The Analysis of Genetic Polymorphism. The Relationship between Interleukin – 4 Polymorphisms and Intraepithelial Cervical Neoplasia
Objectives: Interleukin 4 plays a critical role in T helper 2 responses to HPV infection and angiogenesis. The present study aim to study the association between the IL4 promoter polymorphism – 590 C<T, respectively VNTR intron 2 polymorphism and cervical intraepithelial neoplasia. Material and m...
Ausführliche Beschreibung
Autor*in: |
Florin STAMATIAN [verfasserIn] Gabriela CARACOSTEA [verfasserIn] Cristina BUTUZA [verfasserIn] Radu POPP [verfasserIn] Daniel MUREŞAN [verfasserIn] Sorana D. BOLBOACĂ [verfasserIn] Ioana ROTAR [verfasserIn] Felicia PETRIŞOR [verfasserIn] Marius FĂRCAŞ [verfasserIn] |
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Format: |
E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2010 |
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Schlagwörter: |
Restriction fragment length polymorphism (RFLP) Polymerase chain reaction (PCR) Cervical intraepithelial neoplasia (CIN) Human papiloma virus (HPV) infection |
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Übergeordnetes Werk: |
In: Applied Medical Informatics - Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, 2009, 27(2010), 3, Seite 43-52 |
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Übergeordnetes Werk: |
volume:27 ; year:2010 ; number:3 ; pages:43-52 |
Links: |
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Katalog-ID: |
DOAJ079490379 |
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245 | 1 | 4 | |a The Analysis of Genetic Polymorphism. The Relationship between Interleukin – 4 Polymorphisms and Intraepithelial Cervical Neoplasia |
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520 | |a Objectives: Interleukin 4 plays a critical role in T helper 2 responses to HPV infection and angiogenesis. The present study aim to study the association between the IL4 promoter polymorphism – 590 C<T, respectively VNTR intron 2 polymorphism and cervical intraepithelial neoplasia. Material and method: We have realized a prospective case controls study that included 128 cases of intraepithelial neoplasia positive for HPV HR testing and 111 controls negative for intraepithelial lesion and also negative for HPV HR. Clinical examination was performed on each patient; blood and cervical sample were obtained. Cervical probes were analyzed regarding cytology and HPV HR testing. From peripheral blood DNA sample was obtain followed by genotype analysis for IL4 -590 C<T using PCR RFLP, respectively IL4 70 bp VNTR determined by PCR. Results: The absolute frequency of genotypes for IL4 -590 C<T was T/T-5, C/T-42, C/C-81 in the cases group respectively T/T-2, C/T-32, C/C-77 in the control group. The chi-square test had a value of 0.983 (p=0.321) while considering the presence of a minimum one single variant allele as a risk factor for cervical cancer, respectively 0.926 (p=0.336) for homozygous variant genotype. Odds ratio was 0.761 (95%CI [0.443-1.306]) while considering C/T+T/T respectively 2R/3R, 2R/2R as a risk factor, and 0.451 (95%CI 95% [0.086-2.374]) - TT respectively 2R/2R as a risk factor. Conclusion: No linear statistical significant association has been found between IL4 polymorphism and cervical neoplasia (p = 0.322). | ||
650 | 4 | |a Genetic polymorphism | |
650 | 4 | |a Restriction fragment length polymorphism (RFLP) | |
650 | 4 | |a Polymerase chain reaction (PCR) | |
650 | 4 | |a Interleukin-4 (IL4) | |
650 | 4 | |a Cervical intraepithelial neoplasia (CIN) | |
650 | 4 | |a Human papiloma virus (HPV) infection | |
650 | 4 | |a Human papiloma virus high risk (HPV-HR) | |
650 | 4 | |a Negative for intraepithelial lesion (NILM) | |
653 | 0 | |a Computer applications to medicine. Medical informatics | |
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700 | 0 | |a Ioana ROTAR |e verfasserin |4 aut | |
