The Study of a Novel Paeoniflorin-Converting Enzyme from <i<Cunninghamella blakesleeana</i<

Paeoniflorin is a glycoside compound found in <i<Paeonia lactiflora</i< Pall that is used in traditional herbal medicine and shows various protective effects on the cardio-cerebral vascular system. It has been reported that the pharmacological effects of paeoniflorin might be generated b...
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Autor*in:	Yiheng Ye [verfasserIn] Hairun Pei [verfasserIn] Xueli Cao [verfasserIn] Xueying Liu [verfasserIn] Zhanghan Li [verfasserIn] Biying Wang [verfasserIn] Yan Pan [verfasserIn] Jimin Zheng [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	biotransformation paeoniflorin

Übergeordnetes Werk:	In: Molecules - MDPI AG, 2003, 28(2023), 3, p 1289
Übergeordnetes Werk:	volume:28 ; year:2023 ; number:3, p 1289

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/molecules28031289

Katalog-ID:	DOAJ080613594

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allfields	10.3390/molecules28031289 doi (DE-627)DOAJ080613594 (DE-599)DOAJ95e6ca27c8a8440d814178193e719a21 DE-627 ger DE-627 rakwb eng QD241-441 Yiheng Ye verfasserin aut The Study of a Novel Paeoniflorin-Converting Enzyme from <i<Cunninghamella blakesleeana</i< 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Paeoniflorin is a glycoside compound found in <i<Paeonia lactiflora</i< Pall that is used in traditional herbal medicine and shows various protective effects on the cardio-cerebral vascular system. It has been reported that the pharmacological effects of paeoniflorin might be generated by its metabolites. However, the bioavailability of paeoniflorin by oral administration is low, which greatly limits its clinical application. In this paper, a paeoniflorin-converting enzyme gene (G6046, GenBank accession numbers: OP856858) from <i<Cunninghamella blakesleeana</i< (AS 3.970) was identified by comparative analysis between MS analysis and transcriptomics. The expression, purification, enzyme activity, and structure of the conversion products produced by this paeoniflorin-converting enzyme were studied. The optimal conditions for the enzymatic activity were found to be pH 9, 45 °C, resulting in a specific enzyme activity of 14.56 U/mg. The products were separated and purified by high-performance counter-current chromatography (HPCCC). Two main components were isolated and identified, 2-amino-2-p-hydroxymethyl-methyl alcohol-benzoate (tirs-benzoate) and 1-benzoyloxy-2,3-propanediol (1-benzoyloxypropane-2,3-diol), via UPLC-Q-TOF-MS and NMR. Additionally, paeoniflorin demonstrated the ability to metabolize into benzoic acid via G6046 enzyme, which might exert antidepressant effects through the blood–brain barrier into the brain. biotransformation <i<Cunninghamella blakesleeana</i< paeoniflorin Organic chemistry Hairun Pei verfasserin aut Xueli Cao verfasserin aut Xueying Liu verfasserin aut Zhanghan Li verfasserin aut Biying Wang verfasserin aut Yan Pan verfasserin aut Jimin Zheng verfasserin aut In Molecules MDPI AG, 2003 28(2023), 3, p 1289 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:28 year:2023 number:3, p 1289 https://doi.org/10.3390/molecules28031289 kostenfrei https://doaj.org/article/95e6ca27c8a8440d814178193e719a21 kostenfrei https://www.mdpi.com/1420-3049/28/3/1289 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 28 2023 3, p 1289
