NUF2 Promotes Breast Cancer Development as a New Tumor Stem Cell Indicator

Multiple new subtypes of breast cancer (BRCA) are identified in women each year, rendering BRCA the most common and rapidly expanding form of cancer in females globally. NUF2 has been identified as a prognostic factor in various human cancers, regulating cell apoptosis and proliferation. However, it...
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Autor*in:	Yang Deng [verfasserIn] Jiapeng Li [verfasserIn] Yingjie Zhang [verfasserIn] Hao Hu [verfasserIn] Fujian Wan [verfasserIn] Hang Min [verfasserIn] Hao Zhou [verfasserIn] Lixing Gu [verfasserIn] Xinghua Liao [verfasserIn] Jingjiao Zhou [verfasserIn] Jun Zhou [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	breast cancer NUF2 cancer stemness

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 24(2023), 4, p 4226
Übergeordnetes Werk:	volume:24 ; year:2023 ; number:4, p 4226

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms24044226

Katalog-ID:	DOAJ080253512

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allfields	10.3390/ijms24044226 doi (DE-627)DOAJ080253512 (DE-599)DOAJcd19cac0a5d6483db1981ad7d42d3377 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Yang Deng verfasserin aut NUF2 Promotes Breast Cancer Development as a New Tumor Stem Cell Indicator 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Multiple new subtypes of breast cancer (BRCA) are identified in women each year, rendering BRCA the most common and rapidly expanding form of cancer in females globally. NUF2 has been identified as a prognostic factor in various human cancers, regulating cell apoptosis and proliferation. However, its role in BRCA prognosis has not been clarified. This study explored the role of NUF2 in breast cancer development and prognosis using informatic analysis combined with in vivo intracellular studies. Through the online website TIMER, we evaluated the transcription profile of NUF2 across a variety of different cancer types and found that NUF2 mRNA was highly expressed in BRCA patients. Its transcription level was found to be related to the subtype, pathological stage, and prognosis of BRCA. The R program analysis showed a correlation of NUF2 with cell proliferation and tumor stemness in the BRCA patient samples. Subsequently, the association between the NUF2 expression level and immune cell infiltration was analyzed using the XIANTAO and TIMER tools. The results revealed that NUF2 expression was correlated with the responses of multiple immune cells. Furthermore, we observed the effect of NUF2 expression on tumor stemness in BRCA cell lines in vivo. The experimental results illuminated that the overexpression of NUF2 statistically upregulated the proliferation and tumor stemness ability of the BRCA cell lines MCF-7 and Hs-578T. Meanwhile, the knockdown of NUF2 inhibited the abilities of both cell lines, a finding which was verified by analyzing the subcutaneous tumorigenic ability in nude mice. In summary, this study suggests that NUF2 may play a key role in the development and progression of BRCA by affecting tumor stemness. As a stemness indicator, it has the potential to be one of the markers for the diagnosis of BRCA. breast cancer NUF2 cancer stemness Biology (General) Chemistry Jiapeng Li verfasserin aut Yingjie Zhang verfasserin aut Hao Hu verfasserin aut Fujian Wan verfasserin aut Hang Min verfasserin aut Hao Zhou verfasserin aut Lixing Gu verfasserin aut Xinghua Liao verfasserin aut Jingjiao Zhou verfasserin aut Jun Zhou verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 24(2023), 4, p 4226 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:24 year:2023 number:4, p 4226 https://doi.org/10.3390/ijms24044226 kostenfrei https://doaj.org/article/cd19cac0a5d6483db1981ad7d42d3377 kostenfrei https://www.mdpi.com/1422-0067/24/4/4226 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2023 4, p 4226
spelling	10.3390/ijms24044226 doi (DE-627)DOAJ080253512 (DE-599)DOAJcd19cac0a5d6483db1981ad7d42d3377 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Yang Deng verfasserin aut NUF2 Promotes Breast Cancer Development as a New Tumor Stem Cell Indicator 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Multiple new subtypes of breast cancer (BRCA) are identified in women each year, rendering BRCA the most common and rapidly expanding form of cancer in females globally. NUF2 has been identified as a prognostic factor in various human cancers, regulating cell apoptosis and proliferation. However, its role in BRCA prognosis has not been clarified. This study explored the role of NUF2 in breast cancer development and prognosis using informatic analysis combined with in vivo intracellular studies. Through the online website TIMER, we evaluated the transcription profile of NUF2 across a variety of different cancer types and