700 | 0 | |a Felicia PETRIŞOR |e verfasserin |4 aut | |
700 | 0 | |a Marius FĂRCAŞ |e verfasserin |4 aut | |
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(DE-627)DOAJ079490379 (DE-599)DOAJ1bfb35e137d3452298e3a8af40399f76 DE-627 ger DE-627 rakwb eng R858-859.7 Florin STAMATIAN verfasserin aut The Analysis of Genetic Polymorphism. The Relationship between Interleukin – 4 Polymorphisms and Intraepithelial Cervical Neoplasia 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives: Interleukin 4 plays a critical role in T helper 2 responses to HPV infection and angiogenesis. The present study aim to study the association between the IL4 promoter polymorphism – 590 C<T, respectively VNTR intron 2 polymorphism and cervical intraepithelial neoplasia. Material and method: We have realized a prospective case controls study that included 128 cases of intraepithelial neoplasia positive for HPV HR testing and 111 controls negative for intraepithelial lesion and also negative for HPV HR. Clinical examination was performed on each patient; blood and cervical sample were obtained. Cervical probes were analyzed regarding cytology and HPV HR testing. From peripheral blood DNA sample was obtain followed by genotype analysis for IL4 -590 C<T using PCR RFLP, respectively IL4 70 bp VNTR determined by PCR. Results: The absolute frequency of genotypes for IL4 -590 C<T was T/T-5, C/T-42, C/C-81 in the cases group respectively T/T-2, C/T-32, C/C-77 in the control group. The chi-square test had a value of 0.983 (p=0.321) while considering the presence of a minimum one single variant allele as a risk factor for cervical cancer, respectively 0.926 (p=0.336) for homozygous variant genotype. Odds ratio was 0.761 (95%CI [0.443-1.306]) while considering C/T+T/T respectively 2R/3R, 2R/2R as a risk factor, and 0.451 (95%CI 95% [0.086-2.374]) - TT respectively 2R/2R as a risk factor. Conclusion: No linear statistical significant association has been found between IL4 polymorphism and cervical neoplasia (p = 0.322). Genetic polymorphism Restriction fragment length polymorphism (RFLP) Polymerase chain reaction (PCR) Interleukin-4 (IL4) Cervical intraepithelial neoplasia (CIN) Human papiloma virus (HPV) infection Human papiloma virus high risk (HPV-HR) Negative for intraepithelial lesion (NILM) Computer applications to medicine. Medical informatics Gabriela CARACOSTEA verfasserin aut Cristina BUTUZA verfasserin aut Radu POPP verfasserin aut Daniel MUREŞAN verfasserin aut Sorana D. BOLBOACĂ verfasserin aut Ioana ROTAR verfasserin aut Felicia PETRIŞOR verfasserin aut Marius FĂRCAŞ verfasserin aut In Applied Medical Informatics Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, 2009 27(2010), 3, Seite 43-52 (DE-627)629833281 (DE-600)2558685-3 20677855 nnns volume:27 year:2010 number:3 pages:43-52 https://doaj.org/article/1bfb35e137d3452298e3a8af40399f76 kostenfrei http://ami.info.umfcluj.ro/Full-text/AMI_27_2010/AMI(27)_043_052.pdf kostenfrei https://doaj.org/toc/1224-5593 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 27 2010 3 43-52 |
spelling |
(DE-627)DOAJ079490379 (DE-599)DOAJ1bfb35e137d3452298e3a8af40399f76 DE-627 ger DE-627 rakwb eng R858-859.7 Florin STAMATIAN verfasserin aut The Analysis of Genetic Polymorphism. The Relationship between Interleukin – 4 Polymorphisms and Intraepithelial Cervical Neoplasia 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives: Interleukin 4 plays a critical role in T helper 2 responses to HPV infection and angiogenesis. The present study aim to study the association between the IL4 promoter polymorphism – 590 C<T, respectively VNTR intron 2 polymorphism and cervical intraepithelial neoplasia. Material and method: We have realized a prospective case controls study that included 128 cases of intraepithelial neoplasia positive for HPV HR testing and 