Small extracellular vesicles released from germinated kiwi pollen (pollensomes) present characteristics similar to mammalian exosomes and carry a plant homolog of ALIX

IntroductionIn the last decade, it has been discovered that allergen-bearing extracellular nanovesicles, termed “pollensomes”, are released by pollen during germination. These extracellular vesicles (EVs) may play an important role in pollen-pistil interaction during fertilization, stabilizing the s...
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Autor*in:	Chiara Suanno [verfasserIn] Elisa Tonoli [verfasserIn] Enzo Fornari [verfasserIn] Maria P. Savoca [verfasserIn] Iris Aloisi [verfasserIn] Luigi Parrotta [verfasserIn] Claudia Faleri [verfasserIn] Giampiero Cai [verfasserIn] Clare Coveney [verfasserIn] David J. Boocock [verfasserIn] Elisabetta A. M. Verderio [verfasserIn] Stefano Del Duca [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	pollensomes nanovesicles pollen tube pollination exosomes signaling

Übergeordnetes Werk:	In: Frontiers in Plant Science - Frontiers Media S.A., 2011, 14(2023)
Übergeordnetes Werk:	volume:14 ; year:2023

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fpls.2023.1090026

Katalog-ID:	DOAJ081539967

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520			\|a IntroductionIn the last decade, it has been discovered that allergen-bearing extracellular nanovesicles, termed “pollensomes”, are released by pollen during germination. These extracellular vesicles (EVs) may play an important role in pollen-pistil interaction during fertilization, stabilizing the secreted bioactive molecules and allowing long-distance signaling. However, the molecular composition and the biological role of these EVs are still unclear. The present study had two main aims: (I) to clarify whether pollen germination is needed to release pollensomes, or if they can be secreted also in high humidity conditions; and (II) to investigate the molecular features of pollensomes following the most recent guidelines for EVs isolation and identification.MethodsTo do so, pollensomes were isolated from hydrated and germinated kiwi (Actinidia chinensis Planch.) pollen, and characterized using imaging techniques, immunoblotting, and proteomics.ResultsThese analyses revealed that only germinated kiwi pollen released detectable concentrations of nanoparticles compatible with small EVs for shape and protein content. Moreover, a plant homolog of ALIX, which is a well-recognized and accepted marker of small EVs and exosomes in mammals, was found in pollensomes.DiscussionThe presence of this protein, along with other proteins involved in endocytosis, is consistent with the hypothesis that pollensomes could comprehend a prominent subpopulation of plant exosome-like vesicles.
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allfields	10.3389/fpls.2023.1090026 doi (DE-627)DOAJ081539967 (DE-599)DOAJ71e3d1997a0a435ab8a200e123d6d12d DE-627 ger DE-627 rakwb eng SB1-1110 Chiara Suanno verfasserin aut Small extracellular vesicles released from germinated kiwi pollen (pollensomes) present characteristics similar to mammalian exosomes and carry a plant homolog of ALIX 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier IntroductionIn the last decade, it has been discovered that allergen-bearing extracellular nanovesicles, termed “pollensomes”, are released by pollen during germination. These extracellular vesicles (EVs) may play an important role in pollen-pistil interaction during fertilization, stabilizing the secreted bioactive molecules and allowing long-distance signaling. However, the molecular composition and the biological role of these EVs are still unclear. The present study had two main aims: (I) to clarify whether pollen germination is needed to release pollensomes, or if they can be secreted also in high humidity conditions; and (II) to investigate the molecular features of pollensomes following the most recent guidelines for EVs isolation and identification.MethodsTo do so, pollensomes were isolated from hydrated and germinated kiwi (Actinidia chinensis Planch.) pollen, and characterized using imaging techniques, immunoblotting, and proteomics.ResultsThese analyses revealed that only germinated kiwi pollen released detectable concentrations of nanoparticles compatible with small EVs for shape and protein content. Moreover, a plant homolog of ALIX, which is a well-recognized and accepted marker of small EVs and exosomes in mammals, was