Linear epitope mapping of the humoral response against SARS-CoV-2 in two independent African cohorts

Abstract Profiling of the antibody responses to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) proteins in African populations is scarce. Here, we performed a detailed IgM and IgG epitope mapping study against 487 peptides covering SARS-CoV-2 wild-type structural proteins. A panel of 4...
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Autor*in:	Inès Vigan-Womas [verfasserIn] Jean-Louis Spadoni [verfasserIn] Thomas Poiret [verfasserIn] Fabien Taïeb [verfasserIn] Fanirisoa Randrianarisaona [verfasserIn] Rokhaya Faye [verfasserIn] Adji Astou Mbow [verfasserIn] Aboubacry Gaye [verfasserIn] Ndongo Dia [verfasserIn] Cheikh Loucoubar [verfasserIn] Diary Juliannie Ny Mioramalala [verfasserIn] Rila Ratovoson [verfasserIn] Rindra Vatosoa Randremanana [verfasserIn] Amadou Alpha Sall [verfasserIn] Moussa Seydi [verfasserIn] Josselin Noirel [verfasserIn] Gabriel Moreau [verfasserIn] Arnaud Simon [verfasserIn] Pavlo Holenya [verfasserIn] Jean-Philippe Meyniel [verfasserIn] Jean-François Zagury [verfasserIn] Matthieu Schoenhals [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Übergeordnetes Werk:	In: Scientific Reports - Nature Portfolio, 2011, 13(2023), 1, Seite 12
Übergeordnetes Werk:	volume:13 ; year:2023 ; number:1 ; pages:12

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1038/s41598-023-27810-1

Katalog-ID:	DOAJ081653875

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allfields	10.1038/s41598-023-27810-1 doi (DE-627)DOAJ081653875 (DE-599)DOAJ2b5c09a85a5e4c7b991d9190800027ff DE-627 ger DE-627 rakwb eng Inès Vigan-Womas verfasserin aut Linear epitope mapping of the humoral response against SARS-CoV-2 in two independent African cohorts 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Profiling of the antibody responses to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) proteins in African populations is scarce. Here, we performed a detailed IgM and IgG epitope mapping study against 487 peptides covering SARS-CoV-2 wild-type structural proteins. A panel of 41 pre-pandemic and 82 COVID-19 RT-PCR confirmed sera from Madagascar and Senegal were used. We found that the main 36 immunodominant linear epitopes identified were (i) similar in both countries, (ii) distributed mainly in the Spike and the Nucleocapsid proteins, (iii) located outside the RBD and NTD regions where most of the reported SARS-CoV-2 variant mutations occur, and (iv) identical to those reported in European, North American, and Asian studies. Within the severe group, antibody levels were inversely correlated with the viral load. This first antibody epitope mapping study performed in patients from two African countries may be helpful to guide rational peptide-based diagnostic assays or vaccine development. Medicine R Science Q Jean-Louis Spadoni verfasserin aut Thomas Poiret verfasserin aut Fabien Taïeb verfasserin aut Fanirisoa Randrianarisaona verfasserin aut Rokhaya Faye verfasserin aut Adji Astou Mbow verfasserin aut Aboubacry Gaye verfasserin aut Ndongo Dia verfasserin aut Cheikh Loucoubar verfasserin aut Diary Juliannie Ny Mioramalala verfasserin aut Rila Ratovoson verfasserin aut Rindra Vatosoa Randremanana verfasserin aut Amadou Alpha Sall verfasserin aut Moussa Seydi verfasserin aut Josselin Noirel verfasserin aut Gabriel Moreau verfasserin aut Arnaud Simon verfasserin aut Pavlo Holenya verfasserin aut Jean-Philippe Meyniel verfasserin aut Jean-François Zagury verfasserin aut Matthieu Schoenhals verfasserin aut In Scientific Reports Nature Portfolio, 2011 13(2023), 1, Seite 12 (DE-627)663366712 (DE-600)2615211-3 20452322 nnns volume:13 year:2023 number:1 pages:12 https://doi.org/10.1038/s41598-023-27810-1 kostenfrei https://doaj.org/article/2b5c09a85a5e4c7b991d9190800027ff kostenfrei https://doi.org/10.1038/s41598-023-27810-1 kostenfrei https://doaj.org/toc/2045-2322 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2023 1 12
