects latent class analysis (RE-LCA) techniques. Samples from each dog were tested using six serological or molecular diagnostic assays, including indirect fluorescent antibody (IFA) and Western blot (WB) for the detection of antibodies in serum, and qPCR and droplet digital PCR (ddPCR) in blood and fresh frozen tissue biopsy samples (mainly splenic HSA tumors and histopathologically normal spleen or skin/adipose tissue). <i<Bartonella</i< infection prevalence was estimated to be 78% based on the CRS (parallel testing with all six assays), and 64% based on the RE-LCA model. The assay with the highest diagnostic accuracy was qPCR performed on fresh frozen tissue biopsy samples (sensitivity: 94% by RE-LCA and 80% by CRS; specificity: 100%). When comparing newly-reported to traditional <i<Bartonella</i< diagnostic assays, ddPCR was more sensitive for the detection of <i<Bartonella</i< DNA than qPCR when testing blood samples (36% vs. 0%, <i<p</i< < 0.0001). Dogs that were positive on serological assays alone with negative molecular assays were highly unlikely (<3%) to be classified as infected by the RE-LCA model. These data indicate that <i<Bartonella</i< spp. DNA can be PCR amplified from fresh frozen tissues from a majority of dogs with HSA using both qPCR and ddPCR, supporting the use of these methods for future controlled studies comparing the prevalence of <i<Bartonella</i< spp. DNA in the tissue of dogs with HSA to that of unaffected controls. diagnostic testing ddPCR PCR serology sensitivity specificity Medicine R Pradeep Neupane verfasserin aut Julie M. Bradley verfasserin aut Toni Richardson verfasserin aut Ricardo G. Maggi verfasserin aut Edward B. Breitschwerdt verfasserin aut In Pathogens MDPI AG, 2012 10(2021), 7, p 794 (DE-627)732627885 (DE-600)2695572-6 20760817 nnns volume:10 year:2021 number:7, p 794 https://doi.org/10.3390/pathogens10070794 kostenfrei https://doaj.org/article/9caaff90501140c997dca552bb963043 kostenfrei https://www.mdpi.com/2076-0817/10/7/794 kostenfrei https://doaj.org/toc/2076-0817 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 7, p 794
language	English
source	In Pathogens 10(2021), 7, p 794 volume:10 year:2021 number:7, p 794
sourceStr	In Pathogens 10(2021), 7, p 794 volume:10 year:2021 number:7, p 794
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	diagnostic testing ddPCR PCR serology sensitivity specificity Medicine R
isfreeaccess_bool	true
container_title	Pathogens
authorswithroles_txt_mv	Erin Lashnits @@aut@@ Pradeep Neupane @@aut@@ Julie M. Bradley @@aut@@ Toni Richardson @@aut@@ Ricardo G. Maggi @@aut@@ Edward B. Breitschwerdt @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	732627885
id	DOAJ079363393
language_de	englisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ079363393</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20240412173508.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230307s2021 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.3390/pathogens10070794</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ079363393</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ9caaff90501140c997dca552bb963043</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Erin Lashnits</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Comparison of Serological and Molecular Assays for <i<Bartonella</i< Species in Dogs with Hemangiosarcoma</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2021</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Currently, a gold standard diagnostic test for <i<Bartonella</i< infection in dogs is lacking. This represents a critical limitation for the development and evaluation of new diagnostic tests, as well as for the diagnosis of, and research on, bartonellosis in dogs. This retrospective observational study aims to compare the results of commonly performed and newly-reported <i<Bartonella</i< spp. diagnostic tests in banked clinical specimens from 90 dogs with hemangiosarcoma (HSA) using composite reference standard (CRS) and random effects latent class analysis (RE-LCA) techniques. Samples from each dog were tested using six serological or molecular diagnostic assays, including indirect fluorescent antibody (IFA) and Western blot (WB) for the detection of antibodies in serum, and qPCR and droplet digital PCR (ddPCR) in blood and fresh frozen tissue biopsy samples (mainly splenic HSA tumors and histopathologically normal spleen or skin/adipose tissue). <i<Bartonella</i< infection prevalence was estimated to be 78% based on the CRS (parallel testing with all six assays), and 64% based on the RE-LCA model. The assay with the highest diagnostic accuracy was qPCR performed on fresh frozen tissue biopsy samples (sensitivity: 94% by RE-LCA and 80% by CRS; specificity: 100%). When comparing newly-reported to traditional <i<Bartonella</i< diagnostic assays, ddPCR was more sensitive for the detection of <i<Bartonella</i< DNA than qPCR when testing blood samples (36% vs. 0%, <i<p</i< < 0.0001). Dogs that were positive on serological assays alone with negative molecular assays were highly unlikely (<3%) to be classified as infected by the RE-LCA model. These data indicate that <i<Bartonella</i< spp. DNA can be PCR amplified from fresh frozen tissues from a majority of dogs with HSA using both qPCR and ddPCR, supporting the use of these methods for future controlled studies comparing the prevalence of <i<Bartonella</i< spp. DNA in the tissue of dogs with HSA to that of unaffected controls.