found in pollensomes.DiscussionThe presence of this protein, along with other proteins involved in endocytosis, is consistent with the hypothesis that pollensomes could comprehend a prominent subpopulation of plant exosome-like vesicles. pollensomes nanovesicles pollen tube pollination exosomes signaling Plant culture Elisa Tonoli verfasserin aut Enzo Fornari verfasserin aut Maria P. Savoca verfasserin aut Iris Aloisi verfasserin aut Luigi Parrotta verfasserin aut Luigi Parrotta verfasserin aut Claudia Faleri verfasserin aut Giampiero Cai verfasserin aut Clare Coveney verfasserin aut Clare Coveney verfasserin aut David J. Boocock verfasserin aut David J. Boocock verfasserin aut Elisabetta A. M. Verderio verfasserin aut Elisabetta A. M. Verderio verfasserin aut Stefano Del Duca verfasserin aut Stefano Del Duca verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 14(2023) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:14 year:2023 https://doi.org/10.3389/fpls.2023.1090026 kostenfrei https://doaj.org/article/71e3d1997a0a435ab8a200e123d6d12d kostenfrei https://www.frontiersin.org/articles/10.3389/fpls.2023.1090026/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023
spelling	10.3389/fpls.2023.1090026 doi (DE-627)DOAJ081539967 (DE-599)DOAJ71e3d1997a0a435ab8a200e123d6d12d DE-627 ger DE-627 rakwb eng SB1-1110 Chiara Suanno verfasserin aut Small extracellular vesicles released from germinated kiwi pollen (pollensomes) present characteristics similar to mammalian exosomes and carry a plant homolog of ALIX 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier IntroductionIn the last decade, it has been discovered that allergen-bearing extracellular nanovesicles, termed “pollensomes”, are released by pollen during germination. These extracellular vesicles (EVs) may play an important role in pollen-pistil interaction during fertilization, stabilizing the secreted bioactive molecules and allowing long-distance signaling. However, the molecular composition and the biological role of these EVs are still unclear. The present study had two main aims: (I) to clarify whether pollen germination is needed to release pollensomes, or if they can be secreted also in high humidity conditions; and (II) to investigate the molecular features of pollensomes following the most recent guidelines for EVs isolation and identification.MethodsTo do so, pollensomes were isolated from hydrated and germinated kiwi (Actinidia chinensis Planch.) pollen, and characterized using imaging techniques, immunoblotting, and proteomics.ResultsThese analyses revealed that only germinated kiwi pollen released detectable concentrations of nanoparticles compatible with small EVs for shape and protein content. Moreover, a plant homolog of ALIX, which is a well-recognized and accepted marker of small EVs and exosomes in mammals, was found in pollensomes.DiscussionThe presence of this protein, along with other proteins involved in endocytosis, is consistent with the hypothesis that pollensomes could comprehend a prominent subpopulation of plant exosome-like vesicles. pollensomes nanovesicles pollen tube pollination exosomes signaling Plant culture Elisa Tonoli verfasserin aut Enzo Fornari verfasserin aut Maria P. Savoca verfasserin aut Iris Aloisi verfasserin aut Luigi Parrotta verfasserin aut Luigi Parrotta verfasserin aut Claudia Faleri verfasserin aut Giampiero Cai verfasserin aut Clare Coveney verfasserin aut Clare Coveney verfasserin aut David J. Boocock verfasserin aut David J. Boocock verfasserin aut Elisabetta A. M. Verderio verfasserin aut Elisabetta A. M. Verderio verfasserin aut Stefano Del Duca verfasserin aut Stefano Del Duca verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 14(2023) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:14 year:2023 https://doi.org/10.3389/fpls.2023.1090026 kostenfrei https://doaj.org/article/71e3d1997a0a435ab8a200e123d6d12d kostenfrei https://www.frontiersin.org/articles/10.3389/fpls.2023.1090026/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023
