The Analysis of Genetic Polymorphism. The Relationship between Interleukin – 4 Polymorphisms and Intraepithelial Cervical Neoplasia

Objectives: Interleukin 4 plays a critical role in T helper 2 responses to HPV infection and angiogenesis. The present study aim to study the association between the IL4 promoter polymorphism – 590 C<T, respectively VNTR intron 2 polymorphism and cervical intraepithelial neoplasia. Material and m...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Florin STAMATIAN [verfasserIn] Gabriela CARACOSTEA [verfasserIn] Cristina BUTUZA [verfasserIn] Radu POPP [verfasserIn] Daniel MUREŞAN [verfasserIn] Sorana D. BOLBOACĂ [verfasserIn] Ioana ROTAR [verfasserIn] Felicia PETRIŞOR [verfasserIn] Marius FĂRCAŞ [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2010

Schlagwörter:	Genetic polymorphism Restriction fragment length polymorphism (RFLP) Polymerase chain reaction (PCR) Interleukin-4 (IL4) Cervical intraepithelial neoplasia (CIN) Human papiloma virus (HPV) infection Human papiloma virus high risk (HPV-HR) Negative for intraepithelial lesion (NILM)

Übergeordnetes Werk:	In: Applied Medical Informatics - Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, 2009, 27(2010), 3, Seite 43-52
Übergeordnetes Werk:	volume:27 ; year:2010 ; number:3 ; pages:43-52

Links:	Link aufrufen Link aufrufen Journal toc

Katalog-ID:	DOAJ079490379

Internformat


LEADER	01000naa a22002652 4500
001	DOAJ079490379
003	DE-627
005	20230307014623.0
007	cr uuu---uuuuu
008	230307s2010 xx \|\|\|\|\|o 00\| \|\|eng c
035			\|a (DE-627)DOAJ079490379
035			\|a (DE-599)DOAJ1bfb35e137d3452298e3a8af40399f76
040			\|a DE-627 \|b ger \|c DE-627 \|e rakwb
041			\|a eng
050		0	\|a R858-859.7
100	0		\|a Florin STAMATIAN \|e verfasserin \|4 aut
245	1	4	\|a The Analysis of Genetic Polymorphism. The Relationship between Interleukin – 4 Polymorphisms and Intraepithelial Cervical Neoplasia
264		1	\|c 2010
336			\|a Text \|b txt \|2 rdacontent
337			\|a Computermedien \|b c \|2 rdamedia
338			\|a Online-Ressource \|b cr \|2 rdacarrier
520			\|a Objectives: Interleukin 4 plays a critical role in T helper 2 responses to HPV infection and angiogenesis. The present study aim to study the association between the IL4 promoter polymorphism – 590 C<T, respectively VNTR intron 2 polymorphism and cervical intraepithelial neoplasia. Material and method: We have realized a prospective case controls study that included 128 cases of intraepithelial neoplasia positive for HPV HR testing and 111 controls negative for intraepithelial lesion and also negative for HPV HR. Clinical examination was performed on each patient; blood and cervical sample were obtained. Cervical probes were analyzed regarding cytology and HPV HR testing. From peripheral blood DNA sample was obtain followed by genotype analysis for IL4 -590 C<T using PCR RFLP, respectively IL4 70 bp VNTR determined by PCR. Results: The absolute frequency of genotypes for IL4 -590 C<T was T/T-5, C/T-42, C/C-81 in the cases group respectively T/T-2, C/T-32, C/C-77 in the control group. The chi-square test had a value of 0.983 (p=0.321) while considering the presence of a minimum one single variant allele as a risk factor for cervical cancer, respectively 0.926 (p=0.336) for homozygous variant genotype. Odds ratio was 0.761 (95%CI [0.443-1.306]) while considering C/T+T/T respectively 2R/3R, 2R/2R as a risk factor, and 0.451 (95%CI 95% [0.086-2.374]) - TT respectively 2R/2R as a risk factor. Conclusion: No linear statistical significant association has been found between IL4 polymorphism and cervical neoplasia (p = 0.322).
650		4	\|a Genetic polymorphism
650		4	\|a Restriction fragment length polymorphism (RFLP)
650		4	\|a Polymerase chain reaction (PCR)
650		4	\|a Interleukin-4 (IL4)
650		4	\|a Cervical intraepithelial neoplasia (CIN)
650		4	\|a Human papiloma virus (HPV) infection
650		4	\|a Human papiloma virus high risk (HPV-HR)
650		4	\|a Negative for intraepithelial lesion (NILM)
653		0	\|a Computer applications to medicine. Medical informatics
700	0		\|a Gabriela CARACOSTEA \|e verfasserin \|4 aut
700	0		\|a Cristina BUTUZA \|e verfasserin \|4 aut
700	0		\|a Radu POPP \|e verfasserin \|4 aut
700	0		\|a Daniel MUREŞAN \|e verfasserin \|4 aut
700	0		\|a Sorana D. BOLBOACĂ \|e verfasserin \|4 aut
700	0		\|a Ioana ROTAR \|e verfasserin \|4 aut
700	0		\|a Felicia PETRIŞOR \|e verfasserin \|4 aut
700	0		\|a Marius FĂRCAŞ \|e verfasserin \|4 aut
773	0	8	\|i In \|t Applied Medical Informatics \|d Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, 2009 \|g 27(2010), 3, Seite 43-52 \|w (DE-627)629833281 \|w (DE-600)2558685-3 \|x 20677855 \|7 nnns
773	1	8	\|g volume:27 \|g year:2010 \|g number:3 \|g pages:43-52
856	4	0	\|u https://doaj.org/article/1bfb35e137d3452298e3a8af40399f76 \|z kostenfrei
856	4	0	\|u http://ami.info.umfcluj.ro/Full-text/AMI_27_2010/AMI(27)_043_052.pdf \|z kostenfrei
856	4	2	\|u https://doaj.org/toc/1224-5593 \|y Journal toc \|z kostenfrei
912			\|a GBV_USEFLAG_A
912			\|a SYSFLAG_A
912			\|a GBV_DOAJ
912			\|a GBV_ILN_20
912			\|a GBV_ILN_22
912			\|a GBV_ILN_23
912			\|a GBV_ILN_24
912			\|a GBV_ILN_31
912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_95
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_151
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_206
912			\|a GBV_ILN_213
912			\|a GBV_ILN_230
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_602
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4367
912			\|a GBV_ILN_4700
951			\|a AR
952			\|d 27 \|j 2010 \|e 3 \|h 43-52

