Discovering a Dihydrofluorescein Analogue as a Promising Fluorescence Substrate to HRP

Horseradish peroxidase (HRP) combined with its fluorescence substrates is attracting increasing attention for biochemical analysis. Amplex red is the most widely used fluorescence substrate to HRP; however, it suffers from some drawbacks, such as nonspecific responsiveness toward carboxylesterases....
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Autor*in:	Jiayan Zhu [verfasserIn] Ting Li [verfasserIn] Shihui Zhang [verfasserIn] Xiaomei Zou [verfasserIn] Yingchun Zhou [verfasserIn] Weiguo Lu [verfasserIn] Zhihui Liu [verfasserIn] Tao Deng [verfasserIn] Fang Liu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	horseradish peroxidase (HRP) fluorescence substrate to HRP dichlorodihydrofluorescein (DCFH) ELISA fluorescence immunoassay

Übergeordnetes Werk:	In: Chemosensors - MDPI AG, 2013, 11(2023), 2, p 152
Übergeordnetes Werk:	volume:11 ; year:2023 ; number:2, p 152

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/chemosensors11020152

Katalog-ID:	DOAJ080983855

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520			\|a Horseradish peroxidase (HRP) combined with its fluorescence substrates is attracting increasing attention for biochemical analysis. Amplex red is the most widely used fluorescence substrate to HRP; however, it suffers from some drawbacks, such as nonspecific responsiveness toward carboxylesterases. Discovering a new small molecular fluorescence substrate with improved sensitivity and selectivity for HRP is thus desired. Herein, three dihydrofluorescein derivatives (DCFHs) are presented to serve as HRP substrates through fluorescence turn-on methods. The most promising one, 2,7-dichloro-9-(2-(hydroxymethyl)phenyl)-9H-xanthene-3,6-diol (<b<DCFH-1</b<), exhibited excellent sensitivity in the detection of HRP. Moreover, <b<DCFH-1</b< does not respond to carboxylesterase, thus holding advantages over Amplex red. In the further study, the detection reagent in the commercial ELISA kits was replaced with <b<DCFH-1</b< to establish a new fluorescence ELISA, which works very well in the quantification of inflammatory cytokine biomarkers from in vitro models.
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spelling	10.3390/chemosensors11020152 doi (DE-627)DOAJ080983855 (DE-599)DOAJfb572347469f4ba198e656f99b084068 DE-627 ger DE-627 rakwb eng QD415-436 Jiayan Zhu verfasserin aut Discovering a Dihydrofluorescein Analogue as a Promising Fluorescence Substrate to HRP 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Horseradish peroxidase (HRP) combined with its fluorescence substrates is attracting increasing attention for biochemical analysis. Amplex red is the most widely used fluorescence substrate to HRP; however, it suffers from some drawbacks, such as nonspecific responsiveness toward carboxylesterases. Discovering a new small molecular fluorescence substrate with improved sensitivity and selectivity for HRP is thus desired. Herein, three dihydrofluorescein derivatives (DCFHs) are presented to serve as HRP substrates through fluorescence turn-on methods. The most promising one, 2,7-dichloro-9-(2-(hydroxymethyl)phenyl)-9H-xanthene-3,6-diol (<b<DCFH-1</b<), exhibited excellent sensitivity in the detection of HRP. Moreover, <b<DCFH-1</b< does not respond to carboxylesterase, thus holding advantages over Amplex red. In the further study, the detection reagent in the commercial ELISA kits was replaced with <b<DCFH-1</b< to establish a new fluorescence ELISA, which works very well in the quantification of inflammatory cytokine biomarkers from in vitro models. horseradish peroxidase (HRP) fluorescence substrate to HRP dichlorodihydrofluorescein (DCFH) ELISA fluorescence immunoassay Biochemistry Ting Li verfasserin aut Shihui Zhang verfasserin aut Xiaomei Zou verfasserin aut Yingchun Zhou verfasserin aut Weiguo Lu verfasserin aut Zhihui Liu verfasserin aut Tao Deng verfasserin aut Fang Liu verfasserin aut In Chemosensors MDPI AG, 2013 11(2023), 2, p 152 (DE-627)737287594 (DE-600)2704218-2 22279040 nnns volume:11 year:2023 number:2, p 152 https://doi.org/10.3390/chemosensors11020152 kostenfrei https://doaj.org/article/fb572347469f4ba198e656f99b084068 kostenfrei https://www.mdpi.com/2227-9040/11/2/152 kostenfrei https://doaj.org/toc/2227-9040 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2023 2, p 152
