Characterization of coccidiosis and evaluation of suggestive cases of subclinical necrotic enteritis in broilers

ABSTRACT: This study performed the characterization of coccidiosis in broilers and evaluated the occurrence of suggestive cases of necrotic enteritis (NE), seeking if there is an association between the diseases in Brazilian flocks. Two hundred and fifty-six birds from 32 flocks were evaluated. Macr...
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Autor*in:	Fabio Santiani [verfasserIn] Rodrigo Otávio S. Silva [verfasserIn] Carlos Augusto de Oliveira Júnior [verfasserIn] Jéssica Aline Withoeft [verfasserIn] Thierry G. Cristo [verfasserIn] Leonardo S. Costa [verfasserIn] Taís Gaspar [verfasserIn] Renata A. Casagrande [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch ; Portugiesisch

Erschienen:	2023

Schlagwörter:	Coccidiosis necrotic enteritis broilers eimeriosis clostridiosis genotyping intestinal disease

Übergeordnetes Werk:	In: Pesquisa Veterinária Brasileira - Colégio Brasileiro de Patologia Animal (CBPA), 2004, 43(2023)
Übergeordnetes Werk:	volume:43 ; year:2023

Links:	Link aufrufen Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1590/1678-5150-pvb-7090

Katalog-ID:	DOAJ08089867X

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allfields	10.1590/1678-5150-pvb-7090 doi (DE-627)DOAJ08089867X (DE-599)DOAJ9de799bdbf9f4ad19e813bd7b634b9fb DE-627 ger DE-627 rakwb eng por SF600-1100 Fabio Santiani verfasserin aut Characterization of coccidiosis and evaluation of suggestive cases of subclinical necrotic enteritis in broilers 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ABSTRACT: This study performed the characterization of coccidiosis in broilers and evaluated the occurrence of suggestive cases of necrotic enteritis (NE), seeking if there is an association between the diseases in Brazilian flocks. Two hundred and fifty-six birds from 32 flocks were evaluated. Macroscopic and histopathological lesions were graduated for coccidiosis and NE. Intestinal content was investigated by polymerase chain reaction (PCR) for seven species of Eimeria and by selective anaerobic culture for Clostridium perfringens and identification of the NetB gene. Flocks positive for coccidiosis represented 93.8%. Macroscopic lesions of coccidiosis were Grade 1 for E. acervulina (27%); E. tenella (9.7%) and E. maxima (8.9%). Histopathological evaluation showed Grade 1 in duodenum (38.2%); jejunum (21.4%); cecum (9.3%) and ileum (5%). PCR demonstrated positivity for E. tenella (21.9%), E. maxima (18.8%), and E. acervulina (3.1%). Suggestive macroscopic lesions of necrotic enteritis ranged from Grade 1 (16%), 2 (23%) and 3 (10,9%). Histopathology indicated the absence of necrosis, showing only hemorrhage in the mucosa and submucosa, with the presence of Eimeria spp. Clostridium perfringens type A netB+ was not isolated, demonstrating that macroscopic lesions found mostly in the jejunum did not characterize NE, based on histopathology and negativity of the NetB gene. The study suggests that, due to the high occurrence of coccidiosis, many macroscopic findings suggestive of NE are, in fact, attributed to atypical lesions caused by the reproduction of Eimeria spp. Coccidiosis necrotic enteritis broilers eimeriosis clostridiosis genotyping intestinal disease Veterinary medicine Rodrigo Otávio S. Silva verfasserin aut Carlos Augusto de Oliveira Júnior verfasserin aut Jéssica Aline Withoeft verfasserin aut Thierry G. Cristo verfasserin aut Leonardo S. Costa verfasserin aut Taís Gaspar verfasserin aut Renata A. Casagrande verfasserin aut In Pesquisa Veterinária Brasileira Colégio Brasileiro de Patologia Animal (CBPA), 2004 43(2023) (DE-627)324613520 (DE-600)2028762-8 16785150 nnns volume:43 year:2023 https://doi.org/10.1590/1678-5150-pvb-7090 kostenfrei https://doaj.org/article/9de799bdbf9f4ad19e813bd7b634b9fb kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2023000100205&lng=en&tlng=en kostenfrei http://www.scielo.br/pdf/pvb/v43/1678-5150-pvb-43-e07090.pdf kostenfrei https://doaj.org/toc/1678-5150 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 43 2023