Indexfelder

author_variant	f s fs g c gc c b cb r p rp d m dm s d b sdb i r ir f p fp m f mf
matchkey_str	article:20677855:2010----::haayiogntcoyopimhrltosibtennelui4oyopimadn
hierarchy_sort_str	2010
callnumber-subject-code	R
publishDate	2010
allfields	(DE-627)DOAJ079490379 (DE-599)DOAJ1bfb35e137d3452298e3a8af40399f76 DE-627 ger DE-627 rakwb eng R858-859.7 Florin STAMATIAN verfasserin aut The Analysis of Genetic Polymorphism. The Relationship between Interleukin – 4 Polymorphisms and Intraepithelial Cervical Neoplasia 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives: Interleukin 4 plays a critical role in T helper 2 responses to HPV infection and angiogenesis. The present study aim to study the association between the IL4 promoter polymorphism – 590 C<T, respectively VNTR intron 2 polymorphism and cervical intraepithelial neoplasia. Material and method: We have realized a prospective case controls study that included 128 cases of intraepithelial neoplasia positive for HPV HR testing and 111 controls negative for intraepithelial lesion and also negative for HPV HR. Clinical examination was performed on each patient; blood and cervical sample were obtained. Cervical probes were analyzed regarding cytology and HPV HR testing. From peripheral blood DNA sample was obtain followed by genotype analysis for IL4 -590 C<T using PCR RFLP, respectively IL4 70 bp VNTR determined by PCR. Results: The absolute frequency of genotypes for IL4 -590 C<T was T/T-5, C/T-42, C/C-81 in the cases group respectively T/T-2, C/T-32, C/C-77 in the control group. The chi-square test had a value of 0.983 (p=0.321) while considering the presence of a minimum one single variant allele as a risk factor for cervical cancer, respectively 0.926 (p=0.336) for homozygous variant genotype. Odds ratio was 0.761 (95%CI [0.443-1.306]) while considering C/T+T/T respectively 2R/3R, 2R/2R as a risk factor, and 0.451 (95%CI 95% [0.086-2.374]) - TT respectively 2R/2R as a risk factor. Conclusion: No linear statistical significant association has been found between IL4 polymorphism and cervical neoplasia (p = 0.322). Genetic polymorphism Restriction fragment length polymorphism (RFLP) Polymerase chain reaction (PCR) Interleukin-4 (IL4) Cervical intraepithelial neoplasia (CIN) Human papiloma virus (HPV) infection Human papiloma virus high risk (HPV-HR) Negative for intraepithelial lesion (NILM) Computer applications to medicine. Medical informatics Gabriela CARACOSTEA verfasserin aut Cristina BUTUZA verfasserin aut Radu POPP verfasserin aut Daniel MUREŞAN verfasserin aut Sorana D. BOLBOACĂ verfasserin aut Ioana ROTAR verfasserin aut Felicia PETRIŞOR verfasserin aut Marius FĂRCAŞ verfasserin aut In Applied Medical Informatics Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, 2009 27(2010), 3, Seite 43-52 (DE-627)629833281 (DE-600)2558685-3 20677855 nnns volume:27 year:2010 number:3 pages:43-52 https://doaj.org/article/1bfb35e137d3452298e3a8af40399f76 kostenfrei http://ami.info.umfcluj.ro/Full-text/AMI_27_2010/AMI(27)_043_052.pdf kostenfrei https://doaj.org/toc/1224-5593 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 27 2010 3 43-52
spelling	(DE-627)DOAJ079490379 (DE-599)DOAJ1bfb35e137d3452298e3a8af40399f76 DE-627 ger DE-627 rakwb eng R858-859.7 Florin STAMATIAN verfasserin aut The Analysis of Genetic Polymorphism. The Relationship between Interleukin – 4 Polymorphisms and Intraepithelial Cervical Neoplasia 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives: Interleukin 4 plays a critical role in T helper 2 responses to HPV infection and angiogenesis. The present study aim to study the association between the IL4 promoter polymorphism – 590 C<T, respectively VNTR intron 2 polymorphism and cervical intraepithelial neoplasia. Material and method: We have realized a prospective case controls study that included 128 cases of intraepithelial neoplasia positive for HPV HR testing and 111 controls negative for intraepithelial lesion and also negative for HPV HR. Clinical examination was performed on each patient; blood and cervical sample were obtained. Cervical probes were analyzed regarding cytology and HPV HR testing. From peripheral blood DNA sample was obtain followed by genotype analysis for IL4 -590 C<T using PCR RFLP, respectively IL4 70 bp VNTR determined by PCR. Results: The absolute frequency of genotypes for IL4 -590 C<T was T/T-5, C/T-42, C/C-81 in the cases group respectively T/T-2, C/T-32, C/C-77 in the control group. The chi-square test had a value of 0.983 (p=0.321) while considering the presence of a minimum one single variant allele as a risk factor for cervical cancer, respectively 0.926 (p=0.336) for homozygous variant genotype. Odds ratio was 0.761 (95%CI [0.443-1.306]) while considering C/T+T/T respectively 2R/3R, 2R/2R as a risk factor, and 0.451 (95%CI 95% [0.086-2.374]) - TT respectively 2R/2R as a risk factor. Conclusion: No linear statistical significant association has been found between IL4 polymorphism and cervical neoplasia (p = 0.322). Genetic polymorphism Restriction fragment length polymorphism (RFLP) Polymerase chain reaction (PCR) Interleukin-4 (IL4) Cervical intraepithelial neoplasia (CIN) Human papiloma virus (HPV) infection Human papiloma virus high risk (HPV-HR) Negative for intraepithelial lesion (NILM) Computer applications to medicine. Medical informatics Gabriela CARACOSTEA verfasserin aut Cristina BUTUZA verfasserin aut Radu POPP verfasserin aut Daniel MUREŞAN verfasserin aut Sorana D. BOLBOACĂ verfasserin aut Ioana ROTAR verfasserin aut Felicia PETRIŞOR verfasserin aut Marius FĂRCAŞ verfasserin aut In Applied Medical Informatics Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, 2009 27(2010), 3, Seite 43-52 (DE-627)629833281 (DE-600)2558685-3 20677855 nnns volume:27 year:2010 number:3 pages:43-52 https://doaj.org/article/1bfb35e137d3452298e3a8af40399f76 kostenfrei http://ami.info.umfcluj.ro/Full-text/AMI_27_2010/AMI(27)_043_052.pdf kostenfrei https://doaj.org/toc/1224-5593 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 27 2010 3 43-52
allfields_unstemmed	(DE-627)DOAJ079490379 (DE-599)DOAJ1bfb35e137d3452298e3a8af40399f76 DE-627 ger DE-627 rakwb eng R858-859.7 Florin STAMATIAN verfasserin aut The Analysis of Genetic Polymorphism. The Relationship between Interleukin – 4 Polymorphisms and Intraepithelial Cervical Neoplasia 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives: Interleukin 4 plays a critical role in T helper 2 responses to HPV infection and angiogenesis. The present study aim to study the association between the IL4 promoter polymorphism – 590 C<T, respectively VNTR intron 2 polymorphism and cervical intraepithelial neoplasia. Material and method: We have realized a prospective case controls study that included 128 cases of intraepithelial neoplasia positive for HPV HR testing and 111 controls negative for intraepithelial lesion and also negative for HPV HR. Clinical examination was performed on each patient; blood and cervical sample were obtained. Cervical probes were analyzed regarding cytology and HPV HR testing. From peripheral blood DNA sample was obtain followed by genotype analysis for IL4 -590 C<T using PCR RFLP, respectively IL4 70 bp VNTR determined by PCR. Results: The absolute frequency of genotypes for IL4 -590 C<T was T/T-5, C/T-42, C/C-81 in the cases group respectively T/T-2, C/T-32, C/C-77 in the control group. The chi-square test had a value of 0.983 (p=0.321) while considering the presence of a minimum one single variant allele as a risk factor for cervical cancer, respectively 0.926 (p=0.336) for homozygous variant genotype. Odds ratio was 0.761 (95%CI [0.443-1.306]) while considering C/T+T/T respectively 2R/3R, 2R/2R as a risk factor, and 0.451 (95%CI 95% [0.086-2.374]) - TT respectively 2R/2R as a risk factor. Conclusion: No linear statistical significant association has been found between IL4 polymorphism and cervical neoplasia (p = 0.322). Genetic polymorphism Restriction fragment length polymorphism (RFLP) Polymerase chain reaction (PCR) Interleukin-4 (IL4) Cervical intraepithelial neoplasia (CIN) Human papiloma virus (HPV) infection Human papiloma virus high risk (HPV-HR) Negative for intraepithelial lesion (NILM) Computer applications to medicine. Medical informatics Gabriela CARACOSTEA verfasserin aut Cristina BUTUZA verfasserin aut Radu POPP verfasserin aut Daniel MUREŞAN verfasserin aut Sorana D. BOLBOACĂ verfasserin aut Ioana ROTAR verfasserin aut Felicia PETRIŞOR verfasserin aut Marius FĂRCAŞ verfasserin aut In Applied Medical Informatics Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, 2009 27(2010), 3, Seite 43-52 (DE-627)629833281 (DE-600)2558685-3 20677855 nnns volume:27 year:2010 number:3 pages:43-52 https://doaj.org/article/1bfb35e137d3452298e3a8af40399f76 kostenfrei http://ami.info.umfcluj.ro/Full-text/AMI_27_2010/AMI(27)_043_052.pdf kostenfrei https://doaj.org/toc/1224-5593 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 27 2010 3 43-52
allfieldsGer	(DE-627)DOAJ079490379 (DE-599)DOAJ1bfb35e137d3452298e3a8af40399f76 DE-627 ger DE-627 rakwb eng R858-859.7 Florin STAMATIAN verfasserin aut The Analysis of Genetic Polymorphism. The Relationship between Interleukin – 4 Polymorphisms and Intraepithelial Cervical Neoplasia 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives: Interleukin 4 plays a critical role in T helper 2 responses to HPV infection and angiogenesis. The present study aim to study the association between the IL4 promoter polymorphism – 590 C<T, respectively VNTR intron 2 polymorphism and cervical intraepithelial neoplasia. Material and method: We have realized a prospective case controls study that included 128 cases of intraepithelial neoplasia positive for HPV HR testing and 111 controls negative for intraepithelial lesion and also negative for HPV HR. Clinical examination was performed on each patient; blood and cervical sample were obtained. Cervical probes were analyzed regarding cytology and HPV HR testing. From peripheral blood DNA sample was obtain followed by genotype analysis for IL4 -590 C<T using PCR RFLP, respectively IL4 70 bp VNTR determined by PCR. Results: The absolute frequency of genotypes for IL4 -590 C<T was T/T-5, C/T-42, C/C-81 in the cases group respectively T/T-2, C/T-32, C/C-77 