111 controls negative for intraepithelial lesion and also negative for HPV HR. Clinical examination was performed on each patient; blood and cervical sample were obtained. Cervical probes were analyzed regarding cytology and HPV HR testing. From peripheral blood DNA sample was obtain followed by genotype analysis for IL4 -590 C<T using PCR RFLP, respectively IL4 70 bp VNTR determined by PCR. Results: The absolute frequency of genotypes for IL4 -590 C<T was T/T-5, C/T-42, C/C-81 in the cases group respectively T/T-2, C/T-32, C/C-77 in the control group. The chi-square test had a value of 0.983 (p=0.321) while considering the presence of a minimum one single variant allele as a risk factor for cervical cancer, respectively 0.926 (p=0.336) for homozygous variant genotype. Odds ratio was 0.761 (95%CI [0.443-1.306]) while considering C/T+T/T respectively 2R/3R, 2R/2R as a risk factor, and 0.451 (95%CI 95% [0.086-2.374]) - TT respectively 2R/2R as a risk factor. Conclusion: No linear statistical significant association has been found between IL4 polymorphism and cervical neoplasia (p = 0.322). Genetic polymorphism Restriction fragment length polymorphism (RFLP) Polymerase chain reaction (PCR) Interleukin-4 (IL4) Cervical intraepithelial neoplasia (CIN) Human papiloma virus (HPV) infection Human papiloma virus high risk (HPV-HR) Negative for intraepithelial lesion (NILM) Computer applications to medicine. Medical informatics Gabriela CARACOSTEA verfasserin aut Cristina BUTUZA verfasserin aut Radu POPP verfasserin aut Daniel MUREŞAN verfasserin aut Sorana D. BOLBOACĂ verfasserin aut Ioana ROTAR verfasserin aut Felicia PETRIŞOR verfasserin aut Marius FĂRCAŞ verfasserin aut In Applied Medical Informatics Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, 2009 27(2010), 3, Seite 43-52 (DE-627)629833281 (DE-600)2558685-3 20677855 nnns volume:27 year:2010 number:3 pages:43-52 https://doaj.org/article/1bfb35e137d3452298e3a8af40399f76 kostenfrei http://ami.info.umfcluj.ro/Full-text/AMI_27_2010/AMI(27)_043_052.pdf kostenfrei https://doaj.org/toc/1224-5593 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 27 2010 3 43-52 |
allfields_unstemmed |
(DE-627)DOAJ079490379 (DE-599)DOAJ1bfb35e137d3452298e3a8af40399f76 DE-627 ger DE-627 rakwb eng R858-859.7 Florin STAMATIAN verfasserin aut The Analysis of Genetic Polymorphism. The Relationship between Interleukin – 4 Polymorphisms and Intraepithelial Cervical Neoplasia 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives: Interleukin 4 plays a critical role in T helper 2 responses to HPV infection and angiogenesis. The present study aim to study the association between the IL4 promoter polymorphism – 590 C<T, respectively VNTR intron 2 polymorphism and cervical intraepithelial neoplasia. Material and method: We have realized a prospective case controls study that included 128 cases of intraepithelial neoplasia positive for HPV HR testing and 111 controls negative for intraepithelial lesion and also negative for HPV HR. Clinical examination was performed on each patient; blood and cervical sample were obtained. Cervical probes were analyzed regarding cytology and HPV HR testing. From peripheral blood DNA sample was obtain followed by genotype analysis for IL4 -590 C<T using PCR RFLP, respectively IL4 70 bp VNTR determined by PCR. Results: The absolute frequency of genotypes for IL4 -590 C<T was T/T-5, C/T-42, C/C-81 in the cases group respectively T/T-2, C/T-32, C/C-77 in the control group. The chi-square test had a value of 0.983 (p=0.321) while considering the presence of a minimum one single variant allele as a risk factor for cervical cancer, respectively 0.926 (p=0.336) for homozygous variant genotype. Odds ratio was 0.761 (95%CI [0.443-1.306]) while considering C/T+T/T respectively 2R/3R, 2R/2R as a risk factor, and 0.451 (95%CI 95% [0.086-2.374]) - TT respectively 