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">diagnostic testing</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">ddPCR</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">PCR</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">serology</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">sensitivity</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">specificity</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Medicine</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">R</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Pradeep Neupane</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Julie M. Bradley</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Toni Richardson</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Ricardo G. Maggi</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Edward B. Breitschwerdt</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Pathogens</subfield><subfield code="d">MDPI AG, 2012</subfield><subfield code="g">10(2021), 7, p 794</subfield><subfield code="w">(DE-627)732627885</subfield><subfield code="w">(DE-600)2695572-6</subfield><subfield code="x">20760817</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:10</subfield><subfield code="g">year:2021</subfield><subfield code="g">number:7, p 794</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.3390/pathogens10070794</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/9caaff90501140c997dca552bb963043</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://www.mdpi.com/2076-0817/10/7/794</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/2076-0817</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_206</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">10</subfield><subfield code="j">2021</subfield><subfield code="e">7, p 794</subfield></datafield></record></collection>
author	Erin Lashnits
spellingShingle	Erin Lashnits misc diagnostic testing misc ddPCR misc PCR misc serology misc sensitivity misc specificity misc Medicine misc R Comparison of Serological and Molecular Assays for <i<Bartonella</i< Species in Dogs with Hemangiosarcoma
authorStr	Erin Lashnits
ppnlink_with_tag_str_mv	@@773@@(DE-627)732627885
format	electronic Article
delete_txt_mv	keep
author_role	aut aut aut aut aut aut
collection	DOAJ
remote_str	true
illustrated	Not Illustrated
issn	20760817
topic_title	Comparison of Serological and Molecular Assays for <i<Bartonella</i< Species in Dogs with Hemangiosarcoma diagnostic testing ddPCR PCR serology sensitivity specificity
topic	misc diagnostic testing misc ddPCR misc PCR misc serology misc sensitivity misc specificity misc Medicine misc R
topic_unstemmed	misc diagnostic testing misc ddPCR misc PCR misc serology misc sensitivity misc specificity misc Medicine misc R
topic_browse	misc diagnostic testing misc ddPCR misc PCR misc serology misc sensitivity misc specificity misc Medicine misc R
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
format_main_str_mv	Text Zeitschrift/Artikel
carriertype_str_mv	cr
hierarchy_parent_title	Pathogens
hierarchy_parent_id	732627885
hierarchy_top_title	Pathogens
isfreeaccess_txt	true
familylinks_str_mv	(DE-627)732627885 (DE-600)2695572-6
title	Comparison of Serological and Molecular Assays for <i<Bartonella</i< Species in Dogs with Hemangiosarcoma
ctrlnum	(DE-627)DOAJ079363393 (DE-599)DOAJ9caaff90501140c997dca552bb963043
title_full	Comparison of Serological and Molecular Assays for <i<Bartonella</i< Species in Dogs with Hemangiosarcoma
author_sort	Erin Lashnits
journal	Pathogens
journalStr	Pathogens
lang_code	eng
isOA_bool	true
recordtype	marc
publishDateSort	2021
contenttype_str_mv	txt
author_browse	Erin Lashnits Pradeep Neupane Julie M. Bradley Toni Richardson Ricardo G. Maggi Edward B. Breitschwerdt
container_volume	10
format_se	Elektronische Aufsätze
author-letter	Erin Lashnits
doi_str_mv	10.3390/pathogens10070794
author2-role	verfasserin
title_sort	comparison of serological and molecular assays for <i<bartonella</i< species in dogs with hemangiosarcoma
title_auth	Comparison of Serological and Molecular Assays for <i<Bartonella</i< Species in Dogs with Hemangiosarcoma
abstract	Currently, a gold standard diagnostic test for <i<Bartonella</i< infection in dogs is lacking. This represents a critical limitation for the development and evaluation of new diagnostic tests, as well as for the diagnosis of, and research on, bartonellosis in dogs. This retrospective observational study aims to compare the results of commonly performed and newly-reported <i<Bartonella</i< spp. diagnostic tests in banked clinical specimens from 90 dogs with hemangiosarcoma (HSA) using composite reference standard (CRS) and random effects latent class analysis (RE-LCA) techniques. Samples from each dog were tested using six serological or molecular diagnostic assays, including indirect fluorescent antibody (IFA) and Western blot (WB) for the detection of antibodies in serum, and qPCR and droplet digital PCR (ddPCR) in blood and fresh frozen tissue biopsy samples (mainly splenic HSA tumors and histopathologically normal spleen or skin/adipose tissue). <i<Bartonella</i< infection prevalence was estimated to be 78% based on