in the control group. The chi-square test had a value of 0.983 (p=0.321) while considering the presence of a minimum one single variant allele as a risk factor for cervical cancer, respectively 0.926 (p=0.336) for homozygous variant genotype. Odds ratio was 0.761 (95%CI [0.443-1.306]) while considering C/T+T/T respectively 2R/3R, 2R/2R as a risk factor, and 0.451 (95%CI 95% [0.086-2.374]) - TT respectively 2R/2R as a risk factor. Conclusion: No linear statistical significant association has been found between IL4 polymorphism and cervical neoplasia (p = 0.322). Genetic polymorphism Restriction fragment length polymorphism (RFLP) Polymerase chain reaction (PCR) Interleukin-4 (IL4) Cervical intraepithelial neoplasia (CIN) Human papiloma virus (HPV) infection Human papiloma virus high risk (HPV-HR) Negative for intraepithelial lesion (NILM) Computer applications to medicine. Medical informatics Gabriela CARACOSTEA verfasserin aut Cristina BUTUZA verfasserin aut Radu POPP verfasserin aut Daniel MUREŞAN verfasserin aut Sorana D. BOLBOACĂ verfasserin aut Ioana ROTAR verfasserin aut Felicia PETRIŞOR verfasserin aut Marius FĂRCAŞ verfasserin aut In Applied Medical Informatics Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, 2009 27(2010), 3, Seite 43-52 (DE-627)629833281 (DE-600)2558685-3 20677855 nnns volume:27 year:2010 number:3 pages:43-52 https://doaj.org/article/1bfb35e137d3452298e3a8af40399f76 kostenfrei http://ami.info.umfcluj.ro/Full-text/AMI_27_2010/AMI(27)_043_052.pdf kostenfrei https://doaj.org/toc/1224-5593 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 27 2010 3 43-52
allfieldsSound	(DE-627)DOAJ079490379 (DE-599)DOAJ1bfb35e137d3452298e3a8af40399f76 DE-627 ger DE-627 rakwb eng R858-859.7 Florin STAMATIAN verfasserin aut The Analysis of Genetic Polymorphism. The Relationship between Interleukin – 4 Polymorphisms and Intraepithelial Cervical Neoplasia 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives: Interleukin 4 plays a critical role in T helper 2 responses to HPV infection and angiogenesis. The present study aim to study the association between the IL4 promoter polymorphism – 590 C<T, respectively VNTR intron 2 polymorphism and cervical intraepithelial neoplasia. Material and method: We have realized a prospective case controls study that included 128 cases of intraepithelial neoplasia positive for HPV HR testing and 111 controls negative for intraepithelial lesion and also negative for HPV HR. Clinical examination was performed on each patient; blood and cervical sample were obtained. Cervical probes were analyzed regarding cytology and HPV HR testing. From peripheral blood DNA sample was obtain followed by genotype analysis for IL4 -590 C<T using PCR RFLP, respectively IL4 70 bp VNTR determined by PCR. Results: The absolute frequency of genotypes for IL4 -590 C<T was T/T-5, C/T-42, C/C-81 in the cases group respectively T/T-2, C/T-32, C/C-77 in the control group. The chi-square test had a value of 0.983 (p=0.321) while considering the presence of a minimum one single variant allele as a risk factor for cervical cancer, respectively 0.926 (p=0.336) for homozygous variant genotype. Odds ratio was 0.761 (95%CI [0.443-1.306]) while considering C/T+T/T respectively 2R/3R, 2R/2R as a risk factor, and 0.451 (95%CI 95% [0.086-2.374]) - TT respectively 2R/2R as a risk factor. Conclusion: No linear statistical significant association has been found between IL4 polymorphism and cervical neoplasia (p = 0.322). Genetic polymorphism Restriction fragment length polymorphism (RFLP) Polymerase chain reaction (PCR) Interleukin-4 (IL4) Cervical intraepithelial neoplasia (CIN) Human papiloma virus (HPV) infection Human papiloma virus high risk (HPV-HR) Negative for intraepithelial lesion (NILM) Computer applications to medicine. Medical informatics Gabriela CARACOSTEA verfasserin aut Cristina BUTUZA verfasserin aut Radu POPP verfasserin aut Daniel MUREŞAN verfasserin aut Sorana D. BOLBOACĂ verfasserin aut Ioana ROTAR verfasserin aut Felicia PETRIŞOR verfasserin aut Marius FĂRCAŞ verfasserin aut In Applied Medical Informatics Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, 2009 27(2010), 3, Seite 43-52 (DE-627)629833281 (DE-600)2558685-3 20677855 nnns volume:27 year:2010 number:3 pages:43-52 https://doaj.org/article/1bfb35e137d3452298e3a8af40399f76 kostenfrei http://ami.info.umfcluj.ro/Full-text/AMI_27_2010/AMI(27)_043_052.pdf kostenfrei https://doaj.org/toc/1224-5593 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 27 2010 3 43-52
language	English
source	In Applied Medical Informatics 27(2010), 3, Seite 43-52 volume:27 year:2010 number:3 pages:43-52
sourceStr	In Applied Medical Informatics 27(2010), 3, Seite 43-52 volume:27 year:2010 number:3 pages:43-52
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Genetic polymorphism Restriction fragment length polymorphism (RFLP) Polymerase chain reaction (PCR) Interleukin-4 (IL4) Cervical intraepithelial neoplasia (CIN) Human papiloma virus (HPV) infection Human papiloma virus high risk (HPV-HR) Negative for intraepithelial lesion (NILM) Computer applications to medicine. Medical informatics