found that NUF2 mRNA was highly expressed in BRCA patients. Its transcription level was found to be related to the subtype, pathological stage, and prognosis of BRCA. The R program analysis showed a correlation of NUF2 with cell proliferation and tumor stemness in the BRCA patient samples. Subsequently, the association between the NUF2 expression level and immune cell infiltration was analyzed using the XIANTAO and TIMER tools. The results revealed that NUF2 expression was correlated with the responses of multiple immune cells. Furthermore, we observed the effect of NUF2 expression on tumor stemness in BRCA cell lines in vivo. The experimental results illuminated that the overexpression of NUF2 statistically upregulated the proliferation and tumor stemness ability of the BRCA cell lines MCF-7 and Hs-578T. Meanwhile, the knockdown of NUF2 inhibited the abilities of both cell lines, a finding which was verified by analyzing the subcutaneous tumorigenic ability in nude mice. In summary, this study suggests that NUF2 may play a key role in the development and progression of BRCA by affecting tumor stemness. As a stemness indicator, it has the potential to be one of the markers for the diagnosis of BRCA. breast cancer NUF2 cancer stemness Biology (General) Chemistry Jiapeng Li verfasserin aut Yingjie Zhang verfasserin aut Hao Hu verfasserin aut Fujian Wan verfasserin aut Hang Min verfasserin aut Hao Zhou verfasserin aut Lixing Gu verfasserin aut Xinghua Liao verfasserin aut Jingjiao Zhou verfasserin aut Jun Zhou verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 24(2023), 4, p 4226 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:24 year:2023 number:4, p 4226 https://doi.org/10.3390/ijms24044226 kostenfrei https://doaj.org/article/cd19cac0a5d6483db1981ad7d42d3377 kostenfrei https://www.mdpi.com/1422-0067/24/4/4226 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2023 4, p 4226
allfields_unstemmed	10.3390/ijms24044226 doi (DE-627)DOAJ080253512 (DE-599)DOAJcd19cac0a5d6483db1981ad7d42d3377 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Yang Deng verfasserin aut NUF2 Promotes Breast Cancer Development as a New Tumor Stem Cell Indicator 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Multiple new subtypes of breast cancer (BRCA) are identified in women each year, rendering BRCA the most common and rapidly expanding form of cancer in females globally. NUF2 has been identified as a prognostic factor in various human cancers, regulating cell apoptosis and proliferation. However, its role in BRCA prognosis has not been clarified. This study explored the role of NUF2 in breast cancer development and prognosis using informatic analysis combined with in vivo intracellular studies. Through the online website TIMER, we evaluated the transcription profile of NUF2 across a variety of different cancer types and found that NUF2 mRNA was highly expressed in BRCA patients. Its transcription level was found to be related to the subtype, pathological stage, and prognosis of BRCA. The R program analysis showed a correlation of NUF2 with cell proliferation and tumor stemness in the BRCA patient samples. Subsequently, the association between the NUF2 expression level and immune cell infiltration was analyzed using the XIANTAO and TIMER tools. The results revealed that NUF2 expression was correlated with the responses of multiple immune cells. Furthermore, we observed the effect of NUF2 expression on tumor stemness in BRCA cell lines in vivo. The experimental results illuminated that the overexpression of NUF2 statistically upregulated the proliferation and tumor stemness ability of the BRCA cell lines MCF-7 and Hs-578T. Meanwhile, the knockdown of NUF2 inhibited the abilities of both cell lines, a finding which was verified by analyzing the subcutaneous tumorigenic ability in nude mice. In summary, this study suggests that NUF2 may play a key role in the development and progression of BRCA by affecting tumor stemness. As a stemness indicator, it has the potential to be one of the markers for the diagnosis of BRCA. breast cancer NUF2 cancer stemness Biology (General) Chemistry Jiapeng Li verfasserin aut Yingjie Zhang verfasserin aut Hao Hu verfasserin aut Fujian Wan verfasserin aut Hang Min verfasserin aut Hao Zhou verfasserin aut Lixing Gu verfasserin aut Xinghua Liao verfasserin aut Jingjiao Zhou verfasserin aut Jun Zhou verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 24(2023), 4, p 4226 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:24 year:2023 number:4, p 4226 https://doi.org/10.3390/ijms24044226 kostenfrei https://doaj.org/article/cd19cac0a5d6483db1981ad7d42d3377 kostenfrei https://www.mdpi.com/1422-0067/24/4/4226 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2023 4, p 4226