spelling	10.1590/1678-5150-pvb-7090 doi (DE-627)DOAJ08089867X (DE-599)DOAJ9de799bdbf9f4ad19e813bd7b634b9fb DE-627 ger DE-627 rakwb eng por SF600-1100 Fabio Santiani verfasserin aut Characterization of coccidiosis and evaluation of suggestive cases of subclinical necrotic enteritis in broilers 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ABSTRACT: This study performed the characterization of coccidiosis in broilers and evaluated the occurrence of suggestive cases of necrotic enteritis (NE), seeking if there is an association between the diseases in Brazilian flocks. Two hundred and fifty-six birds from 32 flocks were evaluated. Macroscopic and histopathological lesions were graduated for coccidiosis and NE. Intestinal content was investigated by polymerase chain reaction (PCR) for seven species of Eimeria and by selective anaerobic culture for Clostridium perfringens and identification of the NetB gene. Flocks positive for coccidiosis represented 93.8%. Macroscopic lesions of coccidiosis were Grade 1 for E. acervulina (27%); E. tenella (9.7%) and E. maxima (8.9%). Histopathological evaluation showed Grade 1 in duodenum (38.2%); jejunum (21.4%); cecum (9.3%) and ileum (5%). PCR demonstrated positivity for E. tenella (21.9%), E. maxima (18.8%), and E. acervulina (3.1%). Suggestive macroscopic lesions of necrotic enteritis ranged from Grade 1 (16%), 2 (23%) and 3 (10,9%). Histopathology indicated the absence of necrosis, showing only hemorrhage in the mucosa and submucosa, with the presence of Eimeria spp. Clostridium perfringens type A netB+ was not isolated, demonstrating that macroscopic lesions found mostly in the jejunum did not characterize NE, based on histopathology and negativity of the NetB gene. The study suggests that, due to the high occurrence of coccidiosis, many macroscopic findings suggestive of NE are, in fact, attributed to atypical lesions caused by the reproduction of Eimeria spp. Coccidiosis necrotic enteritis broilers eimeriosis clostridiosis genotyping intestinal disease Veterinary medicine Rodrigo Otávio S. Silva verfasserin aut Carlos Augusto de Oliveira Júnior verfasserin aut Jéssica Aline Withoeft verfasserin aut Thierry G. Cristo verfasserin aut Leonardo S. Costa verfasserin aut Taís Gaspar verfasserin aut Renata A. Casagrande verfasserin aut In Pesquisa Veterinária Brasileira Colégio Brasileiro de Patologia Animal (CBPA), 2004 43(2023) (DE-627)324613520 (DE-600)2028762-8 16785150 nnns volume:43 year:2023 https://doi.org/10.1590/1678-5150-pvb-7090 kostenfrei https://doaj.org/article/9de799bdbf9f4ad19e813bd7b634b9fb kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2023000100205&lng=en&tlng=en kostenfrei http://www.scielo.br/pdf/pvb/v43/1678-5150-pvb-43-e07090.pdf kostenfrei https://doaj.org/toc/1678-5150 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 43 2023
allfields_unstemmed	10.1590/1678-5150-pvb-7090 doi (DE-627)DOAJ08089867X (DE-599)DOAJ9de799bdbf9f4ad19e813bd7b634b9fb DE-627 ger DE-627 rakwb eng por SF600-1100 Fabio Santiani verfasserin aut Characterization of coccidiosis and evaluation of suggestive cases of subclinical necrotic enteritis in broilers 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ABSTRACT: This study performed the characterization of coccidiosis in broilers and evaluated the occurrence of suggestive cases of necrotic enteritis (NE), seeking if there is an association between the diseases in Brazilian flocks. Two hundred and fifty-six birds from 32 flocks were evaluated. Macroscopic and histopathological lesions were graduated for coccidiosis and NE. Intestinal content was investigated by polymerase chain reaction (PCR) for seven species of Eimeria and by selective anaerobic culture for Clostridium perfringens and identification of the NetB gene. Flocks positive for coccidiosis represented 93.8%. Macroscopic lesions of coccidiosis were Grade 1 for E. acervulina (27%); E. tenella (9.7%) and E. maxima (8.9%). Histopathological evaluation showed