the CRS (parallel testing with all six assays), and 64% based on the RE-LCA model. The assay with the highest diagnostic accuracy was qPCR performed on fresh frozen tissue biopsy samples (sensitivity: 94% by RE-LCA and 80% by CRS; specificity: 100%). When comparing newly-reported to traditional <i<Bartonella</i< diagnostic assays, ddPCR was more sensitive for the detection of <i<Bartonella</i< DNA than qPCR when testing blood samples (36% vs. 0%, <i<p</i< < 0.0001). Dogs that were positive on serological assays alone with negative molecular assays were highly unlikely (<3%) to be classified as infected by the RE-LCA model. These data indicate that <i<Bartonella</i< spp. DNA can be PCR amplified from fresh frozen tissues from a majority of dogs with HSA using both qPCR and ddPCR, supporting the use of these methods for future controlled studies comparing the prevalence of <i<Bartonella</i< spp. DNA in the tissue of dogs with HSA to that of unaffected controls.
abstractGer	Currently, a gold standard diagnostic test for <i<Bartonella</i< infection in dogs is lacking. This represents a critical limitation for the development and evaluation of new diagnostic tests, as well as for the diagnosis of, and research on, bartonellosis in dogs. This retrospective observational study aims to compare the results of commonly performed and newly-reported <i<Bartonella</i< spp. diagnostic tests in banked clinical specimens from 90 dogs with hemangiosarcoma (HSA) using composite reference standard (CRS) and random effects latent class analysis (RE-LCA) techniques. Samples from each dog were tested using six serological or molecular diagnostic assays, including indirect fluorescent antibody (IFA) and Western blot (WB) for the detection of antibodies in serum, and qPCR and droplet digital PCR (ddPCR) in blood and fresh frozen tissue biopsy samples (mainly splenic HSA tumors and histopathologically normal spleen or skin/adipose tissue). <i<Bartonella</i< infection prevalence was estimated to be 78% based on the CRS (parallel testing with all six assays), and 64% based on the RE-LCA model. The assay with the highest diagnostic accuracy was qPCR performed on fresh frozen tissue biopsy samples (sensitivity: 94% by RE-LCA and 80% by CRS; specificity: 100%). When comparing newly-reported to traditional <i<Bartonella</i< diagnostic assays, ddPCR was more sensitive for the detection of <i<Bartonella</i< DNA than qPCR when testing blood samples (36% vs. 0%, <i<p</i< < 0.0001). Dogs that were positive on serological assays alone with negative molecular assays were highly unlikely (<3%) to be classified as infected by the RE-LCA model. These data indicate that <i<Bartonella</i< spp. DNA can be PCR amplified from fresh frozen tissues from a majority of dogs with HSA using both qPCR and ddPCR, supporting the use of these methods for future controlled studies comparing the prevalence of <i<Bartonella</i< spp. DNA in the tissue of dogs with HSA to that of unaffected controls.
abstract_unstemmed	Currently, a gold standard diagnostic test for <i<Bartonella</i< infection in dogs is lacking. This represents a critical limitation for the development and evaluation of new diagnostic tests, as well as for the diagnosis of, and research on, bartonellosis in dogs. This retrospective observational study aims to compare the results of commonly performed and newly-reported <i<Bartonella</i< spp. diagnostic tests in banked clinical specimens from 90 dogs with hemangiosarcoma (HSA) using composite reference standard (CRS) and random effects latent class analysis (RE-LCA) techniques. Samples from each dog were tested using six serological or molecular diagnostic assays, including indirect fluorescent antibody (IFA) and Western blot (WB) for the detection of antibodies in serum, and qPCR and droplet digital PCR (ddPCR) in blood and fresh frozen tissue biopsy samples (mainly splenic HSA tumors and histopathologically normal spleen or skin/adipose tissue). <i<Bartonella</i< infection prevalence was estimated to be 78% based on the CRS (parallel testing with all six assays), and 64% based on the RE-LCA model. The assay with the highest diagnostic accuracy was qPCR performed on fresh frozen tissue biopsy samples (sensitivity: 94% by RE-LCA and 80% by CRS; specificity: 100%). When comparing newly-reported to traditional <i<Bartonella</i< diagnostic assays, ddPCR was more sensitive for the detection of <i<Bartonella</i< DNA than qPCR when testing blood samples (36% vs. 0%, <i<p</i< < 0.0001). Dogs that were positive on serological assays alone with negative molecular assays were highly unlikely (<3%) to be classified as infected by the RE-LCA model. These data indicate that <i<Bartonella</i< spp. DNA can be PCR amplified from fresh frozen tissues from a majority of dogs with HSA using both qPCR and ddPCR, supporting the use of these methods for future controlled studies comparing the prevalence of <i<Bartonella</i< spp. DNA in the tissue of dogs with HSA to that of unaffected controls.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
container_issue	7, p 794
title_short	Comparison of Serological and Molecular Assays for <i<Bartonella</i< Species in Dogs with Hemangiosarcoma
url	https://doi.org/10.3390/pathogens10070794 https://doaj.org/article/9caaff90501140c997dca552bb963043 https://www.mdpi.com/2076-0817/10/7/794 https://doaj.org/toc/2076-0817
