Peripheral IFN-ɣ Production after Blood Stimulation with Different Mycobacterial Antigens in Goats Vaccinated against Paratuberculosis

Vaccination can be an efficient method for the control of paratuberculosis in ruminants. However, the official tuberculosis control tests cross-interfere with the animals vaccinated against paratuberculosis. In order to test and compare new antigens that could solve this problem, the production of i...
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Autor*in:	Miguel Fernández [verfasserIn] Marcos Royo [verfasserIn] Noive Arteche-Villasol [verfasserIn] M. Carmen Ferreras [verfasserIn] Julio Benavides [verfasserIn] Valentín Pérez [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	paratuberculosis vaccination ruminants interferon-gamma immune response

Übergeordnetes Werk:	In: Vaccines - MDPI AG, 2013, 10(2022), 10, p 1709
Übergeordnetes Werk:	volume:10 ; year:2022 ; number:10, p 1709

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/vaccines10101709

Katalog-ID:	DOAJ079436234

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520			\|a Vaccination can be an efficient method for the control of paratuberculosis in ruminants. However, the official tuberculosis control tests cross-interfere with the animals vaccinated against paratuberculosis. In order to test and compare new antigens that could solve this problem, the production of interferon-gamma (IFN-γ) in peripheral blood at different post-vaccination days in experimental kids and adult goats, in field conditions, using the avian and bovine purified protein derivative (PPD), the johnin, two peptide cocktails of <i<Mycobacterium bovis</i< (PC-EC and PC-HP) and the antigens VK 055 and VK 067 of <i<Mycobacterium avium</i< subspecies <i<paratuberculosis</i< (Map) has been analyzed in vitro. The non-specific production of IFN-γ was observed after blood stimulation with the PC-EC and PC-HP cocktail in any sample from vaccinated animals, whereas it was detected when bovine PPD was used. These results support the possible use of these new <i<Mycobacterium bovis</i< antigens in the in the differentiation of animals vaccinated against paratuberculosis or infected with tuberculosis by improving the specificity of bovine PPD. In contrast, the two Map antigens tested in this study did not improve the sensitivity of johnin or avian PPD in the detection of vaccinated or Map-infected goats.
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allfields	10.3390/vaccines10101709 doi (DE-627)DOAJ079436234 (DE-599)DOAJ4bc69c0893524f489f4576bc0600b70a DE-627 ger DE-627 rakwb eng Miguel Fernández verfasserin aut Peripheral IFN-ɣ Production after Blood Stimulation with Different Mycobacterial Antigens in Goats Vaccinated against Paratuberculosis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Vaccination can be an efficient method for the control of paratuberculosis in ruminants. However, the official tuberculosis control tests cross-interfere with the animals vaccinated against paratuberculosis. In order to test and compare new antigens that could solve this problem, the production of interferon-gamma (IFN-γ) in peripheral blood at different post-vaccination days in experimental kids and adult goats, in field conditions, using the avian and bovine purified protein derivative (PPD), the johnin, two peptide cocktails of <i<Mycobacterium bovis</i< (PC-EC and PC-HP) and the antigens VK 055 and VK 067 of <i<Mycobacterium avium</i< subspecies <i<paratuberculosis</i< (Map) has been analyzed in vitro. The non-specific production of IFN-γ was observed after blood stimulation with the PC-EC and PC-HP cocktail in any sample from vaccinated animals, whereas it was detected when bovine PPD was used. These results support the possible use of these new <i<Mycobacterium bovis</i< antigens in the in the differentiation of animals vaccinated against paratuberculosis or infected with tuberculosis by improving the specificity of bovine PPD. In contrast, the two Map antigens tested in this study did not improve the sensitivity of johnin or avian PPD in the detection of vaccinated or Map-infected goats. paratuberculosis vaccination ruminants <i<Mycobacterium avium</i< subspecies <i<paratuberculosis</i< interferon-gamma immune response Medicine R Marcos Royo verfasserin aut Noive Arteche-Villasol verfasserin aut M. Carmen Ferreras verfasserin aut Julio Benavides verfasserin aut Valentín Pérez verfasserin aut In Vaccines MDPI AG, 2013 10(2022), 10, p 1709 (DE-627)736559205 (DE-600)2703319-3 2076393X nnns volume:10 year:2022 number:10, p 1709 https://doi.org/10.3390/vaccines10101709 kostenfrei https://doaj.org/article/4bc69c0893524f489f4576bc0600b70a kostenfrei https://www.mdpi.com/2076-393X/10/10/1709 kostenfrei https://doaj.org/toc/2076-393X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2022 10, p 1709