Grade 1 in duodenum (38.2%); jejunum (21.4%); cecum (9.3%) and ileum (5%). PCR demonstrated positivity for E. tenella (21.9%), E. maxima (18.8%), and E. acervulina (3.1%). Suggestive macroscopic lesions of necrotic enteritis ranged from Grade 1 (16%), 2 (23%) and 3 (10,9%). Histopathology indicated the absence of necrosis, showing only hemorrhage in the mucosa and submucosa, with the presence of Eimeria spp. Clostridium perfringens type A netB+ was not isolated, demonstrating that macroscopic lesions found mostly in the jejunum did not characterize NE, based on histopathology and negativity of the NetB gene. The study suggests that, due to the high occurrence of coccidiosis, many macroscopic findings suggestive of NE are, in fact, attributed to atypical lesions caused by the reproduction of Eimeria spp. Coccidiosis necrotic enteritis broilers eimeriosis clostridiosis genotyping intestinal disease Veterinary medicine Rodrigo Otávio S. Silva verfasserin aut Carlos Augusto de Oliveira Júnior verfasserin aut Jéssica Aline Withoeft verfasserin aut Thierry G. Cristo verfasserin aut Leonardo S. Costa verfasserin aut Taís Gaspar verfasserin aut Renata A. Casagrande verfasserin aut In Pesquisa Veterinária Brasileira Colégio Brasileiro de Patologia Animal (CBPA), 2004 43(2023) (DE-627)324613520 (DE-600)2028762-8 16785150 nnns volume:43 year:2023 https://doi.org/10.1590/1678-5150-pvb-7090 kostenfrei https://doaj.org/article/9de799bdbf9f4ad19e813bd7b634b9fb kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2023000100205&lng=en&tlng=en kostenfrei http://www.scielo.br/pdf/pvb/v43/1678-5150-pvb-43-e07090.pdf kostenfrei https://doaj.org/toc/1678-5150 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 43 2023
allfieldsGer	10.1590/1678-5150-pvb-7090 doi (DE-627)DOAJ08089867X (DE-599)DOAJ9de799bdbf9f4ad19e813bd7b634b9fb DE-627 ger DE-627 rakwb eng por SF600-1100 Fabio Santiani verfasserin aut Characterization of coccidiosis and evaluation of suggestive cases of subclinical necrotic enteritis in broilers 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ABSTRACT: This study performed the characterization of coccidiosis in broilers and evaluated the occurrence of suggestive cases of necrotic enteritis (NE), seeking if there is an association between the diseases in Brazilian flocks. Two hundred and fifty-six birds from 32 flocks were evaluated. Macroscopic and histopathological lesions were graduated for coccidiosis and NE. Intestinal content was investigated by polymerase chain reaction (PCR) for seven species of Eimeria and by selective anaerobic culture for Clostridium perfringens and identification of the NetB gene. Flocks positive for coccidiosis represented 93.8%. Macroscopic lesions of coccidiosis were Grade 1 for E. acervulina (27%); E. tenella (9.7%) and E. maxima (8.9%). Histopathological evaluation showed Grade 1 in duodenum (38.2%); jejunum (21.4%); cecum (9.3%) and ileum (5%). PCR demonstrated positivity for E. tenella (21.9%), E. maxima (18.8%), and E. acervulina (3.1%). Suggestive macroscopic lesions of necrotic enteritis ranged from Grade 1 (16%), 2 (23%) and 3 (10,9%). Histopathology indicated the absence of necrosis, showing only hemorrhage in the mucosa and submucosa, with the presence of Eimeria spp. Clostridium perfringens type A netB+ was not isolated, demonstrating that macroscopic lesions found mostly in the jejunum did not characterize NE, based on histopathology and negativity of the NetB gene. The study suggests that, due to the high occurrence of coccidiosis, many macroscopic findings suggestive of NE are, in fact, attributed to atypical lesions caused by the reproduction of Eimeria spp. Coccidiosis necrotic enteritis broilers eimeriosis clostridiosis genotyping intestinal disease Veterinary medicine Rodrigo Otávio S. Silva verfasserin aut Carlos Augusto de Oliveira Júnior verfasserin aut Jéssica Aline Withoeft verfasserin aut Thierry G. Cristo verfasserin aut Leonardo S. Costa verfasserin aut Taís Gaspar verfasserin aut Renata A. Casagrande verfasserin aut In Pesquisa Veterinária Brasileira Colégio Brasileiro de Patologia Animal (CBPA), 2004 43(2023) (DE-627)324613520 (DE-600)2028762-8 16785150 nnns volume:43 year:2023 https://doi.org/10.1590/1678-5150-pvb-7090 kostenfrei https://doaj.org/article/9de799bdbf9f4ad19e813bd7b634b9fb kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2023000100205&lng=en&tlng=en kostenfrei http://www.scielo.br/pdf/pvb/v43/1678-5150-pvb-43-e07090.pdf kostenfrei https://doaj.org/toc/1678-5150 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 43 2023