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allfieldsSound	10.3390/chemosensors11020152 doi (DE-627)DOAJ080983855 (DE-599)DOAJfb572347469f4ba198e656f99b084068 DE-627 ger DE-627 rakwb eng QD415-436 Jiayan Zhu verfasserin aut Discovering a Dihydrofluorescein Analogue as a Promising Fluorescence Substrate to HRP 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Horseradish peroxidase (HRP) combined with its fluorescence substrates is attracting increasing attention for biochemical analysis. Amplex red is the most widely used fluorescence substrate to HRP; however, it suffers from some drawbacks, such as nonspecific responsiveness toward carboxylesterases. Discovering a new small molecular fluorescence substrate with improved sensitivity and selectivity for HRP is thus desired. Herein, three dihydrofluorescein derivatives (DCFHs) are presented to serve as HRP substrates through fluorescence turn-on methods. The most promising one, 2,7-dichloro-9-(2-(hydroxymethyl)phenyl)-9H-xanthene-3,6-diol (<b<DCFH-1</b<), exhibited excellent sensitivity in the detection of HRP. Moreover, <b<DCFH-1</b< does not respond to carboxylesterase, thus holding advantages over Amplex red. In the further study, the detection reagent in the commercial ELISA kits was replaced with <b<DCFH-1</b< to establish a new fluorescence ELISA, which works very well in the quantification of inflammatory cytokine biomarkers from in vitro models. horseradish peroxidase (HRP) fluorescence substrate to HRP dichlorodihydrofluorescein (DCFH) ELISA fluorescence immunoassay Biochemistry Ting Li verfasserin aut Shihui Zhang verfasserin aut Xiaomei Zou verfasserin aut Yingchun Zhou verfasserin aut Weiguo Lu verfasserin aut Zhihui Liu verfasserin aut Tao Deng verfasserin aut Fang Liu verfasserin aut In Chemosensors MDPI AG, 2013 11(2023), 2, p 152 (DE-627)737287594 (DE-600)2704218-2 22279040 nnns volume:11 year:2023 number:2, p 152 https://doi.org/10.3390/chemosensors11020152 kostenfrei https://doaj.org/article/fb572347469f4ba198e656f99b084068 kostenfrei https://www.mdpi.com/2227-9040/11/2/152 kostenfrei https://doaj.org/toc/2227-9040 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2023 2, p 152
language	English
source	In Chemosensors 11(2023), 2, p 152 volume:11 year:2023 number:2, p 152
sourceStr	In Chemosensors 11(2023), 2, p 152 volume:11 year:2023 number:2, p 152
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	horseradish peroxidase (HRP) fluorescence substrate to HRP dichlorodihydrofluorescein (DCFH) ELISA fluorescence immunoassay Biochemistry
isfreeaccess_bool	true
container_title	Chemosensors
authorswithroles_txt_mv	Jiayan Zhu @@aut@@ Ting Li @@aut@@ Shihui Zhang @@aut@@ Xiaomei Zou @@aut@@ Yingchun Zhou @@aut@@ Weiguo Lu @@aut@@ Zhihui Liu @@aut@@ Tao Deng @@aut@@ Fang Liu @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
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callnumber-first	Q - Science
author	Jiayan Zhu
spellingShingle	Jiayan Zhu misc QD415-436 misc horseradish peroxidase (HRP) misc fluorescence substrate to HRP misc dichlorodihydrofluorescein (DCFH) misc ELISA misc fluorescence immunoassay misc Biochemistry Discovering a Dihydrofluorescein Analogue as a Promising Fluorescence Substrate to HRP
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topic_title	QD415-436 Discovering a Dihydrofluorescein Analogue as a Promising Fluorescence Substrate to HRP horseradish peroxidase (HRP) fluorescence substrate to HRP dichlorodihydrofluorescein (DCFH) ELISA fluorescence immunoassay
topic	misc QD415-436 misc horseradish peroxidase (HRP) misc fluorescence substrate to HRP misc dichlorodihydrofluorescein (DCFH) misc ELISA misc fluorescence immunoassay misc Biochemistry
topic_unstemmed	misc QD415-436 misc horseradish peroxidase (HRP) misc fluorescence substrate to HRP misc dichlorodihydrofluorescein (DCFH) misc ELISA misc fluorescence immunoassay misc Biochemistry