allfields_unstemmed	10.3389/fpls.2023.1090026 doi (DE-627)DOAJ081539967 (DE-599)DOAJ71e3d1997a0a435ab8a200e123d6d12d DE-627 ger DE-627 rakwb eng SB1-1110 Chiara Suanno verfasserin aut Small extracellular vesicles released from germinated kiwi pollen (pollensomes) present characteristics similar to mammalian exosomes and carry a plant homolog of ALIX 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier IntroductionIn the last decade, it has been discovered that allergen-bearing extracellular nanovesicles, termed “pollensomes”, are released by pollen during germination. These extracellular vesicles (EVs) may play an important role in pollen-pistil interaction during fertilization, stabilizing the secreted bioactive molecules and allowing long-distance signaling. However, the molecular composition and the biological role of these EVs are still unclear. The present study had two main aims: (I) to clarify whether pollen germination is needed to release pollensomes, or if they can be secreted also in high humidity conditions; and (II) to investigate the molecular features of pollensomes following the most recent guidelines for EVs isolation and identification.MethodsTo do so, pollensomes were isolated from hydrated and germinated kiwi (Actinidia chinensis Planch.) pollen, and characterized using imaging techniques, immunoblotting, and proteomics.ResultsThese analyses revealed that only germinated kiwi pollen released detectable concentrations of nanoparticles compatible with small EVs for shape and protein content. Moreover, a plant homolog of ALIX, which is a well-recognized and accepted marker of small EVs and exosomes in mammals, was found in pollensomes.DiscussionThe presence of this protein, along with other proteins involved in endocytosis, is consistent with the hypothesis that pollensomes could comprehend a prominent subpopulation of plant exosome-like vesicles. pollensomes nanovesicles pollen tube pollination exosomes signaling Plant culture Elisa Tonoli verfasserin aut Enzo Fornari verfasserin aut Maria P. Savoca verfasserin aut Iris Aloisi verfasserin aut Luigi Parrotta verfasserin aut Luigi Parrotta verfasserin aut Claudia Faleri verfasserin aut Giampiero Cai verfasserin aut Clare Coveney verfasserin aut Clare Coveney verfasserin aut David J. Boocock verfasserin aut David J. Boocock verfasserin aut Elisabetta A. M. Verderio verfasserin aut Elisabetta A. M. Verderio verfasserin aut Stefano Del Duca verfasserin aut Stefano Del Duca verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 14(2023) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:14 year:2023 https://doi.org/10.3389/fpls.2023.1090026 kostenfrei https://doaj.org/article/71e3d1997a0a435ab8a200e123d6d12d kostenfrei https://www.frontiersin.org/articles/10.3389/fpls.2023.1090026/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023
allfieldsGer	10.3389/fpls.2023.1090026 doi (DE-627)DOAJ081539967 (DE-599)DOAJ71e3d1997a0a435ab8a200e123d6d12d DE-627 ger DE-627 rakwb eng SB1-1110 Chiara Suanno verfasserin aut Small extracellular vesicles released from germinated kiwi pollen (pollensomes) present characteristics similar to mammalian exosomes and carry a plant homolog of ALIX 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier IntroductionIn the last decade, it has been discovered that allergen-bearing extracellular nanovesicles, termed “pollensomes”, are released by pollen during germination. These extracellular vesicles (EVs) may play an important role in pollen-pistil interaction during fertilization, stabilizing the secreted bioactive molecules and allowing long-distance signaling. However, the molecular composition and the biological role of these EVs are still unclear. The present study had two main aims: (I) to clarify whether pollen germination is needed to release pollensomes, or if they can be secreted also in high humidity conditions; and (II) to investigate the molecular features of pollensomes following the most recent guidelines for EVs isolation and identification.MethodsTo do so, pollensomes were isolated from hydrated and germinated kiwi (Actinidia chinensis Planch.) pollen, and characterized using imaging techniques, immunoblotting, and proteomics.ResultsThese analyses revealed that only germinated kiwi pollen released detectable concentrations of nanoparticles compatible with small EVs for shape and protein content. Moreover, a plant homolog of ALIX, which is a well-recognized and accepted marker of small EVs and exosomes in mammals, was found in pollensomes.DiscussionThe presence of this protein, along with other proteins involved in endocytosis, is consistent with the hypothesis that pollensomes could comprehend a prominent subpopulation of plant exosome-like vesicles. pollensomes nanovesicles pollen tube pollination exosomes signaling Plant culture Elisa Tonoli verfasserin aut Enzo Fornari verfasserin aut Maria P. Savoca verfasserin aut Iris Aloisi