remote_bool	true
author2	Pradeep Neupane Julie M. Bradley Toni Richardson Ricardo G. Maggi Edward B. Breitschwerdt
author2Str	Pradeep Neupane Julie M. Bradley Toni Richardson Ricardo G. Maggi Edward B. Breitschwerdt
ppnlink	732627885
mediatype_str_mv	c
isOA_txt	true
hochschulschrift_bool	false
doi_str	10.3390/pathogens10070794
up_date	2024-07-03T23:05:52.808Z
_version_	1803601002165698560
fullrecord_marcxml	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ079363393</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20240412173508.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230307s2021 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.3390/pathogens10070794</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ079363393</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ9caaff90501140c997dca552bb963043</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Erin Lashnits</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Comparison of Serological and Molecular Assays for <i<Bartonella</i< Species in Dogs with Hemangiosarcoma</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2021</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Currently, a gold standard diagnostic test for <i<Bartonella</i< infection in dogs is lacking. This represents a critical limitation for the development and evaluation of new diagnostic tests, as well as for the diagnosis of, and research on, bartonellosis in dogs. This retrospective observational study aims to compare the results of commonly performed and newly-reported <i<Bartonella</i< spp. diagnostic tests in banked clinical specimens from 90 dogs with hemangiosarcoma (HSA) using composite reference standard (CRS) and random effects latent class analysis (RE-LCA) techniques. Samples from each dog were tested using six serological or molecular diagnostic assays, including indirect fluorescent antibody (IFA) and Western blot (WB) for the detection of antibodies in serum, and qPCR and droplet digital PCR (ddPCR) in blood and fresh frozen tissue biopsy samples (mainly splenic HSA tumors and histopathologically normal spleen or skin/adipose tissue). <i<Bartonella</i< infection prevalence was estimated to be 78% based on the CRS (parallel testing with all six assays), and 64% based on the RE-LCA model. The assay with the highest diagnostic accuracy was qPCR performed on fresh frozen tissue biopsy samples (sensitivity: 94% by RE-LCA and 80% by CRS; specificity: 100%). When comparing newly-reported to traditional <i<Bartonella</i< diagnostic assays, ddPCR was more sensitive for the detection of <i<Bartonella</i< DNA than qPCR when testing blood samples (36% vs. 0%, <i<p</i< < 0.0001). Dogs that were positive on serological assays alone with negative molecular assays were highly unlikely (<3%) to be classified as infected by the RE-LCA model. These data indicate that <i<Bartonella</i< spp. DNA can be PCR amplified from fresh frozen tissues from a majority of dogs with HSA using both qPCR and ddPCR, supporting the use of these methods for future controlled studies comparing the prevalence of <i<Bartonella</i< spp. DNA in the tissue of dogs with HSA to that of unaffected controls.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">diagnostic testing</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">ddPCR</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">PCR</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">serology</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">sensitivity</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">specificity</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Medicine</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">R</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Pradeep Neupane</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Julie M. Bradley</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Toni Richardson</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Ricardo G. Maggi</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Edward B. Breitschwerdt</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Pathogens</subfield><subfield code="d">MDPI AG, 2012</subfield><subfield code="g">10(2021), 7, p 794</subfield><subfield code="w">(DE-627)732627885</subfield><subfield code="w">(DE-600)2695572-6</subfield><subfield code="x">20760817</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:10</subfield><subfield code="g">year:2021</subfield><subfield code="g">number:7, p 794</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.3390/pathogens10070794</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/9caaff90501140c997dca552bb963043</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://www.mdpi.com/2076-0817/10/7/794</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/2076-0817</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_206</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">10</subfield><subfield code="j">2021</subfield><subfield code="e">7, p 794</subfield></datafield></record></collection>
score	7.3982544

Nicht das Richtige dabei?

Schreiben Sie uns!

Comparison of Serological and Molecular Assays for <i<Bartonella</i< Species in Dogs with Hemangiosarcoma

Nicht das Richtige dabei?

Zugang & Verfügbarkeit

Vorhandene Bände

Nicht das Richtige dabei?