allfieldsGer	10.3390/ijms24044226 doi (DE-627)DOAJ080253512 (DE-599)DOAJcd19cac0a5d6483db1981ad7d42d3377 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Yang Deng verfasserin aut NUF2 Promotes Breast Cancer Development as a New Tumor Stem Cell Indicator 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Multiple new subtypes of breast cancer (BRCA) are identified in women each year, rendering BRCA the most common and rapidly expanding form of cancer in females globally. NUF2 has been identified as a prognostic factor in various human cancers, regulating cell apoptosis and proliferation. However, its role in BRCA prognosis has not been clarified. This study explored the role of NUF2 in breast cancer development and prognosis using informatic analysis combined with in vivo intracellular studies. Through the online website TIMER, we evaluated the transcription profile of NUF2 across a variety of different cancer types and found that NUF2 mRNA was highly expressed in BRCA patients. Its transcription level was found to be related to the subtype, pathological stage, and prognosis of BRCA. The R program analysis showed a correlation of NUF2 with cell proliferation and tumor stemness in the BRCA patient samples. Subsequently, the association between the NUF2 expression level and immune cell infiltration was analyzed using the XIANTAO and TIMER tools. The results revealed that NUF2 expression was correlated with the responses of multiple immune cells. Furthermore, we observed the effect of NUF2 expression on tumor stemness in BRCA cell lines in vivo. The experimental results illuminated that the overexpression of NUF2 statistically upregulated the proliferation and tumor stemness ability of the BRCA cell lines MCF-7 and Hs-578T. Meanwhile, the knockdown of NUF2 inhibited the abilities of both cell lines, a finding which was verified by analyzing the subcutaneous tumorigenic ability in nude mice. In summary, this study suggests that NUF2 may play a key role in the development and progression of BRCA by affecting tumor stemness. As a stemness indicator, it has the potential to be one of the markers for the diagnosis of BRCA. breast cancer NUF2 cancer stemness Biology (General) Chemistry Jiapeng Li verfasserin aut Yingjie Zhang verfasserin aut Hao Hu verfasserin aut Fujian Wan verfasserin aut Hang Min verfasserin aut Hao Zhou verfasserin aut Lixing Gu verfasserin aut Xinghua Liao verfasserin aut Jingjiao Zhou verfasserin aut Jun Zhou verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 24(2023), 4, p 4226 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:24 year:2023 number:4, p 4226 https://doi.org/10.3390/ijms24044226 kostenfrei https://doaj.org/article/cd19cac0a5d6483db1981ad7d42d3377 kostenfrei https://www.mdpi.com/1422-0067/24/4/4226 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2023 4, p 4226
allfieldsSound	10.3390/ijms24044226 doi (DE-627)DOAJ080253512 (DE-599)DOAJcd19cac0a5d6483db1981ad7d42d3377 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Yang Deng verfasserin aut NUF2 Promotes Breast Cancer Development as a New Tumor Stem Cell Indicator 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Multiple new subtypes of breast cancer (BRCA) are identified in women each year, rendering BRCA the most common and rapidly expanding form of cancer in females globally. NUF2 has been identified as a prognostic factor in various human cancers, regulating cell apoptosis and proliferation. However, its role in BRCA prognosis has not been clarified. This study explored the role of NUF2 in breast cancer development and prognosis using informatic analysis combined with in vivo intracellular studies. Through the online website TIMER, we evaluated the transcription profile of NUF2 across a variety of different cancer types and found that NUF2 mRNA was highly expressed in BRCA patients. Its transcription level was found to be related to the subtype, pathological stage, and prognosis of BRCA. The R program analysis showed a correlation of NUF2 with cell proliferation and tumor stemness in the BRCA patient samples. Subsequently, the association between the NUF2 expression level and immune cell infiltration was analyzed using the XIANTAO and TIMER tools. The results revealed that NUF2 expression was correlated with the responses of multiple immune cells. Furthermore, we observed the effect of NUF2 expression on tumor stemness in BRCA cell lines in vivo. The experimental results illuminated that the overexpression of NUF2 statistically upregulated the proliferation and tumor stemness ability of the BRCA cell lines MCF-7 and Hs-578T. Meanwhile, the knockdown of NUF2 inhibited the abilities of both cell lines, a finding which was verified by analyzing the subcutaneous tumorigenic ability in nude mice. In summary, this study suggests that NUF2 may play a key role in the development and progression of BRCA by affecting tumor stemness. As a stemness