2R/2R as a risk factor. Conclusion: No linear statistical significant association has been found between IL4 polymorphism and cervical neoplasia (p = 0.322). Genetic polymorphism Restriction fragment length polymorphism (RFLP) Polymerase chain reaction (PCR) Interleukin-4 (IL4) Cervical intraepithelial neoplasia (CIN) Human papiloma virus (HPV) infection Human papiloma virus high risk (HPV-HR) Negative for intraepithelial lesion (NILM) Computer applications to medicine. Medical informatics Gabriela CARACOSTEA verfasserin aut Cristina BUTUZA verfasserin aut Radu POPP verfasserin aut Daniel MUREŞAN verfasserin aut Sorana D. BOLBOACĂ verfasserin aut Ioana ROTAR verfasserin aut Felicia PETRIŞOR verfasserin aut Marius FĂRCAŞ verfasserin aut In Applied Medical Informatics Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, 2009 27(2010), 3, Seite 43-52 (DE-627)629833281 (DE-600)2558685-3 20677855 nnns volume:27 year:2010 number:3 pages:43-52 https://doaj.org/article/1bfb35e137d3452298e3a8af40399f76 kostenfrei http://ami.info.umfcluj.ro/Full-text/AMI_27_2010/AMI(27)_043_052.pdf kostenfrei https://doaj.org/toc/1224-5593 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 27 2010 3 43-52 |
allfieldsGer |
(DE-627)DOAJ079490379 (DE-599)DOAJ1bfb35e137d3452298e3a8af40399f76 DE-627 ger DE-627 rakwb eng R858-859.7 Florin STAMATIAN verfasserin aut The Analysis of Genetic Polymorphism. The Relationship between Interleukin – 4 Polymorphisms and Intraepithelial Cervical Neoplasia 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives: Interleukin 4 plays a critical role in T helper 2 responses to HPV infection and angiogenesis. The present study aim to study the association between the IL4 promoter polymorphism – 590 C<T, respectively VNTR intron 2 polymorphism and cervical intraepithelial neoplasia. Material and method: We have realized a prospective case controls study that included 128 cases of intraepithelial neoplasia positive for HPV HR testing and 111 controls negative for intraepithelial lesion and also negative for HPV HR. Clinical examination was performed on each patient; blood and cervical sample were obtained. Cervical probes were analyzed regarding cytology and HPV HR testing. From peripheral blood DNA sample was obtain followed by genotype analysis for IL4 -590 C<T using PCR RFLP, respectively IL4 70 bp VNTR determined by PCR. Results: The absolute frequency of genotypes for IL4 -590 C<T was T/T-5, C/T-42, C/C-81 in the cases group respectively T/T-2, C/T-32, C/C-77 in the control group. The chi-square test had a value of 0.983 (p=0.321) while considering the presence of a minimum one single variant allele as a risk factor for cervical cancer, respectively 0.926 (p=0.336) for homozygous variant genotype. Odds ratio was 0.761 (95%CI [0.443-1.306]) while considering C/T+T/T respectively 2R/3R, 2R/2R as a risk factor, and 0.451 (95%CI 95% [0.086-2.374]) - TT respectively 2R/2R as a risk factor. Conclusion: No linear statistical significant association has been found between IL4 polymorphism and cervical neoplasia (p = 0.322). Genetic polymorphism Restriction fragment length polymorphism (RFLP) Polymerase chain reaction (PCR) Interleukin-4 (IL4) Cervical intraepithelial neoplasia (CIN) Human papiloma virus (HPV) infection Human papiloma virus high risk (HPV-HR) Negative for intraepithelial lesion (NILM) Computer applications to medicine. Medical informatics Gabriela CARACOSTEA verfasserin aut Cristina BUTUZA verfasserin aut Radu POPP verfasserin aut Daniel MUREŞAN verfasserin aut Sorana D. BOLBOACĂ verfasserin aut Ioana ROTAR verfasserin aut Felicia PETRIŞOR verfasserin aut Marius FĂRCAŞ verfasserin aut In Applied Medical Informatics Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, 2009 27(2010), 3, Seite 43-52 (DE-627)629833281 (DE-600)2558685-3 20677855 nnns volume:27 year:2010 number:3 pages:43-52 https://doaj.org/article/1bfb35e137d3452298e3a8af40399f76 kostenfrei http://ami.info.umfcluj.ro/Full-text/AMI_27_2010/AMI(27)_043_052.pdf kostenfrei https://doaj.org/toc/1224-5593 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 27 2010 3 43-52 |