spelling	10.1038/s41598-023-27810-1 doi (DE-627)DOAJ081653875 (DE-599)DOAJ2b5c09a85a5e4c7b991d9190800027ff DE-627 ger DE-627 rakwb eng Inès Vigan-Womas verfasserin aut Linear epitope mapping of the humoral response against SARS-CoV-2 in two independent African cohorts 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Profiling of the antibody responses to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) proteins in African populations is scarce. Here, we performed a detailed IgM and IgG epitope mapping study against 487 peptides covering SARS-CoV-2 wild-type structural proteins. A panel of 41 pre-pandemic and 82 COVID-19 RT-PCR confirmed sera from Madagascar and Senegal were used. We found that the main 36 immunodominant linear epitopes identified were (i) similar in both countries, (ii) distributed mainly in the Spike and the Nucleocapsid proteins, (iii) located outside the RBD and NTD regions where most of the reported SARS-CoV-2 variant mutations occur, and (iv) identical to those reported in European, North American, and Asian studies. Within the severe group, antibody levels were inversely correlated with the viral load. This first antibody epitope mapping study performed in patients from two African countries may be helpful to guide rational peptide-based diagnostic assays or vaccine development. Medicine R Science Q Jean-Louis Spadoni verfasserin aut Thomas Poiret verfasserin aut Fabien Taïeb verfasserin aut Fanirisoa Randrianarisaona verfasserin aut Rokhaya Faye verfasserin aut Adji Astou Mbow verfasserin aut Aboubacry Gaye verfasserin aut Ndongo Dia verfasserin aut Cheikh Loucoubar verfasserin aut Diary Juliannie Ny Mioramalala verfasserin aut Rila Ratovoson verfasserin aut Rindra Vatosoa Randremanana verfasserin aut Amadou Alpha Sall verfasserin aut Moussa Seydi verfasserin aut Josselin Noirel verfasserin aut Gabriel Moreau verfasserin aut Arnaud Simon verfasserin aut Pavlo Holenya verfasserin aut Jean-Philippe Meyniel verfasserin aut Jean-François Zagury verfasserin aut Matthieu Schoenhals verfasserin aut In Scientific Reports Nature Portfolio, 2011 13(2023), 1, Seite 12 (DE-627)663366712 (DE-600)2615211-3 20452322 nnns volume:13 year:2023 number:1 pages:12 https://doi.org/10.1038/s41598-023-27810-1 kostenfrei https://doaj.org/article/2b5c09a85a5e4c7b991d9190800027ff kostenfrei https://doi.org/10.1038/s41598-023-27810-1 kostenfrei https://doaj.org/toc/2045-2322 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2023 1 12
allfields_unstemmed	10.1038/s41598-023-27810-1 doi (DE-627)DOAJ081653875 (DE-599)DOAJ2b5c09a85a5e4c7b991d9190800027ff DE-627 ger DE-627 rakwb eng Inès Vigan-Womas verfasserin aut Linear epitope mapping of the humoral response against SARS-CoV-2 in two independent African cohorts 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Profiling of the antibody responses to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) proteins in African populations is scarce. Here, we performed a detailed IgM and IgG epitope mapping study against 487 peptides covering SARS-CoV-2 wild-type structural proteins. A panel of 41 pre-pandemic and 82 COVID-19 RT-PCR confirmed sera from Madagascar and Senegal were used. We found that the main 36 immunodominant linear epitopes identified were (i) similar in both countries, (ii) distributed mainly in the Spike and the Nucleocapsid proteins, (iii) located outside the RBD and NTD regions where most of the reported SARS-CoV-2 variant mutations occur, and (iv) identical to those reported in European, North American, and Asian studies. Within the severe group, antibody levels were inversely correlated with the viral load. This first antibody epitope mapping study performed in patients from two African countries may be helpful to guide rational peptide-based diagnostic assays or vaccine development. Medicine R Science Q Jean-Louis Spadoni verfasserin aut Thomas Poiret verfasserin aut Fabien Taïeb verfasserin aut Fanirisoa Randrianarisaona verfasserin aut Rokhaya Faye verfasserin aut Adji Astou Mbow verfasserin aut Aboubacry Gaye verfasserin aut Ndongo Dia verfasserin aut Cheikh Loucoubar verfasserin aut Diary Juliannie Ny Mioramalala verfasserin aut Rila Ratovoson verfasserin