ACE2-Inhibitory Effects of Bromelain and Ficin in Colon Cancer Cells

<i<Background and Objectives</i<: Bromelain and ficin are aqueous extracts from fruits of <i<Ananas comosus</i< and <i<Ficus carcia</i< plants, used widely for medical applications. Angiotensin-converting enzyme 2 (ACE2) is a homolog of ACE, degrading Ang II to an...
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Gespeichert in:

Autor*in:	Babak Pakbin [verfasserIn] Shaghayegh Pishkhan Dibazar [verfasserIn] Samaneh Allahyari [verfasserIn] Hanifeh Shariatifar [verfasserIn] Wolfram Manuel Brück [verfasserIn] Alireza Farasat [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	bromelain ficin ACE2-inhibitory caco-2 cell line

Übergeordnetes Werk:	In: Medicina - MDPI AG, 2016, 59(2023), 2, p 301
Übergeordnetes Werk:	volume:59 ; year:2023 ; number:2, p 301

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/medicina59020301

Katalog-ID:	DOAJ080032370

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520			\|a <i<Background and Objectives</i<: Bromelain and ficin are aqueous extracts from fruits of <i<Ananas comosus</i< and <i<Ficus carcia</i< plants, used widely for medical applications. Angiotensin-converting enzyme 2 (ACE2) is a homolog of ACE, degrading Ang II to angiotensin 1-7 and decreasing the cellular concentration of Ang II. <i<Materials and Methods</i<: In this study, we investigated the ACE2-inhibitory, antiproliferative, and apoptosis-inducing effects of ficin and bromelain on caco-2 cells. <i<Results</i<: We found that bromelain and ficin significantly reduced the viability of human colon cancer cells with IC<sub<50</sub< value concentrations of 8.8 and 4.2 mg/mL for bromelain after 24 and 48 h treatments, and 8.8 and 4.2 mg/mL for ficin after 24 and 48 h treatments, respectively. The apoptosis of the caco-2 cell line treated with bromelain was 81.04% and 56.70%, observed after 24 and 48 h. Total apoptotic proportions in caco-2 cells treated with ficin after 24 and 48 h were 83.7% and 73.0%. An amount of 1.6 mg/mL of bromelain and ficin treatments on caco-2 cells after 24 h revealed a higher decrease than that of other concentrations in the expression of ACE2 protein. <i<Conclusions</i<: In conclusion, bromelain and ficin can dose-dependently decrease the expression of ACE2 protein in caco-2 cells.
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allfields	10.3390/medicina59020301 doi (DE-627)DOAJ080032370 (DE-599)DOAJ5457b16176fc4b90be02d4059aa9fe9c DE-627 ger DE-627 rakwb eng R5-920 Babak Pakbin verfasserin aut ACE2-Inhibitory Effects of Bromelain and Ficin in Colon Cancer Cells 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Background and Objectives</i<: Bromelain and ficin are aqueous extracts from fruits of <i<Ananas comosus</i< and <i<Ficus carcia</i< plants, used widely for medical applications. Angiotensin-converting enzyme 2 (ACE2) is a homolog of ACE, degrading Ang II to angiotensin 1-7 and decreasing the cellular concentration of Ang II. <i<Materials and Methods</i<: In this study, we investigated the ACE2-inhibitory, antiproliferative, and apoptosis-inducing effects of ficin and bromelain on caco-2 cells. <i<Results</i<: We found that bromelain and ficin significantly reduced the viability of human colon cancer cells with IC<sub<50</sub< value concentrations of 8.8 and 4.2 mg/mL for bromelain after 24 and 48 h treatments, and 8.8 and 4.2 mg/mL for ficin after 24 and 48 h treatments, respectively. The apoptosis of the caco-2 cell line treated with bromelain was 81.04% and 56.70%, observed after 24 and 48 h. Total apoptotic proportions in caco-2 cells treated with ficin after 24 and 48 h were 83.7% and 73.0%. An amount of 1.6 mg/mL of bromelain and ficin treatments on caco-2 cells after 24 h revealed a higher decrease than that of other concentrations in the expression of ACE2 protein. <i<Conclusions</i<: In conclusion, bromelain and ficin can dose-dependently decrease the expression of ACE2 protein in caco-2 cells. bromelain ficin ACE2-inhibitory caco-2 cell line Medicine (General) Shaghayegh Pishkhan Dibazar verfasserin aut Samaneh Allahyari verfasserin aut Hanifeh Shariatifar verfasserin aut Wolfram Manuel Brück verfasserin aut Alireza Farasat verfasserin aut In Medicina MDPI AG, 2016 59(2023), 2, p 301 (DE-627)354543296 (DE-600)2088820-X 16489144 nnns volume:59 year:2023 number:2, p 301 https://doi.org/10.3390/medicina59020301 kostenfrei https://doaj.org/article/5457b16176fc4b90be02d4059aa9fe9c kostenfrei https://www.mdpi.com/1648-9144/59/2/301 kostenfrei https://doaj.org/toc/1010-660X Journal toc kostenfrei https://doaj.org/toc/1648-9144 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 59 2023 2, p 301
