Long-term culture-initiating cells (LTC-IC) produced from CD34+ cord blood cells with limiting dilution method
OBJECTIVE: Even though much progress has been made in defining primitive hematologic cell phenotypes by using flow cytometry and clonogenic methods, the direct method for study of marrow repopulating cells still remains to be elusive. Long Term Culture-Initiating Cells (LTC-IC) are known as the most...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Gülderen Yanıkkaya Demirel [verfasserIn] Tülin Budak-Alpdoğan [verfasserIn] Sema Aktaş [verfasserIn] Mahmut Bayık [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2010 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
In: Turkish Journal of Hematology ; 27(2010), 4, Seite 234-241 volume:27 ; year:2010 ; number:4 ; pages:234-241 |
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| DOI / URN: |
10.5152/tjh.2010.44 |
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DOAJ080180477 |
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| 520 | |a OBJECTIVE: Even though much progress has been made in defining primitive hematologic cell phenotypes by using flow cytometry and clonogenic methods, the direct method for study of marrow repopulating cells still remains to be elusive. Long Term Culture-Initiating Cells (LTC-IC) are known as the most primitive human hematopoietic cells detectable by in vitro functional assays. METHODS: In this study, LTC-IC with limiting dilution assay was used to evaluate repopulating potential of cord blood stem cells. RESULTS: CD34 selections from cord blood were completed succesfully with magnetic beads (73,64%+-9,12). The average incidence of week 5 LTC-IC was 1: 1966 CD34+ cells (range 1261–2906). CONCLUSION: We found that number of LTC-IC obtained from CD34+ cord blood cells were relatively low in numbers when compared to previously reported bone marrow CD34+ cells. This may be due to the lack of some transcription and growth factors along with some cytokines and chemokines released by accessory cells which are necessary for proliferation of cord blood progenitor/stem cells and it presents an area of interest for further studies. | ||
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10.5152/tjh.2010.44 doi (DE-627)DOAJ080180477 (DE-599)DOAJa358be6ae833422b96d5f1988cd8e4fd DE-627 ger DE-627 rakwb eng RC633-647.5 Gülderen Yanıkkaya Demirel verfasserin aut Long-term culture-initiating cells (LTC-IC) produced from CD34+ cord blood cells with limiting dilution method 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier OBJECTIVE: Even though much progress has been made in defining primitive hematologic cell phenotypes by using flow cytometry and clonogenic methods, the direct method for study of marrow repopulating cells still remains to be elusive. Long Term Culture-Initiating Cells (LTC-IC) are known as the most primitive human hematopoietic cells detectable by in vitro functional assays. METHODS: In this study, LTC-IC with limiting dilution assay was used to evaluate repopulating potential of cord blood stem cells. RESULTS: CD34 selections from cord blood were completed succesfully with magnetic beads (73,64%+-9,12). The average incidence of week 5 LTC-IC was 1: 1966 CD34+ cells (range 1261–2906). CONCLUSION: We found that number of LTC-IC obtained from CD34+ cord blood cells were relatively low in numbers when compared to previously reported bone marrow CD34+ cells. This may be due to the lack of some transcription and growth factors along with some cytokines and chemokines released by accessory cells which are necessary for proliferation of cord blood progenitor/stem cells and it presents an area of interest for further studies. stem cell cord blood ltc-ic limiting dilution cfu assay cytometry Diseases of the blood and blood-forming organs Tülin Budak-Alpdoğan verfasserin aut Sema Aktaş verfasserin aut Mahmut Bayık verfasserin aut In Turkish Journal of Hematology 27(2010), 4, Seite 234-241 volume:27 year:2010 number:4 pages:234-241 https://doi.org/10.5152/tjh.2010.44 kostenfrei https://doaj.org/article/a358be6ae833422b96d5f1988cd8e4fd kostenfrei https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-35002 kostenfrei https://doaj.org/toc/1308-5263 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 27 2010 4 234-241 |