verfasserin aut Luigi Parrotta verfasserin aut Luigi Parrotta verfasserin aut Claudia Faleri verfasserin aut Giampiero Cai verfasserin aut Clare Coveney verfasserin aut Clare Coveney verfasserin aut David J. Boocock verfasserin aut David J. Boocock verfasserin aut Elisabetta A. M. Verderio verfasserin aut Elisabetta A. M. Verderio verfasserin aut Stefano Del Duca verfasserin aut Stefano Del Duca verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 14(2023) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:14 year:2023 https://doi.org/10.3389/fpls.2023.1090026 kostenfrei https://doaj.org/article/71e3d1997a0a435ab8a200e123d6d12d kostenfrei https://www.frontiersin.org/articles/10.3389/fpls.2023.1090026/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023
allfieldsSound	10.3389/fpls.2023.1090026 doi (DE-627)DOAJ081539967 (DE-599)DOAJ71e3d1997a0a435ab8a200e123d6d12d DE-627 ger DE-627 rakwb eng SB1-1110 Chiara Suanno verfasserin aut Small extracellular vesicles released from germinated kiwi pollen (pollensomes) present characteristics similar to mammalian exosomes and carry a plant homolog of ALIX 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier IntroductionIn the last decade, it has been discovered that allergen-bearing extracellular nanovesicles, termed “pollensomes”, are released by pollen during germination. These extracellular vesicles (EVs) may play an important role in pollen-pistil interaction during fertilization, stabilizing the secreted bioactive molecules and allowing long-distance signaling. However, the molecular composition and the biological role of these EVs are still unclear. The present study had two main aims: (I) to clarify whether pollen germination is needed to release pollensomes, or if they can be secreted also in high humidity conditions; and (II) to investigate the molecular features of pollensomes following the most recent guidelines for EVs isolation and identification.MethodsTo do so, pollensomes were isolated from hydrated and germinated kiwi (Actinidia chinensis Planch.) pollen, and characterized using imaging techniques, immunoblotting, and proteomics.ResultsThese analyses revealed that only germinated kiwi pollen released detectable concentrations of nanoparticles compatible with small EVs for shape and protein content. Moreover, a plant homolog of ALIX, which is a well-recognized and accepted marker of small EVs and exosomes in mammals, was found in pollensomes.DiscussionThe presence of this protein, along with other proteins involved in endocytosis, is consistent with the hypothesis that pollensomes could comprehend a prominent subpopulation of plant exosome-like vesicles. pollensomes nanovesicles pollen tube pollination exosomes signaling Plant culture Elisa Tonoli verfasserin aut Enzo Fornari verfasserin aut Maria P. Savoca verfasserin aut Iris Aloisi verfasserin aut Luigi Parrotta verfasserin aut Luigi Parrotta verfasserin aut Claudia Faleri verfasserin aut Giampiero Cai verfasserin aut Clare Coveney verfasserin aut Clare Coveney verfasserin aut David J. Boocock verfasserin aut David J. Boocock verfasserin aut Elisabetta A. M. Verderio verfasserin aut Elisabetta A. M. Verderio verfasserin aut Stefano Del Duca verfasserin aut Stefano Del Duca verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 14(2023) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:14 year:2023 https://doi.org/10.3389/fpls.2023.1090026 kostenfrei https://doaj.org/article/71e3d1997a0a435ab8a200e123d6d12d kostenfrei https://www.frontiersin.org/articles/10.3389/fpls.2023.1090026/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023
language	English
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authorswithroles_txt_mv	Chiara Suanno @@aut@@ Elisa Tonoli @@aut@@ Enzo Fornari @@aut@@ Maria P. Savoca @@aut@@ Iris Aloisi @@aut@@ Luigi Parrotta @@aut@@ Claudia Faleri @@aut@@ Giampiero Cai @@aut@@ Clare Coveney @@aut@@ David J. Boocock @@aut@@ Elisabetta A. M. Verderio @@aut@@ Stefano Del Duca @@aut@@
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callnumber-first	S - Agriculture
author	Chiara Suanno
spellingShingle	Chiara Suanno misc SB1-1110 misc pollensomes misc nanovesicles misc pollen tube misc pollination misc exosomes misc signaling misc Plant culture Small extracellular vesicles released from germinated kiwi pollen (pollensomes) present characteristics similar to mammalian exosomes and carry a plant homolog of ALIX