spelling	10.3390/medicina59020301 doi (DE-627)DOAJ080032370 (DE-599)DOAJ5457b16176fc4b90be02d4059aa9fe9c DE-627 ger DE-627 rakwb eng R5-920 Babak Pakbin verfasserin aut ACE2-Inhibitory Effects of Bromelain and Ficin in Colon Cancer Cells 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Background and Objectives</i<: Bromelain and ficin are aqueous extracts from fruits of <i<Ananas comosus</i< and <i<Ficus carcia</i< plants, used widely for medical applications. Angiotensin-converting enzyme 2 (ACE2) is a homolog of ACE, degrading Ang II to angiotensin 1-7 and decreasing the cellular concentration of Ang II. <i<Materials and Methods</i<: In this study, we investigated the ACE2-inhibitory, antiproliferative, and apoptosis-inducing effects of ficin and bromelain on caco-2 cells. <i<Results</i<: We found that bromelain and ficin significantly reduced the viability of human colon cancer cells with IC<sub<50</sub< value concentrations of 8.8 and 4.2 mg/mL for bromelain after 24 and 48 h treatments, and 8.8 and 4.2 mg/mL for ficin after 24 and 48 h treatments, respectively. The apoptosis of the caco-2 cell line treated with bromelain was 81.04% and 56.70%, observed after 24 and 48 h. Total apoptotic proportions in caco-2 cells treated with ficin after 24 and 48 h were 83.7% and 73.0%. An amount of 1.6 mg/mL of bromelain and ficin treatments on caco-2 cells after 24 h revealed a higher decrease than that of other concentrations in the expression of ACE2 protein. <i<Conclusions</i<: In conclusion, bromelain and ficin can dose-dependently decrease the expression of ACE2 protein in caco-2 cells. bromelain ficin ACE2-inhibitory caco-2 cell line Medicine (General) Shaghayegh Pishkhan Dibazar verfasserin aut Samaneh Allahyari verfasserin aut Hanifeh Shariatifar verfasserin aut Wolfram Manuel Brück verfasserin aut Alireza Farasat verfasserin aut In Medicina MDPI AG, 2016 59(2023), 2, p 301 (DE-627)354543296 (DE-600)2088820-X 16489144 nnns volume:59 year:2023 number:2, p 301 https://doi.org/10.3390/medicina59020301 kostenfrei https://doaj.org/article/5457b16176fc4b90be02d4059aa9fe9c kostenfrei https://www.mdpi.com/1648-9144/59/2/301 kostenfrei https://doaj.org/toc/1010-660X Journal toc kostenfrei https://doaj.org/toc/1648-9144 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 59 2023 2, p 301
allfields_unstemmed	10.3390/medicina59020301 doi (DE-627)DOAJ080032370 (DE-599)DOAJ5457b16176fc4b90be02d4059aa9fe9c DE-627 ger DE-627 rakwb eng R5-920 Babak Pakbin verfasserin aut ACE2-Inhibitory Effects of Bromelain and Ficin in Colon Cancer Cells 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Background and Objectives</i<: Bromelain and ficin are aqueous extracts from fruits of <i<Ananas comosus</i< and <i<Ficus carcia</i< plants, used widely for medical applications. Angiotensin-converting enzyme 2 (ACE2) is a homolog of ACE, degrading Ang II to angiotensin 1-7 and decreasing the cellular concentration of Ang II. <i<Materials and Methods</i<: In this study, we investigated the ACE2-inhibitory, antiproliferative, and apoptosis-inducing effects of ficin and bromelain on caco-2 cells. <i<Results</i<: We found that bromelain and ficin significantly reduced the viability of human colon cancer cells with IC<sub<50</sub< value concentrations of 8.8 and 4.2 mg/mL for bromelain after 24 and 48 h treatments, and 8.8 and 4.2 mg/mL for ficin after 24 and 48 h treatments, respectively. The apoptosis of the caco-2 cell line treated with bromelain was 81.04% and 56.70%, observed after 24 and 48 h. Total apoptotic proportions in caco-2 cells treated with ficin after 24 and 48 h were 83.7% and 73.0%. An amount of 1.6 mg/mL of bromelain