indicator, it has the potential to be one of the markers for the diagnosis of BRCA. breast cancer NUF2 cancer stemness Biology (General) Chemistry Jiapeng Li verfasserin aut Yingjie Zhang verfasserin aut Hao Hu verfasserin aut Fujian Wan verfasserin aut Hang Min verfasserin aut Hao Zhou verfasserin aut Lixing Gu verfasserin aut Xinghua Liao verfasserin aut Jingjiao Zhou verfasserin aut Jun Zhou verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 24(2023), 4, p 4226 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:24 year:2023 number:4, p 4226 https://doi.org/10.3390/ijms24044226 kostenfrei https://doaj.org/article/cd19cac0a5d6483db1981ad7d42d3377 kostenfrei https://www.mdpi.com/1422-0067/24/4/4226 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2023 4, p 4226
language	English
source	In International Journal of Molecular Sciences 24(2023), 4, p 4226 volume:24 year:2023 number:4, p 4226
sourceStr	In International Journal of Molecular Sciences 24(2023), 4, p 4226 volume:24 year:2023 number:4, p 4226
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	breast cancer NUF2 cancer stemness Biology (General) Chemistry
isfreeaccess_bool	true
container_title	International Journal of Molecular Sciences
authorswithroles_txt_mv	Yang Deng @@aut@@ Jiapeng Li @@aut@@ Yingjie Zhang @@aut@@ Hao Hu @@aut@@ Fujian Wan @@aut@@ Hang Min @@aut@@ Hao Zhou @@aut@@ Lixing Gu @@aut@@ Xinghua Liao @@aut@@ Jingjiao Zhou @@aut@@ Jun Zhou @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
hierarchy_top_id	316340715
id	DOAJ080253512
language_de	englisch
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callnumber-first	Q - Science
author	Yang Deng
spellingShingle	Yang Deng misc QH301-705.5 misc QD1-999 misc breast cancer misc NUF2 misc cancer stemness misc Biology (General) misc Chemistry NUF2 Promotes Breast Cancer Development as a New Tumor Stem Cell Indicator
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topic_title	QH301-705.5 QD1-999 NUF2 Promotes Breast Cancer Development as a New Tumor Stem Cell Indicator breast cancer NUF2 cancer stemness
topic	misc QH301-705.5 misc QD1-999 misc breast cancer misc NUF2 misc cancer stemness misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc breast cancer misc NUF2 misc cancer stemness misc Biology (General) misc Chemistry
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title	NUF2 Promotes Breast Cancer Development as a New Tumor Stem Cell Indicator
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title_full	NUF2 Promotes Breast Cancer Development as a New Tumor Stem Cell Indicator
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author_browse	Yang Deng Jiapeng Li Yingjie Zhang Hao Hu Fujian Wan Hang Min Hao Zhou Lixing Gu Xinghua Liao Jingjiao Zhou Jun Zhou
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title_sort	nuf2 promotes breast cancer development as a new tumor stem cell indicator
callnumber	QH301-705.5
title_auth	NUF2 Promotes Breast Cancer Development as a New Tumor Stem Cell Indicator
abstract	Multiple new subtypes of breast cancer (BRCA) are identified in women each year, rendering BRCA the most common and rapidly expanding form of cancer in females globally. NUF2 has been identified as a prognostic factor in various human cancers, regulating cell apoptosis and proliferation. However, its role in BRCA prognosis has not been clarified. This study explored the role of NUF2 in breast cancer development and prognosis using informatic analysis combined with in vivo intracellular studies. Through the online website TIMER, we evaluated the transcription profile of NUF2 across a variety of different cancer types and found that NUF2 mRNA was highly expressed in BRCA patients. Its transcription level was found to be related to the subtype, pathological stage, and prognosis of BRCA. The R program analysis showed a correlation of NUF2 with cell proliferation and tumor stemness in the BRCA patient samples. Subsequently, the association between the NUF2 expression level and immune cell infiltration was analyzed using the XIANTAO and TIMER tools. The results revealed that NUF2 expression was correlated with the responses of multiple immune cells. Furthermore, we observed the effect of NUF2 expression on tumor stemness in BRCA cell lines in vivo. The experimental results illuminated that the overexpression of NUF2 statistically upregulated the proliferation and tumor stemness ability of the BRCA cell lines MCF-7 and Hs-578T. Meanwhile, the knockdown of NUF2 inhibited the abilities of both cell lines, a finding which was verified by analyzing the subcutaneous tumorigenic ability in nude mice. In summary, this study suggests that NUF2 may play a key role in the development and progression of BRCA by affecting tumor stemness. As a stemness indicator, it has the potential to be one of the markers for the diagnosis of BRCA.