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topic_title	SB1-1110 Small extracellular vesicles released from germinated kiwi pollen (pollensomes) present characteristics similar to mammalian exosomes and carry a plant homolog of ALIX pollensomes nanovesicles pollen tube pollination exosomes signaling
topic	misc SB1-1110 misc pollensomes misc nanovesicles misc pollen tube misc pollination misc exosomes misc signaling misc Plant culture
topic_unstemmed	misc SB1-1110 misc pollensomes misc nanovesicles misc pollen tube misc pollination misc exosomes misc signaling misc Plant culture
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title	Small extracellular vesicles released from germinated kiwi pollen (pollensomes) present characteristics similar to mammalian exosomes and carry a plant homolog of ALIX
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author_browse	Chiara Suanno Elisa Tonoli Enzo Fornari Maria P. Savoca Iris Aloisi Luigi Parrotta Claudia Faleri Giampiero Cai Clare Coveney David J. Boocock Elisabetta A. M. Verderio Stefano Del Duca
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title_sort	small extracellular vesicles released from germinated kiwi pollen (pollensomes) present characteristics similar to mammalian exosomes and carry a plant homolog of alix
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title_auth	Small extracellular vesicles released from germinated kiwi pollen (pollensomes) present characteristics similar to mammalian exosomes and carry a plant homolog of ALIX
abstract	IntroductionIn the last decade, it has been discovered that allergen-bearing extracellular nanovesicles, termed “pollensomes”, are released by pollen during germination. These extracellular vesicles (EVs) may play an important role in pollen-pistil interaction during fertilization, stabilizing the secreted bioactive molecules and allowing long-distance signaling. However, the molecular composition and the biological role of these EVs are still unclear. The present study had two main aims: (I) to clarify whether pollen germination is needed to release pollensomes, or if they can be secreted also in high humidity conditions; and (II) to investigate the molecular features of pollensomes following the most recent guidelines for EVs isolation and identification.MethodsTo do so, pollensomes were isolated from hydrated and germinated kiwi (Actinidia chinensis Planch.) pollen, and characterized using imaging techniques, immunoblotting, and proteomics.ResultsThese analyses revealed that only germinated kiwi pollen released detectable concentrations of nanoparticles compatible with small EVs for shape and protein content. Moreover, a plant homolog of ALIX, which is a well-recognized and accepted marker of small EVs and exosomes in mammals, was found in pollensomes.DiscussionThe presence of this protein, along with other proteins involved in endocytosis, is consistent with the hypothesis that pollensomes could comprehend a prominent subpopulation of plant exosome-like vesicles.
abstractGer	IntroductionIn the last decade, it has been discovered that allergen-bearing extracellular nanovesicles, termed “pollensomes”, are released by pollen during germination. These extracellular vesicles (EVs) may play an important role in pollen-pistil interaction during fertilization, stabilizing the secreted bioactive molecules and allowing long-distance signaling. However, the molecular composition and the biological role of these EVs are still unclear. The present study had two main aims: (I) to clarify whether pollen germination is needed to release pollensomes, or if they can be secreted also in high humidity conditions; and (II) to investigate the molecular features of pollensomes following the most recent guidelines for EVs isolation and identification.MethodsTo do so, pollensomes were isolated from hydrated and germinated kiwi (Actinidia chinensis Planch.) pollen, and characterized using imaging techniques, immunoblotting, and proteomics.ResultsThese analyses revealed that only germinated kiwi pollen released detectable concentrations of nanoparticles compatible with small EVs for shape and protein content. Moreover, a plant homolog of ALIX, which is a well-recognized and accepted marker of small EVs and exosomes in mammals, was found in pollensomes.DiscussionThe presence of this protein, along with other proteins involved in endocytosis, is consistent with the hypothesis that pollensomes could comprehend a prominent subpopulation of plant exosome-like vesicles.