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title	Discovering a Dihydrofluorescein Analogue as a Promising Fluorescence Substrate to HRP
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title_full	Discovering a Dihydrofluorescein Analogue as a Promising Fluorescence Substrate to HRP
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author_browse	Jiayan Zhu Ting Li Shihui Zhang Xiaomei Zou Yingchun Zhou Weiguo Lu Zhihui Liu Tao Deng Fang Liu
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title_sort	discovering a dihydrofluorescein analogue as a promising fluorescence substrate to hrp
callnumber	QD415-436
title_auth	Discovering a Dihydrofluorescein Analogue as a Promising Fluorescence Substrate to HRP
abstract	Horseradish peroxidase (HRP) combined with its fluorescence substrates is attracting increasing attention for biochemical analysis. Amplex red is the most widely used fluorescence substrate to HRP; however, it suffers from some drawbacks, such as nonspecific responsiveness toward carboxylesterases. Discovering a new small molecular fluorescence substrate with improved sensitivity and selectivity for HRP is thus desired. Herein, three dihydrofluorescein derivatives (DCFHs) are presented to serve as HRP substrates through fluorescence turn-on methods. The most promising one, 2,7-dichloro-9-(2-(hydroxymethyl)phenyl)-9H-xanthene-3,6-diol (<b<DCFH-1</b<), exhibited excellent sensitivity in the detection of HRP. Moreover, <b<DCFH-1</b< does not respond to carboxylesterase, thus holding advantages over Amplex red. In the further study, the detection reagent in the commercial ELISA kits was replaced with <b<DCFH-1</b< to establish a new fluorescence ELISA, which works very well in the quantification of inflammatory cytokine biomarkers from in vitro models.
abstractGer	Horseradish peroxidase (HRP) combined with its fluorescence substrates is attracting increasing attention for biochemical analysis. Amplex red is the most widely used fluorescence substrate to HRP; however, it suffers from some drawbacks, such as nonspecific responsiveness toward carboxylesterases. Discovering a new small molecular fluorescence substrate with improved sensitivity and selectivity for HRP is thus desired. Herein, three dihydrofluorescein derivatives (DCFHs) are presented to serve as HRP substrates through fluorescence turn-on methods. The most promising one, 2,7-dichloro-9-(2-(hydroxymethyl)phenyl)-9H-xanthene-3,6-diol (<b<DCFH-1</b<), exhibited excellent sensitivity in the detection of HRP. Moreover, <b<DCFH-1</b< does not respond to carboxylesterase, thus holding advantages over Amplex red. In the further study, the detection reagent in the commercial ELISA kits was replaced with <b<DCFH-1</b< to establish a new fluorescence ELISA, which works very well in the quantification of inflammatory cytokine biomarkers from in vitro models.
abstract_unstemmed	Horseradish peroxidase (HRP) combined with its fluorescence substrates is attracting increasing attention for biochemical analysis. Amplex red is the most widely used fluorescence substrate to HRP; however, it suffers from some drawbacks, such as nonspecific responsiveness toward carboxylesterases. Discovering a new small molecular fluorescence substrate with improved sensitivity and selectivity for HRP is thus desired. Herein, three dihydrofluorescein derivatives (DCFHs) are presented to serve as HRP substrates through fluorescence turn-on methods. The most promising one, 2,7-dichloro-9-(2-(hydroxymethyl)phenyl)-9H-xanthene-3,6-diol (<b<DCFH-1</b<), exhibited excellent sensitivity in the detection of HRP. Moreover, <b<DCFH-1</b< does not respond to carboxylesterase, thus holding advantages over Amplex red. In the further study, the detection reagent in the commercial ELISA kits was replaced with <b<DCFH-1</b< to establish a new fluorescence ELISA, which works very well in the quantification of inflammatory cytokine biomarkers from in vitro models.
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title_short	Discovering a Dihydrofluorescein Analogue as a Promising Fluorescence Substrate to HRP
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