isfreeaccess_bool	true
container_title	Applied Medical Informatics
authorswithroles_txt_mv	Florin STAMATIAN @@aut@@ Gabriela CARACOSTEA @@aut@@ Cristina BUTUZA @@aut@@ Radu POPP @@aut@@ Daniel MUREŞAN @@aut@@ Sorana D. BOLBOACĂ @@aut@@ Ioana ROTAR @@aut@@ Felicia PETRIŞOR @@aut@@ Marius FĂRCAŞ @@aut@@
publishDateDaySort_date	2010-01-01T00:00:00Z
hierarchy_top_id	629833281
id	DOAJ079490379
language_de	englisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000naa a22002652 4500</leader><controlfield tag="001">DOAJ079490379</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230307014623.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230307s2010 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ079490379</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ1bfb35e137d3452298e3a8af40399f76</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">R858-859.7</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Florin STAMATIAN</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="4"><subfield code="a">The Analysis of Genetic Polymorphism. The Relationship between Interleukin – 4 Polymorphisms and Intraepithelial Cervical Neoplasia</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2010</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Objectives: Interleukin 4 plays a critical role in T helper 2 responses to HPV infection and angiogenesis. The present study aim to study the association between the IL4 promoter polymorphism – 590 C<T, respectively VNTR intron 2 polymorphism and cervical intraepithelial neoplasia. Material and method: We have realized a prospective case controls study that included 128 cases of intraepithelial neoplasia positive for HPV HR testing and 111 controls negative for intraepithelial lesion and also negative for HPV HR. Clinical examination was performed on each patient; blood and cervical sample were obtained. Cervical probes were analyzed regarding cytology and HPV HR testing. From peripheral blood DNA sample was obtain followed by genotype analysis for IL4 -590 C<T using PCR RFLP, respectively IL4 70 bp VNTR determined by PCR. Results: The absolute frequency of genotypes for IL4 -590 C<T was T/T-5, C/T-42, C/C-81 in the cases group respectively T/T-2, C/T-32, C/C-77 in the control group. The chi-square test had a value of 0.983 (p=0.321) while considering the presence of a minimum one single variant allele as a risk factor for cervical cancer, respectively 0.926 (p=0.336) for homozygous variant genotype. Odds ratio was 0.761 (95%CI [0.443-1.306]) while considering C/T+T/T respectively 2R/3R, 2R/2R as a risk factor, and 0.451 (95%CI 95% [0.086-2.374]) - TT respectively 2R/2R as a risk factor. Conclusion: No linear statistical significant association has been found between IL4 polymorphism and cervical neoplasia (p = 0.322).</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Genetic polymorphism</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Restriction fragment length polymorphism (RFLP)</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Polymerase chain reaction (PCR)</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Interleukin-4 (IL4)</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cervical intraepithelial neoplasia (CIN)</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Human papiloma virus (HPV) infection</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Human papiloma virus high risk (HPV-HR)</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Negative for intraepithelial lesion (NILM)</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Computer applications to medicine. Medical informatics</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Gabriela CARACOSTEA</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Cristina BUTUZA</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Radu POPP</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Daniel MUREŞAN</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Sorana D. BOLBOACĂ</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Ioana ROTAR</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Felicia PETRIŞOR</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Marius FĂRCAŞ</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Applied Medical Informatics</subfield><subfield code="d">Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, 2009</subfield><subfield code="g">27(2010), 3, Seite 43-52</subfield><subfield code="w">(DE-627)629833281</subfield><subfield code="w">(DE-600)2558685-3</subfield><subfield code="x">20677855</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:27</subfield><subfield code="g">year:2010</subfield><subfield code="g">number:3</subfield><subfield code="g">pages:43-52</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/1bfb35e137d3452298e3a8af40399f76</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://ami.info.umfcluj.ro/Full-text/AMI_27_2010/AMI(27)_043_052.pdf</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/1224-5593</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_206</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2005</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2009</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2011</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2055</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2111</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">27</subfield><subfield code="j">2010</subfield><subfield code="e">3</subfield><subfield code="h">43-52</subfield></datafield></record></collection>