abstract_unstemmed	IntroductionIn the last decade, it has been discovered that allergen-bearing extracellular nanovesicles, termed “pollensomes”, are released by pollen during germination. These extracellular vesicles (EVs) may play an important role in pollen-pistil interaction during fertilization, stabilizing the secreted bioactive molecules and allowing long-distance signaling. However, the molecular composition and the biological role of these EVs are still unclear. The present study had two main aims: (I) to clarify whether pollen germination is needed to release pollensomes, or if they can be secreted also in high humidity conditions; and (II) to investigate the molecular features of pollensomes following the most recent guidelines for EVs isolation and identification.MethodsTo do so, pollensomes were isolated from hydrated and germinated kiwi (Actinidia chinensis Planch.) pollen, and characterized using imaging techniques, immunoblotting, and proteomics.ResultsThese analyses revealed that only germinated kiwi pollen released detectable concentrations of nanoparticles compatible with small EVs for shape and protein content. Moreover, a plant homolog of ALIX, which is a well-recognized and accepted marker of small EVs and exosomes in mammals, was found in pollensomes.DiscussionThe presence of this protein, along with other proteins involved in endocytosis, is consistent with the hypothesis that pollensomes could comprehend a prominent subpopulation of plant exosome-like vesicles.
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title_short	Small extracellular vesicles released from germinated kiwi pollen (pollensomes) present characteristics similar to mammalian exosomes and carry a plant homolog of ALIX
url	https://doi.org/10.3389/fpls.2023.1090026 https://doaj.org/article/71e3d1997a0a435ab8a200e123d6d12d https://www.frontiersin.org/articles/10.3389/fpls.2023.1090026/full https://doaj.org/toc/1664-462X
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author2	Elisa Tonoli Enzo Fornari Maria P. Savoca Iris Aloisi Luigi Parrotta Claudia Faleri Giampiero Cai Clare Coveney David J. Boocock Elisabetta A. M. Verderio Stefano Del Duca
author2Str	Elisa Tonoli Enzo Fornari Maria P. Savoca Iris Aloisi Luigi Parrotta Claudia Faleri Giampiero Cai Clare Coveney David J. Boocock Elisabetta A. M. Verderio Stefano Del Duca
ppnlink	662359240
callnumber-subject	SB - Plant Culture
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isOA_txt	true
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doi_str	10.3389/fpls.2023.1090026
callnumber-a	SB1-1110
up_date	2024-07-03T20:30:39.269Z
_version_	1803591236212228096
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These extracellular vesicles (EVs) may play an important role in pollen-pistil interaction during fertilization, stabilizing the secreted bioactive molecules and allowing long-distance signaling. However, the molecular composition and the biological role of these EVs are still unclear. The present study had two main aims: (I) to clarify whether pollen germination is needed to release pollensomes, or if they can be secreted also in high humidity conditions; and (II) to investigate the molecular features of pollensomes following the most recent guidelines for EVs isolation and identification.MethodsTo do so, pollensomes were isolated from hydrated and germinated kiwi (Actinidia chinensis Planch.) pollen, and characterized using imaging techniques, immunoblotting, and proteomics.ResultsThese analyses revealed that only germinated kiwi pollen released detectable concentrations of nanoparticles compatible with small EVs for shape and protein content. Moreover, a plant homolog of ALIX, which is a well-recognized and accepted marker of small EVs and exosomes in mammals, was found in pollensomes.DiscussionThe presence of this protein, along with other proteins involved in endocytosis, is consistent with the hypothesis that pollensomes could comprehend a prominent subpopulation of plant exosome-like vesicles.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">pollensomes</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">nanovesicles</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">pollen tube</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">pollination</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">exosomes</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">signaling</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Plant culture</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Elisa Tonoli</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Enzo Fornari</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Maria P. 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Small extracellular vesicles released from germinated kiwi pollen (pollensomes) present characteristics similar to mammalian exosomes and carry a plant homolog of ALIX

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