spelling	10.3390/vaccines10101709 doi (DE-627)DOAJ079436234 (DE-599)DOAJ4bc69c0893524f489f4576bc0600b70a DE-627 ger DE-627 rakwb eng Miguel Fernández verfasserin aut Peripheral IFN-ɣ Production after Blood Stimulation with Different Mycobacterial Antigens in Goats Vaccinated against Paratuberculosis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Vaccination can be an efficient method for the control of paratuberculosis in ruminants. However, the official tuberculosis control tests cross-interfere with the animals vaccinated against paratuberculosis. In order to test and compare new antigens that could solve this problem, the production of interferon-gamma (IFN-γ) in peripheral blood at different post-vaccination days in experimental kids and adult goats, in field conditions, using the avian and bovine purified protein derivative (PPD), the johnin, two peptide cocktails of <i<Mycobacterium bovis</i< (PC-EC and PC-HP) and the antigens VK 055 and VK 067 of <i<Mycobacterium avium</i< subspecies <i<paratuberculosis</i< (Map) has been analyzed in vitro. The non-specific production of IFN-γ was observed after blood stimulation with the PC-EC and PC-HP cocktail in any sample from vaccinated animals, whereas it was detected when bovine PPD was used. These results support the possible use of these new <i<Mycobacterium bovis</i< antigens in the in the differentiation of animals vaccinated against paratuberculosis or infected with tuberculosis by improving the specificity of bovine PPD. In contrast, the two Map antigens tested in this study did not improve the sensitivity of johnin or avian PPD in the detection of vaccinated or Map-infected goats. paratuberculosis vaccination ruminants <i<Mycobacterium avium</i< subspecies <i<paratuberculosis</i< interferon-gamma immune response Medicine R Marcos Royo verfasserin aut Noive Arteche-Villasol verfasserin aut M. Carmen Ferreras verfasserin aut Julio Benavides verfasserin aut Valentín Pérez verfasserin aut In Vaccines MDPI AG, 2013 10(2022), 10, p 1709 (DE-627)736559205 (DE-600)2703319-3 2076393X nnns volume:10 year:2022 number:10, p 1709 https://doi.org/10.3390/vaccines10101709 kostenfrei https://doaj.org/article/4bc69c0893524f489f4576bc0600b70a kostenfrei https://www.mdpi.com/2076-393X/10/10/1709 kostenfrei https://doaj.org/toc/2076-393X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2022 10, p 1709
allfields_unstemmed	10.3390/vaccines10101709 doi (DE-627)DOAJ079436234 (DE-599)DOAJ4bc69c0893524f489f4576bc0600b70a DE-627 ger DE-627 rakwb eng Miguel Fernández verfasserin aut Peripheral IFN-ɣ Production after Blood Stimulation with Different Mycobacterial Antigens in Goats Vaccinated against Paratuberculosis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Vaccination can be an efficient method for the control of paratuberculosis in ruminants. However, the official tuberculosis control tests cross-interfere with the animals vaccinated against paratuberculosis. In order to test and compare new antigens that could solve this problem, the production of interferon-gamma (IFN-γ) in peripheral blood at different post-vaccination days in experimental kids and adult goats, in field conditions, using the avian and bovine purified protein derivative (PPD), the johnin, two peptide cocktails of <i<Mycobacterium bovis</i< (PC-EC and PC-HP) and the antigens VK 055 and VK 067 of <i<Mycobacterium avium</i< subspecies <i<paratuberculosis</i< (Map) has been analyzed in vitro. The non-specific production of IFN-γ was observed after blood stimulation with the PC-EC and PC-HP cocktail in any sample from vaccinated animals, whereas it was detected when bovine PPD was used. These results support the possible use of these new <i<Mycobacterium bovis</i< antigens in the in the differentiation of animals vaccinated against paratuberculosis or infected with tuberculosis by improving the specificity of bovine PPD. In contrast, the two Map antigens tested in this study did not improve the sensitivity of johnin or avian PPD in the detection of vaccinated or Map-infected goats. paratuberculosis vaccination ruminants <i<Mycobacterium avium</i< subspecies <i<paratuberculosis</i< interferon-gamma immune response Medicine R Marcos Royo verfasserin aut Noive Arteche-Villasol verfasserin aut M. Carmen Ferreras verfasserin aut Julio Benavides verfasserin aut Valentín Pérez verfasserin aut In Vaccines MDPI AG, 2013 10(2022), 10, p 1709 (DE-627)736559205 (DE-600)2703319-3 2076393X nnns volume:10 year:2022 number:10, p 1709 https://doi.org/10.3390/vaccines10101709 kostenfrei https://doaj.org/article/4bc69c0893524f489f4576bc0600b70a kostenfrei https://www.mdpi.com/2076-393X/10/10/1709 kostenfrei https://doaj.org/toc/2076-393X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2022 10, p 1709