callnumber-first	R - Medicine
author	Florin STAMATIAN
spellingShingle	Florin STAMATIAN misc R858-859.7 misc Genetic polymorphism misc Restriction fragment length polymorphism (RFLP) misc Polymerase chain reaction (PCR) misc Interleukin-4 (IL4) misc Cervical intraepithelial neoplasia (CIN) misc Human papiloma virus (HPV) infection misc Human papiloma virus high risk (HPV-HR) misc Negative for intraepithelial lesion (NILM) misc Computer applications to medicine. Medical informatics The Analysis of Genetic Polymorphism. The Relationship between Interleukin – 4 Polymorphisms and Intraepithelial Cervical Neoplasia
authorStr	Florin STAMATIAN
ppnlink_with_tag_str_mv	@@773@@(DE-627)629833281
format	electronic Article
delete_txt_mv	keep
author_role	aut aut aut aut aut aut aut aut aut
collection	DOAJ
remote_str	true
callnumber-label	R858-859
illustrated	Not Illustrated
issn	20677855
topic_title	R858-859.7 The Analysis of Genetic Polymorphism. The Relationship between Interleukin – 4 Polymorphisms and Intraepithelial Cervical Neoplasia Genetic polymorphism Restriction fragment length polymorphism (RFLP) Polymerase chain reaction (PCR) Interleukin-4 (IL4) Cervical intraepithelial neoplasia (CIN) Human papiloma virus (HPV) infection Human papiloma virus high risk (HPV-HR) Negative for intraepithelial lesion (NILM)
topic	misc R858-859.7 misc Genetic polymorphism misc Restriction fragment length polymorphism (RFLP) misc Polymerase chain reaction (PCR) misc Interleukin-4 (IL4) misc Cervical intraepithelial neoplasia (CIN) misc Human papiloma virus (HPV) infection misc Human papiloma virus high risk (HPV-HR) misc Negative for intraepithelial lesion (NILM) misc Computer applications to medicine. Medical informatics
topic_unstemmed	misc R858-859.7 misc Genetic polymorphism misc Restriction fragment length polymorphism (RFLP) misc Polymerase chain reaction (PCR) misc Interleukin-4 (IL4) misc Cervical intraepithelial neoplasia (CIN) misc Human papiloma virus (HPV) infection misc Human papiloma virus high risk (HPV-HR) misc Negative for intraepithelial lesion (NILM) misc Computer applications to medicine. Medical informatics
topic_browse	misc R858-859.7 misc Genetic polymorphism misc Restriction fragment length polymorphism (RFLP) misc Polymerase chain reaction (PCR) misc Interleukin-4 (IL4) misc Cervical intraepithelial neoplasia (CIN) misc Human papiloma virus (HPV) infection misc Human papiloma virus high risk (HPV-HR) misc Negative for intraepithelial lesion (NILM) misc Computer applications to medicine. Medical informatics
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
format_main_str_mv	Text Zeitschrift/Artikel
carriertype_str_mv	cr
hierarchy_parent_title	Applied Medical Informatics
hierarchy_parent_id	629833281
hierarchy_top_title	Applied Medical Informatics
isfreeaccess_txt	true
familylinks_str_mv	(DE-627)629833281 (DE-600)2558685-3
title	The Analysis of Genetic Polymorphism. The Relationship between Interleukin – 4 Polymorphisms and Intraepithelial Cervical Neoplasia
ctrlnum	(DE-627)DOAJ079490379 (DE-599)DOAJ1bfb35e137d3452298e3a8af40399f76
title_full	The Analysis of Genetic Polymorphism. The Relationship between Interleukin – 4 Polymorphisms and Intraepithelial Cervical Neoplasia
author_sort	Florin STAMATIAN
journal	Applied Medical Informatics
journalStr	Applied Medical Informatics
callnumber-first-code	R
lang_code	eng
isOA_bool	true
recordtype	marc
publishDateSort	2010
contenttype_str_mv	txt
container_start_page	43
author_browse	Florin STAMATIAN Gabriela CARACOSTEA Cristina BUTUZA Radu POPP Daniel MUREŞAN Sorana D. BOLBOACĂ Ioana ROTAR Felicia PETRIŞOR Marius FĂRCAŞ
container_volume	27
class	R858-859.7
format_se	Elektronische Aufsätze
author-letter	Florin STAMATIAN
author2-role	verfasserin
title_sort	analysis of genetic polymorphism. the relationship between interleukin – 4 polymorphisms and intraepithelial cervical neoplasia
callnumber	R858-859.7
title_auth	The Analysis of Genetic Polymorphism. The Relationship between Interleukin – 4 Polymorphisms and Intraepithelial Cervical Neoplasia