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10.5152/tjh.2010.44 doi (DE-627)DOAJ080180477 (DE-599)DOAJa358be6ae833422b96d5f1988cd8e4fd DE-627 ger DE-627 rakwb eng RC633-647.5 Gülderen Yanıkkaya Demirel verfasserin aut Long-term culture-initiating cells (LTC-IC) produced from CD34+ cord blood cells with limiting dilution method 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier OBJECTIVE: Even though much progress has been made in defining primitive hematologic cell phenotypes by using flow cytometry and clonogenic methods, the direct method for study of marrow repopulating cells still remains to be elusive. Long Term Culture-Initiating Cells (LTC-IC) are known as the most primitive human hematopoietic cells detectable by in vitro functional assays. METHODS: In this study, LTC-IC with limiting dilution assay was used to evaluate repopulating potential of cord blood stem cells. RESULTS: CD34 selections from cord blood were completed succesfully with magnetic beads (73,64%+-9,12). The average incidence of week 5 LTC-IC was 1: 1966 CD34+ cells (range 1261–2906). CONCLUSION: We found that number of LTC-IC obtained from CD34+ cord blood cells were relatively low in numbers when compared to previously reported bone marrow CD34+ cells. This may be due to the lack of some transcription and growth factors along with some cytokines and chemokines released by accessory cells which are necessary for proliferation of cord blood progenitor/stem cells and it presents an area of interest for further studies. stem cell cord blood ltc-ic limiting dilution cfu assay cytometry Diseases of the blood and blood-forming organs Tülin Budak-Alpdoğan verfasserin aut Sema Aktaş verfasserin aut Mahmut Bayık verfasserin aut In Turkish Journal of Hematology 27(2010), 4, Seite 234-241 volume:27 year:2010 number:4 pages:234-241 https://doi.org/10.5152/tjh.2010.44 kostenfrei https://doaj.org/article/a358be6ae833422b96d5f1988cd8e4fd kostenfrei https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-35002 kostenfrei https://doaj.org/toc/1308-5263 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 27 2010 4 234-241 |
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10.5152/tjh.2010.44 doi (DE-627)DOAJ080180477 (DE-599)DOAJa358be6ae833422b96d5f1988cd8e4fd DE-627 ger DE-627 rakwb eng RC633-647.5 Gülderen Yanıkkaya Demirel verfasserin aut Long-term culture-initiating cells (LTC-IC) produced from CD34+ cord blood cells with limiting dilution method 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier OBJECTIVE: Even though much progress has been made in defining primitive hematologic cell phenotypes by using flow cytometry and clonogenic methods, the direct method for study of marrow repopulating cells still remains to be elusive. Long Term Culture-Initiating Cells (LTC-IC) are known as the most primitive human hematopoietic cells detectable by in vitro functional assays. METHODS: In this study, LTC-IC with limiting dilution assay was used to evaluate repopulating potential of cord blood stem cells. RESULTS: CD34 selections from cord blood were completed succesfully with magnetic beads (73,64%+-9,12). The average incidence of week 5 LTC-IC was 1: 1966 CD34+ cells (range 1261–2906). CONCLUSION: We found that number of LTC-IC obtained from CD34+ cord blood cells were relatively low in numbers when compared to previously reported bone marrow CD34+ cells. This may be due to the lack of some transcription and growth factors along with some cytokines and chemokines released by accessory cells which are necessary for proliferation of cord blood progenitor/stem cells and it presents an area of interest for further studies. stem cell cord blood ltc-ic limiting dilution cfu assay cytometry Diseases of the blood and blood-forming organs Tülin Budak-Alpdoğan verfasserin aut Sema Aktaş verfasserin aut Mahmut Bayık verfasserin aut In Turkish Journal of Hematology 27(2010), 4, Seite 234-241 volume:27 year:2010 number:4 pages:234-241 https://doi.org/10.5152/tjh.2010.44 kostenfrei https://doaj.org/article/a358be6ae833422b96d5f1988cd8e4fd kostenfrei https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-35002 kostenfrei https://doaj.org/toc/1308-5263 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 27 2010 4 234-241 |