allfieldsSound	10.1590/1678-5150-pvb-7090 doi (DE-627)DOAJ08089867X (DE-599)DOAJ9de799bdbf9f4ad19e813bd7b634b9fb DE-627 ger DE-627 rakwb eng por SF600-1100 Fabio Santiani verfasserin aut Characterization of coccidiosis and evaluation of suggestive cases of subclinical necrotic enteritis in broilers 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ABSTRACT: This study performed the characterization of coccidiosis in broilers and evaluated the occurrence of suggestive cases of necrotic enteritis (NE), seeking if there is an association between the diseases in Brazilian flocks. Two hundred and fifty-six birds from 32 flocks were evaluated. Macroscopic and histopathological lesions were graduated for coccidiosis and NE. Intestinal content was investigated by polymerase chain reaction (PCR) for seven species of Eimeria and by selective anaerobic culture for Clostridium perfringens and identification of the NetB gene. Flocks positive for coccidiosis represented 93.8%. Macroscopic lesions of coccidiosis were Grade 1 for E. acervulina (27%); E. tenella (9.7%) and E. maxima (8.9%). Histopathological evaluation showed Grade 1 in duodenum (38.2%); jejunum (21.4%); cecum (9.3%) and ileum (5%). PCR demonstrated positivity for E. tenella (21.9%), E. maxima (18.8%), and E. acervulina (3.1%). Suggestive macroscopic lesions of necrotic enteritis ranged from Grade 1 (16%), 2 (23%) and 3 (10,9%). Histopathology indicated the absence of necrosis, showing only hemorrhage in the mucosa and submucosa, with the presence of Eimeria spp. Clostridium perfringens type A netB+ was not isolated, demonstrating that macroscopic lesions found mostly in the jejunum did not characterize NE, based on histopathology and negativity of the NetB gene. The study suggests that, due to the high occurrence of coccidiosis, many macroscopic findings suggestive of NE are, in fact, attributed to atypical lesions caused by the reproduction of Eimeria spp. Coccidiosis necrotic enteritis broilers eimeriosis clostridiosis genotyping intestinal disease Veterinary medicine Rodrigo Otávio S. Silva verfasserin aut Carlos Augusto de Oliveira Júnior verfasserin aut Jéssica Aline Withoeft verfasserin aut Thierry G. Cristo verfasserin aut Leonardo S. Costa verfasserin aut Taís Gaspar verfasserin aut Renata A. Casagrande verfasserin aut In Pesquisa Veterinária Brasileira Colégio Brasileiro de Patologia Animal (CBPA), 2004 43(2023) (DE-627)324613520 (DE-600)2028762-8 16785150 nnns volume:43 year:2023 https://doi.org/10.1590/1678-5150-pvb-7090 kostenfrei https://doaj.org/article/9de799bdbf9f4ad19e813bd7b634b9fb kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2023000100205&lng=en&tlng=en kostenfrei http://www.scielo.br/pdf/pvb/v43/1678-5150-pvb-43-e07090.pdf kostenfrei https://doaj.org/toc/1678-5150 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 43 2023
language	English Portuguese
source	In Pesquisa Veterinária Brasileira 43(2023) volume:43 year:2023
sourceStr	In Pesquisa Veterinária Brasileira 43(2023) volume:43 year:2023
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institution	findex.gbv.de
topic_facet	Coccidiosis necrotic enteritis broilers eimeriosis clostridiosis genotyping intestinal disease Veterinary medicine
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container_title	Pesquisa Veterinária Brasileira
authorswithroles_txt_mv	Fabio Santiani @@aut@@ Rodrigo Otávio S. Silva @@aut@@ Carlos Augusto de Oliveira Júnior @@aut@@ Jéssica Aline Withoeft @@aut@@ Thierry G. Cristo @@aut@@ Leonardo S. Costa @@aut@@ Taís Gaspar @@aut@@ Renata A. Casagrande @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
