Determination of Genetic Mutation Profile of drpr Gene in Drosophila melanogaster using High-Resolution Melting Analysis

Genotype determination of experimental animals is generally conducted using sequencing methods that require expensive cost as well as experience and special equipment. This study aimed to determine the presence of drpr gene mutation in Drosophila melanogaster using coupled real time PCR-High Resolut...
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Autor*in:	Ahmad Mu'arif [verfasserIn] Rudi Arfiansyah [verfasserIn] Tri Puspita Roska [verfasserIn] Herlina Rante [verfasserIn] Firzan Nainu [verfasserIn]

Format:	E-Artikel
Sprache:	Indonesisch

Erschienen:	2022

Schlagwörter:	drpr high resolution melting genetic mutation drosophila melanogaster

Übergeordnetes Werk:	In: Jurnal Biota - Universitas Islam Negeri Raden Fatah Palembang, 2017, 8(2022), 1, Seite 11-18
Übergeordnetes Werk:	volume:8 ; year:2022 ; number:1 ; pages:11-18

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.19109/Biota.v8i1.7556

Katalog-ID:	DOAJ080811310

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spelling	10.19109/Biota.v8i1.7556 doi (DE-627)DOAJ080811310 (DE-599)DOAJ2fd7d4ee99f14a0ebd58b4dae78e36ec DE-627 ger DE-627 rakwb ind QH301-705.5 Ahmad Mu'arif verfasserin aut Determination of Genetic Mutation Profile of drpr Gene in Drosophila melanogaster using High-Resolution Melting Analysis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Genotype determination of experimental animals is generally conducted using sequencing methods that require expensive cost as well as experience and special equipment. This study aimed to determine the presence of drpr gene mutation in Drosophila melanogaster using coupled real time PCR-High Resolution Melting (real time PCR-HRM) as an alternative method. Two types of fly samples, w1118 and drprΔ5 were used as wildtype control and mutant genotype, respectively. The DNA from twenty of each w1118 and mutant drprΔ5 flies were isolated and amplified using real time PCR. The generated amplicons were then further processed by HRM method at the temperature of 60-95°C. This study demonstrated that the real time PCR-HRM method could distinguish wildtype control w1118 and mutant drprΔ5 based on the HRM data with the confidence level was more than 90%. Therefore, this study provides an evidence that real time PCR-HRM method might be beneficial to screen the mutant genotype from its wildtype counterpart based on differences in the melting temperatures due to changes at nucleotide base level drpr high resolution melting genetic mutation drosophila melanogaster Biology (General) Rudi Arfiansyah verfasserin aut Tri Puspita Roska verfasserin aut Herlina Rante verfasserin aut Firzan Nainu verfasserin aut In Jurnal Biota Universitas Islam Negeri Raden Fatah Palembang, 2017 8(2022), 1, Seite 11-18 (DE-627)1760620599 24607746 nnns volume:8 year:2022 number:1 pages:11-18 https://doi.org/10.19109/Biota.v8i1.7556 kostenfrei https://doaj.org/article/2fd7d4ee99f14a0ebd58b4dae78e36ec kostenfrei http://jurnal.radenfatah.ac.id/index.php/biota/article/view/7556 kostenfrei https://doaj.org/toc/2528-262X Journal toc kostenfrei https://doaj.org/toc/2460-7746 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2022 1 11-18
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language	Indonesian
source	In Jurnal Biota 8(2022), 1, Seite 11-18 volume:8 year:2022 number:1 pages:11-18
sourceStr	In Jurnal Biota 8(2022), 1, Seite 11-18 volume:8 year:2022 number:1 pages:11-18
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topic_facet	drpr high resolution melting genetic mutation drosophila melanogaster Biology (General)
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authorswithroles_txt_mv	Ahmad Mu'arif @@aut@@ Rudi Arfiansyah @@aut@@ Tri Puspita Roska @@aut@@ Herlina Rante @@aut@@ Firzan Nainu @@aut@@
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callnumber-first	Q - Science
author	Ahmad Mu'arif
spellingShingle	Ahmad Mu'arif misc QH301-705.5 misc drpr misc high resolution melting misc genetic mutation misc drosophila melanogaster misc Biology (General) Determination of Genetic Mutation Profile of drpr Gene in Drosophila melanogaster using High-Resolution Melting Analysis
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topic_title	QH301-705.5 Determination of Genetic Mutation Profile of drpr Gene in Drosophila melanogaster using High-Resolution Melting Analysis drpr high resolution melting genetic mutation drosophila melanogaster
topic	misc QH301-705.5 misc drpr misc high resolution melting misc genetic mutation misc drosophila melanogaster misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc drpr misc high resolution melting misc genetic mutation misc drosophila melanogaster misc Biology (General)