spelling	10.3390/molecules28031289 doi (DE-627)DOAJ080613594 (DE-599)DOAJ95e6ca27c8a8440d814178193e719a21 DE-627 ger DE-627 rakwb eng QD241-441 Yiheng Ye verfasserin aut The Study of a Novel Paeoniflorin-Converting Enzyme from <i<Cunninghamella blakesleeana</i< 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Paeoniflorin is a glycoside compound found in <i<Paeonia lactiflora</i< Pall that is used in traditional herbal medicine and shows various protective effects on the cardio-cerebral vascular system. It has been reported that the pharmacological effects of paeoniflorin might be generated by its metabolites. However, the bioavailability of paeoniflorin by oral administration is low, which greatly limits its clinical application. In this paper, a paeoniflorin-converting enzyme gene (G6046, GenBank accession numbers: OP856858) from <i<Cunninghamella blakesleeana</i< (AS 3.970) was identified by comparative analysis between MS analysis and transcriptomics. The expression, purification, enzyme activity, and structure of the conversion products produced by this paeoniflorin-converting enzyme were studied. The optimal conditions for the enzymatic activity were found to be pH 9, 45 °C, resulting in a specific enzyme activity of 14.56 U/mg. The products were separated and purified by high-performance counter-current chromatography (HPCCC). Two main components were isolated and identified, 2-amino-2-p-hydroxymethyl-methyl alcohol-benzoate (tirs-benzoate) and 1-benzoyloxy-2,3-propanediol (1-benzoyloxypropane-2,3-diol), via UPLC-Q-TOF-MS and NMR. Additionally, paeoniflorin demonstrated the ability to metabolize into benzoic acid via G6046 enzyme, which might exert antidepressant effects through the blood–brain barrier into the brain. biotransformation <i<Cunninghamella blakesleeana</i< paeoniflorin Organic chemistry Hairun Pei verfasserin aut Xueli Cao verfasserin aut Xueying Liu verfasserin aut Zhanghan Li verfasserin aut Biying Wang verfasserin aut Yan Pan verfasserin aut Jimin Zheng verfasserin aut In Molecules MDPI AG, 2003 28(2023), 3, p 1289 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:28 year:2023 number:3, p 1289 https://doi.org/10.3390/molecules28031289 kostenfrei https://doaj.org/article/95e6ca27c8a8440d814178193e719a21 kostenfrei https://www.mdpi.com/1420-3049/28/3/1289 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 28 2023 3, p 1289
allfields_unstemmed	10.3390/molecules28031289 doi (DE-627)DOAJ080613594 (DE-599)DOAJ95e6ca27c8a8440d814178193e719a21 DE-627 ger DE-627 rakwb eng QD241-441 Yiheng Ye verfasserin aut The Study of a Novel Paeoniflorin-Converting Enzyme from <i<Cunninghamella blakesleeana</i< 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Paeoniflorin is a glycoside compound found in <i<Paeonia lactiflora</i< Pall that is used in traditional herbal medicine and shows various protective effects on the cardio-cerebral vascular system. It has been reported that the pharmacological effects of paeoniflorin might be generated by its metabolites. However, the bioavailability of paeoniflorin by oral administration is low, which greatly limits its clinical application. In this paper, a paeoniflorin-converting enzyme gene (G6046, GenBank accession numbers: OP856858) from <i<Cunninghamella blakesleeana</i< (AS 3.970) was identified by comparative analysis between MS analysis and transcriptomics. The expression, purification, enzyme activity, and structure of the conversion products produced by this paeoniflorin-converting enzyme were studied. The optimal conditions for the enzymatic activity were found to be pH 9, 45 °C, resulting in a specific enzyme activity of 14.56 U/mg. The products were separated and purified by high-performance counter-current chromatography (HPCCC). Two main components were isolated and identified, 2-amino-2-p-hydroxymethyl-methyl alcohol-benzoate (tirs-benzoate) and 1-benzoyloxy-2,3-propanediol (1-benzoyloxypropane-2,3-diol), via UPLC-Q-TOF-MS and NMR. Additionally, paeoniflorin demonstrated the ability to metabolize into benzoic acid via G6046 enzyme, which might exert antidepressant effects through the blood–brain barrier into the brain. biotransformation <i<Cunninghamella blakesleeana</i< paeoniflorin Organic chemistry Hairun Pei verfasserin aut Xueli Cao verfasserin aut Xueying Liu verfasserin aut Zhanghan Li verfasserin aut Biying Wang verfasserin aut Yan Pan verfasserin aut Jimin Zheng verfasserin aut In Molecules MDPI AG, 2003 28(2023), 3, p 1289 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:28 year:2023 number:3, p 1289 https://doi.org/10.3390/molecules28031289 kostenfrei https://doaj.org/article/95e6ca27c8a8440d814178193e719a21 kostenfrei https://www.mdpi.com/1420-3049/28/3/1289 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 28 2023 3, p 1289