abstractGer	Multiple new subtypes of breast cancer (BRCA) are identified in women each year, rendering BRCA the most common and rapidly expanding form of cancer in females globally. NUF2 has been identified as a prognostic factor in various human cancers, regulating cell apoptosis and proliferation. However, its role in BRCA prognosis has not been clarified. This study explored the role of NUF2 in breast cancer development and prognosis using informatic analysis combined with in vivo intracellular studies. Through the online website TIMER, we evaluated the transcription profile of NUF2 across a variety of different cancer types and found that NUF2 mRNA was highly expressed in BRCA patients. Its transcription level was found to be related to the subtype, pathological stage, and prognosis of BRCA. The R program analysis showed a correlation of NUF2 with cell proliferation and tumor stemness in the BRCA patient samples. Subsequently, the association between the NUF2 expression level and immune cell infiltration was analyzed using the XIANTAO and TIMER tools. The results revealed that NUF2 expression was correlated with the responses of multiple immune cells. Furthermore, we observed the effect of NUF2 expression on tumor stemness in BRCA cell lines in vivo. The experimental results illuminated that the overexpression of NUF2 statistically upregulated the proliferation and tumor stemness ability of the BRCA cell lines MCF-7 and Hs-578T. Meanwhile, the knockdown of NUF2 inhibited the abilities of both cell lines, a finding which was verified by analyzing the subcutaneous tumorigenic ability in nude mice. In summary, this study suggests that NUF2 may play a key role in the development and progression of BRCA by affecting tumor stemness. As a stemness indicator, it has the potential to be one of the markers for the diagnosis of BRCA.
abstract_unstemmed	Multiple new subtypes of breast cancer (BRCA) are identified in women each year, rendering BRCA the most common and rapidly expanding form of cancer in females globally. NUF2 has been identified as a prognostic factor in various human cancers, regulating cell apoptosis and proliferation. However, its role in BRCA prognosis has not been clarified. This study explored the role of NUF2 in breast cancer development and prognosis using informatic analysis combined with in vivo intracellular studies. Through the online website TIMER, we evaluated the transcription profile of NUF2 across a variety of different cancer types and found that NUF2 mRNA was highly expressed in BRCA patients. Its transcription level was found to be related to the subtype, pathological stage, and prognosis of BRCA. The R program analysis showed a correlation of NUF2 with cell proliferation and tumor stemness in the BRCA patient samples. Subsequently, the association between the NUF2 expression level and immune cell infiltration was analyzed using the XIANTAO and TIMER tools. The results revealed that NUF2 expression was correlated with the responses of multiple immune cells. Furthermore, we observed the effect of NUF2 expression on tumor stemness in BRCA cell lines in vivo. The experimental results illuminated that the overexpression of NUF2 statistically upregulated the proliferation and tumor stemness ability of the BRCA cell lines MCF-7 and Hs-578T. Meanwhile, the knockdown of NUF2 inhibited the abilities of both cell lines, a finding which was verified by analyzing the subcutaneous tumorigenic ability in nude mice. In summary, this study suggests that NUF2 may play a key role in the development and progression of BRCA by affecting tumor stemness. As a stemness indicator, it has the potential to be one of the markers for the diagnosis of BRCA.
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title_short	NUF2 Promotes Breast Cancer Development as a New Tumor Stem Cell Indicator
url	https://doi.org/10.3390/ijms24044226 https://doaj.org/article/cd19cac0a5d6483db1981ad7d42d3377 https://www.mdpi.com/1422-0067/24/4/4226 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067
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