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callnumber-first	S - Agriculture
author	Fabio Santiani
spellingShingle	Fabio Santiani misc SF600-1100 misc Coccidiosis misc necrotic enteritis misc broilers misc eimeriosis misc clostridiosis misc genotyping misc intestinal disease misc Veterinary medicine Characterization of coccidiosis and evaluation of suggestive cases of subclinical necrotic enteritis in broilers
authorStr	Fabio Santiani
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topic_title	SF600-1100 Characterization of coccidiosis and evaluation of suggestive cases of subclinical necrotic enteritis in broilers Coccidiosis necrotic enteritis broilers eimeriosis clostridiosis genotyping intestinal disease
topic	misc SF600-1100 misc Coccidiosis misc necrotic enteritis misc broilers misc eimeriosis misc clostridiosis misc genotyping misc intestinal disease misc Veterinary medicine
topic_unstemmed	misc SF600-1100 misc Coccidiosis misc necrotic enteritis misc broilers misc eimeriosis misc clostridiosis misc genotyping misc intestinal disease misc Veterinary medicine
topic_browse	misc SF600-1100 misc Coccidiosis misc necrotic enteritis misc broilers misc eimeriosis misc clostridiosis misc genotyping misc intestinal disease misc Veterinary medicine
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title	Characterization of coccidiosis and evaluation of suggestive cases of subclinical necrotic enteritis in broilers
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title_full	Characterization of coccidiosis and evaluation of suggestive cases of subclinical necrotic enteritis in broilers
author_sort	Fabio Santiani
journal	Pesquisa Veterinária Brasileira
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author_browse	Fabio Santiani Rodrigo Otávio S. Silva Carlos Augusto de Oliveira Júnior Jéssica Aline Withoeft Thierry G. Cristo Leonardo S. Costa Taís Gaspar Renata A. Casagrande
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author-letter	Fabio Santiani
doi_str_mv	10.1590/1678-5150-pvb-7090
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title_sort	characterization of coccidiosis and evaluation of suggestive cases of subclinical necrotic enteritis in broilers
callnumber	SF600-1100
title_auth	Characterization of coccidiosis and evaluation of suggestive cases of subclinical necrotic enteritis in broilers
abstract	ABSTRACT: This study performed the characterization of coccidiosis in broilers and evaluated the occurrence of suggestive cases of necrotic enteritis (NE), seeking if there is an association between the diseases in Brazilian flocks. Two hundred and fifty-six birds from 32 flocks were evaluated. Macroscopic and histopathological lesions were graduated for coccidiosis and NE. Intestinal content was investigated by polymerase chain reaction (PCR) for seven species of Eimeria and by selective anaerobic culture for Clostridium perfringens and identification of the NetB gene. Flocks positive for coccidiosis represented 93.8%. Macroscopic lesions of coccidiosis were Grade 1 for E. acervulina (27%); E. tenella (9.7%) and E. maxima (8.9%). Histopathological evaluation showed Grade 1 in duodenum (38.2%); jejunum (21.4%); cecum (9.3%) and ileum (5%). PCR demonstrated positivity for E. tenella (21.9%), E. maxima (18.8%), and E. acervulina (3.1%). Suggestive macroscopic lesions of necrotic enteritis ranged from Grade 1 (16%), 2 (23%) and 3 (10,9%). Histopathology indicated the absence of necrosis, showing only hemorrhage in the mucosa and submucosa, with the presence of Eimeria spp. Clostridium perfringens type A netB+ was not isolated, demonstrating that macroscopic lesions found mostly in the jejunum did not characterize NE, based on histopathology and negativity of the NetB gene. The study suggests that, due to the high occurrence of coccidiosis, many macroscopic findings suggestive of NE are, in fact, attributed to atypical lesions caused by the reproduction of Eimeria spp.