and ficin treatments on caco-2 cells after 24 h revealed a higher decrease than that of other concentrations in the expression of ACE2 protein. <i<Conclusions</i<: In conclusion, bromelain and ficin can dose-dependently decrease the expression of ACE2 protein in caco-2 cells. bromelain ficin ACE2-inhibitory caco-2 cell line Medicine (General) Shaghayegh Pishkhan Dibazar verfasserin aut Samaneh Allahyari verfasserin aut Hanifeh Shariatifar verfasserin aut Wolfram Manuel Brück verfasserin aut Alireza Farasat verfasserin aut In Medicina MDPI AG, 2016 59(2023), 2, p 301 (DE-627)354543296 (DE-600)2088820-X 16489144 nnns volume:59 year:2023 number:2, p 301 https://doi.org/10.3390/medicina59020301 kostenfrei https://doaj.org/article/5457b16176fc4b90be02d4059aa9fe9c kostenfrei https://www.mdpi.com/1648-9144/59/2/301 kostenfrei https://doaj.org/toc/1010-660X Journal toc kostenfrei https://doaj.org/toc/1648-9144 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 59 2023 2, p 301
allfieldsGer	10.3390/medicina59020301 doi (DE-627)DOAJ080032370 (DE-599)DOAJ5457b16176fc4b90be02d4059aa9fe9c DE-627 ger DE-627 rakwb eng R5-920 Babak Pakbin verfasserin aut ACE2-Inhibitory Effects of Bromelain and Ficin in Colon Cancer Cells 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Background and Objectives</i<: Bromelain and ficin are aqueous extracts from fruits of <i<Ananas comosus</i< and <i<Ficus carcia</i< plants, used widely for medical applications. Angiotensin-converting enzyme 2 (ACE2) is a homolog of ACE, degrading Ang II to angiotensin 1-7 and decreasing the cellular concentration of Ang II. <i<Materials and Methods</i<: In this study, we investigated the ACE2-inhibitory, antiproliferative, and apoptosis-inducing effects of ficin and bromelain on caco-2 cells. <i<Results</i<: We found that bromelain and ficin significantly reduced the viability of human colon cancer cells with IC<sub<50</sub< value concentrations of 8.8 and 4.2 mg/mL for bromelain after 24 and 48 h treatments, and 8.8 and 4.2 mg/mL for ficin after 24 and 48 h treatments, respectively. The apoptosis of the caco-2 cell line treated with bromelain was 81.04% and 56.70%, observed after 24 and 48 h. Total apoptotic proportions in caco-2 cells treated with ficin after 24 and 48 h were 83.7% and 73.0%. An amount of 1.6 mg/mL of bromelain and ficin treatments on caco-2 cells after 24 h revealed a higher decrease than that of other concentrations in the expression of ACE2 protein. <i<Conclusions</i<: In conclusion, bromelain and ficin can dose-dependently decrease the expression of ACE2 protein in caco-2 cells. bromelain ficin ACE2-inhibitory caco-2 cell line Medicine (General) Shaghayegh Pishkhan Dibazar verfasserin aut Samaneh Allahyari verfasserin aut Hanifeh Shariatifar verfasserin aut Wolfram Manuel Brück verfasserin aut Alireza Farasat verfasserin aut In Medicina MDPI AG, 2016 59(2023), 2, p 301 (DE-627)354543296 (DE-600)2088820-X 16489144 nnns volume:59 year:2023 number:2, p 301 https://doi.org/10.3390/medicina59020301 kostenfrei https://doaj.org/article/5457b16176fc4b90be02d4059aa9fe9c kostenfrei https://www.mdpi.com/1648-9144/59/2/301 kostenfrei https://doaj.org/toc/1010-660X Journal toc kostenfrei https://doaj.org/toc/1648-9144 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 59 2023 2, p 301
allfieldsSound	10.3390/medicina59020301 doi (DE-627)DOAJ080032370 (DE-599)DOAJ5457b16176fc4b90be02d4059aa9fe9c DE-627 ger DE-627 rakwb eng R5-920 Babak Pakbin verfasserin aut ACE2-Inhibitory Effects of Bromelain and Ficin in Colon Cancer Cells 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Background and Objectives</i<: Bromelain and ficin are aqueous extracts from fruits of <i<Ananas comosus</i< and <i<Ficus carcia</i< plants, used widely for medical applications. Angiotensin-converting enzyme 2 (ACE2) is a homolog of ACE, degrading Ang II to angiotensin 1-7 and decreasing the cellular concentration of Ang II. <i<Materials and Methods</i<: In this study, we investigated the ACE2-inhibitory, antiproliferative, and apoptosis-inducing effects of ficin and bromelain on caco-2 cells. <i<Results</i<: We found that bromelain and ficin significantly reduced the