A Survey of Didemnin Depsipeptide Production in <em<Tistrella</em<
As one of the first families of marine natural products to undergo clinical trials, the didemnin depsipeptides have played a significant role in inspiring the discovery of marine drugs. Originally developed as anticancer therapeutics, the recent re-evaluation of these compounds including synthetical...
Ausführliche Beschreibung
Autor*in: |
Robert J. Stankey [verfasserIn] Don Johnson [verfasserIn] Brendan M. Duggan [verfasserIn] David A. Mead [verfasserIn] James J. La Clair [verfasserIn] |
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Format: |
E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2023 |
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Übergeordnetes Werk: |
In: Marine Drugs - MDPI AG, 2005, 21(2023), 2, p 56 |
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Übergeordnetes Werk: |
volume:21 ; year:2023 ; number:2, p 56 |
Links: |
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DOI / URN: |
10.3390/md21020056 |
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Katalog-ID: |
DOAJ080226639 |
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A Survey of Didemnin Depsipeptide Production in <em<Tistrella</em< |
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As one of the first families of marine natural products to undergo clinical trials, the didemnin depsipeptides have played a significant role in inspiring the discovery of marine drugs. Originally developed as anticancer therapeutics, the recent re-evaluation of these compounds including synthetically derived dehydrodidemnin B or Aplidine, has led to their advancement towards antiviral applications. While conventionally associated with production in colonial tunicates of the family <i<Didemnidae</i<, recent studies have identified their biosynthetic gene clusters from the marine–derived bacteria <i<Tistrella mobilis</i<. While these studies confirm the production of didemnin X/Y, the low titer and general lack of understanding of their biosynthesis in <i<Tistrella</i< currently prevents the development of effective microbial or synthetic biological approaches for their production. To this end, we conducted a survey of known species of <i<Tistrella</i< and report on their ability to produce the didemnin depsipeptides. These data were used to develop conditions to produce didemnin B at titers over 15 mg/L. |
abstractGer |
As one of the first families of marine natural products to undergo clinical trials, the didemnin depsipeptides have played a significant role in inspiring the discovery of marine drugs. Originally developed as anticancer therapeutics, the recent re-evaluation of these compounds including synthetically derived dehydrodidemnin B or Aplidine, has led to their advancement towards antiviral applications. While conventionally associated with production in colonial tunicates of the family <i<Didemnidae</i<, recent studies have identified their biosynthetic gene clusters from the marine–derived bacteria <i<Tistrella mobilis</i<. While these studies confirm the production of didemnin X/Y, the low titer and general lack of understanding of their biosynthesis in <i<Tistrella</i< currently prevents the development of effective microbial or synthetic biological approaches for their production. To this end, we conducted a survey of known species of <i<Tistrella</i< and report on their ability to produce the didemnin depsipeptides. These data were used to develop conditions to produce didemnin B at titers over 15 mg/L. |
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As one of the first families of marine natural products to undergo clinical trials, the didemnin depsipeptides have played a significant role in inspiring the discovery of marine drugs. Originally developed as anticancer therapeutics, the recent re-evaluation of these compounds including synthetically derived dehydrodidemnin B or Aplidine, has led to their advancement towards antiviral applications. While conventionally associated with production in colonial tunicates of the family <i<Didemnidae</i<, recent studies have identified their biosynthetic gene clusters from the marine–derived bacteria <i<Tistrella mobilis</i<. While these studies confirm the production of didemnin X/Y, the low titer and general lack of understanding of their biosynthesis in <i<Tistrella</i< currently prevents the development of effective microbial or synthetic biological approaches for their production. To this end, we conducted a survey of known species of <i<Tistrella</i< and report on their ability to produce the didemnin depsipeptides. These data were used to develop conditions to produce didemnin B at titers over 15 mg/L. |
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7.4010954 |