aut Rindra Vatosoa Randremanana verfasserin aut Amadou Alpha Sall verfasserin aut Moussa Seydi verfasserin aut Josselin Noirel verfasserin aut Gabriel Moreau verfasserin aut Arnaud Simon verfasserin aut Pavlo Holenya verfasserin aut Jean-Philippe Meyniel verfasserin aut Jean-François Zagury verfasserin aut Matthieu Schoenhals verfasserin aut In Scientific Reports Nature Portfolio, 2011 13(2023), 1, Seite 12 (DE-627)663366712 (DE-600)2615211-3 20452322 nnns volume:13 year:2023 number:1 pages:12 https://doi.org/10.1038/s41598-023-27810-1 kostenfrei https://doaj.org/article/2b5c09a85a5e4c7b991d9190800027ff kostenfrei https://doi.org/10.1038/s41598-023-27810-1 kostenfrei https://doaj.org/toc/2045-2322 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2023 1 12
allfieldsGer	10.1038/s41598-023-27810-1 doi (DE-627)DOAJ081653875 (DE-599)DOAJ2b5c09a85a5e4c7b991d9190800027ff DE-627 ger DE-627 rakwb eng Inès Vigan-Womas verfasserin aut Linear epitope mapping of the humoral response against SARS-CoV-2 in two independent African cohorts 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Profiling of the antibody responses to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) proteins in African populations is scarce. Here, we performed a detailed IgM and IgG epitope mapping study against 487 peptides covering SARS-CoV-2 wild-type structural proteins. A panel of 41 pre-pandemic and 82 COVID-19 RT-PCR confirmed sera from Madagascar and Senegal were used. We found that the main 36 immunodominant linear epitopes identified were (i) similar in both countries, (ii) distributed mainly in the Spike and the Nucleocapsid proteins, (iii) located outside the RBD and NTD regions where most of the reported SARS-CoV-2 variant mutations occur, and (iv) identical to those reported in European, North American, and Asian studies. Within the severe group, antibody levels were inversely correlated with the viral load. This first antibody epitope mapping study performed in patients from two African countries may be helpful to guide rational peptide-based diagnostic assays or vaccine development. Medicine R Science Q Jean-Louis Spadoni verfasserin aut Thomas Poiret verfasserin aut Fabien Taïeb verfasserin aut Fanirisoa Randrianarisaona verfasserin aut Rokhaya Faye verfasserin aut Adji Astou Mbow verfasserin aut Aboubacry Gaye verfasserin aut Ndongo Dia verfasserin aut Cheikh Loucoubar verfasserin aut Diary Juliannie Ny Mioramalala verfasserin aut Rila Ratovoson verfasserin aut Rindra Vatosoa Randremanana verfasserin aut Amadou Alpha Sall verfasserin aut Moussa Seydi verfasserin aut Josselin Noirel verfasserin aut Gabriel Moreau verfasserin aut Arnaud Simon verfasserin aut Pavlo Holenya verfasserin aut Jean-Philippe Meyniel verfasserin aut Jean-François Zagury verfasserin aut Matthieu Schoenhals verfasserin aut In Scientific Reports Nature Portfolio, 2011 13(2023), 1, Seite 12 (DE-627)663366712 (DE-600)2615211-3 20452322 nnns volume:13 year:2023 number:1 pages:12 https://doi.org/10.1038/s41598-023-27810-1 kostenfrei https://doaj.org/article/2b5c09a85a5e4c7b991d9190800027ff kostenfrei https://doi.org/10.1038/s41598-023-27810-1 kostenfrei https://doaj.org/toc/2045-2322 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2023 1 12
allfieldsSound	10.1038/s41598-023-27810-1 doi (DE-627)DOAJ081653875 (DE-599)DOAJ2b5c09a85a5e4c7b991d9190800027ff DE-627 ger DE-627 rakwb eng Inès Vigan-Womas verfasserin aut Linear epitope mapping of the humoral response against SARS-CoV-2 in two independent African cohorts 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Profiling of the antibody responses to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) proteins in African populations is scarce. Here, we performed a detailed IgM and IgG epitope mapping study against 487 peptides covering SARS-CoV-2 wild-type structural proteins. A panel of 41 pre-pandemic and 82 COVID-19 RT-PCR confirmed sera from Madagascar and Senegal were used. We found that the main 36 immunodominant linear epitopes identified were (i) similar in both countries, (ii) distributed mainly in the Spike and