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title	Determination of Genetic Mutation Profile of drpr Gene in Drosophila melanogaster using High-Resolution Melting Analysis
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title_full	Determination of Genetic Mutation Profile of drpr Gene in Drosophila melanogaster using High-Resolution Melting Analysis
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title_sort	determination of genetic mutation profile of drpr gene in drosophila melanogaster using high-resolution melting analysis
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title_auth	Determination of Genetic Mutation Profile of drpr Gene in Drosophila melanogaster using High-Resolution Melting Analysis
abstract	Genotype determination of experimental animals is generally conducted using sequencing methods that require expensive cost as well as experience and special equipment. This study aimed to determine the presence of drpr gene mutation in Drosophila melanogaster using coupled real time PCR-High Resolution Melting (real time PCR-HRM) as an alternative method. Two types of fly samples, w1118 and drprΔ5 were used as wildtype control and mutant genotype, respectively. The DNA from twenty of each w1118 and mutant drprΔ5 flies were isolated and amplified using real time PCR. The generated amplicons were then further processed by HRM method at the temperature of 60-95°C. This study demonstrated that the real time PCR-HRM method could distinguish wildtype control w1118 and mutant drprΔ5 based on the HRM data with the confidence level was more than 90%. Therefore, this study provides an evidence that real time PCR-HRM method might be beneficial to screen the mutant genotype from its wildtype counterpart based on differences in the melting temperatures due to changes at nucleotide base level
abstractGer	Genotype determination of experimental animals is generally conducted using sequencing methods that require expensive cost as well as experience and special equipment. This study aimed to determine the presence of drpr gene mutation in Drosophila melanogaster using coupled real time PCR-High Resolution Melting (real time PCR-HRM) as an alternative method. Two types of fly samples, w1118 and drprΔ5 were used as wildtype control and mutant genotype, respectively. The DNA from twenty of each w1118 and mutant drprΔ5 flies were isolated and amplified using real time PCR. The generated amplicons were then further processed by HRM method at the temperature of 60-95°C. This study demonstrated that the real time PCR-HRM method could distinguish wildtype control w1118 and mutant drprΔ5 based on the HRM data with the confidence level was more than 90%. Therefore, this study provides an evidence that real time PCR-HRM method might be beneficial to screen the mutant genotype from its wildtype counterpart based on differences in the melting temperatures due to changes at nucleotide base level
abstract_unstemmed	Genotype determination of experimental animals is generally conducted using sequencing methods that require expensive cost as well as experience and special equipment. This study aimed to determine the presence of drpr gene mutation in Drosophila melanogaster using coupled real time PCR-High Resolution Melting (real time PCR-HRM) as an alternative method. Two types of fly samples, w1118 and drprΔ5 were used as wildtype control and mutant genotype, respectively. The DNA from twenty of each w1118 and mutant drprΔ5 flies were isolated and amplified using real time PCR. The generated amplicons were then further processed by HRM method at the temperature of 60-95°C. This study demonstrated that the real time PCR-HRM method could distinguish wildtype control w1118 and mutant drprΔ5 based on the HRM data with the confidence level was more than 90%. Therefore, this study provides an evidence that real time PCR-HRM method might be beneficial to screen the mutant genotype from its wildtype counterpart based on differences in the melting temperatures due to changes at nucleotide base level
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title_short	Determination of Genetic Mutation Profile of drpr Gene in Drosophila melanogaster using High-Resolution Melting Analysis
url	https://doi.org/10.19109/Biota.v8i1.7556 https://doaj.org/article/2fd7d4ee99f14a0ebd58b4dae78e36ec http://jurnal.radenfatah.ac.id/index.php/biota/article/view/7556 https://doaj.org/toc/2528-262X https://doaj.org/toc/2460-7746
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up_date	2024-07-03T16:41:28.203Z
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Determination of Genetic Mutation Profile of drpr Gene in Drosophila melanogaster using High-Resolution Melting Analysis

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