viability of human colon cancer cells with IC<sub<50</sub< value concentrations of 8.8 and 4.2 mg/mL for bromelain after 24 and 48 h treatments, and 8.8 and 4.2 mg/mL for ficin after 24 and 48 h treatments, respectively. The apoptosis of the caco-2 cell line treated with bromelain was 81.04% and 56.70%, observed after 24 and 48 h. Total apoptotic proportions in caco-2 cells treated with ficin after 24 and 48 h were 83.7% and 73.0%. An amount of 1.6 mg/mL of bromelain and ficin treatments on caco-2 cells after 24 h revealed a higher decrease than that of other concentrations in the expression of ACE2 protein. <i<Conclusions</i<: In conclusion, bromelain and ficin can dose-dependently decrease the expression of ACE2 protein in caco-2 cells. bromelain ficin ACE2-inhibitory caco-2 cell line Medicine (General) Shaghayegh Pishkhan Dibazar verfasserin aut Samaneh Allahyari verfasserin aut Hanifeh Shariatifar verfasserin aut Wolfram Manuel Brück verfasserin aut Alireza Farasat verfasserin aut In Medicina MDPI AG, 2016 59(2023), 2, p 301 (DE-627)354543296 (DE-600)2088820-X 16489144 nnns volume:59 year:2023 number:2, p 301 https://doi.org/10.3390/medicina59020301 kostenfrei https://doaj.org/article/5457b16176fc4b90be02d4059aa9fe9c kostenfrei https://www.mdpi.com/1648-9144/59/2/301 kostenfrei https://doaj.org/toc/1010-660X Journal toc kostenfrei https://doaj.org/toc/1648-9144 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 59 2023 2, p 301
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callnumber-first	R - Medicine
author	Babak Pakbin
spellingShingle	Babak Pakbin misc R5-920 misc bromelain misc ficin misc ACE2-inhibitory misc caco-2 cell line misc Medicine (General) ACE2-Inhibitory Effects of Bromelain and Ficin in Colon Cancer Cells
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topic_title	R5-920 ACE2-Inhibitory Effects of Bromelain and Ficin in Colon Cancer Cells bromelain ficin ACE2-inhibitory caco-2 cell line
topic	misc R5-920 misc bromelain misc ficin misc ACE2-inhibitory misc caco-2 cell line misc Medicine (General)
topic_unstemmed	misc R5-920 misc bromelain misc ficin misc ACE2-inhibitory misc caco-2 cell line misc Medicine (General)
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title	ACE2-Inhibitory Effects of Bromelain and Ficin in Colon Cancer Cells
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title_full	ACE2-Inhibitory Effects of Bromelain and Ficin in Colon Cancer Cells
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author_browse	Babak Pakbin Shaghayegh Pishkhan Dibazar Samaneh Allahyari Hanifeh Shariatifar Wolfram Manuel Brück Alireza Farasat
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title_sort	ace2-inhibitory effects of bromelain and ficin in colon cancer cells
callnumber	R5-920
title_auth	ACE2-Inhibitory Effects of Bromelain and Ficin in Colon Cancer Cells
abstract	<i<Background and Objectives</i<: Bromelain and ficin are aqueous extracts from fruits of <i<Ananas comosus</i< and <i<Ficus carcia</i< plants, used widely for medical applications. Angiotensin-converting enzyme 2 (ACE2) is a homolog of ACE, degrading Ang II to angiotensin 1-7 and decreasing the cellular concentration of Ang II. <i<Materials and Methods</i<: In this study, we investigated the ACE2-inhibitory, antiproliferative, and apoptosis-inducing effects of ficin and bromelain on caco-2 cells. <i<Results</i<: We found that bromelain and ficin significantly reduced the viability of human colon cancer cells with IC<sub<50</sub< value concentrations of 8.8 and 4.2 mg/mL for bromelain after 24 and 48 h treatments, and 8.8 and 4.2 mg/mL for ficin after 24 and 48 h treatments, respectively. The apoptosis of the caco-2 cell line treated with bromelain was 81.04% and 56.70%, observed after 24 and 48 h. Total apoptotic proportions in caco-2 cells treated with ficin after 24 and 48 h were 83.7% and 73.0%. An amount of 1.6 mg/mL of bromelain and ficin treatments on caco-2 cells after 24 h revealed a higher decrease than that of other concentrations in the expression of ACE2 protein. <i<Conclusions</i<: In conclusion, bromelain and ficin can dose-dependently decrease the expression of ACE2 protein in caco-2 cells.