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10.5152/tjh.2010.44 doi (DE-627)DOAJ080180477 (DE-599)DOAJa358be6ae833422b96d5f1988cd8e4fd DE-627 ger DE-627 rakwb eng RC633-647.5 Gülderen Yanıkkaya Demirel verfasserin aut Long-term culture-initiating cells (LTC-IC) produced from CD34+ cord blood cells with limiting dilution method 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier OBJECTIVE: Even though much progress has been made in defining primitive hematologic cell phenotypes by using flow cytometry and clonogenic methods, the direct method for study of marrow repopulating cells still remains to be elusive. Long Term Culture-Initiating Cells (LTC-IC) are known as the most primitive human hematopoietic cells detectable by in vitro functional assays. METHODS: In this study, LTC-IC with limiting dilution assay was used to evaluate repopulating potential of cord blood stem cells. RESULTS: CD34 selections from cord blood were completed succesfully with magnetic beads (73,64%+-9,12). The average incidence of week 5 LTC-IC was 1: 1966 CD34+ cells (range 1261–2906). CONCLUSION: We found that number of LTC-IC obtained from CD34+ cord blood cells were relatively low in numbers when compared to previously reported bone marrow CD34+ cells. This may be due to the lack of some transcription and growth factors along with some cytokines and chemokines released by accessory cells which are necessary for proliferation of cord blood progenitor/stem cells and it presents an area of interest for further studies. stem cell cord blood ltc-ic limiting dilution cfu assay cytometry Diseases of the blood and blood-forming organs Tülin Budak-Alpdoğan verfasserin aut Sema Aktaş verfasserin aut Mahmut Bayık verfasserin aut In Turkish Journal of Hematology 27(2010), 4, Seite 234-241 volume:27 year:2010 number:4 pages:234-241 https://doi.org/10.5152/tjh.2010.44 kostenfrei https://doaj.org/article/a358be6ae833422b96d5f1988cd8e4fd kostenfrei https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-35002 kostenfrei https://doaj.org/toc/1308-5263 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 27 2010 4 234-241 |
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10.5152/tjh.2010.44 doi (DE-627)DOAJ080180477 (DE-599)DOAJa358be6ae833422b96d5f1988cd8e4fd DE-627 ger DE-627 rakwb eng RC633-647.5 Gülderen Yanıkkaya Demirel verfasserin aut Long-term culture-initiating cells (LTC-IC) produced from CD34+ cord blood cells with limiting dilution method 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier OBJECTIVE: Even though much progress has been made in defining primitive hematologic cell phenotypes by using flow cytometry and clonogenic methods, the direct method for study of marrow repopulating cells still remains to be elusive. Long Term Culture-Initiating Cells (LTC-IC) are known as the most primitive human hematopoietic cells detectable by in vitro functional assays. METHODS: In this study, LTC-IC with limiting dilution assay was used to evaluate repopulating potential of cord blood stem cells. RESULTS: CD34 selections from cord blood were completed succesfully with magnetic beads (73,64%+-9,12). The average incidence of week 5 LTC-IC was 1: 1966 CD34+ cells (range 1261–2906). CONCLUSION: We found that number of LTC-IC obtained from CD34+ cord blood cells were relatively low in numbers when compared to previously reported bone marrow CD34+ cells. This may be due to the lack of some transcription and growth factors along with some cytokines and chemokines released by accessory cells which are necessary for proliferation of cord blood progenitor/stem cells and it presents an area of interest for further studies. stem cell cord blood ltc-ic limiting dilution cfu assay cytometry Diseases of the blood and blood-forming organs Tülin Budak-Alpdoğan verfasserin aut Sema Aktaş verfasserin aut Mahmut Bayık verfasserin aut In Turkish Journal of Hematology 27(2010), 4, Seite 234-241 volume:27 year:2010 number:4 pages:234-241 https://doi.org/10.5152/tjh.2010.44 kostenfrei https://doaj.org/article/a358be6ae833422b96d5f1988cd8e4fd kostenfrei https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-35002 kostenfrei https://doaj.org/toc/1308-5263 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 27 2010 4 234-241 |
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Long-term culture-initiating cells (LTC-IC) produced from CD34+ cord blood cells with limiting dilution method |