allfieldsGer	10.3390/vaccines10101709 doi (DE-627)DOAJ079436234 (DE-599)DOAJ4bc69c0893524f489f4576bc0600b70a DE-627 ger DE-627 rakwb eng Miguel Fernández verfasserin aut Peripheral IFN-ɣ Production after Blood Stimulation with Different Mycobacterial Antigens in Goats Vaccinated against Paratuberculosis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Vaccination can be an efficient method for the control of paratuberculosis in ruminants. However, the official tuberculosis control tests cross-interfere with the animals vaccinated against paratuberculosis. In order to test and compare new antigens that could solve this problem, the production of interferon-gamma (IFN-γ) in peripheral blood at different post-vaccination days in experimental kids and adult goats, in field conditions, using the avian and bovine purified protein derivative (PPD), the johnin, two peptide cocktails of <i<Mycobacterium bovis</i< (PC-EC and PC-HP) and the antigens VK 055 and VK 067 of <i<Mycobacterium avium</i< subspecies <i<paratuberculosis</i< (Map) has been analyzed in vitro. The non-specific production of IFN-γ was observed after blood stimulation with the PC-EC and PC-HP cocktail in any sample from vaccinated animals, whereas it was detected when bovine PPD was used. These results support the possible use of these new <i<Mycobacterium bovis</i< antigens in the in the differentiation of animals vaccinated against paratuberculosis or infected with tuberculosis by improving the specificity of bovine PPD. In contrast, the two Map antigens tested in this study did not improve the sensitivity of johnin or avian PPD in the detection of vaccinated or Map-infected goats. paratuberculosis vaccination ruminants <i<Mycobacterium avium</i< subspecies <i<paratuberculosis</i< interferon-gamma immune response Medicine R Marcos Royo verfasserin aut Noive Arteche-Villasol verfasserin aut M. Carmen Ferreras verfasserin aut Julio Benavides verfasserin aut Valentín Pérez verfasserin aut In Vaccines MDPI AG, 2013 10(2022), 10, p 1709 (DE-627)736559205 (DE-600)2703319-3 2076393X nnns volume:10 year:2022 number:10, p 1709 https://doi.org/10.3390/vaccines10101709 kostenfrei https://doaj.org/article/4bc69c0893524f489f4576bc0600b70a kostenfrei https://www.mdpi.com/2076-393X/10/10/1709 kostenfrei https://doaj.org/toc/2076-393X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2022 10, p 1709
allfieldsSound	10.3390/vaccines10101709 doi (DE-627)DOAJ079436234 (DE-599)DOAJ4bc69c0893524f489f4576bc0600b70a DE-627 ger DE-627 rakwb eng Miguel Fernández verfasserin aut Peripheral IFN-ɣ Production after Blood Stimulation with Different Mycobacterial Antigens in Goats Vaccinated against Paratuberculosis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Vaccination can be an efficient method for the control of paratuberculosis in ruminants. However, the official tuberculosis control tests cross-interfere with the animals vaccinated against paratuberculosis. In order to test and compare new antigens that could solve this problem, the production of interferon-gamma (IFN-γ) in peripheral blood at different post-vaccination days in experimental kids and adult goats, in field conditions, using the avian and bovine purified protein derivative (PPD), the johnin, two peptide cocktails of <i<Mycobacterium bovis</i< (PC-EC and PC-HP) and the antigens VK 055 and VK 067 of <i<Mycobacterium avium</i< subspecies <i<paratuberculosis</i< (Map) has been analyzed in vitro. The non-specific production of IFN-γ was observed after blood stimulation with the PC-EC and PC-HP cocktail in any sample from vaccinated animals, whereas it was detected when bovine PPD was used. These results support the possible use of these new <i<Mycobacterium bovis</i< antigens in the in the differentiation of animals vaccinated against paratuberculosis or infected with tuberculosis by improving the specificity of bovine PPD. In contrast, the two Map antigens tested in this study did not improve the sensitivity of johnin or avian PPD in the detection of vaccinated or Map-infected goats. paratuberculosis vaccination ruminants <i<Mycobacterium avium</i< subspecies <i<paratuberculosis</i< interferon-gamma immune response Medicine R Marcos Royo verfasserin aut Noive Arteche-Villasol verfasserin aut M. Carmen Ferreras verfasserin aut Julio Benavides verfasserin aut Valentín Pérez verfasserin aut In Vaccines MDPI AG, 2013 10(2022), 10, p 1709 (DE-627)736559205 (DE-600)2703319-3 2076393X nnns volume:10 year:2022 number:10, p 1709 https://doi.org/10.3390/vaccines10101709 kostenfrei https://doaj.org/article/4bc69c0893524f489f4576bc0600b70a kostenfrei https://www.mdpi.com/2076-393X/10/10/1709 kostenfrei https://doaj.org/toc/2076-393X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2022 10, p 1709