abstract	Objectives: Interleukin 4 plays a critical role in T helper 2 responses to HPV infection and angiogenesis. The present study aim to study the association between the IL4 promoter polymorphism – 590 C<T, respectively VNTR intron 2 polymorphism and cervical intraepithelial neoplasia. Material and method: We have realized a prospective case controls study that included 128 cases of intraepithelial neoplasia positive for HPV HR testing and 111 controls negative for intraepithelial lesion and also negative for HPV HR. Clinical examination was performed on each patient; blood and cervical sample were obtained. Cervical probes were analyzed regarding cytology and HPV HR testing. From peripheral blood DNA sample was obtain followed by genotype analysis for IL4 -590 C<T using PCR RFLP, respectively IL4 70 bp VNTR determined by PCR. Results: The absolute frequency of genotypes for IL4 -590 C<T was T/T-5, C/T-42, C/C-81 in the cases group respectively T/T-2, C/T-32, C/C-77 in the control group. The chi-square test had a value of 0.983 (p=0.321) while considering the presence of a minimum one single variant allele as a risk factor for cervical cancer, respectively 0.926 (p=0.336) for homozygous variant genotype. Odds ratio was 0.761 (95%CI [0.443-1.306]) while considering C/T+T/T respectively 2R/3R, 2R/2R as a risk factor, and 0.451 (95%CI 95% [0.086-2.374]) - TT respectively 2R/2R as a risk factor. Conclusion: No linear statistical significant association has been found between IL4 polymorphism and cervical neoplasia (p = 0.322).
abstractGer	Objectives: Interleukin 4 plays a critical role in T helper 2 responses to HPV infection and angiogenesis. The present study aim to study the association between the IL4 promoter polymorphism – 590 C<T, respectively VNTR intron 2 polymorphism and cervical intraepithelial neoplasia. Material and method: We have realized a prospective case controls study that included 128 cases of intraepithelial neoplasia positive for HPV HR testing and 111 controls negative for intraepithelial lesion and also negative for HPV HR. Clinical examination was performed on each patient; blood and cervical sample were obtained. Cervical probes were analyzed regarding cytology and HPV HR testing. From peripheral blood DNA sample was obtain followed by genotype analysis for IL4 -590 C<T using PCR RFLP, respectively IL4 70 bp VNTR determined by PCR. Results: The absolute frequency of genotypes for IL4 -590 C<T was T/T-5, C/T-42, C/C-81 in the cases group respectively T/T-2, C/T-32, C/C-77 in the control group. The chi-square test had a value of 0.983 (p=0.321) while considering the presence of a minimum one single variant allele as a risk factor for cervical cancer, respectively 0.926 (p=0.336) for homozygous variant genotype. Odds ratio was 0.761 (95%CI [0.443-1.306]) while considering C/T+T/T respectively 2R/3R, 2R/2R as a risk factor, and 0.451 (95%CI 95% [0.086-2.374]) - TT respectively 2R/2R as a risk factor. Conclusion: No linear statistical significant association has been found between IL4 polymorphism and cervical neoplasia (p = 0.322).
abstract_unstemmed	Objectives: Interleukin 4 plays a critical role in T helper 2 responses to HPV infection and angiogenesis. The present study aim to study the association between the IL4 promoter polymorphism – 590 C<T, respectively VNTR intron 2 polymorphism and cervical intraepithelial neoplasia. Material and method: We have realized a prospective case controls study that included 128 cases of intraepithelial neoplasia positive for HPV HR testing and 111 controls negative for intraepithelial lesion and also negative for HPV HR. Clinical examination was performed on each patient; blood and cervical sample were obtained. Cervical probes were analyzed regarding cytology and HPV HR testing. From peripheral blood DNA sample was obtain followed by genotype analysis for IL4 -590 C<T using PCR RFLP, respectively IL4 70 bp VNTR determined by PCR. Results: The absolute frequency of genotypes for IL4 -590 C<T was T/T-5, C/T-42, C/C-81 in the cases group respectively T/T-2, C/T-32, C/C-77 in the control group. The chi-square test had a value of 0.983 (p=0.321) while considering the presence of a minimum one single variant allele as a risk factor for cervical cancer, respectively 0.926 (p=0.336) for homozygous variant genotype. Odds ratio was 0.761 (95%CI [0.443-1.306]) while considering C/T+T/T respectively 2R/3R, 2R/2R as a risk factor, and 0.451 (95%CI 95% [0.086-2.374]) - TT respectively 2R/2R as a risk factor. Conclusion: No linear statistical significant association has been found between IL4 polymorphism and cervical neoplasia (p = 0.322).
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
container_issue	3
title_short	The Analysis of Genetic Polymorphism. The Relationship between Interleukin – 4 Polymorphisms and Intraepithelial Cervical Neoplasia
url	https://doaj.org/article/1bfb35e137d3452298e3a8af40399f76 http://ami.info.umfcluj.ro/Full-text/AMI_27_2010/AMI(27)_043_052.pdf https://doaj.org/toc/1224-5593
remote_bool	true
author2	Gabriela CARACOSTEA Cristina BUTUZA Radu POPP Daniel MUREŞAN Sorana D. BOLBOACĂ Ioana ROTAR Felicia PETRIŞOR Marius FĂRCAŞ
author2Str	Gabriela CARACOSTEA Cristina BUTUZA Radu POPP Daniel MUREŞAN Sorana D. BOLBOACĂ Ioana ROTAR Felicia PETRIŞOR Marius FĂRCAŞ
ppnlink	629833281
callnumber-subject	R - General Medicine
mediatype_str_mv	c
isOA_txt	true
hochschulschrift_bool	false
callnumber-a	R858-859.7
up_date	2024-07-03T23:41:09.974Z
_version_	1803603222175154176