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OBJECTIVE: Even though much progress has been made in defining primitive hematologic cell phenotypes by using flow cytometry and clonogenic methods, the direct method for study of marrow repopulating cells still remains to be elusive. Long Term Culture-Initiating Cells (LTC-IC) are known as the most primitive human hematopoietic cells detectable by in vitro functional assays. METHODS: In this study, LTC-IC with limiting dilution assay was used to evaluate repopulating potential of cord blood stem cells. RESULTS: CD34 selections from cord blood were completed succesfully with magnetic beads (73,64%+-9,12). The average incidence of week 5 LTC-IC was 1: 1966 CD34+ cells (range 1261–2906). CONCLUSION: We found that number of LTC-IC obtained from CD34+ cord blood cells were relatively low in numbers when compared to previously reported bone marrow CD34+ cells. This may be due to the lack of some transcription and growth factors along with some cytokines and chemokines released by accessory cells which are necessary for proliferation of cord blood progenitor/stem cells and it presents an area of interest for further studies. |
| abstractGer |
OBJECTIVE: Even though much progress has been made in defining primitive hematologic cell phenotypes by using flow cytometry and clonogenic methods, the direct method for study of marrow repopulating cells still remains to be elusive. Long Term Culture-Initiating Cells (LTC-IC) are known as the most primitive human hematopoietic cells detectable by in vitro functional assays. METHODS: In this study, LTC-IC with limiting dilution assay was used to evaluate repopulating potential of cord blood stem cells. RESULTS: CD34 selections from cord blood were completed succesfully with magnetic beads (73,64%+-9,12). The average incidence of week 5 LTC-IC was 1: 1966 CD34+ cells (range 1261–2906). CONCLUSION: We found that number of LTC-IC obtained from CD34+ cord blood cells were relatively low in numbers when compared to previously reported bone marrow CD34+ cells. This may be due to the lack of some transcription and growth factors along with some cytokines and chemokines released by accessory cells which are necessary for proliferation of cord blood progenitor/stem cells and it presents an area of interest for further studies. |
| abstract_unstemmed |
OBJECTIVE: Even though much progress has been made in defining primitive hematologic cell phenotypes by using flow cytometry and clonogenic methods, the direct method for study of marrow repopulating cells still remains to be elusive. Long Term Culture-Initiating Cells (LTC-IC) are known as the most primitive human hematopoietic cells detectable by in vitro functional assays. METHODS: In this study, LTC-IC with limiting dilution assay was used to evaluate repopulating potential of cord blood stem cells. RESULTS: CD34 selections from cord blood were completed succesfully with magnetic beads (73,64%+-9,12). The average incidence of week 5 LTC-IC was 1: 1966 CD34+ cells (range 1261–2906). CONCLUSION: We found that number of LTC-IC obtained from CD34+ cord blood cells were relatively low in numbers when compared to previously reported bone marrow CD34+ cells. This may be due to the lack of some transcription and growth factors along with some cytokines and chemokines released by accessory cells which are necessary for proliferation of cord blood progenitor/stem cells and it presents an area of interest for further studies. |
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Long-term culture-initiating cells (LTC-IC) produced from CD34+ cord blood cells with limiting dilution method |
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https://doi.org/10.5152/tjh.2010.44 https://doaj.org/article/a358be6ae833422b96d5f1988cd8e4fd https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-35002 https://doaj.org/toc/1308-5263 |
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| author2 |
Tülin Budak-Alpdoğan Sema Aktaş Mahmut Bayık |
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Tülin Budak-Alpdoğan Sema Aktaş Mahmut Bayık |
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RC - Internal Medicine |
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10.5152/tjh.2010.44 |
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RC633-647.5 |
| up_date |
2024-07-04T02:19:09.224Z |
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