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(DE-627)DOAJ079490379 (DE-599)DOAJ1bfb35e137d3452298e3a8af40399f76 DE-627 ger DE-627 rakwb eng R858-859.7 Florin STAMATIAN verfasserin aut The Analysis of Genetic Polymorphism. The Relationship between Interleukin – 4 Polymorphisms and Intraepithelial Cervical Neoplasia 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives: Interleukin 4 plays a critical role in T helper 2 responses to HPV infection and angiogenesis. The present study aim to study the association between the IL4 promoter polymorphism – 590 C<T, respectively VNTR intron 2 polymorphism and cervical intraepithelial neoplasia. Material and method: We have realized a prospective case controls study that included 128 cases of intraepithelial neoplasia positive for HPV HR testing and 111 controls negative for intraepithelial lesion and also negative for HPV HR. Clinical examination was performed on each patient; blood and cervical sample were obtained. Cervical probes were analyzed regarding cytology and HPV HR testing. From peripheral blood DNA sample was obtain followed by genotype analysis for IL4 -590 C<T using PCR RFLP, respectively IL4 70 bp VNTR determined by PCR. Results: The absolute frequency of genotypes for IL4 -590 C<T was T/T-5, C/T-42, C/C-81 in the cases group respectively T/T-2, C/T-32, C/C-77 in the control group. The chi-square test had a value of 0.983 (p=0.321) while considering the presence of a minimum one single variant allele as a risk factor for cervical cancer, respectively 0.926 (p=0.336) for homozygous variant genotype. Odds ratio was 0.761 (95%CI [0.443-1.306]) while considering C/T+T/T respectively 2R/3R, 2R/2R as a risk factor, and 0.451 (95%CI 95% [0.086-2.374]) - TT respectively 2R/2R as a risk factor. Conclusion: No linear statistical significant association has been found between IL4 polymorphism and cervical neoplasia (p = 0.322). Genetic polymorphism Restriction fragment length polymorphism (RFLP) Polymerase chain reaction (PCR) Interleukin-4 (IL4) Cervical intraepithelial neoplasia (CIN) Human papiloma virus (HPV) infection Human papiloma virus high risk (HPV-HR) Negative for intraepithelial lesion (NILM) Computer applications to medicine. Medical informatics Gabriela CARACOSTEA verfasserin aut Cristina BUTUZA verfasserin aut Radu POPP verfasserin aut Daniel MUREŞAN verfasserin aut Sorana D. BOLBOACĂ verfasserin aut Ioana ROTAR verfasserin aut Felicia PETRIŞOR verfasserin aut Marius FĂRCAŞ verfasserin aut In Applied Medical Informatics Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, 2009 27(2010), 3, Seite 43-52 (DE-627)629833281 (DE-600)2558685-3 20677855 nnns volume:27 year:2010 number:3 pages:43-52 https://doaj.org/article/1bfb35e137d3452298e3a8af40399f76 kostenfrei http://ami.info.umfcluj.ro/Full-text/AMI_27_2010/AMI(27)_043_052.pdf kostenfrei https://doaj.org/toc/1224-5593 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 27 2010 3 43-52 |
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Genetic polymorphism Restriction fragment length polymorphism (RFLP) Polymerase chain reaction (PCR) Interleukin-4 (IL4) Cervical intraepithelial neoplasia (CIN) Human papiloma virus (HPV) infection Human papiloma virus high risk (HPV-HR) Negative for intraepithelial lesion (NILM) Computer applications to medicine. Medical informatics |
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Florin STAMATIAN @@aut@@ Gabriela CARACOSTEA @@aut@@ Cristina BUTUZA @@aut@@ Radu POPP @@aut@@ Daniel MUREŞAN @@aut@@ Sorana D. BOLBOACĂ @@aut@@ Ioana ROTAR @@aut@@ Felicia PETRIŞOR @@aut@@ Marius FĂRCAŞ @@aut@@ |