allfieldsGer	10.3390/molecules28031289 doi (DE-627)DOAJ080613594 (DE-599)DOAJ95e6ca27c8a8440d814178193e719a21 DE-627 ger DE-627 rakwb eng QD241-441 Yiheng Ye verfasserin aut The Study of a Novel Paeoniflorin-Converting Enzyme from <i<Cunninghamella blakesleeana</i< 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Paeoniflorin is a glycoside compound found in <i<Paeonia lactiflora</i< Pall that is used in traditional herbal medicine and shows various protective effects on the cardio-cerebral vascular system. It has been reported that the pharmacological effects of paeoniflorin might be generated by its metabolites. However, the bioavailability of paeoniflorin by oral administration is low, which greatly limits its clinical application. In this paper, a paeoniflorin-converting enzyme gene (G6046, GenBank accession numbers: OP856858) from <i<Cunninghamella blakesleeana</i< (AS 3.970) was identified by comparative analysis between MS analysis and transcriptomics. The expression, purification, enzyme activity, and structure of the conversion products produced by this paeoniflorin-converting enzyme were studied. The optimal conditions for the enzymatic activity were found to be pH 9, 45 °C, resulting in a specific enzyme activity of 14.56 U/mg. The products were separated and purified by high-performance counter-current chromatography (HPCCC). Two main components were isolated and identified, 2-amino-2-p-hydroxymethyl-methyl alcohol-benzoate (tirs-benzoate) and 1-benzoyloxy-2,3-propanediol (1-benzoyloxypropane-2,3-diol), via UPLC-Q-TOF-MS and NMR. Additionally, paeoniflorin demonstrated the ability to metabolize into benzoic acid via G6046 enzyme, which might exert antidepressant effects through the blood–brain barrier into the brain. biotransformation <i<Cunninghamella blakesleeana</i< paeoniflorin Organic chemistry Hairun Pei verfasserin aut Xueli Cao verfasserin aut Xueying Liu verfasserin aut Zhanghan Li verfasserin aut Biying Wang verfasserin aut Yan Pan verfasserin aut Jimin Zheng verfasserin aut In Molecules MDPI AG, 2003 28(2023), 3, p 1289 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:28 year:2023 number:3, p 1289 https://doi.org/10.3390/molecules28031289 kostenfrei https://doaj.org/article/95e6ca27c8a8440d814178193e719a21 kostenfrei https://www.mdpi.com/1420-3049/28/3/1289 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 28 2023 3, p 1289
allfieldsSound	10.3390/molecules28031289 doi (DE-627)DOAJ080613594 (DE-599)DOAJ95e6ca27c8a8440d814178193e719a21 DE-627 ger DE-627 rakwb eng QD241-441 Yiheng Ye verfasserin aut The Study of a Novel Paeoniflorin-Converting Enzyme from <i<Cunninghamella blakesleeana</i< 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Paeoniflorin is a glycoside compound found in <i<Paeonia lactiflora</i< Pall that is used in traditional herbal medicine and shows various protective effects on the cardio-cerebral vascular system. It has been reported that the pharmacological effects of paeoniflorin might be generated by its metabolites. However, the bioavailability of paeoniflorin by oral administration is low, which greatly limits its clinical application. In this paper, a paeoniflorin-converting enzyme gene (G6046, GenBank accession numbers: OP856858) from <i<Cunninghamella blakesleeana</i< (AS 3.970) was identified by comparative analysis between MS analysis and transcriptomics. The expression, purification, enzyme activity, and structure of the conversion products produced by this paeoniflorin-converting enzyme were studied. The optimal conditions for the enzymatic activity were found to be pH 9, 45 °C, resulting in a specific enzyme activity of 14.56 U/mg. The products were separated and purified by high-performance