abstractGer	ABSTRACT: This study performed the characterization of coccidiosis in broilers and evaluated the occurrence of suggestive cases of necrotic enteritis (NE), seeking if there is an association between the diseases in Brazilian flocks. Two hundred and fifty-six birds from 32 flocks were evaluated. Macroscopic and histopathological lesions were graduated for coccidiosis and NE. Intestinal content was investigated by polymerase chain reaction (PCR) for seven species of Eimeria and by selective anaerobic culture for Clostridium perfringens and identification of the NetB gene. Flocks positive for coccidiosis represented 93.8%. Macroscopic lesions of coccidiosis were Grade 1 for E. acervulina (27%); E. tenella (9.7%) and E. maxima (8.9%). Histopathological evaluation showed Grade 1 in duodenum (38.2%); jejunum (21.4%); cecum (9.3%) and ileum (5%). PCR demonstrated positivity for E. tenella (21.9%), E. maxima (18.8%), and E. acervulina (3.1%). Suggestive macroscopic lesions of necrotic enteritis ranged from Grade 1 (16%), 2 (23%) and 3 (10,9%). Histopathology indicated the absence of necrosis, showing only hemorrhage in the mucosa and submucosa, with the presence of Eimeria spp. Clostridium perfringens type A netB+ was not isolated, demonstrating that macroscopic lesions found mostly in the jejunum did not characterize NE, based on histopathology and negativity of the NetB gene. The study suggests that, due to the high occurrence of coccidiosis, many macroscopic findings suggestive of NE are, in fact, attributed to atypical lesions caused by the reproduction of Eimeria spp.
abstract_unstemmed	ABSTRACT: This study performed the characterization of coccidiosis in broilers and evaluated the occurrence of suggestive cases of necrotic enteritis (NE), seeking if there is an association between the diseases in Brazilian flocks. Two hundred and fifty-six birds from 32 flocks were evaluated. Macroscopic and histopathological lesions were graduated for coccidiosis and NE. Intestinal content was investigated by polymerase chain reaction (PCR) for seven species of Eimeria and by selective anaerobic culture for Clostridium perfringens and identification of the NetB gene. Flocks positive for coccidiosis represented 93.8%. Macroscopic lesions of coccidiosis were Grade 1 for E. acervulina (27%); E. tenella (9.7%) and E. maxima (8.9%). Histopathological evaluation showed Grade 1 in duodenum (38.2%); jejunum (21.4%); cecum (9.3%) and ileum (5%). PCR demonstrated positivity for E. tenella (21.9%), E. maxima (18.8%), and E. acervulina (3.1%). Suggestive macroscopic lesions of necrotic enteritis ranged from Grade 1 (16%), 2 (23%) and 3 (10,9%). Histopathology indicated the absence of necrosis, showing only hemorrhage in the mucosa and submucosa, with the presence of Eimeria spp. Clostridium perfringens type A netB+ was not isolated, demonstrating that macroscopic lesions found mostly in the jejunum did not characterize NE, based on histopathology and negativity of the NetB gene. The study suggests that, due to the high occurrence of coccidiosis, many macroscopic findings suggestive of NE are, in fact, attributed to atypical lesions caused by the reproduction of Eimeria spp.
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title_short	Characterization of coccidiosis and evaluation of suggestive cases of subclinical necrotic enteritis in broilers
url	https://doi.org/10.1590/1678-5150-pvb-7090 https://doaj.org/article/9de799bdbf9f4ad19e813bd7b634b9fb http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2023000100205&lng=en&tlng=en http://www.scielo.br/pdf/pvb/v43/1678-5150-pvb-43-e07090.pdf https://doaj.org/toc/1678-5150
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author2	Rodrigo Otávio S. Silva Carlos Augusto de Oliveira Júnior Jéssica Aline Withoeft Thierry G. Cristo Leonardo S. Costa Taís Gaspar Renata A. Casagrande
author2Str	Rodrigo Otávio S. Silva Carlos Augusto de Oliveira Júnior Jéssica Aline Withoeft Thierry G. Cristo Leonardo S. Costa Taís Gaspar Renata A. Casagrande
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up_date	2024-07-03T17:08:48.680Z
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Characterization of coccidiosis and evaluation of suggestive cases of subclinical necrotic enteritis in broilers

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