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Florin STAMATIAN misc R858-859.7 misc Genetic polymorphism misc Restriction fragment length polymorphism (RFLP) misc Polymerase chain reaction (PCR) misc Interleukin-4 (IL4) misc Cervical intraepithelial neoplasia (CIN) misc Human papiloma virus (HPV) infection misc Human papiloma virus high risk (HPV-HR) misc Negative for intraepithelial lesion (NILM) misc Computer applications to medicine. Medical informatics The Analysis of Genetic Polymorphism. The Relationship between Interleukin – 4 Polymorphisms and Intraepithelial Cervical Neoplasia |
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R858-859.7 The Analysis of Genetic Polymorphism. The Relationship between Interleukin – 4 Polymorphisms and Intraepithelial Cervical Neoplasia Genetic polymorphism Restriction fragment length polymorphism (RFLP) Polymerase chain reaction (PCR) Interleukin-4 (IL4) Cervical intraepithelial neoplasia (CIN) Human papiloma virus (HPV) infection Human papiloma virus high risk (HPV-HR) Negative for intraepithelial lesion (NILM) |
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misc R858-859.7 misc Genetic polymorphism misc Restriction fragment length polymorphism (RFLP) misc Polymerase chain reaction (PCR) misc Interleukin-4 (IL4) misc Cervical intraepithelial neoplasia (CIN) misc Human papiloma virus (HPV) infection misc Human papiloma virus high risk (HPV-HR) misc Negative for intraepithelial lesion (NILM) misc Computer applications to medicine. Medical informatics |
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The Analysis of Genetic Polymorphism. The Relationship between Interleukin – 4 Polymorphisms and Intraepithelial Cervical Neoplasia |
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Florin STAMATIAN |
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Applied Medical Informatics |
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Florin STAMATIAN Gabriela CARACOSTEA Cristina BUTUZA Radu POPP Daniel MUREŞAN Sorana D. BOLBOACĂ Ioana ROTAR Felicia PETRIŞOR Marius FĂRCAŞ |
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analysis of genetic polymorphism. the relationship between interleukin – 4 polymorphisms and intraepithelial cervical neoplasia |
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The Analysis of Genetic Polymorphism. The Relationship between Interleukin – 4 Polymorphisms and Intraepithelial Cervical Neoplasia |
abstract |
Objectives: Interleukin 4 plays a critical role in T helper 2 responses to HPV infection and angiogenesis. The present study aim to study the association between the IL4 promoter polymorphism – 590 C<T, respectively VNTR intron 2 polymorphism and cervical intraepithelial neoplasia. Material and method: We have realized a prospective case controls study that included 128 cases of intraepithelial neoplasia positive for HPV HR testing and 111 controls negative for intraepithelial lesion and also negative for HPV HR. Clinical examination was performed on each patient; blood and cervical sample were obtained. Cervical probes were analyzed regarding cytology and HPV HR testing. From peripheral blood DNA sample was obtain followed by genotype analysis for IL4 -590 C<T using PCR RFLP, respectively IL4 70 bp VNTR determined by PCR. Results: The absolute frequency of genotypes for IL4 -590 C<T was T/T-5, C/T-42, C/C-81 in the cases group respectively T/T-2, C/T-32, C/C-77 in the control group. The chi-square test had a value of 0.983 (p=0.321) while considering the presence of a minimum one single variant allele as a risk factor for cervical cancer, respectively 0.926 (p=0.336) for homozygous variant genotype. Odds ratio was 0.761 (95%CI [0.443-1.306]) while considering C/T+T/T respectively 2R/3R, 2R/2R as a risk factor, and 0.451 (95%CI 95% [0.086-2.374]) - TT respectively 2R/2R as a risk factor. Conclusion: No linear statistical significant association has been found between IL4 polymorphism and cervical neoplasia (p = 0.322). |