counter-current chromatography (HPCCC). Two main components were isolated and identified, 2-amino-2-p-hydroxymethyl-methyl alcohol-benzoate (tirs-benzoate) and 1-benzoyloxy-2,3-propanediol (1-benzoyloxypropane-2,3-diol), via UPLC-Q-TOF-MS and NMR. Additionally, paeoniflorin demonstrated the ability to metabolize into benzoic acid via G6046 enzyme, which might exert antidepressant effects through the blood–brain barrier into the brain. biotransformation <i<Cunninghamella blakesleeana</i< paeoniflorin Organic chemistry Hairun Pei verfasserin aut Xueli Cao verfasserin aut Xueying Liu verfasserin aut Zhanghan Li verfasserin aut Biying Wang verfasserin aut Yan Pan verfasserin aut Jimin Zheng verfasserin aut In Molecules MDPI AG, 2003 28(2023), 3, p 1289 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:28 year:2023 number:3, p 1289 https://doi.org/10.3390/molecules28031289 kostenfrei https://doaj.org/article/95e6ca27c8a8440d814178193e719a21 kostenfrei https://www.mdpi.com/1420-3049/28/3/1289 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 28 2023 3, p 1289
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callnumber-first	Q - Science
author	Yiheng Ye
spellingShingle	Yiheng Ye misc QD241-441 misc biotransformation misc <i<Cunninghamella blakesleeana</i< misc paeoniflorin misc Organic chemistry The Study of a Novel Paeoniflorin-Converting Enzyme from <i<Cunninghamella blakesleeana</i<
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topic_title	QD241-441 The Study of a Novel Paeoniflorin-Converting Enzyme from <i<Cunninghamella blakesleeana</i< biotransformation <i<Cunninghamella blakesleeana</i< paeoniflorin
topic	misc QD241-441 misc biotransformation misc <i<Cunninghamella blakesleeana</i< misc paeoniflorin misc Organic chemistry
topic_unstemmed	misc QD241-441 misc biotransformation misc <i<Cunninghamella blakesleeana</i< misc paeoniflorin misc Organic chemistry
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title	The Study of a Novel Paeoniflorin-Converting Enzyme from <i<Cunninghamella blakesleeana</i<
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title_full	The Study of a Novel Paeoniflorin-Converting Enzyme from <i<Cunninghamella blakesleeana</i<
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title_sort	study of a novel paeoniflorin-converting enzyme from <i<cunninghamella blakesleeana</i<
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title_auth	The Study of a Novel Paeoniflorin-Converting Enzyme from <i<Cunninghamella blakesleeana</i<
abstract	Paeoniflorin is a glycoside compound found in <i<Paeonia lactiflora</i< Pall that is used in traditional herbal medicine and shows various protective effects on the cardio-cerebral vascular system. It has been reported that the pharmacological effects of paeoniflorin might be generated by its metabolites. However, the bioavailability of paeoniflorin by oral administration is low, which greatly limits its clinical application. In this paper, a paeoniflorin-converting enzyme gene (G6046, GenBank accession numbers: OP856858) from <i<Cunninghamella blakesleeana</i< (AS 3.970) was identified by comparative analysis between MS analysis and transcriptomics. The expression, purification, enzyme activity, and structure of the conversion products produced by this paeoniflorin-converting enzyme were studied. The optimal conditions for the enzymatic activity were found to be pH 9, 45 °C, resulting in a specific enzyme activity of 14.56 U/mg. The products were separated and purified by high-performance counter-current chromatography (HPCCC). Two main components were isolated and identified, 2-amino-2-p-hydroxymethyl-methyl alcohol-benzoate (tirs-benzoate) and 1-benzoyloxy-2,3-propanediol (1-benzoyloxypropane-2,3-diol), via UPLC-Q-TOF-MS and NMR. Additionally, paeoniflorin demonstrated the ability to metabolize into benzoic acid via G6046 enzyme, which might exert antidepressant effects through the blood–brain barrier into the brain.