the Nucleocapsid proteins, (iii) located outside the RBD and NTD regions where most of the reported SARS-CoV-2 variant mutations occur, and (iv) identical to those reported in European, North American, and Asian studies. Within the severe group, antibody levels were inversely correlated with the viral load. This first antibody epitope mapping study performed in patients from two African countries may be helpful to guide rational peptide-based diagnostic assays or vaccine development. Medicine R Science Q Jean-Louis Spadoni verfasserin aut Thomas Poiret verfasserin aut Fabien Taïeb verfasserin aut Fanirisoa Randrianarisaona verfasserin aut Rokhaya Faye verfasserin aut Adji Astou Mbow verfasserin aut Aboubacry Gaye verfasserin aut Ndongo Dia verfasserin aut Cheikh Loucoubar verfasserin aut Diary Juliannie Ny Mioramalala verfasserin aut Rila Ratovoson verfasserin aut Rindra Vatosoa Randremanana verfasserin aut Amadou Alpha Sall verfasserin aut Moussa Seydi verfasserin aut Josselin Noirel verfasserin aut Gabriel Moreau verfasserin aut Arnaud Simon verfasserin aut Pavlo Holenya verfasserin aut Jean-Philippe Meyniel verfasserin aut Jean-François Zagury verfasserin aut Matthieu Schoenhals verfasserin aut In Scientific Reports Nature Portfolio, 2011 13(2023), 1, Seite 12 (DE-627)663366712 (DE-600)2615211-3 20452322 nnns volume:13 year:2023 number:1 pages:12 https://doi.org/10.1038/s41598-023-27810-1 kostenfrei https://doaj.org/article/2b5c09a85a5e4c7b991d9190800027ff kostenfrei https://doi.org/10.1038/s41598-023-27810-1 kostenfrei https://doaj.org/toc/2045-2322 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2023 1 12
language	English
source	In Scientific Reports 13(2023), 1, Seite 12 volume:13 year:2023 number:1 pages:12
sourceStr	In Scientific Reports 13(2023), 1, Seite 12 volume:13 year:2023 number:1 pages:12
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Medicine R Science Q
isfreeaccess_bool	true
container_title	Scientific Reports
authorswithroles_txt_mv	Inès Vigan-Womas @@aut@@ Jean-Louis Spadoni @@aut@@ Thomas Poiret @@aut@@ Fabien Taïeb @@aut@@ Fanirisoa Randrianarisaona @@aut@@ Rokhaya Faye @@aut@@ Adji Astou Mbow @@aut@@ Aboubacry Gaye @@aut@@ Ndongo Dia @@aut@@ Cheikh Loucoubar @@aut@@ Diary Juliannie Ny Mioramalala @@aut@@ Rila Ratovoson @@aut@@ Rindra Vatosoa Randremanana @@aut@@ Amadou Alpha Sall @@aut@@ Moussa Seydi @@aut@@ Josselin Noirel @@aut@@ Gabriel Moreau @@aut@@ Arnaud Simon @@aut@@ Pavlo Holenya @@aut@@ Jean-Philippe Meyniel @@aut@@ Jean-François Zagury @@aut@@ Matthieu Schoenhals @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
hierarchy_top_id	663366712
id	DOAJ081653875
language_de	englisch
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author	Inès Vigan-Womas
spellingShingle	Inès Vigan-Womas misc Medicine misc R misc Science misc Q Linear epitope mapping of the humoral response against SARS-CoV-2 in two independent African cohorts
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topic_title	Linear epitope mapping of the humoral response against SARS-CoV-2 in two independent African cohorts
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title	Linear epitope mapping of the humoral response against SARS-CoV-2 in two independent African cohorts
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title_full	Linear epitope mapping of the humoral response against SARS-CoV-2 in two independent African cohorts
author_sort	Inès Vigan-Womas
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author_browse	Inès Vigan-Womas Jean-Louis Spadoni Thomas Poiret Fabien Taïeb Fanirisoa Randrianarisaona Rokhaya Faye Adji Astou Mbow Aboubacry Gaye Ndongo Dia Cheikh Loucoubar Diary Juliannie Ny Mioramalala Rila Ratovoson Rindra Vatosoa Randremanana Amadou Alpha Sall Moussa Seydi Josselin Noirel Gabriel Moreau Arnaud Simon Pavlo Holenya Jean-Philippe Meyniel Jean-François Zagury Matthieu Schoenhals
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title_sort	linear epitope mapping of the humoral response against sars-cov-2 in two independent african cohorts