language	English
source	In Vaccines 10(2022), 10, p 1709 volume:10 year:2022 number:10, p 1709
sourceStr	In Vaccines 10(2022), 10, p 1709 volume:10 year:2022 number:10, p 1709
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	paratuberculosis vaccination ruminants <i<Mycobacterium avium</i< subspecies <i<paratuberculosis</i< interferon-gamma immune response Medicine R
isfreeaccess_bool	true
container_title	Vaccines
authorswithroles_txt_mv	Miguel Fernández @@aut@@ Marcos Royo @@aut@@ Noive Arteche-Villasol @@aut@@ M. Carmen Ferreras @@aut@@ Julio Benavides @@aut@@ Valentín Pérez @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
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author	Miguel Fernández
spellingShingle	Miguel Fernández misc paratuberculosis misc vaccination misc ruminants misc <i<Mycobacterium avium</i< subspecies <i<paratuberculosis</i< misc interferon-gamma misc immune response misc Medicine misc R Peripheral IFN-ɣ Production after Blood Stimulation with Different Mycobacterial Antigens in Goats Vaccinated against Paratuberculosis
authorStr	Miguel Fernández
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topic_title	Peripheral IFN-ɣ Production after Blood Stimulation with Different Mycobacterial Antigens in Goats Vaccinated against Paratuberculosis paratuberculosis vaccination ruminants <i<Mycobacterium avium</i< subspecies <i<paratuberculosis</i< interferon-gamma immune response
topic	misc paratuberculosis misc vaccination misc ruminants misc <i<Mycobacterium avium</i< subspecies <i<paratuberculosis</i< misc interferon-gamma misc immune response misc Medicine misc R
topic_unstemmed	misc paratuberculosis misc vaccination misc ruminants misc <i<Mycobacterium avium</i< subspecies <i<paratuberculosis</i< misc interferon-gamma misc immune response misc Medicine misc R
topic_browse	misc paratuberculosis misc vaccination misc ruminants misc <i<Mycobacterium avium</i< subspecies <i<paratuberculosis</i< misc interferon-gamma misc immune response misc Medicine misc R
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title	Peripheral IFN-ɣ Production after Blood Stimulation with Different Mycobacterial Antigens in Goats Vaccinated against Paratuberculosis
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title_full	Peripheral IFN-ɣ Production after Blood Stimulation with Different Mycobacterial Antigens in Goats Vaccinated against Paratuberculosis
author_sort	Miguel Fernández
journal	Vaccines
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author_browse	Miguel Fernández Marcos Royo Noive Arteche-Villasol M. Carmen Ferreras Julio Benavides Valentín Pérez
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title_sort	peripheral ifn-ɣ production after blood stimulation with different mycobacterial antigens in goats vaccinated against paratuberculosis
title_auth	Peripheral IFN-ɣ Production after Blood Stimulation with Different Mycobacterial Antigens in Goats Vaccinated against Paratuberculosis
abstract	Vaccination can be an efficient method for the control of paratuberculosis in ruminants. However, the official tuberculosis control tests cross-interfere with the animals vaccinated against paratuberculosis. In order to test and compare new antigens that could solve this problem, the production of interferon-gamma (IFN-γ) in peripheral blood at different post-vaccination days in experimental kids and adult goats, in field conditions, using the avian and bovine purified protein derivative (PPD), the johnin, two peptide cocktails of <i<Mycobacterium bovis</i< (PC-EC and PC-HP) and the antigens VK 055 and VK 067 of <i<Mycobacterium avium</i< subspecies <i<paratuberculosis</i< (Map) has been analyzed in vitro. The non-specific production of IFN-γ was observed after blood stimulation with the PC-EC and PC-HP cocktail in any sample from vaccinated animals, whereas it was detected when bovine PPD was used. These results support the possible use of these new <i<Mycobacterium bovis</i< antigens in the in the differentiation of animals vaccinated against paratuberculosis or infected with tuberculosis by improving the specificity of bovine PPD. In contrast, the two Map antigens tested in this study did not improve the sensitivity of johnin or avian PPD in the detection of vaccinated or Map-infected goats.