abstractGer	Paeoniflorin is a glycoside compound found in <i<Paeonia lactiflora</i< Pall that is used in traditional herbal medicine and shows various protective effects on the cardio-cerebral vascular system. It has been reported that the pharmacological effects of paeoniflorin might be generated by its metabolites. However, the bioavailability of paeoniflorin by oral administration is low, which greatly limits its clinical application. In this paper, a paeoniflorin-converting enzyme gene (G6046, GenBank accession numbers: OP856858) from <i<Cunninghamella blakesleeana</i< (AS 3.970) was identified by comparative analysis between MS analysis and transcriptomics. The expression, purification, enzyme activity, and structure of the conversion products produced by this paeoniflorin-converting enzyme were studied. The optimal conditions for the enzymatic activity were found to be pH 9, 45 °C, resulting in a specific enzyme activity of 14.56 U/mg. The products were separated and purified by high-performance counter-current chromatography (HPCCC). Two main components were isolated and identified, 2-amino-2-p-hydroxymethyl-methyl alcohol-benzoate (tirs-benzoate) and 1-benzoyloxy-2,3-propanediol (1-benzoyloxypropane-2,3-diol), via UPLC-Q-TOF-MS and NMR. Additionally, paeoniflorin demonstrated the ability to metabolize into benzoic acid via G6046 enzyme, which might exert antidepressant effects through the blood–brain barrier into the brain.
abstract_unstemmed	Paeoniflorin is a glycoside compound found in <i<Paeonia lactiflora</i< Pall that is used in traditional herbal medicine and shows various protective effects on the cardio-cerebral vascular system. It has been reported that the pharmacological effects of paeoniflorin might be generated by its metabolites. However, the bioavailability of paeoniflorin by oral administration is low, which greatly limits its clinical application. In this paper, a paeoniflorin-converting enzyme gene (G6046, GenBank accession numbers: OP856858) from <i<Cunninghamella blakesleeana</i< (AS 3.970) was identified by comparative analysis between MS analysis and transcriptomics. The expression, purification, enzyme activity, and structure of the conversion products produced by this paeoniflorin-converting enzyme were studied. The optimal conditions for the enzymatic activity were found to be pH 9, 45 °C, resulting in a specific enzyme activity of 14.56 U/mg. The products were separated and purified by high-performance counter-current chromatography (HPCCC). Two main components were isolated and identified, 2-amino-2-p-hydroxymethyl-methyl alcohol-benzoate (tirs-benzoate) and 1-benzoyloxy-2,3-propanediol (1-benzoyloxypropane-2,3-diol), via UPLC-Q-TOF-MS and NMR. Additionally, paeoniflorin demonstrated the ability to metabolize into benzoic acid via G6046 enzyme, which might exert antidepressant effects through the blood–brain barrier into the brain.
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title_short	The Study of a Novel Paeoniflorin-Converting Enzyme from <i<Cunninghamella blakesleeana</i<
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Two main components were isolated and identified, 2-amino-2-p-hydroxymethyl-methyl alcohol-benzoate (tirs-benzoate) and 1-benzoyloxy-2,3-propanediol (1-benzoyloxypropane-2,3-diol), via UPLC-Q-TOF-MS and NMR. 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The Study of a Novel Paeoniflorin-Converting Enzyme from <i<Cunninghamella blakesleeana</i<

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