abstractGer |
Objectives: Interleukin 4 plays a critical role in T helper 2 responses to HPV infection and angiogenesis. The present study aim to study the association between the IL4 promoter polymorphism – 590 C<T, respectively VNTR intron 2 polymorphism and cervical intraepithelial neoplasia. Material and method: We have realized a prospective case controls study that included 128 cases of intraepithelial neoplasia positive for HPV HR testing and 111 controls negative for intraepithelial lesion and also negative for HPV HR. Clinical examination was performed on each patient; blood and cervical sample were obtained. Cervical probes were analyzed regarding cytology and HPV HR testing. From peripheral blood DNA sample was obtain followed by genotype analysis for IL4 -590 C<T using PCR RFLP, respectively IL4 70 bp VNTR determined by PCR. Results: The absolute frequency of genotypes for IL4 -590 C<T was T/T-5, C/T-42, C/C-81 in the cases group respectively T/T-2, C/T-32, C/C-77 in the control group. The chi-square test had a value of 0.983 (p=0.321) while considering the presence of a minimum one single variant allele as a risk factor for cervical cancer, respectively 0.926 (p=0.336) for homozygous variant genotype. Odds ratio was 0.761 (95%CI [0.443-1.306]) while considering C/T+T/T respectively 2R/3R, 2R/2R as a risk factor, and 0.451 (95%CI 95% [0.086-2.374]) - TT respectively 2R/2R as a risk factor. Conclusion: No linear statistical significant association has been found between IL4 polymorphism and cervical neoplasia (p = 0.322). |
abstract_unstemmed |
Objectives: Interleukin 4 plays a critical role in T helper 2 responses to HPV infection and angiogenesis. The present study aim to study the association between the IL4 promoter polymorphism – 590 C<T, respectively VNTR intron 2 polymorphism and cervical intraepithelial neoplasia. Material and method: We have realized a prospective case controls study that included 128 cases of intraepithelial neoplasia positive for HPV HR testing and 111 controls negative for intraepithelial lesion and also negative for HPV HR. Clinical examination was performed on each patient; blood and cervical sample were obtained. Cervical probes were analyzed regarding cytology and HPV HR testing. From peripheral blood DNA sample was obtain followed by genotype analysis for IL4 -590 C<T using PCR RFLP, respectively IL4 70 bp VNTR determined by PCR. Results: The absolute frequency of genotypes for IL4 -590 C<T was T/T-5, C/T-42, C/C-81 in the cases group respectively T/T-2, C/T-32, C/C-77 in the control group. The chi-square test had a value of 0.983 (p=0.321) while considering the presence of a minimum one single variant allele as a risk factor for cervical cancer, respectively 0.926 (p=0.336) for homozygous variant genotype. Odds ratio was 0.761 (95%CI [0.443-1.306]) while considering C/T+T/T respectively 2R/3R, 2R/2R as a risk factor, and 0.451 (95%CI 95% [0.086-2.374]) - TT respectively 2R/2R as a risk factor. Conclusion: No linear statistical significant association has been found between IL4 polymorphism and cervical neoplasia (p = 0.322). |
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The Analysis of Genetic Polymorphism. The Relationship between Interleukin – 4 Polymorphisms and Intraepithelial Cervical Neoplasia |
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https://doaj.org/article/1bfb35e137d3452298e3a8af40399f76 http://ami.info.umfcluj.ro/Full-text/AMI_27_2010/AMI(27)_043_052.pdf https://doaj.org/toc/1224-5593 |
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Gabriela CARACOSTEA Cristina BUTUZA Radu POPP Daniel MUREŞAN Sorana D. BOLBOACĂ Ioana ROTAR Felicia PETRIŞOR Marius FĂRCAŞ |
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