abstractGer	Vaccination can be an efficient method for the control of paratuberculosis in ruminants. However, the official tuberculosis control tests cross-interfere with the animals vaccinated against paratuberculosis. In order to test and compare new antigens that could solve this problem, the production of interferon-gamma (IFN-γ) in peripheral blood at different post-vaccination days in experimental kids and adult goats, in field conditions, using the avian and bovine purified protein derivative (PPD), the johnin, two peptide cocktails of <i<Mycobacterium bovis</i< (PC-EC and PC-HP) and the antigens VK 055 and VK 067 of <i<Mycobacterium avium</i< subspecies <i<paratuberculosis</i< (Map) has been analyzed in vitro. The non-specific production of IFN-γ was observed after blood stimulation with the PC-EC and PC-HP cocktail in any sample from vaccinated animals, whereas it was detected when bovine PPD was used. These results support the possible use of these new <i<Mycobacterium bovis</i< antigens in the in the differentiation of animals vaccinated against paratuberculosis or infected with tuberculosis by improving the specificity of bovine PPD. In contrast, the two Map antigens tested in this study did not improve the sensitivity of johnin or avian PPD in the detection of vaccinated or Map-infected goats.
abstract_unstemmed	Vaccination can be an efficient method for the control of paratuberculosis in ruminants. However, the official tuberculosis control tests cross-interfere with the animals vaccinated against paratuberculosis. In order to test and compare new antigens that could solve this problem, the production of interferon-gamma (IFN-γ) in peripheral blood at different post-vaccination days in experimental kids and adult goats, in field conditions, using the avian and bovine purified protein derivative (PPD), the johnin, two peptide cocktails of <i<Mycobacterium bovis</i< (PC-EC and PC-HP) and the antigens VK 055 and VK 067 of <i<Mycobacterium avium</i< subspecies <i<paratuberculosis</i< (Map) has been analyzed in vitro. The non-specific production of IFN-γ was observed after blood stimulation with the PC-EC and PC-HP cocktail in any sample from vaccinated animals, whereas it was detected when bovine PPD was used. These results support the possible use of these new <i<Mycobacterium bovis</i< antigens in the in the differentiation of animals vaccinated against paratuberculosis or infected with tuberculosis by improving the specificity of bovine PPD. In contrast, the two Map antigens tested in this study did not improve the sensitivity of johnin or avian PPD in the detection of vaccinated or Map-infected goats.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
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title_short	Peripheral IFN-ɣ Production after Blood Stimulation with Different Mycobacterial Antigens in Goats Vaccinated against Paratuberculosis
url	https://doi.org/10.3390/vaccines10101709 https://doaj.org/article/4bc69c0893524f489f4576bc0600b70a https://www.mdpi.com/2076-393X/10/10/1709 https://doaj.org/toc/2076-393X
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author2	Marcos Royo Noive Arteche-Villasol M. Carmen Ferreras Julio Benavides Valentín Pérez
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up_date	2024-07-03T23:26:56.315Z
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Peripheral IFN-ɣ Production after Blood Stimulation with Different Mycobacterial Antigens in Goats Vaccinated against Paratuberculosis

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