fullrecord_marcxml	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000naa a22002652 4500</leader><controlfield tag="001">DOAJ079490379</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230307014623.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230307s2010 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ079490379</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ1bfb35e137d3452298e3a8af40399f76</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">R858-859.7</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Florin STAMATIAN</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="4"><subfield code="a">The Analysis of Genetic Polymorphism. The Relationship between Interleukin – 4 Polymorphisms and Intraepithelial Cervical Neoplasia</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2010</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Objectives: Interleukin 4 plays a critical role in T helper 2 responses to HPV infection and angiogenesis. The present study aim to study the association between the IL4 promoter polymorphism – 590 C<T, respectively VNTR intron 2 polymorphism and cervical intraepithelial neoplasia. Material and method: We have realized a prospective case controls study that included 128 cases of intraepithelial neoplasia positive for HPV HR testing and 111 controls negative for intraepithelial lesion and also negative for HPV HR. Clinical examination was performed on each patient; blood and cervical sample were obtained. Cervical probes were analyzed regarding cytology and HPV HR testing. From peripheral blood DNA sample was obtain followed by genotype analysis for IL4 -590 C<T using PCR RFLP, respectively IL4 70 bp VNTR determined by PCR. Results: The absolute frequency of genotypes for IL4 -590 C<T was T/T-5, C/T-42, C/C-81 in the cases group respectively T/T-2, C/T-32, C/C-77 in the control group. The chi-square test had a value of 0.983 (p=0.321) while considering the presence of a minimum one single variant allele as a risk factor for cervical cancer, respectively 0.926 (p=0.336) for homozygous variant genotype. Odds ratio was 0.761 (95%CI [0.443-1.306]) while considering C/T+T/T respectively 2R/3R, 2R/2R as a risk factor, and 0.451 (95%CI 95% [0.086-2.374]) - TT respectively 2R/2R as a risk factor. Conclusion: No linear statistical significant association has been found between IL4 polymorphism and cervical neoplasia (p = 0.322).</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Genetic polymorphism</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Restriction fragment length polymorphism (RFLP)</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Polymerase chain reaction (PCR)</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Interleukin-4 (IL4)</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cervical intraepithelial neoplasia (CIN)</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Human papiloma virus (HPV) infection</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Human papiloma virus high risk (HPV-HR)</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Negative for intraepithelial lesion (NILM)</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Computer applications to medicine. Medical informatics</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Gabriela CARACOSTEA</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Cristina BUTUZA</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Radu POPP</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Daniel MUREŞAN</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Sorana D. BOLBOACĂ</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Ioana ROTAR</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Felicia PETRIŞOR</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Marius FĂRCAŞ</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Applied Medical Informatics</subfield><subfield code="d">Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, 2009</subfield><subfield code="g">27(2010), 3, Seite 43-52</subfield><subfield code="w">(DE-627)629833281</subfield><subfield code="w">(DE-600)2558685-3</subfield><subfield code="x">20677855</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:27</subfield><subfield code="g">year:2010</subfield><subfield code="g">number:3</subfield><subfield code="g">pages:43-52</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/1bfb35e137d3452298e3a8af40399f76</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://ami.info.umfcluj.ro/Full-text/AMI_27_2010/AMI(27)_043_052.pdf</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/1224-5593</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_206</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2005</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2009</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2011</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2055</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2111</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">27</subfield><subfield code="j">2010</subfield><subfield code="e">3</subfield><subfield code="h">43-52</subfield></datafield></record></collection>
score	7.400754

Nicht das Richtige dabei?

Schreiben Sie uns!

The Analysis of Genetic Polymorphism. The Relationship between Interleukin – 4 Polymorphisms and Intraepithelial Cervical Neoplasia

Nicht das Richtige dabei?

Zugang & Verfügbarkeit

Vorhandene Bände

Nicht das Richtige dabei?