abstractGer	<i<Background and Objectives</i<: Bromelain and ficin are aqueous extracts from fruits of <i<Ananas comosus</i< and <i<Ficus carcia</i< plants, used widely for medical applications. Angiotensin-converting enzyme 2 (ACE2) is a homolog of ACE, degrading Ang II to angiotensin 1-7 and decreasing the cellular concentration of Ang II. <i<Materials and Methods</i<: In this study, we investigated the ACE2-inhibitory, antiproliferative, and apoptosis-inducing effects of ficin and bromelain on caco-2 cells. <i<Results</i<: We found that bromelain and ficin significantly reduced the viability of human colon cancer cells with IC<sub<50</sub< value concentrations of 8.8 and 4.2 mg/mL for bromelain after 24 and 48 h treatments, and 8.8 and 4.2 mg/mL for ficin after 24 and 48 h treatments, respectively. The apoptosis of the caco-2 cell line treated with bromelain was 81.04% and 56.70%, observed after 24 and 48 h. Total apoptotic proportions in caco-2 cells treated with ficin after 24 and 48 h were 83.7% and 73.0%. An amount of 1.6 mg/mL of bromelain and ficin treatments on caco-2 cells after 24 h revealed a higher decrease than that of other concentrations in the expression of ACE2 protein. <i<Conclusions</i<: In conclusion, bromelain and ficin can dose-dependently decrease the expression of ACE2 protein in caco-2 cells.
abstract_unstemmed	<i<Background and Objectives</i<: Bromelain and ficin are aqueous extracts from fruits of <i<Ananas comosus</i< and <i<Ficus carcia</i< plants, used widely for medical applications. Angiotensin-converting enzyme 2 (ACE2) is a homolog of ACE, degrading Ang II to angiotensin 1-7 and decreasing the cellular concentration of Ang II. <i<Materials and Methods</i<: In this study, we investigated the ACE2-inhibitory, antiproliferative, and apoptosis-inducing effects of ficin and bromelain on caco-2 cells. <i<Results</i<: We found that bromelain and ficin significantly reduced the viability of human colon cancer cells with IC<sub<50</sub< value concentrations of 8.8 and 4.2 mg/mL for bromelain after 24 and 48 h treatments, and 8.8 and 4.2 mg/mL for ficin after 24 and 48 h treatments, respectively. The apoptosis of the caco-2 cell line treated with bromelain was 81.04% and 56.70%, observed after 24 and 48 h. Total apoptotic proportions in caco-2 cells treated with ficin after 24 and 48 h were 83.7% and 73.0%. An amount of 1.6 mg/mL of bromelain and ficin treatments on caco-2 cells after 24 h revealed a higher decrease than that of other concentrations in the expression of ACE2 protein. <i<Conclusions</i<: In conclusion, bromelain and ficin can dose-dependently decrease the expression of ACE2 protein in caco-2 cells.
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title_short	ACE2-Inhibitory Effects of Bromelain and Ficin in Colon Cancer Cells
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author2	Shaghayegh Pishkhan Dibazar Samaneh Allahyari Hanifeh Shariatifar Wolfram Manuel Brück Alireza Farasat
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ACE2-Inhibitory Effects of Bromelain and Ficin in Colon Cancer Cells

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