title_auth	Linear epitope mapping of the humoral response against SARS-CoV-2 in two independent African cohorts
abstract	Abstract Profiling of the antibody responses to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) proteins in African populations is scarce. Here, we performed a detailed IgM and IgG epitope mapping study against 487 peptides covering SARS-CoV-2 wild-type structural proteins. A panel of 41 pre-pandemic and 82 COVID-19 RT-PCR confirmed sera from Madagascar and Senegal were used. We found that the main 36 immunodominant linear epitopes identified were (i) similar in both countries, (ii) distributed mainly in the Spike and the Nucleocapsid proteins, (iii) located outside the RBD and NTD regions where most of the reported SARS-CoV-2 variant mutations occur, and (iv) identical to those reported in European, North American, and Asian studies. Within the severe group, antibody levels were inversely correlated with the viral load. This first antibody epitope mapping study performed in patients from two African countries may be helpful to guide rational peptide-based diagnostic assays or vaccine development.
abstractGer	Abstract Profiling of the antibody responses to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) proteins in African populations is scarce. Here, we performed a detailed IgM and IgG epitope mapping study against 487 peptides covering SARS-CoV-2 wild-type structural proteins. A panel of 41 pre-pandemic and 82 COVID-19 RT-PCR confirmed sera from Madagascar and Senegal were used. We found that the main 36 immunodominant linear epitopes identified were (i) similar in both countries, (ii) distributed mainly in the Spike and the Nucleocapsid proteins, (iii) located outside the RBD and NTD regions where most of the reported SARS-CoV-2 variant mutations occur, and (iv) identical to those reported in European, North American, and Asian studies. Within the severe group, antibody levels were inversely correlated with the viral load. This first antibody epitope mapping study performed in patients from two African countries may be helpful to guide rational peptide-based diagnostic assays or vaccine development.
abstract_unstemmed	Abstract Profiling of the antibody responses to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) proteins in African populations is scarce. Here, we performed a detailed IgM and IgG epitope mapping study against 487 peptides covering SARS-CoV-2 wild-type structural proteins. A panel of 41 pre-pandemic and 82 COVID-19 RT-PCR confirmed sera from Madagascar and Senegal were used. We found that the main 36 immunodominant linear epitopes identified were (i) similar in both countries, (ii) distributed mainly in the Spike and the Nucleocapsid proteins, (iii) located outside the RBD and NTD regions where most of the reported SARS-CoV-2 variant mutations occur, and (iv) identical to those reported in European, North American, and Asian studies. Within the severe group, antibody levels were inversely correlated with the viral load. This first antibody epitope mapping study performed in patients from two African countries may be helpful to guide rational peptide-based diagnostic assays or vaccine development.
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title_short	Linear epitope mapping of the humoral response against SARS-CoV-2 in two independent African cohorts
url	https://doi.org/10.1038/s41598-023-27810-1 https://doaj.org/article/2b5c09a85a5e4c7b991d9190800027ff https://doaj.org/toc/2045-2322
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author2	Jean-Louis Spadoni Thomas Poiret Fabien Taïeb Fanirisoa Randrianarisaona Rokhaya Faye Adji Astou Mbow Aboubacry Gaye Ndongo Dia Cheikh Loucoubar Diary Juliannie Ny Mioramalala Rila Ratovoson Rindra Vatosoa Randremanana Amadou Alpha Sall Moussa Seydi Josselin Noirel Gabriel Moreau Arnaud Simon Pavlo Holenya Jean-Philippe Meyniel Jean-François Zagury Matthieu Schoenhals
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Linear epitope mapping of the humoral response against SARS-CoV-2 in two independent African cohorts

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